MYH4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH4
Entrez Gene ID	4622
Full Name	myosin heavy chain 4
Synonyms	MYH2B,MyHC-2B,MyHC-IIb
General protein information	Preferred Names myosin heavy chain 4 Names myosin-4 myosin heavy chain 2b myosin heavy chain IIb myosin heavy chain, skeletal muscle, fetal myosin, heavy chain 4, skeletal muscle myosin, heavy polypeptide 4, skeletal muscle
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Disorder MIM:	160742

mRNA and Protein(s)

mRNA	Protein	Name
NM_017533.2	NP_060003.2	myosin-4
XM_017024676.1	XP_016880165.1	myosin-4 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH4	XP_523785.2
Macaca mulatta (Rhesus monkey)	MYH4	XP_002800337.1
Canis lupus familiaris (dog)	MYH4	NP_001070262.1
Danio rerio (zebrafish)	myhz2	NP_694514.2
Danio rerio (zebrafish)	myhz1.2	NP_001154918.1
Xenopus tropicalis (tropical clawed frog)	myh3	NP_001006915.1
Danio rerio (zebrafish)	myhb	XP_001339206.5
Homo sapiens (human)	MYH4	NP_060003.2
Rattus norvegicus (Norway rat)	Myh4	NP_062198.1
Danio rerio (zebrafish)	zgc:66156	XP_002666141.3
Danio rerio (zebrafish)	ns:zf-e68	XP_001924014.2
Bos taurus (cattle)	MYH4	XP_005220494.1
Mus musculus (house mouse)	Myh4	NP_034985.2
Danio rerio (zebrafish)	myhc4	NP_001018321.1
Danio rerio (zebrafish)	myhz1.1	NP_001108561.1
Danio rerio (zebrafish)	wu:fd14a01	XP_002667379.3
Danio rerio (zebrafish)	myha	XP_001924009.1
Xenopus tropicalis (tropical clawed frog)	myh7	NP_001135564.1
Xenopus tropicalis (tropical clawed frog)	LOC100492956	XP_002937039.1
Xenopus tropicalis (tropical clawed frog)	LOC100493121	XP_004919657.1

Gene Ontology
Bibliography

Related articles in PubMed

Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation.
Qiu X, Chen D, Li M, Gao Y, Liu F, Zheng H, Chen S
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery272(10)2915-23(2015 Oct)

Myosin heavy chain-2b transcripts and isoform are expressed in human laryngeal muscles.
Smerdu V, Cvetko E
Cells, tissues, organs198(1)75-86(2013)

Temporal spatial expression and function of non-muscle myosin II isoforms IIA and IIB in scar remodeling.
Bond JE, Ho TQ, Selim MA, Hunter CL, Bowers EV, Levinson H
Laboratory investigation; a journal of technical methods and pathology91(4)499-508(2011 Apr)

Mts1 regulates the assembly of nonmuscle myosin-IIA.
Li ZH, Spektor A, Varlamova O, Bresnick AR
Biochemistry42(48)14258-66(2003 Dec)

Abundant expression of myosin heavy-chain IIB RNA in a subset of human masseter muscle fibres.
Horton MJ, Brandon CA, Morris TJ, Braun TW, Yaw KM, Sciote JJ
Archives of oral biology46(11)1039-50(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYH4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu02368	NM_017533.2 Latest version!	Homo sapiens myosin heavy chain 4 (MYH4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$923.30 $1319.00
OHu02368	XM_017024676.1 Latest version!	Homo sapiens myosin heavy chain 4 (MYH4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$923.30 $1319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH4 ( NM_017533.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060003.2 Homo sapiens myosin heavy chain 4 (MYH4), mRNA.

