nrde2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nrde2
Entrez Gene ID	780328
Full Name	NRDE-2, necessary for RNA interference, domain containing
Synonyms	c14orf102
General protein information	Preferred Names NRDE-2, necessary for RNA interference, domain containing Names protein NRDE2 homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031890600.1	XP_031746460.1	nuclear exosome regulator NRDE2 isoform X1
NM_001079399.1	NP_001072867.1	nuclear exosome regulator NRDE2
XM_031890601.1	XP_031746461.1	nuclear exosome regulator NRDE2 isoform X2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	NRDE2	XP_001090586.1
Rattus norvegicus (Norway rat)	Nrde2	XP_234465.5
Arabidopsis thaliana (thale cress)	AT3G17740	NP_188401.1
Homo sapiens (human)	NRDE2	NP_060440.2
Gallus gallus (chicken)	C5H14ORF102	XP_421314.3
Arabidopsis thaliana (thale cress)	AT3G17712	NP_001154620.1
Oryza sativa (rice)	Os02g0772500	NP_001048265.1
Mus musculus (house mouse)	Nrde2	NP_898978.2
Xenopus tropicalis (tropical clawed frog)	nrde2	NP_001072867.1
Canis lupus familiaris (dog)	NRDE2	XP_547952.3
Bos taurus (cattle)	NRDE2	NP_001193436.1
Pan troglodytes (chimpanzee)	NRDE2	XP_001141630.1
Danio rerio (zebrafish)	LOC794348	XP_005158613.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nrde2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nrde2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa25178	NM_001079399.1 Latest version!	Xenopus tropicalis NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa63830	XM_031890600.1 Latest version!	Xenopus tropicalis NRDE-2, necessary for RNA interference, domain containing (nrde2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa63831	XM_031890601.1 Latest version!	Xenopus tropicalis NRDE-2, necessary for RNA interference, domain containing (nrde2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nrde2 ( NM_001079399.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072867.1 Xenopus tropicalis NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.

Price & Availability↑

CloneID	OXa25178
Clone ID Related Accession (Same CDS sequence)	NM_001079399.1
Accession Version	NM_001079399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576944000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear exosome regulator NRDE2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC123943.1. ##Evidence-Data-START## Transcript exon combination :: BC123943.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCTAT TTCCAGCCTT TGCAGACTCT TCTAGTTCTA GTAAGAAATC TCCTTCACGC 
CAAGAGTTGG ACTGGCTGAG CAACAAGAGC TTCTGCACAG AAGACGCTCT TTCACTACAC 
CAGCGTGCTT TGCAGTCTGC ACAATTTCCT CAACCTGCAT GCCACTCACC CAGGAGTCCA 
TTTCAGCAAG AGCTGTTTGA AGAAAGTGGA TCTGATTCTC AGGCAACAAA GAAGAAGAAG 
