NOS2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS2
Entrez Gene ID	282876
Full Name	nitric oxide synthase 2
Synonyms	NOS2A,iNOS
General protein information	Preferred Names nitric oxide synthase 2 Names nitric oxide synthase, inducible NOS type II NOSII inducible NO synthase inducible NOS inducible nitric oxide synthase nitric oxide synthase 2, inducible nitric oxide synthase 2A (inducible, hepatocytes) peptidyl-cysteine S-nitrosylase NOS2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024979646.1	XP_024835414.1	nitric oxide synthase, inducible isoform X2
XM_024979645.1	XP_024835413.1	nitric oxide synthase, inducible isoform X1
NM_001076799.1	NP_001070267.1	nitric oxide synthase, inducible

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NOS2	NP_001070267.1
Canis lupus familiaris (dog)	NOS2	XP_005624903.1
Xenopus tropicalis (tropical clawed frog)	nos2	XP_002935342.2
Macaca mulatta (Rhesus monkey)	NOS2	XP_001106245.1
Rattus norvegicus (Norway rat)	Nos2	NP_036743.3
Danio rerio (zebrafish)	nos2a	NP_001098407.1
Homo sapiens (human)	NOS2	NP_000616.3
Rattus norvegicus (Norway rat)	LOC497963	XP_006247011.1
Gallus gallus (chicken)	NOS2	NP_990292.1
Pan troglodytes (chimpanzee)	NOS2	XP_001148238.1
Mus musculus (house mouse)	Nos2	NP_035057.1
Danio rerio (zebrafish)	nos2b	NP_001106973.1

Gene Ontology
Bibliography

Related articles in PubMed

Relationship of bovine NOS2 gene polymorphisms to the risk of bovine tuberculosis in Holstein cattle.
Cheng Y, Huang C, Tsai HJ
The Journal of veterinary medical science78(2)281-6(2016 Feb)

Immunohistochemical expression of nitric oxide synthase enzymes (iNOS, eNOS, nNOS) in the estrual and luteal phases of the sexual cycle in the cow oviduct.
Özen A, Ergün E, Öztaş E, Ergün L, Özcan Z, Alabay B, Bayraktaroğlu AG, Kürüm A, Erdoğan E
Anatomia, histologia, embryologia42(5)384-93(2013 Oct)

Prevalence of subclinical endometritis in repeat breeding cows and mRNA expression of tumor necrosis factor alpha and inducible nitric oxide synthase in the endometrium of repeat breeding cows with and without subclinical endometritis.
Janowski T, Barański W, Lukasik K, Skarzyński D, Rudowska M, Zduńczyk S
Polish journal of veterinary sciences16(4)693-9(2013)

Placental expression and localization of endothelin-1 system and nitric oxide synthases during bovine pregnancy.
Hayashi KG, Hosoe M, Takahashi T
Animal reproduction science134(3-4)150-7(2012 Oct)

The vitamin D receptor and inducible nitric oxide synthase associated pathways in acquired resistance to Cooperia oncophora infection in cattle.
Li RW, Li C, Gasbarre LC
Veterinary research4248(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01375	NM_001076799.1 Latest version!	Bos taurus nitric oxide synthase 2 (NOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa226012	XM_024979645.1 Latest version!	Bos taurus nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa226013	XM_024979646.1 Latest version!	Bos taurus nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS2 ( NM_001076799.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001070267.1 Bos taurus nitric oxide synthase 2 (NOS2), mRNA.