Price & Availability↑

CloneID	OHu02368
Clone ID Related Accession (Same CDS sequence)	NM_017533.2 , XM_017024676.1
Accession Version	NM_017533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Homo sapiens(human)
Product	myosin-4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF111783.2 and AC005323.1. This sequence is a reference standard in the RefSeqGene project. On Jul 21, 2006 this sequence version replaced NM_017533.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF111783.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000255381.2/ ENSP00000255381.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGAGTTCTG ACTCTGAGAT GGCCATTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAAGGAGC GAATTGAAGC TCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTGGACCCTA AGGAGTCCTA CGTGAAAGCA ATAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGCTGG AGCTACTGTA ACTGTGAAAG AAGACCAAGT CTTCTCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCATGAGCCT 
GCTGTGCTGT ATAACCTCAA AGAGCGTTAC GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC TGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ATATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT AACTGATCGT GAAAACCAGT CAATCTTGAT TACTGGAGAA 
TCTGGTGCAG GGAAGACTGT GAACACGAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGAG AGAAGAAAAA AGAGGAACCT GCCTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAAATCA TCAGTGCTAA CCCCCTACTG GAAGCCTTCG GCAATGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ATTTTGGTGC CACAGGCAAA 
CTGGCTTCTG CAGATATTGA AACATATCTG CTAGAGAAGT CCCGAGTTAC TTTTCAGCTA 
AAGGCTGAAA GAAGCTACCA CATATTTTAT CAAATCCTGT CCAATAAGAA ACCAGAGCTC 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTTCG CATTTGTCAG CCAAGGGGAA 
ATTACTGTGC CCAGCATTGA TGACCAGGAA GAGCTGATGG CCACAGATAG TGCTGTGGAC 
ATCCTGGGTT TCACTGCTGA TGAAAAGGTG GCCATTTACA AGCTCACTGG AGCCGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAAAGGGAAG AGCAGGCAGA GCCAGATGGC 
ACGGAAGTTG CTGACAAAGC TGCTTATCTG ACAAGTCTGA ACTCTGCTGA CCTGCTCAAA 
TCTCTCTGCT ATCCCAGAGT CAAGGTCGGC AATGAGTTCG TAACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAATGCAGT GGGTGCTCTG GCCAAAGCCA TCTACGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAAGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT AGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAACATCTT 
GGAAAATCCA ACAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGCCTGA GGCTCACTTC 
TCACTGGTGC ACTATGCCGG CACCGTGGAC TACAACATCG CCGGCTGGCT GGACAAAAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTG 
GCTTTCCTCT TCTCTGGGGC ACAAACTGCT GAAGCAGAGG GTGGTGGTGG AAAGAAAGGT 
GGCAAAAAGA AGGGTTCTTC TTTCCAGACA GTGTCAGCTC TTTTCAGGGA GAATTTGAAT 
AAGCTGATGA CCAACTTGAG GAGCACTCAC CCCCACTTTG TGCGGTGCAT CATCCCCAAT 
GAAACTAAAA CTCCTGGTGC CATGGAGCAT GAGCTTGTCC TGCATCAGCT GAGGTGTAAC 
GGTGTGCTGG AAGGCATCCG CATCTGCAGG AAAGGCTTCC CAAGCAGAAT CCTTTATGCA 
GACTTCAAAC AGAGATACAA GGTTCTAAAT GCGAGTGCTA TCCCAGAGGG TCAGTTCATT 
GACAGCAAGA AGGCTTCTGA GAAACTTCTA GGGTCTATTG AAATTGACCA CACCCAGTAC 
AAATTCGGTC ATACCAAGGT TTTCTTCAAA GCTGGCCTGC TGGGAACTCT AGAGGAAATG 
CGAGATGAAA AGCTAGCTCA ACTCATCACG CGCACTCAAG CCATATGCAG AGGGTTCCTG 
ATGAGAGTGG AGTTCAGAAA GATGATGGAG AGGAGAGAGT CCATCTTCTG CATTCAGTAC 
AACATCCGTG CTTTCATGAA TGTGAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAGCCCCTCC TCAAGAGTGC AGAGACAGAG AAGGAGATGG CCAACATGAA GGAAGAATTT 
GAGAAAACCA AAGAAGAGCT GGCTAAGACA GAGGCAAAAA GGAAAGAACT AGAAGAAAAG 
ATGGTGACGC TAATGCAAGA GAAAAATGAC TTACAACTCC AAGTTCAAGC TGAAGCAGAT 
GCCTTGGCTG ATGCAGAGGA AAGATGTGAT CAGTTGATTA AAACCAAAAT CCAACTTGAG 
GCCAAAATCA AAGAGGTAAC TGAAAGAGCT GAGGATGAGG AAGAGATCAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAGC TCAAGAAAGA CATTGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAACATG CCACAGAGAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC AGGTCTGGAT GAAACCATTG CTAAGCTGAC CAAGGAGAAG 
AAGGCTCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGATGGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC TAAAACCAAG CTAGAACAGC AAGTGGACGA TCTTGAAGGA 
TCTCTGGAAC AAGAAAAGAA ACTTTGCATG GACTTAGAAA GAGCCAAGAG AAAACTGGAG 
GGTGACCTAA AATTGGCCCA AGAATCCACA ATGGATACAG AAAATGACAA ACAGCAACTT 
AATGAGAAAC TCAAAAAGAA AGAGTTTGAA ATGAGCAATC TGCAAGGCAA GATTGAAGAT 
