NOS2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS2
Entrez Gene ID	455026
Full Name	nitric oxide synthase 2
Synonyms	NOS2A
General protein information	Preferred Names nitric oxide synthase 2 Names nitric oxide synthase, inducible nitric oxide synthase 2, inducible nitric oxide synthase 2A (inducible, hepatocytes)
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016932200.1	XP_016787689.1	nitric oxide synthase, inducible isoform X1
XM_024350675.1	XP_024206443.1	LOW QUALITY PROTEIN: nitric oxide synthase, inducible
XM_009432541.2	XP_009430816.2	nitric oxide synthase, inducible isoform X2
XM_009432543.2	XP_009430818.1	nitric oxide synthase, inducible isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NOS2	NP_001070267.1
Canis lupus familiaris (dog)	NOS2	XP_005624903.1
Xenopus tropicalis (tropical clawed frog)	nos2	XP_002935342.2
Macaca mulatta (Rhesus monkey)	NOS2	XP_001106245.1
Rattus norvegicus (Norway rat)	Nos2	NP_036743.3
Danio rerio (zebrafish)	nos2a	NP_001098407.1
Homo sapiens (human)	NOS2	NP_000616.3
Rattus norvegicus (Norway rat)	LOC497963	XP_006247011.1
Gallus gallus (chicken)	NOS2	NP_990292.1
Pan troglodytes (chimpanzee)	NOS2	XP_001148238.1
Mus musculus (house mouse)	Nos2	NP_035057.1
Danio rerio (zebrafish)	nos2b	NP_001106973.1

Gene Ontology

The following NOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc33486	XM_009432543.2 Latest version!	Pan troglodytes nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPc100158	XM_024350675.1 Latest version!	Pan troglodytes nitric oxide synthase 2 (NOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OPc72820	XM_016932200.1 Latest version!	Pan troglodytes nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPc72821	XM_009432541.2 Latest version!	Pan troglodytes nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS2 ( XM_009432543.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009430818.1 Pan troglodytes nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc33486
Clone ID Related Accession (Same CDS sequence)	XM_009432543.2
Accession Version	XM_009432543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	nitric oxide synthase, inducible isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006484.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009432543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAAC ACATCTGCAG ACACGTGCGT TACTCCACCA ACAATGGCAA CATCAGGTCG 
GCCATCACCG TGTTCCCCCA GCGGAGTGAT GGCAAGCACG ACTTCCGGGT GTGGAATGCT 
CAGCTCATCC GCTATGCTGG CTACCAGATG CCAGATGGCA GCATCAGAGG GGACCCTGCC 
AACGTGGAAT TCACTCAGCT GTGCATCGAC CTGGGCTGGA AGCCCAAGTA CGGCCGCTTC 
GATGTGGTCC CCCTGGTCCT GCAGGCCAAT GGCCGTGACC CTGAGCTCTT CGAAATCCCA 
CCTGACCTTG TGCTTGAGGT GGCCATGGAA CATCCCAAAT ACGAGTGGTT TCGGGAACTG 
GAGCTAAAGT GGTACGCCCT GCCTGCAGTG GCCAACATGC TGCTTGAGGT GGGCGGCCTG 
GAGTTCCCAG GGTGCCCCTT CAATGGCTGG TACATGGGCA CAGAGATCGG AGTCCGGGAC 
TTCTGTGACG TCCAGCGCTA CAACATCCTG GAGGAAGTGG GCAGGAGAAT GGGCTTGGAA 
ACGCACAAGC TGGCCTCGCT CTGGAAAGAC CAGGCTGTCG TTGAGATCAA CATTGCTGTG 
CTCCATAGTT TCCAGAAGCA GAATGTGACC ATCATGGACC ACCACTCGGC TGCAGAATCC 
TTCATGAAGT ACATGCAGAA TGAATACCGG TCCCGTGGGG GCTGCCCGGC AGACTGGATT 
TGGCTGGTCC CTCCCATGTC TGGGAGCATC ACCCCCGTGT TTCACCAGGA GATGCTGAAC 
TACGTCCTGT CCCCTTTCTA CTACTATCAG GTAGAGGCCT GGAAAACCCA TGTCTGGCAG 
GACGAGAAGC GGAGACCCAA GAGAAGAGAG ATTCCATTGA AAGTCTTGGT CAAAGCTGTG 
CTCTTTGCCT GTATGCTGAT GCGCAAGACA ATGGCGTCCC GAGTCAGAGT CACCATCCTC 
TTTGCGACAG AGACAGGAAA ATCAGAGGCG CTGGCCTGGG ACCTGGGGGC CTTGTTCAGC 
TGTGCCTTCA ACCCCAAGGT TGTCTGCATG GATAAGTACA GGCTGAGCTG CCTGGAGGAG 
GAGCGGCTGC TGTTGGTGGT GACCAGTACG TTTGGCAATG GAGACTGCCC CGGCAATGGA 
GAGAAACTGA AGAAATCGCT CTTCATGCTG AAAGAGCTCA ACAACAAATT CAGGTACGCT 
GTGTTTGGCC TCGGCTCCAG CATGTACCCT CGGTTCTGCG CCTTTGCTCA TGACATTGAT 
CAGAAGCTGT CCCACCTGGG GGCCTCTCAG CTCACCCCGA TGGGAGAAGG GGATGAGCTC 
AGTGGGCAGG AGGACGCCTT CCGCAGCTGG GCCGTGCAAA CCTTCAAGGC AGCCTGTGAG 
ATGTTTGATG TCCGAGGCAA ACAGCACATT CAGATCCCCA AGCTCTACAC CTCCAATGTG 
ACCTGGGACC CGCACCACTA CAGGCTCGTG CAGGACTCAC AGCCTTTGGA CCTCAGCAAA 
GCCCTCAGCA GCATGCATGC CAAGAACGTG TTCACCATGA GGCTCAAATC TCGGCAGAAT 
CTACAAAGTC CGACATCCAG CCGTGCCACC ATCCTGGTGG AACTCTCCTG TGAGGATGGC 
CAAGGCCTGA ACTACCTGCC GGGGGAGCAC CTTGGGGTTT GCCCAGGCAA CCAGCCGGCC 
CTGGTCCAAG GCATCCTGGA GCGAGTGGTG GATGGCCCCA CACCCCACCA GACAGTGCAC 
CTGGAGGCCC TGGATGAGAG TGGCAGCTAC TGGGTCAGTG ACAAGAGGCT GCCCCCCTGC 
TCACTCAGCC AGGCCCTCAC CTACTTCCTG GACATCACAA CACCCCCAAC CCAGCTGCTG 
CTCCTAAAGC TGGCCCAGGT GGCCACAGAA GCGACTGAGA GACAGAGGCT GGAGGCCCTT 
TGCCAGCCCT CAGAGTACAG CAAGTGGAAG TTCACCAACA GCCCCACATT CCTGGAGGTG 
CTAGAGGAGT TCCCGTCCCT GCGGGTGTCT GCTGGCTTCC TGCTTTCCCA GCTCCCCATT 
CTGAAGCCCA GGTTCTACTC CATCAGCTCC TCCCGGGATC ACACGCCCAC GGAGATCCAC 
CTGACTGTGG CCGTGGTCAC CTACCACACC CGAGATGGCC AGGGTCCCCT GCACCACGGC 
GTCTGCAGCA CTTGGCTCAA CAGCCTGAAG CCCCAAGACC CAGTGCCCTG CTTTGTGCGG 
AATGCCAGCG GCTTCCACCT CCCCGAGGAT CCCTCCCATC CTTGCATCCT CATCGGGCCT 
GGCACAGGCA TCGCGCCCTT CCGCAGTTTC TGGCAGCAAC GGCTCCATGA CTCCCAGCAC 
AAGGGAGTGC GGGGAGGCCG CATGACCTTG GTGTTTGGGT GCCGCCGCCC AGATGAGGAC 
CACATCTACC AGGAGGAGAT GCTGGAGATG GCCCAGAAGG GGGTGCTGCA TGCGGTGCAC 
ACAGCCTATT CCCGCCTGCC TGGCAAGCCC AAGGTCTATG TTCAGGACAT CCTGCGGCAG 
CAGCTGGCCA GCGAGGTGCT CCGTGTGCTC CACAAGGAGC CAGGCCACCT CTATGTTTGC 
GGGGATGTGC GCATGGCCCG GGACGTGGCC CACACCCTGA AGCAGCTGGT GGCTGCCAAG 
CTGAAATTGA ATGAGGAGCA GGTCGAGGAC TATTTCTTTC AGCTCAAGAG CCAGAAGCGC 
TATCACGAAG ATATCTTTGG TGCTGTATTT CCCTACGAGG CGAAGAAGGA CAGGGTGGCG 
GTGCAGCCCA GCAACCTGGA GATGTCAGCG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009430818.1
CDS	528..3323
Translation	MFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACEMFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVHLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLLKLAQVATEATERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSNLEMSAL

Target ORF information:

RefSeq Version	XM_009432543.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes nitric oxide synthase 2 (NOS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009432543.2

ORF Insert Sequence:

ATGTTTGAAC ACATCTGCAG ACACGTGCGT TACTCCACCA ACAATGGCAA CATCAGGTCG 
GCCATCACCG TGTTCCCCCA GCGGAGTGAT GGCAAGCACG ACTTCCGGGT GTGGAATGCT 
CAGCTCATCC GCTATGCTGG CTACCAGATG CCAGATGGCA GCATCAGAGG GGACCCTGCC 
AACGTGGAAT TCACTCAGCT GTGCATCGAC CTGGGCTGGA AGCCCAAGTA CGGCCGCTTC 
GATGTGGTCC CCCTGGTCCT GCAGGCCAAT GGCCGTGACC CTGAGCTCTT CGAAATCCCA 
CCTGACCTTG TGCTTGAGGT GGCCATGGAA CATCCCAAAT ACGAGTGGTT TCGGGAACTG 
GAGCTAAAGT GGTACGCCCT GCCTGCAGTG GCCAACATGC TGCTTGAGGT GGGCGGCCTG 
GAGTTCCCAG GGTGCCCCTT CAATGGCTGG TACATGGGCA CAGAGATCGG AGTCCGGGAC 
TTCTGTGACG TCCAGCGCTA CAACATCCTG GAGGAAGTGG GCAGGAGAAT GGGCTTGGAA 
ACGCACAAGC TGGCCTCGCT CTGGAAAGAC CAGGCTGTCG TTGAGATCAA CATTGCTGTG 
CTCCATAGTT TCCAGAAGCA GAATGTGACC ATCATGGACC ACCACTCGGC TGCAGAATCC 
TTCATGAAGT ACATGCAGAA TGAATACCGG TCCCGTGGGG GCTGCCCGGC AGACTGGATT 
TGGCTGGTCC CTCCCATGTC TGGGAGCATC ACCCCCGTGT TTCACCAGGA GATGCTGAAC 
TACGTCCTGT CCCCTTTCTA CTACTATCAG GTAGAGGCCT GGAAAACCCA TGTCTGGCAG 
GACGAGAAGC GGAGACCCAA GAGAAGAGAG ATTCCATTGA AAGTCTTGGT CAAAGCTGTG 
CTCTTTGCCT GTATGCTGAT GCGCAAGACA ATGGCGTCCC GAGTCAGAGT CACCATCCTC 
TTTGCGACAG AGACAGGAAA ATCAGAGGCG CTGGCCTGGG ACCTGGGGGC CTTGTTCAGC 
TGTGCCTTCA ACCCCAAGGT TGTCTGCATG GATAAGTACA GGCTGAGCTG CCTGGAGGAG 
GAGCGGCTGC TGTTGGTGGT GACCAGTACG TTTGGCAATG GAGACTGCCC CGGCAATGGA 
GAGAAACTGA AGAAATCGCT CTTCATGCTG AAAGAGCTCA ACAACAAATT CAGGTACGCT 
GTGTTTGGCC TCGGCTCCAG CATGTACCCT CGGTTCTGCG CCTTTGCTCA TGACATTGAT 
CAGAAGCTGT CCCACCTGGG GGCCTCTCAG CTCACCCCGA TGGGAGAAGG GGATGAGCTC 
AGTGGGCAGG AGGACGCCTT CCGCAGCTGG GCCGTGCAAA CCTTCAAGGC AGCCTGTGAG 
ATGTTTGATG TCCGAGGCAA ACAGCACATT CAGATCCCCA AGCTCTACAC CTCCAATGTG 
ACCTGGGACC CGCACCACTA CAGGCTCGTG CAGGACTCAC AGCCTTTGGA CCTCAGCAAA 
GCCCTCAGCA GCATGCATGC CAAGAACGTG TTCACCATGA GGCTCAAATC TCGGCAGAAT 
CTACAAAGTC CGACATCCAG CCGTGCCACC ATCCTGGTGG AACTCTCCTG TGAGGATGGC 
CAAGGCCTGA ACTACCTGCC GGGGGAGCAC CTTGGGGTTT GCCCAGGCAA CCAGCCGGCC 
CTGGTCCAAG GCATCCTGGA GCGAGTGGTG GATGGCCCCA CACCCCACCA GACAGTGCAC 
CTGGAGGCCC TGGATGAGAG TGGCAGCTAC TGGGTCAGTG ACAAGAGGCT GCCCCCCTGC 
TCACTCAGCC AGGCCCTCAC CTACTTCCTG GACATCACAA CACCCCCAAC CCAGCTGCTG 
CTCCTAAAGC TGGCCCAGGT GGCCACAGAA GCGACTGAGA GACAGAGGCT GGAGGCCCTT 
TGCCAGCCCT CAGAGTACAG CAAGTGGAAG TTCACCAACA GCCCCACATT CCTGGAGGTG 
CTAGAGGAGT TCCCGTCCCT GCGGGTGTCT GCTGGCTTCC TGCTTTCCCA GCTCCCCATT 
CTGAAGCCCA GGTTCTACTC CATCAGCTCC TCCCGGGATC ACACGCCCAC GGAGATCCAC 
CTGACTGTGG CCGTGGTCAC CTACCACACC CGAGATGGCC AGGGTCCCCT GCACCACGGC 
GTCTGCAGCA CTTGGCTCAA CAGCCTGAAG CCCCAAGACC CAGTGCCCTG CTTTGTGCGG 
AATGCCAGCG GCTTCCACCT CCCCGAGGAT CCCTCCCATC CTTGCATCCT CATCGGGCCT 
GGCACAGGCA TCGCGCCCTT CCGCAGTTTC TGGCAGCAAC GGCTCCATGA CTCCCAGCAC 
AAGGGAGTGC GGGGAGGCCG CATGACCTTG GTGTTTGGGT GCCGCCGCCC AGATGAGGAC 
CACATCTACC AGGAGGAGAT GCTGGAGATG GCCCAGAAGG GGGTGCTGCA TGCGGTGCAC 
ACAGCCTATT CCCGCCTGCC TGGCAAGCCC AAGGTCTATG TTCAGGACAT CCTGCGGCAG 
CAGCTGGCCA GCGAGGTGCT CCGTGTGCTC CACAAGGAGC CAGGCCACCT CTATGTTTGC 
GGGGATGTGC GCATGGCCCG GGACGTGGCC CACACCCTGA AGCAGCTGGT GGCTGCCAAG 
CTGAAATTGA ATGAGGAGCA GGTCGAGGAC TATTTCTTTC AGCTCAAGAG CCAGAAGCGC 
TATCACGAAG ATATCTTTGG TGCTGTATTT CCCTACGAGG CGAAGAAGGA CAGGGTGGCG 
GTGCAGCCCA GCAACCTGGA GATGTCAGCG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_024350675.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024206443.1 Pan troglodytes nitric oxide synthase 2 (NOS2), mRNA.

Price & Availability↑

CloneID	OPc100158
Clone ID Related Accession (Same CDS sequence)	XM_024350675.1
Accession Version	XM_024350675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1521388800000
Organism	Pan troglodytes(chimpanzee)
Product	LOW QUALITY PROTEIN: nitric oxide synthase, inducible
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036896.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG ACCAGTGACA 