AAAAAGAAAA AAAAAAAGAA ACAGAAAAAA AAGCACAAGC AAAAGAGGGG TTATGATGAG 
GATGAAGAAG GGAATACAAG TGACTCTGAC TCAAGGCCTG AGACAAGGGA ATCTAAAGAG 
ACTGAAAGTG TGGCAAGGCA GGACACTTGT GTTGTGCTCC CTGGCCGTGG TATCTGGCTA 
GATGAAGTGC AGTCTGTGAC AGAGGAGACA TATCGAATTG ATAAGAAATC TGACCCTGCC 
AACTGGGAAT ATAAGTCACT GTACCAAGGA GACATAGCAC GGTACAAACG GAAAGGAAAT 
TCCTGTCTGG GCATCAATCC AAAGAAGCAG CAAATTAATT GGCAGGACGC ATTATCTGAG 
AAGAAAGAAG CACGCAGGAA AAATGAGCGT TATTTTACCA AGAGCACAAT GCAGCTTCTC 
AGTGCCAATG CAGATTTTGT GTCTTGTAAG GAAAGTGCAA GTCTGAAATC AGGGGAATTT 
ATTCCTGTGT TAGATCCTGA TAAGGATGGT GTTATTTCCT CCAGTGGCCC TATTAGCTGG 
GTGAATCCTT TGGGAGTGTA TGATTCTTCT ACAGCTCTGT GGTTGGAAGG AAAAGGGGGT 
CCAGAATATG ATACTATACA GCCAGTACCC CGGGACAACA ATATTTTGGC AAAGGTGGAG 
GATTATAACA AGCGGATCAG AGAATGCCCT AACGATATCC AACTATGGAT GGAGTTTGTC 
TCTTTTCAGG ATGAGTTGGT GAAGCAGCCC AGCATGTATT CTACCAGCAA AGGAGAGCTT 
GATGGCCATC GGACATCTGT GAAGCTGCTA CTGGAGAAAA AACTAGCTAT ACTAGAAAGA 
GCCATTGAGA GTAATCCGGG CAGCACTGAA TTAAAGCTTG CAAAGCTAAA GCTCTGTGAG 
GAATTCTGGG AGGCACCCAC TCTTCTCAAG GAATGGCAGA AACTGATCTT CTTGCACCCA 
AATGACTCGC AGCTGTGGCA GAAATATCTG CTCTTCTGCC AGGGTCAGTT TAGCACTTTC 
TCTGTGTCCA AGATGAACAG TATTTATGGC AAATGCCTAA GCACACTGGC AGCAGTTCAG 
GATGGTATTA TGCTTTCCCA CAATGCTCTG CCAGACACAG AGAGCAACAT GTATGATATT 
TTTCTGCAAC AGTGCCAGTT CTTACGCCAG GCTGGACACA CAGAGAAAGT AATATCCTTA 
TTCCAAGCTC TTACTGACTT CACATTTTAT AAACCGGACA GTGTTAAGGA TATGACAACC 
AAAGAGCAGG TTGACTTTTT TGAGCCTTTT TGGGACAGTG GGGAACCTCG TTTTGGTGAG 
AAAGGAGCAA AGGGATGGAG CTCCTGGATG CGCCAACAGG AGAGAGGGGG CTGGATCATC 
ATTAACAATT TGGTGGAGGA TGATGAAGAC GAAGCAGATG AAGAGTTAAG CATAAGAGGC 
AAAAACTGTC CCAGATACAA GATTTGGTTG GATGTTGAAT GCTCAAGGGA AGCAAGGCAC 
TGGTTGCCAT GGCGTCCAGA CACCACAAAA AAGCAGACAG AGGATGAATG TGAGGATCCA 
GAGAGGCAGG TTCTTTTTGA TGATATTGGC CCGTCAATGT TCAAGATCAC CAGTCCTCAA 
ATGCAGTTCC AGCTCTTTAA ATCATTCCTG CATTTTCTTG GGGTCCCCTG TGGGGCTAAC 
ATTTCCCCAC GCTGCTTTTA CCTTGCTCTT GATGAGACCT CCATCTTTGA TCATGTTTCT 
CCTTATGAAA TGCTGCTTAA CTCGTTTGAA ATCCCTCTGT CTGGAATCGG TACAGTTGGT 
CATTTAGACA CATTAAGCAG AAGCAGGCAA CAAATAGGAT ACTTTAAAGA AGGGGAAACC 
TTCATACAAA ATATCTTTCA GTCAGCTCTG TCACTTTTAC ATGGAGAAGA GAAGAGGAAC 
ATCTCAGTTT GGTGGCTTCA GTATGAAATT TCAAAGGTTG TTTGGTGCCT GCAAGCAAAT 
AACAAGAAAA AGCTGAGATC TCAGGGCAAG AGGAGCAAGC GATTGGCAAA GAGCCTACTG 
AAGGAAGCAT CAAACAGAAA CAGTCTGGCA CTCTGGAAGG AATATGCTCT TCTAGAATGG 
CTTCTGGGCA ATATTGATGA TGCCCGAAAG GTGTTTGATA CAGCCATAAG CTTAGCAGGA 
AGCAAAGGGT TAAAGGACCA GGAATTATGC AGTTTGTGTC TGCTGTATTC AGAACTGGAA 
GGGAAGCTAC TGGACAATCC AGAGGAAAAT GCGGGGTCCC GTGCTGTTCA TATACTTACC 
AGCCTTACAG AAAGCTCTCC TTACACTCCC TATAATAGCC CTGAGCATGC TATTAGTATT 
CTGAAAGCAC GCAAAGTATA TGAACACGCC ATGCAGGACT GCCTGAGGCA ACAGCCACAT 
TGGGGGCGTG TTAGCTTATC TGGATGCTTT GCACTTTTTC AGTATCTAAC TGTGAGCGTT 