GAACAAGCCC TTGCAATACA GCTACAAAAG AAGATCAAAG AATTACAGGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCCGGG CCAAAGCAGA GAAGCAGCGC 
TCTGACCTCT CCCGGGAGCT GGAGGAGATC AGTGAGAGGC TGGAAGAAGC CGGTGGGGCC 
ACTTCAGCCC AGATTGAGAT GAACAAGAAG CGGGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAAG AGTCCACCCT GCAGCACGAA GCCACGGCAG CTGCTCTTCG GAAGAAGCAC 
GCAGATAGTG TGGCTGAGCT TGGGGAGCAG ATTGACAGCC TTCAGCGGGT CAAGCAGAAG 
CTGGAGAAGG AAAAGAGTGA GCTGAAGATG GAGATCAATG ACCTTGCTAG TAACATGGAG 
ACTGTCTCCA AAGCCAAGGC AAACTTTGAG AAAATGTGCC GCACCCTAGA GGACCAGCTT 
AGTGAAATAA AAACAAAGGA AGAAGAGCAA CAACGCTTAA TAAATGAGTT GTCAGCCCAG 
AAGGCACGTT TACACACAGA ATCAGGTGAG TTTTCACGAC AGCTAGATGA AAAAGATGCT 
ATGGTTTCTC AGCTATCCCG AGGCAAACAA GCATTTACAC AACAGATTGA AGAATTAAAG 
AGGCAGCTAG AAGAGGAGAC TAAGGCCAAG AGCACTCTGG CCCATGCCCT GCAGTCAGCC 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAGGAGG AGCAGGAAGC CAAGGCTGAG 
CTGCAGAGGG GAATGTCCAA GGCCAACAGT GAGGTTGCCC AGTGGAGGAC CAAGTACGAG 
ACGGACGCCA TCCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT AGCCCAGCGT 
CTGCAGGATG CAGAAGAACA TGTAGAAGCT GTGAATTCCA AATGTGCTTC TCTTGAAAAG 
ACAAAGCAGA GGCTACAGAA TGAAGTAGAG GACCTCATGA TTGATGTGGA ACGATCTAAT 
GCTGCCTGCA TAGCTCTCGA TAAGAAGCAA AGAAACTTTG ACAAGGTTCT GGCAGAATGG 
AAACAGAAGT ATGAGGAAAC TCAGGCTGAA CTTGAGGCCT CCCAGAAGGA GTCGCGTTCT 
CTCAGCACTG AGCTGTTCAA GGTGAAGAAT GCCTACGAGG AATCCCTGGA TCATCTTGAA 
ACTCTAAAGC GAGAGAATAA GAACTTACAA CAGGAGATTT CTGACCTGAC AGAGCAAATT 
GCAGAGGGTG GAAAGCATAT CCATGAACTG GAGAAAGTAA AGAAACAACT TGATCATGAG 
AAGAGTGAAC TACAGACTTC CCTAGAGGAA GCAGAGGCAT CTCTTGAGCA TGAAGAAGGC 
AAAATTCTTC GCATTCAACT TGAGCTAAAT CAGGTGAAAT CTGAGATTGA CCGAAAAATT 
GCTGAAAAAG ATGAAGAACT CGATCAGCTA AAGAGGAACC ATCTCAGAGT TGTGGAGTCA 
ATGCAGAGTA CACTGGATGC TGAGATCAGG AGCAGAAATG ATGCTCTGAG GATCAAGAAG 
AAGATGGAGG GAGATCTTAA TGAAATGGAA ATCCAGCTGA ACCATGCCAA CCGCCAGGCT 
GCTGAGGCAC TAAGGAATCT TAGAAACACA CAAGGAATAC TGAAGGACAC TCAGCTACAT 
TTGGATGATG CCATCAGAGG CCAAGATGAC CTTAAGGAAC AACTGGCAAT GGTTGAGCGC 
AGAGCTAACC TGATGCAGGC TGAAGTTGAA GAGCTCAGGG CATCCCTGGA ACGGACTGAG 
AGAGGCAGGA AAATGGCAGA GCAAGAGCTT CTGGATGCCA GTGAACGTGT GCAACTTCTG 
CACACTCAGA ACACCAGCCT GATCAACACC AAGAAGAAGC TGGAAACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAGGA CATCGTCCAG GAAGCCCGCA ATGCAGAGGA GAAGGCCAAG 
AAGGCCATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAACA GGACACCAGC 
GCCCACCTGG AGCGGATGAA GAAGAACATG GAGCAGACCG TGAAGGATCT GCAGCTCCGT 
CTGGATGAGG CTGAGCAGCT GGCGCTGAAG GGTGGGAAGA AGCAGATCCA GAAACTGGAG 
GCCAGGGTGA GAGAGCTTGA AAGTGAGGTG GAAAGTGAAC AGAAGCACAA TGTTGAGGCT 
GTCAAGGGTC TTCGCAAACA TGAGAGAAGA GTGAAGGAAC TCACTTACCA GACTGAGGAG 
GACCGCAAGA ATATTCTCAG GCTGCAGGAC TTGGTGGACA AATTGCAAAC CAAAGTCAAA 
GCTTACAAGA GACAAGCTGA AGAGGCTGAG GAACAATCCA ATGTCAACCT TGCCAAGTTC 
CGCAAGCTCC AGCACGAGCT GGAGGAGGCC AAGGAACGGG CTGACATTGC TGAGTCCCAA 
GTCAACAAGC TGAGAGTGAA GAGTCGGGAG GTTCACACAA AAGTCATAAG TGAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060003.2
CDS	112..5931
Translation	MSSDSEMAIFGEAAPFLRKSEKERIEAQNKPFDAKTSVFVVDPKESYVKAIVQSREGGKVTAKTEAGATVTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPASGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIEMLLITTNPYDFAFVSQGEITVPSIDDQEELMATDSAVDILGFTADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKSLCYPRVKVGNEFVTKGQTVQQVYNAVGALAKAIYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLAFLFSGAQTAEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIEIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQAICRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLCMDLERAKRKLEGDLKLAQESTMDTENDKQQLNEKLKKKEFEMSNLQGKIEDEQALAIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEESTLQHEATAAALRKKHADSVAELGEQIDSLQRVKQKLEKEKSELKMEINDLASNMETVSKAKANFEKMCRTLEDQLSEIKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEETKAKSTLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACIALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVESMQSTLDAEIRSRNDALRIKKKMEGDLNEMEIQLNHANRQAAEALRNLRNTQGILKDTQLHLDDAIRGQDDLKEQLAMVERRANLMQAEVEELRASLERTERGRKMAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELESEVESEQKHNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKFRKLQHELEEAKERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	NM_017533.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 4 (MYH4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017533.2

