myha cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	myha
Entrez Gene ID	100149148
Full Name	myosin, heavy chain a
Synonyms	si:ch211-251m3.3
General protein information	Preferred Names myosin, heavy chain a Names myosin heavy chain, fast skeletal muscle fmyhc2.3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001923974.6	XP_001924009.1	myosin heavy chain, fast skeletal muscle
XM_021476518.1	XP_021332193.1	myosin heavy chain, fast skeletal muscle

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH4	XP_523785.2
Macaca mulatta (Rhesus monkey)	MYH4	XP_002800337.1
Canis lupus familiaris (dog)	MYH4	NP_001070262.1
Danio rerio (zebrafish)	myhz2	NP_694514.2
Danio rerio (zebrafish)	myhz1.2	NP_001154918.1
Xenopus tropicalis (tropical clawed frog)	myh3	NP_001006915.1
Danio rerio (zebrafish)	myhb	XP_001339206.5
Homo sapiens (human)	MYH4	NP_060003.2
Rattus norvegicus (Norway rat)	Myh4	NP_062198.1
Danio rerio (zebrafish)	zgc:66156	XP_002666141.3
Danio rerio (zebrafish)	ns:zf-e68	XP_001924014.2
Bos taurus (cattle)	MYH4	XP_005220494.1
Mus musculus (house mouse)	Myh4	NP_034985.2
Danio rerio (zebrafish)	myhc4	NP_001018321.1
Danio rerio (zebrafish)	myhz1.1	NP_001108561.1
Danio rerio (zebrafish)	wu:fd14a01	XP_002667379.3
Danio rerio (zebrafish)	myha	XP_001924009.1
Xenopus tropicalis (tropical clawed frog)	myh7	NP_001135564.1
Xenopus tropicalis (tropical clawed frog)	LOC100492956	XP_002937039.1
Xenopus tropicalis (tropical clawed frog)	LOC100493121	XP_004919657.1

Gene Ontology

The following myha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa10615	XM_001923974.6 Latest version!	Danio rerio myosin, heavy chain a (myha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa10615	XM_021476518.1 Latest version!	Danio rerio myosin, heavy chain a (myha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myha ( XM_001923974.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001924009.1 Danio rerio myosin, heavy chain a (myha), mRNA.

Price & Availability↑

CloneID	ODa10615
Clone ID Related Accession (Same CDS sequence)	XM_001923974.6 , XM_021476518.1
Accession Version	XM_001923974.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	myosin heavy chain, fast skeletal muscle
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001923974.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAAA GAATTGAGGC TCAGAACAAA CCATTTGATG CCAAATCTGC TTGCTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGAAGA TGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGACTGTTCT GCGCCACTGT AAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA AAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCGGTGACAA GAAGAAAGAG CAGACTCCAG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG TACAACTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAAGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCAACTGA TACTGCTATT 
GACATTCTGG GCTTCACTGG TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCTCTTAC CTTCTGGGTT TGAACTCTGC TGAAATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGTTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCTATGGGAA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AGGCCTCAAA AAGGCAAGGC TGAGGCTCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACG TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTGTACC AGAAGTCTTC TGTCAAACTG 
TTGGCTACTC TCTACCCACC CGTCGTTGAG GACACTTCAA AGAAGGGAGG CAAGAAGAAG 
GGTGGTTCCA TGCAGACTGT GTCTTCTCAG TTCAGGGAGA ACTTGGGCAA GCTAATGACC 
AACTTGAGGA GCACTCACCC TCATTTTGTG CGTTGTCTGA TTCCCAATGA GTCCAAGACT 
CCAGGTCTTA TGGAGAACTT CCTAGTTATC CACCAGCTGA GGTGTAACGG TGTACTGGAG 
GGTATCAGAA TCTGTAGAAA GGGTTTCCCA AGCAGAATTC TTTATGCCGA CTTCAAGCAG 
AGATACAAGG TGTTGAATGC CAGTGTTATC CCAGAGGGAC AGTTCATTGA CAACAAGAAG 
GCCAGTGAGA AACTCCTGGG ATCTATTGAT GTTAATCATG ATGAGTACAG ATTTGGACAC 