GATGCTGCTG TTGTGGTATT AAGACAGGCT GCTGACATCC CAATGACTCC TGAAACATTG 
GACAATGTTG CTCAGAACGC CAGCAGCCCA TTGCAAGCAA TTACACTGAT GCACACAAAT 
CTGCTCAGAC ACCATTATAA GGTCAGTGTC TATCCTCTGA GTCCACTTAG AGACATACTG 
ATGTCTGCCT TAAAGACATA TCCCTGCAAT GTTTCTCTGT GGAAATCCTA CATCAGAACT 
GAAGGTAAAT TACACAATGC CAGCAGGGTG AGAAGGTTTA TAGACAGTAT CAGGAGAATG 
ACACCAGCTC TGGAACCATG TCTGTTCGCC ATCAGAGCAG AGGAGGACAG AAAAAAAATA 
GTAGAATCTG TACAAAGGCC AGATCAAGGG GAGGTTCACT CTATTTTCCC TGAAACTGGT 
TTGTCCAATC GAATCAAGGC CCTTTTTGAA TATGCTCTTC AAAGTGAATA TGGTTCCAGG 
TGCCCTCTGC TCTGGAGGCT GTACTTGCAC TTTATGGTGT CTTTGGGAAA CAAGGAGAGA 
AGCAAAGGCC TGTTCTACAA AGCAATACAG AGCTGCCCAT GGGCAAAGGC ACTATACTTG 
GATGCTGTAG AATATTTCCC GGAGCAGCTG CAAGAGACCA TTGATCTAAT GACTGAAAAG 
GAGCTGAGAG TACGAGTTCC CCTAGAGGAA CTGGATCTGC TGCTGGAAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072867.1
CDS	53..3466
Translation	MALFPAFADSSSSSKKSPSRQELDWLSNKSFCTEDALSLHQRALQSAQFPQPACHSPRSPFQQELFEESGSDSQATKKKKKKKKKKKQKKKHKQKRGYDEDEEGNTSDSDSRPETRESKETESVARQDTCVVLPGRGIWLDEVQSVTEETYRIDKKSDPANWEYKSLYQGDIARYKRKGNSCLGINPKKQQINWQDALSEKKEARRKNERYFTKSTMQLLSANADFVSCKESASLKSGEFIPVLDPDKDGVISSSGPISWVNPLGVYDSSTALWLEGKGGPEYDTIQPVPRDNNILAKVEDYNKRIRECPNDIQLWMEFVSFQDELVKQPSMYSTSKGELDGHRTSVKLLLEKKLAILERAIESNPGSTELKLAKLKLCEEFWEAPTLLKEWQKLIFLHPNDSQLWQKYLLFCQGQFSTFSVSKMNSIYGKCLSTLAAVQDGIMLSHNALPDTESNMYDIFLQQCQFLRQAGHTEKVISLFQALTDFTFYKPDSVKDMTTKEQVDFFEPFWDSGEPRFGEKGAKGWSSWMRQQERGGWIIINNLVEDDEDEADEELSIRGKNCPRYKIWLDVECSREARHWLPWRPDTTKKQTEDECEDPERQVLFDDIGPSMFKITSPQMQFQLFKSFLHFLGVPCGANISPRCFYLALDETSIFDHVSPYEMLLNSFEIPLSGIGTVGHLDTLSRSRQQIGYFKEGETFIQNIFQSALSLLHGEEKRNISVWWLQYEISKVVWCLQANNKKKLRSQGKRSKRLAKSLLKEASNRNSLALWKEYALLEWLLGNIDDARKVFDTAISLAGSKGLKDQELCSLCLLYSELEGKLLDNPEENAGSRAVHILTSLTESSPYTPYNSPEHAISILKARKVYEHAMQDCLRQQPHWGRVSLSGCFALFQYLTVSVDAAVVVLRQAADIPMTPETLDNVAQNASSPLQAITLMHTNLLRHHYKVSVYPLSPLRDILMSALKTYPCNVSLWKSYIRTEGKLHNASRVRRFIDSIRRMTPALEPCLFAIRAEEDRKKIVESVQRPDQGEVHSIFPETGLSNRIKALFEYALQSEYGSRCPLLWRLYLHFMVSLGNKERSKGLFYKAIQSCPWAKALYLDAVEYFPEQLQETIDLMTEKELRVRVPLEELDLLLED

Target ORF information:

RefSeq Version	NM_001079399.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079399.1

ORF Insert Sequence:

ATGGCTCTAT TTCCAGCCTT TGCAGACTCT TCTAGTTCTA GTAAGAAATC TCCTTCACGC 
CAAGAGTTGG ACTGGCTGAG CAACAAGAGC TTCTGCACAG AAGACGCTCT TTCACTACAC 
CAGCGTGCTT TGCAGTCTGC ACAATTTCCT CAACCTGCAT GCCACTCACC CAGGAGTCCA 
TTTCAGCAAG AGCTGTTTGA AGAAAGTGGA TCTGATTCTC AGGCAACAAA GAAGAAGAAG 
AAAAAGAAAA AAAAAAAGAA ACAGAAAAAA AAGCACAAGC AAAAGAGGGG TTATGATGAG 
GATGAAGAAG GGAATACAAG TGACTCTGAC TCAAGGCCTG AGACAAGGGA ATCTAAAGAG 
ACTGAAAGTG TGGCAAGGCA GGACACTTGT GTTGTGCTCC CTGGCCGTGG TATCTGGCTA 
GATGAAGTGC AGTCTGTGAC AGAGGAGACA TATCGAATTG ATAAGAAATC TGACCCTGCC 
