Myh4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myh4
Entrez Gene ID	17884
Full Name	myosin, heavy polypeptide 4, skeletal muscle
Synonyms	AI506973,MHC2B,MM,MYH-2B,Minimsc,Minmus,MyHC-IIb,Myhsf
General protein information	Preferred Names myosin, heavy polypeptide 4, skeletal muscle Names myosin-4 myHC-2b myosin heavy chain 2b myosin heavy chain 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B3\|11 40.59 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_010855.3	NP_034985.2	myosin-4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH4	XP_523785.2
Macaca mulatta (Rhesus monkey)	MYH4	XP_002800337.1
Canis lupus familiaris (dog)	MYH4	NP_001070262.1
Danio rerio (zebrafish)	myhz2	NP_694514.2
Danio rerio (zebrafish)	myhz1.2	NP_001154918.1
Xenopus tropicalis (tropical clawed frog)	myh3	NP_001006915.1
Danio rerio (zebrafish)	myhb	XP_001339206.5
Homo sapiens (human)	MYH4	NP_060003.2
Rattus norvegicus (Norway rat)	Myh4	NP_062198.1
Danio rerio (zebrafish)	zgc:66156	XP_002666141.3
Danio rerio (zebrafish)	ns:zf-e68	XP_001924014.2
Bos taurus (cattle)	MYH4	XP_005220494.1
Mus musculus (house mouse)	Myh4	NP_034985.2
Danio rerio (zebrafish)	myhc4	NP_001018321.1
Danio rerio (zebrafish)	myhz1.1	NP_001108561.1
Danio rerio (zebrafish)	wu:fd14a01	XP_002667379.3
Danio rerio (zebrafish)	myha	XP_001924009.1
Xenopus tropicalis (tropical clawed frog)	myh7	NP_001135564.1
Xenopus tropicalis (tropical clawed frog)	LOC100492956	XP_002937039.1
Xenopus tropicalis (tropical clawed frog)	LOC100493121	XP_004919657.1

Gene Ontology
Bibliography

Related articles in PubMed

CAP2 deficiency delays myofibril actin cytoskeleton differentiation and disturbs skeletal muscle architecture and function.
Kepser LJ, Damar F, De Cicco T, Chaponnier C, Prószyński TJ, Pagenstecher A, Rust MB
Proceedings of the National Academy of Sciences of the United States of America116(17)8397-8402(2019 Apr)

Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections.
McGlinn E, Holzman MA, Mansfield JH
Methods in molecular biology (Clifton, N.J.)1920183-218(2019)

The Trithorax protein Ash1L promotes myoblast fusion by activating Cdon expression.
Castiglioni I, Caccia R, Garcia-Manteiga JM, Ferri G, Caretti G, Molineris I, Nishioka K, Gabellini D
Nature communications9(1)5026(2018 11)

Skeletal Muscle-Specific Methyltransferase METTL21C Trimethylates p97 and Regulates Autophagy-Associated Protein Breakdown.
Wiederstein JL, Nolte H, Günther S, Piller T, Baraldo M, Kostin S, Bloch W, Schindler N, Sandri M, Blaauw B, Braun T, Hölper S, Krüger M
Cell reports23(5)1342-1356(2018 05)

ANKK1 is found in myogenic precursors and muscle fibers subtypes with glycolytic metabolism.
Rubio-Solsona E, Martí S, Vílchez JJ, Palau F, Hoenicka J
PloS one13(5)e0197254(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Myh4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myh4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19987	NM_010855.3 Latest version!	Mus musculus myosin, heavy polypeptide 4, skeletal muscle (Myh4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myh4 ( NM_010855.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034985.2 Mus musculus myosin, heavy polypeptide 4, skeletal muscle (Myh4), mRNA.

