Nos2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nos2
Entrez Gene ID	18126
Full Name	nitric oxide synthase 2, inducible
Synonyms	MAC-NOS,NOS-II,Nos-2,Nos2a,i-NOS,iNOS
General protein information	Preferred Names nitric oxide synthase 2, inducible Names nitric oxide synthase, inducible NOS type II inducible NO synthase inducible NOS inducible nitric oxide synthase macrophage NOS nitric oxide synthase 2, inducible, macrophage peptidyl-cysteine S-nitrosylase NOS2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 46.74 cM
Summary	Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001313921.1	NP_001300850.1	nitric oxide synthase, inducible isoform b
NM_010927.3	NP_035057.1	nitric oxide synthase, inducible
NM_010927.4	NP_035057.1	nitric oxide synthase, inducible isoform a
NM_001313922.1	NP_001300851.1	nitric oxide synthase, inducible isoform b
XM_006532446.3	XP_006532509.1	nitric oxide synthase, inducible isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NOS2	NP_001070267.1
Canis lupus familiaris (dog)	NOS2	XP_005624903.1
Xenopus tropicalis (tropical clawed frog)	nos2	XP_002935342.2
Macaca mulatta (Rhesus monkey)	NOS2	XP_001106245.1
Rattus norvegicus (Norway rat)	Nos2	NP_036743.3
Danio rerio (zebrafish)	nos2a	NP_001098407.1
Homo sapiens (human)	NOS2	NP_000616.3
Rattus norvegicus (Norway rat)	LOC497963	XP_006247011.1
Gallus gallus (chicken)	NOS2	NP_990292.1
Pan troglodytes (chimpanzee)	NOS2	XP_001148238.1
Mus musculus (house mouse)	Nos2	NP_035057.1
Danio rerio (zebrafish)	nos2b	NP_001106973.1

Gene Ontology
Bibliography

Related articles in PubMed

Brucella abortus nitric oxide metabolite regulates inflammasome activation and IL-1β secretion in murine macrophages.
Campos PC, Gomes MTR, Marinho FAV, Guimarães ES, de Moura Lodi Cruz MGF, Oliveira SC
European journal of immunology49(7)1023-1037(2019 Jul)

IFN-γ-dependent nitric oxide suppresses Brucella-induced arthritis by inhibition of inflammasome activation.
Lacey CA, Chambers CA, Mitchell WJ, Skyberg JA
Journal of leukocyte biology106(1)27-34(2019 Jul)

Mechanism and regulation of ferrous heme-nitric oxide (NO) oxidation in NO synthases.
Tejero J, Hunt AP, Santolini J, Lehnert N, Stuehr DJ
The Journal of biological chemistry294(19)7904-7916(2019 05)

Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive- and anxiety-like behaviour, and gut microbiome composition.
Inserra A, Choo JM, Lewis MD, Rogers GB, Wong ML, Licinio J
Scientific reports9(1)6456(2019 Apr)

Transcriptional Regulation of Nos2 via STAT5B Binding to Nos2 Gene Promoter Mediates Nitric Oxide Production: Relevance in β-Cell Maintenance.
Joseph A, Nair LCR, Johnson BS, Thomas PL, Padmanabhan RA, Puthumadathil N, Laloraya M
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology52(1)141-155(2019)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nos2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nos2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23261	NM_010927.4 Latest version!	Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu83579	NM_001313921.1 Latest version!	Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu83579	NM_001313922.1 Latest version!	Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu45711	XM_006532446.3 Latest version!	Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu23261	NM_010927.3	Mus musculus nitric oxide synthase 2, inducible (Nos2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nos2 ( NM_010927.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035057.1 Mus musculus nitric oxide synthase 2, inducible (Nos2), mRNA.

Price & Availability↑

CloneID	OMu23261
Clone ID Related Accession (Same CDS sequence)	NM_010927.3 , NM_010927.4
Accession Version	NM_010927.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1423843200000
Organism	Mus musculus(House mouse)
Product	nitric oxide synthase, inducible
Comment

ATGGCTTGCC CCTGGAAGTT TCTCTTCAAA GTCAAATCCT ACCAAAGTGA CCTGAAAGAG 
GAAAAGGACA TTAACAACAA CGTGAAGAAA ACCCCTTGTG CTGTTCTCAG CCCAACAATA 
CAAGATGACC CTAAGAGTCA CCAAAATGGC TCCCCGCAGC TCCTCACTGG GACAGCACAG 
AATGTTCCAG AATCCCTGGA CAAGCTGCAT GTGACATCGA CCCGTCCACA GTATGTGAGG 
ATCAAAAACT GGGGCAGTGG AGAGATTTTG CATGACACTC TTCACCACAA GGCCACATCG 
GATTTCACTT GCAAGTCCAA GTCTTGCTTG GGGTCCATCA TGAACCCCAA GAGTTTGACC 
AGAGGACCCA GAGACAAGCC TACCCCTCTG GAGGAGCTCC TGCCTCATGC CATTGAGTTC 
ATCAACCAGT ATTATGGCTC CTTTAAAGAG GCAAAAATAG AGGAACATCT GGCCAGGCTG 
GAAGCTGTAA CAAAGGAAAT AGAAACAACA GGAACCTACC AGCTCACTCT GGATGAGCTC 
ATCTTTGCCA CCAAGATGGC CTGGAGGAAT GCCCCTCGCT GCATCGGCAG GATCCAGTGG 
TCCAACCTGC AGGTCTTTGA CGCTCGGAAC TGTAGCACAG CACAGGAAAT GTTTCAGCAC 
ATCTGCAGAC ACATACTTTA TGCCACCAAC AATGGCAACA TCAGGTCGGC CATCACTGTG 
TTCCCCCAGC GGAGTGACGG CAAACATGAC TTCAGGCTCT GGAATTCACA GCTCATCCGG 
TACGCTGGCT ACCAGATGCC CGATGGCACC ATCAGAGGGG ATGCTGCCAC CTTGGAGTTC 
ACCCAGTTGT GCATCGACCT AGGCTGGAAG CCCCGCTATG GCCGCTTTGA TGTGCTGCCT 
CTGGTCTTGC AAGCTGATGG TCAAGATCCA GAGGTCTTTG AAATCCCTCC TGATCTTGTG 
TTGGAGGTGA CCATGGAGCA TCCCAAGTAC GAGTGGTTCC AGGAGCTCGG GTTGAAGTGG 
TATGCACTGC CTGCCGTGGC CAACATGCTA CTGGAGGTGG GTGGCCTCGA ATTCCCAGCC 
TGCCCCTTCA ATGGTTGGTA CATGGGCACC GAGATTGGAG TTCGAGACTT CTGTGACACA 
CAGCGCTACA ACATCCTGGA GGAAGTGGGC CGAAGGATGG GCCTGGAGAC CCACACACTG 
GCCTCCCTCT GGAAAGACCG GGCTGTCACG GAGATCAATG TGGCTGTGCT CCATAGTTTC 
CAGAAGCAGA ATGTGACCAT CATGGACCAC CACACAGCCT CAGAGTCCTT CATGAAGCAC 
ATGCAGAATG AGTACCGGGC CCGTGGAGGC TGCCCGGCAG ACTGGATTTG GCTGGTCCCT 
CCAGTGTCTG GGAGCATCAC CCCTGTGTTC CACCAGGAGA TGTTGAACTA TGTCCTATCT 
CCATTCTACT ACTACCAGAT CGAGCCCTGG AAGACCCACA TCTGGCAGAA TGAGAAGCTG 
AGGCCCAGGA GGAGAGAGAT CCGATTTAGA GTCTTGGTGA AAGTGGTGTT CTTTGCTTCC 
ATGCTAATGC GAAAGGTCAT GGCTTCACGG GTCAGAGCCA CAGTCCTCTT TGCTACTGAG 
ACAGGGAAGT CTGAAGCACT AGCCAGGGAC CTGGCCACCT TGTTCAGCTA CGCCTTCAAC 
ACCAAGGTTG TCTGCATGGA CCAGTATAAG GCAAGCACCT TGGAAGAGGA GCAACTACTG 
CTGGTGGTGA CAAGCACATT TGGGAATGGA GACTGTCCCA GCAATGGGCA GACTCTGAAG 
AAATCTCTGT TCATGCTTAG AGAACTCAAC CACACCTTCA GGTATGCTGT GTTTGGCCTT 
GGCTCCAGCA TGTACCCTCA GTTCTGCGCC TTTGCTCATG ACATCGACCA GAAGCTGTCC 
CACCTGGGAG CCTCTCAGCT TGCCCCAACA GGAGAAGGGG ACGAACTCAG TGGGCAGGAG 
GATGCCTTCC GCAGCTGGGC TGTACAAACC TTCCGGGCAG CCTGTGAGAC CTTTGATGTC 
CGAAGCAAAC ATCACATTCA GATCCCGAAA CGCTTCACTT CCAATGCAAC ATGGGAGCCA 
CAGCAATATA GGCTCATCCA GAGCCCGGAG CCTTTAGACC TCAACAGAGC CCTCAGCAGC 
ATCCATGCAA AGAACGTGTT TACCATGAGG CTGAAATCCC AGCAGAATCT GCAGAGTGAA 
AAGTCCAGCC GCACCACCCT CCTCGTTCAG CTCACCTTCG AGGGCAGCCG AGGGCCCAGC 
TACCTGCCTG GGGAACACCT GGGGATCTTC CCAGGCAACC AGACCGCCCT GGTGCAGGGA 
ATCTTGGAGC GAGTTGTGGA TTGTCCTACA CCACACCAAA CTGTGTGCCT GGAGGTTCTG 
GATGAGAGCG GCAGCTACTG GGTCAAAGAC AAGAGGCTGC CCCCCTGCTC ACTCAGCCAA 
GCCCTCACCT ACTTCCTGGA CATTACGACC CCTCCCACCC AGCTGCAGCT CCACAAGCTG 
GCTCGCTTTG CCACGGACGA GACGGATAGG CAGAGATTGG AGGCCTTGTG TCAGCCCTCA 
GAGTACAATG ACTGGAAGTT CAGCAACAAC CCCACGTTCC TGGAGGTGCT TGAAGAGTTC 
CCTTCCTTGC ATGTGCCCGC TGCCTTCCTG CTGTCGCAGC TCCCTATCTT GAAGCCCCGC 
TACTACTCCA TCAGCTCCTC CCAGGACCAC ACCCCCTCGG AGGTTCACCT CACTGTGGCC 
GTGGTCACCT ACCGCACCCG AGATGGTCAG GGTCCCCTGC ACCATGGTGT CTGCAGCACT 
TGGATCAGGA ACCTGAAGCC CCAGGACCCA GTGCCCTGCT TTGTGCGAAG TGTCAGTGGC 
TTCCAGCTCC CTGAGGACCC CTCCCAGCCT TGCATCCTCA TTGGGCCTGG TACGGGCATT 
GCTCCCTTCC GAAGTTTCTG GCAGCAGCGG CTCCATGACT CCCAGCACAA AGGGCTCAAA 
GGAGGCCGCA TGAGCTTGGT GTTTGGGTGC CGGCACCCGG AGGAGGACCA CCTCTATCAG 
GAAGAAATGC AGGAGATGGT CCGCAAGAGA GTGCTGTTCC AGGTGCACAC AGGCTACTCC 
CGGCTGCCCG GCAAACCCAA GGTCTACGTT CAGGACATCC TGCAAAAGCA GCTGGCCAAT 
GAGGTACTCA GCGTGCTCCA CGGGGAGCAG GGCCACCTCT ACATTTGCGG AGATGTGCGC 
ATGGCTCGGG ATGTGGCTAC CACATTGAAG AAGCTGGTGG CCACCAAGCT GAACTTGAGC 
GAGGAGCAGG TGGAAGACTA TTTCTTCCAG CTCAAGAGCC AGAAACGTTA TCATGAAGAT 
ATCTTCGGTG CAGTCTTTTC CTATGGGGCA AAAAAGGGCA GCGCCTTGGA GGAGCCCAAA 
GCCACGAGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_035057.1