AACTGGGAAT ATAAGTCACT GTACCAAGGA GACATAGCAC GGTACAAACG GAAAGGAAAT 
TCCTGTCTGG GCATCAATCC AAAGAAGCAG CAAATTAATT GGCAGGACGC ATTATCTGAG 
AAGAAAGAAG CACGCAGGAA AAATGAGCGT TATTTTACCA AGAGCACAAT GCAGCTTCTC 
AGTGCCAATG CAGATTTTGT GTCTTGTAAG GAAAGTGCAA GTCTGAAATC AGGGGAATTT 
ATTCCTGTGT TAGATCCTGA TAAGGATGGT GTTATTTCCT CCAGTGGCCC TATTAGCTGG 
GTGAATCCTT TGGGAGTGTA TGATTCTTCT ACAGCTCTGT GGTTGGAAGG AAAAGGGGGT 
CCAGAATATG ATACTATACA GCCAGTACCC CGGGACAACA ATATTTTGGC AAAGGTGGAG 
GATTATAACA AGCGGATCAG AGAATGCCCT AACGATATCC AACTATGGAT GGAGTTTGTC 
TCTTTTCAGG ATGAGTTGGT GAAGCAGCCC AGCATGTATT CTACCAGCAA AGGAGAGCTT 
GATGGCCATC GGACATCTGT GAAGCTGCTA CTGGAGAAAA AACTAGCTAT ACTAGAAAGA 
GCCATTGAGA GTAATCCGGG CAGCACTGAA TTAAAGCTTG CAAAGCTAAA GCTCTGTGAG 
GAATTCTGGG AGGCACCCAC TCTTCTCAAG GAATGGCAGA AACTGATCTT CTTGCACCCA 
AATGACTCGC AGCTGTGGCA GAAATATCTG CTCTTCTGCC AGGGTCAGTT TAGCACTTTC 
TCTGTGTCCA AGATGAACAG TATTTATGGC AAATGCCTAA GCACACTGGC AGCAGTTCAG 
GATGGTATTA TGCTTTCCCA CAATGCTCTG CCAGACACAG AGAGCAACAT GTATGATATT 
TTTCTGCAAC AGTGCCAGTT CTTACGCCAG GCTGGACACA CAGAGAAAGT AATATCCTTA 
TTCCAAGCTC TTACTGACTT CACATTTTAT AAACCGGACA GTGTTAAGGA TATGACAACC 
AAAGAGCAGG TTGACTTTTT TGAGCCTTTT TGGGACAGTG GGGAACCTCG TTTTGGTGAG 
AAAGGAGCAA AGGGATGGAG CTCCTGGATG CGCCAACAGG AGAGAGGGGG CTGGATCATC 
ATTAACAATT TGGTGGAGGA TGATGAAGAC GAAGCAGATG AAGAGTTAAG CATAAGAGGC 
AAAAACTGTC CCAGATACAA GATTTGGTTG GATGTTGAAT GCTCAAGGGA AGCAAGGCAC 
TGGTTGCCAT GGCGTCCAGA CACCACAAAA AAGCAGACAG AGGATGAATG TGAGGATCCA 
GAGAGGCAGG TTCTTTTTGA TGATATTGGC CCGTCAATGT TCAAGATCAC CAGTCCTCAA 
ATGCAGTTCC AGCTCTTTAA ATCATTCCTG CATTTTCTTG GGGTCCCCTG TGGGGCTAAC 
ATTTCCCCAC GCTGCTTTTA CCTTGCTCTT GATGAGACCT CCATCTTTGA TCATGTTTCT 
CCTTATGAAA TGCTGCTTAA CTCGTTTGAA ATCCCTCTGT CTGGAATCGG TACAGTTGGT 
CATTTAGACA CATTAAGCAG AAGCAGGCAA CAAATAGGAT ACTTTAAAGA AGGGGAAACC 
TTCATACAAA ATATCTTTCA GTCAGCTCTG TCACTTTTAC ATGGAGAAGA GAAGAGGAAC 
ATCTCAGTTT GGTGGCTTCA GTATGAAATT TCAAAGGTTG TTTGGTGCCT GCAAGCAAAT 
AACAAGAAAA AGCTGAGATC TCAGGGCAAG AGGAGCAAGC GATTGGCAAA GAGCCTACTG 
AAGGAAGCAT CAAACAGAAA CAGTCTGGCA CTCTGGAAGG AATATGCTCT TCTAGAATGG 
CTTCTGGGCA ATATTGATGA TGCCCGAAAG GTGTTTGATA CAGCCATAAG CTTAGCAGGA 
AGCAAAGGGT TAAAGGACCA GGAATTATGC AGTTTGTGTC TGCTGTATTC AGAACTGGAA 
GGGAAGCTAC TGGACAATCC AGAGGAAAAT GCGGGGTCCC GTGCTGTTCA TATACTTACC 
AGCCTTACAG AAAGCTCTCC TTACACTCCC TATAATAGCC CTGAGCATGC TATTAGTATT 
CTGAAAGCAC GCAAAGTATA TGAACACGCC ATGCAGGACT GCCTGAGGCA ACAGCCACAT 
TGGGGGCGTG TTAGCTTATC TGGATGCTTT GCACTTTTTC AGTATCTAAC TGTGAGCGTT 
GATGCTGCTG TTGTGGTATT AAGACAGGCT GCTGACATCC CAATGACTCC TGAAACATTG 
GACAATGTTG CTCAGAACGC CAGCAGCCCA TTGCAAGCAA TTACACTGAT GCACACAAAT 
CTGCTCAGAC ACCATTATAA GGTCAGTGTC TATCCTCTGA GTCCACTTAG AGACATACTG 
ATGTCTGCCT TAAAGACATA TCCCTGCAAT GTTTCTCTGT GGAAATCCTA CATCAGAACT 