Price & Availability↑

CloneID	OMu19987
Clone ID Related Accession (Same CDS sequence)	NM_010855.3
Accession Version	NM_010855.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571587200000
Organism	Mus musculus(house mouse)
Product	myosin-4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK138608.1 and AL596129.27. On Jan 30, 2013 this sequence version replaced NM_010855.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC141362.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCCG ACGCTGAGAT GGCCGTTTTC GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC CCAGAACAAG CCTTTTGATG CCAAGTCATC GGTGTTTGTG 
GTGGATGCTA AGGAGTCCTA TGTGAAAGCA ACAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGGCGG AGCTACGGTC ACCGTCAAGG ATGATCAAGT CTTCTCCATG 
AACCCTCCCA AGTACGACAA GATTGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTTTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC TGAGGTGGTG 
GCAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAT 
AACGCCTACC AGTTCATGCT GACGGATCGT GAGAATCAGT CCATCCTGAT TACTGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG ACAAGAAGAA GGAGGAGGCG ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAAATTA TCAGTGCCAA CCCCCTGCTG GAGGCCTTCG GCAACGCCAA GACTGTGAGG 
AACGACAACT CTTCTCGATT TGGTAAATTC ATCAGAATCC ATTTCGGTGC CACAGGCAAA 
CTGGCCTCTG CAGATATTGA AACCTATCTG CTAGAGAAGT CCAGAGTCAC CTTCCAGCTC 
AAGGCTGAAA GGAGCTACCA CATCTTCTAC CAAATCATGT CCAATAAGAA ACCAGAGCTT 
ATTGAGATGC TTCTGATCAC CACCAACCCA TATGACTTTG CTTACGTCAG TCAAGGTGAA 
ATCACAGTGC CCAGCATTGA TGACCAGGAA GAGCTGATGG CCACAGACAC TGCTGTTGAT 
ATCCTGGGAT TCAGTGCAGA TGAAAAGGTG GCCATTTACA AGCTCACAGG CGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAAAGGGAAG AGCAGGCTGA GCCAGACGGC 
ACCGAAGTTG CTGACAAGGC TGCCTATCTG ACAAGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACGGTG 
CAGCAGGTGT ACAACTCCGT GGGTGCCCTG GCCAAGTCCA TGTATGAGAA GATGTTCCTA 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCCGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGACTTCA ACACCTTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATTGAT TGGGAGTTCA TTGACTTCGG GATGGACCTG 
GCGGCCTGCA TCGAGCTCAT CGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA GCAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAGCCCAAA CCCGCCAAAG GCAAGGCTGA GGCTCACTTC 
TCCCTGGTGC ACTATGCGGG CACCGTGGAC TACAACATTA TTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC CGTGGTGGGG CTGTACCAGA AATCCGGGTT GAAGACTCTG 
GCTTTCCTAT TTTCTGGGGG ACAAGCTGCG GAAGCAGAGG GCGGCGGTGG AAAGAAAGGT 
GGCAAGAAGA AGGGTTCTTC TTTCCAGACC GTGTCAGCTC TCTTCAGGGA GAATTTAAAT 
AAGCTGATGA CCAACTTGAA GAGCACCCAC CCCCACTTTG TCAGATGCCT CATTCCCAAT 
GAAACTAAGA CTCCTGGTGC CATGGAGCAT GAACTGGTCT TGCACCAGCT CAGGTGTAAC 
GGGGTGCTGG AAGGCATCCG CATCTGTAGG AAGGGGTTCC CCAGCAGGAT CCTCTATGCA 
GACTTCAAGC AGAGATACAA AGTCCTGAAT GCAAGTGCCA TCCCTGAGGG TCAGTTCATT 
GATAGCAAGA AGGCCTCTGA GAAACTTCTG GGTTCCATTG ACATTGACCA CACTCAGTAC 