Translation

MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLHVTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEFINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARNCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEFTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANMLLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSFQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPWKTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFNTKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEPQQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIFPGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKLARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDHTPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGIAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYVQDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHEDIFGAVFSYGAKKGSALEEPKATRL

Target ORF information:

RefSeq Version	NM_010927.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus nitric oxide synthase 2, inducible (Nos2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010927.3

ORF Insert Sequence:

ATGGCTTGCC CCTGGAAGTT TCTCTTCAAA GTCAAATCCT ACCAAAGTGA CCTGAAAGAG 
GAAAAGGACA TTAACAACAA CGTGAAGAAA ACCCCTTGTG CTGTTCTCAG CCCAACAATA 
CAAGATGACC CTAAGAGTCA CCAAAATGGC TCCCCGCAGC TCCTCACTGG GACAGCACAG 
AATGTTCCAG AATCCCTGGA CAAGCTGCAT GTGACATCGA CCCGTCCACA GTATGTGAGG 
ATCAAAAACT GGGGCAGTGG AGAGATTTTG CATGACACTC TTCACCACAA GGCCACATCG 
GATTTCACTT GCAAGTCCAA GTCTTGCTTG GGGTCCATCA TGAACCCCAA GAGTTTGACC 
AGAGGACCCA GAGACAAGCC TACCCCTCTG GAGGAGCTCC TGCCTCATGC CATTGAGTTC 
ATCAACCAGT ATTATGGCTC CTTTAAAGAG GCAAAAATAG AGGAACATCT GGCCAGGCTG 
GAAGCTGTAA CAAAGGAAAT AGAAACAACA GGAACCTACC AGCTCACTCT GGATGAGCTC 
ATCTTTGCCA CCAAGATGGC CTGGAGGAAT GCCCCTCGCT GCATCGGCAG GATCCAGTGG 
TCCAACCTGC AGGTCTTTGA CGCTCGGAAC TGTAGCACAG CACAGGAAAT GTTTCAGCAC 
ATCTGCAGAC ACATACTTTA TGCCACCAAC AATGGCAACA TCAGGTCGGC CATCACTGTG 
TTCCCCCAGC GGAGTGACGG CAAACATGAC TTCAGGCTCT GGAATTCACA GCTCATCCGG 
TACGCTGGCT ACCAGATGCC CGATGGCACC ATCAGAGGGG ATGCTGCCAC CTTGGAGTTC 
ACCCAGTTGT GCATCGACCT AGGCTGGAAG CCCCGCTATG GCCGCTTTGA TGTGCTGCCT 
CTGGTCTTGC AAGCTGATGG TCAAGATCCA GAGGTCTTTG AAATCCCTCC TGATCTTGTG 
TTGGAGGTGA CCATGGAGCA TCCCAAGTAC GAGTGGTTCC AGGAGCTCGG GTTGAAGTGG 
TATGCACTGC CTGCCGTGGC CAACATGCTA CTGGAGGTGG GTGGCCTCGA ATTCCCAGCC 
TGCCCCTTCA ATGGTTGGTA CATGGGCACC GAGATTGGAG TTCGAGACTT CTGTGACACA 
CAGCGCTACA ACATCCTGGA GGAAGTGGGC CGAAGGATGG GCCTGGAGAC CCACACACTG 
GCCTCCCTCT GGAAAGACCG GGCTGTCACG GAGATCAATG TGGCTGTGCT CCATAGTTTC 
CAGAAGCAGA ATGTGACCAT CATGGACCAC CACACAGCCT CAGAGTCCTT CATGAAGCAC 
ATGCAGAATG AGTACCGGGC CCGTGGAGGC TGCCCGGCAG ACTGGATTTG GCTGGTCCCT 
CCAGTGTCTG GGAGCATCAC CCCTGTGTTC CACCAGGAGA TGTTGAACTA TGTCCTATCT 
CCATTCTACT ACTACCAGAT CGAGCCCTGG AAGACCCACA TCTGGCAGAA TGAGAAGCTG 
AGGCCCAGGA GGAGAGAGAT CCGATTTAGA GTCTTGGTGA AAGTGGTGTT CTTTGCTTCC 
ATGCTAATGC GAAAGGTCAT GGCTTCACGG GTCAGAGCCA CAGTCCTCTT TGCTACTGAG 
ACAGGGAAGT CTGAAGCACT AGCCAGGGAC CTGGCCACCT TGTTCAGCTA CGCCTTCAAC 
ACCAAGGTTG TCTGCATGGA CCAGTATAAG GCAAGCACCT TGGAAGAGGA GCAACTACTG 
CTGGTGGTGA CAAGCACATT TGGGAATGGA GACTGTCCCA GCAATGGGCA GACTCTGAAG 
AAATCTCTGT TCATGCTTAG AGAACTCAAC CACACCTTCA GGTATGCTGT GTTTGGCCTT 
GGCTCCAGCA TGTACCCTCA GTTCTGCGCC TTTGCTCATG ACATCGACCA GAAGCTGTCC 
CACCTGGGAG CCTCTCAGCT TGCCCCAACA GGAGAAGGGG ACGAACTCAG TGGGCAGGAG 
GATGCCTTCC GCAGCTGGGC TGTACAAACC TTCCGGGCAG CCTGTGAGAC CTTTGATGTC 
CGAAGCAAAC ATCACATTCA GATCCCGAAA CGCTTCACTT CCAATGCAAC ATGGGAGCCA 
CAGCAATATA GGCTCATCCA GAGCCCGGAG CCTTTAGACC TCAACAGAGC CCTCAGCAGC 
ATCCATGCAA AGAACGTGTT TACCATGAGG CTGAAATCCC AGCAGAATCT GCAGAGTGAA 
AAGTCCAGCC GCACCACCCT CCTCGTTCAG CTCACCTTCG AGGGCAGCCG AGGGCCCAGC 
TACCTGCCTG GGGAACACCT GGGGATCTTC CCAGGCAACC AGACCGCCCT GGTGCAGGGA 
ATCTTGGAGC GAGTTGTGGA TTGTCCTACA CCACACCAAA CTGTGTGCCT GGAGGTTCTG 
GATGAGAGCG GCAGCTACTG GGTCAAAGAC AAGAGGCTGC CCCCCTGCTC ACTCAGCCAA 
GCCCTCACCT ACTTCCTGGA CATTACGACC CCTCCCACCC AGCTGCAGCT CCACAAGCTG 
GCTCGCTTTG CCACGGACGA GACGGATAGG CAGAGATTGG AGGCCTTGTG TCAGCCCTCA 
GAGTACAATG ACTGGAAGTT CAGCAACAAC CCCACGTTCC TGGAGGTGCT TGAAGAGTTC 
CCTTCCTTGC ATGTGCCCGC TGCCTTCCTG CTGTCGCAGC TCCCTATCTT GAAGCCCCGC 
TACTACTCCA TCAGCTCCTC CCAGGACCAC ACCCCCTCGG AGGTTCACCT CACTGTGGCC 
GTGGTCACCT ACCGCACCCG AGATGGTCAG GGTCCCCTGC ACCATGGTGT CTGCAGCACT 
TGGATCAGGA ACCTGAAGCC CCAGGACCCA GTGCCCTGCT TTGTGCGAAG TGTCAGTGGC 
TTCCAGCTCC CTGAGGACCC CTCCCAGCCT TGCATCCTCA TTGGGCCTGG TACGGGCATT 
GCTCCCTTCC GAAGTTTCTG GCAGCAGCGG CTCCATGACT CCCAGCACAA AGGGCTCAAA 
GGAGGCCGCA TGAGCTTGGT GTTTGGGTGC CGGCACCCGG AGGAGGACCA CCTCTATCAG 
GAAGAAATGC AGGAGATGGT CCGCAAGAGA GTGCTGTTCC AGGTGCACAC AGGCTACTCC 
CGGCTGCCCG GCAAACCCAA GGTCTACGTT CAGGACATCC TGCAAAAGCA GCTGGCCAAT 
GAGGTACTCA GCGTGCTCCA CGGGGAGCAG GGCCACCTCT ACATTTGCGG AGATGTGCGC 
ATGGCTCGGG ATGTGGCTAC CACATTGAAG AAGCTGGTGG CCACCAAGCT GAACTTGAGC 
GAGGAGCAGG TGGAAGACTA TTTCTTCCAG CTCAAGAGCC AGAAACGTTA TCATGAAGAT 
ATCTTCGGTG CAGTCTTTTC CTATGGGGCA AAAAAGGGCA GCGCCTTGGA GGAGCCCAAA 
GCCACGAGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos2 ( NM_010927.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035057.1 Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23261
Clone ID Related Accession (Same CDS sequence)	NM_010927.3 , NM_010927.4
