nos2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nos2a
Entrez Gene ID	404036
Full Name	nitric oxide synthase 2a, inducible
Synonyms	inosa,nos2,si:dkey-57m14.4
General protein information	Preferred Names nitric oxide synthase 2a, inducible Names nitric oxide synthase 2a, inducible inducible nitric oxide synthase a nitric oxide synthase 2, inducible, macrophage
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001104937.1	NP_001098407.1	nitric oxide synthase 2a, inducible
XM_005165295.3	XP_005165352.1	nitric oxide synthase 2a, inducible isoform X1
XM_005165296.3	XP_005165353.1	nitric oxide synthase 2a, inducible isoform X1
XM_005165296.4	XP_005165353.1	nitric oxide synthase 2a, inducible isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NOS2	NP_001070267.1
Canis lupus familiaris (dog)	NOS2	XP_005624903.1
Xenopus tropicalis (tropical clawed frog)	nos2	XP_002935342.2
Macaca mulatta (Rhesus monkey)	NOS2	XP_001106245.1
Rattus norvegicus (Norway rat)	Nos2	NP_036743.3
Danio rerio (zebrafish)	nos2a	NP_001098407.1
Homo sapiens (human)	NOS2	NP_000616.3
Rattus norvegicus (Norway rat)	LOC497963	XP_006247011.1
Gallus gallus (chicken)	NOS2	NP_990292.1
Pan troglodytes (chimpanzee)	NOS2	XP_001148238.1
Mus musculus (house mouse)	Nos2	NP_035057.1
Danio rerio (zebrafish)	nos2b	NP_001106973.1

Gene Ontology
Bibliography

Related articles in PubMed

Negative effects of acute cadmium on stress defense, immunity, and metal homeostasis in liver of zebrafish: The protective role of environmental zinc dpre-exposure.
Wang CC, Si LF, Guo SN, Zheng JL
Chemosphere22291-97(2019 May)

Dietary administration of Bacillus amyloliquefaciens R8 reduces hepatic oxidative stress and enhances nutrient metabolism and immunity against Aeromonas hydrophila and Streptococcus agalactiae in zebrafish (Danio rerio).
Lin YS, Saputra F, Chen YC, Hu SY
Fish & shellfish immunology86410-419(2019 Mar)

Knockout of longevity gene Sirt1 in zebrafish leads to oxidative injury, chronic inflammation, and reduced life span.
Kim DH, Jung IH, Kim DH, Park SW
PloS one14(8)e0220581(2019)

Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation.
Wheeler MA, Jaronen M, Covacu R, Zandee SEJ, Scalisi G, Rothhammer V, Tjon EC, Chao CC, Kenison JE, Blain M, Rao VTS, Hewson P, Barroso A, Gutiérrez-Vázquez C, Prat A, Antel JP, Hauser R, Quintana FJ
Cell176(3)581-596.e18(2019 01)

Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis.
Van Gennip JLM, Boswell CW, Ciruna B
Science advances4(12)eaav1781(2018 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nos2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nos2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00438	NM_001104937.1 Latest version!	Danio rerio nitric oxide synthase 2a, inducible (nos2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa10688	XM_005165295.3 Latest version!	Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa10688	XM_005165296.4 Latest version!	Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa10688	XM_005165296.3	Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nos2a ( NM_001104937.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001098407.1 Danio rerio nitric oxide synthase 2a, inducible (nos2a), mRNA.