Price & Availability↑

CloneID	OBa01375
Clone ID Related Accession (Same CDS sequence)	NM_001076799.1
Accession Version	NM_001076799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Bos taurus(cattle)
Product	nitric oxide synthase, inducible
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ676956.1. On or before Oct 11, 2006 this sequence version replaced XM_588018.2, XM_880927.1, XM_880948.1, XM_880967.1, XM_880990.1, XM_881006.1, XM_881023.1, XM_881043.1, XM_881060.1, XM_867963.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ676956.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTGTC CCTGGCAGTT TCTGTTCAAA ATCAAATCCC AAAAGGTGGA CTTGGCTACG 
GAACTGGACA TCAACAACAA TGTGGGAAAA TTCTACCAGC CCCCGTCCAG TCCAGTGACA 
CAGGATGACC CCAAACGTCA CAGCCCGGGC AAGCACGGGA ATGAGTCTCC CCAGCCCCTC 
ACGGGGACAG TAAAGACGTC TCCAGAATCC CTGAGCAAGC TGGACGCGCC CCCGTCAGCC 
TGTCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGAG TGACTTTCCA AGACACGCTT 
CACCAGAAGG CCAAAGGGGA TCTCTCTTGC AAGTCCAAGT CTTGCCTGGC ATCCATCATG 
AACCCCAAAA GTTTGACCAT AGGACCCCGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAGTAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC CCCCCGCTGC 
ATCGGAAGGA TCCAGTGGTC GAACCTGCAG GTCTTTGACG CCCGGAGCTG TTCCACGGCC 
CAGGAAATGT TCGAACACAT CTGCAGACAC GTGCGTTATG CCACCAACAA CGGCAACATC 
AGGTCGGCCA TCACTGTGTT CCCCCAGCGG AGCGATGGGA AGCATGACTT CCGGGTCTGG 
AACGCCCAGC TCATCCGCTA TGCCGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCCGCCAACG TGGAGTTCAC ACAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGACG TGTTGCCTCT AGTCCTGCAG GCTGACGGCC GTGACCCAGA GCTCTTCGAA 
ATCCCCCCTG ACCTTGTTCT CGAGGTGCCC ATGGAACATC CCAGGTACGA ATGGTTCCGG 
GAGCTGGAGC TCAAGTGGTA CGCCCTGCCA GCCGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTCGAGT TCCCAGGGTG CCCCTTCAAC GGGTGGTACA TGGGCACAGA AGTTGGAGTC 
CGGGACTTCT GCGACGCCCA GCGATACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC 
CTGGAAACGC ACAAGGTGGC CTCACTCTGG AAGGACCGGG CTGTCGTTGA GATCAACGTC 
GCTGTGCTCC ACAGTTTCCA GAAGCAGAAC GTGACCATCA TGGACCACCA CTCCGCTGCA 
GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGCTCCC GAGGGGGCTG CCCAGCTGAC 
TGGATTTGGC TGGTCCCCCC GATCTCTGGA AGCATCACCC CCGTGTTCCA CCAGGAGATG 
TTAAATTACG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCACGTC 
TGGCAGGACG AGAGGCGGAG ACCCCAGAGA AGAGAAATTC GATTCAAAGT CTTGGTCAAA 
GCCGTGTTCT TCGCCTCGGT GCTGATGCAT AAGGCCATGG CATCCCGGGT CAGAGCCACG 
ATCCTCTTTG CAACAGAGAC GGGGAGATCG GAAACGCTGG CCCAGGACCT GGGGGCTTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATC AGTACCAGCT GAGCCATCTG 
GAGGAGGARC AGCTGCTGCT GGTGGTGACC AGCACTTTTG GCAACGGAGA CTCCCCTGGC 
AACGGAGAGA AACTGAAGAA GTCCCTCTTG ATGCTGAAAG AACTCACCAA CACGTTCCGG 
TACGCCGTGT TTGGCCTTGG CTCCAGCATG TACCCTCAGT TCTGCGCTTT TGCTCATGAC 
ATTGACCAGA AGTTGTCCCA GCTGGGGGCT TCTCAGCTTG CCCCAACCGG GGAAGGGGAC 
GAACTCAGCG GGCAGGAGGA GGCCTTTCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGACAT TCGATGTCAG CGGCAAGCAC CACATTGAGA TCCCCAAGCT CTACACTTCC 
AATGTGACCT GGGACCCGCA GCACTACAGG CTCGTGCAGG ACTCAGAGCC TCTGGACCTC 
AACAAAGCCC TGAGCAGTAT GCACGCCAAG CATGTGTTCA CCATGAGGCT CAAATCGCAG 
CAGAATCTGC AGAGTCCGAA ATCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGTGAG 
GGCAGCCAAG CTCCAAGCTA CCTGCCGGGA GAGCACCTCG GAGTTTTCCC ATGCAACCAG 
CCGGCCCTGG TTCAAGGCAT CCTTGAGCGA GTGGTGGATG GCCCTGCCCC GCACCAGCCT 
GTGCGCCTGG AGACCCTCTG TGAGAATGGC AGCTATTGGG TCAAGGATAA GCGGCTGCCA 
CCTTGCTCAC TCAGCCAGGC TCTCACCTAT TTCCTGGACA TCACCACCCC GCCAACCCAG 
CTGCTGCTCC GAAAGCTGGC CCAGCTGGCC ACAGAAGAGG CTGAGAAGCA GAGGCTGGAG 
ACCCTATGCC AGCCCTCAGA TTACAACAAG TGGAAGTTCA CCAACAGCCC CACATTTCTG 
GAGGTGCTGG AGGAGTTCCC GTCCCTGCGG GTGTCTGCCA GCTTCCTGCT CTCCCAGCTC 
CCTATTCTGA AGCCCCGGTA CTACTCCATC AGCTCCTCCA GGGACCTCAC ACCCACGGAG 
ATCCACCTCA CTGTGGCCGT GCTCACGTAC CGCACCCGAG ATGGCCAAGG TCCCCTGCAC 
CATGGTGTCT GCAGCACGTG GCTCAGCAGC TTGAAGCCCC AAGACCCGGT GCCCTGCTTT 
GTGCGAAGTG CCAGYGGCTT CCAGCTTCCC GAGGACCGCT CCCGTCCTTG CATCCTCATT 
GGGCCCGGCA CGGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGAGGCT 
GAGCACAAAG GGCTCCAGGG CGGCCGCATG ACCCTGGTTT TCGGGTGCCG CCGCCCAGAA 
GAGGACCACC TCTACTGGGA GGAGATGCTG GAGATGGCCC GCAAGGGGGT GCTGCACGAG 
GTGCACACCG CCTATTCCCG CCTGCCTGAC CAGCCCAAGG TCTATGTTCA AGACATCCTG 
CGGCAGCGGC TGGCGGGCGA GGTGCTTCGC GTGCTCCATG AGGAGCAAGG TCACCTCTAT 
GTCTGTGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCGCA CCCTGAAGCA GCTGATGGCT 
ACTGCGCTGA GCCTGAACGA GGAACAGGTG GAGGACTATT TCTTCCAGCT CAAGAACCAG 
AAGCGCTATC ATGAAGACAT CTTTGGTGCT GTATTTCCCT ATGAGGTGAA AAAGGATGGG 
GCAGCAGGGC TGCCCAGCAA TCCCAGAGCT CCAGGCGCCC ACAGAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070267.1
CDS	1..3471
Translation	MACPWQFLFKIKSQKVDLATELDINNNVGKFYQPPSSPVTQDDPKRHSPGKHGNESPQPLTGTVKTSPESLSKLDAPPSACPRHVRIKNWGSGVTFQDTLHQKAKGDLSCKSKSCLASIMNPKSLTIGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAQEMFEHICRHVRYATNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVLPLVLQADGRDPELFEIPPDLVLEVPMEHPRYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEVGVRDFCDAQRYNILEEVGRRMGLETHKVASLWKDRAVVEINVAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPISGSITPVFHQEMLNYVLSPFYYYQVEPWKTHVWQDERRRPQRREIRFKVLVKAVFFASVLMHKAMASRVRATILFATETGRSETLAQDLGALFSCAFNPKVLCMDQYQLSHLEEnullQLLLVVTSTFGNGDSPGNGEKLKKSLLMLKELTNTFRYAVFGLGSSMYPQFCAFAHDIDQKLSQLGASQLAPTGEGDELSGQEEAFRSWAVQTFKAACETFDVSGKHHIEIPKLYTSNVTWDPQHYRLVQDSEPLDLNKALSSMHAKHVFTMRLKSQQNLQSPKSSRTTLLVELSCEGSQAPSYLPGEHLGVFPCNQPALVQGILERVVDGPAPHQPVRLETLCENGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLLLRKLAQLATEEAEKQRLETLCQPSDYNKWKFTNSPTFLEVLEEFPSLRVSASFLLSQLPILKPRYYSISSSRDLTPTEIHLTVAVLTYRTRDGQGPLHHGVCSTWLSSLKPQDPVPCFVRSAnullGFQLPEDRSRPCILIGPGTGIAPFRSFWQQRLHEAEHKGLQGGRMTLVFGCRRPEEDHLYWEEMLEMARKGVLHEVHTAYSRLPDQPKVYVQDILRQRLAGEVLRVLHEEQGHLYVCGDVRMARDVARTLKQLMATALSLNEEQVEDYFFQLKNQKRYHEDIFGAVFPYEVKKDGAAGLPSNPRAPGAHRS

Target ORF information:

RefSeq Version	NM_001076799.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nitric oxide synthase 2 (NOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076799.1

ORF Insert Sequence:

ATGGCCTGTC CCTGGCAGTT TCTGTTCAAA ATCAAATCCC AAAAGGTGGA CTTGGCTACG 
GAACTGGACA TCAACAACAA TGTGGGAAAA TTCTACCAGC CCCCGTCCAG TCCAGTGACA 
CAGGATGACC CCAAACGTCA CAGCCCGGGC AAGCACGGGA ATGAGTCTCC CCAGCCCCTC 
ACGGGGACAG TAAAGACGTC TCCAGAATCC CTGAGCAAGC TGGACGCGCC CCCGTCAGCC 
TGTCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGAG TGACTTTCCA AGACACGCTT 
CACCAGAAGG CCAAAGGGGA TCTCTCTTGC AAGTCCAAGT CTTGCCTGGC ATCCATCATG 
AACCCCAAAA GTTTGACCAT AGGACCCCGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAGTAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC CCCCCGCTGC 
ATCGGAAGGA TCCAGTGGTC GAACCTGCAG GTCTTTGACG CCCGGAGCTG TTCCACGGCC 
CAGGAAATGT TCGAACACAT CTGCAGACAC GTGCGTTATG CCACCAACAA CGGCAACATC 
AGGTCGGCCA TCACTGTGTT CCCCCAGCGG AGCGATGGGA AGCATGACTT CCGGGTCTGG 
AACGCCCAGC TCATCCGCTA TGCCGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCCGCCAACG TGGAGTTCAC ACAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGACG TGTTGCCTCT AGTCCTGCAG GCTGACGGCC GTGACCCAGA GCTCTTCGAA 
ATCCCCCCTG ACCTTGTTCT CGAGGTGCCC ATGGAACATC CCAGGTACGA ATGGTTCCGG 
GAGCTGGAGC TCAAGTGGTA CGCCCTGCCA GCCGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTCGAGT TCCCAGGGTG CCCCTTCAAC GGGTGGTACA TGGGCACAGA AGTTGGAGTC 
CGGGACTTCT GCGACGCCCA GCGATACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC 
CTGGAAACGC ACAAGGTGGC CTCACTCTGG AAGGACCGGG CTGTCGTTGA GATCAACGTC 
GCTGTGCTCC ACAGTTTCCA GAAGCAGAAC GTGACCATCA TGGACCACCA CTCCGCTGCA 
GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGCTCCC GAGGGGGCTG CCCAGCTGAC 
TGGATTTGGC TGGTCCCCCC GATCTCTGGA AGCATCACCC CCGTGTTCCA CCAGGAGATG 
TTAAATTACG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCACGTC 
TGGCAGGACG AGAGGCGGAG ACCCCAGAGA AGAGAAATTC GATTCAAAGT CTTGGTCAAA 
GCCGTGTTCT TCGCCTCGGT GCTGATGCAT AAGGCCATGG CATCCCGGGT CAGAGCCACG 
ATCCTCTTTG CAACAGAGAC GGGGAGATCG GAAACGCTGG CCCAGGACCT GGGGGCTTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATC AGTACCAGCT GAGCCATCTG 
GAGGAGGARC AGCTGCTGCT GGTGGTGACC AGCACTTTTG GCAACGGAGA CTCCCCTGGC 
AACGGAGAGA AACTGAAGAA GTCCCTCTTG ATGCTGAAAG AACTCACCAA CACGTTCCGG 
TACGCCGTGT TTGGCCTTGG CTCCAGCATG TACCCTCAGT TCTGCGCTTT TGCTCATGAC 
ATTGACCAGA AGTTGTCCCA GCTGGGGGCT TCTCAGCTTG CCCCAACCGG GGAAGGGGAC 
GAACTCAGCG GGCAGGAGGA GGCCTTTCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGACAT TCGATGTCAG CGGCAAGCAC CACATTGAGA TCCCCAAGCT CTACACTTCC 
AATGTGACCT GGGACCCGCA GCACTACAGG CTCGTGCAGG ACTCAGAGCC TCTGGACCTC 
AACAAAGCCC TGAGCAGTAT GCACGCCAAG CATGTGTTCA CCATGAGGCT CAAATCGCAG 
CAGAATCTGC AGAGTCCGAA ATCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGTGAG 
GGCAGCCAAG CTCCAAGCTA CCTGCCGGGA GAGCACCTCG GAGTTTTCCC ATGCAACCAG 
CCGGCCCTGG TTCAAGGCAT CCTTGAGCGA GTGGTGGATG GCCCTGCCCC GCACCAGCCT 
GTGCGCCTGG AGACCCTCTG TGAGAATGGC AGCTATTGGG TCAAGGATAA GCGGCTGCCA 
CCTTGCTCAC TCAGCCAGGC TCTCACCTAT TTCCTGGACA TCACCACCCC GCCAACCCAG 
CTGCTGCTCC GAAAGCTGGC CCAGCTGGCC ACAGAAGAGG CTGAGAAGCA GAGGCTGGAG 
ACCCTATGCC AGCCCTCAGA TTACAACAAG TGGAAGTTCA CCAACAGCCC CACATTTCTG 
GAGGTGCTGG AGGAGTTCCC GTCCCTGCGG GTGTCTGCCA GCTTCCTGCT CTCCCAGCTC 
CCTATTCTGA AGCCCCGGTA CTACTCCATC AGCTCCTCCA GGGACCTCAC ACCCACGGAG 
ATCCACCTCA CTGTGGCCGT GCTCACGTAC CGCACCCGAG ATGGCCAAGG TCCCCTGCAC 
CATGGTGTCT GCAGCACGTG GCTCAGCAGC TTGAAGCCCC AAGACCCGGT GCCCTGCTTT 
GTGCGAAGTG CCAGYGGCTT CCAGCTTCCC GAGGACCGCT CCCGTCCTTG CATCCTCATT 
GGGCCCGGCA CGGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGAGGCT 
GAGCACAAAG GGCTCCAGGG CGGCCGCATG ACCCTGGTTT TCGGGTGCCG CCGCCCAGAA 
GAGGACCACC TCTACTGGGA GGAGATGCTG GAGATGGCCC GCAAGGGGGT GCTGCACGAG 
GTGCACACCG CCTATTCCCG CCTGCCTGAC CAGCCCAAGG TCTATGTTCA AGACATCCTG 
CGGCAGCGGC TGGCGGGCGA GGTGCTTCGC GTGCTCCATG AGGAGCAAGG TCACCTCTAT 
GTCTGTGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCGCA CCCTGAAGCA GCTGATGGCT 
ACTGCGCTGA GCCTGAACGA GGAACAGGTG GAGGACTATT TCTTCCAGCT CAAGAACCAG 
AAGCGCTATC ATGAAGACAT CTTTGGTGCT GTATTTCCCT ATGAGGTGAA AAAGGATGGG 
GCAGCAGGGC TGCCCAGCAA TCCCAGAGCT CCAGGCGCCC ACAGAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_024979645.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835413.1 Bos taurus nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226012
Clone ID Related Accession (Same CDS sequence)	XM_024979645.1
Accession Version	XM_024979645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	nitric oxide synthase, inducible isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGTC CCTGGCAGTT TCTGTTCAAA ATCAAATCCC AAAAGGTGGA CTTGGCTACG 
GAACTGGACA TCAACAACAA TGTGGGAAAA TTCTACCAGC CCCCGTCCAG TCCAGTGACA 
CAGGATGACC CCAAACGTCA CAGCCCGGGC AAGCACGGGA ATGAGTCTCC CCAGCCCCTC 
ACGGGGACAG TAAAGACGTC TCCAGAATCC CTGAGCAAGC TGGACGCGCC CCCGTCAGCC 
TGTCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGAG TGACTTTCCA AGACACGCTT 
CACCAGAAGG CCAAAGGGGA TCTCTCTTGC AAGTCCAAGT CTTGCCTGGC ATCCATCATG 