Accession Version	NM_010927.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577462400000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, inducible isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M87039.1 and AL592185.35. On Sep 11, 2015 this sequence version replaced NM_010927.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M87039.1, M84373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTGCC CCTGGAAGTT TCTCTTCAAA GTCAAATCCT ACCAAAGTGA CCTGAAAGAG 
GAAAAGGACA TTAACAACAA CGTGAAGAAA ACCCCTTGTG CTGTTCTCAG CCCAACAATA 
CAAGATGACC CTAAGAGTCA CCAAAATGGC TCCCCGCAGC TCCTCACTGG GACAGCACAG 
AATGTTCCAG AATCCCTGGA CAAGCTGCAT GTGACATCGA CCCGTCCACA GTATGTGAGG 
ATCAAAAACT GGGGCAGTGG AGAGATTTTG CATGACACTC TTCACCACAA GGCCACATCG 
GATTTCACTT GCAAGTCCAA GTCTTGCTTG GGGTCCATCA TGAACCCCAA GAGTTTGACC 
AGAGGACCCA GAGACAAGCC TACCCCTCTG GAGGAGCTCC TGCCTCATGC CATTGAGTTC 
ATCAACCAGT ATTATGGCTC CTTTAAAGAG GCAAAAATAG AGGAACATCT GGCCAGGCTG 
GAAGCTGTAA CAAAGGAAAT AGAAACAACA GGAACCTACC AGCTCACTCT GGATGAGCTC 
ATCTTTGCCA CCAAGATGGC CTGGAGGAAT GCCCCTCGCT GCATCGGCAG GATCCAGTGG 
TCCAACCTGC AGGTCTTTGA CGCTCGGAAC TGTAGCACAG CACAGGAAAT GTTTCAGCAC 
ATCTGCAGAC ACATACTTTA TGCCACCAAC AATGGCAACA TCAGGTCGGC CATCACTGTG 
TTCCCCCAGC GGAGTGACGG CAAACATGAC TTCAGGCTCT GGAATTCACA GCTCATCCGG 
TACGCTGGCT ACCAGATGCC CGATGGCACC ATCAGAGGGG ATGCTGCCAC CTTGGAGTTC 
ACCCAGTTGT GCATCGACCT AGGCTGGAAG CCCCGCTATG GCCGCTTTGA TGTGCTGCCT 
CTGGTCTTGC AAGCTGATGG TCAAGATCCA GAGGTCTTTG AAATCCCTCC TGATCTTGTG 
TTGGAGGTGA CCATGGAGCA TCCCAAGTAC GAGTGGTTCC AGGAGCTCGG GTTGAAGTGG 
TATGCACTGC CTGCCGTGGC CAACATGCTA CTGGAGGTGG GTGGCCTCGA ATTCCCAGCC 
TGCCCCTTCA ATGGTTGGTA CATGGGCACC GAGATTGGAG TTCGAGACTT CTGTGACACA 
CAGCGCTACA ACATCCTGGA GGAAGTGGGC CGAAGGATGG GCCTGGAGAC CCACACACTG 
GCCTCCCTCT GGAAAGACCG GGCTGTCACG GAGATCAATG TGGCTGTGCT CCATAGTTTC 
CAGAAGCAGA ATGTGACCAT CATGGACCAC CACACAGCCT CAGAGTCCTT CATGAAGCAC 
ATGCAGAATG AGTACCGGGC CCGTGGAGGC TGCCCGGCAG ACTGGATTTG GCTGGTCCCT 
CCAGTGTCTG GGAGCATCAC CCCTGTGTTC CACCAGGAGA TGTTGAACTA TGTCCTATCT 
CCATTCTACT ACTACCAGAT CGAGCCCTGG AAGACCCACA TCTGGCAGAA TGAGAAGCTG 
AGGCCCAGGA GGAGAGAGAT CCGATTTAGA GTCTTGGTGA AAGTGGTGTT CTTTGCTTCC 
ATGCTAATGC GAAAGGTCAT GGCTTCACGG GTCAGAGCCA CAGTCCTCTT TGCTACTGAG 
ACAGGGAAGT CTGAAGCACT AGCCAGGGAC CTGGCCACCT TGTTCAGCTA CGCCTTCAAC 
ACCAAGGTTG TCTGCATGGA CCAGTATAAG GCAAGCACCT TGGAAGAGGA GCAACTACTG 
CTGGTGGTGA CAAGCACATT TGGGAATGGA GACTGTCCCA GCAATGGGCA GACTCTGAAG 
AAATCTCTGT TCATGCTTAG AGAACTCAAC CACACCTTCA GGTATGCTGT GTTTGGCCTT 
GGCTCCAGCA TGTACCCTCA GTTCTGCGCC TTTGCTCATG ACATCGACCA GAAGCTGTCC 
CACCTGGGAG CCTCTCAGCT TGCCCCAACA GGAGAAGGGG ACGAACTCAG TGGGCAGGAG 
GATGCCTTCC GCAGCTGGGC TGTACAAACC TTCCGGGCAG CCTGTGAGAC CTTTGATGTC 
CGAAGCAAAC ATCACATTCA GATCCCGAAA CGCTTCACTT CCAATGCAAC ATGGGAGCCA 
CAGCAATATA GGCTCATCCA GAGCCCGGAG CCTTTAGACC TCAACAGAGC CCTCAGCAGC 
ATCCATGCAA AGAACGTGTT TACCATGAGG CTGAAATCCC AGCAGAATCT GCAGAGTGAA 
AAGTCCAGCC GCACCACCCT CCTCGTTCAG CTCACCTTCG AGGGCAGCCG AGGGCCCAGC 
TACCTGCCTG GGGAACACCT GGGGATCTTC CCAGGCAACC AGACCGCCCT GGTGCAGGGA 
ATCTTGGAGC GAGTTGTGGA TTGTCCTACA CCACACCAAA CTGTGTGCCT GGAGGTTCTG 
GATGAGAGCG GCAGCTACTG GGTCAAAGAC AAGAGGCTGC CCCCCTGCTC ACTCAGCCAA 
GCCCTCACCT ACTTCCTGGA CATTACGACC CCTCCCACCC AGCTGCAGCT CCACAAGCTG 
GCTCGCTTTG CCACGGACGA GACGGATAGG CAGAGATTGG AGGCCTTGTG TCAGCCCTCA 
GAGTACAATG ACTGGAAGTT CAGCAACAAC CCCACGTTCC TGGAGGTGCT TGAAGAGTTC 
CCTTCCTTGC ATGTGCCCGC TGCCTTCCTG CTGTCGCAGC TCCCTATCTT GAAGCCCCGC 
TACTACTCCA TCAGCTCCTC CCAGGACCAC ACCCCCTCGG AGGTTCACCT CACTGTGGCC 
GTGGTCACCT ACCGCACCCG AGATGGTCAG GGTCCCCTGC ACCATGGTGT CTGCAGCACT 
TGGATCAGGA ACCTGAAGCC CCAGGACCCA GTGCCCTGCT TTGTGCGAAG TGTCAGTGGC 
TTCCAGCTCC CTGAGGACCC CTCCCAGCCT TGCATCCTCA TTGGGCCTGG TACGGGCATT 
GCTCCCTTCC GAAGTTTCTG GCAGCAGCGG CTCCATGACT CCCAGCACAA AGGGCTCAAA 
GGAGGCCGCA TGAGCTTGGT GTTTGGGTGC CGGCACCCGG AGGAGGACCA CCTCTATCAG 
GAAGAAATGC AGGAGATGGT CCGCAAGAGA GTGCTGTTCC AGGTGCACAC AGGCTACTCC 
CGGCTGCCCG GCAAACCCAA GGTCTACGTT CAGGACATCC TGCAAAAGCA GCTGGCCAAT 
GAGGTACTCA GCGTGCTCCA CGGGGAGCAG GGCCACCTCT ACATTTGCGG AGATGTGCGC 
ATGGCTCGGG ATGTGGCTAC CACATTGAAG AAGCTGGTGG CCACCAAGCT GAACTTGAGC 
GAGGAGCAGG TGGAAGACTA TTTCTTCCAG CTCAAGAGCC AGAAACGTTA TCATGAAGAT 
ATCTTCGGTG CAGTCTTTTC CTATGGGGCA AAAAAGGGCA GCGCCTTGGA GGAGCCCAAA 
GCCACGAGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035057.1
CDS	256..3690
Translation	MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLHVTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEFINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARNCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEFTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANMLLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSFQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPWKTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFNTKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEPQQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIFPGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKLARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDHTPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGIAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYVQDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHEDIFGAVFSYGAKKGSALEEPKATRL

