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![Custom pAb Services]( "Custom pAb Services")
Overview
GenScript's standard polyclonal antibody services packages use traditional immunization protocols and allow custom polyclonal antibody and antiserum production in rabbits, mice, and rats. Multiple antibody purification options are available and include: affinity purification, protein A/G and IgY purification or just antiserum. The availability of large animal species allows polyclonal antibody and antiserum production in large quantities and choice of animal host grants the use of unrelated animal species to ensure the specificity of your polyclonal antibody.
Highlights
Record Fast
Turn-Around-Times
Higher Purified
pAb Yields!
Market High ELISA
Titers of 1:256,000
Advanced Antigen
Design ToolUnsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.
Service Details
Custom Rabbit Polyclonal Antibody Services
(SC2046) Rabbit pAb
Custom Mouse Polyclonal Antibody Services
(SC2044) Mouse pAb
Custom Rat Polyclonal Antibody Services
(SC2044) Rat pAbThese services generate custom rabbit polyclonal antibodies using peptide antigens designed and optimized using our OptimumAntigen Design Tool.
Standard Protocol (Two animals)
Steps Time from Project Initiation Time from Immunization Activity Description Peptide synthesis ≈2 weeks Day 21 - Peptide conjugation to carrier ≈3 weeks Day 7 - Primary immunization ≈4 weeks Day 0 0.2 mg per rabbit peptide-KLH conjugate, Freud's Complete Adjuvant, Subcutaneous administration 1st boost ≈6 weeks Day 14 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration 2nd boost ≈8 weeks Day 28 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration Production bleed ≈9 weeks Day 35 10-20 ml serum per rabbit 3rd boost ≈10 weeks Day 42 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration Production bleed ≈11 weeks Day 49 40 ml serum per rabbit ELISA, purification, and shipping ≈13 weeks Day 63 Extend or terminate These services generate custom mouse polyclonal antibodies using peptide antigens designed and optimized using our OptimumAntigen Design Tool.
Standard Protocol (Five animals)
Steps Time from Project Initiation Time from Immunization Activity Description Peptide synthesis ≈2 weeks Day 21 - Peptide conjugation to carrier ≈3 weeks Day 7 - Primary immunization ≈4 weeks Day 0 50 μg per mouse peptide-KLH conjugate, Freud's Complete Adjuvant, Intrapreitoneal injection. 1st boost ≈6 weeks Day 14 25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection. 2nd boost ≈8 weeks Day 28 25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection. Test bleed and ELISA ≈9 weeks Day 35 0.01-0.02 ml serum per mouse. ELISA and response evaluation. 3rd boost ≈10 weeks Day 42 25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection. Production bleed ≈11 weeks Day 49 0.5 ml serum per mouse. ELISA, purification, and shipping ≈13 weeks Day 63 Extend or terminate Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.
*During the development of the antibody, if there is the death of the experimental animal, we will choose the adjusted immunization process for re-immunization to ensure the high quality and efficiency of the order.These services generate custom rat polyclonal antibodies using peptide antigens designed and optimized using our OptimumAntigen Design Tool. Both rat antibody packages come with a guaranteed ELISA titer of ≥1:256,000.
Standard Protocol (Three animals)
Steps Time from Project Initiation Time from Immunization Activity Description Peptide synthesis ≈2 weeks Day 21 - Peptide conjugation to carrier ≈3 weeks Day 7 - Primary immunization ≈4 weeks Day 0 50 µg per rat peptide-KLH conjugate, -Freunds's Complete Adjuvant, Intraperitoneal injection 1st boost ≈6 weeks Day 14 25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection 2nd boost ≈8 weeks Day 28 25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection Test bleed and ELISA ≈9 weeks Day 35 0.01-0.02 ml serum per rat. ELISA and initial response evaluation. 3rd boost ≈10 weeks Day 42 25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection Production bleed ≈11 weeks Day 49 1.5 – 2 ml serum per rat ELISA, purification, and shipping ≈13 weeks Day 63 Extend or terminate Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.
During the development of the antibody, if there is the death of the experimental animal, we will choose the adjusted immunization process for re-immunization to ensure the high quality and efficiency of the order.Customer pAb Case Study
Learn how polyclonal antibodies maximize your chances of detecting novel target proteins
Get in Touch
with GenScript Custom
Antibody Services
Quotation and Ordering
Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.
Fill out the online form and email to antibody@genscript.comA Technical Account Manager will reply you within 24 hours to finalize the quoteTo place order, provide credit card/PO informationProduction of your project begins immediatelyA Project Manager is in contact with you during the production process to give updates -