AACCCCAAAA GTTTGACCAT AGGACCCCGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAGTAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC CCCCCGCTGC 
ATCGGAAGGA TCCAGTGGTC GAACCTGCAG GTCTTTGACG CCCGGAGCTG TTCCACGGCC 
CAGGAAATGT TCGAACACAT CTGCAGACAC GTGCGTTATG CCACCAACAA CGGCAACATC 
AGGTCGGCCA TCACTGTGTT CCCCCAGCGG AGCGATGGGA AGCATGACTT CCGGGTCTGG 
AACGCCCAGC TCATCCGCTA TGCCGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCCGCCAACG TGGAGTTCAC ACAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGACG TGTTGCCTCT AGTCCTGCAG GCTGACGGCC GTGACCCAGA GCTCTTCGAA 
ATCCCCCCTG ACCTTGTTCT CGAGGTGCCC ATGGAACATC CCAGGTACGA ATGGTTCCGG 
GAGCTGGAGC TCAAGTGGTA CGCCCTGCCA GCCGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTCGAGT TCCCAGGGTG CCCCTTCAAC GGGTGGTACA TGGGCACAGA AGTTGGAGTC 
CGGGACTTCT GCGACGCCCA GCGATACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC 
CTGGAAACGC ACAAGGTGGC CTCACTCTGG AAGGACCGGG CTGTCGTTGA GATCAACGTC 
GCTGTGCTCC ACAGTTTCCA GAAGCAGAAC GTGACCATCA TGGACCACCA CTCCGCTGCA 
GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGCTCCC GAGGGGGCTG CCCAGCTGAC 
TGGATTTGGC TGGTCCCCCC GATCTCTGGA AGCATCACCC CCGTGTTCCA CCAGGAGATG 
TTAAATTACG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCACGTC 
TGGCAGGACG AGAGGCGGAG ACCCCAGAGA AGAGAGATTC GATTCAAAGT CTTGGTCAAA 
GCCGTGTTCT TCGCCTCGGT GCTGATGCAT AAGACCATGG CATCCCGGGT CAGAGCCACG 
ATCCTCTTTG CAACAGAGAC GGGGAGATCG GAAACGCTGG CCCAGGACCT GGGGGCTTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATC AGTACCAGCT GAGCCATCTG 
GAGGAGGAGC AGCTGCTGCT GGTGGTGACC AGCACTTTTG GCAACGGAGA CTCCCCTGGC 
AACGGAGAGA AACTGAAGAA GTCCCTCTTG ATGCTGAAAG AACTCACCAA CACGTTCCGG 
TACGCCGTGT TTGGCCTTGG CTCCAGCATG TACCCTCAGT TCTGCGCTTT TGCTCATGAC 
ATTGACCAGA AGTTGTCCCA GCTGGGGGCT TCTCAGCTTG TCCCAACCGG GGAAGGGGAC 
GAACTCAGCG GGCAGGAGGA GGCCTTTCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGACAT TCGATGTCAG CGGCAAGCAC CACATTGAGA TCCCCAAGCT CTACACTTCC 
AATGTGACCT GGGACCCGCA GCACTACAGG CTCGTGCAGG ACTCAGAGCC TCTGGACCTC 
AACAAAGCCC TGAGCAGTAT GCACGCCAAG CATGTGTTCA CCATGAGGCT CAAATCGCAG 
CAGAATCTGC AGAGTCCGAA ATCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGTGAG 
GGCAGCCAAG CTCCAAGCTA CCTGCCGGGA GAGCACCTCG GAGTTTTCCC ATGCAACCAG 
CCGGCCCTGG TTCAAGGCAT CCTTGAGCGA GTGGTGGATG GCCCTGCCCC GCACCAGCCT 
GTGCGCCTGG AGACCCTCTG TGAGAATGGC AGCTATTGGG TCAAGGATAA GCGGCTGCCG 
CCTTGCTCAC TCAGCCAGGC TCTCACCTAT TTCCTGGACA TCACCACCCC GCCAACCCAG 
CTGCTGCTCC GAAAGCTGGC CCAGCTGGCC ACAGAAGAGG CTGAGAAGCA GAGGCTGGAG 
ACCCTATGCC AGCCCTCAGA TTACAACAAG TGGAAGTTCA CCAACAGCCC CACATTTCTG 
GAGGTGCTGG AGGAGTTCCC GTCCCTGCGG GTGTCTGCCA GCTTCCTGCT CTCCCAGCTC 
CCTATTCTGA AGCCCCGGTA CTACTCCATC AGCTCCTCCA GGGACCTCAC ACCCACGGAG 
ATCCACCTCA CTGTGGCCGT GCTCACGTAC CGCACCCGAG ATGGCCAAGG TCCCCTGCAC 
CATGGTGTCT GCAGCACGTG GCTCAGCAGC TTGAAGCCCC AAGACCCGGT GCCCTGCTTT 
GTGCGAAGTG CCAGCGGCTT CCAGCTTCCC GAGGACCGCT CCCGTCCTTG CATCCTCATT 
GGGCCCGGCA CGGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGAGGCT 
GAGCACAAAG GGCTCCAGGG CGGCCGCATG ACCCTGGTTT TCGGGTGCCG CCGCCCAGAA 
GAGGACCACC TCTACTGGGA GGAGATGCTG GAGATGGCCC GCAAGGGGGT GCTGCACGAG 
GTGCACACCG CCTATTCCCG CCTGCCTGAC CAGCCCAAGG TCTATGTTCA AGACATCCTG 
CGGCAGCGGC TGGCGGGCGA GGTGCTTCGC GTGCTCCATG AGGAGCAAGG TCACCTCTAT 
GTCTGTGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCGCA CCCTGAAGCA GCTGATGGCT 
ACTGCGCTGA GCCTGAACGA GGAACAGGTG GAGGACTATT TCTTCCAGCT CAAGAACCAG 
AAGCGCTATC ATGAAGACAT CTTTGGTGCT GTATTTCCCT ATGAGGTGAA AAAGGATGGG 
GCAGCAGGGC TGCCCAGCAA TCCCAGAGCT CCAGGCGCCC ACAGAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835413.1
CDS	326..3796
Translation	MACPWQFLFKIKSQKVDLATELDINNNVGKFYQPPSSPVTQDDPKRHSPGKHGNESPQPLTGTVKTSPESLSKLDAPPSACPRHVRIKNWGSGVTFQDTLHQKAKGDLSCKSKSCLASIMNPKSLTIGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAQEMFEHICRHVRYATNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVLPLVLQADGRDPELFEIPPDLVLEVPMEHPRYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEVGVRDFCDAQRYNILEEVGRRMGLETHKVASLWKDRAVVEINVAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPISGSITPVFHQEMLNYVLSPFYYYQVEPWKTHVWQDERRRPQRREIRFKVLVKAVFFASVLMHKTMASRVRATILFATETGRSETLAQDLGALFSCAFNPKVLCMDQYQLSHLEEEQLLLVVTSTFGNGDSPGNGEKLKKSLLMLKELTNTFRYAVFGLGSSMYPQFCAFAHDIDQKLSQLGASQLVPTGEGDELSGQEEAFRSWAVQTFKAACETFDVSGKHHIEIPKLYTSNVTWDPQHYRLVQDSEPLDLNKALSSMHAKHVFTMRLKSQQNLQSPKSSRTTLLVELSCEGSQAPSYLPGEHLGVFPCNQPALVQGILERVVDGPAPHQPVRLETLCENGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLLLRKLAQLATEEAEKQRLETLCQPSDYNKWKFTNSPTFLEVLEEFPSLRVSASFLLSQLPILKPRYYSISSSRDLTPTEIHLTVAVLTYRTRDGQGPLHHGVCSTWLSSLKPQDPVPCFVRSASGFQLPEDRSRPCILIGPGTGIAPFRSFWQQRLHEAEHKGLQGGRMTLVFGCRRPEEDHLYWEEMLEMARKGVLHEVHTAYSRLPDQPKVYVQDILRQRLAGEVLRVLHEEQGHLYVCGDVRMARDVARTLKQLMATALSLNEEQVEDYFFQLKNQKRYHEDIFGAVFPYEVKKDGAAGLPSNPRAPGAHRS

