GenCRISPR gRNA & HDR Template Design Tools

Accelerate Precision Gene Editing

Resources » Bioinformatics Tools » GenCRISPR gRNA Design Tool

The GenCRISPR gRNA Design Tools makes guide RNA and HDR template design easy – Simply select your experiment type to get started.

Start Your Project

Knockout HDR Knock-in Prime Editing

Note: Selecting HDR knock-in will enable HDR template design options

Why choose our CRISPR design tools?

Supports 1-1500bp deletions, 1bp-20kb insertions
Linear ssDNA / linear closed-end dsDNA / circular dsDNA formats available
CTS option and updated on-target and off-target scores increase editing efficiency
Multiple sequence check function helps avoid mistakes
Design Tool User Guide

Comprehensive Functions: Simply enter your desired gene symbol or sequence for guided support from design to ordering.
More Editing Approaches: Supports knock-ins, knockouts, and sequence replacement, with knock-ins up to 100 nt.
Enhances Editing Efficiency: Includes nicking sgRNA, 3' extension, and silent mutations.
Easy to Use: Displays base and amino acid changes, with multiple indices for easy selection.
Design Tool User Guide

Easily design compatible
HDR templates & sgRNAs

Automatically optimize
homology arm design

Select from ssDNA
or dsDNA formats

Create an SNP or insert
any sequence up to 20 kb

Streamline your HDR
template & sgRNA orders

sgRNA/template pairs are
ranked by algorithm scores

Start Your Project

Knockout HDR Knock-in

Note: Selecting HDR knock-in will enable HDR template design options

Genome-wide gRNA databases for CRISPR editing and transcription activation
Pre-validated gRNA sequences design by the Broad Institute Ready for delivery; Full-Genome Coverage