ns:zf-e68 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ns:zf-e68
Entrez Gene ID	100008070
Full Name	ns:zf-e68
Synonyms	myhz1
General protein information	Preferred Names ns:zf-e68 Names myosin heavy chain, fast skeletal muscle fast skeletal muscle myosin heavy polypeptide 1 myosin, heavy polypeptide 2, fast muscle
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001923979.6	XP_001924014.2	myosin heavy chain, fast skeletal muscle
XM_001923979.7	XP_001924014.2	myosin heavy chain, fast skeletal muscle

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH4	XP_523785.2
Macaca mulatta (Rhesus monkey)	MYH4	XP_002800337.1
Canis lupus familiaris (dog)	MYH4	NP_001070262.1
Danio rerio (zebrafish)	myhz2	NP_694514.2
Danio rerio (zebrafish)	myhz1.2	NP_001154918.1
Xenopus tropicalis (tropical clawed frog)	myh3	NP_001006915.1
Danio rerio (zebrafish)	myhb	XP_001339206.5
Homo sapiens (human)	MYH4	NP_060003.2
Rattus norvegicus (Norway rat)	Myh4	NP_062198.1
Danio rerio (zebrafish)	zgc:66156	XP_002666141.3
Danio rerio (zebrafish)	ns:zf-e68	XP_001924014.2
Bos taurus (cattle)	MYH4	XP_005220494.1
Mus musculus (house mouse)	Myh4	NP_034985.2
Danio rerio (zebrafish)	myhc4	NP_001018321.1
Danio rerio (zebrafish)	myhz1.1	NP_001108561.1
Danio rerio (zebrafish)	wu:fd14a01	XP_002667379.3
Danio rerio (zebrafish)	myha	XP_001924009.1
Xenopus tropicalis (tropical clawed frog)	myh7	NP_001135564.1
Xenopus tropicalis (tropical clawed frog)	LOC100492956	XP_002937039.1
Xenopus tropicalis (tropical clawed frog)	LOC100493121	XP_004919657.1

Gene Ontology

The following ns:zf-e68 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ns:zf-e68 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10616	XM_001923979.7 Latest version!	Danio rerio ns:zf-e68 (ns:zf-e68), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa10616	XM_001923979.6	Danio rerio ns:zf-e68 (ns:zf-e68), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ns:zf-e68 ( XM_001923979.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001924014.2 Danio rerio ns:zf-e68 (ns:zf-e68), mRNA.

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CloneID	ODa10616
Clone ID Related Accession (Same CDS sequence)	XM_001923979.6 , XM_001923979.7
Accession Version	XM_001923979.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	myosin heavy chain, fast skeletal muscle
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001923979.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACTG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAGGC TCAAAACAAA CCATTTGATG CCAAAACCGC TTGTTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGAAAAGTC 
ACTGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAATGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACTGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTA 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TCCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATC 
GACATTCTGG GCTTCAATGC TGAAGAGAAA ATGGGCATCT ACAAGTTTAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCGCATAC CTTCTGGGTT TGAACTCTGC TGATATGCTT 
AAGGCTTTGT GTTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC TGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GCGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAATTT AGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGGTGACTTC 
AAGCAGAGAT ACAAGGTGCT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGACAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TGAAGATGAT GGAGAGGAGG GAGTCCATTT ACACCATCCA ATACAACATT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTATTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAGG CTTTGGCCAA GGCTGAAGCC AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAGAGGTG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCCAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAT 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAG 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATT AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCTG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA AAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATC GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCTAACCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCCA AGAATGATGA GAACATTCGC CAGATAAATG ATCTCAGTGC CCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTGG AGGAAAAAGA GGCTCTGGTT 
TCTCAGCTCA CAAGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTTCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGTATGT CAAAGGCCAA CAGCGAGGTT GCCCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AACTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAATGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GTTAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAGAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CTGCCCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAGAGTGAGA TTGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAGTCACTGA ATCTATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TGAGAATCAA GAAGAAGATG 
GAGGGAGATC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCCCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAC 
ACTCTGATGC AGTCTGAGAT TGAGGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GTTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACTGACG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGATC ACAGTCAAGG ACCTGCAGCA CCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGACTGA AATTGAGGCA GAGCAAAGGC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAGAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAGAATGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAAGAACAA GCCAACTCTC ACCTGTCCAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGTGCTGATA TAGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACGCTGGA AAGGGCAAGG AGTCTGCTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001924014.2
CDS	96..5909
Translation	MSTDAEMAVYGKAAIYLRKPEKERIEAQNKPFDAKTACYVVDDKELYVKGTIKSRDGGKVTVITLDTKEERVAKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGPEKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFNAEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIAYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVIRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRAYLMRREFVKMMERRESIYTIQYNIRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEALAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAAQDESIGKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKLKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKSKNDENIRQINDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQLGETGKSIHELEKSKKAVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKSEIDRKLAEKDEEIEQIKRNSQRVTESMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLEADLVQIQSEVEDTVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEITVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELETEIEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDAGKGKESAE

Target ORF information:

RefSeq Version	XM_001923979.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ns:zf-e68 (ns:zf-e68), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001923979.6

ORF Insert Sequence:

ATGAGTACTG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAGGC TCAAAACAAA CCATTTGATG CCAAAACCGC TTGTTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGAAAAGTC 
ACTGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAATGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACTGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTA 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TCCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATC 
GACATTCTGG GCTTCAATGC TGAAGAGAAA ATGGGCATCT ACAAGTTTAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCGCATAC CTTCTGGGTT TGAACTCTGC TGATATGCTT 
AAGGCTTTGT GTTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC TGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GCGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAATTT AGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGGTGACTTC 
AAGCAGAGAT ACAAGGTGCT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGACAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TGAAGATGAT GGAGAGGAGG GAGTCCATTT ACACCATCCA ATACAACATT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTATTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAGG CTTTGGCCAA GGCTGAAGCC AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAGAGGTG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCCAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAT 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAG 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATT AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCTG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA AAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATC GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCTAACCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCCA AGAATGATGA GAACATTCGC CAGATAAATG ATCTCAGTGC CCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTGG AGGAAAAAGA GGCTCTGGTT 
TCTCAGCTCA CAAGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTTCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGTATGT CAAAGGCCAA CAGCGAGGTT GCCCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AACTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAATGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GTTAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAGAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CTGCCCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAGAGTGAGA TTGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAGTCACTGA ATCTATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TGAGAATCAA GAAGAAGATG 
GAGGGAGATC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCCCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAC 
ACTCTGATGC AGTCTGAGAT TGAGGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GTTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACTGACG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGATC ACAGTCAAGG ACCTGCAGCA CCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGACTGA AATTGAGGCA GAGCAAAGGC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAGAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAGAATGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAAGAACAA GCCAACTCTC ACCTGTCCAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGTGCTGATA TAGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACGCTGGA AAGGGCAAGG AGTCTGCTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ns:zf-e68 ( XM_001923979.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001924014.2 Danio rerio ns:zf-e68 (ns:zf-e68), mRNA.