Target ORF information:

RefSeq Version	NM_010927.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010927.4

ORF Insert Sequence:

ATGGCTTGCC CCTGGAAGTT TCTCTTCAAA GTCAAATCCT ACCAAAGTGA CCTGAAAGAG 
GAAAAGGACA TTAACAACAA CGTGAAGAAA ACCCCTTGTG CTGTTCTCAG CCCAACAATA 
CAAGATGACC CTAAGAGTCA CCAAAATGGC TCCCCGCAGC TCCTCACTGG GACAGCACAG 
AATGTTCCAG AATCCCTGGA CAAGCTGCAT GTGACATCGA CCCGTCCACA GTATGTGAGG 
ATCAAAAACT GGGGCAGTGG AGAGATTTTG CATGACACTC TTCACCACAA GGCCACATCG 
GATTTCACTT GCAAGTCCAA GTCTTGCTTG GGGTCCATCA TGAACCCCAA GAGTTTGACC 
AGAGGACCCA GAGACAAGCC TACCCCTCTG GAGGAGCTCC TGCCTCATGC CATTGAGTTC 
ATCAACCAGT ATTATGGCTC CTTTAAAGAG GCAAAAATAG AGGAACATCT GGCCAGGCTG 
GAAGCTGTAA CAAAGGAAAT AGAAACAACA GGAACCTACC AGCTCACTCT GGATGAGCTC 
ATCTTTGCCA CCAAGATGGC CTGGAGGAAT GCCCCTCGCT GCATCGGCAG GATCCAGTGG 
TCCAACCTGC AGGTCTTTGA CGCTCGGAAC TGTAGCACAG CACAGGAAAT GTTTCAGCAC 
ATCTGCAGAC ACATACTTTA TGCCACCAAC AATGGCAACA TCAGGTCGGC CATCACTGTG 
TTCCCCCAGC GGAGTGACGG CAAACATGAC TTCAGGCTCT GGAATTCACA GCTCATCCGG 
TACGCTGGCT ACCAGATGCC CGATGGCACC ATCAGAGGGG ATGCTGCCAC CTTGGAGTTC 
ACCCAGTTGT GCATCGACCT AGGCTGGAAG CCCCGCTATG GCCGCTTTGA TGTGCTGCCT 
CTGGTCTTGC AAGCTGATGG TCAAGATCCA GAGGTCTTTG AAATCCCTCC TGATCTTGTG 
TTGGAGGTGA CCATGGAGCA TCCCAAGTAC GAGTGGTTCC AGGAGCTCGG GTTGAAGTGG 
TATGCACTGC CTGCCGTGGC CAACATGCTA CTGGAGGTGG GTGGCCTCGA ATTCCCAGCC 
TGCCCCTTCA ATGGTTGGTA CATGGGCACC GAGATTGGAG TTCGAGACTT CTGTGACACA 
CAGCGCTACA ACATCCTGGA GGAAGTGGGC CGAAGGATGG GCCTGGAGAC CCACACACTG 
GCCTCCCTCT GGAAAGACCG GGCTGTCACG GAGATCAATG TGGCTGTGCT CCATAGTTTC 
CAGAAGCAGA ATGTGACCAT CATGGACCAC CACACAGCCT CAGAGTCCTT CATGAAGCAC 
ATGCAGAATG AGTACCGGGC CCGTGGAGGC TGCCCGGCAG ACTGGATTTG GCTGGTCCCT 
CCAGTGTCTG GGAGCATCAC CCCTGTGTTC CACCAGGAGA TGTTGAACTA TGTCCTATCT 
CCATTCTACT ACTACCAGAT CGAGCCCTGG AAGACCCACA TCTGGCAGAA TGAGAAGCTG 
AGGCCCAGGA GGAGAGAGAT CCGATTTAGA GTCTTGGTGA AAGTGGTGTT CTTTGCTTCC 
ATGCTAATGC GAAAGGTCAT GGCTTCACGG GTCAGAGCCA CAGTCCTCTT TGCTACTGAG 
ACAGGGAAGT CTGAAGCACT AGCCAGGGAC CTGGCCACCT TGTTCAGCTA CGCCTTCAAC 
ACCAAGGTTG TCTGCATGGA CCAGTATAAG GCAAGCACCT TGGAAGAGGA GCAACTACTG 
CTGGTGGTGA CAAGCACATT TGGGAATGGA GACTGTCCCA GCAATGGGCA GACTCTGAAG 
AAATCTCTGT TCATGCTTAG AGAACTCAAC CACACCTTCA GGTATGCTGT GTTTGGCCTT 
GGCTCCAGCA TGTACCCTCA GTTCTGCGCC TTTGCTCATG ACATCGACCA GAAGCTGTCC 
CACCTGGGAG CCTCTCAGCT TGCCCCAACA GGAGAAGGGG ACGAACTCAG TGGGCAGGAG 
GATGCCTTCC GCAGCTGGGC TGTACAAACC TTCCGGGCAG CCTGTGAGAC CTTTGATGTC 
CGAAGCAAAC ATCACATTCA GATCCCGAAA CGCTTCACTT CCAATGCAAC ATGGGAGCCA 
CAGCAATATA GGCTCATCCA GAGCCCGGAG CCTTTAGACC TCAACAGAGC CCTCAGCAGC 
ATCCATGCAA AGAACGTGTT TACCATGAGG CTGAAATCCC AGCAGAATCT GCAGAGTGAA 
AAGTCCAGCC GCACCACCCT CCTCGTTCAG CTCACCTTCG AGGGCAGCCG AGGGCCCAGC 
TACCTGCCTG GGGAACACCT GGGGATCTTC CCAGGCAACC AGACCGCCCT GGTGCAGGGA 
ATCTTGGAGC GAGTTGTGGA TTGTCCTACA CCACACCAAA CTGTGTGCCT GGAGGTTCTG 
GATGAGAGCG GCAGCTACTG GGTCAAAGAC AAGAGGCTGC CCCCCTGCTC ACTCAGCCAA 
GCCCTCACCT ACTTCCTGGA CATTACGACC CCTCCCACCC AGCTGCAGCT CCACAAGCTG 
GCTCGCTTTG CCACGGACGA GACGGATAGG CAGAGATTGG AGGCCTTGTG TCAGCCCTCA 
GAGTACAATG ACTGGAAGTT CAGCAACAAC CCCACGTTCC TGGAGGTGCT TGAAGAGTTC 
CCTTCCTTGC ATGTGCCCGC TGCCTTCCTG CTGTCGCAGC TCCCTATCTT GAAGCCCCGC 
TACTACTCCA TCAGCTCCTC CCAGGACCAC ACCCCCTCGG AGGTTCACCT CACTGTGGCC 
GTGGTCACCT ACCGCACCCG AGATGGTCAG GGTCCCCTGC ACCATGGTGT CTGCAGCACT 
TGGATCAGGA ACCTGAAGCC CCAGGACCCA GTGCCCTGCT TTGTGCGAAG TGTCAGTGGC 
TTCCAGCTCC CTGAGGACCC CTCCCAGCCT TGCATCCTCA TTGGGCCTGG TACGGGCATT 
GCTCCCTTCC GAAGTTTCTG GCAGCAGCGG CTCCATGACT CCCAGCACAA AGGGCTCAAA 
GGAGGCCGCA TGAGCTTGGT GTTTGGGTGC CGGCACCCGG AGGAGGACCA CCTCTATCAG 
GAAGAAATGC AGGAGATGGT CCGCAAGAGA GTGCTGTTCC AGGTGCACAC AGGCTACTCC 
CGGCTGCCCG GCAAACCCAA GGTCTACGTT CAGGACATCC TGCAAAAGCA GCTGGCCAAT 
GAGGTACTCA GCGTGCTCCA CGGGGAGCAG GGCCACCTCT ACATTTGCGG AGATGTGCGC 
ATGGCTCGGG ATGTGGCTAC CACATTGAAG AAGCTGGTGG CCACCAAGCT GAACTTGAGC 
GAGGAGCAGG TGGAAGACTA TTTCTTCCAG CTCAAGAGCC AGAAACGTTA TCATGAAGAT 
ATCTTCGGTG CAGTCTTTTC CTATGGGGCA AAAAAGGGCA GCGCCTTGGA GGAGCCCAAA 
GCCACGAGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos2 ( NM_001313921.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300850.1 Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu83579
Clone ID Related Accession (Same CDS sequence)	NM_001313921.1 , NM_001313922.1
Accession Version	NM_001313921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576684800000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, inducible isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ057491.1, M87039.1 and AL592185.35. Transcript Variant: This variant (2) contains an alternate exon in place of the first two exons compared to variant 1. This difference results in translation initiation at a downstream start codon and an isoform (b) that is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCCCA AGAGTTTGAC CAGAGGACCC AGAGACAAGC CTACCCCTCT GGAGGAGCTC 
CTGCCTCATG CCATTGAGTT CATCAACCAG TATTATGGCT CCTTTAAAGA GGCAAAAATA 
GAGGAACATC TGGCCAGGCT GGAAGCTGTA ACAAAGGAAA TAGAAACAAC AGGAACCTAC 
CAGCTCACTC TGGATGAGCT CATCTTTGCC ACCAAGATGG CCTGGAGGAA TGCCCCTCGC 
TGCATCGGCA GGATCCAGTG GTCCAACCTG CAGGTCTTTG ACGCTCGGAA CTGTAGCACA 
GCACAGGAAA TGTTTCAGCA CATCTGCAGA CACATACTTT ATGCCACCAA CAATGGCAAC 
ATCAGGTCGG CCATCACTGT GTTCCCCCAG CGGAGTGACG GCAAACATGA CTTCAGGCTC 
TGGAATTCAC AGCTCATCCG GTACGCTGGC TACCAGATGC CCGATGGCAC CATCAGAGGG 
GATGCTGCCA CCTTGGAGTT CACCCAGTTG TGCATCGACC TAGGCTGGAA GCCCCGCTAT 
GGCCGCTTTG ATGTGCTGCC TCTGGTCTTG CAAGCTGATG GTCAAGATCC AGAGGTCTTT 
GAAATCCCTC CTGATCTTGT GTTGGAGGTG ACCATGGAGC ATCCCAAGTA CGAGTGGTTC 
CAGGAGCTCG GGTTGAAGTG GTATGCACTG CCTGCCGTGG CCAACATGCT ACTGGAGGTG 
GGTGGCCTCG AATTCCCAGC CTGCCCCTTC AATGGTTGGT ACATGGGCAC CGAGATTGGA 
GTTCGAGACT TCTGTGACAC ACAGCGCTAC AACATCCTGG AGGAAGTGGG CCGAAGGATG 
GGCCTGGAGA CCCACACACT GGCCTCCCTC TGGAAAGACC GGGCTGTCAC GGAGATCAAT 
GTGGCTGTGC TCCATAGTTT CCAGAAGCAG AATGTGACCA TCATGGACCA CCACACAGCC 
TCAGAGTCCT TCATGAAGCA CATGCAGAAT GAGTACCGGG CCCGTGGAGG CTGCCCGGCA 
GACTGGATTT GGCTGGTCCC TCCAGTGTCT GGGAGCATCA CCCCTGTGTT CCACCAGGAG 
ATGTTGAACT ATGTCCTATC TCCATTCTAC TACTACCAGA TCGAGCCCTG GAAGACCCAC 
ATCTGGCAGA ATGAGAAGCT GAGGCCCAGG AGGAGAGAGA TCCGATTTAG AGTCTTGGTG 
AAAGTGGTGT TCTTTGCTTC CATGCTAATG CGAAAGGTCA TGGCTTCACG GGTCAGAGCC 
ACAGTCCTCT TTGCTACTGA GACAGGGAAG TCTGAAGCAC TAGCCAGGGA CCTGGCCACC 
TTGTTCAGCT ACGCCTTCAA CACCAAGGTT GTCTGCATGG ACCAGTATAA GGCAAGCACC 
TTGGAAGAGG AGCAACTACT GCTGGTGGTG ACAAGCACAT TTGGGAATGG AGACTGTCCC 
AGCAATGGGC AGACTCTGAA GAAATCTCTG TTCATGCTTA GAGAACTCAA CCACACCTTC 
AGGTATGCTG TGTTTGGCCT TGGCTCCAGC ATGTACCCTC AGTTCTGCGC CTTTGCTCAT 
GACATCGACC AGAAGCTGTC CCACCTGGGA GCCTCTCAGC TTGCCCCAAC AGGAGAAGGG 
GACGAACTCA GTGGGCAGGA GGATGCCTTC CGCAGCTGGG CTGTACAAAC CTTCCGGGCA 
GCCTGTGAGA CCTTTGATGT CCGAAGCAAA CATCACATTC AGATCCCGAA ACGCTTCACT 
TCCAATGCAA CATGGGAGCC ACAGCAATAT AGGCTCATCC AGAGCCCGGA GCCTTTAGAC 
CTCAACAGAG CCCTCAGCAG CATCCATGCA AAGAACGTGT TTACCATGAG GCTGAAATCC 
CAGCAGAATC TGCAGAGTGA AAAGTCCAGC CGCACCACCC TCCTCGTTCA GCTCACCTTC 
GAGGGCAGCC GAGGGCCCAG CTACCTGCCT GGGGAACACC TGGGGATCTT CCCAGGCAAC 
CAGACCGCCC TGGTGCAGGG AATCTTGGAG CGAGTTGTGG ATTGTCCTAC ACCACACCAA 
ACTGTGTGCC TGGAGGTTCT GGATGAGAGC GGCAGCTACT GGGTCAAAGA CAAGAGGCTG 
CCCCCCTGCT CACTCAGCCA AGCCCTCACC TACTTCCTGG ACATTACGAC CCCTCCCACC 
CAGCTGCAGC TCCACAAGCT GGCTCGCTTT GCCACGGACG AGACGGATAG GCAGAGATTG 
GAGGCCTTGT GTCAGCCCTC AGAGTACAAT GACTGGAAGT TCAGCAACAA CCCCACGTTC 
CTGGAGGTGC TTGAAGAGTT CCCTTCCTTG CATGTGCCCG CTGCCTTCCT GCTGTCGCAG 
CTCCCTATCT TGAAGCCCCG CTACTACTCC ATCAGCTCCT CCCAGGACCA CACCCCCTCG 
GAGGTTCACC TCACTGTGGC CGTGGTCACC TACCGCACCC GAGATGGTCA GGGTCCCCTG 
CACCATGGTG TCTGCAGCAC TTGGATCAGG AACCTGAAGC CCCAGGACCC AGTGCCCTGC 
TTTGTGCGAA GTGTCAGTGG CTTCCAGCTC CCTGAGGACC CCTCCCAGCC TTGCATCCTC 
ATTGGGCCTG GTACGGGCAT TGCTCCCTTC CGAAGTTTCT GGCAGCAGCG GCTCCATGAC 
TCCCAGCACA AAGGGCTCAA AGGAGGCCGC ATGAGCTTGG TGTTTGGGTG CCGGCACCCG 
GAGGAGGACC ACCTCTATCA GGAAGAAATG CAGGAGATGG TCCGCAAGAG AGTGCTGTTC 
CAGGTGCACA CAGGCTACTC CCGGCTGCCC GGCAAACCCA AGGTCTACGT TCAGGACATC 
CTGCAAAAGC AGCTGGCCAA TGAGGTACTC AGCGTGCTCC ACGGGGAGCA GGGCCACCTC 
TACATTTGCG GAGATGTGCG CATGGCTCGG GATGTGGCTA CCACATTGAA GAAGCTGGTG 
GCCACCAAGC TGAACTTGAG CGAGGAGCAG GTGGAAGACT ATTTCTTCCA GCTCAAGAGC 
CAGAAACGTT ATCATGAAGA TATCTTCGGT GCAGTCTTTT CCTATGGGGC AAAAAAGGGC 
AGCGCCTTGG AGGAGCCCAA AGCCACGAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300850.1
CDS	346..3441
Translation	MNPKSLTRGPRDKPTPLEELLPHAIEFINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARNCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEFTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANMLLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSFQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPWKTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFNTKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEPQQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIFPGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKLARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDHTPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGIAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYVQDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHEDIFGAVFSYGAKKGSALEEPKATRL