AAATTCGGTC ACACTAAGGT TTTCTTTAAA GCCGGCCTGT TGGGAACTCT AGAGGAGATG 
CGAGATGAAA AGCTGGCTCA GCTCATCACC CGCACTCAAG CCGTGTGCAG AGGGTACCTG 
ATGAGGGTGG AGTTCAAAAA GATGATGGAG AGGAGAGAGT CCATCTTCTG CATTCAGTAC 
AACGTCCGCG CCTTCATGAA TGTGAAACAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAACCTCTGC TGAAGAGCGC GGAGACCGAG AAGGAGATGG CCAACATGAA GGAAGATTTT 
GAGAAGGCCA AGGAGGATCT GGCTAAGTCA GAGGCAAAAA GGAAGGAACT AGAAGAAAAG 
ATGGTAGCTT TGATGCAGGA GAAAAATGAC CTGCAGCTCC AGGTTCAAGC TGAAGCAGAT 
GGCTTGGCTG ATGCTGAGGA AAGGTGCGAC CAGCTGATCA AAACCAAAAT CCAGCTGGAG 
GCCAAAATCA AAGAGCTGAC CGAGAGGGCC GAGGACGAGG AGGAGATCAA CGCGGAGCTC 
ACGGCCAAGA AGAGGAAGCT GGAGGACGAG TGCTCAGAGC TGAAGAAGGA CATTGACGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAGCACG CCACGGAGAA CAAGGTGAAA 
AACCTGACAG AAGAGATGGC AGGCCTGGAT GAAAATATCG CCAAGCTGAC CAAGGAGAAG 
AAGGCCCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGGCAGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC CAAAACCAAG CTGGAACAGC AAGTGGATGA CCTTGAAGGA 
TCACTGGAAC AGGAAAAGAA GCTGAGGATG GACTTAGAAA GAGCCAAGAG GAAACTGGAG 
GGGGACCTGA AACTGGCCCA AGAGTCCACA ATGGACATAG AAAACGATAA ACAGCAGCTC 
GATGAGAAAC TCAAAAAGAA AGAGTTTGAA ATGAGCAATC TGCAGAGCAA GATTGAGGAC 
GAGCAGGCCC TCGGCATGCA GCTGCAGAAG AAGATCAAGG AGTTGCAGGC CCGCATCGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCAGGG CTAAAGCAGA GAAGCAGCGC 
TCTGACCTCT CCCGGGAACT GGAGGAGATC AGCGAGAGGC TGGAAGAAGC TGGCGGAGCC 
ACTTCAGCCC AGATCGAGAT GAACAAGAAG AGAGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAGG AGGCCACGCT GCAGCATGAA GCCACAGCAG CCGCTCTTCG GAAGAAGCAC 
GCGGACAGCG TGGCTGAGCT CGGGGAGCAG ATCGACAACC TGCAGCGGGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGCGA GCTGAAGATG GAGATCGATG ATCTCGCTAG TAACATGGAG 
ACAGTCTCCA AAGCCAAGGG GAACCTTGAG AAGATGTGCC GCACCCTGGA GGACCAACTG 
AGTGAAGTGA AGACCAAGGA GGAGGAGCAG CAGCGCCTGA TCAATGAACT GTCAACCCAG 
AAGGCACGCT TGCACACAGA GTCAGGCGAG TTTTCCCGAC AGCTGGATGA AAAAGACGCT 
ATGGTTTCTC AGCTTTCCCG AGGCAAACAA GCATTTACAC AACAGATTGA GGAACTAAAG 
AGGCAGCTAG AGGAAGAGTC CAAGGCCAAG AACGCACTGG CCCACGCCCT GCAGTCAGCT 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAAGAGG AGCAGGAAGC CAAGGCTGAG 
CTGCAGAGGG CCATGTCCAA GGCCAACAGC GAGGTAGCCC AGTGGAGGAC GAAATATGAG 
ACGGATGCCA TCCAGCGCAC AGAGGAGCTG GAAGAGGCCA AGAAGAAGCT GGCTCAGCGT 
CTGCAGGATG CTGAGGAACA TGTAGAAGCT GTGAATTCCA AATGCGCTTC TCTTGAAAAG 
ACGAAGCAGC GGCTTCAGAA TGAAGTGGAG GACCTCATGA TTGACGTGGA GAGGTCTAAC 
GCTGCCTGCG CTGCGCTTGA TAAGAAGCAG AGGAACTTTG ACAAGGTCCT GGCAGAATGG 
AAACAGAAGT ACGAGGAAAC TCAGGCTGAG CTTGAAGCCT CCCAGAAGGA GTCCCGCTCT 
CTGAGCACTG AGCTGTTCAA AGTGAAGAAT GCCTATGAGG AGTCTCTGGA TCAACTTGAG 
ACGTTAAAGA GAGAGAACAA GAACCTGCAG CAGGAGATCT CTGACCTGAC CGAGCAGATT 
GCTGAGGGAG GGAAGCACAT CCATGAGCTG GAGAAAATCA AGAAGCAAAT TGATCAGGAA 
AAGAGTGAAT TACAGGCTTC TCTGGAGGAA GCGGAGGCAT CCCTTGAACA TGAAGAAGGT 