Price & Availability↑

CloneID	ODa00438
Clone ID Related Accession (Same CDS sequence)	NM_001104937.1
Accession Version	NM_001104937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576771200000
Organism	Danio rerio(zebrafish)
Product	nitric oxide synthase 2a, inducible
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AM084880.1. On Sep 16, 2007 this sequence version replaced XM_001333956.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AM084880.1, AM749801.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA708836, SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT 
GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA 
AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC 
ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG 
TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG 
ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG 
GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC 
GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTGAAG 
TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCAATCACTG TGTTCCCTCA GAGAACAGAT 
GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG 
GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG 
CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT 
GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA 
CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG 
GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG 
TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG 
GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC 
CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC 
ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT 
TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG 
ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG 
ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG 
GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC 
CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC 
TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG 
GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC 
TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG 
CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA 
CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT 
ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG 
GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT 
GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC 
ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT 
CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG 
CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT 
TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT 
CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA 
TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG 
GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGC GGCGGATCAG 
GAGGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG 
AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC 
CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC 
TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG 
ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC 
AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA 
GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT 
TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC 
CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG 
TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA 
CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT 
CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG 
GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA 
GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001098407.1
CDS	83..3322
Translation	MGRQAQTTIIENEKQTVQSKRPVDRPIRCPFAVQLRNYKDGSTHQDSLHHKAAKNTPCNSKTCEGSIMNPKPLIRGPSKITPSPDDMLSQAEDFIDQYYKSLKIPGSTEQQARIDRIALEITLTGTYSLTTEELAFGAKQAWRNAPRCIGRIQWSNLQLFDARDCKTAEDMFHALCTHLKYATNGGNLRSAITVFPQRTDEGHDFRVWNGQLIKYAGYQMDDGSVIGDPAGVDFTQVCIQLGWKPKYGRFDVLPLVLQANGEDPEVFEIPPELILEVPIEHPEYEWFKDLDLKWYAIPAVANMLMEVGGLEFTACPFNGWYMGTEIGVRDFCDSQRYNILERVGQLMGLETRKLSSLWKDQALVAINVAVIHSYQKNKVTITDHHSASESFMQHLETEVRLRGGCPADWVWLVPPMSGSLTPVYHQELLNYILSPFFYYQTDPWLTHKWKDEKKGLRRCKVSFKGLIRAVFFSQTLIKAVLAKRVRCTVLYATETGKSLSFAKKLNTMLNRAFDSRLVCMEEYDFSELETETLLMVVTSTFGNGESPGNGVTFRKQLFSLQKVKNTIRYGVFGLGSRVYPQFCAFAHAVDDKLAELGAKRMTATGEGDELNGQDEAFSAWACTAFKDACEEFNIKKQLKAVEEQTEGWDPQRYRVQQDSCTLDPITALSALHSKTVFPMTLKRRQKLQSAQSSRSTILVELKTDGNTDALKFAPGDHVGIFPENSSELVHGIVKHLPDATPDNQSLRLETLSESSHEGKRWQTDERLPACTLVQALTYFLDVTTPPSQSLLRKLSTAADQEEDRKRLEALASDFQEYSTWKDFYRPTFLEVLEEFPSLKVPSAFILSQIPLLKPRLYSVSSSLDHHPNELHLTISVVEYQTQGGAGPMHFGTCSTWFNTIKRGHTVPCFVHRSGGFHLPEDPSTPVLLIGAGSGIAPFRSFWQQRTQDVKKTGQQISPMTLLFGCRDSETDHLYKVETLKLRLNGILGNVNTAYSRQPGKPKMYVQDVLKEQLVEKALEVLHKSAGHLYVCGGMNMAKDVAQTLKDILVNNTIMSEVEAGEYMDMLKVEKRYHEDIFGA

Target ORF information:

RefSeq Version	NM_001104937.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nitric oxide synthase 2a, inducible (nos2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104937.1

ORF Insert Sequence:

ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT 
GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA 
AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC 
ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG 
TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG 
ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG 
GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC 
GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTGAAG 
TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCAATCACTG TGTTCCCTCA GAGAACAGAT 
GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG 
GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG 
CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT 
GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA 
CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG 
GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG 
TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG 
GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC 
CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC 
ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT 
TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG 
ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG 
ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG 
GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC 
CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC 
TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG 
GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC 
TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG 
CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA 
CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT 
ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG 
GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT 
GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC 
ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT 
CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG 
CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT 
TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT 
CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA 
TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG 
GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGC GGCGGATCAG 
GAGGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG 
AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC 
CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC 
TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG 
ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC 
AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA 
GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT 
TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC 
CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG 
TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA 
CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT 
CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG 
GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA 
GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos2a ( XM_005165295.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165352.1 Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10688
Clone ID Related Accession (Same CDS sequence)	XM_005165295.3 , XM_005165296.3 , XM_005165296.4
Accession Version	XM_005165295.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nitric oxide synthase 2a, inducible isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165295.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT 
GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA 
AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC 
ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG 
TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG 
ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG 
GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC 
GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG 
TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT 
GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG 
GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG 
CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT 
GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA 
CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG 
GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG 
TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG 
GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC 
CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC 
ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT 
TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG 
ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG 
ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG 
GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC 
CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC 
TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG 
GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC 
TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG 
CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA 
CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT 
ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG 
GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT 
GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC 
ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT 
CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG 
CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT 
TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT 
CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA 
TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG 
GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG 
GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG 
AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC 
CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC 
TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG 
ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC 
AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA 
GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT 
TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC 
CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG 
TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA 
CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT 
CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG 
GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA 
GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165352.1
CDS	255..3494
Translation	MGRQAQTTIIENEKQTVQSKRPVDRPIRCPFAVQLRNYKDGSTHQDSLHHKAAKNTPCNSKTCEGSIMNPKPLIRGPSKITPSPDDMLSQAEDFIDQYYKSLKIPGSTEQQARIDRIALEITLTGTYSLTTEELAFGAKQAWRNAPRCIGRIQWSNLQLFDARDCKTAEDMFHALCTHLKYATNGGNLRSAITVFPQRTDEGHDFRVWNGQLIKYAGYQMDDGSVIGDPAGVDFTQVCIQLGWKPKYGRFDVLPLVLQANGEDPEVFEIPPELILEVPIEHPEYEWFKDLDLKWYAIPAVANMLMEVGGLEFTACPFNGWYMGTEIGVRDFCDSQRYNILERVGQLMGLETRKLSSLWKDQALVAINVAVIHSYQKNKVTITDHHSASESFMQHLETEVRLRGGCPADWVWLVPPMSGSLTPVYHQELLNYILSPFFYYQTDPWLTHKWKDEKKGLRRCKVSFKGLIRAVFFSQTLIKAVLAKRVRCTVLYATETGKSLSFAKKLNTMLNRAFDSRLVCMEEYDFSELETETLLMVVTSTFGNGESPGNGVTFRKQLFSLQKVKNTIRYGVFGLGSRVYPQFCAFAHAVDDKLAELGAKRMTATGEGDELNGQDEAFSAWACTAFKDACEEFNIKKQLKAVEEQTEGWDPQRYRVQQDSCTLDPITALSALHSKTVFPMTLKRRQKLQSAQSSRSTILVELKTDGNTDALKFAPGDHVGIFPENSSELVHGIVKHLPDATPDNQSLRLETLSESSHEGKRWQTDERLPACTLVQALTYFLDVTTPPSQSLLRKLSTVADQEEDRKRLEALASDFQEYSTWKDFYRPTFLEVLEEFPSLKVPSAFILSQIPLLKPRLYSVSSSLDHHPNELHLTISVVEYQTQGGAGPMHFGTCSTWFNTIKRGHTVPCFVHRSGGFHLPEDPSTPVLLIGAGSGIAPFRSFWQQRTQDVKKTGQQISPMTLLFGCRDSETDHLYKVETLKLRLNGILGNVNTAYSRQPGKPKMYVQDVLKEQLVEKALEVLHKSAGHLYVCGGMNMAKDVAQTLKDILVNNTIMSEVEAGEYMDMLKVEKRYHEDIFGA

Target ORF information:

RefSeq Version	XM_005165295.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165295.3

ORF Insert Sequence:

ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT 
GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA 
AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC 
ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG 
TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG 
ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG 
GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC 
GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG 
TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT 
GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG 
GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG 
CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT 
GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA 
CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG 
GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG 
TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG 
GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC 
CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC 
ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT 
TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG 
ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG 
ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG 
GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC 
CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC 
TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG 
GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC 
TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG 
CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA 
CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT 
ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG 
GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT 
GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC 
ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT 
CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG 
CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT 
TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT 
CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA 
TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG 
GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG 
GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG 
AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC 
CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC 
TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG 
ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC 
AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA 
GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT 
TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC 
CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG 
TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA 
CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT 
CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG 
GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA 
GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos2a ( XM_005165296.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165353.1 Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10688
Clone ID Related Accession (Same CDS sequence)	XM_005165295.3 , XM_005165296.3 , XM_005165296.4
Accession Version	XM_005165296.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nitric oxide synthase 2a, inducible isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165296.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT 
GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA 
AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC 
ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG 
TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG 
ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG 
GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC 
GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG 
TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT 
GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG 
GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG 
CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT 
GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA 
CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG 
GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG 
TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG 
GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC 
CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC 
ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT 
TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG 
ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG 
ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG 
GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC 
CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC 
TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG 
GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC 
TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG 
CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA 
CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT 
ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG 
GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT 
GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC 
ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT 
CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG 
CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT 
TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT 
CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA 
TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG 
GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG 
GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG 
AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC 
CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC 
TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG 
ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC 
AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA 
GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT 
TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC 
CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG 
TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA 
CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT 
CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG 
GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA 
GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165353.1
CDS	169..3408
Translation	MGRQAQTTIIENEKQTVQSKRPVDRPIRCPFAVQLRNYKDGSTHQDSLHHKAAKNTPCNSKTCEGSIMNPKPLIRGPSKITPSPDDMLSQAEDFIDQYYKSLKIPGSTEQQARIDRIALEITLTGTYSLTTEELAFGAKQAWRNAPRCIGRIQWSNLQLFDARDCKTAEDMFHALCTHLKYATNGGNLRSAITVFPQRTDEGHDFRVWNGQLIKYAGYQMDDGSVIGDPAGVDFTQVCIQLGWKPKYGRFDVLPLVLQANGEDPEVFEIPPELILEVPIEHPEYEWFKDLDLKWYAIPAVANMLMEVGGLEFTACPFNGWYMGTEIGVRDFCDSQRYNILERVGQLMGLETRKLSSLWKDQALVAINVAVIHSYQKNKVTITDHHSASESFMQHLETEVRLRGGCPADWVWLVPPMSGSLTPVYHQELLNYILSPFFYYQTDPWLTHKWKDEKKGLRRCKVSFKGLIRAVFFSQTLIKAVLAKRVRCTVLYATETGKSLSFAKKLNTMLNRAFDSRLVCMEEYDFSELETETLLMVVTSTFGNGESPGNGVTFRKQLFSLQKVKNTIRYGVFGLGSRVYPQFCAFAHAVDDKLAELGAKRMTATGEGDELNGQDEAFSAWACTAFKDACEEFNIKKQLKAVEEQTEGWDPQRYRVQQDSCTLDPITALSALHSKTVFPMTLKRRQKLQSAQSSRSTILVELKTDGNTDALKFAPGDHVGIFPENSSELVHGIVKHLPDATPDNQSLRLETLSESSHEGKRWQTDERLPACTLVQALTYFLDVTTPPSQSLLRKLSTVADQEEDRKRLEALASDFQEYSTWKDFYRPTFLEVLEEFPSLKVPSAFILSQIPLLKPRLYSVSSSLDHHPNELHLTISVVEYQTQGGAGPMHFGTCSTWFNTIKRGHTVPCFVHRSGGFHLPEDPSTPVLLIGAGSGIAPFRSFWQQRTQDVKKTGQQISPMTLLFGCRDSETDHLYKVETLKLRLNGILGNVNTAYSRQPGKPKMYVQDVLKEQLVEKALEVLHKSAGHLYVCGGMNMAKDVAQTLKDILVNNTIMSEVEAGEYMDMLKVEKRYHEDIFGA

Target ORF information:

RefSeq Version	XM_005165296.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165296.3

ORF Insert Sequence:

ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT 
GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA 
AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC 
ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG 
TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG 
ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG 
GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC 
GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG 
TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT 
GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG 
GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG 
CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT 
GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA 
CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG 
GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG 
TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG 
GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC 
CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC 
ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT 
TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG 
ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG 
ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG 
GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC 
CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC 
TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG 
GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC 
TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG 
CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA 
CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT 
ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG 
GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT 
GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC 
ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT 
CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG 
CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT 
TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT 
CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA 
TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG 
GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG 
GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG 
AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC 
CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC 
TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG 
ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC 
AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA 
GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT 
TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC 
CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG 
TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA 
CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT 
CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG 
GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA 
GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos2a ( XM_005165296.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165353.1 Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10688
Clone ID Related Accession (Same CDS sequence)	XM_005165295.3 , XM_005165296.3 , XM_005165296.4
Accession Version	XM_005165296.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nitric oxide synthase 2a, inducible isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165296.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT 
GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA 
AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC 
ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG 
TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG 
ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG 
GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC 
GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG 
TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT 
GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG 
GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG 
CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT 
GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA 
CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG 
GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG 
TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG 
GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC 
CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC 
ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT 
TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG 
ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG 
ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG 
GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC 
CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC 
TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG 
GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC 
TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG 
CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA 
CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT 
ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG 
GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT 
GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC 
ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT 
CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG 
CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT 
TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT 
CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA 
TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG 
GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG 
GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG 
AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC 
CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC 
TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG 
ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC 
AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA 
GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT 
TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC 
CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG 
TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA 
CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT 
CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG 
GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA 
GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165353.1
CDS	151..3390
Translation	MGRQAQTTIIENEKQTVQSKRPVDRPIRCPFAVQLRNYKDGSTHQDSLHHKAAKNTPCNSKTCEGSIMNPKPLIRGPSKITPSPDDMLSQAEDFIDQYYKSLKIPGSTEQQARIDRIALEITLTGTYSLTTEELAFGAKQAWRNAPRCIGRIQWSNLQLFDARDCKTAEDMFHALCTHLKYATNGGNLRSAITVFPQRTDEGHDFRVWNGQLIKYAGYQMDDGSVIGDPAGVDFTQVCIQLGWKPKYGRFDVLPLVLQANGEDPEVFEIPPELILEVPIEHPEYEWFKDLDLKWYAIPAVANMLMEVGGLEFTACPFNGWYMGTEIGVRDFCDSQRYNILERVGQLMGLETRKLSSLWKDQALVAINVAVIHSYQKNKVTITDHHSASESFMQHLETEVRLRGGCPADWVWLVPPMSGSLTPVYHQELLNYILSPFFYYQTDPWLTHKWKDEKKGLRRCKVSFKGLIRAVFFSQTLIKAVLAKRVRCTVLYATETGKSLSFAKKLNTMLNRAFDSRLVCMEEYDFSELETETLLMVVTSTFGNGESPGNGVTFRKQLFSLQKVKNTIRYGVFGLGSRVYPQFCAFAHAVDDKLAELGAKRMTATGEGDELNGQDEAFSAWACTAFKDACEEFNIKKQLKAVEEQTEGWDPQRYRVQQDSCTLDPITALSALHSKTVFPMTLKRRQKLQSAQSSRSTILVELKTDGNTDALKFAPGDHVGIFPENSSELVHGIVKHLPDATPDNQSLRLETLSESSHEGKRWQTDERLPACTLVQALTYFLDVTTPPSQSLLRKLSTVADQEEDRKRLEALASDFQEYSTWKDFYRPTFLEVLEEFPSLKVPSAFILSQIPLLKPRLYSVSSSLDHHPNELHLTISVVEYQTQGGAGPMHFGTCSTWFNTIKRGHTVPCFVHRSGGFHLPEDPSTPVLLIGAGSGIAPFRSFWQQRTQDVKKTGQQISPMTLLFGCRDSETDHLYKVETLKLRLNGILGNVNTAYSRQPGKPKMYVQDVLKEQLVEKALEVLHKSAGHLYVCGGMNMAKDVAQTLKDILVNNTIMSEVEAGEYMDMLKVEKRYHEDIFGA

Target ORF information:

RefSeq Version	XM_005165296.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nitric oxide synthase 2a, inducible (nos2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165296.4

ORF Insert Sequence:

ATGGGAAGAC AAGCACAAAC CACTATCATT GAAAATGAGA AACAGACTGT GCAATCAAAG 
CGGCCAGTCG ATCGACCAAT TCGATGTCCA TTCGCTGTTC AGTTACGCAA CTACAAGGAT 
GGCTCGACCC ACCAGGACTC ACTTCATCAC AAAGCGGCTA AGAATACGCC CTGCAATTCA 
AAAACTTGTG AGGGCTCCAT TATGAATCCC AAACCTCTGA TCCGCGGCCC ATCCAAAATC 
ACACCTTCCC CAGATGACAT GCTCAGCCAG GCAGAGGATT TCATCGACCA GTACTACAAG 
TCGCTCAAAA TCCCGGGCTC GACGGAGCAG CAAGCGCGGA TTGACAGGAT CGCGCTGGAG 
ATCACTCTGA CCGGGACCTA CTCTCTCACC ACCGAGGAAC TCGCTTTTGG GGCCAAACAG 
GCGTGGAGAA ATGCGCCAAG ATGCATTGGC AGGATCCAGT GGTCCAATTT ACAGCTTTTC 
GATGCCCGTG ATTGTAAAAC CGCTGAGGAC ATGTTTCATG CCTTGTGCAC TCATCTTAAG 
TATGCCACCA ACGGAGGAAA CTTGAGGTCT GCCATCACCG TGTTCCCTCA GAGAACAGAT 
GAAGGGCATG ATTTCCGTGT TTGGAACGGT CAGCTGATAA AGTATGCTGG CTATCAGATG 
GATGATGGTA GTGTGATCGG CGACCCAGCG GGTGTAGACT TTACACAGGT GTGTATTCAG 
CTTGGGTGGA AACCAAAGTA CGGTCGCTTT GATGTGCTTC CACTAGTCCT GCAGGCGAAT 
GGAGAAGACC CTGAAGTTTT TGAAATTCCT CCTGAACTGA TCTTGGAGGT CCCCATTGAA 
CATCCAGAGT ATGAATGGTT CAAAGATCTA GACCTGAAGT GGTACGCGAT TCCTGCAGTG 
GCCAACATGC TCATGGAAGT TGGTGGTCTG GAATTCACAG CATGTCCTTT CAACGGCTGG 
TACATGGGCA CAGAAATTGG AGTTCGGGAC TTCTGTGACA GTCAACGCTA CAACATTTTG 
GAGCGCGTTG GCCAGTTAAT GGGCTTAGAA ACACGCAAGC TCTCATCACT GTGGAAAGAC 
CAGGCACTCG TGGCTATCAA TGTTGCCGTC ATTCACAGCT ATCAGAAAAA TAAGGTCACC 
ATCACAGATC ACCATTCCGC AAGCGAGTCC TTCATGCAGC ATTTGGAGAC GGAGGTTCGT 
TTGCGTGGAG GCTGCCCAGC GGATTGGGTC TGGCTGGTGC CACCGATGTC TGGTTCCCTG 
ACCCCCGTCT ATCATCAGGA GTTGCTCAAT TACATCCTGT CCCCATTCTT CTATTACCAG 
ACTGATCCAT GGCTAACACA CAAGTGGAAA GATGAGAAGA AAGGACTTCG GAGATGCAAG 
GTCAGCTTCA AAGGACTGAT CAGAGCTGTA TTTTTCTCTC AAACACTCAT CAAAGCTGTC 
CTGGCCAAGA GAGTGCGCTG TACTGTTCTC TATGCCACAG AAACAGGGAA GTCACTGAGC 
TTTGCCAAAA AACTGAACAC CATGCTCAAC CGGGCCTTTG ACTCCAGGCT GGTATGCATG 
GAGGAATATG ACTTCAGTGA ACTTGAGACA GAGACCCTTC TGATGGTGGT CACCAGTACC 
TTTGGCAATG GAGAAAGTCC AGGAAATGGA GTGACCTTCA GGAAGCAGCT TTTTAGCTTG 
CAAAAAGTGA AAAACACCAT CAGGTACGGT GTCTTTGGCC TGGGTTCACG TGTATACCCA 
CAGTTCTGTG CCTTTGCCCA TGCTGTGGAT GACAAGCTTG CGGAGCTGGG GGCCAAACGT 
ATGACAGCCA CAGGCGAGGG TGATGAACTA AACGGGCAGG ACGAGGCTTT CTCTGCCTGG 
GCTTGCACTG CTTTTAAGGA TGCTTGTGAG GAGTTTAACA TTAAAAAGCA GCTGAAAGCT 
GTGGAGGAGC AGACGGAAGG CTGGGACCCT CAGAGATACA GAGTCCAGCA GGACAGTTGC 
ACTCTGGATC CCATTACAGC ACTGTCTGCT CTTCACTCTA AAACCGTGTT TCCTATGACT 
CTGAAGAGAA GACAGAAACT GCAAAGTGCT CAGTCAAGTC GTTCTACTAT CCTGGTGGAG 
CTGAAGACCG ATGGAAACAC AGATGCCTTG AAGTTTGCCC CAGGAGATCA TGTGGGGATT 
TTTCCAGAGA ATTCTTCAGA ATTGGTGCAT GGTATCGTGA AGCATCTCCC AGATGCCACT 