Target ORF information:

RefSeq Version	NM_001313921.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313921.1

ORF Insert Sequence:

ATGAACCCCA AGAGTTTGAC CAGAGGACCC AGAGACAAGC CTACCCCTCT GGAGGAGCTC 
CTGCCTCATG CCATTGAGTT CATCAACCAG TATTATGGCT CCTTTAAAGA GGCAAAAATA 
GAGGAACATC TGGCCAGGCT GGAAGCTGTA ACAAAGGAAA TAGAAACAAC AGGAACCTAC 
CAGCTCACTC TGGATGAGCT CATCTTTGCC ACCAAGATGG CCTGGAGGAA TGCCCCTCGC 
TGCATCGGCA GGATCCAGTG GTCCAACCTG CAGGTCTTTG ACGCTCGGAA CTGTAGCACA 
GCACAGGAAA TGTTTCAGCA CATCTGCAGA CACATACTTT ATGCCACCAA CAATGGCAAC 
ATCAGGTCGG CCATCACTGT GTTCCCCCAG CGGAGTGACG GCAAACATGA CTTCAGGCTC 
TGGAATTCAC AGCTCATCCG GTACGCTGGC TACCAGATGC CCGATGGCAC CATCAGAGGG 
GATGCTGCCA CCTTGGAGTT CACCCAGTTG TGCATCGACC TAGGCTGGAA GCCCCGCTAT 
GGCCGCTTTG ATGTGCTGCC TCTGGTCTTG CAAGCTGATG GTCAAGATCC AGAGGTCTTT 
GAAATCCCTC CTGATCTTGT GTTGGAGGTG ACCATGGAGC ATCCCAAGTA CGAGTGGTTC 
CAGGAGCTCG GGTTGAAGTG GTATGCACTG CCTGCCGTGG CCAACATGCT ACTGGAGGTG 
GGTGGCCTCG AATTCCCAGC CTGCCCCTTC AATGGTTGGT ACATGGGCAC CGAGATTGGA 
GTTCGAGACT TCTGTGACAC ACAGCGCTAC AACATCCTGG AGGAAGTGGG CCGAAGGATG 
GGCCTGGAGA CCCACACACT GGCCTCCCTC TGGAAAGACC GGGCTGTCAC GGAGATCAAT 
GTGGCTGTGC TCCATAGTTT CCAGAAGCAG AATGTGACCA TCATGGACCA CCACACAGCC 
TCAGAGTCCT TCATGAAGCA CATGCAGAAT GAGTACCGGG CCCGTGGAGG CTGCCCGGCA 
GACTGGATTT GGCTGGTCCC TCCAGTGTCT GGGAGCATCA CCCCTGTGTT CCACCAGGAG 
ATGTTGAACT ATGTCCTATC TCCATTCTAC TACTACCAGA TCGAGCCCTG GAAGACCCAC 
ATCTGGCAGA ATGAGAAGCT GAGGCCCAGG AGGAGAGAGA TCCGATTTAG AGTCTTGGTG 
AAAGTGGTGT TCTTTGCTTC CATGCTAATG CGAAAGGTCA TGGCTTCACG GGTCAGAGCC 
ACAGTCCTCT TTGCTACTGA GACAGGGAAG TCTGAAGCAC TAGCCAGGGA CCTGGCCACC 
TTGTTCAGCT ACGCCTTCAA CACCAAGGTT GTCTGCATGG ACCAGTATAA GGCAAGCACC 
TTGGAAGAGG AGCAACTACT GCTGGTGGTG ACAAGCACAT TTGGGAATGG AGACTGTCCC 
AGCAATGGGC AGACTCTGAA GAAATCTCTG TTCATGCTTA GAGAACTCAA CCACACCTTC 
AGGTATGCTG TGTTTGGCCT TGGCTCCAGC ATGTACCCTC AGTTCTGCGC CTTTGCTCAT 
GACATCGACC AGAAGCTGTC CCACCTGGGA GCCTCTCAGC TTGCCCCAAC AGGAGAAGGG 
GACGAACTCA GTGGGCAGGA GGATGCCTTC CGCAGCTGGG CTGTACAAAC CTTCCGGGCA 
GCCTGTGAGA CCTTTGATGT CCGAAGCAAA CATCACATTC AGATCCCGAA ACGCTTCACT 
TCCAATGCAA CATGGGAGCC ACAGCAATAT AGGCTCATCC AGAGCCCGGA GCCTTTAGAC 
CTCAACAGAG CCCTCAGCAG CATCCATGCA AAGAACGTGT TTACCATGAG GCTGAAATCC 
CAGCAGAATC TGCAGAGTGA AAAGTCCAGC CGCACCACCC TCCTCGTTCA GCTCACCTTC 
GAGGGCAGCC GAGGGCCCAG CTACCTGCCT GGGGAACACC TGGGGATCTT CCCAGGCAAC 
CAGACCGCCC TGGTGCAGGG AATCTTGGAG CGAGTTGTGG ATTGTCCTAC ACCACACCAA 
ACTGTGTGCC TGGAGGTTCT GGATGAGAGC GGCAGCTACT GGGTCAAAGA CAAGAGGCTG 
CCCCCCTGCT CACTCAGCCA AGCCCTCACC TACTTCCTGG ACATTACGAC CCCTCCCACC 
CAGCTGCAGC TCCACAAGCT GGCTCGCTTT GCCACGGACG AGACGGATAG GCAGAGATTG 
GAGGCCTTGT GTCAGCCCTC AGAGTACAAT GACTGGAAGT TCAGCAACAA CCCCACGTTC 
CTGGAGGTGC TTGAAGAGTT CCCTTCCTTG CATGTGCCCG CTGCCTTCCT GCTGTCGCAG 
CTCCCTATCT TGAAGCCCCG CTACTACTCC ATCAGCTCCT CCCAGGACCA CACCCCCTCG 
GAGGTTCACC TCACTGTGGC CGTGGTCACC TACCGCACCC GAGATGGTCA GGGTCCCCTG 
CACCATGGTG TCTGCAGCAC TTGGATCAGG AACCTGAAGC CCCAGGACCC AGTGCCCTGC 
TTTGTGCGAA GTGTCAGTGG CTTCCAGCTC CCTGAGGACC CCTCCCAGCC TTGCATCCTC 
ATTGGGCCTG GTACGGGCAT TGCTCCCTTC CGAAGTTTCT GGCAGCAGCG GCTCCATGAC 
TCCCAGCACA AAGGGCTCAA AGGAGGCCGC ATGAGCTTGG TGTTTGGGTG CCGGCACCCG 
GAGGAGGACC ACCTCTATCA GGAAGAAATG CAGGAGATGG TCCGCAAGAG AGTGCTGTTC 
CAGGTGCACA CAGGCTACTC CCGGCTGCCC GGCAAACCCA AGGTCTACGT TCAGGACATC 
CTGCAAAAGC AGCTGGCCAA TGAGGTACTC AGCGTGCTCC ACGGGGAGCA GGGCCACCTC 
TACATTTGCG GAGATGTGCG CATGGCTCGG GATGTGGCTA CCACATTGAA GAAGCTGGTG 
GCCACCAAGC TGAACTTGAG CGAGGAGCAG GTGGAAGACT ATTTCTTCCA GCTCAAGAGC 
CAGAAACGTT ATCATGAAGA TATCTTCGGT GCAGTCTTTT CCTATGGGGC AAAAAAGGGC 
AGCGCCTTGG AGGAGCCCAA AGCCACGAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos2 ( NM_001313922.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300851.1 Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu83579
Clone ID Related Accession (Same CDS sequence)	NM_001313921.1 , NM_001313922.1
Accession Version	NM_001313922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576771200000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, inducible isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN693247.1, M87039.1 and AL592185.35. Transcript Variant: This variant (3) contains an alternate exon in place of the first two exons compared to variant 1. This difference results in translation initiation at a downstream start codon and an isoform (b) that is shorter at the N-terminus compared to isoform a. Variants 2 and 3 both encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCCCA AGAGTTTGAC CAGAGGACCC AGAGACAAGC CTACCCCTCT GGAGGAGCTC 
CTGCCTCATG CCATTGAGTT CATCAACCAG TATTATGGCT CCTTTAAAGA GGCAAAAATA 
GAGGAACATC TGGCCAGGCT GGAAGCTGTA ACAAAGGAAA TAGAAACAAC AGGAACCTAC 
CAGCTCACTC TGGATGAGCT CATCTTTGCC ACCAAGATGG CCTGGAGGAA TGCCCCTCGC 
TGCATCGGCA GGATCCAGTG GTCCAACCTG CAGGTCTTTG ACGCTCGGAA CTGTAGCACA 
GCACAGGAAA TGTTTCAGCA CATCTGCAGA CACATACTTT ATGCCACCAA CAATGGCAAC 
ATCAGGTCGG CCATCACTGT GTTCCCCCAG CGGAGTGACG GCAAACATGA CTTCAGGCTC 
TGGAATTCAC AGCTCATCCG GTACGCTGGC TACCAGATGC CCGATGGCAC CATCAGAGGG 
GATGCTGCCA CCTTGGAGTT CACCCAGTTG TGCATCGACC TAGGCTGGAA GCCCCGCTAT 
GGCCGCTTTG ATGTGCTGCC TCTGGTCTTG CAAGCTGATG GTCAAGATCC AGAGGTCTTT 
GAAATCCCTC CTGATCTTGT GTTGGAGGTG ACCATGGAGC ATCCCAAGTA CGAGTGGTTC 
CAGGAGCTCG GGTTGAAGTG GTATGCACTG CCTGCCGTGG CCAACATGCT ACTGGAGGTG 
GGTGGCCTCG AATTCCCAGC CTGCCCCTTC AATGGTTGGT ACATGGGCAC CGAGATTGGA 
GTTCGAGACT TCTGTGACAC ACAGCGCTAC AACATCCTGG AGGAAGTGGG CCGAAGGATG 
GGCCTGGAGA CCCACACACT GGCCTCCCTC TGGAAAGACC GGGCTGTCAC GGAGATCAAT 
GTGGCTGTGC TCCATAGTTT CCAGAAGCAG AATGTGACCA TCATGGACCA CCACACAGCC 
TCAGAGTCCT TCATGAAGCA CATGCAGAAT GAGTACCGGG CCCGTGGAGG CTGCCCGGCA 
GACTGGATTT GGCTGGTCCC TCCAGTGTCT GGGAGCATCA CCCCTGTGTT CCACCAGGAG 
ATGTTGAACT ATGTCCTATC TCCATTCTAC TACTACCAGA TCGAGCCCTG GAAGACCCAC 
ATCTGGCAGA ATGAGAAGCT GAGGCCCAGG AGGAGAGAGA TCCGATTTAG AGTCTTGGTG 
AAAGTGGTGT TCTTTGCTTC CATGCTAATG CGAAAGGTCA TGGCTTCACG GGTCAGAGCC 
ACAGTCCTCT TTGCTACTGA GACAGGGAAG TCTGAAGCAC TAGCCAGGGA CCTGGCCACC 
TTGTTCAGCT ACGCCTTCAA CACCAAGGTT GTCTGCATGG ACCAGTATAA GGCAAGCACC 
TTGGAAGAGG AGCAACTACT GCTGGTGGTG ACAAGCACAT TTGGGAATGG AGACTGTCCC 
AGCAATGGGC AGACTCTGAA GAAATCTCTG TTCATGCTTA GAGAACTCAA CCACACCTTC 
AGGTATGCTG TGTTTGGCCT TGGCTCCAGC ATGTACCCTC AGTTCTGCGC CTTTGCTCAT 
GACATCGACC AGAAGCTGTC CCACCTGGGA GCCTCTCAGC TTGCCCCAAC AGGAGAAGGG 
GACGAACTCA GTGGGCAGGA GGATGCCTTC CGCAGCTGGG CTGTACAAAC CTTCCGGGCA 
GCCTGTGAGA CCTTTGATGT CCGAAGCAAA CATCACATTC AGATCCCGAA ACGCTTCACT 
TCCAATGCAA CATGGGAGCC ACAGCAATAT AGGCTCATCC AGAGCCCGGA GCCTTTAGAC 
CTCAACAGAG CCCTCAGCAG CATCCATGCA AAGAACGTGT TTACCATGAG GCTGAAATCC 
CAGCAGAATC TGCAGAGTGA AAAGTCCAGC CGCACCACCC TCCTCGTTCA GCTCACCTTC 
GAGGGCAGCC GAGGGCCCAG CTACCTGCCT GGGGAACACC TGGGGATCTT CCCAGGCAAC 
CAGACCGCCC TGGTGCAGGG AATCTTGGAG CGAGTTGTGG ATTGTCCTAC ACCACACCAA 
ACTGTGTGCC TGGAGGTTCT GGATGAGAGC GGCAGCTACT GGGTCAAAGA CAAGAGGCTG 
CCCCCCTGCT CACTCAGCCA AGCCCTCACC TACTTCCTGG ACATTACGAC CCCTCCCACC 
CAGCTGCAGC TCCACAAGCT GGCTCGCTTT GCCACGGACG AGACGGATAG GCAGAGATTG 
GAGGCCTTGT GTCAGCCCTC AGAGTACAAT GACTGGAAGT TCAGCAACAA CCCCACGTTC 
CTGGAGGTGC TTGAAGAGTT CCCTTCCTTG CATGTGCCCG CTGCCTTCCT GCTGTCGCAG 
CTCCCTATCT TGAAGCCCCG CTACTACTCC ATCAGCTCCT CCCAGGACCA CACCCCCTCG 
GAGGTTCACC TCACTGTGGC CGTGGTCACC TACCGCACCC GAGATGGTCA GGGTCCCCTG 
CACCATGGTG TCTGCAGCAC TTGGATCAGG AACCTGAAGC CCCAGGACCC AGTGCCCTGC 
TTTGTGCGAA GTGTCAGTGG CTTCCAGCTC CCTGAGGACC CCTCCCAGCC TTGCATCCTC 
ATTGGGCCTG GTACGGGCAT TGCTCCCTTC CGAAGTTTCT GGCAGCAGCG GCTCCATGAC 
TCCCAGCACA AAGGGCTCAA AGGAGGCCGC ATGAGCTTGG TGTTTGGGTG CCGGCACCCG 
GAGGAGGACC ACCTCTATCA GGAAGAAATG CAGGAGATGG TCCGCAAGAG AGTGCTGTTC 
CAGGTGCACA CAGGCTACTC CCGGCTGCCC GGCAAACCCA AGGTCTACGT TCAGGACATC 
CTGCAAAAGC AGCTGGCCAA TGAGGTACTC AGCGTGCTCC ACGGGGAGCA GGGCCACCTC 
TACATTTGCG GAGATGTGCG CATGGCTCGG GATGTGGCTA CCACATTGAA GAAGCTGGTG 
GCCACCAAGC TGAACTTGAG CGAGGAGCAG GTGGAAGACT ATTTCTTCCA GCTCAAGAGC 
CAGAAACGTT ATCATGAAGA TATCTTCGGT GCAGTCTTTT CCTATGGGGC AAAAAAGGGC 
AGCGCCTTGG AGGAGCCCAA AGCCACGAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300851.1
CDS	246..3341
Translation	MNPKSLTRGPRDKPTPLEELLPHAIEFINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARNCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEFTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANMLLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSFQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPWKTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFNTKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEPQQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIFPGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKLARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDHTPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGIAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYVQDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHEDIFGAVFSYGAKKGSALEEPKATRL