ACAAAGGTGT TCTTCAAAGC TGGTCTGCTG GGTACTCTTG AGGAGATGCG TGATGAGAAA 
CTGGCTAGTC TGGTCACAAT GACTCAGGCT CTCTGCCGTG CTTATCTGAT GAGGAGAGAG 
TTTGTGAAGA TGATGGAGAG GAGGGAGTCC ATTTACACCA TCCAGTACAA CATCCGCTCA 
TTCATGAATG TCAAACACTG GCCATGGATG AAGGTTTACT ACAAGATTAA GCCTCTGCTG 
AAGAGTGCTG AGACTGAGAA AGAACTGGCA AACATGAAAG AGGACTTTGT CAAATGCAAA 
GAAGATCTTG TCAAGGCTGA AGCTAAAAAG AAGGAGCTGG AAGAGAAGAT GGTGGCACTG 
CTGCAAGAGA AAAATGATCT GCAGCTGGCA GTGGCTTCTG AAGCTGAAAA TCTCTCAGAT 
GCTGAGGAGA GGTGTGAGGG TCTGATCAAG AGCAAAATCC AGCTTGAGGC TAAACTCAAA 
GAGACAACTG AGAGACTGGA GGATGAGGAA GAAATCAATG CTGAACTGAC AGCCAAGAAG 
AGAAAACTGG AGGACGAGTG CTCTGAGCTG AAGAAAGACA TTGATGACCT GGAGCTCACC 
TTGGCTAAAG TGGAAAAGGA GAAACATGCC ACTGAGAATA AGGTTAAGAA CTTGACAGAG 
GAAATGGCAT CTCAGGATGA GAGCATTGCC AAGCTTACAA AGGAGAAGAA AGCCCTCCAA 
GAGGCACATC AGCAGACTCT GGATGATCTT CAGGCTGAGG AGGACAAAGT CAACACCCTG 
ACCAAATCCA AGACAAAGCT TGAGCAGCAA GTTGATGATC TTGAGGGTTC CCTTGAACAA 
GAAAAGAAGC TCCGTATGGA CCTGGAGAGA GCTAAGAGAA AGCTTGAAGG AGATCTAAAA 
TTGGCCCAAG AGTCTATCAT GGACCTGGAA AATGACAAGC AGCAGTCTGA AGAGAAAATT 
AAAAAGAAAG ACTTTGAAAC AAGCCAGCTG CTCAGCAAGA TTGAAGATGA ACAATCCTTG 
GGTGCTCAGC TCCAGAAAAA GATCAAGGAG CTTCAGGCTC GCATTGAGGA ACTGGAGGAA 
GAGATTGAGG CTGAGCGTGC TGCTCGTGCC AAGGTTGAGA AGCAGAGAGC AGATCTCTCC 
AGGGAACTTG AGGAGATCAG TGAGAGGCTT GAGGAGGCTG GAGGAGCCAC TGCTGCTCAA 
ATCGAGATGA ATAAGAAGCG TGAAGCTGAA TTCCAGAAGC TGCGTCGTGA TCTTGAAGAG 
TCCACCCTTC AGCATGAAGC TACTGCTGCT GCCCTGCGCA AGAAGCAGGC AGACAGTGTG 
GCTGAGCTGG GAGAGCAAAT CGACAACCTC CAGCGTGTCA AGCAGAAGCT TGAGAAAGAG 
AAGAGTGAAT ACAAAATGGA GATCGATGAT CTCTCCAGCA ATATGGAGGC TGTCGCCAAA 
GCAAAGGCCA ATCTTGAGAA GATGTGTCGC ACACTTGAGG ATCAACTTAG TGAAATAAAG 
TCCAAGAGTG ATGAGAACCT TCGCCAGATA AATGATCTCA GTGCTCAAAG AGCAAGACTT 
CAAACTGAGA ATGGTGAGTT TGGCCGTCAG CTGGAGGAGA AAGAAGCTCT GGTTTCTCAG 
CTCACAAGAG GAAAACAGGC TTTCACTCAG CAAATTGAGG AGCTTAAGAG GCAGATTGAA 
GAGGAGGTCA AGGCTAAGAA CGCACTGGCC CATGCTGTAC AATCAGCCCG TCATGACTGC 
GACCTGCTTC GTGAGCAGTT TGAGGAGGAG CAGGAAGCAA AGGCTGAGCT GCAGCGGGGA 
ATGTCAAAGG CAAACAGTGA GGTTGCTCAG TGGAGAACCA AATATGAAAC TGATGCCATC 
CAGCGCACAG AAGAACTTGA AGAATCCAAG AAGAAACTGG CTCAGCGTCT GCAAGAGGCA 
GAGGAACAAA TTGAGGCAGT GAACTCCAAA TGTGCATCTC TGGAGAAGAC CAAACAGAGA 
CTCCAGGGTG AAGTGGAGGA CCTCATGATT GATGTGGAGA GAGCCAATTC TTTGGCTGCC 
AACCTTGACA AAAAGCAGAG AAACTTTGAC AAGGTCCTGG CAGAATGGAA ACAGAAATAT 
GAGGAAGGTC AGGCAGAGCT GGAAGGTGCC CAGAAAGAAG CTCGTTCACT CAGCACTGAG 
CTGTTCAAGA TGAAGAACTC CTATGAGGAG ACTCTGGATC AGCTGGAGAC CCTCAAGAGA 
GAGAACAAGA ATCTGCAGCA GGAGATTTCA GATCTGACAG AGCAGATAGG TGAGACTGGT 
AAGAGCATCC ATGAGCTGGA AAAGGCCAAG AAGACAGTGG AGACTGAGAA GGCAGAGATT 
CAGACCGCCC TAGAAGAGGC TGAAGGCACC CTGGAGCATG AGGAGTCCAA GATTCTTCGT 
GTCCAGCTTG AGCTAAACCA GGTCAAGGGT GAGATTGACA GGAAGCTTGC AGAGAAGGAT 
GAGGAGATAG AGCAGATCAA GAGGAACAGT CAGAGAGTTA CTGAAGCCAT GCAGAGCACT 
CTGGACTCTG AAGTCAGAAG CAGGAATGAT GCTCTCAGAA TCAAGAAGAA GATGGAGGGA 
GACCTTAATG AAATGGAGAT TCAGCTGAGC CATGCCAACC GCCAGGCTGC TGAGGCCCAG 
AAACAGCTCA GGAATGTTCA GTCACAACTC AAGGATGCCC AGCTGCACCT TGATGATGCT 
GTGAGAGCAC AGGAAGACAT GAAGGAGCAG GTGGCCATGG TGGAGCGCAG AAACACTCTG 
ATGCAGTCTG AGATCGAAGA GCTGAGAGCT GCTCTGGAGC AGACAGAGAG AGGACGCAAA 
GTGGCTGAAC AAGAGCTGGT GGATGCCAGT GAGCGTGTTG GACTTTTGCA CTCTCAGAAC 
ACAAGTCTTC TGAACACGAA GAAGAAGCTT GAGTCTGACC TTGTTCAGAT CCAAAGTGAA 
GTTGAAGACA CTGTACAGGA AGCCAGAAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACT 
GATGCTGCAA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGTGC TCACCTTGAG 
AGGATGAAGA AGAATCTGGA GATCACAGTG AAAGACCTGC AGCACCGTCT GGATGAAGCT 
GAGAATCTGG CCATGAAGGG AGGAAAGAAA CAACTCCAGA AACTGGAGTC CAGAGTTCGT 
GAGCTTGAGA GCGAAATCGA TGCAGAGCAG AGACGTGGAG CTGATGCTGT TAAAGGCGTC 
CGCAAATATG AGAGGAGAGT CAAGGAGCTC ACCTACCAGA CTGAGGAGGA CAAGAAGAAC 
ATCAACAGGC TGCAGGATCT GGTTGATAAG CTTCAGCTGA AGGTCAAGGC TTACAAGAGA 