ORF Insert Sequence:

ATGAGTTCTG ACTCTGAGAT GGCCATTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAAGGAGC GAATTGAAGC TCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTGGACCCTA AGGAGTCCTA CGTGAAAGCA ATAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGCTGG AGCTACTGTA ACTGTGAAAG AAGACCAAGT CTTCTCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCATGAGCCT 
GCTGTGCTGT ATAACCTCAA AGAGCGTTAC GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC TGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ATATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT AACTGATCGT GAAAACCAGT CAATCTTGAT TACTGGAGAA 
TCTGGTGCAG GGAAGACTGT GAACACGAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGAG AGAAGAAAAA AGAGGAACCT GCCTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAAATCA TCAGTGCTAA CCCCCTACTG GAAGCCTTCG GCAATGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ATTTTGGTGC CACAGGCAAA 
CTGGCTTCTG CAGATATTGA AACATATCTG CTAGAGAAGT CCCGAGTTAC TTTTCAGCTA 
AAGGCTGAAA GAAGCTACCA CATATTTTAT CAAATCCTGT CCAATAAGAA ACCAGAGCTC 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTTCG CATTTGTCAG CCAAGGGGAA 
ATTACTGTGC CCAGCATTGA TGACCAGGAA GAGCTGATGG CCACAGATAG TGCTGTGGAC 
ATCCTGGGTT TCACTGCTGA TGAAAAGGTG GCCATTTACA AGCTCACTGG AGCCGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAAAGGGAAG AGCAGGCAGA GCCAGATGGC 
ACGGAAGTTG CTGACAAAGC TGCTTATCTG ACAAGTCTGA ACTCTGCTGA CCTGCTCAAA 
TCTCTCTGCT ATCCCAGAGT CAAGGTCGGC AATGAGTTCG TAACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAATGCAGT GGGTGCTCTG GCCAAAGCCA TCTACGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAAGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT AGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAACATCTT 
GGAAAATCCA ACAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGCCTGA GGCTCACTTC 
TCACTGGTGC ACTATGCCGG CACCGTGGAC TACAACATCG CCGGCTGGCT GGACAAAAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTG 
GCTTTCCTCT TCTCTGGGGC ACAAACTGCT GAAGCAGAGG GTGGTGGTGG AAAGAAAGGT 
GGCAAAAAGA AGGGTTCTTC TTTCCAGACA GTGTCAGCTC TTTTCAGGGA GAATTTGAAT 
AAGCTGATGA CCAACTTGAG GAGCACTCAC CCCCACTTTG TGCGGTGCAT CATCCCCAAT 
GAAACTAAAA CTCCTGGTGC CATGGAGCAT GAGCTTGTCC TGCATCAGCT GAGGTGTAAC 
GGTGTGCTGG AAGGCATCCG CATCTGCAGG AAAGGCTTCC CAAGCAGAAT CCTTTATGCA 
GACTTCAAAC AGAGATACAA GGTTCTAAAT GCGAGTGCTA TCCCAGAGGG TCAGTTCATT 
GACAGCAAGA AGGCTTCTGA GAAACTTCTA GGGTCTATTG AAATTGACCA CACCCAGTAC 
AAATTCGGTC ATACCAAGGT TTTCTTCAAA GCTGGCCTGC TGGGAACTCT AGAGGAAATG 
CGAGATGAAA AGCTAGCTCA ACTCATCACG CGCACTCAAG CCATATGCAG AGGGTTCCTG 
ATGAGAGTGG AGTTCAGAAA GATGATGGAG AGGAGAGAGT CCATCTTCTG CATTCAGTAC 
AACATCCGTG CTTTCATGAA TGTGAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAGCCCCTCC TCAAGAGTGC AGAGACAGAG AAGGAGATGG CCAACATGAA GGAAGAATTT 
GAGAAAACCA AAGAAGAGCT GGCTAAGACA GAGGCAAAAA GGAAAGAACT AGAAGAAAAG 
ATGGTGACGC TAATGCAAGA GAAAAATGAC TTACAACTCC AAGTTCAAGC TGAAGCAGAT 
GCCTTGGCTG ATGCAGAGGA AAGATGTGAT CAGTTGATTA AAACCAAAAT CCAACTTGAG 
GCCAAAATCA AAGAGGTAAC TGAAAGAGCT GAGGATGAGG AAGAGATCAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAGC TCAAGAAAGA CATTGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAACATG CCACAGAGAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC AGGTCTGGAT GAAACCATTG CTAAGCTGAC CAAGGAGAAG 
AAGGCTCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGATGGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC TAAAACCAAG CTAGAACAGC AAGTGGACGA TCTTGAAGGA 
TCTCTGGAAC AAGAAAAGAA ACTTTGCATG GACTTAGAAA GAGCCAAGAG AAAACTGGAG 
GGTGACCTAA AATTGGCCCA AGAATCCACA ATGGATACAG AAAATGACAA ACAGCAACTT 
AATGAGAAAC TCAAAAAGAA AGAGTTTGAA ATGAGCAATC TGCAAGGCAA GATTGAAGAT 
GAACAAGCCC TTGCAATACA GCTACAAAAG AAGATCAAAG AATTACAGGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCCGGG CCAAAGCAGA GAAGCAGCGC 
TCTGACCTCT CCCGGGAGCT GGAGGAGATC AGTGAGAGGC TGGAAGAAGC CGGTGGGGCC 
ACTTCAGCCC AGATTGAGAT GAACAAGAAG CGGGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAAG AGTCCACCCT GCAGCACGAA GCCACGGCAG CTGCTCTTCG GAAGAAGCAC 
GCAGATAGTG TGGCTGAGCT TGGGGAGCAG ATTGACAGCC TTCAGCGGGT CAAGCAGAAG 
CTGGAGAAGG AAAAGAGTGA GCTGAAGATG GAGATCAATG ACCTTGCTAG TAACATGGAG 
ACTGTCTCCA AAGCCAAGGC AAACTTTGAG AAAATGTGCC GCACCCTAGA GGACCAGCTT 
AGTGAAATAA AAACAAAGGA AGAAGAGCAA CAACGCTTAA TAAATGAGTT GTCAGCCCAG 
AAGGCACGTT TACACACAGA ATCAGGTGAG TTTTCACGAC AGCTAGATGA AAAAGATGCT 
ATGGTTTCTC AGCTATCCCG AGGCAAACAA GCATTTACAC AACAGATTGA AGAATTAAAG 
AGGCAGCTAG AAGAGGAGAC TAAGGCCAAG AGCACTCTGG CCCATGCCCT GCAGTCAGCC 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAGGAGG AGCAGGAAGC CAAGGCTGAG 
CTGCAGAGGG GAATGTCCAA GGCCAACAGT GAGGTTGCCC AGTGGAGGAC CAAGTACGAG 
ACGGACGCCA TCCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT AGCCCAGCGT 
CTGCAGGATG CAGAAGAACA TGTAGAAGCT GTGAATTCCA AATGTGCTTC TCTTGAAAAG 
ACAAAGCAGA GGCTACAGAA TGAAGTAGAG GACCTCATGA TTGATGTGGA ACGATCTAAT 
GCTGCCTGCA TAGCTCTCGA TAAGAAGCAA AGAAACTTTG ACAAGGTTCT GGCAGAATGG 
AAACAGAAGT ATGAGGAAAC TCAGGCTGAA CTTGAGGCCT CCCAGAAGGA GTCGCGTTCT 
CTCAGCACTG AGCTGTTCAA GGTGAAGAAT GCCTACGAGG AATCCCTGGA TCATCTTGAA 
ACTCTAAAGC GAGAGAATAA GAACTTACAA CAGGAGATTT CTGACCTGAC AGAGCAAATT 
GCAGAGGGTG GAAAGCATAT CCATGAACTG GAGAAAGTAA AGAAACAACT TGATCATGAG 
AAGAGTGAAC TACAGACTTC CCTAGAGGAA GCAGAGGCAT CTCTTGAGCA TGAAGAAGGC 
AAAATTCTTC GCATTCAACT TGAGCTAAAT CAGGTGAAAT CTGAGATTGA CCGAAAAATT 
GCTGAAAAAG ATGAAGAACT CGATCAGCTA AAGAGGAACC ATCTCAGAGT TGTGGAGTCA 
ATGCAGAGTA CACTGGATGC TGAGATCAGG AGCAGAAATG ATGCTCTGAG GATCAAGAAG 
AAGATGGAGG GAGATCTTAA TGAAATGGAA ATCCAGCTGA ACCATGCCAA CCGCCAGGCT 
GCTGAGGCAC TAAGGAATCT TAGAAACACA CAAGGAATAC TGAAGGACAC TCAGCTACAT 
TTGGATGATG CCATCAGAGG CCAAGATGAC CTTAAGGAAC AACTGGCAAT GGTTGAGCGC 
AGAGCTAACC TGATGCAGGC TGAAGTTGAA GAGCTCAGGG CATCCCTGGA ACGGACTGAG 
AGAGGCAGGA AAATGGCAGA GCAAGAGCTT CTGGATGCCA GTGAACGTGT GCAACTTCTG 
CACACTCAGA ACACCAGCCT GATCAACACC AAGAAGAAGC TGGAAACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAGGA CATCGTCCAG GAAGCCCGCA ATGCAGAGGA GAAGGCCAAG 
AAGGCCATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAACA GGACACCAGC 
GCCCACCTGG AGCGGATGAA GAAGAACATG GAGCAGACCG TGAAGGATCT GCAGCTCCGT 
CTGGATGAGG CTGAGCAGCT GGCGCTGAAG GGTGGGAAGA AGCAGATCCA GAAACTGGAG 
GCCAGGGTGA GAGAGCTTGA AAGTGAGGTG GAAAGTGAAC AGAAGCACAA TGTTGAGGCT 
GTCAAGGGTC TTCGCAAACA TGAGAGAAGA GTGAAGGAAC TCACTTACCA GACTGAGGAG 
GACCGCAAGA ATATTCTCAG GCTGCAGGAC TTGGTGGACA AATTGCAAAC CAAAGTCAAA 
GCTTACAAGA GACAAGCTGA AGAGGCTGAG GAACAATCCA ATGTCAACCT TGCCAAGTTC 
CGCAAGCTCC AGCACGAGCT GGAGGAGGCC AAGGAACGGG CTGACATTGC TGAGTCCCAA 
GTCAACAAGC TGAGAGTGAA GAGTCGGGAG GTTCACACAA AAGTCATAAG TGAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH4 ( XM_017024676.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880165.1 Homo sapiens myosin heavy chain 4 (MYH4), transcript variant X1, mRNA.