Target ORF information:

RefSeq Version	NM_001313922.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313922.1

ORF Insert Sequence:

ATGAACCCCA AGAGTTTGAC CAGAGGACCC AGAGACAAGC CTACCCCTCT GGAGGAGCTC 
CTGCCTCATG CCATTGAGTT CATCAACCAG TATTATGGCT CCTTTAAAGA GGCAAAAATA 
GAGGAACATC TGGCCAGGCT GGAAGCTGTA ACAAAGGAAA TAGAAACAAC AGGAACCTAC 
CAGCTCACTC TGGATGAGCT CATCTTTGCC ACCAAGATGG CCTGGAGGAA TGCCCCTCGC 
TGCATCGGCA GGATCCAGTG GTCCAACCTG CAGGTCTTTG ACGCTCGGAA CTGTAGCACA 
GCACAGGAAA TGTTTCAGCA CATCTGCAGA CACATACTTT ATGCCACCAA CAATGGCAAC 
ATCAGGTCGG CCATCACTGT GTTCCCCCAG CGGAGTGACG GCAAACATGA CTTCAGGCTC 
TGGAATTCAC AGCTCATCCG GTACGCTGGC TACCAGATGC CCGATGGCAC CATCAGAGGG 
GATGCTGCCA CCTTGGAGTT CACCCAGTTG TGCATCGACC TAGGCTGGAA GCCCCGCTAT 
GGCCGCTTTG ATGTGCTGCC TCTGGTCTTG CAAGCTGATG GTCAAGATCC AGAGGTCTTT 
GAAATCCCTC CTGATCTTGT GTTGGAGGTG ACCATGGAGC ATCCCAAGTA CGAGTGGTTC 
CAGGAGCTCG GGTTGAAGTG GTATGCACTG CCTGCCGTGG CCAACATGCT ACTGGAGGTG 
GGTGGCCTCG AATTCCCAGC CTGCCCCTTC AATGGTTGGT ACATGGGCAC CGAGATTGGA 
GTTCGAGACT TCTGTGACAC ACAGCGCTAC AACATCCTGG AGGAAGTGGG CCGAAGGATG 
GGCCTGGAGA CCCACACACT GGCCTCCCTC TGGAAAGACC GGGCTGTCAC GGAGATCAAT 
GTGGCTGTGC TCCATAGTTT CCAGAAGCAG AATGTGACCA TCATGGACCA CCACACAGCC 
TCAGAGTCCT TCATGAAGCA CATGCAGAAT GAGTACCGGG CCCGTGGAGG CTGCCCGGCA 
GACTGGATTT GGCTGGTCCC TCCAGTGTCT GGGAGCATCA CCCCTGTGTT CCACCAGGAG 
ATGTTGAACT ATGTCCTATC TCCATTCTAC TACTACCAGA TCGAGCCCTG GAAGACCCAC 
ATCTGGCAGA ATGAGAAGCT GAGGCCCAGG AGGAGAGAGA TCCGATTTAG AGTCTTGGTG 
AAAGTGGTGT TCTTTGCTTC CATGCTAATG CGAAAGGTCA TGGCTTCACG GGTCAGAGCC 
ACAGTCCTCT TTGCTACTGA GACAGGGAAG TCTGAAGCAC TAGCCAGGGA CCTGGCCACC 
TTGTTCAGCT ACGCCTTCAA CACCAAGGTT GTCTGCATGG ACCAGTATAA GGCAAGCACC 
TTGGAAGAGG AGCAACTACT GCTGGTGGTG ACAAGCACAT TTGGGAATGG AGACTGTCCC 
AGCAATGGGC AGACTCTGAA GAAATCTCTG TTCATGCTTA GAGAACTCAA CCACACCTTC 
AGGTATGCTG TGTTTGGCCT TGGCTCCAGC ATGTACCCTC AGTTCTGCGC CTTTGCTCAT 
GACATCGACC AGAAGCTGTC CCACCTGGGA GCCTCTCAGC TTGCCCCAAC AGGAGAAGGG 
GACGAACTCA GTGGGCAGGA GGATGCCTTC CGCAGCTGGG CTGTACAAAC CTTCCGGGCA 
GCCTGTGAGA CCTTTGATGT CCGAAGCAAA CATCACATTC AGATCCCGAA ACGCTTCACT 
TCCAATGCAA CATGGGAGCC ACAGCAATAT AGGCTCATCC AGAGCCCGGA GCCTTTAGAC 
CTCAACAGAG CCCTCAGCAG CATCCATGCA AAGAACGTGT TTACCATGAG GCTGAAATCC 
CAGCAGAATC TGCAGAGTGA AAAGTCCAGC CGCACCACCC TCCTCGTTCA GCTCACCTTC 
GAGGGCAGCC GAGGGCCCAG CTACCTGCCT GGGGAACACC TGGGGATCTT CCCAGGCAAC 
CAGACCGCCC TGGTGCAGGG AATCTTGGAG CGAGTTGTGG ATTGTCCTAC ACCACACCAA 
ACTGTGTGCC TGGAGGTTCT GGATGAGAGC GGCAGCTACT GGGTCAAAGA CAAGAGGCTG 
CCCCCCTGCT CACTCAGCCA AGCCCTCACC TACTTCCTGG ACATTACGAC CCCTCCCACC 
CAGCTGCAGC TCCACAAGCT GGCTCGCTTT GCCACGGACG AGACGGATAG GCAGAGATTG 
GAGGCCTTGT GTCAGCCCTC AGAGTACAAT GACTGGAAGT TCAGCAACAA CCCCACGTTC 
CTGGAGGTGC TTGAAGAGTT CCCTTCCTTG CATGTGCCCG CTGCCTTCCT GCTGTCGCAG 
CTCCCTATCT TGAAGCCCCG CTACTACTCC ATCAGCTCCT CCCAGGACCA CACCCCCTCG 
GAGGTTCACC TCACTGTGGC CGTGGTCACC TACCGCACCC GAGATGGTCA GGGTCCCCTG 
CACCATGGTG TCTGCAGCAC TTGGATCAGG AACCTGAAGC CCCAGGACCC AGTGCCCTGC 
TTTGTGCGAA GTGTCAGTGG CTTCCAGCTC CCTGAGGACC CCTCCCAGCC TTGCATCCTC 
ATTGGGCCTG GTACGGGCAT TGCTCCCTTC CGAAGTTTCT GGCAGCAGCG GCTCCATGAC 
TCCCAGCACA AAGGGCTCAA AGGAGGCCGC ATGAGCTTGG TGTTTGGGTG CCGGCACCCG 
GAGGAGGACC ACCTCTATCA GGAAGAAATG CAGGAGATGG TCCGCAAGAG AGTGCTGTTC 
CAGGTGCACA CAGGCTACTC CCGGCTGCCC GGCAAACCCA AGGTCTACGT TCAGGACATC 
CTGCAAAAGC AGCTGGCCAA TGAGGTACTC AGCGTGCTCC ACGGGGAGCA GGGCCACCTC 
TACATTTGCG GAGATGTGCG CATGGCTCGG GATGTGGCTA CCACATTGAA GAAGCTGGTG 
GCCACCAAGC TGAACTTGAG CGAGGAGCAG GTGGAAGACT ATTTCTTCCA GCTCAAGAGC 
CAGAAACGTT ATCATGAAGA TATCTTCGGT GCAGTCTTTT CCTATGGGGC AAAAAAGGGC 
AGCGCCTTGG AGGAGCCCAA AGCCACGAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos2 ( XM_006532446.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006532509.1 Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant X1, mRNA.