AAAATCCTAC GCATCCAGCT GGAGTTGAAC CAGGTGAAAT CTGAGATTGA CCGCAAAATT 
GCTGAAAAAG ATGAAGAGAT AGATCAGCTG AAGAGAAACC ACCTCAGAGT TGTGGAGTCC 
ATGCAGAGCA CGCTGGACGC CGAGATCCGC AGCAGGAATG ATGCTCTGAG GATTAAGAAG 
AAGATGGAGG GAGACCTCAA TGAGATGGAG ATCCAGCTGA ACCATGCCAA CCGCCAGGCT 
GCGGAGGCAA TCAGGAACCT TCGGAACACC CAGGGAATGC TGAAGGACAC ACAGCTGCAC 
CTGGACGATG CTCTCAGAGG CCAGGACGAC CTGAAAGAGC AGCTGGCCAT GGTTGAGCGC 
AGAGCCAACC TGATGCAGGC TGAGATCGAG GAGCTCAGGG CATCCCTGGA ACAGACAGAG 
AGGAGCAGGA GAGTGGCCGA GCAAGAGCTA CTGGATGCCA GTGAGCGCGT GCAGCTCCTC 
CACACCCAGA ACACCAGCCT CATCAACACC AAGAAGAAGC TGGAGACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAAGA CATCGTCCAG GAAGCCCGCA ACGCAGAAGA GAAGGCCAAG 
AAAGCCATCA CTGATGCCGC CATGATGGCG GAGGAGCTGA AGAAGGAGCA GGACACCAGC 
GCCCACCTGG AGCGGATGAA GAAGAACATG GAGCAGACCG TGAAGGACCT GCAGCACCGT 
CTGGACGAGG CTGAGCAGCT GGCGCTGAAG GGCGGCAAGA AGCAGATCCA GAAACTGGAG 
GCTAGGGTGA GGGAGCTTGA AAACGAGGTG GAAAATGAAC AGAAGCGCAA CATCGAAGCC 
GTCAAGGGTC TTCGTAAGCA CGAGCGCAGA GTGAAGGAAC TCACCTACCA GACCGAGGAG 
GACCGCAAGA ACGTGCTGAG GCTGCAGGAC TTGGTGGACA AACTACAGAC TAAAGTGAAA 
GCCTACAAGA GACAGGCTGA GGAGGCTGAG GAACAATCCA ATGTCAACCT GGCCAAGTTC 
CGTAAGATCC AGCACGAGCT GGAGGAAGCC GAGGAGCGGG CTGACATCGC GGAGTCCCAG 
GTCAACAAGC TGCGGGTGAA GAGCCGAGAG GTTCACACTA AAGTCATAAG CGAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034985.2
CDS	111..5930
Translation	MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESYVKATVQSREGGKVTAKTEGGATVTVKDDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDFAYVSQGEITVPSIDDQEELMATDTAVDILGFSADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALAKSMYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNTLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIDWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNIIGWLDKNKDPLNETVVGLYQKSGLKTLAFLFSGGQAAEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLKSTHPHFVRCLIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQAVCRGYLMRVEFKKMMERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEDFEKAKEDLAKSEAKRKELEEKMVALMQEKNDLQLQVQAEADGLADAEERCDQLIKTKIQLEAKIKELTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDENIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMETVSKAKGNLEKMCRTLEDQLSEVKTKEEEQQRLINELSTQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEESKAKNALAHALQSARHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACAALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKIKKQIDQEKSELQASLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRVVESMQSTLDAEIRSRNDALRIKKKMEGDLNEMEIQLNHANRQAAEAIRNLRNTQGMLKDTQLHLDDALRGQDDLKEQLAMVERRANLMQAEIEELRASLEQTERSRRVAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELENEVENEQKRNIEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	NM_010855.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 4, skeletal muscle (Myh4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010855.3

