Anti-idiotype antibody service

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Overview

An anti-idiotype antibody (anti-ID Ab, see the schematic diagram) binds to the idiotype of another antibody, usually an antibody-drug. An idiotype can be defined as the specific combination of idiotopes present within an antibody complementary determining regions. Since anti-ID Abs are capable of binding to antibody-drugs within biological fluids, they are commonly used in the preclinical setting to analyze the pharmacokinetics (PK) and pharmacodynamics (PD) of antibody-drugs. Due to the similarity between anti-ID Abs and anti-drug antibodies, anti-ID Abs are also commonly used as a reference standard for antibody-drug immunogenicity, anti-drug antibody (ADA) studies, and neutralization antibody measurements.

Leveraging its extensive expertise in antibody development, GenScript partners with investigators just like you in developing highly sensitive and specific polyclonal and monoclonal anti-ID antibodies to best suit your particular downstream applications.

Highlights

Fast Turnaround Time

7 weeks to deliver purified Anti-ID pAb
9 weeks to deliver purified Anti-ID mAb

High-quality Delivery

Antibody purity > 90%
ELISA > 729K
Cross-reaction < 2%

Extensive Expertise

> 20 years of global service experience
> 90% success rate of anti-ID projects

Antibody Services

Types of Anti-idiotype Antibodies

Type	1. Antigen-blocking	2. Non-blocking	3. Complex-specific
Schematic Diagram
Feature	Paratope-specific	Not Paratope-specific	Partially paratope-specific
Antigen Binding	Inhibitory	Not inhibitory	Not inhibitory
Application	Detect free drug only	Detect total drug (free and bound)	Detect bound drug typically
Selection Guideline	Type 1 or type 2 could be applied to detect antibody drug when having a cell-bound drug target e.g. PD1/PDL1 Type 1 & type 2 may both be needed when having a secret drug target e.g. TNF

Services Details

Rabbit Anti-ID Monoclonal Antibody Package

Anti-ID Rabbit Monoclonal Antibody Package

Customized Rabbit Anti-ID Monoclonal Antibody Package
Target Type	Full length IgG/ADC/Bi-specific Ab	scFv/VHH	RNA/VLP/(Neo)peptide /recombinant protein/small molecule/Cell etc.
Phase I Immunogen Preparation	F(ab’) 2 preparation or whole antibody/ADC	Whole scFv/VHH	KLH/BSA conjugation or whole protein/cell
Phase II Animal Immunization	Conventional immunization protocol Express immunization protocol (MonoExpress™) ^Preferred
Phase III B Cell Cloning and Screening	Indirect ELISA Blocking ELISA^*
Phase IV Sequencing & rAb Production	5 clones sequencing reports Guaranteed 0.1mg recombinant antibodies production and ELISA positive validation (With/without blocker & non-blocker)
TAT	Starting from 9 weeks

^* Required for blocking & non-blocker anti-ID screening

Rabbit Anti-ID Polyclonal Antibody Package

Guaranteed Rabbit Anti-ID Polyclonal Antibody Package
Guaranteed Amount	5/10/15/20 mg >20 mg, please request a quote
Target Type^*	Full-length IgG/ADC Bi-specific Ab	scFv/VHH
Phase I Immunogen Preparation	F(ab’) 2 preparation or whole antibody/ADC	scFv/VHH
Phase II Animal Immunization	Express immunization protocol (MonoExpress™) with rabbits [OLAW&AAALAC-accredited facility]	Express immunization protocol (MonoExpress™) with rabbits [OLAW&AAALAC-accredited facility]
Phase III Purification & QC	Two-step/three-step purification	Two-step purification
TAT	Starting from 7 weeks

^* GenScript is also experienced in RNA, VLP, (Neo)peptide, recombinant protein, small molecule, cell and other target types. For more details, welcome to contact us at antibody@genscript.com.

Mouse Anti-ID Monoclonal Antibody Package

Anti-ID Mouse Monoclonal Antibody Package

Customized Mouse Anti-ID Monoclonal Antibody Package
Target Type		Full length IgG	scFv/VHH/RNA/VLP/(Neo)peptide/recombinant protein/small molecule/Cell etc.	ADC/Bi-specific Ab
Phase I Immunogen Preparation		F(ab’) 2 preparation	KLH conjugation	Fab preparation/KLH conjugation
Phase II Animal Immunization		Conventional immunization protocol Express immunization protocol (MonoExpress™) ^Preferred
Phase III Cell Fusion and Screening		Cell fusion & one round of subcloning Deliver supernatants of 20 clones, 2ml/clone Confirm the type of anti-ID mAb
Phase IV Antibody Production	Option 1 Sequencing & rAb production ^Preferred	5 clones sequencing by NGS Guaranteed 0.1mg recombinant production and ELISA validation
Phase IV Antibody Production	Option 2 Clone delivery & Ab hybridoma production	Subcloning for up to 5 clones Clone storage (Optional) Ab hybridoma production and purification
TAT		Starting from 13 weeks

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Case Studies

Rabbit Anti-ID Monoclonal Antibody

Case 1

Project Target: Tri-specific antibody

Immunogen: scFv-His

Goal: 4 Blocking & 1 non-blocking Anti-ID Abs

Process:

Results: Generate more positive clones in rabbit!

Fig1. Indirect ELISA Results of Antiserum After the 3rd Immunization

Fig2. Inhibition Results of Purified Anti-ID mAb

Case 2

Project Target: Develop anti-MMAE antibodies not recognizing MMAF through B cell cloning
Immunization Target: MMAE

Counter Screening: MMAE

Process:

Results:

Fig1. ELISA Results of 15 Developed Clones

GenScript is experienced in developing anti-ID with different kinds of target antigens.
Rabbit Anti-ID Polyclonal Antibody

Project Target: Generate rabbit pAb specific to siRNA

Immunogen: siRNA-KLH (provided by customer)

Animal Amount: 4 rabbits

Challenge: Conjugation and purification efficiency

Process:

Results:

Fig1. Purified ADA Titer
Mouse Anti-ID Monoclonal Antibody

Project Target: Screening mouse anti-ID antibody for blocking application

Immunogen: F(ab’) 2 digested by Pepsin

Immunization Protocol: Mouse express immunization protocol (MonoExpress™)

Process:

Result: 20 clones were selected for blocking application

Fig1. Inhibition Results of Purified Clone 1 - 17