mRNA QC Services

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Send Us Your mRNA Samples for Quality Control Analysis

Ensure the quality of your mRNA by leveraging GenScript’s mRNA manufacturing expertise to perform reliable QC analysis on your IVT mRNA samples.

Simply fill out the form to request a quote for your preferred QC tests and send us your mRNA sample. Analysis typically takes 1-3 weeks depending on the specific tests (see table below).

mRNA QC Service Introduction

Option	mRNA QC Test	TAT, days	Amount Required	Method
1	PolyA Tail Length Assay	7	300pmol	RNase digestion and purification to recover the polyA tail fragment, followed by polyA tail length analysis by small RNA bio-analyzer kit assay or LC-MS.
2	Capping Efficiency Assay	14-21	300pmol	RNase digestion to isolate a short oligo from the 5’ end of the mRNA sample to be analyzed by LC-MS to calculate the capping efficiency.
3	dsRNA Residue Assay	10	40ug	Slot-blot assay using a dsRNA-specific antibody to test for dsRNA residues in mRNA sample.
4	RNA Capillary Electrophoresis	5	10ug	Bioanalyzer assay to test RNA length and purity.
5	Total Protein Residue Assay	7	200ug	Nano-orange assay to test the residual protein amount in mRNA sample.
6	Plasmid DNA Residue Assay	14	15ug	q-PCR assay to test the residual plasmid DNA residue amount in mRNA sample.
7	Quantitative Endotoxin Assay	5	15uL	GenScript ToxinSensor™ Chromogenic LAL Endotoxin Assay to test endotoxin level in mRNA sample.
8	BioBurden Assay	14	250uL	TSA plate to recover a broad range of microorganisms such as aerobic and anaerobic bacteria from liquid.
9	Sanger Sequencing	7	5ug	RT-PCR and sanger sequencing, alignment with target sequence for data analysis. 2-4X coverage.
10	NGS	14	5ug	Illumina platform with 1G data amount, 10,000X depth.
11	RNA Aggregation Assay	7	30ug	SEC-HPLC method