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC 
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC 
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT 
CACCATAAAG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG 
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC 
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACCGCC 
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG 
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA 
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG 
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC 
TTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT 
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA 
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC 
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG 
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC 
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA 
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC 
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG 
GAGGAGGAGC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCCGGC 
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG 
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC 
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT 
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGATGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC 
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC 
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG 
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG 
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG 
CCGGCCCTGG TCCAAGGCAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA 
GTGCACCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC 
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACAACACC CCCAACCCAG 
CTGCTGCTCC TAAAGCTGGC CCAGGTGGCC ACAGAAGCGA CTGAGAGACA GAGGCTGGAG 
GCCCTTTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG 
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC 
CCCATTCTGA AGCCCAGGTT TTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACGGAG 
ATCCACCTGA CTGTGGCCGT GCTCACGTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC 
CACGGCGTCT GCAGCACTTG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT 
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC 
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT 
GAGGACCACC CCTACCGGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG 
GTGCACACGN CCTATTCCCG CCTGCCTGGC AAGCTAAGGC CCCTCGCCTC CCCGAGCCTT 
CACCTCCTGA GCTCGAAAAT GCTGGTAATT CACAACCATC AGAGTGATGC TGTGAAGAGC 
GTTACACCCT CTCATACAGC AGAAGCGACA GACCTGGAGA GGTTCAGGAC TAACCTAGGT 
CACACGCGGG TTAACCCCAG AGCCCAGCCT GCTGAAGGTC CCCAGCAGGC CCCCTGGGAC 
ACGCGACTGG CCGGGGAGAG GTGGTCCAGG CTGGCTACGC TCAAGCCTTG CAAGGCTGGT 
GGGCGGCCGA TGCTTCCACC ACGGCCATCA GAGGGGCTCT TCTGTCCCCA GGGCCGCGTC 
TATGTTCAGG ACATCCTGCG GCAGCAGCTG GCCAGCGAGG TGCTCCGTGT GCTCCACAAG 
GAGCCAGGCC ACCTCTATGT TTGCGGGGAT GTGCGCATGG CCTGGGACGT GGCCCACACC 
CTGAAGCAGC TGGTGGCTGC CAAGCTGAAA TTGAATGAGG AGCAGGTCGA GGACTATTTC 
TTTCAGCTCA AGAGCCAGAA GCGCTATCAC GAAGATATCT TTGGTGCTGT ATTTCCCTAC 
GAGGCGAAGA AGGACAGGGT GGCGGTGCAG CCCAGCAACC TGGAGATGTC AGCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024206443.1
CDS	263..4042
Translation	MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSRPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACEMFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVHLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLLKLAQVATEATERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVLTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHPYREEMLEMAQKGVLHAVHTnullYSRLPGKLRPLASPSLHLLSSKMLVIHNHQSDAVKSVTPSHTAEATDLERFRTNLGHTRVNPRAQPAEGPQQAPWDTRLAGERWSRLATLKPCKAGGRPMLPPRPSEGLFCPQGRVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMAWDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSNLEMSAL

