Goal: Generate mouse monoclonal antibodies that are specific to claudin 18.2
Immunogen: Protein Claudin 18.2
Screening Material: Claudin 18.2 overexpressing cell line & animal tumor tissue
Results: Successfully generate 111 hCCR9-positive clones and 51 validated recombinant antibodies clones (TAT < 45 days)
After cell enrichment & cell fusion
111 ELISA positive parental clones
74 /111 unique paired antibody NGS sequencing results
51 /74 validated recombinant antibodies. The recombination positive rate of NGS sequences is 69% (51/74).
NGS sequencing results
VH Phylogenetic tree analysis, out of 111, 23 Antibodies share same sequences
VL Phylogenetic tree analysis, out of 111, 37 antibodies share same sequences
Germline Analysis and CDR Length Analysis