CAGTCTGAAG AAGCTGAGGA ACAAGCCAAC TCTCACCTGT CCAAGTTGAG GAAGGTGCAG 
CATGAGCTGG AGGAGGCTGA GGAGCGCGCT GACATTTCTG AATCCCAGGT CAACAAGCTC 
AGAGCCAAGA GCCGTGACGC TGGAAAGGCT AAGGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001924009.1
CDS	63..5864
Translation	MSTDAEMAVYGKAAIYLRKPEKERIEAQNKPFDAKSACYVVDDKELYVKGTIKSRDGGKVTVITLDTKEERVAKEEDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGGDKKKEQTPGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFTGEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKISYLLGLNSAEMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVIRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPRPQKGKAEAHFSLVHYAGTVDYNVSGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEDTSKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRAYLMRREFVKMMERRESIYTIQYNIRSFMNVKHWPWMKVYYKIKPLLKSAETEKELANMKEDFVKCKEDLVKAEAKKKELEEKMVALLQEKNDLQLAVASEAENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMASQDESIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKIKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKSKSDENLRQINDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANSLAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQIGETGKSIHELEKAKKTVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKGEIDRKLAEKDEEIEQIKRNSQRVTEAMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQSQLKDAQLHLDDAVRAQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLESDLVQIQSEVEDTVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEITVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEIDAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNINRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADISESQVNKLRAKSRDAGKAKEE

Target ORF information:

RefSeq Version	XM_001923974.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin, heavy chain a (myha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001923974.6

ORF Insert Sequence:

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAAA GAATTGAGGC TCAGAACAAA CCATTTGATG CCAAATCTGC TTGCTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGAAGA TGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGACTGTTCT GCGCCACTGT AAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA AAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCGGTGACAA GAAGAAAGAG CAGACTCCAG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG TACAACTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAAGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCAACTGA TACTGCTATT 
GACATTCTGG GCTTCACTGG TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCTCTTAC CTTCTGGGTT TGAACTCTGC TGAAATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGTTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCTATGGGAA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AGGCCTCAAA AAGGCAAGGC TGAGGCTCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACG TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTGTACC AGAAGTCTTC TGTCAAACTG 
TTGGCTACTC TCTACCCACC CGTCGTTGAG GACACTTCAA AGAAGGGAGG CAAGAAGAAG 
GGTGGTTCCA TGCAGACTGT GTCTTCTCAG TTCAGGGAGA ACTTGGGCAA GCTAATGACC 
AACTTGAGGA GCACTCACCC TCATTTTGTG CGTTGTCTGA TTCCCAATGA GTCCAAGACT 
CCAGGTCTTA TGGAGAACTT CCTAGTTATC CACCAGCTGA GGTGTAACGG TGTACTGGAG 
GGTATCAGAA TCTGTAGAAA GGGTTTCCCA AGCAGAATTC TTTATGCCGA CTTCAAGCAG 
AGATACAAGG TGTTGAATGC CAGTGTTATC CCAGAGGGAC AGTTCATTGA CAACAAGAAG 
GCCAGTGAGA AACTCCTGGG ATCTATTGAT GTTAATCATG ATGAGTACAG ATTTGGACAC 
ACAAAGGTGT TCTTCAAAGC TGGTCTGCTG GGTACTCTTG AGGAGATGCG TGATGAGAAA 
CTGGCTAGTC TGGTCACAAT GACTCAGGCT CTCTGCCGTG CTTATCTGAT GAGGAGAGAG 
TTTGTGAAGA TGATGGAGAG GAGGGAGTCC ATTTACACCA TCCAGTACAA CATCCGCTCA 
TTCATGAATG TCAAACACTG GCCATGGATG AAGGTTTACT ACAAGATTAA GCCTCTGCTG 
AAGAGTGCTG AGACTGAGAA AGAACTGGCA AACATGAAAG AGGACTTTGT CAAATGCAAA 
GAAGATCTTG TCAAGGCTGA AGCTAAAAAG AAGGAGCTGG AAGAGAAGAT GGTGGCACTG 