Target ORF information:

RefSeq Version	XM_024350675.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes nitric oxide synthase 2 (NOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024350675.1

ORF Insert Sequence:

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG ACCAGTGACA 
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC 
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC 
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT 
CACCATAAAG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG 
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC 
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACCGCC 
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG 
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA 
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG 
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC 
TTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT 
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA 
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC 
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG 
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC 
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA 
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC 
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG 
GAGGAGGAGC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCCGGC 
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG 
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC 
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT 
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGATGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC 
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC 
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG 
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG 
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG 
CCGGCCCTGG TCCAAGGCAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA 
GTGCACCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC 
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACAACACC CCCAACCCAG 
CTGCTGCTCC TAAAGCTGGC CCAGGTGGCC ACAGAAGCGA CTGAGAGACA GAGGCTGGAG 
GCCCTTTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG 
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC 
CCCATTCTGA AGCCCAGGTT TTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACGGAG 
ATCCACCTGA CTGTGGCCGT GCTCACGTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC 
CACGGCGTCT GCAGCACTTG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT 
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC 
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT 
GAGGACCACC CCTACCGGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG 
GTGCACACGN CCTATTCCCG CCTGCCTGGC AAGCTAAGGC CCCTCGCCTC CCCGAGCCTT 
CACCTCCTGA GCTCGAAAAT GCTGGTAATT CACAACCATC AGAGTGATGC TGTGAAGAGC 
GTTACACCCT CTCATACAGC AGAAGCGACA GACCTGGAGA GGTTCAGGAC TAACCTAGGT 
CACACGCGGG TTAACCCCAG AGCCCAGCCT GCTGAAGGTC CCCAGCAGGC CCCCTGGGAC 
ACGCGACTGG CCGGGGAGAG GTGGTCCAGG CTGGCTACGC TCAAGCCTTG CAAGGCTGGT 
GGGCGGCCGA TGCTTCCACC ACGGCCATCA GAGGGGCTCT TCTGTCCCCA GGGCCGCGTC 
TATGTTCAGG ACATCCTGCG GCAGCAGCTG GCCAGCGAGG TGCTCCGTGT GCTCCACAAG 
GAGCCAGGCC ACCTCTATGT TTGCGGGGAT GTGCGCATGG CCTGGGACGT GGCCCACACC 
CTGAAGCAGC TGGTGGCTGC CAAGCTGAAA TTGAATGAGG AGCAGGTCGA GGACTATTTC 
TTTCAGCTCA AGAGCCAGAA GCGCTATCAC GAAGATATCT TTGGTGCTGT ATTTCCCTAC 
GAGGCGAAGA AGGACAGGGT GGCGGTGCAG CCCAGCAACC TGGAGATGTC AGCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_016932200.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016787689.1 Pan troglodytes nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

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CloneID	OPc72820
Clone ID Related Accession (Same CDS sequence)	XM_016932200.1
Accession Version	XM_016932200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	nitric oxide synthase, inducible isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006484.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA 
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC 
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC 
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT 
CACCATAAAG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG 
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC 
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACCGCC 
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG 
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA 
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG 
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC 
TTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT 
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA 
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC 
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG 
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC 
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA 
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC 
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG 
GAGGAGGAGC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCCGGC 
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG 
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC 
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT 
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGATGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC 
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC 
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG 
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG 
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG 
CCGGCCCTGG TCCAAGGCAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA 
GTGCACCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC 
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACAACACC CCCAACCCAG 
CTGCTGCTCC TAAAGCTGGC CCAGGTGGCC ACAGAAGCGA CTGAGAGACA GAGGCTGGAG 
GCCCTTTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG 
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC 
CCCATTCTGA AGCCCAGGTT CTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACGGAG 
ATCCACCTGA CTGTGGCCGT GGTCACCTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC 
CACGGCGTCT GCAGCACTTG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT 
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC 
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT 
GAGGACCACA TCTACCAGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG 
GTGCACACAG CCTATTCCCG CCTGCCTGGC AAGCCCAAGG TCTATGTTCA GGACATCCTG 
CGGCAGCAGC TGGCCAGCGA GGTGCTCCGT GTGCTCCACA AGGAGCCAGG CCACCTCTAT 
GTTTGCGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCACA CCCTGAAGCA GCTGGTGGCT 
GCCAAGCTGA AATTGAATGA GGAGCAGGTC GAGGACTATT TCTTTCAGCT CAAGAGCCAG 
AAGCGCTATC ACGAAGATAT CTTTGGTGCT GTATTTCCCT ACGAGGCGAA GAAGGACAGG 
GTGGCGGTGC AGCCCAGCAA CCTGGAGATG TCAGCGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016787689.1
CDS	265..3726
Translation	MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACEMFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVHLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLLKLAQVATEATERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSNLEMSAL

Target ORF information:

RefSeq Version	XM_016932200.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes nitric oxide synthase 2 (NOS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016932200.1

ORF Insert Sequence:

ATGGCCTGTC CTTGGAAATT TCTGTTCAAG ACCAAATTCC ACCAGTATGC AATGAATGGG 
GAAAAAGACA TCAACAACAA TGTGGAGAAA GCCCCCTGTG CCACCTCCAG TCCAGTGACA 
CAGGATGACC TTCAGTATCA CAACCTCAGC AAGCAGCAGA ATGAGTCCCC GCAGCCCCTC 
GTGGAGACGG GAAAGAAGTC TCCAGAATCT CTGGTCAAGC TGGATGCAAC CCCATTGTCC 
TCCCCACGGC ATGTGAGGAT CAAAAACTGG GGCAGCGGGA TGACTTTCCA AGACACACTT 
CACCATAAAG CCAAAGGGAT TTTAACTTGC AGGTCCAAAT CTTGCCTGGG GTCCATTATG 
ACTCCCAAAA GTTTGACCAG AGGACCCAGG GACAAGCCTA CCCCTCCAGA TGAGCTTCTA 
CCTCAAGCTA TCGAATTTGT CAACCAATAT TACGGCTCCT TCAAAGAGGC AAAAATAGAG 
GAACATCTGG CCAGGGTGGA AGCGGTAACA AAGGAGATAG AAACAACAGG AACCTACCAA 
CTGACGGGAG ATGAGCTCAT CTTCGCCACC AAGCAGGCCT GGCGCAATGC CCCACGCTGC 
ATTGGGAGGA TCCAGTGGTC CAACCTGCAG GTCTTCGATG CCCGCAGCTG TTCCACCGCC 
CGGGAAATGT TTGAACACAT CTGCAGACAC GTGCGTTACT CCACCAACAA TGGCAACATC 
AGGTCGGCCA TCACCGTGTT CCCCCAGCGG AGTGATGGCA AGCACGACTT CCGGGTGTGG 
AATGCTCAGC TCATCCGCTA TGCTGGCTAC CAGATGCCAG ATGGCAGCAT CAGAGGGGAC 
CCTGCCAACG TGGAATTCAC TCAGCTGTGC ATCGACCTGG GCTGGAAGCC CAAGTACGGC 
CGCTTCGATG TGGTCCCCCT GGTCCTGCAG GCCAATGGCC GTGACCCTGA GCTCTTCGAA 
ATCCCACCTG ACCTTGTGCT TGAGGTGGCC ATGGAACATC CCAAATACGA GTGGTTTCGG 
GAACTGGAGC TAAAGTGGTA CGCCCTGCCT GCAGTGGCCA ACATGCTGCT TGAGGTGGGC 
GGCCTGGAGT TCCCAGGGTG CCCCTTCAAT GGCTGGTACA TGGGCACAGA GATCGGAGTC 
CGGGACTTCT GTGACGTCCA GCGCTACAAC ATCCTGGAGG AAGTGGGCAG GAGAATGGGC 
TTGGAAACGC ACAAGCTGGC CTCGCTCTGG AAAGACCAGG CTGTCGTTGA GATCAACATT 
GCTGTGCTCC ATAGTTTCCA GAAGCAGAAT GTGACCATCA TGGACCACCA CTCGGCTGCA 
GAATCCTTCA TGAAGTACAT GCAGAATGAA TACCGGTCCC GTGGGGGCTG CCCGGCAGAC 
TGGATTTGGC TGGTCCCTCC CATGTCTGGG AGCATCACCC CCGTGTTTCA CCAGGAGATG 
CTGAACTACG TCCTGTCCCC TTTCTACTAC TATCAGGTAG AGGCCTGGAA AACCCATGTC 
TGGCAGGACG AGAAGCGGAG ACCCAAGAGA AGAGAGATTC CATTGAAAGT CTTGGTCAAA 
GCTGTGCTCT TTGCCTGTAT GCTGATGCGC AAGACAATGG CGTCCCGAGT CAGAGTCACC 
ATCCTCTTTG CGACAGAGAC AGGAAAATCA GAGGCGCTGG CCTGGGACCT GGGGGCCTTG 
TTCAGCTGTG CCTTCAACCC CAAGGTTGTC TGCATGGATA AGTACAGGCT GAGCTGCCTG 
GAGGAGGAGC GGCTGCTGTT GGTGGTGACC AGTACGTTTG GCAATGGAGA CTGCCCCGGC 
AATGGAGAGA AACTGAAGAA ATCGCTCTTC ATGCTGAAAG AGCTCAACAA CAAATTCAGG 
TACGCTGTGT TTGGCCTCGG CTCCAGCATG TACCCTCGGT TCTGCGCCTT TGCTCATGAC 
ATTGATCAGA AGCTGTCCCA CCTGGGGGCC TCTCAGCTCA CCCCGATGGG AGAAGGGGAT 
GAGCTCAGTG GGCAGGAGGA CGCCTTCCGC AGCTGGGCCG TGCAAACCTT CAAGGCAGCC 
TGTGAGATGT TTGATGTCCG AGGCAAACAG CACATTCAGA TCCCCAAGCT CTACACCTCC 
AATGTGACCT GGGACCCGCA CCACTACAGG CTCGTGCAGG ACTCACAGCC TTTGGACCTC 
AGCAAAGCCC TCAGCAGCAT GCATGCCAAG AACGTGTTCA CCATGAGGCT CAAATCTCGG 
CAGAATCTAC AAAGTCCGAC ATCCAGCCGT GCCACCATCC TGGTGGAACT CTCCTGTGAG 
GATGGCCAAG GCCTGAACTA CCTGCCGGGG GAGCACCTTG GGGTTTGCCC AGGCAACCAG 
CCGGCCCTGG TCCAAGGCAT CCTGGAGCGA GTGGTGGATG GCCCCACACC CCACCAGACA 
GTGCACCTGG AGGCCCTGGA TGAGAGTGGC AGCTACTGGG TCAGTGACAA GAGGCTGCCC 
CCCTGCTCAC TCAGCCAGGC CCTCACCTAC TTCCTGGACA TCACAACACC CCCAACCCAG 
CTGCTGCTCC TAAAGCTGGC CCAGGTGGCC ACAGAAGCGA CTGAGAGACA GAGGCTGGAG 
GCCCTTTGCC AGCCCTCAGA GTACAGCAAG TGGAAGTTCA CCAACAGCCC CACATTCCTG 