Target ORF information:

RefSeq Version	XM_024979645.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979645.1

ORF Insert Sequence:

ATGGCCTGTC CCTGGCAGTT TCTGTTCAAA ATCAAATCCC AAAAGGTGGA CTTGGCTACG 
GAACTGGACA TCAACAACAA TGTGGGAAAA TTCTACCAGC CCCCGTCCAG TCCAGTGACA 
CAGGATGACC CCAAACGTCA CAGCCCGGGC AAGCACGGGA ATGAGTCTCC CCAGCCCCTC 
ACGGGGACAG TAAAGACGTC TCCAGAATCC CTGAGCAAGC TGGACGCGCC CCCGTCAGCC 
TGTCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGAG TGACTTTCCA AGACACGCTT 
CACCAGAAGG CCAAAGGGGA TCTCTCTTGC AAGTCCAAGT CTTGCCTGGC ATCCATCATG 
AACCCCAAAA GTTTGACCAT AGGACCCCGG GACAAGCCAA CCCCCCCAGA CGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAGTAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCAGTAACA AAGGAGATAG AAACAACAGG AACCTACCAG 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAACGC CCCCCGCTGC 
ATCGGAAGGA TCCAGTGGTC GAACCTGCAG GTCTTTGACG CCCGGAGCTG TTCCACGGCC 
CAGGAAATGT TCGAACACAT CTGCAGACAC GTGCGTTATG CCACCAACAA CGGCAACATC 
AGGTCGGCCA TCACTGTGTT CCCCCAGCGG AGCGATGGGA AGCATGACTT CCGGGTCTGG 
AACGCCCAGC TCATCCGCTA TGCCGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCCGCCAACG TGGAGTTCAC ACAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGACG TGTTGCCTCT AGTCCTGCAG GCTGACGGCC GTGACCCAGA GCTCTTCGAA 
ATCCCCCCTG ACCTTGTTCT CGAGGTGCCC ATGGAACATC CCAGGTACGA ATGGTTCCGG 
GAGCTGGAGC TCAAGTGGTA CGCCCTGCCA GCCGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTCGAGT TCCCAGGGTG CCCCTTCAAC GGGTGGTACA TGGGCACAGA AGTTGGAGTC 
CGGGACTTCT GCGACGCCCA GCGATACAAC ATCCTGGAGG AAGTGGGCAG AAGGATGGGC 
CTGGAAACGC ACAAGGTGGC CTCACTCTGG AAGGACCGGG CTGTCGTTGA GATCAACGTC 
GCTGTGCTCC ACAGTTTCCA GAAGCAGAAC GTGACCATCA TGGACCACCA CTCCGCTGCA 
GAGTCCTTCA TGAAGTACAT GCAGAATGAG TACCGCTCCC GAGGGGGCTG CCCAGCTGAC 
TGGATTTGGC TGGTCCCCCC GATCTCTGGA AGCATCACCC CCGTGTTCCA CCAGGAGATG 
TTAAATTACG TCCTGTCCCC TTTCTACTAC TACCAGGTGG AGCCCTGGAA AACCCACGTC 
TGGCAGGACG AGAGGCGGAG ACCCCAGAGA AGAGAGATTC GATTCAAAGT CTTGGTCAAA 
GCCGTGTTCT TCGCCTCGGT GCTGATGCAT AAGACCATGG CATCCCGGGT CAGAGCCACG 
ATCCTCTTTG CAACAGAGAC GGGGAGATCG GAAACGCTGG CCCAGGACCT GGGGGCTTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTCTC TGCATGGATC AGTACCAGCT GAGCCATCTG 
GAGGAGGAGC AGCTGCTGCT GGTGGTGACC AGCACTTTTG GCAACGGAGA CTCCCCTGGC 
AACGGAGAGA AACTGAAGAA GTCCCTCTTG ATGCTGAAAG AACTCACCAA CACGTTCCGG 
TACGCCGTGT TTGGCCTTGG CTCCAGCATG TACCCTCAGT TCTGCGCTTT TGCTCATGAC 
ATTGACCAGA AGTTGTCCCA GCTGGGGGCT TCTCAGCTTG TCCCAACCGG GGAAGGGGAC 
GAACTCAGCG GGCAGGAGGA GGCCTTTCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGACAT TCGATGTCAG CGGCAAGCAC CACATTGAGA TCCCCAAGCT CTACACTTCC 
AATGTGACCT GGGACCCGCA GCACTACAGG CTCGTGCAGG ACTCAGAGCC TCTGGACCTC 
AACAAAGCCC TGAGCAGTAT GCACGCCAAG CATGTGTTCA CCATGAGGCT CAAATCGCAG 
CAGAATCTGC AGAGTCCGAA ATCCAGCCGC ACCACCCTCC TGGTGGAACT CTCCTGTGAG 
GGCAGCCAAG CTCCAAGCTA CCTGCCGGGA GAGCACCTCG GAGTTTTCCC ATGCAACCAG 
CCGGCCCTGG TTCAAGGCAT CCTTGAGCGA GTGGTGGATG GCCCTGCCCC GCACCAGCCT 
GTGCGCCTGG AGACCCTCTG TGAGAATGGC AGCTATTGGG TCAAGGATAA GCGGCTGCCG 
CCTTGCTCAC TCAGCCAGGC TCTCACCTAT TTCCTGGACA TCACCACCCC GCCAACCCAG 
CTGCTGCTCC GAAAGCTGGC CCAGCTGGCC ACAGAAGAGG CTGAGAAGCA GAGGCTGGAG 
ACCCTATGCC AGCCCTCAGA TTACAACAAG TGGAAGTTCA CCAACAGCCC CACATTTCTG 
GAGGTGCTGG AGGAGTTCCC GTCCCTGCGG GTGTCTGCCA GCTTCCTGCT CTCCCAGCTC 
CCTATTCTGA AGCCCCGGTA CTACTCCATC AGCTCCTCCA GGGACCTCAC ACCCACGGAG 
ATCCACCTCA CTGTGGCCGT GCTCACGTAC CGCACCCGAG ATGGCCAAGG TCCCCTGCAC 
CATGGTGTCT GCAGCACGTG GCTCAGCAGC TTGAAGCCCC AAGACCCGGT GCCCTGCTTT 
GTGCGAAGTG CCAGCGGCTT CCAGCTTCCC GAGGACCGCT CCCGTCCTTG CATCCTCATT 
GGGCCCGGCA CGGGCATCGC CCCCTTCCGC AGTTTCTGGC AGCAGCGGCT CCATGAGGCT 
GAGCACAAAG GGCTCCAGGG CGGCCGCATG ACCCTGGTTT TCGGGTGCCG CCGCCCAGAA 
GAGGACCACC TCTACTGGGA GGAGATGCTG GAGATGGCCC GCAAGGGGGT GCTGCACGAG 
GTGCACACCG CCTATTCCCG CCTGCCTGAC CAGCCCAAGG TCTATGTTCA AGACATCCTG 
CGGCAGCGGC TGGCGGGCGA GGTGCTTCGC GTGCTCCATG AGGAGCAAGG TCACCTCTAT 
GTCTGTGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCGCA CCCTGAAGCA GCTGATGGCT 
ACTGCGCTGA GCCTGAACGA GGAACAGGTG GAGGACTATT TCTTCCAGCT CAAGAACCAG 
AAGCGCTATC ATGAAGACAT CTTTGGTGCT GTATTTCCCT ATGAGGTGAA AAAGGATGGG 
GCAGCAGGGC TGCCCAGCAA TCCCAGAGCT CCAGGCGCCC ACAGAAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_024979646.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835414.1 Bos taurus nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