CCTGATAACC AGAGCCTTCG TCTGGAGACC CTCTCTGAGT CCAGCCATGA GGGAAAGAGA 
TGGCAGACAG ACGAACGTCT CCCTGCTTGC ACGCTCGTCC AGGCTCTAAC ATACTTCCTG 
GATGTCACGA CTCCACCTTC ACAAAGCCTG CTGCGCAAAC TCTCTACAGT GGCGGATCAG 
GAAGAGGATC GCAAACGACT TGAAGCGCTC GCATCGGACT TCCAGGAATA TTCCACATGG 
AAGGATTTCT ACAGGCCCAC CTTCTTGGAG GTTCTTGAAG AATTTCCCTC TCTGAAAGTC 
CCTTCCGCCT TCATCCTGAG TCAGATTCCT CTGCTGAAGC CCCGTCTGTA CTCCGTCAGC 
TCCTCGCTGG ATCATCATCC CAATGAACTA CACCTAACAA TCTCTGTGGT GGAGTACCAG 
ACCCAGGGTG GAGCAGGCCC AATGCATTTC GGCACTTGCA GCACGTGGTT CAACACCATC 
AAAAGAGGAC ACACAGTTCC CTGCTTTGTT CACCGCTCGG GGGGTTTCCA TCTCCCAGAA 
GACCCCAGCA CTCCTGTTCT CCTGATCGGG GCTGGCAGTG GAATTGCTCC TTTCCGCAGT 
TTCTGGCAGC AGCGCACTCA GGATGTGAAA AAGACAGGTC AGCAAATTAG CCCCATGACC 
CTACTGTTTG GCTGTCGCGA TTCAGAAACA GACCACCTGT ACAAAGTAGA AACCCTGAAG 
TTGAGACTTA ATGGTATTCT GGGAAACGTC AATACAGCTT ATTCACGCCA GCCTGGGAAA 
CCCAAGATGT ACGTCCAGGA TGTTTTGAAG GAGCAGCTTG TTGAAAAGGC TTTGGAGGTT 
CTTCACAAGA GCGCAGGTCA TCTTTATGTC TGCGGTGGAA TGAACATGGC CAAAGATGTG 
GCTCAAACTT TGAAAGATAT CCTGGTCAAC AACACAATCA TGAGCGAGGT AGAAGCTGGA 
GAATATATGG ATATGTTGAA GGTTGAGAAG AGATACCATG AAGACATTTT TGGAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Negative effects of acute cadmium on stress defense, immunity, and metal homeostasis in liver of zebrafish: The protective role of environmental zinc dpre-exposure.
Chemosphere22291-97(2019 May)
Wang CC,Si LF,Guo SN,Zheng JL

Dietary administration of Bacillus amyloliquefaciens R8 reduces hepatic oxidative stress and enhances nutrient metabolism and immunity against Aeromonas hydrophila and Streptococcus agalactiae in zebrafish (Danio rerio).
Fish & shellfish immunology86410-419(2019 Mar)
Lin YS,Saputra F,Chen YC,Hu SY

Knockout of longevity gene Sirt1 in zebrafish leads to oxidative injury, chronic inflammation, and reduced life span.
PloS one14(8)e0220581(2019)
Kim DH,Jung IH,Kim DH,Park SW

Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation.
Cell176(3)581-596.e18(2019 01)
Wheeler MA,Jaronen M,Covacu R,Zandee SEJ,Scalisi G,Rothhammer V,Tjon EC,Chao CC,Kenison JE,Blain M,Rao VTS,Hewson P,Barroso A,Gutiérrez-Vázquez C,Prat A,Antel JP,Hauser R,Quintana FJ

Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis.
Science advances4(12)eaav1781(2018 12)
Van Gennip JLM,Boswell CW,Ciruna B

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nos2a cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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