GAGGTGCTAG AGGAGTTCCC GTCCCTGCGG GTGTCTGCTG GCTTCCTGCT TTCCCAGCTC 
CCCATTCTGA AGCCCAGGTT CTACTCCATC AGCTCCTCCC GGGATCACAC GCCCACGGAG 
ATCCACCTGA CTGTGGCCGT GGTCACCTAC CACACCCGAG ATGGCCAGGG TCCCCTGCAC 
CACGGCGTCT GCAGCACTTG GCTCAACAGC CTGAAGCCCC AAGACCCAGT GCCCTGCTTT 
GTGCGGAATG CCAGCGGCTT CCACCTCCCC GAGGATCCCT CCCATCCTTG CATCCTCATC 
GGGCCTGGCA CAGGCATCGC GCCCTTCCGC AGTTTCTGGC AGCAACGGCT CCATGACTCC 
CAGCACAAGG GAGTGCGGGG AGGCCGCATG ACCTTGGTGT TTGGGTGCCG CCGCCCAGAT 
GAGGACCACA TCTACCAGGA GGAGATGCTG GAGATGGCCC AGAAGGGGGT GCTGCATGCG 
GTGCACACAG CCTATTCCCG CCTGCCTGGC AAGCCCAAGG TCTATGTTCA GGACATCCTG 
CGGCAGCAGC TGGCCAGCGA GGTGCTCCGT GTGCTCCACA AGGAGCCAGG CCACCTCTAT 
GTTTGCGGGG ATGTGCGCAT GGCCCGGGAC GTGGCCCACA CCCTGAAGCA GCTGGTGGCT 
GCCAAGCTGA AATTGAATGA GGAGCAGGTC GAGGACTATT TCTTTCAGCT CAAGAGCCAG 
AAGCGCTATC ACGAAGATAT CTTTGGTGCT GTATTTCCCT ACGAGGCGAA GAAGGACAGG 
GTGGCGGTGC AGCCCAGCAA CCTGGAGATG TCAGCGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS2 ( XM_009432541.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009430816.2 Pan troglodytes nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc72821
Clone ID Related Accession (Same CDS sequence)	XM_009432541.2
Accession Version	XM_009432541.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1464710400000
Organism	Pan troglodytes(chimpanzee)
Product	nitric oxide synthase, inducible isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006484.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009432541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGAGG GGCTGGGCGC GGCGTGCGAG GCCGCCGGGC CCCGGCTGTG TAGGCTGCTC 
TGCAGGATCC TCCGGCCGAA GCCTGACTGC TGCCCGCGCA GAGCCAGTCC AGTGACACAG 
GATGACCTTC AGTATCACAA CCTCAGCAAG CAGCAGAATG AGTCCCCGCA GCCCCTCGTG 
GAGACGGGAA AGAAGTCTCC AGAATCTCTG GTCAAGCTGG ATGCAACCCC ATTGTCCTCC 
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGGATGA CTTTCCAAGA CACACTTCAC 
CATAAAGCCA AAGGGATTTT AACTTGCAGG TCCAAATCTT GCCTGGGGTC CATTATGACT 
CCCAAAAGTT TGACCAGAGG ACCCAGGGAC AAGCCTACCC CTCCAGATGA GCTTCTACCT 
CAAGCTATCG AATTTGTCAA CCAATATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA 
CATCTGGCCA GGGTGGAAGC GGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAACTG 
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAATGCCCC ACGCTGCATT 
GGGAGGATCC AGTGGTCCAA CCTGCAGGTC TTCGATGCCC GCAGCTGTTC CACCGCCCGG 
GAAATGTTTG AACACATCTG CAGACACGTG CGTTACTCCA CCAACAATGG CAACATCAGG 
TCGGCCATCA CCGTGTTCCC CCAGCGGAGT GATGGCAAGC ACGACTTCCG GGTGTGGAAT 
GCTCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCT 
GCCAACGTGG AATTCACTCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC 
TTCGATGTGG TCCCCCTGGT CCTGCAGGCC AATGGCCGTG ACCCTGAGCT CTTCGAAATC 
CCACCTGACC TTGTGCTTGA GGTGGCCATG GAACATCCCA AATACGAGTG GTTTCGGGAA 
CTGGAGCTAA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGCGGC 
CTGGAGTTCC CAGGGTGCCC CTTCAATGGC TGGTACATGG GCACAGAGAT CGGAGTCCGG 
GACTTCTGTG ACGTCCAGCG CTACAACATC CTGGAGGAAG TGGGCAGGAG AATGGGCTTG 
GAAACGCACA AGCTGGCCTC GCTCTGGAAA GACCAGGCTG TCGTTGAGAT CAACATTGCT 