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CloneID	OBa226013
Clone ID Related Accession (Same CDS sequence)	XM_024979646.1
Accession Version	XM_024979646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	nitric oxide synthase, inducible isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGAGG GGCTGGGCGC CGCGTGCGAG GCCGCCCGGC CCCGGCTCTG CCGGCTCCTC 
CGCAGGATCC TCCGGCCGCA GCCCGCCTGC CGCCTCCGCC GAGCCAGTCC AGTGACACAG 
GATGACCCCA AACGTCACAG CCCGGGCAAG CACGGGAATG AGTCTCCCCA GCCCCTCACG 
GGGACAGTAA AGACGTCTCC AGAATCCCTG AGCAAGCTGG ACGCGCCCCC GTCAGCCTGT 
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGAGTGA CTTTCCAAGA CACGCTTCAC 
CAGAAGGCCA AAGGGGATCT CTCTTGCAAG TCCAAGTCTT GCCTGGCATC CATCATGAAC 
CCCAAAAGTT TGACCATAGG ACCCCGGGAC AAGCCAACCC CCCCAGACGA GCTTCTACCT 
CAAGCTATCG AATTTGTCAA CCAGTATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA 
CATCTGGCCA GGGTGGAAGC AGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAGCTG 
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAACGCCCC CCGCTGCATC 
GGAAGGATCC AGTGGTCGAA CCTGCAGGTC TTTGACGCCC GGAGCTGTTC CACGGCCCAG 
GAAATGTTCG AACACATCTG CAGACACGTG CGTTATGCCA CCAACAACGG CAACATCAGG 
TCGGCCATCA CTGTGTTCCC CCAGCGGAGC GATGGGAAGC ATGACTTCCG GGTCTGGAAC 
GCCCAGCTCA TCCGCTATGC CGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCC 
GCCAACGTGG AGTTCACACA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC 
TTCGACGTGT TGCCTCTAGT CCTGCAGGCT GACGGCCGTG ACCCAGAGCT CTTCGAAATC 
CCCCCTGACC TTGTTCTCGA GGTGCCCATG GAACATCCCA GGTACGAATG GTTCCGGGAG 
CTGGAGCTCA AGTGGTACGC CCTGCCAGCC GTGGCCAACA TGCTGCTTGA GGTGGGCGGC 
CTCGAGTTCC CAGGGTGCCC CTTCAACGGG TGGTACATGG GCACAGAAGT TGGAGTCCGG 
GACTTCTGCG ACGCCCAGCG ATACAACATC CTGGAGGAAG TGGGCAGAAG GATGGGCCTG 
GAAACGCACA AGGTGGCCTC ACTCTGGAAG GACCGGGCTG TCGTTGAGAT CAACGTCGCT 
GTGCTCCACA GTTTCCAGAA GCAGAACGTG ACCATCATGG ACCACCACTC CGCTGCAGAG 
TCCTTCATGA AGTACATGCA GAATGAGTAC CGCTCCCGAG GGGGCTGCCC AGCTGACTGG 
ATTTGGCTGG TCCCCCCGAT CTCTGGAAGC ATCACCCCCG TGTTCCACCA GGAGATGTTA 
AATTACGTCC TGTCCCCTTT CTACTACTAC CAGGTGGAGC CCTGGAAAAC CCACGTCTGG 
CAGGACGAGA GGCGGAGACC CCAGAGAAGA GAGATTCGAT TCAAAGTCTT GGTCAAAGCC 
GTGTTCTTCG CCTCGGTGCT GATGCATAAG ACCATGGCAT CCCGGGTCAG AGCCACGATC 
CTCTTTGCAA CAGAGACGGG GAGATCGGAA ACGCTGGCCC AGGACCTGGG GGCTTTGTTC 
AGCTGTGCCT TCAACCCCAA GGTTCTCTGC ATGGATCAGT ACCAGCTGAG CCATCTGGAG 
GAGGAGCAGC TGCTGCTGGT GGTGACCAGC ACTTTTGGCA ACGGAGACTC CCCTGGCAAC 
GGAGAGAAAC TGAAGAAGTC CCTCTTGATG CTGAAAGAAC TCACCAACAC GTTCCGGTAC 
GCCGTGTTTG GCCTTGGCTC CAGCATGTAC CCTCAGTTCT GCGCTTTTGC TCATGACATT 
GACCAGAAGT TGTCCCAGCT GGGGGCTTCT CAGCTTGTCC CAACCGGGGA AGGGGACGAA 
CTCAGCGGGC AGGAGGAGGC CTTTCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT 
GAGACATTCG ATGTCAGCGG CAAGCACCAC ATTGAGATCC CCAAGCTCTA CACTTCCAAT 