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CloneID	OHu02368
Clone ID Related Accession (Same CDS sequence)	NM_017533.2 , XM_017024676.1
Accession Version	XM_017024676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	myosin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCTG ACTCTGAGAT GGCCATTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAAGGAGC GAATTGAAGC TCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTGGACCCTA AGGAGTCCTA CGTGAAAGCA ATAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGCTGG AGCTACTGTA ACTGTGAAAG AAGACCAAGT CTTCTCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCATGAGCCT 
GCTGTGCTGT ATAACCTCAA AGAGCGTTAC GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC TGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ATATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT AACTGATCGT GAAAACCAGT CAATCTTGAT TACTGGAGAA 
TCTGGTGCAG GGAAGACTGT GAACACGAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGAG AGAAGAAAAA AGAGGAACCT GCCTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAAATCA TCAGTGCTAA CCCCCTACTG GAAGCCTTCG GCAATGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ATTTTGGTGC CACAGGCAAA 
CTGGCTTCTG CAGATATTGA AACATATCTG CTAGAGAAGT CCCGAGTTAC TTTTCAGCTA 
AAGGCTGAAA GAAGCTACCA CATATTTTAT CAAATCCTGT CCAATAAGAA ACCAGAGCTC 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTTCG CATTTGTCAG CCAAGGGGAA 
ATTACTGTGC CCAGCATTGA TGACCAGGAA GAGCTGATGG CCACAGATAG TGCTGTGGAC 
ATCCTGGGTT TCACTGCTGA TGAAAAGGTG GCCATTTACA AGCTCACTGG AGCCGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAAAGGGAAG AGCAGGCAGA GCCAGATGGC 
ACGGAAGTTG CTGACAAAGC TGCTTATCTG ACAAGTCTGA ACTCTGCTGA CCTGCTCAAA 
TCTCTCTGCT ATCCCAGAGT CAAGGTCGGC AATGAGTTCG TAACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAATGCAGT GGGTGCTCTG GCCAAAGCCA TCTACGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAAGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT AGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAACATCTT 
GGAAAATCCA ACAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGCCTGA GGCTCACTTC 
TCACTGGTGC ACTATGCCGG CACCGTGGAC TACAACATCG CCGGCTGGCT GGACAAAAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTG 
GCTTTCCTCT TCTCTGGGGC ACAAACTGCT GAAGCAGAGG GTGGTGGTGG AAAGAAAGGT 
GGCAAAAAGA AGGGTTCTTC TTTCCAGACA GTGTCAGCTC TTTTCAGGGA GAATTTGAAT 
AAGCTGATGA CCAACTTGAG GAGCACTCAC CCCCACTTTG TGCGGTGCAT CATCCCCAAT 
GAAACTAAAA CTCCTGGTGC CATGGAGCAT GAGCTTGTCC TGCATCAGCT GAGGTGTAAC 
GGTGTGCTGG AAGGCATCCG CATCTGCAGG AAAGGCTTCC CAAGCAGAAT CCTTTATGCA 
GACTTCAAAC AGAGATACAA GGTTCTAAAT GCGAGTGCTA TCCCAGAGGG TCAGTTCATT 
GACAGCAAGA AGGCTTCTGA GAAACTTCTA GGGTCTATTG AAATTGACCA CACCCAGTAC 
AAATTCGGTC ATACCAAGGT TTTCTTCAAA GCTGGCCTGC TGGGAACTCT AGAGGAAATG 
CGAGATGAAA AGCTAGCTCA ACTCATCACG CGCACTCAAG CCATATGCAG AGGGTTCCTG 
ATGAGAGTGG AGTTCAGAAA GATGATGGAG AGGAGAGAGT CCATCTTCTG CATTCAGTAC 
AACATCCGTG CTTTCATGAA TGTGAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAGCCCCTCC TCAAGAGTGC AGAGACAGAG AAGGAGATGG CCAACATGAA GGAAGAATTT 
GAGAAAACCA AAGAAGAGCT GGCTAAGACA GAGGCAAAAA GGAAAGAACT AGAAGAAAAG 
ATGGTGACGC TAATGCAAGA GAAAAATGAC TTACAACTCC AAGTTCAAGC TGAAGCAGAT 
GCCTTGGCTG ATGCAGAGGA AAGATGTGAT CAGTTGATTA AAACCAAAAT CCAACTTGAG 
GCCAAAATCA AAGAGGTAAC TGAAAGAGCT GAGGATGAGG AAGAGATCAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAGC TCAAGAAAGA CATTGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAACATG CCACAGAGAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC AGGTCTGGAT GAAACCATTG CTAAGCTGAC CAAGGAGAAG 
AAGGCTCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGATGGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC TAAAACCAAG CTAGAACAGC AAGTGGACGA TCTTGAAGGA 
TCTCTGGAAC AAGAAAAGAA ACTTTGCATG GACTTAGAAA GAGCCAAGAG AAAACTGGAG 
GGTGACCTAA AATTGGCCCA AGAATCCACA ATGGATACAG AAAATGACAA ACAGCAACTT 
AATGAGAAAC TCAAAAAGAA AGAGTTTGAA ATGAGCAATC TGCAAGGCAA GATTGAAGAT 
GAACAAGCCC TTGCAATACA GCTACAAAAG AAGATCAAAG AATTACAGGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCCGGG CCAAAGCAGA GAAGCAGCGC 
TCTGACCTCT CCCGGGAGCT GGAGGAGATC AGTGAGAGGC TGGAAGAAGC CGGTGGGGCC 