GAAGGTAAAT TACACAATGC CAGCAGGGTG AGAAGGTTTA TAGACAGTAT CAGGAGAATG 
ACACCAGCTC TGGAACCATG TCTGTTCGCC ATCAGAGCAG AGGAGGACAG AAAAAAAATA 
GTAGAATCTG TACAAAGGCC AGATCAAGGG GAGGTTCACT CTATTTTCCC TGAAACTGGT 
TTGTCCAATC GAATCAAGGC CCTTTTTGAA TATGCTCTTC AAAGTGAATA TGGTTCCAGG 
TGCCCTCTGC TCTGGAGGCT GTACTTGCAC TTTATGGTGT CTTTGGGAAA CAAGGAGAGA 
AGCAAAGGCC TGTTCTACAA AGCAATACAG AGCTGCCCAT GGGCAAAGGC ACTATACTTG 
GATGCTGTAG AATATTTCCC GGAGCAGCTG CAAGAGACCA TTGATCTAAT GACTGAAAAG 
GAGCTGAGAG TACGAGTTCC CCTAGAGGAA CTGGATCTGC TGCTGGAAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrde2 ( XM_031890600.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746460.1 Xenopus tropicalis NRDE-2, necessary for RNA interference, domain containing (nrde2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63830
Clone ID Related Accession (Same CDS sequence)	XM_031890600.1
Accession Version	XM_031890600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear exosome regulator NRDE2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTAT TTCCAGCCTT TGCAGACTCT TCTAGTTCTA GTAAGAAATC TCCTTCACGC 
CAAGAGTTGG ACTGGCTGAG CAACAAGAGC TTCTGCACAG AAGACGCTCT TTCACTACAC 
CAGCGTGCTT TGCAGTCTGC ACAATTTCCT CAACCTGCAT GCCACTCACC CAGGAGTCCA 
TTTCAGCAAG AGCTGTTTGA AGAAAGTGGA TCTGATTCTC AGGCAACAAA GAAGAAGAAG 
AAAAAGAAAA AAAAAAAGAA ACAGAAAAAA AAGCACAAGC AAAAGAGGGG TTATGATGAG 
GATGAAGAAG GGAATACAAG TGACTCTGAC TCAAGGCCTG AGACAAGGGA ATCTAAAGAG 
ACTGAAAGTG TGGCAAGTAT CTCAATATTC AGTTTCTCTG CCAGGCAGGA CACTTGTGTT 
GTGCTCCCTG GCCGTGGTAT CTGGCTAGAT GAAGTGCAGT CTGTGACAGA GGAGACATAT 
CGAATTGATA AGAAATCTGA CCCTGCCAAC TGGGAATATA AGTCACTGTA CCAAGGAGAC 
ATAGCACGGT ACAAACGGAA AGGAAATTCC TGTCTGGGCA TCAATCCAAA GAAGCAGCAA 
ATTAATTGGC AGGACGCATT ATCTGAGAAG AAAGAAGCAC GCAGGAAAAA TGAGCGTTAT 
TTTACCAAGA GCACAATGCA GCTTCTCAGT GCCAATGCAG ATTTTGTGTC TTGTAAGGAA 
AGTGCAAGTC TGAAATCAGG GGAATTTATT CCTGTGTTAG ATCCTGATAA GGATGGTGTT 
ATTTCCTCCA GTGGCCCTAT TAGCTGGGTG AATCCTTTGG GAGTGTATGA TTCTTCTACA 
GCTCTGTGGT TGGAAGGAAA AGGGGGTCCA GAATATGATA CTATACAGCC AGTACCCCGG 
GACAACAATA TTTTGGCAAA GGTGGAGGAT TATAACAAGC GGATCAGAGA ATGCCCTAAC 
GATATCCAAC TATGGATGGA GTTTGTCTCT TTTCAGGATG AGTTGGTGAA GCAGCCCAGC 
ATGTATTCTA CCAGCAAAGG AGAGCTTGAT GGCCATCGGA CATCTGTGAA GCTGCTACTG 
GAGAAAAAAC TAGCTATACT AGAAAGAGCC ATTGAGAGTA ATCCGGGCAG CACTGAATTA 
AAGCTTGCAA AGCTAAAGCT CTGTGAGGAA TTCTGGGAGG CACCCACTCT TCTCAAGGAA 
TGGCAGAAAC TGATCTTCTT GCACCCAAAT GACTCGCAGC TGTGGCAGAA ATATCTGCTC 
TTCTGCCAGG GTCAGTTTAG CACTTTCTCT GTGTCCAAGA TGAACAGTAT TTATGGCAAA 
TGCCTAAGCA CACTGGCAGC AGTTCAGGAT GGTATTATGC TTTCCCACAA TGCTCTGCCA 
GACACAGAGA GCAACATGTA TGATATTTTT CTGCAACAGT GCCAGTTCTT ACGCCAGGCT 
GGACACACAG AGAAAGTAAT ATCCTTATTC CAAGCTCTTA CTGACTTCAC ATTTTATAAA 
CCGGACAGTG TTAAGGATAT GACAACCAAA GAGCAGGTTG ACTTTTTTGA GCCTTTTTGG 