GTGCTCCATA GTTTCCAGAA GCAGAATGTG ACCATCATGG ACCACCACTC GGCTGCAGAA 
TCCTTCATGA AGTACATGCA GAATGAATAC CGGTCCCGTG GGGGCTGCCC GGCAGACTGG 
ATTTGGCTGG TCCCTCCCAT GTCTGGGAGC ATCACCCCCG TGTTTCACCA GGAGATGCTG 
AACTACGTCC TGTCCCCTTT CTACTACTAT CAGGTAGAGG CCTGGAAAAC CCATGTCTGG 
CAGGACGAGA AGCGGAGACC CAAGAGAAGA GAGATTCCAT TGAAAGTCTT GGTCAAAGCT 
GTGCTCTTTG CCTGTATGCT GATGCGCAAG ACAATGGCGT CCCGAGTCAG AGTCACCATC 
CTCTTTGCGA CAGAGACAGG AAAATCAGAG GCGCTGGCCT GGGACCTGGG GGCCTTGTTC 
AGCTGTGCCT TCAACCCCAA GGTTGTCTGC ATGGATAAGT ACAGGCTGAG CTGCCTGGAG 
GAGGAGCGGC TGCTGTTGGT GGTGACCAGT ACGTTTGGCA ATGGAGACTG CCCCGGCAAT 
GGAGAGAAAC TGAAGAAATC GCTCTTCATG CTGAAAGAGC TCAACAACAA ATTCAGGTAC 
GCTGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCGGTTCT GCGCCTTTGC TCATGACATT 
GATCAGAAGC TGTCCCACCT GGGGGCCTCT CAGCTCACCC CGATGGGAGA AGGGGATGAG 
CTCAGTGGGC AGGAGGACGC CTTCCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT 
GAGATGTTTG ATGTCCGAGG CAAACAGCAC ATTCAGATCC CCAAGCTCTA CACCTCCAAT 
GTGACCTGGG ACCCGCACCA CTACAGGCTC GTGCAGGACT CACAGCCTTT GGACCTCAGC 
AAAGCCCTCA GCAGCATGCA TGCCAAGAAC GTGTTCACCA TGAGGCTCAA ATCTCGGCAG 
AATCTACAAA GTCCGACATC CAGCCGTGCC ACCATCCTGG TGGAACTCTC CTGTGAGGAT 
GGCCAAGGCC TGAACTACCT GCCGGGGGAG CACCTTGGGG TTTGCCCAGG CAACCAGCCG 
GCCCTGGTCC AAGGCATCCT GGAGCGAGTG GTGGATGGCC CCACACCCCA CCAGACAGTG 
CACCTGGAGG CCCTGGATGA GAGTGGCAGC TACTGGGTCA GTGACAAGAG GCTGCCCCCC 
TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CAACACCCCC AACCCAGCTG 
CTGCTCCTAA AGCTGGCCCA GGTGGCCACA GAAGCGACTG AGAGACAGAG GCTGGAGGCC 
CTTTGCCAGC CCTCAGAGTA CAGCAAGTGG AAGTTCACCA ACAGCCCCAC ATTCCTGGAG 
GTGCTAGAGG AGTTCCCGTC CCTGCGGGTG TCTGCTGGCT TCCTGCTTTC CCAGCTCCCC 
ATTCTGAAGC CCAGGTTCTA CTCCATCAGC TCCTCCCGGG ATCACACGCC CACGGAGATC 
CACCTGACTG TGGCCGTGGT CACCTACCAC ACCCGAGATG GCCAGGGTCC CCTGCACCAC 
GGCGTCTGCA GCACTTGGCT CAACAGCCTG AAGCCCCAAG ACCCAGTGCC CTGCTTTGTG 
CGGAATGCCA GCGGCTTCCA CCTCCCCGAG GATCCCTCCC ATCCTTGCAT CCTCATCGGG 
CCTGGCACAG GCATCGCGCC CTTCCGCAGT TTCTGGCAGC AACGGCTCCA TGACTCCCAG 
CACAAGGGAG TGCGGGGAGG CCGCATGACC TTGGTGTTTG GGTGCCGCCG CCCAGATGAG 
GACCACATCT ACCAGGAGGA GATGCTGGAG ATGGCCCAGA AGGGGGTGCT GCATGCGGTG 
CACACAGCCT ATTCCCGCCT GCCTGGCAAG CCCAAGGTCT ATGTTCAGGA CATCCTGCGG 
CAGCAGCTGG CCAGCGAGGT GCTCCGTGTG CTCCACAAGG AGCCAGGCCA CCTCTATGTT 
TGCGGGGATG TGCGCATGGC CCGGGACGTG GCCCACACCC TGAAGCAGCT GGTGGCTGCC 
AAGCTGAAAT TGAATGAGGA GCAGGTCGAG GACTATTTCT TTCAGCTCAA GAGCCAGAAG 
CGCTATCACG AAGATATCTT TGGTGCTGTA TTTCCCTACG AGGCGAAGAA GGACAGGGTG 
GCGGTGCAGC CCAGCAACCT GGAGATGTCA GCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009430816.2
CDS	21..3479
Translation	MREGLGAACEAAGPRLCRLLCRILRPKPDCCPRRASPVTQDDLQYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACEMFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPHQTVHLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLLKLAQVATEATERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSRDHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPSNLEMSAL

Target ORF information:

RefSeq Version	XM_009432541.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes nitric oxide synthase 2 (NOS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009432541.2

ORF Insert Sequence:

ATGCGCGAGG GGCTGGGCGC GGCGTGCGAG GCCGCCGGGC CCCGGCTGTG TAGGCTGCTC 
TGCAGGATCC TCCGGCCGAA GCCTGACTGC TGCCCGCGCA GAGCCAGTCC AGTGACACAG 
GATGACCTTC AGTATCACAA CCTCAGCAAG CAGCAGAATG AGTCCCCGCA GCCCCTCGTG 
GAGACGGGAA AGAAGTCTCC AGAATCTCTG GTCAAGCTGG ATGCAACCCC ATTGTCCTCC 
CCACGGCATG TGAGGATCAA AAACTGGGGC AGCGGGATGA CTTTCCAAGA CACACTTCAC 
CATAAAGCCA AAGGGATTTT AACTTGCAGG TCCAAATCTT GCCTGGGGTC CATTATGACT 
CCCAAAAGTT TGACCAGAGG ACCCAGGGAC AAGCCTACCC CTCCAGATGA GCTTCTACCT 
CAAGCTATCG AATTTGTCAA CCAATATTAC GGCTCCTTCA AAGAGGCAAA AATAGAGGAA 
CATCTGGCCA GGGTGGAAGC GGTAACAAAG GAGATAGAAA CAACAGGAAC CTACCAACTG 
ACGGGAGATG AGCTCATCTT CGCCACCAAG CAGGCCTGGC GCAATGCCCC ACGCTGCATT 
GGGAGGATCC AGTGGTCCAA CCTGCAGGTC TTCGATGCCC GCAGCTGTTC CACCGCCCGG 
GAAATGTTTG AACACATCTG CAGACACGTG CGTTACTCCA CCAACAATGG CAACATCAGG 
TCGGCCATCA CCGTGTTCCC CCAGCGGAGT GATGGCAAGC ACGACTTCCG GGTGTGGAAT 
GCTCAGCTCA TCCGCTATGC TGGCTACCAG ATGCCAGATG GCAGCATCAG AGGGGACCCT 
GCCAACGTGG AATTCACTCA GCTGTGCATC GACCTGGGCT GGAAGCCCAA GTACGGCCGC 
TTCGATGTGG TCCCCCTGGT CCTGCAGGCC AATGGCCGTG ACCCTGAGCT CTTCGAAATC 
CCACCTGACC TTGTGCTTGA GGTGGCCATG GAACATCCCA AATACGAGTG GTTTCGGGAA 
CTGGAGCTAA AGTGGTACGC CCTGCCTGCA GTGGCCAACA TGCTGCTTGA GGTGGGCGGC 
CTGGAGTTCC CAGGGTGCCC CTTCAATGGC TGGTACATGG GCACAGAGAT CGGAGTCCGG 
GACTTCTGTG ACGTCCAGCG CTACAACATC CTGGAGGAAG TGGGCAGGAG AATGGGCTTG 
GAAACGCACA AGCTGGCCTC GCTCTGGAAA GACCAGGCTG TCGTTGAGAT CAACATTGCT 
GTGCTCCATA GTTTCCAGAA GCAGAATGTG ACCATCATGG ACCACCACTC GGCTGCAGAA 
TCCTTCATGA AGTACATGCA GAATGAATAC CGGTCCCGTG GGGGCTGCCC GGCAGACTGG 
ATTTGGCTGG TCCCTCCCAT GTCTGGGAGC ATCACCCCCG TGTTTCACCA GGAGATGCTG 
AACTACGTCC TGTCCCCTTT CTACTACTAT CAGGTAGAGG CCTGGAAAAC CCATGTCTGG 
CAGGACGAGA AGCGGAGACC CAAGAGAAGA GAGATTCCAT TGAAAGTCTT GGTCAAAGCT 
GTGCTCTTTG CCTGTATGCT GATGCGCAAG ACAATGGCGT CCCGAGTCAG AGTCACCATC 
CTCTTTGCGA CAGAGACAGG AAAATCAGAG GCGCTGGCCT GGGACCTGGG GGCCTTGTTC 
AGCTGTGCCT TCAACCCCAA GGTTGTCTGC ATGGATAAGT ACAGGCTGAG CTGCCTGGAG 
GAGGAGCGGC TGCTGTTGGT GGTGACCAGT ACGTTTGGCA ATGGAGACTG CCCCGGCAAT 
GGAGAGAAAC TGAAGAAATC GCTCTTCATG CTGAAAGAGC TCAACAACAA ATTCAGGTAC 
GCTGTGTTTG GCCTCGGCTC CAGCATGTAC CCTCGGTTCT GCGCCTTTGC TCATGACATT 
GATCAGAAGC TGTCCCACCT GGGGGCCTCT CAGCTCACCC CGATGGGAGA AGGGGATGAG 
CTCAGTGGGC AGGAGGACGC CTTCCGCAGC TGGGCCGTGC AAACCTTCAA GGCAGCCTGT 
GAGATGTTTG ATGTCCGAGG CAAACAGCAC ATTCAGATCC CCAAGCTCTA CACCTCCAAT 
GTGACCTGGG ACCCGCACCA CTACAGGCTC GTGCAGGACT CACAGCCTTT GGACCTCAGC 
AAAGCCCTCA GCAGCATGCA TGCCAAGAAC GTGTTCACCA TGAGGCTCAA ATCTCGGCAG 
AATCTACAAA GTCCGACATC CAGCCGTGCC ACCATCCTGG TGGAACTCTC CTGTGAGGAT 
GGCCAAGGCC TGAACTACCT GCCGGGGGAG CACCTTGGGG TTTGCCCAGG CAACCAGCCG 
GCCCTGGTCC AAGGCATCCT GGAGCGAGTG GTGGATGGCC CCACACCCCA CCAGACAGTG 
CACCTGGAGG CCCTGGATGA GAGTGGCAGC TACTGGGTCA GTGACAAGAG GCTGCCCCCC 
TGCTCACTCA GCCAGGCCCT CACCTACTTC CTGGACATCA CAACACCCCC AACCCAGCTG 
CTGCTCCTAA AGCTGGCCCA GGTGGCCACA GAAGCGACTG AGAGACAGAG GCTGGAGGCC 
CTTTGCCAGC CCTCAGAGTA CAGCAAGTGG AAGTTCACCA ACAGCCCCAC ATTCCTGGAG 
GTGCTAGAGG AGTTCCCGTC CCTGCGGGTG TCTGCTGGCT TCCTGCTTTC CCAGCTCCCC 
ATTCTGAAGC CCAGGTTCTA CTCCATCAGC TCCTCCCGGG ATCACACGCC CACGGAGATC 
CACCTGACTG TGGCCGTGGT CACCTACCAC ACCCGAGATG GCCAGGGTCC CCTGCACCAC 
GGCGTCTGCA GCACTTGGCT CAACAGCCTG AAGCCCCAAG ACCCAGTGCC CTGCTTTGTG 
CGGAATGCCA GCGGCTTCCA CCTCCCCGAG GATCCCTCCC ATCCTTGCAT CCTCATCGGG 
CCTGGCACAG GCATCGCGCC CTTCCGCAGT TTCTGGCAGC AACGGCTCCA TGACTCCCAG 
CACAAGGGAG TGCGGGGAGG CCGCATGACC TTGGTGTTTG GGTGCCGCCG CCCAGATGAG 
GACCACATCT ACCAGGAGGA GATGCTGGAG ATGGCCCAGA AGGGGGTGCT GCATGCGGTG 
CACACAGCCT ATTCCCGCCT GCCTGGCAAG CCCAAGGTCT ATGTTCAGGA CATCCTGCGG 
CAGCAGCTGG CCAGCGAGGT GCTCCGTGTG CTCCACAAGG AGCCAGGCCA CCTCTATGTT 
TGCGGGGATG TGCGCATGGC CCGGGACGTG GCCCACACCC TGAAGCAGCT GGTGGCTGCC 
AAGCTGAAAT TGAATGAGGA GCAGGTCGAG GACTATTTCT TTCAGCTCAA GAGCCAGAAG 
CGCTATCACG AAGATATCTT TGGTGCTGTA TTTCCCTACG AGGCGAAGAA GGACAGGGTG 
GCGGTGCAGC CCAGCAACCT GGAGATGTCA GCGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NOS2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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