ACTTCAGCCC AGATTGAGAT GAACAAGAAG CGGGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAAG AGTCCACCCT GCAGCACGAA GCCACGGCAG CTGCTCTTCG GAAGAAGCAC 
GCAGATAGTG TGGCTGAGCT TGGGGAGCAG ATTGACAGCC TTCAGCGGGT CAAGCAGAAG 
CTGGAGAAGG AAAAGAGTGA GCTGAAGATG GAGATCAATG ACCTTGCTAG TAACATGGAG 
ACTGTCTCCA AAGCCAAGGC AAACTTTGAG AAAATGTGCC GCACCCTAGA GGACCAGCTT 
AGTGAAATAA AAACAAAGGA AGAAGAGCAA CAACGCTTAA TAAATGAGTT GTCAGCCCAG 
AAGGCACGTT TACACACAGA ATCAGGTGAG TTTTCACGAC AGCTAGATGA AAAAGATGCT 
ATGGTTTCTC AGCTATCCCG AGGCAAACAA GCATTTACAC AACAGATTGA AGAATTAAAG 
AGGCAGCTAG AAGAGGAGAC TAAGGCCAAG AGCACTCTGG CCCATGCCCT GCAGTCAGCC 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAGGAGG AGCAGGAAGC CAAGGCTGAG 
CTGCAGAGGG GAATGTCCAA GGCCAACAGT GAGGTTGCCC AGTGGAGGAC CAAGTACGAG 
ACGGACGCCA TCCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT AGCCCAGCGT 
CTGCAGGATG CAGAAGAACA TGTAGAAGCT GTGAATTCCA AATGTGCTTC TCTTGAAAAG 
ACAAAGCAGA GGCTACAGAA TGAAGTAGAG GACCTCATGA TTGATGTGGA ACGATCTAAT 
GCTGCCTGCA TAGCTCTCGA TAAGAAGCAA AGAAACTTTG ACAAGGTTCT GGCAGAATGG 
AAACAGAAGT ATGAGGAAAC TCAGGCTGAA CTTGAGGCCT CCCAGAAGGA GTCGCGTTCT 
CTCAGCACTG AGCTGTTCAA GGTGAAGAAT GCCTACGAGG AATCCCTGGA TCATCTTGAA 
ACTCTAAAGC GAGAGAATAA GAACTTACAA CAGGAGATTT CTGACCTGAC AGAGCAAATT 
GCAGAGGGTG GAAAGCATAT CCATGAACTG GAGAAAGTAA AGAAACAACT TGATCATGAG 
AAGAGTGAAC TACAGACTTC CCTAGAGGAA GCAGAGGCAT CTCTTGAGCA TGAAGAAGGC 
AAAATTCTTC GCATTCAACT TGAGCTAAAT CAGGTGAAAT CTGAGATTGA CCGAAAAATT 
GCTGAAAAAG ATGAAGAACT CGATCAGCTA AAGAGGAACC ATCTCAGAGT TGTGGAGTCA 
ATGCAGAGTA CACTGGATGC TGAGATCAGG AGCAGAAATG ATGCTCTGAG GATCAAGAAG 
AAGATGGAGG GAGATCTTAA TGAAATGGAA ATCCAGCTGA ACCATGCCAA CCGCCAGGCT 
GCTGAGGCAC TAAGGAATCT TAGAAACACA CAAGGAATAC TGAAGGACAC TCAGCTACAT 
TTGGATGATG CCATCAGAGG CCAAGATGAC CTTAAGGAAC AACTGGCAAT GGTTGAGCGC 
AGAGCTAACC TGATGCAGGC TGAAGTTGAA GAGCTCAGGG CATCCCTGGA ACGGACTGAG 
AGAGGCAGGA AAATGGCAGA GCAAGAGCTT CTGGATGCCA GTGAACGTGT GCAACTTCTG 
CACACTCAGA ACACCAGCCT GATCAACACC AAGAAGAAGC TGGAAACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAGGA CATCGTCCAG GAAGCCCGCA ATGCAGAGGA GAAGGCCAAG 
AAGGCCATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAACA GGACACCAGC 
GCCCACCTGG AGCGGATGAA GAAGAACATG GAGCAGACCG TGAAGGATCT GCAGCTCCGT 
CTGGATGAGG CTGAGCAGCT GGCGCTGAAG GGTGGGAAGA AGCAGATCCA GAAACTGGAG 
GCCAGGGTGA GAGAGCTTGA AAGTGAGGTG GAAAGTGAAC AGAAGCACAA TGTTGAGGCT 
GTCAAGGGTC TTCGCAAACA TGAGAGAAGA GTGAAGGAAC TCACTTACCA GACTGAGGAG 
GACCGCAAGA ATATTCTCAG GCTGCAGGAC TTGGTGGACA AATTGCAAAC CAAAGTCAAA 
GCTTACAAGA GACAAGCTGA AGAGGCTGAG GAACAATCCA ATGTCAACCT TGCCAAGTTC 
CGCAAGCTCC AGCACGAGCT GGAGGAGGCC AAGGAACGGG CTGACATTGC TGAGTCCCAA 
GTCAACAAGC TGAGAGTGAA GAGTCGGGAG GTTCACACAA AAGTCATAAG TGAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880165.1
CDS	256..6075
Translation	MSSDSEMAIFGEAAPFLRKSEKERIEAQNKPFDAKTSVFVVDPKESYVKAIVQSREGGKVTAKTEAGATVTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPASGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIEMLLITTNPYDFAFVSQGEITVPSIDDQEELMATDSAVDILGFTADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKSLCYPRVKVGNEFVTKGQTVQQVYNAVGALAKAIYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLAFLFSGAQTAEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIEIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQAICRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLCMDLERAKRKLEGDLKLAQESTMDTENDKQQLNEKLKKKEFEMSNLQGKIEDEQALAIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEESTLQHEATAAALRKKHADSVAELGEQIDSLQRVKQKLEKEKSELKMEINDLASNMETVSKAKANFEKMCRTLEDQLSEIKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEETKAKSTLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACIALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVESMQSTLDAEIRSRNDALRIKKKMEGDLNEMEIQLNHANRQAAEALRNLRNTQGILKDTQLHLDDAIRGQDDLKEQLAMVERRANLMQAEVEELRASLERTERGRKMAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELESEVESEQKHNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKFRKLQHELEEAKERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	XM_017024676.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 4 (MYH4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024676.1

