Idiotypes are antigenic determinants located in the variable region of antibodies. An anti-idiotype antibody can specifically bind to an idiotype of another antibody, usually an antibody drug including monoclonal antibody, bispecific antibody (BsAb), antibody-drug conjugates(ADC), etc. Hence, anti-idiotype antibodies can be used in preclinical practices for pharmacokinetic (PK) analysis to measure antibody drug levels. Since an anti-idiotype antibody is very similar to an anti-drug antibody (ADA), anti-idiotype antibodies can also be used as positive controls for immunogenicity assays.
GenScript has developed a broad range of off-the-shelf anti-idiotype antibodies against blockbuster drugs to help accelerate your research of biopharmaceuticals.
Anti-Abatacept/Orencia (Target: CTLA-4-Fc)
| Cat. No. | Product Name | Binding Type | Recommend Applications | Recommended Pairs (Capture-Detection) | Order |
|---|---|---|---|---|---|
| A02262-40 | Anti-Abatacept Antibody (CT.F3), mAb, Mouse | - | ELISA, PK study |
A02262-40 (C) - A02263-40 (D) A02263-40 (C) - A02262-40 (D) |
|
| A02263-40 | Anti-Abatacept Antibody (CT.E8), mAb, Mouse | - | ELISA, PK study |
|
GenScript’s anti-idiotype antibodies are suitable for multiple application formats in PK studies.
GenScript provides two types of monoclonal anti-idiotype antibodies used for PK studies to quantify free drug or total drug level.
| Type | 1. Antigen-blocking | 2. Non-blocking |
|---|---|---|
| Schematic diagram |  |
 |
| Feature | Paratope-specific | Not Paratope-specific |
| Antigen binding | Inhibitory | Not inhibitory |
| Application | Detect free drug only | Detect total drug (free and bound) |
GenScript’s anti-idiotype antibodies are suitable for multiple application formats in ADA studies.
BLI (Biolayer interferometry) binding affinity measurements of MonoRab™ Anti-Evolocumab Antibody (39B8), mAb, Rabbit (GenScript, A02293) to Evolocumab.Evolocumab captured on HFC (Anti-Human IgG Fc) Probes can bind MonoRab™ Anti-Evolocumab Antibody (39B8), mAb, Rabbit with a dissociation constant (KD) of less than 1 pM.
Specificity analysis of the detection of Ocrelizumab.
Ocrelizumab samples at 5 concentrations were spiked with 10 μg/mL of Rituximab. The test result demonstrated that the high concentration of Rituximab did not interfere with the detection of Ocrelizumab.
In this ELISA assay, MonoRab™ Anti-Ocrelizumab Antibody (12G10), mAb, Rabbit (GenScript, A02258) was coated at a concentration of 1 μg/mL, and MonoRab™ Anti-Ocrelizumab Antibody (15E7), mAb, Rabbit (GenScript, A02259) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL.
ELISA binding of Anti-Trastuzumab Antibody (11C4), mAb, Mouse (GenScript, A02032-40) with Trastuzumab and Pertuzumab. It shows the antibody does not recognize Pertuzumab.
Coating antigen: Pertuzumab 1 µg/ml. Trastuzumab 1 µg/ml.
Anti-Trastuzumab Antibody (11C4), mAb, Mouse (GenScript, A02032-40) dilution start from 1,000 ng/ml.
MRD Analysis of the Detection of Lecanemab in Biological Matrix.
The MRD is the minimum dilution necessary for the detection of Lecanemab in biological matrix with least interference. Serum samples from cynomolgus monkey were serially diluted to determine the MRD of this assay, and the test result suggested that MRD was as 1:10.
In this ELISA assay, MonoRab™ Anti-Lecanemab Antibody (44G1), mAb, Rabbit (GenScript, A02270) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Lecanemab Antibody (4F2), mAb, Rabbit (GenScript, A02271) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL.