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CloneID	OMu45711
Clone ID Related Accession (Same CDS sequence)	XM_006532446.3
Accession Version	XM_006532446.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, inducible isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006532446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGAGG GGCTGAGCAG CGCTTGCGAC GCCGCCCGGC CGCGGCTCTG TCGCTTCCTG 
CGCAGGATCT TCGGCCCGAA GGTCGCCAGT CGTGTGGGCC CAGCCAGCCC AACAATACAA 
GATGACCCTA AGAGTCACCA AAATGGCTCC CCGCAGCTCC TCACTGGGAC AGCACAGAAT 
GTTCCAGAAT CCCTGGACAA GCTGCATGTG ACATCGACCC GTCCACAGTA TGTGAGGATC 
AAAAACTGGG GCAGTGGAGA GATTTTGCAT GACACTCTTC ACCACAAGGC CACATCGGAT 
TTCACTTGCA AGTCCAAGTC TTGCTTGGGG TCCATCATGA ACCCCAAGAG TTTGACCAGA 
GGACCCAGAG ACAAGCCTAC CCCTCTGGAG GAGCTCCTGC CTCATGCCAT TGAGTTCATC 
AACCAGTATT ATGGCTCCTT TAAAGAGGCA AAAATAGAGG AACATCTGGC CAGGCTGGAA 
GCTGTAACAA AGGAAATAGA AACAACAGGA ACCTACCAGC TCACTCTGGA TGAGCTCATC 
TTTGCCACCA AGATGGCCTG GAGGAATGCC CCTCGCTGCA TCGGCAGGAT CCAGTGGTCC 
AACCTGCAGG TCTTTGACGC TCGGAACTGT AGCACAGCAC AGGAAATGTT TCAGCACATC 
TGCAGACACA TACTTTATGC CACCAACAAT GGCAACATCA GGTCGGCCAT CACTGTGTTC 
CCCCAGCGGA GTGACGGCAA ACATGACTTC AGGCTCTGGA ATTCACAGCT CATCCGGTAC 
GCTGGCTACC AGATGCCCGA TGGCACCATC AGAGGGGATG CTGCCACCTT GGAGTTCACC 
CAGTTGTGCA TCGACCTAGG CTGGAAGCCC CGCTATGGCC GCTTTGATGT GCTGCCTCTG 
GTCTTGCAAG CTGATGGTCA AGATCCAGAG GTCTTTGAAA TCCCTCCTGA TCTTGTGTTG 
GAGGTGACCA TGGAGCATCC CAAGTACGAG TGGTTCCAGG AGCTCGGGTT GAAGTGGTAT 
GCACTGCCTG CCGTGGCCAA CATGCTACTG GAGGTGGGTG GCCTCGAATT CCCAGCCTGC 
CCCTTCAATG GTTGGTACAT GGGCACCGAG ATTGGAGTTC GAGACTTCTG TGACACACAG 
CGCTACAACA TCCTGGAGGA AGTGGGCCGA AGGATGGGCC TGGAGACCCA CACACTGGCC 
TCCCTCTGGA AAGACCGGGC TGTCACGGAG ATCAATGTGG CTGTGCTCCA TAGTTTCCAG 
AAGCAGAATG TGACCATCAT GGACCACCAC ACAGCCTCAG AGTCCTTCAT GAAGCACATG 
CAGAATGAGT ACCGGGCCCG TGGAGGCTGC CCGGCAGACT GGATTTGGCT GGTCCCTCCA 
GTGTCTGGGA GCATCACCCC TGTGTTCCAC CAGGAGATGT TGAACTATGT CCTATCTCCA 
TTCTACTACT ACCAGATCGA GCCCTGGAAG ACCCACATCT GGCAGAATGA GAAGCTGAGG 
CCCAGGAGGA GAGAGATCCG ATTTAGAGTC TTGGTGAAAG TGGTGTTCTT TGCTTCCATG 
CTAATGCGAA AGGTCATGGC TTCACGGGTC AGAGCCACAG TCCTCTTTGC TACTGAGACA 
GGGAAGTCTG AAGCACTAGC CAGGGACCTG GCCACCTTGT TCAGCTACGC CTTCAACACC 
AAGGTTGTCT GCATGGACCA GTATAAGGCA AGCACCTTGG AAGAGGAGCA ACTACTGCTG 
GTGGTGACAA GCACATTTGG GAATGGAGAC TGTCCCAGCA ATGGGCAGAC TCTGAAGAAA 
TCTCTGTTCA TGCTTAGAGA ACTCAACCAC ACCTTCAGGT ATGCTGTGTT TGGCCTTGGC 
TCCAGCATGT ACCCTCAGTT CTGCGCCTTT GCTCATGACA TCGACCAGAA GCTGTCCCAC 
CTGGGAGCCT CTCAGCTTGC CCCAACAGGA GAAGGGGACG AACTCAGTGG GCAGGAGGAT 
GCCTTCCGCA GCTGGGCTGT ACAAACCTTC CGGGCAGCCT GTGAGACCTT TGATGTCCGA 
AGCAAACATC ACATTCAGAT CCCGAAACGC TTCACTTCCA ATGCAACATG GGAGCCACAG 
CAATATAGGC TCATCCAGAG CCCGGAGCCT TTAGACCTCA ACAGAGCCCT CAGCAGCATC 
CATGCAAAGA ACGTGTTTAC CATGAGGCTG AAATCCCAGC AGAATCTGCA GAGTGAAAAG 
TCCAGCCGCA CCACCCTCCT CGTTCAGCTC ACCTTCGAGG GCAGCCGAGG GCCCAGCTAC 
CTGCCTGGGG AACACCTGGG GATCTTCCCA GGCAACCAGA CCGCCCTGGT GCAGGGAATC 
TTGGAGCGAG TTGTGGATTG TCCTACACCA CACCAAACTG TGTGCCTGGA GGTTCTGGAT 
GAGAGCGGCA GCTACTGGGT CAAAGACAAG AGGCTGCCCC CCTGCTCACT CAGCCAAGCC 
CTCACCTACT TCCTGGACAT TACGACCCCT CCCACCCAGC TGCAGCTCCA CAAGCTGGCT 
CGCTTTGCCA CGGACGAGAC GGATAGGCAG AGATTGGAGG CCTTGTGTCA GCCCTCAGAG 
TACAATGACT GGAAGTTCAG CAACAACCCC ACGTTCCTGG AGGTGCTTGA AGAGTTCCCT 
TCCTTGCATG TGCCCGCTGC CTTCCTGCTG TCGCAGCTCC CTATCTTGAA GCCCCGCTAC 
TACTCCATCA GCTCCTCCCA GGACCACACC CCCTCGGAGG TTCACCTCAC TGTGGCCGTG 
GTCACCTACC GCACCCGAGA TGGTCAGGGT CCCCTGCACC ATGGTGTCTG CAGCACTTGG 
ATCAGGAACC TGAAGCCCCA GGACCCAGTG CCCTGCTTTG TGCGAAGTGT CAGTGGCTTC 
CAGCTCCCTG AGGACCCCTC CCAGCCTTGC ATCCTCATTG GGCCTGGTAC GGGCATTGCT 
CCCTTCCGAA GTTTCTGGCA GCAGCGGCTC CATGACTCCC AGCACAAAGG GCTCAAAGGA 
GGCCGCATGA GCTTGGTGTT TGGGTGCCGG CACCCGGAGG AGGACCACCT CTATCAGGAA 
GAAATGCAGG AGATGGTCCG CAAGAGAGTG CTGTTCCAGG TGCACACAGG CTACTCCCGG 
CTGCCCGGCA AACCCAAGGT CTACGTTCAG GACATCCTGC AAAAGCAGCT GGCCAATGAG 
GTACTCAGCG TGCTCCACGG GGAGCAGGGC CACCTCTACA TTTGCGGAGA TGTGCGCATG 
GCTCGGGATG TGGCTACCAC ATTGAAGAAG CTGGTGGCCA CCAAGCTGAA CTTGAGCGAG 
GAGCAGGTGG AAGACTATTT CTTCCAGCTC AAGAGCCAGA AACGTTATCA TGAAGATATC 
TTCGGTGCAG TCTTTTCCTA TGGGGCAAAA AAGGGCAGCG CCTTGGAGGA GCCCAAAGCC 
ACGAGGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006532509.1
CDS	108..3539
Translation	MREGLSSACDAARPRLCRFLRRIFGPKVASRVGPASPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLHVTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEFINQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARNCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEFTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANMLLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSFQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPWKTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFNTKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEPQQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIFPGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKLARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDHTPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGIAPFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYVQDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHEDIFGAVFSYGAKKGSALEEPKATRL

