Targeted LNP Formulation

Targeted LNP Service

Ligand-Decorated LNPs Conjugation Route for Targeted Delivery

Precisely deliver mRNA where it matters most. Our antibody- and peptide-decorated LNPs use optimized chemistries to achieve targeted cellular delivery with high efficiency and reproducibility.

T-cell targeted LNP: CD3-Ab/LNP

CD3 is widely expressed on T cells and is key to TCR signaling. Linking anti-CD3 antibodies to LNPs boosts mRNA delivery to primary T cells and enhances expression in the spleen.

Brain targeted LNP: APOE-pep/LNP

Site specific conjugation of peptide ligand onto LNP significantly improved targeted delivery efficiency to cells.

APOE-LNP/EGFP mRNA to U87 Cells

This Click Reaction platform is optimized for site specific conjugation with peptide ligand for LNP decoration.

Quality Control and Specification

Case Studies

• T Cell Targeted LNP: CD3-Ab/LNP
  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD3 Antibody  (Clone: OKT3, mAb, Mouse, GenScript, A02199), 100ng of mRNA encapsulated in LNP were incubated with jurkat cells in RPMI140 +10%FBS at 37C for 24 hrs. The expression of Fluc was detected using Luciferase Assay (Promega, E4030).

  

  eGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD3 Antibody  (Clone OKT3, mAb, Mouse, GenScript), 10-150ng of mRNA encapsulated in LNP were incubated with primary T cells  for 24 hrs. The expression of eGFP was detected using flow cytometry. W1, W2 and W3 indicate different wash conditions.

  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-mouse CD3 Antibody, 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

• CD34+ Cell Targeted LNP: CD34-Ab/LNP
  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with anti-Human CD34 Antibody (Purified anti-human CD34 antibody(Anti-Human CD34 Antibody, mAb, Mouse, GenScript, CP0001) 100ng of mRNA encapsulated in LNP were incubated with Kasumi-1 cells  for 24 hrs. The expression of eGFP was detected using flow cytometry.

  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with ALC0315-LNP or ALC0315-LNP decorated with anti-Human CD34 Antibody (Purified anti-human CD34 antibody(Anti-Human CD34 Antibody, mAb, Mouse, GenScript, CP0001) 100ng of mRNA encapsulated in LNP were incubated with primary human CD34+ cells  for 24 hrs. The expression of eGFP was detected using flow cytometry.

• Liver Targeted LNP: Tri-GalNac/LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

  

  EGFP mRNA(N1-methyl-pseU) was encapsulated with SM102-LNP or SM102-LNP decorated with TriGalNac, 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and liver organs were lysed. The eGFP expression in lysate was detected using western blot method.

• Liver Targeted LNP: 20DODAP LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

• Spleen Targeted LNP: SM10218PA LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.

• Lung Targeted LNP: 50DOTAP LNP
  

  

  Fluc mRNA(N1-methyl-pseU) was encapsulated with LNP , 0.3mg/kg of mRNA encapsulated in LNP were injection through tail vein into mice, after 24 hrs, mice were sacrificed, and the expression of Fluc mRNA was measured with whole body imaging with different organs.