GTGACCTGGG ACCCGCAGCA CTACAGGCTC GTGCAGGACT CAGAGCCTCT GGACCTCAAC 
AAAGCCCTGA GCAGTATGCA CGCCAAGCAT GTGTTCACCA TGAGGCTCAA ATCGCAGCAG 
AATCTGCAGA GTCCGAAATC CAGCCGCACC ACCCTCCTGG TGGAACTCTC CTGTGAGGGC 
AGCCAAGCTC CAAGCTACCT GCCGGGAGAG CACCTCGGAG TTTTCCCATG CAACCAGCCG 
GCCCTGGTTC AAGGCATCCT TGAGCGAGTG GTGGATGGCC CTGCCCCGCA CCAGCCTGTG 
CGCCTGGAGA CCCTCTGTGA GAATGGCAGC TATTGGGTCA AGGATAAGCG GCTGCCGCCT 
TGCTCACTCA GCCAGGCTCT CACCTATTTC CTGGACATCA CCACCCCGCC AACCCAGCTG 
CTGCTCCGAA AGCTGGCCCA GCTGGCCACA GAAGAGGCTG AGAAGCAGAG GCTGGAGACC 
CTATGCCAGC CCTCAGATTA CAACAAGTGG AAGTTCACCA ACAGCCCCAC ATTTCTGGAG 
GTGCTGGAGG AGTTCCCGTC CCTGCGGGTG TCTGCCAGCT TCCTGCTCTC CCAGCTCCCT 
ATTCTGAAGC CCCGGTACTA CTCCATCAGC TCCTCCAGGG ACCTCACACC CACGGAGATC 
CACCTCACTG TGGCCGTGCT CACGTACCGC ACCCGAGATG GCCAAGGTCC CCTGCACCAT 
GGTGTCTGCA GCACGTGGCT CAGCAGCTTG AAGCCCCAAG ACCCGGTGCC CTGCTTTGTG 
CGAAGTGCCA GCGGCTTCCA GCTTCCCGAG GACCGCTCCC GTCCTTGCAT CCTCATTGGG 
CCCGGCACGG GCATCGCCCC CTTCCGCAGT TTCTGGCAGC AGCGGCTCCA TGAGGCTGAG 
CACAAAGGGC TCCAGGGCGG CCGCATGACC CTGGTTTTCG GGTGCCGCCG CCCAGAAGAG 
GACCACCTCT ACTGGGAGGA GATGCTGGAG ATGGCCCGCA AGGGGGTGCT GCACGAGGTG 
CACACCGCCT ATTCCCGCCT GCCTGACCAG CCCAAGGTCT ATGTTCAAGA CATCCTGCGG 
CAGCGGCTGG CGGGCGAGGT GCTTCGCGTG CTCCATGAGG AGCAAGGTCA CCTCTATGTC 
TGTGGGGATG TGCGCATGGC CCGGGACGTG GCCCGCACCC TGAAGCAGCT GATGGCTACT 
GCGCTGAGCC TGAACGAGGA ACAGGTGGAG GACTATTTCT TCCAGCTCAA GAACCAGAAG 
CGCTATCATG AAGACATCTT TGGTGCTGTA TTTCCCTATG AGGTGAAAAA GGATGGGGCA 
GCAGGGCTGC CCAGCAATCC CAGAGCTCCA GGCGCCCACA GAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835414.1
CDS	109..3576
Translation	MREGLGAACEAARPRLCRLLRRILRPQPACRLRRASPVTQDDPKRHSPGKHGNESPQPLTGTVKTSPESLSKLDAPPSACPRHVRIKNWGSGVTFQDTLHQKAKGDLSCKSKSCLASIMNPKSLTIGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAQEMFEHICRHVRYATNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVLPLVLQADGRDPELFEIPPDLVLEVPMEHPRYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEVGVRDFCDAQRYNILEEVGRRMGLETHKVASLWKDRAVVEINVAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPISGSITPVFHQEMLNYVLSPFYYYQVEPWKTHVWQDERRRPQRREIRFKVLVKAVFFASVLMHKTMASRVRATILFATETGRSETLAQDLGALFSCAFNPKVLCMDQYQLSHLEEEQLLLVVTSTFGNGDSPGNGEKLKKSLLMLKELTNTFRYAVFGLGSSMYPQFCAFAHDIDQKLSQLGASQLVPTGEGDELSGQEEAFRSWAVQTFKAACETFDVSGKHHIEIPKLYTSNVTWDPQHYRLVQDSEPLDLNKALSSMHAKHVFTMRLKSQQNLQSPKSSRTTLLVELSCEGSQAPSYLPGEHLGVFPCNQPALVQGILERVVDGPAPHQPVRLETLCENGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLLLRKLAQLATEEAEKQRLETLCQPSDYNKWKFTNSPTFLEVLEEFPSLRVSASFLLSQLPILKPRYYSISSSRDLTPTEIHLTVAVLTYRTRDGQGPLHHGVCSTWLSSLKPQDPVPCFVRSASGFQLPEDRSRPCILIGPGTGIAPFRSFWQQRLHEAEHKGLQGGRMTLVFGCRRPEEDHLYWEEMLEMARKGVLHEVHTAYSRLPDQPKVYVQDILRQRLAGEVLRVLHEEQGHLYVCGDVRMARDVARTLKQLMATALSLNEEQVEDYFFQLKNQKRYHEDIFGAVFPYEVKKDGAAGLPSNPRAPGAHRS