Standard Curve of Lecanemab Sandwich ELISA by Anti-Lecanemab idiotype antibody 44G1 and 4F2.
In this Sandwich ELISA assay, MonoRab™ Anti-Lecanemab Antibody (44G1), mAb, Rabbit (GenScript, A02270) was coated at a concentration of 1 μg/ml, and MonoRab™ Anti-Lecanemab Antibody (4F2), mAb, Rabbit (GenScript, A02271) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL. GenScript can provide customized conjugation service for this product per customer's request.
In this assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square equal to 0.99971. The typicaldynamic range of the assay is 0.31 - 20 ng/mL and its detection limit is 0.31 ng/mL.
Standard curve of the detection of Abatacept by Anti-Abatacept Antibody (CT.E8) and anti-human IgG Fc antibody.
In the ELISA assay shown in this chart, Anti-Abatacept Antibody (CT.E8), mAb, Mouse (GenScript, A02263) was coated at a concentration of 1 μg/ml, and Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) was used as a detection antibody at a dilution of 1:2000.
In this assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square is greater than 0.99. The typicaldynamic range of the assay is 4.69-300 ng/mL and its sensitivity of detecting Abatacept is up to 4.69 ng/mL.
Standard curve and specificity analysis of the detection of Certolizumab pegol by Anti-Certolizumab Antibody (12A3) and anti-PEG antibody.
In the ELISA assay shown in this chart, THE™ PEG Antibody, mAb, Mouse (GenScript, A01795) was coated at a concentration of 2 μg/mL, and MonoRab™ Anti-Certolizumab Antibody (12A3), mAb, Rabbit (GenScript, A02269) conjugated with Biotin was used as a detection antibody at a concentration of 0.5 μg/mL. In this assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square is greater than 0.999. The typicaldynamic range of the assay is 0.55-400 ng/mL and its sensitivity of detecting Certolizumab pegol is up to 0.55 ng/mL.
Anti-Zolbetuximab Antibody (34E11) or (9C9) inhibits the binding of Zolbetuximab to Claudin18.2 (CLDN18.2).
Anti-Zolbetuximab Antibody (34E11), mAb, Mouse (GenScript, A02260) exhibited non-inhibitory properties, whereas Anti-Zolbetuximab Antibody (9C9), mAb, Mouse (GenScript, A02261) demonstrated significant inhibitory properties with an IC50 value of 0.44 µg/mL.
Standard curve of the detection of Trastuzumab deruxtecan (DS-8201) by Anti-Trastuzumab Antibody (15H2) and DXd Antibody (39F4)
In this ELISA assay, Anti-Trastuzumab Antibody (11C4), mAb, Mouse (GenScript, A02032) was coated at a concentration of 2 μg/mL, and DXd Antibody (39F4), mAb, Mouse (GenScript, A02217) conjugated with HRP was used as a detection antibody at a concentration of 0.22 μg/mL.
In this assay, a four-parameter logistic curve fitting program was used to create a standard curve with the R-Square is greater than 0.99. The typicaldynamic range of the assay is 1.56 - 100 ng/mL and its sensitivity of detecting Trastuzumab deruxtecan (DS-8201) is up to 1.56 ng/mL.
Guaranteed application in immunoassay development for PK and ADA analysis
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Learn MoreAnti-polypeptide Antibody
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Anti-Human IgG Antibody
| Cat. No. | Product Name | Quantity | Price | Order |
|---|---|---|---|---|
|
A01854
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Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb |
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$105.00 200 μl |
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A01855
|
Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb |
|
$105.00 200 μl |
|
Anti-ADC Payload Antibody
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Anti-PEG Antibody
| Cat. No. | Product Name | Quantity | Price | Order |
|---|---|---|---|---|
|
A01795
|
THE™ PEG Antibody, mAb, Mouse |
|
$144.00 |
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A01796
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THE™ PEG Antibody [Biotin], mAb, Mouse |
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$158.00 100 μg |
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