myhz2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	myhz2
Entrez Gene ID	246275
Full Name	myosin, heavy polypeptide 2, fast muscle specific
Synonyms	sb:cb38
General protein information	Preferred Names myosin, heavy polypeptide 2, fast muscle specific Names myosin, heavy polypeptide 2, fast muscle specific fmyhc2.1 myo2hc2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005171786.3	XP_005171843.1	myosin, heavy polypeptide 2, fast muscle specific isoform X1
NM_152982.2	NP_694514.2	myosin, heavy polypeptide 2, fast muscle specific
XM_005171786.4	XP_005171843.1	myosin, heavy polypeptide 2, fast muscle specific isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH4	XP_523785.2
Macaca mulatta (Rhesus monkey)	MYH4	XP_002800337.1
Canis lupus familiaris (dog)	MYH4	NP_001070262.1
Danio rerio (zebrafish)	myhz2	NP_694514.2
Danio rerio (zebrafish)	myhz1.2	NP_001154918.1
Xenopus tropicalis (tropical clawed frog)	myh3	NP_001006915.1
Danio rerio (zebrafish)	myhb	XP_001339206.5
Homo sapiens (human)	MYH4	NP_060003.2
Rattus norvegicus (Norway rat)	Myh4	NP_062198.1
Danio rerio (zebrafish)	zgc:66156	XP_002666141.3
Danio rerio (zebrafish)	ns:zf-e68	XP_001924014.2
Bos taurus (cattle)	MYH4	XP_005220494.1
Mus musculus (house mouse)	Myh4	NP_034985.2
Danio rerio (zebrafish)	myhc4	NP_001018321.1
Danio rerio (zebrafish)	myhz1.1	NP_001108561.1
Danio rerio (zebrafish)	wu:fd14a01	XP_002667379.3
Danio rerio (zebrafish)	myha	XP_001924009.1
Xenopus tropicalis (tropical clawed frog)	myh7	NP_001135564.1
Xenopus tropicalis (tropical clawed frog)	LOC100492956	XP_002937039.1
Xenopus tropicalis (tropical clawed frog)	LOC100493121	XP_004919657.1

Gene Ontology
Bibliography

Related articles in PubMed

Defective sarcomere assembly in
Cai M, Han L, Liu L, He F, Chu W, Zhang J, Tian Z, Du S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology33(5)6209-6225(2019 May)

Patterns of alternative splicing in response to cold acclimation in fish.
Healy TM, Schulte PM
The Journal of experimental biology222(Pt 5)(2019 Mar)

Genetic Mutations in jamb, jamc, and myomaker Revealed Different Roles on Myoblast Fusion and Muscle Growth.
Si Y, Wen H, Du S
Marine biotechnology (New York, N.Y.)21(1)111-123(2019 Feb)

Knockout of myomaker results in defective myoblast fusion, reduced muscle growth and increased adipocyte infiltration in zebrafish skeletal muscle.
Shi J, Cai M, Si Y, Zhang J, Du S
Human molecular genetics27(20)3542-3554(2018 10)

Duplication of a Single
Garcia de la Serrana D, Wreggelsworth K, Johnston IA
Frontiers in physiology91724(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following myhz2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myhz2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02969	NM_152982.2 Latest version!	Danio rerio myosin, heavy polypeptide 2, fast muscle specific (myhz2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa10414	XM_005171786.4 Latest version!	Danio rerio myosin, heavy polypeptide 2, fast muscle specific (myhz2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa10414	XM_005171786.3	Danio rerio myosin, heavy polypeptide 2, fast muscle specific (myhz2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myhz2 ( NM_152982.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_694514.2 Danio rerio myosin, heavy polypeptide 2, fast muscle specific (myhz2), mRNA.

