nos2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nos2
Entrez Gene ID	100494698
Full Name	nitric oxide synthase 2
Synonyms	nos2a
General protein information	Preferred Names nitric oxide synthase 2 Names nitric oxide synthase, inducible inducible nitric oxide synthase nitric oxide synthase 2, inducible
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018091491.1	XP_017946980.1	nitric oxide synthase, inducible isoform X2
XM_002935296.5	XP_002935342.2	nitric oxide synthase, inducible
XM_002935296.4	XP_002935342.2	nitric oxide synthase, inducible isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NOS2	NP_001070267.1
Canis lupus familiaris (dog)	NOS2	XP_005624903.1
Xenopus tropicalis (tropical clawed frog)	nos2	XP_002935342.2
Macaca mulatta (Rhesus monkey)	NOS2	XP_001106245.1
Rattus norvegicus (Norway rat)	Nos2	NP_036743.3
Danio rerio (zebrafish)	nos2a	NP_001098407.1
Homo sapiens (human)	NOS2	NP_000616.3
Rattus norvegicus (Norway rat)	LOC497963	XP_006247011.1
Gallus gallus (chicken)	NOS2	NP_990292.1
Pan troglodytes (chimpanzee)	NOS2	XP_001148238.1
Mus musculus (house mouse)	Nos2	NP_035057.1
Danio rerio (zebrafish)	nos2b	NP_001106973.1

Gene Ontology

The following nos2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nos2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa05725	XM_002935296.4 Latest version!	Xenopus tropicalis nitric oxide synthase 2 (nos2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa42693	XM_018091491.1 Latest version!	Xenopus tropicalis nitric oxide synthase 2 (nos2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa52170	XM_002935296.5 Latest version!	Xenopus tropicalis nitric oxide synthase 2 (nos2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nos2 ( XM_002935296.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935342.2 Xenopus tropicalis nitric oxide synthase 2 (nos2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa05725
Clone ID Related Accession (Same CDS sequence)	XM_002935296.4
Accession Version	XM_002935296.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nitric oxide synthase, inducible isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935296.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TTAAAGAGAA AGCATTTTGC 
AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC 
ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA 
TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA 
TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA 
GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG 
GTGTATATAA GAGGCCCGAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA 
ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT 
AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA 
GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA 
ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG 
TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT 
ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA 
CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC 
ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG 
ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA 
GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC 
CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG 
TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT 
TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA 
CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG 
TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT 
ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG 
CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC 
ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT 
GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA 
TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT 
GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC 
GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA 
AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG 
AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT 
TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA 
AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT 
GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA 
TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC 
TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT 
CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG 
CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG 
GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA 
GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG 
GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT 
TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA 
AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC 
AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA 
CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT 
CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC 
CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT 
GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG 
AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA 
GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG 
AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT 
GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC 
ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG 
AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC 
GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG 
CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA CAAATTAAGA 
TATCACGAAG ATATATTTGG GCCTGCTCAT AGGCCTCAGC AGTCTACAAA TGGATACCCT 
TCTCCTGTGG CTTTTAGTAC ACCAGTCTAT ACTCCACACC ATAGAATTAC AAATATGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935342.2
CDS	310..3792
Translation	MMCPWKRFMKIGTVKEKAFCNEMDINNNLHKDFEKKHSTIIKEANNFNGTKQTMAELKSVSEKRFPENITHPQKRETCPKYLKLKNYESGSILQDTLHLKAKKNLSCQSKICQGSIMNPKVYIRGPRNEPVPQDELLPQAIEFVNQYYSSFKEPQIENHLNRLETVAKEIECTGTYQLTRDELAYATKMAWRNAPRCIGRIQWSNLQVFDARDCKTAKEMFELLCRHITYSSNGGNIRSAITIFPQRTDGKHDFRVWNSQLIRYAGFQMPDGSIVGDPASIEFTELCIHLGWKPKYSRFEILPLVLQANGQDPELFEIPLDIIDEVPIEHPKYEWFKELSLKWYTLPAVANMLLEVGGLEFSACPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRLGLETHKLASLWKDQAVIELNIAVLYSFQKHNVTIMDHHSAAESFMKHLENEYRLRGGCPADWVWLVPPMSGSITPVFHQEMLNYILSPFYYYQIDAWKTHVWQDENRKPKKKEFKFSVVAKSVLFASSLLKKTVAARTKVTILYATETGKSETFAKKLQALFSYAFNAKVLCMDDYNIRNLEDESLLLVVTSTFGNGDCPGNGEKFKKAMFSCKYLKNKLRYAVFGLGSSMYPQFCAFAHTVDQRLAQLGAAQISLTGEGDELSGQEESFLNWAVQTFKAACELFKIRDRRNIHLPKCYTSTESWSAENYRLVNDNQQYDHIKALCKMHSRSIVPMKLKFRKNLQSPKSSRTTLLVKLSYENSQEVRFMPGEHAGLFAGNQPELVASVISHLKDAPPCNQHVRLETRNEQESFWTISEKIPPCSLTQALTYFLDITTPPTQLLLKKLSLLATKVTDKSRLEELSKDADEYKQWKNHNFPNFLEVLEEFPSLQVPTTFVLTQLPPLKPRYYSISSSRDMVPGEIHLTVAVVNYTTRGGQGPLHHGVCSTWLNDLDINETVPCFIRSTNTFHLPENPSVPCVLIGPGTGISPFRGFWQQRLYDLEKKGIKAGSMILLFGCQQSGMDEIYADETQYMKSKGVLKDVYTAYSRQPGQAKVYVQDILIKNLESEVCRILLEEEGHLYVCGNFEMAKNVAQTLKSMIAKRLKLNEEEAENYFSELKNKLRYHEDIFGPAHRPQQSTNGYPSPVAFSTPVYTPHHRITNMK