ORF Insert Sequence:

ATGAGTTCTG ACTCTGAGAT GGCCATTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAAGGAGC GAATTGAAGC TCAGAACAAG CCTTTTGATG CCAAGACATC AGTCTTTGTG 
GTGGACCCTA AGGAGTCCTA CGTGAAAGCA ATAGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGCTGG AGCTACTGTA ACTGTGAAAG AAGACCAAGT CTTCTCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCATGAGCCT 
GCTGTGCTGT ATAACCTCAA AGAGCGTTAC GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC TGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ATATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTCATGCT AACTGATCGT GAAAACCAGT CAATCTTGAT TACTGGAGAA 
TCTGGTGCAG GGAAGACTGT GAACACGAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGAG AGAAGAAAAA AGAGGAACCT GCCTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAAATCA TCAGTGCTAA CCCCCTACTG GAAGCCTTCG GCAATGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ATTTTGGTGC CACAGGCAAA 
CTGGCTTCTG CAGATATTGA AACATATCTG CTAGAGAAGT CCCGAGTTAC TTTTCAGCTA 
AAGGCTGAAA GAAGCTACCA CATATTTTAT CAAATCCTGT CCAATAAGAA ACCAGAGCTC 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTTCG CATTTGTCAG CCAAGGGGAA 
ATTACTGTGC CCAGCATTGA TGACCAGGAA GAGCTGATGG CCACAGATAG TGCTGTGGAC 
ATCCTGGGTT TCACTGCTGA TGAAAAGGTG GCCATTTACA AGCTCACTGG AGCCGTGATG 
CATTATGGGA ACATGAAATT CAAGCAAAAG CAAAGGGAAG AGCAGGCAGA GCCAGATGGC 
ACGGAAGTTG CTGACAAAGC TGCTTATCTG ACAAGTCTGA ACTCTGCTGA CCTGCTCAAA 
TCTCTCTGCT ATCCCAGAGT CAAGGTCGGC AATGAGTTCG TAACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAATGCAGT GGGTGCTCTG GCCAAAGCCA TCTACGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAAGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT AGAAGAGGAG 
TGCATGTTCC CCAAGGCAAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAACATCTT 
GGAAAATCCA ACAACTTCCA GAAGCCCAAG CCTGCCAAAG GCAAGCCTGA GGCTCACTTC 
TCACTGGTGC ACTATGCCGG CACCGTGGAC TACAACATCG CCGGCTGGCT GGACAAAAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTGCAAT GAAGACTCTG 
GCTTTCCTCT TCTCTGGGGC ACAAACTGCT GAAGCAGAGG GTGGTGGTGG AAAGAAAGGT 
GGCAAAAAGA AGGGTTCTTC TTTCCAGACA GTGTCAGCTC TTTTCAGGGA GAATTTGAAT 
AAGCTGATGA CCAACTTGAG GAGCACTCAC CCCCACTTTG TGCGGTGCAT CATCCCCAAT 
GAAACTAAAA CTCCTGGTGC CATGGAGCAT GAGCTTGTCC TGCATCAGCT GAGGTGTAAC 
GGTGTGCTGG AAGGCATCCG CATCTGCAGG AAAGGCTTCC CAAGCAGAAT CCTTTATGCA 
GACTTCAAAC AGAGATACAA GGTTCTAAAT GCGAGTGCTA TCCCAGAGGG TCAGTTCATT 
GACAGCAAGA AGGCTTCTGA GAAACTTCTA GGGTCTATTG AAATTGACCA CACCCAGTAC 
AAATTCGGTC ATACCAAGGT TTTCTTCAAA GCTGGCCTGC TGGGAACTCT AGAGGAAATG 
CGAGATGAAA AGCTAGCTCA ACTCATCACG CGCACTCAAG CCATATGCAG AGGGTTCCTG 
ATGAGAGTGG AGTTCAGAAA GATGATGGAG AGGAGAGAGT CCATCTTCTG CATTCAGTAC 
AACATCCGTG CTTTCATGAA TGTGAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAGCCCCTCC TCAAGAGTGC AGAGACAGAG AAGGAGATGG CCAACATGAA GGAAGAATTT 
GAGAAAACCA AAGAAGAGCT GGCTAAGACA GAGGCAAAAA GGAAAGAACT AGAAGAAAAG 
ATGGTGACGC TAATGCAAGA GAAAAATGAC TTACAACTCC AAGTTCAAGC TGAAGCAGAT 
GCCTTGGCTG ATGCAGAGGA AAGATGTGAT CAGTTGATTA AAACCAAAAT CCAACTTGAG 
GCCAAAATCA AAGAGGTAAC TGAAAGAGCT GAGGATGAGG AAGAGATCAA TGCTGAGCTG 
ACAGCCAAGA AGAGGAAACT GGAGGATGAA TGTTCAGAGC TCAAGAAAGA CATTGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAACATG CCACAGAGAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC AGGTCTGGAT GAAACCATTG CTAAGCTGAC CAAGGAGAAG 
AAGGCTCTCC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGATGGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC TAAAACCAAG CTAGAACAGC AAGTGGACGA TCTTGAAGGA 
TCTCTGGAAC AAGAAAAGAA ACTTTGCATG GACTTAGAAA GAGCCAAGAG AAAACTGGAG 
GGTGACCTAA AATTGGCCCA AGAATCCACA ATGGATACAG AAAATGACAA ACAGCAACTT 
AATGAGAAAC TCAAAAAGAA AGAGTTTGAA ATGAGCAATC TGCAAGGCAA GATTGAAGAT 
GAACAAGCCC TTGCAATACA GCTACAAAAG AAGATCAAAG AATTACAGGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCCGGG CCAAAGCAGA GAAGCAGCGC 
