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An empirical finding that in DNA the frequency of A equals the frequency of T, and the frequency of G equals the frequency of C; later given a theoretical basis by the Watson-Crick double-helix model of DNA.
A method for prediction of the secondary structure of a protein from its primary structure. A survey of proteins of known secondary and tertiary structure allows an empirical classification of amino acid residues according to their abilities to strengthen or destabilize α-helix or α-helices, then use empirically assigned interaction coefficients to locate the helices in spatial relation to each other.Skolnick, J., Kolinski, A., Brooks, C.L., III, et al. (1993) Curr. Biol. 3, 414-423 Learn more about amino acid chart.
(see solvent-accessible surface)
A microsatellite sequence, a repeated CG sequence in genomic DNA, sometimes the sites of methylations.
The decrease in cellular respiration caused by increased glucose concentrations. (see also Pasteur effect)
A designation of one arbitrarily chosen strand of double-stranded DNA to distinguish it from the other, called the Watson strand.
complementary DNA; DNA that is synthesized, by reverse transcriptase, from an mRNA template, and therefore has no introns. (see also genomic DNA)
A collection of cells, usually E. coli, transformed by DNA vectors each of which contains a different cDNA insert synthesized from a collection of mRNA species. (see also genomic library)
In laboratory animal nutrition, feeding a diet of low nutritional value, i.e. high in calories but low in protein and vitamins.
A mechanism of cellular metabolic control: inositol 1,4,5-trisphosphate, generated in response to cell stimulation, causes a flow of Ca2+ across the plasma membrane, which in turn leads to periodic release of Ca2+ from the endoplasmic reticulum by an inositol trisphosphate-independent mechanism. Berridge, M.J. (1990) J. Biol. Chem. 265, 9583-9586
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