CTGCAAGAGA AAAATGATCT GCAGCTGGCA GTGGCTTCTG AAGCTGAAAA TCTCTCAGAT 
GCTGAGGAGA GGTGTGAGGG TCTGATCAAG AGCAAAATCC AGCTTGAGGC TAAACTCAAA 
GAGACAACTG AGAGACTGGA GGATGAGGAA GAAATCAATG CTGAACTGAC AGCCAAGAAG 
AGAAAACTGG AGGACGAGTG CTCTGAGCTG AAGAAAGACA TTGATGACCT GGAGCTCACC 
TTGGCTAAAG TGGAAAAGGA GAAACATGCC ACTGAGAATA AGGTTAAGAA CTTGACAGAG 
GAAATGGCAT CTCAGGATGA GAGCATTGCC AAGCTTACAA AGGAGAAGAA AGCCCTCCAA 
GAGGCACATC AGCAGACTCT GGATGATCTT CAGGCTGAGG AGGACAAAGT CAACACCCTG 
ACCAAATCCA AGACAAAGCT TGAGCAGCAA GTTGATGATC TTGAGGGTTC CCTTGAACAA 
GAAAAGAAGC TCCGTATGGA CCTGGAGAGA GCTAAGAGAA AGCTTGAAGG AGATCTAAAA 
TTGGCCCAAG AGTCTATCAT GGACCTGGAA AATGACAAGC AGCAGTCTGA AGAGAAAATT 
AAAAAGAAAG ACTTTGAAAC AAGCCAGCTG CTCAGCAAGA TTGAAGATGA ACAATCCTTG 
GGTGCTCAGC TCCAGAAAAA GATCAAGGAG CTTCAGGCTC GCATTGAGGA ACTGGAGGAA 
GAGATTGAGG CTGAGCGTGC TGCTCGTGCC AAGGTTGAGA AGCAGAGAGC AGATCTCTCC 
AGGGAACTTG AGGAGATCAG TGAGAGGCTT GAGGAGGCTG GAGGAGCCAC TGCTGCTCAA 
ATCGAGATGA ATAAGAAGCG TGAAGCTGAA TTCCAGAAGC TGCGTCGTGA TCTTGAAGAG 
TCCACCCTTC AGCATGAAGC TACTGCTGCT GCCCTGCGCA AGAAGCAGGC AGACAGTGTG 
GCTGAGCTGG GAGAGCAAAT CGACAACCTC CAGCGTGTCA AGCAGAAGCT TGAGAAAGAG 
AAGAGTGAAT ACAAAATGGA GATCGATGAT CTCTCCAGCA ATATGGAGGC TGTCGCCAAA 
GCAAAGGCCA ATCTTGAGAA GATGTGTCGC ACACTTGAGG ATCAACTTAG TGAAATAAAG 
TCCAAGAGTG ATGAGAACCT TCGCCAGATA AATGATCTCA GTGCTCAAAG AGCAAGACTT 
CAAACTGAGA ATGGTGAGTT TGGCCGTCAG CTGGAGGAGA AAGAAGCTCT GGTTTCTCAG 
CTCACAAGAG GAAAACAGGC TTTCACTCAG CAAATTGAGG AGCTTAAGAG GCAGATTGAA 
GAGGAGGTCA AGGCTAAGAA CGCACTGGCC CATGCTGTAC AATCAGCCCG TCATGACTGC 
GACCTGCTTC GTGAGCAGTT TGAGGAGGAG CAGGAAGCAA AGGCTGAGCT GCAGCGGGGA 
ATGTCAAAGG CAAACAGTGA GGTTGCTCAG TGGAGAACCA AATATGAAAC TGATGCCATC 
CAGCGCACAG AAGAACTTGA AGAATCCAAG AAGAAACTGG CTCAGCGTCT GCAAGAGGCA 
GAGGAACAAA TTGAGGCAGT GAACTCCAAA TGTGCATCTC TGGAGAAGAC CAAACAGAGA 
CTCCAGGGTG AAGTGGAGGA CCTCATGATT GATGTGGAGA GAGCCAATTC TTTGGCTGCC 
AACCTTGACA AAAAGCAGAG AAACTTTGAC AAGGTCCTGG CAGAATGGAA ACAGAAATAT 
GAGGAAGGTC AGGCAGAGCT GGAAGGTGCC CAGAAAGAAG CTCGTTCACT CAGCACTGAG 
CTGTTCAAGA TGAAGAACTC CTATGAGGAG ACTCTGGATC AGCTGGAGAC CCTCAAGAGA 
GAGAACAAGA ATCTGCAGCA GGAGATTTCA GATCTGACAG AGCAGATAGG TGAGACTGGT 
AAGAGCATCC ATGAGCTGGA AAAGGCCAAG AAGACAGTGG AGACTGAGAA GGCAGAGATT 
CAGACCGCCC TAGAAGAGGC TGAAGGCACC CTGGAGCATG AGGAGTCCAA GATTCTTCGT 
GTCCAGCTTG AGCTAAACCA GGTCAAGGGT GAGATTGACA GGAAGCTTGC AGAGAAGGAT 
GAGGAGATAG AGCAGATCAA GAGGAACAGT CAGAGAGTTA CTGAAGCCAT GCAGAGCACT 
CTGGACTCTG AAGTCAGAAG CAGGAATGAT GCTCTCAGAA TCAAGAAGAA GATGGAGGGA 
GACCTTAATG AAATGGAGAT TCAGCTGAGC CATGCCAACC GCCAGGCTGC TGAGGCCCAG 
AAACAGCTCA GGAATGTTCA GTCACAACTC AAGGATGCCC AGCTGCACCT TGATGATGCT 
GTGAGAGCAC AGGAAGACAT GAAGGAGCAG GTGGCCATGG TGGAGCGCAG AAACACTCTG 
ATGCAGTCTG AGATCGAAGA GCTGAGAGCT GCTCTGGAGC AGACAGAGAG AGGACGCAAA 
GTGGCTGAAC AAGAGCTGGT GGATGCCAGT GAGCGTGTTG GACTTTTGCA CTCTCAGAAC 
ACAAGTCTTC TGAACACGAA GAAGAAGCTT GAGTCTGACC TTGTTCAGAT CCAAAGTGAA 
GTTGAAGACA CTGTACAGGA AGCCAGAAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACT 
GATGCTGCAA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGTGC TCACCTTGAG 
AGGATGAAGA AGAATCTGGA GATCACAGTG AAAGACCTGC AGCACCGTCT GGATGAAGCT 
GAGAATCTGG CCATGAAGGG AGGAAAGAAA CAACTCCAGA AACTGGAGTC CAGAGTTCGT 
GAGCTTGAGA GCGAAATCGA TGCAGAGCAG AGACGTGGAG CTGATGCTGT TAAAGGCGTC 
CGCAAATATG AGAGGAGAGT CAAGGAGCTC ACCTACCAGA CTGAGGAGGA CAAGAAGAAC 
ATCAACAGGC TGCAGGATCT GGTTGATAAG CTTCAGCTGA AGGTCAAGGC TTACAAGAGA 
CAGTCTGAAG AAGCTGAGGA ACAAGCCAAC TCTCACCTGT CCAAGTTGAG GAAGGTGCAG 
CATGAGCTGG AGGAGGCTGA GGAGCGCGCT GACATTTCTG AATCCCAGGT CAACAAGCTC 
AGAGCCAAGA GCCGTGACGC TGGAAAGGCT AAGGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myha ( XM_021476518.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332193.1 Danio rerio myosin, heavy chain a (myha), mRNA.