GACAGTGGGG AACCTCGTTT TGGTGAGAAA GGAGCAAAGG GATGGAGCTC CTGGATGCGC 
CAACAGGAGA GAGGGGGCTG GATCATCATT AACAATTTGG TGGAGGATGA TGAAGACGAA 
GCAGATGAAG AGTTAAGCAT AAGAGGCAAA AACTGTCCCA GATACAAGAT TTGGTTGGAT 
GTTGAATGCT CAAGGGAAGC AAGGCACTGG TTGCCATGGC GTCCAGACAC CACAAAAAAG 
CAGACAGAGG ATGAATGTGA GGATCCAGAG AGGCAGGTTC TTTTTGATGA TATTGGCCCG 
TCAATGTTCA AGATCACCAG TCCTCAAATG CAGTTCCAGC TCTTTAAATC ATTCCTGCAT 
TTTCTTGGGG TCCCCTGTGG GGCTAACATT TCCCCACGCT GCTTTTACCT TGCTCTTGAT 
GAGACCTCCA TCTTTGATCA TGTTTCTCCT TATGAAATGC TGCTTAACTC GTTTGAAATC 
CCTCTGTCTG GAATCGGTAC AGTTGGTCAT TTAGACACAT TAAGCAGAAG CAGGCAACAA 
ATAGGATACT TTAAAGAAGG GGAAACCTTC ATACAAAATA TCTTTCAGTC AGCTCTGTCA 
CTTTTACATG GAGAAGAGAA GAGGAACATC TCAGTTTGGT GGCTTCAGTA TGAAATTTCA 
AAGGTTGTTT GGTGCCTGCA AGCAAATAAC AAGAAAAAGC TGAGATCTCA GGGCAAGAGG 
AGCAAGCGAT TGGCAAAGAG CCTACTGAAG GAAGCATCAA ACAGAAACAG TCTGGCACTC 
TGGAAGGAAT ATGCTCTTCT AGAATGGCTT CTGGGCAATA TTGATGATGC CCGAAAGGTG 
TTTGATACAG CCATAAGCTT AGCAGGAAGC AAAGGGTTAA AGGACCAGGA ATTATGCAGT 
TTGTGTCTGC TGTATTCAGA ACTGGAAGGG AAGCTACTGG ACAATCCAGA GGAAAATGCG 
GGGTCCCGTG CTGTTCATAT ACTTACCAGC CTTACAGAAA GCTCTCCTTA CACTCCCTAT 
AATAGCCCTG AGCATGCTAT TAGTATTCTG AAAGCACGCA AAGTATATGA ACACGCCATG 
CAGGACTGCC TGAGGCAACA GCCACATTGG GGGCGTGTTA GCTTATCTGG ATGCTTTGCA 
CTTTTTCAGT ATCTAACTGT GAGCGTTGAT GCTGCTGTTG TGGTATTAAG ACAGGCTGCT 
GACATCCCAA TGACTCCTGA AACATTGGAC AATGTTGCTC AGAACGCCAG CAGCCCATTG 
CAAGCAATTA CACTGATGCA CACAAATCTG CTCAGACACC ATTATAAGGT CAGTGTCTAT 
CCTCTGAGTC CACTTAGAGA CATACTGATG TCTGCCTTAA AGACATATCC CTGCAATGTT 
TCTCTGTGGA AATCCTACAT CAGAACTGAA GGTAAATTAC ACAATGCCAG CAGGGTGAGA 
AGGTTTATAG ACAGTATCAG GAGAATGACA CCAGCTCTGG AACCATGTCT GTTCGCCATC 
AGAGCAGAGG AGGACAGAAA AAAAATAGTA GAATCTGTAC AAAGGCCAGA TCAAGGGGAG 
GTTCACTCTA TTTTCCCTGA AACTGGTTTG TCCAATCGAA TCAAGGCCCT TTTTGAATAT 
GCTCTTCAAA GTGAATATGG TTCCAGGTGC CCTCTGCTCT GGAGGCTGTA CTTGCACTTT 
ATGGTGTCTT TGGGAAACAA GGAGAGAAGC AAAGGCCTGT TCTACAAAGC AATACAGAGC 
TGCCCATGGG CAAAGGCACT ATACTTGGAT GCTGTAGAAT ATTTCCCGGA GCAGCTGCAA 
GAGACCATTG ATCTAATGAC TGAAAAGGAG CTGAGAGTAC GAGTTCCCCT AGAGGAACTG 
GATCTGCTGC TGGAAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746460.1
CDS	105..3545
Translation	MALFPAFADSSSSSKKSPSRQELDWLSNKSFCTEDALSLHQRALQSAQFPQPACHSPRSPFQQELFEESGSDSQATKKKKKKKKKKKQKKKHKQKRGYDEDEEGNTSDSDSRPETRESKETESVASISIFSFSARQDTCVVLPGRGIWLDEVQSVTEETYRIDKKSDPANWEYKSLYQGDIARYKRKGNSCLGINPKKQQINWQDALSEKKEARRKNERYFTKSTMQLLSANADFVSCKESASLKSGEFIPVLDPDKDGVISSSGPISWVNPLGVYDSSTALWLEGKGGPEYDTIQPVPRDNNILAKVEDYNKRIRECPNDIQLWMEFVSFQDELVKQPSMYSTSKGELDGHRTSVKLLLEKKLAILERAIESNPGSTELKLAKLKLCEEFWEAPTLLKEWQKLIFLHPNDSQLWQKYLLFCQGQFSTFSVSKMNSIYGKCLSTLAAVQDGIMLSHNALPDTESNMYDIFLQQCQFLRQAGHTEKVISLFQALTDFTFYKPDSVKDMTTKEQVDFFEPFWDSGEPRFGEKGAKGWSSWMRQQERGGWIIINNLVEDDEDEADEELSIRGKNCPRYKIWLDVECSREARHWLPWRPDTTKKQTEDECEDPERQVLFDDIGPSMFKITSPQMQFQLFKSFLHFLGVPCGANISPRCFYLALDETSIFDHVSPYEMLLNSFEIPLSGIGTVGHLDTLSRSRQQIGYFKEGETFIQNIFQSALSLLHGEEKRNISVWWLQYEISKVVWCLQANNKKKLRSQGKRSKRLAKSLLKEASNRNSLALWKEYALLEWLLGNIDDARKVFDTAISLAGSKGLKDQELCSLCLLYSELEGKLLDNPEENAGSRAVHILTSLTESSPYTPYNSPEHAISILKARKVYEHAMQDCLRQQPHWGRVSLSGCFALFQYLTVSVDAAVVVLRQAADIPMTPETLDNVAQNASSPLQAITLMHTNLLRHHYKVSVYPLSPLRDILMSALKTYPCNVSLWKSYIRTEGKLHNASRVRRFIDSIRRMTPALEPCLFAIRAEEDRKKIVESVQRPDQGEVHSIFPETGLSNRIKALFEYALQSEYGSRCPLLWRLYLHFMVSLGNKERSKGLFYKAIQSCPWAKALYLDAVEYFPEQLQETIDLMTEKELRVRVPLEELDLLLED

Target ORF information:

RefSeq Version	XM_031890600.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NRDE-2, necessary for RNA interference, domain containing (nrde2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890600.1

ORF Insert Sequence:

ATGGCTCTAT TTCCAGCCTT TGCAGACTCT TCTAGTTCTA GTAAGAAATC TCCTTCACGC 
CAAGAGTTGG ACTGGCTGAG CAACAAGAGC TTCTGCACAG AAGACGCTCT TTCACTACAC 
CAGCGTGCTT TGCAGTCTGC ACAATTTCCT CAACCTGCAT GCCACTCACC CAGGAGTCCA 
TTTCAGCAAG AGCTGTTTGA AGAAAGTGGA TCTGATTCTC AGGCAACAAA GAAGAAGAAG 
AAAAAGAAAA AAAAAAAGAA ACAGAAAAAA AAGCACAAGC AAAAGAGGGG TTATGATGAG 
GATGAAGAAG GGAATACAAG TGACTCTGAC TCAAGGCCTG AGACAAGGGA ATCTAAAGAG 
ACTGAAAGTG TGGCAAGTAT CTCAATATTC AGTTTCTCTG CCAGGCAGGA CACTTGTGTT 
GTGCTCCCTG GCCGTGGTAT CTGGCTAGAT GAAGTGCAGT CTGTGACAGA GGAGACATAT 
CGAATTGATA AGAAATCTGA CCCTGCCAAC TGGGAATATA AGTCACTGTA CCAAGGAGAC 
ATAGCACGGT ACAAACGGAA AGGAAATTCC TGTCTGGGCA TCAATCCAAA GAAGCAGCAA 
ATTAATTGGC AGGACGCATT ATCTGAGAAG AAAGAAGCAC GCAGGAAAAA TGAGCGTTAT 
TTTACCAAGA GCACAATGCA GCTTCTCAGT GCCAATGCAG ATTTTGTGTC TTGTAAGGAA 
AGTGCAAGTC TGAAATCAGG GGAATTTATT CCTGTGTTAG ATCCTGATAA GGATGGTGTT 
ATTTCCTCCA GTGGCCCTAT TAGCTGGGTG AATCCTTTGG GAGTGTATGA TTCTTCTACA 
GCTCTGTGGT TGGAAGGAAA AGGGGGTCCA GAATATGATA CTATACAGCC AGTACCCCGG 
GACAACAATA TTTTGGCAAA GGTGGAGGAT TATAACAAGC GGATCAGAGA ATGCCCTAAC 
GATATCCAAC TATGGATGGA GTTTGTCTCT TTTCAGGATG AGTTGGTGAA GCAGCCCAGC 
ATGTATTCTA CCAGCAAAGG AGAGCTTGAT GGCCATCGGA CATCTGTGAA GCTGCTACTG 
GAGAAAAAAC TAGCTATACT AGAAAGAGCC ATTGAGAGTA ATCCGGGCAG CACTGAATTA 
AAGCTTGCAA AGCTAAAGCT CTGTGAGGAA TTCTGGGAGG CACCCACTCT TCTCAAGGAA 
TGGCAGAAAC TGATCTTCTT GCACCCAAAT GACTCGCAGC TGTGGCAGAA ATATCTGCTC 
TTCTGCCAGG GTCAGTTTAG CACTTTCTCT GTGTCCAAGA TGAACAGTAT TTATGGCAAA 
TGCCTAAGCA CACTGGCAGC AGTTCAGGAT GGTATTATGC TTTCCCACAA TGCTCTGCCA 
GACACAGAGA GCAACATGTA TGATATTTTT CTGCAACAGT GCCAGTTCTT ACGCCAGGCT 
GGACACACAG AGAAAGTAAT ATCCTTATTC CAAGCTCTTA CTGACTTCAC ATTTTATAAA 
CCGGACAGTG TTAAGGATAT GACAACCAAA GAGCAGGTTG ACTTTTTTGA GCCTTTTTGG 
GACAGTGGGG AACCTCGTTT TGGTGAGAAA GGAGCAAAGG GATGGAGCTC CTGGATGCGC 
CAACAGGAGA GAGGGGGCTG GATCATCATT AACAATTTGG TGGAGGATGA TGAAGACGAA 
