CRISPR Resources

CRISPR Services

CRISPR gRNA Plasmids

Licensed from the Feng Zhang Laboratory at the Broad Institute

Learn More

CRISPR
sgRNA

A powerful tool for creating gene knock-ins and knock-outs

Learn More

HDR Knock-in Templates

ssDNA and dsDNA HDR donor templates for effective gene knock-in

Learn More

CRISPR Cell Lines

Genetically modified cell line in any mammalian cell line and targeting any gene.

Learn More

Microbial Gene Editing

Highly efficient, seamless genome editing in E.coli using CRISPR technology

Learn More

CRISPR gRNA Libraries

Genome-wide gRNA libraries for efficient screening

Learn More

Home » Broad sgRNA Database

Broad Institute sgRNA Databases

gRNA designed on-demand for any DNA sequence you input
Algorithm developed and validated by Feng Zhang lab, Broad Institute

Quote/Order Request Now

Overview

Home » Broad sgRNA Database

CRISPR Resources

Overview

Genome-wide gRNA databases for CRISPR genome editing and transcription activation

To facilitate your research using CRISPR technology, GenScript provides genome-wide databases containing pre-validated gRNA sequences design by the Broad Institute of Harvard and MIT. If you want to view all, please click gRNA list.

Reference: Sanjana et al., Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.

Genome Editing

Transcription Activation

Ready for delivery

More than 20,000 gRNA constructs are in-stock and ready for delivery at $99/clone.

Full-Genome Coverage

GenScript's gRNA Database contains 6 unique, pre-validated gRNA sequences targeting each of the 19,050 genes in the human genome and 20,611 genes in the mouse genome. SpCas9 gRNA sequences are targeted to constitutive exons and designed for minimal off-target effects.
GenScript's SAM Database contains 6 unique, pre-validated gRNA sequences targeting each of the 19,050 genes in the human genome. SAM gRNA sequences target the first 200bp upstream of each transcription start site.