Price & Availability↑

CloneID	ODa02969
Clone ID Related Accession (Same CDS sequence)	NM_152982.2
Accession Version	NM_152982.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Danio rerio(zebrafish)
Product	myosin, heavy polypeptide 2, fast muscle specific
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071279.1. On Jul 22, 2004 this sequence version replaced NM_152982.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGAGTACTG ACGCGGAGAT GGCCATTTAT GGCAAGGCTG CCATTTTCCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAGCAAA CCATTTGATG CCAAAACCGC TTGTTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAAAGATGG TGGCAAAGTC 
ACTGTTGTTA CACTTGACAC TCAGACGGAG AAAGTTGTTA AGGAGGATGA TGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT AAACCCCTAT AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAATGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCCGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TCCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCGGTGACAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TCCATTTCGG CACATCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATTACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCACTGG TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCGCATAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCCG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGCAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCTCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC TGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGTG GCGGAAAGAA GGGAGGCAAA 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAAAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC TAACGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGGTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGACAAC 
AAGAAGGCCA GTGAGAAACT CTTGGGATCC ATCGACGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CCCTTGAGGA GATGCGTGAT 
GAGAAACTGG CAACTCTAGT CACAATGACC CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TGAAGATGAT GGAGAGGAGG GAGTCCATTT ACACCATCCA GTACAACATC 
CGCTCATTTA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGTAAAGAGG CTTTGGCCAA GGCTGAAGCC AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AAGAGAGGTG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AGGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAAAAA CATGCCACTG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCATCTCA AGATGAGAGC ATTGCCAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTTCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCCAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTTGA GGGTTCCCTT 
GAACAAGAAA AGAAGCTTCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAT 
TTGAAATTGG CCCAAGAGTC TATTATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAAGAG 
AAAATTAAAA AGAAAGACTT CGAAACTGCT CAGCTGCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATT AAAGAGCTGC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCTGAC 
AGTGTGGCTG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATC GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT TGAGAAGATG TGCCGCACAG TTGAGGACCA ACTTAGTGAA 
ATTAAGTCCA AGAATGATGA GAACCTTCGC CAGATAAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAGAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAACGCA CTGGCCCACG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAG TCTAAGAAGA AGCTTGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAATGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA ATTAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG GCCAAGAAGA CAGTGGAGAC TGAGAAGGCA 
GAGATTCAAA CTGCCCTGGA AGAGGCTGAA GGCACCCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAGGGTGAGA TTGACAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GATCAAGAGG AACAGTCAAA GAGTTACTGA AGCCATGCAG 
AGCACTCTGG ACTCTGAGGT CAGAAGCAGG AATGATGCTC TGAGAATCAA AAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCAGCTGAG 
GCCCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAAG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAC 
ACTCTGATGC AGTCTGAGAT TGAGGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGACT GCTGCACTCT 
CAAAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGA CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTCAAGG ACCTGCAGCA CCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCTAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAAGCA GAGCAGAGGC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAGAG AAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAGAATGTCA ACAGACTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCCAA GTTAAGAAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGTGCTGATA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACGCTGGA AAGGCCAAAG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694514.2
CDS	44..5851
Translation	MSTDAEMAIYGKAAIFLRKPEKERIEAQSKPFDAKTACYVVDDKELYVKGTIKSKDGGKVTVVTLDTQTEKVVKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGGDKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFTGEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIAYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVIRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLATLVTMTQALCRAYLMRREFVKMMERRESIYTIQYNIRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEALAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMASQDESIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKIKKKDFETAQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTVEDQLSEIKSKNDENLRQINDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQLGETGKSIHELEKAKKTVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKGEIDRKLAEKDEEMEQIKRNSQRVTEAMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLETDLVQIQSEVEDTVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEVEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDAGKAKEE

Target ORF information:

RefSeq Version	NM_152982.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, heavy polypeptide 2, fast muscle specific (myhz2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152982.2

ORF Insert Sequence:

ATGAGTACTG ACGCGGAGAT GGCCATTTAT GGCAAGGCTG CCATTTTCCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAGCAAA CCATTTGATG CCAAAACCGC TTGTTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAAAGATGG TGGCAAAGTC 
ACTGTTGTTA CACTTGACAC TCAGACGGAG AAAGTTGTTA AGGAGGATGA TGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT AAACCCCTAT AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAATGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCCGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TCCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCGGTGACAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TCCATTTCGG CACATCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATTACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCACTGG TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCGCATAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCCG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGCAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCTCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC TGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGTG GCGGAAAGAA GGGAGGCAAA 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAAAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC TAACGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGGTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGACAAC 
AAGAAGGCCA GTGAGAAACT CTTGGGATCC ATCGACGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CCCTTGAGGA GATGCGTGAT 
GAGAAACTGG CAACTCTAGT CACAATGACC CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TGAAGATGAT GGAGAGGAGG GAGTCCATTT ACACCATCCA GTACAACATC 
CGCTCATTTA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGTAAAGAGG CTTTGGCCAA GGCTGAAGCC AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AAGAGAGGTG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AGGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAAAAA CATGCCACTG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCATCTCA AGATGAGAGC ATTGCCAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTTCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCCAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTTGA GGGTTCCCTT 
GAACAAGAAA AGAAGCTTCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAT 
TTGAAATTGG CCCAAGAGTC TATTATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAAGAG 
AAAATTAAAA AGAAAGACTT CGAAACTGCT CAGCTGCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATT AAAGAGCTGC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCTGAC 
AGTGTGGCTG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATC GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT TGAGAAGATG TGCCGCACAG TTGAGGACCA ACTTAGTGAA 
ATTAAGTCCA AGAATGATGA GAACCTTCGC CAGATAAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAGAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAACGCA CTGGCCCACG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAG TCTAAGAAGA AGCTTGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAATGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA ATTAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG GCCAAGAAGA CAGTGGAGAC TGAGAAGGCA 
GAGATTCAAA CTGCCCTGGA AGAGGCTGAA GGCACCCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAGGGTGAGA TTGACAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GATCAAGAGG AACAGTCAAA GAGTTACTGA AGCCATGCAG 
AGCACTCTGG ACTCTGAGGT CAGAAGCAGG AATGATGCTC TGAGAATCAA AAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCAGCTGAG 
GCCCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAAG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAC 
ACTCTGATGC AGTCTGAGAT TGAGGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGACT GCTGCACTCT 
CAAAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGA CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTCAAGG ACCTGCAGCA CCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCTAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAAGCA GAGCAGAGGC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAGAG AAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAGAATGTCA ACAGACTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCCAA GTTAAGAAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGTGCTGATA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACGCTGGA AAGGCCAAAG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myhz2 ( XM_005171786.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171843.1 Danio rerio myosin, heavy polypeptide 2, fast muscle specific (myhz2), transcript variant X1, mRNA.