Target ORF information:

RefSeq Version	XM_024979646.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979646.1

ORF Insert Sequence:

ATGCGCGAGG GGCTGGGCGC CGCGTGCGAG GCCGCCCGGC CCCGGCTCTG CCGGCTCCTC 
CGCAGGATCC TCCGGCCGCA GCCCGCCTGC CGCCTCCGCC GAGCCAGTCC AGTGACACAG 
GATGACCCCA AACGTCACAG CCCGGGCAAG CACGGGAATG AGTCTCCCCA GCCCCTCACG 
GGGACAGTAA AGACGTCTCC AGAATCCCTG AGCAAGCTGG ACGCGCCCCC GTCAGCCTGT 
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGAGTGA CTTTCCAAGA CACGCTTCAC 
CAGAAGGCCA AAGGGGATCT CTCTTGCAAG TCCAAGTCTT GCCTGGCATC CATCATGAAC 
CCCAAAAGTT TGACCATAGG ACCCCGGGAC AAGCCAACCC CCCCAGACGA GCTTCTACCT 
CAAGCTATCG AATTTGTCAA CCAGTATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA 
CATCTGGCCA GGGTGGAAGC AGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAGCTG 
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAACGCCCC CCGCTGCATC 
GGAAGGATCC AGTGGTCGAA CCTGCAGGTC TTTGACGCCC GGAGCTGTTC CACGGCCCAG 
GAAATGTTCG AACACATCTG CAGACACGTG CGTTATGCCA CCAACAACGG CAACATCAGG 
TCGGCCATCA CTGTGTTCCC CCAGCGGAGC GATGGGAAGC ATGACTTCCG GGTCTGGAAC 
GCCCAGCTCA TCCGCTATGC CGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCC 
GCCAACGTGG AGTTCACACA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC 
TTCGACGTGT TGCCTCTAGT CCTGCAGGCT GACGGCCGTG ACCCAGAGCT CTTCGAAATC 
CCCCCTGACC TTGTTCTCGA GGTGCCCATG GAACATCCCA GGTACGAATG GTTCCGGGAG 
CTGGAGCTCA AGTGGTACGC CCTGCCAGCC GTGGCCAACA TGCTGCTTGA GGTGGGCGGC 
CTCGAGTTCC CAGGGTGCCC CTTCAACGGG TGGTACATGG GCACAGAAGT TGGAGTCCGG 
GACTTCTGCG ACGCCCAGCG ATACAACATC CTGGAGGAAG TGGGCAGAAG GATGGGCCTG 
GAAACGCACA AGGTGGCCTC ACTCTGGAAG GACCGGGCTG TCGTTGAGAT CAACGTCGCT 
GTGCTCCACA GTTTCCAGAA GCAGAACGTG ACCATCATGG ACCACCACTC CGCTGCAGAG 
TCCTTCATGA AGTACATGCA GAATGAGTAC CGCTCCCGAG GGGGCTGCCC AGCTGACTGG 
ATTTGGCTGG TCCCCCCGAT CTCTGGAAGC ATCACCCCCG TGTTCCACCA GGAGATGTTA 
AATTACGTCC TGTCCCCTTT CTACTACTAC CAGGTGGAGC CCTGGAAAAC CCACGTCTGG 
CAGGACGAGA GGCGGAGACC CCAGAGAAGA GAGATTCGAT TCAAAGTCTT GGTCAAAGCC 
GTGTTCTTCG CCTCGGTGCT GATGCATAAG ACCATGGCAT CCCGGGTCAG AGCCACGATC 
CTCTTTGCAA CAGAGACGGG GAGATCGGAA ACGCTGGCCC AGGACCTGGG GGCTTTGTTC 
AGCTGTGCCT TCAACCCCAA GGTTCTCTGC ATGGATCAGT ACCAGCTGAG CCATCTGGAG 
GAGGAGCAGC TGCTGCTGGT GGTGACCAGC ACTTTTGGCA ACGGAGACTC CCCTGGCAAC 
GGAGAGAAAC TGAAGAAGTC CCTCTTGATG CTGAAAGAAC TCACCAACAC GTTCCGGTAC 
GCCGTGTTTG GCCTTGGCTC CAGCATGTAC CCTCAGTTCT GCGCTTTTGC TCATGACATT 
GACCAGAAGT TGTCCCAGCT GGGGGCTTCT CAGCTTGTCC CAACCGGGGA AGGGGACGAA 
CTCAGCGGGC AGGAGGAGGC CTTTCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT 
GAGACATTCG ATGTCAGCGG CAAGCACCAC ATTGAGATCC CCAAGCTCTA CACTTCCAAT 
GTGACCTGGG ACCCGCAGCA CTACAGGCTC GTGCAGGACT CAGAGCCTCT GGACCTCAAC 
AAAGCCCTGA GCAGTATGCA CGCCAAGCAT GTGTTCACCA TGAGGCTCAA ATCGCAGCAG 
AATCTGCAGA GTCCGAAATC CAGCCGCACC ACCCTCCTGG TGGAACTCTC CTGTGAGGGC 
AGCCAAGCTC CAAGCTACCT GCCGGGAGAG CACCTCGGAG TTTTCCCATG CAACCAGCCG 
GCCCTGGTTC AAGGCATCCT TGAGCGAGTG GTGGATGGCC CTGCCCCGCA CCAGCCTGTG 
CGCCTGGAGA CCCTCTGTGA GAATGGCAGC TATTGGGTCA AGGATAAGCG GCTGCCGCCT 
TGCTCACTCA GCCAGGCTCT CACCTATTTC CTGGACATCA CCACCCCGCC AACCCAGCTG 
CTGCTCCGAA AGCTGGCCCA GCTGGCCACA GAAGAGGCTG AGAAGCAGAG GCTGGAGACC 
CTATGCCAGC CCTCAGATTA CAACAAGTGG AAGTTCACCA ACAGCCCCAC ATTTCTGGAG 
GTGCTGGAGG AGTTCCCGTC CCTGCGGGTG TCTGCCAGCT TCCTGCTCTC CCAGCTCCCT 
ATTCTGAAGC CCCGGTACTA CTCCATCAGC TCCTCCAGGG ACCTCACACC CACGGAGATC 
CACCTCACTG TGGCCGTGCT CACGTACCGC ACCCGAGATG GCCAAGGTCC CCTGCACCAT 
GGTGTCTGCA GCACGTGGCT CAGCAGCTTG AAGCCCCAAG ACCCGGTGCC CTGCTTTGTG 
CGAAGTGCCA GCGGCTTCCA GCTTCCCGAG GACCGCTCCC GTCCTTGCAT CCTCATTGGG 
CCCGGCACGG GCATCGCCCC CTTCCGCAGT TTCTGGCAGC AGCGGCTCCA TGAGGCTGAG 
CACAAAGGGC TCCAGGGCGG CCGCATGACC CTGGTTTTCG GGTGCCGCCG CCCAGAAGAG 
GACCACCTCT ACTGGGAGGA GATGCTGGAG ATGGCCCGCA AGGGGGTGCT GCACGAGGTG 
CACACCGCCT ATTCCCGCCT GCCTGACCAG CCCAAGGTCT ATGTTCAAGA CATCCTGCGG 
CAGCGGCTGG CGGGCGAGGT GCTTCGCGTG CTCCATGAGG AGCAAGGTCA CCTCTATGTC 
TGTGGGGATG TGCGCATGGC CCGGGACGTG GCCCGCACCC TGAAGCAGCT GATGGCTACT 
GCGCTGAGCC TGAACGAGGA ACAGGTGGAG GACTATTTCT TCCAGCTCAA GAACCAGAAG 
CGCTATCATG AAGACATCTT TGGTGCTGTA TTTCCCTATG AGGTGAAAAA GGATGGGGCA 
GCAGGGCTGC CCAGCAATCC CAGAGCTCCA GGCGCCCACA GAAGTTAA