ORF Insert Sequence:

ATGAGTTCCG ACGCTGAGAT GGCCGTTTTC GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC CCAGAACAAG CCTTTTGATG CCAAGTCATC GGTGTTTGTG 
GTGGATGCTA AGGAGTCCTA TGTGAAAGCA ACAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGGCGG AGCTACGGTC ACCGTCAAGG ATGATCAAGT CTTCTCCATG 
AACCCTCCCA AGTACGACAA GATTGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTTTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC TGAGGTGGTG 
GCAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAT 
AACGCCTACC AGTTCATGCT GACGGATCGT GAGAATCAGT CCATCCTGAT TACTGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG ACAAGAAGAA GGAGGAGGCG ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAAATTA TCAGTGCCAA CCCCCTGCTG GAGGCCTTCG GCAACGCCAA GACTGTGAGG 
AACGACAACT CTTCTCGATT TGGTAAATTC ATCAGAATCC ATTTCGGTGC CACAGGCAAA 
CTGGCCTCTG CAGATATTGA AACCTATCTG CTAGAGAAGT CCAGAGTCAC CTTCCAGCTC 
AAGGCTGAAA GGAGCTACCA CATCTTCTAC CAAATCATGT CCAATAAGAA ACCAGAGCTT 
ATTGAGATGC TTCTGATCAC CACCAACCCA TATGACTTTG CTTACGTCAG TCAAGGTGAA 
ATCACAGTGC CCAGCATTGA TGACCAGGAA GAGCTGATGG CCACAGACAC TGCTGTTGAT 
ATCCTGGGAT TCAGTGCAGA TGAAAAGGTG GCCATTTACA AGCTCACAGG CGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAAAGGGAAG AGCAGGCTGA GCCAGACGGC 
ACCGAAGTTG CTGACAAGGC TGCCTATCTG ACAAGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACGGTG 
CAGCAGGTGT ACAACTCCGT GGGTGCCCTG GCCAAGTCCA TGTATGAGAA GATGTTCCTA 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCCGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGACTTCA ACACCTTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATTGAT TGGGAGTTCA TTGACTTCGG GATGGACCTG 
GCGGCCTGCA TCGAGCTCAT CGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA GCAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAGCCCAAA CCCGCCAAAG GCAAGGCTGA GGCTCACTTC 
TCCCTGGTGC ACTATGCGGG CACCGTGGAC TACAACATTA TTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC CGTGGTGGGG CTGTACCAGA AATCCGGGTT GAAGACTCTG 
GCTTTCCTAT TTTCTGGGGG ACAAGCTGCG GAAGCAGAGG GCGGCGGTGG AAAGAAAGGT 
GGCAAGAAGA AGGGTTCTTC TTTCCAGACC GTGTCAGCTC TCTTCAGGGA GAATTTAAAT 
AAGCTGATGA CCAACTTGAA GAGCACCCAC CCCCACTTTG TCAGATGCCT CATTCCCAAT 
GAAACTAAGA CTCCTGGTGC CATGGAGCAT GAACTGGTCT TGCACCAGCT CAGGTGTAAC 
GGGGTGCTGG AAGGCATCCG CATCTGTAGG AAGGGGTTCC CCAGCAGGAT CCTCTATGCA 
GACTTCAAGC AGAGATACAA AGTCCTGAAT GCAAGTGCCA TCCCTGAGGG TCAGTTCATT 
GATAGCAAGA AGGCCTCTGA GAAACTTCTG GGTTCCATTG ACATTGACCA CACTCAGTAC 
AAATTCGGTC ACACTAAGGT TTTCTTTAAA GCCGGCCTGT TGGGAACTCT AGAGGAGATG 
CGAGATGAAA AGCTGGCTCA GCTCATCACC CGCACTCAAG CCGTGTGCAG AGGGTACCTG 
ATGAGGGTGG AGTTCAAAAA GATGATGGAG AGGAGAGAGT CCATCTTCTG CATTCAGTAC 