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CloneID	ODa10414
Clone ID Related Accession (Same CDS sequence)	XM_005171786.3 , XM_005171786.4
Accession Version	XM_005171786.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	myosin, heavy polypeptide 2, fast muscle specific isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171786.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACTG ACGCGGAGAT GGCCATTTAT GGCAAGGCTG CCATTTTCCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAGCAAA CCATTTGATG CCAAAACCGC TTGTTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAAAGATGG TGGCAAAGTC 
ACTGTTGTTA CACTTGACAC TCAGACGGAG AAAGTTGTTA AGGAGGATGA TGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT AAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TCCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCGGTGACAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACATCTGGA 
AAACTGGCTA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCACTGG TGAAGAGAAA ATGGGTATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCGCTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCCG AGTGAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCTCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC TGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGTG GCGGAAAGAA GGGAGGCAAA 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAAAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC TAACGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGGTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGACAAC 
AAGAAGGCCA GTGAGAAACT CTTGGGATCC ATCGACGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CCCTTGAGGA GATGCGTGAT 
GAGAAACTGG CAACTCTAGT CACAATGACC CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TGAAGATGAT GGAGAGGAGG GAGTCCATTT ACACCATCCA GTACAACATC 
CGCTCATTTA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGTAAAGAGG CTTTGGCCAA GGCTGAAGCC AAAAAGAAAG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AAGAGAGGTG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AGGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAAAAA CATGCCACTG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCATCTCA AGATGAGAGC ATTGCCAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTTCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCCAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTTGA GGGTTCCCTT 
GAACAAGAAA AGAAGCTTCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAT 
TTGAAATTGG CCCAAGAGTC TATTATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAAGAG 
AAAATTAAAA AGAAAGACTT CGAAACTGCT CAGCTGCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATT AAAGAGCTGC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCTGAC 
AGTGTGGCTG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATC GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT TGAGAAGATG TGCCGCACAG TTGAGGACCA ACTTAGTGAA 
ATTAAGTCCA AGAATGATGA GAACCTTCGC CAGATAAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAGAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAACGCA CTGGCCCACG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAG TCTAAGAAGA AGCTTGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAATGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA ATTAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG GCCAAGAAGA CAGTGGAGAC TGAGAAGGCA 
GAGATTCAAA CTGCCCTGGA AGAGGCTGAA GGCACCCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAGGGTGAGA TTGACAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GATCAAGAGG AACAGTCAGA GAGTTACTGA AGCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCAGCTGAG 
GCCCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAAG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAC 
ACTCTGATGC AGTCTGAGAT TGAGGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAAAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGA CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTCAAGG ACCTGCAGCA CCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAAAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAAGCA GAGCAGAGGC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAGAG AAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAGAATGTCA ACAGACTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCCAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGTGCTGATA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACGCTGGA AAGGCCAAAG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171843.1
CDS	103..5910
Translation	MSTDAEMAIYGKAAIFLRKPEKERIEAQSKPFDAKTACYVVDDKELYVKGTIKSKDGGKVTVVTLDTQTEKVVKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGGDKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFTGEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIAYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVIRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLATLVTMTQALCRAYLMRREFVKMMERRESIYTIQYNIRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEALAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMASQDESIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKIKKKDFETAQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTVEDQLSEIKSKNDENLRQINDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQLGETGKSIHELEKAKKTVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKGEIDRKLAEKDEEMEQIKRNSQRVTEAMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLETDLVQIQSEVEDTVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEVEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDAGKAKEE

Target ORF information:

RefSeq Version	XM_005171786.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin, heavy polypeptide 2, fast muscle specific (myhz2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171786.3

ORF Insert Sequence:

ATGAGTACTG ACGCGGAGAT GGCCATTTAT GGCAAGGCTG CCATTTTCCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAGCAAA CCATTTGATG CCAAAACCGC TTGTTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAAAGATGG TGGCAAAGTC 
ACTGTTGTTA CACTTGACAC TCAGACGGAG AAAGTTGTTA AGGAGGATGA TGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT AAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TCCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCGGTGACAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACATCTGGA 
AAACTGGCTA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCACTGG TGAAGAGAAA ATGGGTATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCGCTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCCG AGTGAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCTCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC TGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGTG GCGGAAAGAA GGGAGGCAAA 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAAAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC TAACGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGGTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGACAAC 
AAGAAGGCCA GTGAGAAACT CTTGGGATCC ATCGACGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CCCTTGAGGA GATGCGTGAT 
GAGAAACTGG CAACTCTAGT CACAATGACC CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TGAAGATGAT GGAGAGGAGG GAGTCCATTT ACACCATCCA GTACAACATC 
CGCTCATTTA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGTAAAGAGG CTTTGGCCAA GGCTGAAGCC AAAAAGAAAG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AAGAGAGGTG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AGGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAAAAA CATGCCACTG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCATCTCA AGATGAGAGC ATTGCCAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTTCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCCAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTTGA GGGTTCCCTT 
GAACAAGAAA AGAAGCTTCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAT 
TTGAAATTGG CCCAAGAGTC TATTATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAAGAG 
AAAATTAAAA AGAAAGACTT CGAAACTGCT CAGCTGCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATT AAAGAGCTGC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCTGAC 
AGTGTGGCTG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATC GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT TGAGAAGATG TGCCGCACAG TTGAGGACCA ACTTAGTGAA 
ATTAAGTCCA AGAATGATGA GAACCTTCGC CAGATAAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAGAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAACGCA CTGGCCCACG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAG TCTAAGAAGA AGCTTGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAATGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA ATTAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG GCCAAGAAGA CAGTGGAGAC TGAGAAGGCA 
GAGATTCAAA CTGCCCTGGA AGAGGCTGAA GGCACCCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAGGGTGAGA TTGACAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GATCAAGAGG AACAGTCAGA GAGTTACTGA AGCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCAGCTGAG 
GCCCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAAG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAC 
ACTCTGATGC AGTCTGAGAT TGAGGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAAAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGA CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTCAAGG ACCTGCAGCA CCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAAAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAAGCA GAGCAGAGGC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAGAG AAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAGAATGTCA ACAGACTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCCAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGTGCTGATA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACGCTGGA AAGGCCAAAG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myhz2 ( XM_005171786.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171843.1 Danio rerio myosin, heavy polypeptide 2, fast muscle specific (myhz2), transcript variant X1, mRNA.