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CloneID	ODa10616
Clone ID Related Accession (Same CDS sequence)	XM_001923979.6 , XM_001923979.7
Accession Version	XM_001923979.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	myosin heavy chain, fast skeletal muscle
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001923979.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACTG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAGGC TCAAAACAAA CCATTTGATG CCAAAACCGC TTGTTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGAAAAGTC 
ACTGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAATGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACTGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTA 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TCCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATC 
GACATTCTGG GCTTCAATGC TGAAGAGAAA ATGGGCATCT ACAAGTTTAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCGCATAC CTTCTGGGTT TGAACTCTGC TGATATGCTT 
AAGGCTTTGT GTTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC TGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GCGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAATTT AGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGGTGACTTC 
AAGCAGAGAT ACAAGGTGCT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGACAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TGAAGATGAT GGAGAGGAGG GAGTCCATTT ACACCATCCA ATACAACATT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTATTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAGG CTTTGGCCAA GGCTGAAGCC AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAGAGGTG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCCAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAT 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAG 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATT AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCTG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA AAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATC GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCTAACCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCCA AGAATGATGA GAACATTCGC CAGATAAATG ATCTCAGTGC CCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTGG AGGAAAAAGA GGCTCTGGTT 
TCTCAGCTCA CAAGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTTCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGTATGT CAAAGGCCAA CAGCGAGGTT GCCCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AACTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAATGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GTTAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAGAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CTGCCCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAGAGTGAGA TTGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAGTCACTGA ATCTATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TGAGAATCAA GAAGAAGATG 
GAGGGAGATC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCCCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAC 
ACTCTGATGC AGTCTGAGAT TGAGGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GTTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACTGACG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGATC ACAGTCAAGG ACCTGCAGCA CCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGACTGA AATTGAGGCA GAGCAAAGGC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAGAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAGAATGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAAGAACAA GCCAACTCTC ACCTGTCCAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGTGCTGATA TAGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACGCTGGA AAGGGCAAGG AGTCTGCTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001924014.2
CDS	121..5934
Translation	MSTDAEMAVYGKAAIYLRKPEKERIEAQNKPFDAKTACYVVDDKELYVKGTIKSRDGGKVTVITLDTKEERVAKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGPEKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFNAEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIAYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVIRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRAYLMRREFVKMMERRESIYTIQYNIRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEALAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAAQDESIGKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKLKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKSKNDENIRQINDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQLGETGKSIHELEKSKKAVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKSEIDRKLAEKDEEIEQIKRNSQRVTESMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLEADLVQIQSEVEDTVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEITVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELETEIEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDAGKGKESAE

Target ORF information:

RefSeq Version	XM_001923979.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ns:zf-e68 (ns:zf-e68), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001923979.7

ORF Insert Sequence:

ATGAGTACTG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAGGC TCAAAACAAA CCATTTGATG CCAAAACCGC TTGTTATGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGAAAAGTC 
ACTGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCACCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAATGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACTGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTA 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TCCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATC 
GACATTCTGG GCTTCAATGC TGAAGAGAAA ATGGGCATCT ACAAGTTTAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATCGCATAC CTTCTGGGTT TGAACTCTGC TGATATGCTT 
AAGGCTTTGT GTTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTGCCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCATTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC TGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GCGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAATTT AGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGGTGACTTC 
AAGCAGAGAT ACAAGGTGCT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGACAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TGAAGATGAT GGAGAGGAGG GAGTCCATTT ACACCATCCA ATACAACATT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTATTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAGG CTTTGGCCAA GGCTGAAGCC AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAGAGGTG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCCAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAT 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAG 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATT AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCTG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA AAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATC GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCTAACCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCCA AGAATGATGA GAACATTCGC CAGATAAATG ATCTCAGTGC CCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTGG AGGAAAAAGA GGCTCTGGTT 
TCTCAGCTCA CAAGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTTCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGTATGT CAAAGGCCAA CAGCGAGGTT GCCCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AACTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAATGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GTTAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAGAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CTGCCCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAGAGTGAGA TTGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAGTCACTGA ATCTATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TGAGAATCAA GAAGAAGATG 
GAGGGAGATC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCCCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAC 
ACTCTGATGC AGTCTGAGAT TGAGGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GTTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGAGAAGGC CAAGAAGGCC 
ATCACTGACG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGATC ACAGTCAAGG ACCTGCAGCA CCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGACTGA AATTGAGGCA GAGCAAAGGC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAGAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAGAATGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAAGAACAA GCCAACTCTC ACCTGTCCAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGTGCTGATA TAGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACGCTGGA AAGGGCAAGG AGTCTGCTGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ns:zf-e68 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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