Target ORF information:

RefSeq Version	XM_002935296.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nitric oxide synthase 2 (nos2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935296.4

ORF Insert Sequence:

ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TTAAAGAGAA AGCATTTTGC 
AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC 
ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA 
TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA 
TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA 
GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG 
GTGTATATAA GAGGCCCGAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA 
ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT 
AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA 
GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA 
ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG 
TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT 
ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA 
CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC 
ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG 
ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA 
GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC 
CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG 
TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT 
TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA 
CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG 
TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT 
ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG 
CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC 
ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT 
GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA 
TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT 
GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC 
GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA 
AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG 
AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT 
TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA 
AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT 
GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA 
TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC 
TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT 
CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG 
CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG 
GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA 
GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG 
GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT 
TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA 
AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC 
AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA 
CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT 
CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC 
CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT 
GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG 
AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA 
GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG 
AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT 
GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC 
ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG 
AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC 
GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG 
CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA CAAATTAAGA 
TATCACGAAG ATATATTTGG GCCTGCTCAT AGGCCTCAGC AGTCTACAAA TGGATACCCT 
TCTCCTGTGG CTTTTAGTAC ACCAGTCTAT ACTCCACACC ATAGAATTAC AAATATGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos2 ( XM_018091491.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946980.1 Xenopus tropicalis nitric oxide synthase 2 (nos2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa42693
Clone ID Related Accession (Same CDS sequence)	XM_018091491.1
Accession Version	XM_018091491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nitric oxide synthase, inducible isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TTAAAGAGAA AGCATTTTGC 
AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC 
ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA 
TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA 
TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA 
GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG 
GTGTATATAA GAGGCCCGAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA 
ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT 
AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA 
GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA 
ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG 
TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT 
ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA 
CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC 
ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG 
ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA 
GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC 
CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG 
TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT 
TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA 
CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG 
TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT 
ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG 
CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC 
ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT 
GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA 
TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT 
GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC 
GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA 
AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG 
AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT 
TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA 
AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT 
GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA 
TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC 
TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT 
CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG 
CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG 
GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA 
GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG 
GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT 
TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA 
AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC 
AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA 
CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT 
CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC 
CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT 
GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG 
AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA 
GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG 
AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT 
GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC 
ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG 
AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC 
GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG 
CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946980.1
CDS	310..3666
Translation	MMCPWKRFMKIGTVKEKAFCNEMDINNNLHKDFEKKHSTIIKEANNFNGTKQTMAELKSVSEKRFPENITHPQKRETCPKYLKLKNYESGSILQDTLHLKAKKNLSCQSKICQGSIMNPKVYIRGPRNEPVPQDELLPQAIEFVNQYYSSFKEPQIENHLNRLETVAKEIECTGTYQLTRDELAYATKMAWRNAPRCIGRIQWSNLQVFDARDCKTAKEMFELLCRHITYSSNGGNIRSAITIFPQRTDGKHDFRVWNSQLIRYAGFQMPDGSIVGDPASIEFTELCIHLGWKPKYSRFEILPLVLQANGQDPELFEIPLDIIDEVPIEHPKYEWFKELSLKWYTLPAVANMLLEVGGLEFSACPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRLGLETHKLASLWKDQAVIELNIAVLYSFQKHNVTIMDHHSAAESFMKHLENEYRLRGGCPADWVWLVPPMSGSITPVFHQEMLNYILSPFYYYQIDAWKTHVWQDENRKPKKKEFKFSVVAKSVLFASSLLKKTVAARTKVTILYATETGKSETFAKKLQALFSYAFNAKVLCMDDYNIRNLEDESLLLVVTSTFGNGDCPGNGEKFKKAMFSCKYLKNKLRYAVFGLGSSMYPQFCAFAHTVDQRLAQLGAAQISLTGEGDELSGQEESFLNWAVQTFKAACELFKIRDRRNIHLPKCYTSTESWSAENYRLVNDNQQYDHIKALCKMHSRSIVPMKLKFRKNLQSPKSSRTTLLVKLSYENSQEVRFMPGEHAGLFAGNQPELVASVISHLKDAPPCNQHVRLETRNEQESFWTISEKIPPCSLTQALTYFLDITTPPTQLLLKKLSLLATKVTDKSRLEELSKDADEYKQWKNHNFPNFLEVLEEFPSLQVPTTFVLTQLPPLKPRYYSISSSRDMVPGEIHLTVAVVNYTTRGGQGPLHHGVCSTWLNDLDINETVPCFIRSTNTFHLPENPSVPCVLIGPGTGISPFRGFWQQRLYDLEKKGIKAGSMILLFGCQQSGMDEIYADETQYMKSKGVLKDVYTAYSRQPGQAKVYVQDILIKNLESEVCRILLEEEGHLYVCGNFEMAKNVAQTLKSMIAKRLKLNEEEAENYFSELKNA

Target ORF information:

RefSeq Version	XM_018091491.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nitric oxide synthase 2 (nos2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091491.1

ORF Insert Sequence:

ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TTAAAGAGAA AGCATTTTGC 
AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC 
ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA 
TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA 
TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA 
GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG 
GTGTATATAA GAGGCCCGAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA 
ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT 
AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA 
GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA 
ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG 
TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT 
ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA 
CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC 
ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG 
ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA 
GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC 
CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG 
TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT 
TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA 
CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG 
TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT 
ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG 
CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC 
ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT 
GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA 
TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT 
GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC 
GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA 
AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG 
AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT 
TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA 
AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT 
GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA 
TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC 
TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT 
CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG 
CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG 
GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA 
GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG 
GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT 
TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA 
AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC 
AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA 
CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT 
CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC 
CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT 
GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG 
AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA 
GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG 
AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT 
GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC 
ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG 
AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC 
GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG 
CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos2 ( XM_002935296.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935342.2 Xenopus tropicalis nitric oxide synthase 2 (nos2), mRNA.