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CloneID	ODa10414
Clone ID Related Accession (Same CDS sequence)	XM_005171786.3 , XM_005171786.4
Accession Version	XM_005171786.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	myosin, heavy polypeptide 2, fast muscle specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171786.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACTG ACGCGGAGAT GGCCATTTAT GGCAAGGCTG CCATTTTCCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAGCAAA CCATTTGATG CCAAAACCGC TTGTTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAAAGATGG TGGCAAAGTC 
ACTGTTGTTA CACTTGACAC TCAGACGGAG AAAGTTGTTA AGGAGGATGA TGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT AAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TCCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCGGTGACAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACATCTGGA 
AAACTGGCTA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCACTGG TGAAGAGAAA ATGGGTATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCGCTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCCG AGTGAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCTCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC TGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGTG GCGGAAAGAA GGGAGGCAAA 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAAAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC TAACGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGGTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGACAAC 
AAGAAGGCCA GTGAGAAACT CTTGGGATCC ATCGACGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CCCTTGAGGA GATGCGTGAT 
GAGAAACTGG CAACTCTAGT CACAATGACC CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TGAAGATGAT GGAGAGGAGG GAGTCCATTT ACACCATCCA GTACAACATC 
CGCTCATTTA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGTAAAGAGG CTTTGGCCAA GGCTGAAGCC AAAAAGAAAG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AAGAGAGGTG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AGGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAAAAA CATGCCACTG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCATCTCA AGATGAGAGC ATTGCCAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTTCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCCAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTTGA GGGTTCCCTT 
GAACAAGAAA AGAAGCTTCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAT 
TTGAAATTGG CCCAAGAGTC TATTATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAAGAG 
AAAATTAAAA AGAAAGACTT CGAAACTGCT CAGCTGCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATT AAAGAGCTGC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCTGAC 
AGTGTGGCTG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATC GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT TGAGAAGATG TGCCGCACAG TTGAGGACCA ACTTAGTGAA 
ATTAAGTCCA AGAATGATGA GAACCTTCGC CAGATAAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAGAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAACGCA CTGGCCCACG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAG TCTAAGAAGA AGCTTGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAATGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA ATTAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG GCCAAGAAGA CAGTGGAGAC TGAGAAGGCA 
GAGATTCAAA CTGCCCTGGA AGAGGCTGAA GGCACCCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAGGGTGAGA TTGACAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GATCAAGAGG AACAGTCAGA GAGTTACTGA AGCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCAGCTGAG 
GCCCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAAG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAC 
ACTCTGATGC AGTCTGAGAT TGAGGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAAAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGA CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTCAAGG ACCTGCAGCA CCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAAAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAAGCA GAGCAGAGGC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAGAG AAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAGAATGTCA ACAGACTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCCAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGTGCTGATA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACGCTGGA AAGGCCAAAG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171843.1
CDS	150..5957
Translation	MSTDAEMAIYGKAAIFLRKPEKERIEAQSKPFDAKTACYVVDDKELYVKGTIKSKDGGKVTVVTLDTQTEKVVKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGGDKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFTGEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIAYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVIRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLATLVTMTQALCRAYLMRREFVKMMERRESIYTIQYNIRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEALAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMASQDESIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKIKKKDFETAQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTVEDQLSEIKSKNDENLRQINDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQLGETGKSIHELEKAKKTVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKGEIDRKLAEKDEEMEQIKRNSQRVTEAMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLETDLVQIQSEVEDTVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEVEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDAGKAKEE

Target ORF information:

RefSeq Version	XM_005171786.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, heavy polypeptide 2, fast muscle specific (myhz2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171786.4

ORF Insert Sequence:

ATGAGTACTG ACGCGGAGAT GGCCATTTAT GGCAAGGCTG CCATTTTCCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAGCAAA CCATTTGATG CCAAAACCGC TTGTTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAAAGATGG TGGCAAAGTC 
ACTGTTGTTA CACTTGACAC TCAGACGGAG AAAGTTGTTA AGGAGGATGA TGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT AAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TCCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCGGTGACAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACATCTGGA 
AAACTGGCTA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCACTGG TGAAGAGAAA ATGGGTATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCGCTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCCG AGTGAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCTCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC TGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGTG GCGGAAAGAA GGGAGGCAAA 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAAAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC TAACGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGGTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGACAAC 
AAGAAGGCCA GTGAGAAACT CTTGGGATCC ATCGACGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CCCTTGAGGA GATGCGTGAT 
GAGAAACTGG CAACTCTAGT CACAATGACC CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TGAAGATGAT GGAGAGGAGG GAGTCCATTT ACACCATCCA GTACAACATC 
CGCTCATTTA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGTAAAGAGG CTTTGGCCAA GGCTGAAGCC AAAAAGAAAG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AAGAGAGGTG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AGGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAAAAA CATGCCACTG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCATCTCA AGATGAGAGC ATTGCCAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTTCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCCAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTTGA GGGTTCCCTT 
GAACAAGAAA AGAAGCTTCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAT 
TTGAAATTGG CCCAAGAGTC TATTATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAAGAG 
AAAATTAAAA AGAAAGACTT CGAAACTGCT CAGCTGCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATT AAAGAGCTGC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCTGAC 
AGTGTGGCTG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATC GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT TGAGAAGATG TGCCGCACAG TTGAGGACCA ACTTAGTGAA 
ATTAAGTCCA AGAATGATGA GAACCTTCGC CAGATAAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAGAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAACGCA CTGGCCCACG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAG TCTAAGAAGA AGCTTGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAATGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA ATTAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG GCCAAGAAGA CAGTGGAGAC TGAGAAGGCA 
GAGATTCAAA CTGCCCTGGA AGAGGCTGAA GGCACCCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAGGGTGAGA TTGACAGGAA GCTGGCAGAG 
AAGGATGAGG AGATGGAGCA GATCAAGAGG AACAGTCAGA GAGTTACTGA AGCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCAGCTGAG 
GCCCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAAG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAC 
ACTCTGATGC AGTCTGAGAT TGAGGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAAAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGA CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTCAAGG ACCTGCAGCA CCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAAAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAAGCA GAGCAGAGGC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAGAG AAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAGAATGTCA ACAGACTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCCAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGTGCTGATA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACGCTGGA AAGGCCAAAG AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defective sarcomere assembly in
FASEB journal : official publication of the Federation of American Societies for Experimental Biology33(5)6209-6225(2019 May)
Cai M,Han L,Liu L,He F,Chu W,Zhang J,Tian Z,Du S

Patterns of alternative splicing in response to cold acclimation in fish.
The Journal of experimental biology222(Pt 5)(2019 Mar)
Healy TM,Schulte PM

Genetic Mutations in jamb, jamc, and myomaker Revealed Different Roles on Myoblast Fusion and Muscle Growth.
Marine biotechnology (New York, N.Y.)21(1)111-123(2019 Feb)
Si Y,Wen H,Du S

Knockout of myomaker results in defective myoblast fusion, reduced muscle growth and increased adipocyte infiltration in zebrafish skeletal muscle.
Human molecular genetics27(20)3542-3554(2018 10)
Shi J,Cai M,Si Y,Zhang J,Du S

Duplication of a Single
Frontiers in physiology91724(2018)
Garcia de la Serrana D,Wreggelsworth K,Johnston IA

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myhz2 cDNA ORF clone, Danio rerio(zebrafish)

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