TCTGACCTCT CCCGGGAGCT GGAGGAGATC AGTGAGAGGC TGGAAGAAGC CGGTGGGGCC 
ACTTCAGCCC AGATTGAGAT GAACAAGAAG CGGGAGGCTG AGTTCCAGAA AATGCGCAGG 
GACCTGGAAG AGTCCACCCT GCAGCACGAA GCCACGGCAG CTGCTCTTCG GAAGAAGCAC 
GCAGATAGTG TGGCTGAGCT TGGGGAGCAG ATTGACAGCC TTCAGCGGGT CAAGCAGAAG 
CTGGAGAAGG AAAAGAGTGA GCTGAAGATG GAGATCAATG ACCTTGCTAG TAACATGGAG 
ACTGTCTCCA AAGCCAAGGC AAACTTTGAG AAAATGTGCC GCACCCTAGA GGACCAGCTT 
AGTGAAATAA AAACAAAGGA AGAAGAGCAA CAACGCTTAA TAAATGAGTT GTCAGCCCAG 
AAGGCACGTT TACACACAGA ATCAGGTGAG TTTTCACGAC AGCTAGATGA AAAAGATGCT 
ATGGTTTCTC AGCTATCCCG AGGCAAACAA GCATTTACAC AACAGATTGA AGAATTAAAG 
AGGCAGCTAG AAGAGGAGAC TAAGGCCAAG AGCACTCTGG CCCATGCCCT GCAGTCAGCC 
CGCCATGACT GTGACCTGCT GCGGGAACAG TATGAGGAGG AGCAGGAAGC CAAGGCTGAG 
CTGCAGAGGG GAATGTCCAA GGCCAACAGT GAGGTTGCCC AGTGGAGGAC CAAGTACGAG 
ACGGACGCCA TCCAGCGCAC AGAGGAGCTG GAGGAGGCCA AGAAGAAGCT AGCCCAGCGT 
CTGCAGGATG CAGAAGAACA TGTAGAAGCT GTGAATTCCA AATGTGCTTC TCTTGAAAAG 
ACAAAGCAGA GGCTACAGAA TGAAGTAGAG GACCTCATGA TTGATGTGGA ACGATCTAAT 
GCTGCCTGCA TAGCTCTCGA TAAGAAGCAA AGAAACTTTG ACAAGGTTCT GGCAGAATGG 
AAACAGAAGT ATGAGGAAAC TCAGGCTGAA CTTGAGGCCT CCCAGAAGGA GTCGCGTTCT 
CTCAGCACTG AGCTGTTCAA GGTGAAGAAT GCCTACGAGG AATCCCTGGA TCATCTTGAA 
ACTCTAAAGC GAGAGAATAA GAACTTACAA CAGGAGATTT CTGACCTGAC AGAGCAAATT 
GCAGAGGGTG GAAAGCATAT CCATGAACTG GAGAAAGTAA AGAAACAACT TGATCATGAG 
AAGAGTGAAC TACAGACTTC CCTAGAGGAA GCAGAGGCAT CTCTTGAGCA TGAAGAAGGC 
AAAATTCTTC GCATTCAACT TGAGCTAAAT CAGGTGAAAT CTGAGATTGA CCGAAAAATT 
GCTGAAAAAG ATGAAGAACT CGATCAGCTA AAGAGGAACC ATCTCAGAGT TGTGGAGTCA 
ATGCAGAGTA CACTGGATGC TGAGATCAGG AGCAGAAATG ATGCTCTGAG GATCAAGAAG 
AAGATGGAGG GAGATCTTAA TGAAATGGAA ATCCAGCTGA ACCATGCCAA CCGCCAGGCT 
GCTGAGGCAC TAAGGAATCT TAGAAACACA CAAGGAATAC TGAAGGACAC TCAGCTACAT 
TTGGATGATG CCATCAGAGG CCAAGATGAC CTTAAGGAAC AACTGGCAAT GGTTGAGCGC 
AGAGCTAACC TGATGCAGGC TGAAGTTGAA GAGCTCAGGG CATCCCTGGA ACGGACTGAG 
AGAGGCAGGA AAATGGCAGA GCAAGAGCTT CTGGATGCCA GTGAACGTGT GCAACTTCTG 
CACACTCAGA ACACCAGCCT GATCAACACC AAGAAGAAGC TGGAAACAGA CATTTCCCAA 
ATCCAGGGAG AGATGGAGGA CATCGTCCAG GAAGCCCGCA ATGCAGAGGA GAAGGCCAAG 
AAGGCCATCA CTGATGCTGC CATGATGGCT GAGGAGCTGA AGAAGGAACA GGACACCAGC 
GCCCACCTGG AGCGGATGAA GAAGAACATG GAGCAGACCG TGAAGGATCT GCAGCTCCGT 
CTGGATGAGG CTGAGCAGCT GGCGCTGAAG GGTGGGAAGA AGCAGATCCA GAAACTGGAG 
GCCAGGGTGA GAGAGCTTGA AAGTGAGGTG GAAAGTGAAC AGAAGCACAA TGTTGAGGCT 
GTCAAGGGTC TTCGCAAACA TGAGAGAAGA GTGAAGGAAC TCACTTACCA GACTGAGGAG 
GACCGCAAGA ATATTCTCAG GCTGCAGGAC TTGGTGGACA AATTGCAAAC CAAAGTCAAA 
GCTTACAAGA GACAAGCTGA AGAGGCTGAG GAACAATCCA ATGTCAACCT TGCCAAGTTC 
CGCAAGCTCC AGCACGAGCT GGAGGAGGCC AAGGAACGGG CTGACATTGC TGAGTCCCAA 
GTCAACAAGC TGAGAGTGAA GAGTCGGGAG GTTCACACAA AAGTCATAAG TGAAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation.
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery272(10)2915-23(2015 Oct)
Qiu X,Chen D,Li M,Gao Y,Liu F,Zheng H,Chen S

Myosin heavy chain-2b transcripts and isoform are expressed in human laryngeal muscles.
Cells, tissues, organs198(1)75-86(2013)
Smerdu V,Cvetko E

Temporal spatial expression and function of non-muscle myosin II isoforms IIA and IIB in scar remodeling.
Laboratory investigation; a journal of technical methods and pathology91(4)499-508(2011 Apr)
Bond JE,Ho TQ,Selim MA,Hunter CL,Bowers EV,Levinson H

Mts1 regulates the assembly of nonmuscle myosin-IIA.
Biochemistry42(48)14258-66(2003 Dec)
Li ZH,Spektor A,Varlamova O,Bresnick AR

Abundant expression of myosin heavy-chain IIB RNA in a subset of human masseter muscle fibres.
Archives of oral biology46(11)1039-50(2001 Nov)
Horton MJ,Brandon CA,Morris TJ,Braun TW,Yaw KM,Sciote JJ

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MYH4 cDNA ORF clone, Homo sapiens(human)

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