AACGTCCGCG CCTTCATGAA TGTGAAACAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAACCTCTGC TGAAGAGCGC GGAGACCGAG AAGGAGATGG CCAACATGAA GGAAGATTTT 
GAGAAGGCCA AGGAGGATCT GGCTAAGTCA GAGGCAAAAA GGAAGGAACT AGAAGAAAAG 
ATGGTAGCTT TGATGCAGGA GAAAAATGAC CTGCAGCTCC AGGTTCAAGC TGAAGCAGAT 
GGCTTGGCTG ATGCTGAGGA AAGGTGCGAC CAGCTGATCA AAACCAAAAT CCAGCTGGAG 
GCCAAAATCA AAGAGCTGAC CGAGAGGGCC GAGGACGAGG AGGAGATCAA CGCGGAGCTC 
ACGGCCAAGA AGAGGAAGCT GGAGGACGAG TGCTCAGAGC TGAAGAAGGA CATTGACGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAGCACG CCACGGAGAA CAAGGTGAAA 
AACCTGACAG AAGAGATGGC AGGCCTGGAT GAAAATATCG CCAAGCTGAC CAAGGAGAAG 
AAGGCCCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGGCAGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC CAAAACCAAG CTGGAACAGC AAGTGGATGA CCTTGAAGGA 
TCACTGGAAC AGGAAAAGAA GCTGAGGATG GACTTAGAAA GAGCCAAGAG GAAACTGGAG 
GGGGACCTGA AACTGGCCCA AGAGTCCACA ATGGACATAG AAAACGATAA ACAGCAGCTC 
GATGAGAAAC TCAAAAAGAA AGAGTTTGAA ATGAGCAATC TGCAGAGCAA GATTGAGGAC 
GAGCAGGCCC TCGGCATGCA GCTGCAGAAG AAGATCAAGG AGTTGCAGGC CCGCATCGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCAGGG CTAAAGCAGA GAAGCAGCGC 
TCTGACCTCT CCCGGGAACT GGAGGAGATC AGCGAGAGGC TGGAAGAAGC TGGCGGAGCC 
ACTTCAGCCC AGATCGAGAT GAACAAGAAG AGAGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAGG AGGCCACGCT GCAGCATGAA GCCACAGCAG CCGCTCTTCG GAAGAAGCAC 
GCGGACAGCG TGGCTGAGCT CGGGGAGCAG ATCGACAACC TGCAGCGGGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGCGA GCTGAAGATG GAGATCGATG ATCTCGCTAG TAACATGGAG 
ACAGTCTCCA AAGCCAAGGG GAACCTTGAG AAGATGTGCC GCACCCTGGA GGACCAACTG 
AGTGAAGTGA AGACCAAGGA GGAGGAGCAG CAGCGCCTGA TCAATGAACT GTCAACCCAG 
AAGGCACGCT TGCACACAGA GTCAGGCGAG TTTTCCCGAC AGCTGGATGA AAAAGACGCT 
ATGGTTTCTC AGCTTTCCCG AGGCAAACAA GCATTTACAC AACAGATTGA GGAACTAAAG 
AGGCAGCTAG AGGAAGAGTC CAAGGCCAAG AACGCACTGG CCCACGCCCT GCAGTCAGCT 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAAGAGG AGCAGGAAGC CAAGGCTGAG 
CTGCAGAGGG CCATGTCCAA GGCCAACAGC GAGGTAGCCC AGTGGAGGAC GAAATATGAG 
ACGGATGCCA TCCAGCGCAC AGAGGAGCTG GAAGAGGCCA AGAAGAAGCT GGCTCAGCGT 
CTGCAGGATG CTGAGGAACA TGTAGAAGCT GTGAATTCCA AATGCGCTTC TCTTGAAAAG 
ACGAAGCAGC GGCTTCAGAA TGAAGTGGAG GACCTCATGA TTGACGTGGA GAGGTCTAAC 
GCTGCCTGCG CTGCGCTTGA TAAGAAGCAG AGGAACTTTG ACAAGGTCCT GGCAGAATGG 
AAACAGAAGT ACGAGGAAAC TCAGGCTGAG CTTGAAGCCT CCCAGAAGGA GTCCCGCTCT 
CTGAGCACTG AGCTGTTCAA AGTGAAGAAT GCCTATGAGG AGTCTCTGGA TCAACTTGAG 
ACGTTAAAGA GAGAGAACAA GAACCTGCAG CAGGAGATCT CTGACCTGAC CGAGCAGATT 
GCTGAGGGAG GGAAGCACAT CCATGAGCTG GAGAAAATCA AGAAGCAAAT TGATCAGGAA 
AAGAGTGAAT TACAGGCTTC TCTGGAGGAA GCGGAGGCAT CCCTTGAACA TGAAGAAGGT 