Target ORF information:

RefSeq Version	XM_006532446.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 2, inducible (Nos2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006532446.3

ORF Insert Sequence:

ATGCGCGAGG GGCTGAGCAG CGCTTGCGAC GCCGCCCGGC CGCGGCTCTG TCGCTTCCTG 
CGCAGGATCT TCGGCCCGAA GGTCGCCAGT CGTGTGGGCC CAGCCAGCCC AACAATACAA 
GATGACCCTA AGAGTCACCA AAATGGCTCC CCGCAGCTCC TCACTGGGAC AGCACAGAAT 
GTTCCAGAAT CCCTGGACAA GCTGCATGTG ACATCGACCC GTCCACAGTA TGTGAGGATC 
AAAAACTGGG GCAGTGGAGA GATTTTGCAT GACACTCTTC ACCACAAGGC CACATCGGAT 
TTCACTTGCA AGTCCAAGTC TTGCTTGGGG TCCATCATGA ACCCCAAGAG TTTGACCAGA 
GGACCCAGAG ACAAGCCTAC CCCTCTGGAG GAGCTCCTGC CTCATGCCAT TGAGTTCATC 
AACCAGTATT ATGGCTCCTT TAAAGAGGCA AAAATAGAGG AACATCTGGC CAGGCTGGAA 
GCTGTAACAA AGGAAATAGA AACAACAGGA ACCTACCAGC TCACTCTGGA TGAGCTCATC 
TTTGCCACCA AGATGGCCTG GAGGAATGCC CCTCGCTGCA TCGGCAGGAT CCAGTGGTCC 
AACCTGCAGG TCTTTGACGC TCGGAACTGT AGCACAGCAC AGGAAATGTT TCAGCACATC 
TGCAGACACA TACTTTATGC CACCAACAAT GGCAACATCA GGTCGGCCAT CACTGTGTTC 
CCCCAGCGGA GTGACGGCAA ACATGACTTC AGGCTCTGGA ATTCACAGCT CATCCGGTAC 
GCTGGCTACC AGATGCCCGA TGGCACCATC AGAGGGGATG CTGCCACCTT GGAGTTCACC 
CAGTTGTGCA TCGACCTAGG CTGGAAGCCC CGCTATGGCC GCTTTGATGT GCTGCCTCTG 
GTCTTGCAAG CTGATGGTCA AGATCCAGAG GTCTTTGAAA TCCCTCCTGA TCTTGTGTTG 
GAGGTGACCA TGGAGCATCC CAAGTACGAG TGGTTCCAGG AGCTCGGGTT GAAGTGGTAT 
GCACTGCCTG CCGTGGCCAA CATGCTACTG GAGGTGGGTG GCCTCGAATT CCCAGCCTGC 
CCCTTCAATG GTTGGTACAT GGGCACCGAG ATTGGAGTTC GAGACTTCTG TGACACACAG 
CGCTACAACA TCCTGGAGGA AGTGGGCCGA AGGATGGGCC TGGAGACCCA CACACTGGCC 
TCCCTCTGGA AAGACCGGGC TGTCACGGAG ATCAATGTGG CTGTGCTCCA TAGTTTCCAG 
AAGCAGAATG TGACCATCAT GGACCACCAC ACAGCCTCAG AGTCCTTCAT GAAGCACATG 
CAGAATGAGT ACCGGGCCCG TGGAGGCTGC CCGGCAGACT GGATTTGGCT GGTCCCTCCA 
GTGTCTGGGA GCATCACCCC TGTGTTCCAC CAGGAGATGT TGAACTATGT CCTATCTCCA 
TTCTACTACT ACCAGATCGA GCCCTGGAAG ACCCACATCT GGCAGAATGA GAAGCTGAGG 
CCCAGGAGGA GAGAGATCCG ATTTAGAGTC TTGGTGAAAG TGGTGTTCTT TGCTTCCATG 
CTAATGCGAA AGGTCATGGC TTCACGGGTC AGAGCCACAG TCCTCTTTGC TACTGAGACA 
GGGAAGTCTG AAGCACTAGC CAGGGACCTG GCCACCTTGT TCAGCTACGC CTTCAACACC 
AAGGTTGTCT GCATGGACCA GTATAAGGCA AGCACCTTGG AAGAGGAGCA ACTACTGCTG 
GTGGTGACAA GCACATTTGG GAATGGAGAC TGTCCCAGCA ATGGGCAGAC TCTGAAGAAA 
TCTCTGTTCA TGCTTAGAGA ACTCAACCAC ACCTTCAGGT ATGCTGTGTT TGGCCTTGGC 
TCCAGCATGT ACCCTCAGTT CTGCGCCTTT GCTCATGACA TCGACCAGAA GCTGTCCCAC 
CTGGGAGCCT CTCAGCTTGC CCCAACAGGA GAAGGGGACG AACTCAGTGG GCAGGAGGAT 
GCCTTCCGCA GCTGGGCTGT ACAAACCTTC CGGGCAGCCT GTGAGACCTT TGATGTCCGA 
AGCAAACATC ACATTCAGAT CCCGAAACGC TTCACTTCCA ATGCAACATG GGAGCCACAG 
CAATATAGGC TCATCCAGAG CCCGGAGCCT TTAGACCTCA ACAGAGCCCT CAGCAGCATC 
CATGCAAAGA ACGTGTTTAC CATGAGGCTG AAATCCCAGC AGAATCTGCA GAGTGAAAAG 
TCCAGCCGCA CCACCCTCCT CGTTCAGCTC ACCTTCGAGG GCAGCCGAGG GCCCAGCTAC 
CTGCCTGGGG AACACCTGGG GATCTTCCCA GGCAACCAGA CCGCCCTGGT GCAGGGAATC 
TTGGAGCGAG TTGTGGATTG TCCTACACCA CACCAAACTG TGTGCCTGGA GGTTCTGGAT 
GAGAGCGGCA GCTACTGGGT CAAAGACAAG AGGCTGCCCC CCTGCTCACT CAGCCAAGCC 
CTCACCTACT TCCTGGACAT TACGACCCCT CCCACCCAGC TGCAGCTCCA CAAGCTGGCT 
CGCTTTGCCA CGGACGAGAC GGATAGGCAG AGATTGGAGG CCTTGTGTCA GCCCTCAGAG 
TACAATGACT GGAAGTTCAG CAACAACCCC ACGTTCCTGG AGGTGCTTGA AGAGTTCCCT 
TCCTTGCATG TGCCCGCTGC CTTCCTGCTG TCGCAGCTCC CTATCTTGAA GCCCCGCTAC 
TACTCCATCA GCTCCTCCCA GGACCACACC CCCTCGGAGG TTCACCTCAC TGTGGCCGTG 
GTCACCTACC GCACCCGAGA TGGTCAGGGT CCCCTGCACC ATGGTGTCTG CAGCACTTGG 
ATCAGGAACC TGAAGCCCCA GGACCCAGTG CCCTGCTTTG TGCGAAGTGT CAGTGGCTTC 
CAGCTCCCTG AGGACCCCTC CCAGCCTTGC ATCCTCATTG GGCCTGGTAC GGGCATTGCT 
CCCTTCCGAA GTTTCTGGCA GCAGCGGCTC CATGACTCCC AGCACAAAGG GCTCAAAGGA 
GGCCGCATGA GCTTGGTGTT TGGGTGCCGG CACCCGGAGG AGGACCACCT CTATCAGGAA 
GAAATGCAGG AGATGGTCCG CAAGAGAGTG CTGTTCCAGG TGCACACAGG CTACTCCCGG 
CTGCCCGGCA AACCCAAGGT CTACGTTCAG GACATCCTGC AAAAGCAGCT GGCCAATGAG 
GTACTCAGCG TGCTCCACGG GGAGCAGGGC CACCTCTACA TTTGCGGAGA TGTGCGCATG 
GCTCGGGATG TGGCTACCAC ATTGAAGAAG CTGGTGGCCA CCAAGCTGAA CTTGAGCGAG 
GAGCAGGTGG AAGACTATTT CTTCCAGCTC AAGAGCCAGA AACGTTATCA TGAAGATATC 
TTCGGTGCAG TCTTTTCCTA TGGGGCAAAA AAGGGCAGCG CCTTGGAGGA GCCCAAAGCC 
ACGAGGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Brucella abortus nitric oxide metabolite regulates inflammasome activation and IL-1β secretion in murine macrophages.
European journal of immunology49(7)1023-1037(2019 Jul)
Campos PC,Gomes MTR,Marinho FAV,Guimarães ES,de Moura Lodi Cruz MGF,Oliveira SC

IFN-γ-dependent nitric oxide suppresses Brucella-induced arthritis by inhibition of inflammasome activation.
Journal of leukocyte biology106(1)27-34(2019 Jul)
Lacey CA,Chambers CA,Mitchell WJ,Skyberg JA

Mechanism and regulation of ferrous heme-nitric oxide (NO) oxidation in NO synthases.
The Journal of biological chemistry294(19)7904-7916(2019 05)
Tejero J,Hunt AP,Santolini J,Lehnert N,Stuehr DJ

Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive- and anxiety-like behaviour, and gut microbiome composition.
Scientific reports9(1)6456(2019 Apr)
Inserra A,Choo JM,Lewis MD,Rogers GB,Wong ML,Licinio J

Transcriptional Regulation of Nos2 via STAT5B Binding to Nos2 Gene Promoter Mediates Nitric Oxide Production: Relevance in β-Cell Maintenance.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology52(1)141-155(2019)
Joseph A,Nair LCR,Johnson BS,Thomas PL,Padmanabhan RA,Puthumadathil N,Laloraya M

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Nos2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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