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CloneID	OXa52170
Clone ID Related Accession (Same CDS sequence)	XM_002935296.5
Accession Version	XM_002935296.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nitric oxide synthase, inducible
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935296.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TAAAAGAGAA AGCATTTTGC 
AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC 
ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA 
TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA 
TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA 
GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG 
GTGTATATAA GAGGCCCCAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA 
ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT 
AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA 
GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA 
ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG 
TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT 
ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA 
CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC 
ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG 
ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA 
GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC 
CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG 
TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT 
TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA 
CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG 
TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT 
ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG 
CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC 
ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT 
GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA 
TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT 
GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC 
GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA 
AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG 
AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT 
TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA 
AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT 
GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA 
TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC 
TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT 
CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG 
CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG 
GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA 
GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG 
GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT 
TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA 
AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC 
AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA 
CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT 
CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC 
CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT 
GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG 
AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA 
GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG 
AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT 
GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC 
ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG 
AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC 
GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG 
CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA CAAATTAAGA 
TATCACGAAG ATATATTTGG GCCTGCTCAT AGGCCTCAGC AGTCTACAAA TGGATACCCT 
TCTCCTGTGG CTTTTAGTAC ACCAGTCTAT ACTCCACACC ATAGAATTAC AAATATGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935342.2
CDS	275..3757
Translation	MMCPWKRFMKIGTVKEKAFCNEMDINNNLHKDFEKKHSTIIKEANNFNGTKQTMAELKSVSEKRFPENITHPQKRETCPKYLKLKNYESGSILQDTLHLKAKKNLSCQSKICQGSIMNPKVYIRGPRNEPVPQDELLPQAIEFVNQYYSSFKEPQIENHLNRLETVAKEIECTGTYQLTRDELAYATKMAWRNAPRCIGRIQWSNLQVFDARDCKTAKEMFELLCRHITYSSNGGNIRSAITIFPQRTDGKHDFRVWNSQLIRYAGFQMPDGSIVGDPASIEFTELCIHLGWKPKYSRFEILPLVLQANGQDPELFEIPLDIIDEVPIEHPKYEWFKELSLKWYTLPAVANMLLEVGGLEFSACPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRLGLETHKLASLWKDQAVIELNIAVLYSFQKHNVTIMDHHSAAESFMKHLENEYRLRGGCPADWVWLVPPMSGSITPVFHQEMLNYILSPFYYYQIDAWKTHVWQDENRKPKKKEFKFSVVAKSVLFASSLLKKTVAARTKVTILYATETGKSETFAKKLQALFSYAFNAKVLCMDDYNIRNLEDESLLLVVTSTFGNGDCPGNGEKFKKAMFSCKYLKNKLRYAVFGLGSSMYPQFCAFAHTVDQRLAQLGAAQISLTGEGDELSGQEESFLNWAVQTFKAACELFKIRDRRNIHLPKCYTSTESWSAENYRLVNDNQQYDHIKALCKMHSRSIVPMKLKFRKNLQSPKSSRTTLLVKLSYENSQEVRFMPGEHAGLFAGNQPELVASVISHLKDAPPCNQHVRLETRNEQESFWTISEKIPPCSLTQALTYFLDITTPPTQLLLKKLSLLATKVTDKSRLEELSKDADEYKQWKNHNFPNFLEVLEEFPSLQVPTTFVLTQLPPLKPRYYSISSSRDMVPGEIHLTVAVVNYTTRGGQGPLHHGVCSTWLNDLDINETVPCFIRSTNTFHLPENPSVPCVLIGPGTGISPFRGFWQQRLYDLEKKGIKAGSMILLFGCQQSGMDEIYADETQYMKSKGVLKDVYTAYSRQPGQAKVYVQDILIKNLESEVCRILLEEEGHLYVCGNFEMAKNVAQTLKSMIAKRLKLNEEEAENYFSELKNKLRYHEDIFGPAHRPQQSTNGYPSPVAFSTPVYTPHHRITNMK

Target ORF information:

RefSeq Version	XM_002935296.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nitric oxide synthase 2 (nos2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935296.5

ORF Insert Sequence:

ATGATGTGCC CCTGGAAACG ATTCATGAAG ATCGGAACTG TAAAAGAGAA AGCATTTTGC 
AATGAAATGG ACATCAACAA CAATCTGCAT AAGGACTTTG AGAAAAAACA CAGCACAATC 
ATAAAAGAAG CAAACAATTT CAATGGCACG AAGCAAACAA TGGCTGAACT TAAATCTGTA 
TCAGAGAAAA GATTTCCAGA AAACATCACG CACCCGCAGA AAAGAGAGAC TTGTCCTAAA 
TATTTGAAAC TGAAGAATTA TGAAAGTGGA TCAATTCTTC AAGACACTTT ACACCTCAAA 
GCAAAGAAGA ACCTCAGCTG CCAGTCTAAA ATATGTCAGG GATCCATCAT GAACCCAAAG 
GTGTATATAA GAGGCCCCAG GAATGAGCCA GTACCACAAG ATGAACTTTT ACCTCAAGCA 
ATAGAATTTG TTAACCAGTA CTACAGCTCC TTCAAAGAGC CTCAAATAGA AAATCACCTT 
AACAGGCTGG AAACAGTGGC CAAAGAAATA GAATGTACTG GCACCTACCA GCTAACTAGA 
GATGAGCTTG CCTATGCAAC CAAGATGGCA TGGAGAAATG CACCACGCTG CATTGGACGA 
ATACAGTGGT CTAACTTACA GGTCTTTGAT GCTCGTGACT GCAAGACAGC CAAGGAAATG 
TTTGAACTCC TCTGCCGTCA TATAACTTAC TCATCAAATG GAGGAAACAT AAGATCTGCT 
ATTACCATAT TTCCTCAGAG GACTGATGGG AAACATGATT TCAGAGTGTG GAACAGTCAA 
CTTATTCGTT ATGCAGGTTT TCAAATGCCA GATGGATCAA TCGTTGGTGA CCCAGCCAGC 
ATAGAATTTA CAGAGTTGTG TATCCATCTT GGTTGGAAGC CAAAATACAG TCGGTTTGAG 
ATCCTTCCTT TGGTGCTTCA GGCAAATGGC CAAGATCCAG AGTTGTTTGA GATACCATTA 
GATATTATTG ATGAGGTGCC AATAGAACAT CCAAAGTATG AGTGGTTCAA GGAACTTAGC 
CTGAAGTGGT ATACATTGCC TGCAGTTGCC AATATGCTTC TTGAAGTTGG AGGTTTAGAG 
TTCTCTGCTT GCCCCTTCAA TGGCTGGTAC ATGGGAACAG AAATTGGTGT TCGGGATTTT 
TGTGATGTCC AGCGCTATAA TATTCTTGAG GAAGTAGGAA GGCGACTGGG CTTGGAGACA 
CACAAACTGG CCTCATTGTG GAAAGACCAA GCTGTTATTG AGCTCAACAT TGCAGTTCTG 
TATAGTTTTC AGAAACACAA CGTGACTATC ATGGACCACC ACTCTGCTGC GGAGTCCTTT 
ATGAAGCATC TGGAGAATGA GTACAGACTG CGAGGAGGTT GCCCAGCTGA TTGGGTTTGG 
CTCGTCCCCC CAATGTCAGG CAGCATCACG CCAGTGTTTC ACCAGGAAAT GCTGAATTAC 
ATTCTGAGCC CCTTTTATTA CTATCAGATC GATGCTTGGA AAACTCATGT GTGGCAGGAT 
GAAAACCGCA AACCAAAGAA GAAGGAATTT AAGTTTAGTG TTGTGGCAAA GTCTGTGCTA 
TTTGCCTCAT CACTCTTAAA GAAAACTGTG GCTGCCAGAA CCAAAGTTAC CATTCTCTAT 
GCAACAGAAA CAGGGAAATC AGAAACCTTT GCAAAGAAAC TGCAAGCACT CTTTAGTTAC 
GCCTTCAATG CCAAGGTTCT CTGTATGGAT GACTATAACA TCAGAAACTT GGAGGATGAA 
AGCCTTCTAT TGGTTGTGAC GAGCACTTTC GGAAATGGAG ACTGCCCAGG AAATGGAGAG 
AAATTCAAGA AAGCAATGTT TTCCTGCAAA TATCTAAAGA ACAAATTAAG GTATGCTGTT 
