Cas9 ELISA Kit

Key Features

Broad Recognition

of different variants and NLS
(nuclear localization signals)

Ultra Sensitive

To as low as 0.125 ng/ml
(10x lower than alternative providers)

Highly Specific

No non-specific binding and eliminated background signal

Highly Accurate

Consistent recovery between 80-120% for various sample amount

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Assay Principle

• Cas9 ELISA Kit
  

  Figure 1. Schematic diagram of Cas9 ELISA kit detection principle

Validation Data

• Broad Recognition
  

  Figure 2. Different Cas9 variants and NLS types/numbers/positions can be detected by Cas9 ELISA Kit (Cat.No. L01069).

• Excellent Dilution Linearity
  

  Figure 3. Three batches of Cas9 ELISA kits (Cat.No. L01069) were used to test samples with different dilution factors to evaluate the recovery rate. The result showed that the recovery rates of different batches of kits are not affected by the dilution factor.

• High Recovery

  The Cas9 ELISA kit (Cat.No. L01069) was used to test different CAR-T samples with different spike concentration (Table 1). The results showed that the kit achieved recovery rate between 80%-120% in all samples (Figure 4), demonstrating its accuracy and repeatability across a range of sample conditions

  Sample ID	Theoretical Spike Conc. (ng/mL)	Measured Spike Conc. (ng/mL)	Spike Recovery Rate
  Sample 1	0.250	0.240	96%
  Sample 2	0.829	0.712	87%
  Sample 3	1.000	1.071	107%
  Sample 4	1.228	1.222	99%
  Sample 5	1.273	1.108	87%
  Sample 6	1.419	1.324	93%
  Sample 7	1.804	1.689	94%
  Sample 8	2.272	2.273	100%
  Sample 9	2.379	2.230	94%
  Sample 10	4.000	3.848	96%

  Table 1

  

  Figure 4

• High Accuracy

  Three different batches of Cas9 ELISA kit were used to test three different samples with variant concentration levels (Table 2). The results showed that the intra-batch and inter-batch CV are both less than 10%, and accuracy above 95% across all conditions (Figure 5).

  Theoretical Conc. (ng/mL)	Intra-assay (n=10)			Inter-assay (n=30)
  	Average Measured Conc. (ng/mL)	CV	Accuracy	Average Measured Conc. (ng/mL)	CV	Accuracy
  6	5.813	5.3%	97%	5.863	7.0%	98%
  1.5	1.503	4.1%	100%	1.471	6.6%	98%
  0.375	0.383	4.9%	102%	0.376	6.8%	100%

  Table 2

  

  Figure 5

Resources

Cas9 ELISA Kit Flyer

Free Download

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