THE™ Anti-c-Myc-Tag mAb

Product Details

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Examples

• Sensitivity Detection
  

  

  Sensitivity analysis of THE™ c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.
  
  A: 0.2 μg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
  B: 0.1 μg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
  C: 0.05 μg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)
  
  The signal was developed with IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG

• Reactivity Test
  

  Western blot discovers working concentration comparison of THE™ c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with Mouse Anti-c-Myc mAb (9E10), showing GenScript A00704 posses excellent performance in WB assay with surprising antibody dilution.
  
  Both c-Myc antibodies starting dilution are from 1:1 000 (equivalent to 1.0 μg/ml) in assay.
  
  The signal was developed with IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

• Immunoprecipitation Application
  

  Lane 1-4: 0.5, 0.2, 0.1, and 0.05 μg Multiple Tag Cell Lysate (GenScript, M0100) as western blot control
  Lane 5-7: 1, 0.5, and 0.25 μg
  
  THE™ cMyc tag antibody (Mouse) (GenScript, A00704) with 20 μg Multiple Tag Cell Lysate (GenScript, M0100) for immunoprecipitation analysis performed by One-Step IP-Western Kit (Mouse)(GenScript, L00232)

• Immunofluorescence Staining
  

  Immunofluorescence analysis of 293 cells transfected with c-Myc tag protein using THE™ cMyc tag antibody (Cat. No. A00704).