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CloneID	ODa10615
Clone ID Related Accession (Same CDS sequence)	XM_001923974.6 , XM_021476518.1
Accession Version	XM_021476518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	myosin heavy chain, fast skeletal muscle
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAAA GAATTGAGGC TCAGAACAAA CCATTTGATG CCAAATCTGC TTGCTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGAAGA TGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGACTGTTCT GCGCCACTGT AAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA AAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCGGTGACAA GAAGAAAGAG CAGACTCCAG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG TACAACTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAAGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCAACTGA TACTGCTATT 
GACATTCTGG GCTTCACTGG TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCTCTTAC CTTCTGGGTT TGAACTCTGC TGAAATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGTTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCTATGGGAA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AGGCCTCAAA AAGGCAAGGC TGAGGCTCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACG TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTGTACC AGAAGTCTTC TGTCAAACTG 
TTGGCTACTC TCTACCCACC CGTCGTTGAG GACACTTCAA AGAAGGGAGG CAAGAAGAAG 
GGTGGTTCCA TGCAGACTGT GTCTTCTCAG TTCAGGGAGA ACTTGGGCAA GCTAATGACC 
AACTTGAGGA GCACTCACCC TCATTTTGTG CGTTGTCTGA TTCCCAATGA GTCCAAGACT 
CCAGGTCTTA TGGAGAACTT CCTAGTTATC CACCAGCTGA GGTGTAACGG TGTACTGGAG 
GGTATCAGAA TCTGTAGAAA GGGTTTCCCA AGCAGAATTC TTTATGCCGA CTTCAAGCAG 
AGATACAAGG TGTTGAATGC CAGTGTTATC CCAGAGGGAC AGTTCATTGA CAACAAGAAG 
GCCAGTGAGA AACTCCTGGG ATCTATTGAT GTTAATCATG ATGAGTACAG ATTTGGACAC 
ACAAAGGTGT TCTTCAAAGC TGGTCTGCTG GGTACTCTTG AGGAGATGCG TGATGAGAAA 
CTGGCTAGTC TGGTCACAAT GACTCAGGCT CTCTGCCGTG CTTATCTGAT GAGGAGAGAG 
TTTGTGAAGA TGATGGAGAG GAGGGAGTCC ATTTACACCA TCCAGTACAA CATCCGCTCA 
TTCATGAATG TCAAACACTG GCCATGGATG AAGGTTTACT ACAAGATTAA GCCTCTGCTG 
AAGAGTGCTG AGACTGAGAA AGAACTGGCA AACATGAAAG AGGACTTTGT CAAATGCAAA 
GAAGATCTTG TCAAGGCTGA AGCTAAAAAG AAGGAGCTGG AAGAGAAGAT GGTGGCACTG 
CTGCAAGAGA AAAATGATCT GCAGCTGGCA GTGGCTTCTG AAGCTGAAAA TCTCTCAGAT 
GCTGAGGAGA GGTGTGAGGG TCTGATCAAG AGCAAAATCC AGCTTGAGGC TAAACTCAAA 
GAGACAACTG AGAGACTGGA GGATGAGGAA GAAATCAATG CTGAACTGAC AGCCAAGAAG 
AGAAAACTGG AGGACGAGTG CTCTGAGCTG AAGAAAGACA TTGATGACCT GGAGCTCACC 
TTGGCTAAAG TGGAAAAGGA GAAACATGCC ACTGAGAATA AGGTTAAGAA CTTGACAGAG 
GAAATGGCAT CTCAGGATGA GAGCATTGCC AAGCTTACAA AGGAGAAGAA AGCCCTCCAA 
GAGGCACATC AGCAGACTCT GGATGATCTT CAGGCTGAGG AGGACAAAGT CAACACCCTG 
ACCAAATCCA AGACAAAGCT TGAGCAGCAA GTTGATGATC TTGAGGGTTC CCTTGAACAA 
GAAAAGAAGC TCCGTATGGA CCTGGAGAGA GCTAAGAGAA AGCTTGAAGG AGATCTAAAA 
TTGGCCCAAG AGTCTATCAT GGACCTGGAA AATGACAAGC AGCAGTCTGA AGAGAAAATT 
AAAAAGAAAG ACTTTGAAAC AAGCCAGCTG CTCAGCAAGA TTGAAGATGA ACAATCCTTG 
GGTGCTCAGC TCCAGAAAAA GATCAAGGAG CTTCAGGCTC GCATTGAGGA ACTGGAGGAA 
GAGATTGAGG CTGAGCGTGC TGCTCGTGCC AAGGTTGAGA AGCAGAGAGC AGATCTCTCC 
AGGGAACTTG AGGAGATCAG TGAGAGGCTT GAGGAGGCTG GAGGAGCCAC TGCTGCTCAA 
ATCGAGATGA ATAAGAAGCG TGAAGCTGAA TTCCAGAAGC TGCGTCGTGA TCTTGAAGAG 
TCCACCCTTC AGCATGAAGC TACTGCTGCT GCCCTGCGCA AGAAGCAGGC AGACAGTGTG 