TTTGGCCTTG GATCCAGCAT GTATCCGCAG TTCTGTGCAT TTGCCCATAC AGTGGACCAA 
AGGCTGGCAC AACTCGGTGC CGCACAAATC AGCCTTACAG GTGAAGGAGA TGAGCTCAGT 
GGGCAAGAAG AGTCTTTTCT AAACTGGGCA GTTCAGACAT TTAAGGCTGC ATGTGAACTA 
TTTAAAATTC GAGACAGAAG AAATATTCAT TTACCTAAGT GTTACACCTC AACAGAAAGC 
TGGAGTGCAG AAAATTATCG TCTTGTTAAT GATAACCAAC AATATGACCA CATCAAAGCT 
CTCTGTAAAA TGCACTCCAG GAGCATAGTG CCAATGAAAC TGAAATTTAG GAAGAACTTG 
CAAAGTCCCA AATCAAGCCG TACCACACTG CTTGTAAAGC TTTCCTATGA GAATAGCCAG 
GAAGTACGGT TTATGCCAGG AGAACATGCT GGTTTGTTTG CTGGCAACCA GCCAGAGCTA 
GTAGCGAGCG TAATTAGCCA TCTGAAGGAT GCTCCGCCCT GTAATCAGCA TGTCCGACTG 
GAGACACGGA ATGAGCAGGA GAGCTTCTGG ACAATCAGTG AAAAAATCCC TCCCTGCTCT 
TTGACACAAG CACTGACATA TTTCCTAGAC ATCACTACGC CACCGACGCA GCTTCTGCTA 
AAAAAACTCT CCCTGTTGGC CACCAAGGTC ACGGATAAAA GCAGGCTGGA AGAGTTAAGC 
AAAGATGCAG ATGAATATAA ACAGTGGAAG AACCATAACT TTCCTAATTT CCTGGAGGTA 
CTGGAAGAGT TCCCATCCCT ACAAGTACCT ACAACGTTTG TGCTCACTCA GCTCCCACCT 
CTGAAGCCTA GATACTACTC TATCAGCTCC TCTCGTGATA TGGTGCCGGG GGAGATCCAC 
CTTACTGTTG CTGTTGTGAA TTACACAACC AGAGGTGGCC AGGGCCCATT ACATCATGGT 
GTCTGCAGCA CATGGCTAAA TGATCTAGAT ATCAATGAAA CAGTTCCATG CTTTATTAGG 
AGTACAAACA CATTCCATCT CCCAGAAAAC CCATCAGTTC CATGTGTACT GATTGGCCCA 
GGAACAGGAA TTTCTCCATT CAGAGGATTT TGGCAGCAGC GACTCTATGA CCTAGAAAAG 
AAAGGAATTA AGGCTGGGTC TATGATCCTA CTGTTTGGCT GCCAGCAATC AGGGATGGAT 
GAAATCTATG CGGATGAGAC ACAATACATG AAGAGTAAAG GCGTGCTGAA AGATGTGTAC 
ACTGCATATT CAAGGCAACC AGGACAAGCC AAGGTGTATG TGCAAGATAT ACTTATAAAG 
AACTTGGAGA GTGAAGTGTG TCGGATTTTG CTTGAAGAAG AAGGCCATCT GTATGTGTGC 
GGGAACTTTG AAATGGCAAA GAATGTTGCT CAGACCCTAA AAAGCATGAT TGCAAAGAGG 
CTGAAACTGA ATGAGGAAGA AGCCGAGAAT TATTTCTCTG AGTTGAAGAA CAAATTAAGA 
TATCACGAAG ATATATTTGG GCCTGCTCAT AGGCCTCAGC AGTCTACAAA TGGATACCCT 
TCTCCTGTGG CTTTTAGTAC ACCAGTCTAT ACTCCACACC ATAGAATTAC AAATATGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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nos2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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