AAAATCCTAC GCATCCAGCT GGAGTTGAAC CAGGTGAAAT CTGAGATTGA CCGCAAAATT 
GCTGAAAAAG ATGAAGAGAT AGATCAGCTG AAGAGAAACC ACCTCAGAGT TGTGGAGTCC 
ATGCAGAGCA CGCTGGACGC CGAGATCCGC AGCAGGAATG ATGCTCTGAG GATTAAGAAG 
AAGATGGAGG GAGACCTCAA TGAGATGGAG ATCCAGCTGA ACCATGCCAA CCGCCAGGCT 
GCGGAGGCAA TCAGGAACCT TCGGAACACC CAGGGAATGC TGAAGGACAC ACAGCTGCAC 
CTGGACGATG CTCTCAGAGG CCAGGACGAC CTGAAAGAGC AGCTGGCCAT GGTTGAGCGC 
AGAGCCAACC TGATGCAGGC TGAGATCGAG GAGCTCAGGG CATCCCTGGA ACAGACAGAG 
AGGAGCAGGA GAGTGGCCGA GCAAGAGCTA CTGGATGCCA GTGAGCGCGT GCAGCTCCTC 
CACACCCAGA ACACCAGCCT CATCAACACC AAGAAGAAGC TGGAGACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAAGA CATCGTCCAG GAAGCCCGCA ACGCAGAAGA GAAGGCCAAG 
AAAGCCATCA CTGATGCCGC CATGATGGCG GAGGAGCTGA AGAAGGAGCA GGACACCAGC 
GCCCACCTGG AGCGGATGAA GAAGAACATG GAGCAGACCG TGAAGGACCT GCAGCACCGT 
CTGGACGAGG CTGAGCAGCT GGCGCTGAAG GGCGGCAAGA AGCAGATCCA GAAACTGGAG 
GCTAGGGTGA GGGAGCTTGA AAACGAGGTG GAAAATGAAC AGAAGCGCAA CATCGAAGCC 
GTCAAGGGTC TTCGTAAGCA CGAGCGCAGA GTGAAGGAAC TCACCTACCA GACCGAGGAG 
GACCGCAAGA ACGTGCTGAG GCTGCAGGAC TTGGTGGACA AACTACAGAC TAAAGTGAAA 
GCCTACAAGA GACAGGCTGA GGAGGCTGAG GAACAATCCA ATGTCAACCT GGCCAAGTTC 
CGTAAGATCC AGCACGAGCT GGAGGAAGCC GAGGAGCGGG CTGACATCGC GGAGTCCCAG 
GTCAACAAGC TGCGGGTGAA GAGCCGAGAG GTTCACACTA AAGTCATAAG CGAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CAP2 deficiency delays myofibril actin cytoskeleton differentiation and disturbs skeletal muscle architecture and function.
Proceedings of the National Academy of Sciences of the United States of America116(17)8397-8402(2019 Apr)
Kepser LJ,Damar F,De Cicco T,Chaponnier C,Prószyński TJ,Pagenstecher A,Rust MB

Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections.
Methods in molecular biology (Clifton, N.J.)1920183-218(2019)
McGlinn E,Holzman MA,Mansfield JH

The Trithorax protein Ash1L promotes myoblast fusion by activating Cdon expression.
Nature communications9(1)5026(2018 11)
Castiglioni I,Caccia R,Garcia-Manteiga JM,Ferri G,Caretti G,Molineris I,Nishioka K,Gabellini D

Skeletal Muscle-Specific Methyltransferase METTL21C Trimethylates p97 and Regulates Autophagy-Associated Protein Breakdown.
Cell reports23(5)1342-1356(2018 05)
Wiederstein JL,Nolte H,Günther S,Piller T,Baraldo M,Kostin S,Bloch W,Schindler N,Sandri M,Blaauw B,Braun T,Hölper S,Krüger M

ANKK1 is found in myogenic precursors and muscle fibers subtypes with glycolytic metabolism.
PloS one13(5)e0197254(2018)
Rubio-Solsona E,Martí S,Vílchez JJ,Palau F,Hoenicka J

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Myh4 cDNA ORF clone, Mus musculus(house mouse)

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