GCTGAGCTGG GAGAGCAAAT CGACAACCTC CAGCGTGTCA AGCAGAAGCT TGAGAAAGAG 
AAGAGTGAAT ACAAAATGGA GATCGATGAT CTCTCCAGCA ATATGGAGGC TGTCGCCAAA 
GCAAAGGCCA ATCTTGAGAA GATGTGTCGC ACACTTGAGG ATCAACTTAG TGAAATAAAG 
TCCAAGAGTG ATGAGAACCT TCGCCAGATA AATGATCTCA GTGCTCAAAG AGCAAGACTT 
CAAACTGAGA ATGGTGAGTT TGGCCGTCAG CTGGAGGAGA AAGAAGCTCT GGTTTCTCAG 
CTCACAAGAG GAAAACAGGC TTTCACTCAG CAAATTGAGG AGCTTAAGAG GCAGATTGAA 
GAGGAGGTCA AGGCTAAGAA CGCACTGGCC CATGCTGTAC AATCAGCCCG TCATGACTGC 
GACCTGCTTC GTGAGCAGTT TGAGGAGGAG CAGGAAGCAA AGGCTGAGCT GCAGCGGGGA 
ATGTCAAAGG CAAACAGTGA GGTTGCTCAG TGGAGAACCA AATATGAAAC TGATGCCATC 
CAGCGCACAG AAGAACTTGA AGAATCCAAG AAGAAACTGG CTCAGCGTCT GCAAGAGGCA 
GAGGAACAAA TTGAGGCAGT GAACTCCAAA TGTGCATCTC TGGAGAAGAC CAAACAGAGA 
CTCCAGGGTG AAGTGGAGGA CCTCATGATT GATGTGGAGA GAGCCAATTC TTTGGCTGCC 
AACCTTGACA AAAAGCAGAG AAACTTTGAC AAGGTCCTGG CAGAATGGAA ACAGAAATAT 
GAGGAAGGTC AGGCAGAGCT GGAAGGTGCC CAGAAAGAAG CTCGTTCACT CAGCACTGAG 
CTGTTCAAGA TGAAGAACTC CTATGAGGAG ACTCTGGATC AGCTGGAGAC CCTCAAGAGA 
GAGAACAAGA ATCTGCAGCA GGAGATTTCA GATCTGACAG AGCAGATAGG TGAGACTGGT 
AAGAGCATCC ATGAGCTGGA AAAGGCCAAG AAGACAGTGG AGACTGAGAA GGCAGAGATT 
CAGACCGCCC TAGAAGAGGC TGAAGGCACC CTGGAGCATG AGGAGTCCAA GATTCTTCGT 
GTCCAGCTTG AGCTAAACCA GGTCAAGGGT GAGATTGACA GGAAGCTTGC AGAGAAGGAT 
GAGGAGATAG AGCAGATCAA GAGGAACAGT CAGAGAGTTA CTGAAGCCAT GCAGAGCACT 
CTGGACTCTG AAGTCAGAAG CAGGAATGAT GCTCTCAGAA TCAAGAAGAA GATGGAGGGA 
GACCTTAATG AAATGGAGAT TCAGCTGAGC CATGCCAACC GCCAGGCTGC TGAGGCCCAG 
AAACAGCTCA GGAATGTTCA GTCACAACTC AAGGATGCCC AGCTGCACCT TGATGATGCT 
GTGAGAGCAC AGGAAGACAT GAAGGAGCAG GTGGCCATGG TGGAGCGCAG AAACACTCTG 
ATGCAGTCTG AGATCGAAGA GCTGAGAGCT GCTCTGGAGC AGACAGAGAG AGGACGCAAA 
GTGGCTGAAC AAGAGCTGGT GGATGCCAGT GAGCGTGTTG GACTTTTGCA CTCTCAGAAC 
ACAAGTCTTC TGAACACGAA GAAGAAGCTT GAGTCTGACC TTGTTCAGAT CCAAAGTGAA 
GTTGAAGACA CTGTACAGGA AGCCAGAAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACT 
GATGCTGCAA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGTGC TCACCTTGAG 
AGGATGAAGA AGAATCTGGA GATCACAGTG AAAGACCTGC AGCACCGTCT GGATGAAGCT 
GAGAATCTGG CCATGAAGGG AGGAAAGAAA CAACTCCAGA AACTGGAGTC CAGAGTTCGT 
GAGCTTGAGA GCGAAATCGA TGCAGAGCAG AGACGTGGAG CTGATGCTGT TAAAGGCGTC 
CGCAAATATG AGAGGAGAGT CAAGGAGCTC ACCTACCAGA CTGAGGAGGA CAAGAAGAAC 
ATCAACAGGC TGCAGGATCT GGTTGATAAG CTTCAGCTGA AGGTCAAGGC TTACAAGAGA 
CAGTCTGAAG AAGCTGAGGA ACAAGCCAAC TCTCACCTGT CCAAGTTGAG GAAGGTGCAG 
CATGAGCTGG AGGAGGCTGA GGAGCGCGCT GACATTTCTG AATCCCAGGT CAACAAGCTC 
AGAGCCAAGA GCCGTGACGC TGGAAAGGCT AAGGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332193.1
CDS	9..5810
Translation	MSTDAEMAVYGKAAIYLRKPEKERIEAQNKPFDAKSACYVVDDKELYVKGTIKSRDGGKVTVITLDTKEERVAKEEDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGGDKKKEQTPGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFTGEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKISYLLGLNSAEMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVIRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPRPQKGKAEAHFSLVHYAGTVDYNVSGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEDTSKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRAYLMRREFVKMMERRESIYTIQYNIRSFMNVKHWPWMKVYYKIKPLLKSAETEKELANMKEDFVKCKEDLVKAEAKKKELEEKMVALLQEKNDLQLAVASEAENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMASQDESIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKIKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKSKSDENLRQINDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANSLAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQIGETGKSIHELEKAKKTVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKGEIDRKLAEKDEEIEQIKRNSQRVTEAMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQSQLKDAQLHLDDAVRAQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLESDLVQIQSEVEDTVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEITVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEIDAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNINRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADISESQVNKLRAKSRDAGKAKEE

Target ORF information:

RefSeq Version	XM_021476518.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, heavy chain a (myha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476518.1

ORF Insert Sequence:

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAAA GAATTGAGGC TCAGAACAAA CCATTTGATG CCAAATCTGC TTGCTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGAAGA TGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGACTGTTCT GCGCCACTGT AAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA AAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCGGTGACAA GAAGAAAGAG CAGACTCCAG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG TACAACTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAAGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCAACTGA TACTGCTATT 
GACATTCTGG GCTTCACTGG TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCTCTTAC CTTCTGGGTT TGAACTCTGC TGAAATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGTTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCTATGGGAA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AGGCCTCAAA AAGGCAAGGC TGAGGCTCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACG TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTGTACC AGAAGTCTTC TGTCAAACTG 
TTGGCTACTC TCTACCCACC CGTCGTTGAG GACACTTCAA AGAAGGGAGG CAAGAAGAAG 
GGTGGTTCCA TGCAGACTGT GTCTTCTCAG TTCAGGGAGA ACTTGGGCAA GCTAATGACC 
AACTTGAGGA GCACTCACCC TCATTTTGTG CGTTGTCTGA TTCCCAATGA GTCCAAGACT 
CCAGGTCTTA TGGAGAACTT CCTAGTTATC CACCAGCTGA GGTGTAACGG TGTACTGGAG 
GGTATCAGAA TCTGTAGAAA GGGTTTCCCA AGCAGAATTC TTTATGCCGA CTTCAAGCAG 
AGATACAAGG TGTTGAATGC CAGTGTTATC CCAGAGGGAC AGTTCATTGA CAACAAGAAG 
GCCAGTGAGA AACTCCTGGG ATCTATTGAT GTTAATCATG ATGAGTACAG ATTTGGACAC 
ACAAAGGTGT TCTTCAAAGC TGGTCTGCTG GGTACTCTTG AGGAGATGCG TGATGAGAAA 
CTGGCTAGTC TGGTCACAAT GACTCAGGCT CTCTGCCGTG CTTATCTGAT GAGGAGAGAG 
TTTGTGAAGA TGATGGAGAG GAGGGAGTCC ATTTACACCA TCCAGTACAA CATCCGCTCA 
TTCATGAATG TCAAACACTG GCCATGGATG AAGGTTTACT ACAAGATTAA GCCTCTGCTG 
AAGAGTGCTG AGACTGAGAA AGAACTGGCA AACATGAAAG AGGACTTTGT CAAATGCAAA 
GAAGATCTTG TCAAGGCTGA AGCTAAAAAG AAGGAGCTGG AAGAGAAGAT GGTGGCACTG 
CTGCAAGAGA AAAATGATCT GCAGCTGGCA GTGGCTTCTG AAGCTGAAAA TCTCTCAGAT 
GCTGAGGAGA GGTGTGAGGG TCTGATCAAG AGCAAAATCC AGCTTGAGGC TAAACTCAAA 
GAGACAACTG AGAGACTGGA GGATGAGGAA GAAATCAATG CTGAACTGAC AGCCAAGAAG 
AGAAAACTGG AGGACGAGTG CTCTGAGCTG AAGAAAGACA TTGATGACCT GGAGCTCACC 
TTGGCTAAAG TGGAAAAGGA GAAACATGCC ACTGAGAATA AGGTTAAGAA CTTGACAGAG 
GAAATGGCAT CTCAGGATGA GAGCATTGCC AAGCTTACAA AGGAGAAGAA AGCCCTCCAA 
GAGGCACATC AGCAGACTCT GGATGATCTT CAGGCTGAGG AGGACAAAGT CAACACCCTG 
ACCAAATCCA AGACAAAGCT TGAGCAGCAA GTTGATGATC TTGAGGGTTC CCTTGAACAA 
GAAAAGAAGC TCCGTATGGA CCTGGAGAGA GCTAAGAGAA AGCTTGAAGG AGATCTAAAA 
TTGGCCCAAG AGTCTATCAT GGACCTGGAA AATGACAAGC AGCAGTCTGA AGAGAAAATT 
AAAAAGAAAG ACTTTGAAAC AAGCCAGCTG CTCAGCAAGA TTGAAGATGA ACAATCCTTG 
GGTGCTCAGC TCCAGAAAAA GATCAAGGAG CTTCAGGCTC GCATTGAGGA ACTGGAGGAA 
GAGATTGAGG CTGAGCGTGC TGCTCGTGCC AAGGTTGAGA AGCAGAGAGC AGATCTCTCC 
AGGGAACTTG AGGAGATCAG TGAGAGGCTT GAGGAGGCTG GAGGAGCCAC TGCTGCTCAA 
ATCGAGATGA ATAAGAAGCG TGAAGCTGAA TTCCAGAAGC TGCGTCGTGA TCTTGAAGAG 
TCCACCCTTC AGCATGAAGC TACTGCTGCT GCCCTGCGCA AGAAGCAGGC AGACAGTGTG 
GCTGAGCTGG GAGAGCAAAT CGACAACCTC CAGCGTGTCA AGCAGAAGCT TGAGAAAGAG 
AAGAGTGAAT ACAAAATGGA GATCGATGAT CTCTCCAGCA ATATGGAGGC TGTCGCCAAA 
GCAAAGGCCA ATCTTGAGAA GATGTGTCGC ACACTTGAGG ATCAACTTAG TGAAATAAAG 
TCCAAGAGTG ATGAGAACCT TCGCCAGATA AATGATCTCA GTGCTCAAAG AGCAAGACTT 
CAAACTGAGA ATGGTGAGTT TGGCCGTCAG CTGGAGGAGA AAGAAGCTCT GGTTTCTCAG 
CTCACAAGAG GAAAACAGGC TTTCACTCAG CAAATTGAGG AGCTTAAGAG GCAGATTGAA 
GAGGAGGTCA AGGCTAAGAA CGCACTGGCC CATGCTGTAC AATCAGCCCG TCATGACTGC 
GACCTGCTTC GTGAGCAGTT TGAGGAGGAG CAGGAAGCAA AGGCTGAGCT GCAGCGGGGA 
ATGTCAAAGG CAAACAGTGA GGTTGCTCAG TGGAGAACCA AATATGAAAC TGATGCCATC 
CAGCGCACAG AAGAACTTGA AGAATCCAAG AAGAAACTGG CTCAGCGTCT GCAAGAGGCA 
GAGGAACAAA TTGAGGCAGT GAACTCCAAA TGTGCATCTC TGGAGAAGAC CAAACAGAGA 
CTCCAGGGTG AAGTGGAGGA CCTCATGATT GATGTGGAGA GAGCCAATTC TTTGGCTGCC 
AACCTTGACA AAAAGCAGAG AAACTTTGAC AAGGTCCTGG CAGAATGGAA ACAGAAATAT 
GAGGAAGGTC AGGCAGAGCT GGAAGGTGCC CAGAAAGAAG CTCGTTCACT CAGCACTGAG 
CTGTTCAAGA TGAAGAACTC CTATGAGGAG ACTCTGGATC AGCTGGAGAC CCTCAAGAGA 
GAGAACAAGA ATCTGCAGCA GGAGATTTCA GATCTGACAG AGCAGATAGG TGAGACTGGT 
AAGAGCATCC ATGAGCTGGA AAAGGCCAAG AAGACAGTGG AGACTGAGAA GGCAGAGATT 
CAGACCGCCC TAGAAGAGGC TGAAGGCACC CTGGAGCATG AGGAGTCCAA GATTCTTCGT 
GTCCAGCTTG AGCTAAACCA GGTCAAGGGT GAGATTGACA GGAAGCTTGC AGAGAAGGAT 
GAGGAGATAG AGCAGATCAA GAGGAACAGT CAGAGAGTTA CTGAAGCCAT GCAGAGCACT 
CTGGACTCTG AAGTCAGAAG CAGGAATGAT GCTCTCAGAA TCAAGAAGAA GATGGAGGGA 
GACCTTAATG AAATGGAGAT TCAGCTGAGC CATGCCAACC GCCAGGCTGC TGAGGCCCAG 
AAACAGCTCA GGAATGTTCA GTCACAACTC AAGGATGCCC AGCTGCACCT TGATGATGCT 
GTGAGAGCAC AGGAAGACAT GAAGGAGCAG GTGGCCATGG TGGAGCGCAG AAACACTCTG 
ATGCAGTCTG AGATCGAAGA GCTGAGAGCT GCTCTGGAGC AGACAGAGAG AGGACGCAAA 
GTGGCTGAAC AAGAGCTGGT GGATGCCAGT GAGCGTGTTG GACTTTTGCA CTCTCAGAAC 
ACAAGTCTTC TGAACACGAA GAAGAAGCTT GAGTCTGACC TTGTTCAGAT CCAAAGTGAA 
GTTGAAGACA CTGTACAGGA AGCCAGAAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACT 
GATGCTGCAA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGTGC TCACCTTGAG 
AGGATGAAGA AGAATCTGGA GATCACAGTG AAAGACCTGC AGCACCGTCT GGATGAAGCT 
GAGAATCTGG CCATGAAGGG AGGAAAGAAA CAACTCCAGA AACTGGAGTC CAGAGTTCGT 
GAGCTTGAGA GCGAAATCGA TGCAGAGCAG AGACGTGGAG CTGATGCTGT TAAAGGCGTC 
CGCAAATATG AGAGGAGAGT CAAGGAGCTC ACCTACCAGA CTGAGGAGGA CAAGAAGAAC 
ATCAACAGGC TGCAGGATCT GGTTGATAAG CTTCAGCTGA AGGTCAAGGC TTACAAGAGA 
CAGTCTGAAG AAGCTGAGGA ACAAGCCAAC TCTCACCTGT CCAAGTTGAG GAAGGTGCAG 
CATGAGCTGG AGGAGGCTGA GGAGCGCGCT GACATTTCTG AATCCCAGGT CAACAAGCTC 
AGAGCCAAGA GCCGTGACGC TGGAAAGGCT AAGGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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myha cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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