GCAGATGAAG AGTTAAGCAT AAGAGGCAAA AACTGTCCCA GATACAAGAT TTGGTTGGAT 
GTTGAATGCT CAAGGGAAGC AAGGCACTGG TTGCCATGGC GTCCAGACAC CACAAAAAAG 
CAGACAGAGG ATGAATGTGA GGATCCAGAG AGGCAGGTTC TTTTTGATGA TATTGGCCCG 
TCAATGTTCA AGATCACCAG TCCTCAAATG CAGTTCCAGC TCTTTAAATC ATTCCTGCAT 
TTTCTTGGGG TCCCCTGTGG GGCTAACATT TCCCCACGCT GCTTTTACCT TGCTCTTGAT 
GAGACCTCCA TCTTTGATCA TGTTTCTCCT TATGAAATGC TGCTTAACTC GTTTGAAATC 
CCTCTGTCTG GAATCGGTAC AGTTGGTCAT TTAGACACAT TAAGCAGAAG CAGGCAACAA 
ATAGGATACT TTAAAGAAGG GGAAACCTTC ATACAAAATA TCTTTCAGTC AGCTCTGTCA 
CTTTTACATG GAGAAGAGAA GAGGAACATC TCAGTTTGGT GGCTTCAGTA TGAAATTTCA 
AAGGTTGTTT GGTGCCTGCA AGCAAATAAC AAGAAAAAGC TGAGATCTCA GGGCAAGAGG 
AGCAAGCGAT TGGCAAAGAG CCTACTGAAG GAAGCATCAA ACAGAAACAG TCTGGCACTC 
TGGAAGGAAT ATGCTCTTCT AGAATGGCTT CTGGGCAATA TTGATGATGC CCGAAAGGTG 
TTTGATACAG CCATAAGCTT AGCAGGAAGC AAAGGGTTAA AGGACCAGGA ATTATGCAGT 
TTGTGTCTGC TGTATTCAGA ACTGGAAGGG AAGCTACTGG ACAATCCAGA GGAAAATGCG 
GGGTCCCGTG CTGTTCATAT ACTTACCAGC CTTACAGAAA GCTCTCCTTA CACTCCCTAT 
AATAGCCCTG AGCATGCTAT TAGTATTCTG AAAGCACGCA AAGTATATGA ACACGCCATG 
CAGGACTGCC TGAGGCAACA GCCACATTGG GGGCGTGTTA GCTTATCTGG ATGCTTTGCA 
CTTTTTCAGT ATCTAACTGT GAGCGTTGAT GCTGCTGTTG TGGTATTAAG ACAGGCTGCT 
GACATCCCAA TGACTCCTGA AACATTGGAC AATGTTGCTC AGAACGCCAG CAGCCCATTG 
CAAGCAATTA CACTGATGCA CACAAATCTG CTCAGACACC ATTATAAGGT CAGTGTCTAT 
CCTCTGAGTC CACTTAGAGA CATACTGATG TCTGCCTTAA AGACATATCC CTGCAATGTT 
TCTCTGTGGA AATCCTACAT CAGAACTGAA GGTAAATTAC ACAATGCCAG CAGGGTGAGA 
AGGTTTATAG ACAGTATCAG GAGAATGACA CCAGCTCTGG AACCATGTCT GTTCGCCATC 
AGAGCAGAGG AGGACAGAAA AAAAATAGTA GAATCTGTAC AAAGGCCAGA TCAAGGGGAG 
GTTCACTCTA TTTTCCCTGA AACTGGTTTG TCCAATCGAA TCAAGGCCCT TTTTGAATAT 
GCTCTTCAAA GTGAATATGG TTCCAGGTGC CCTCTGCTCT GGAGGCTGTA CTTGCACTTT 
ATGGTGTCTT TGGGAAACAA GGAGAGAAGC AAAGGCCTGT TCTACAAAGC AATACAGAGC 
TGCCCATGGG CAAAGGCACT ATACTTGGAT GCTGTAGAAT ATTTCCCGGA GCAGCTGCAA 
GAGACCATTG ATCTAATGAC TGAAAAGGAG CTGAGAGTAC GAGTTCCCCT AGAGGAACTG 
GATCTGCTGC TGGAAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrde2 ( XM_031890601.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746461.1 Xenopus tropicalis NRDE-2, necessary for RNA interference, domain containing (nrde2), transcript variant X2, mRNA.

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CloneID	OXa63831
Clone ID Related Accession (Same CDS sequence)	XM_031890601.1
Accession Version	XM_031890601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nuclear exosome regulator NRDE2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTAT TTCCAGCCTT TGCAGACTCT TCTAGTTCTA GTAAGAAATC TCCTTCACGC 
CAAGAGTTGG ACTGGCTGAG CAACAAGAGC TTCTGCACAG AAGACGCTCT TTCACTACAC 
CAGCGTGCTT TGCAGTCTGC ACAATTTCCT CAACCTGCAT GCCACTCACC CAGGAGTCCA 
TTTCAGCAAG AGCTGTTTGA AGAAAGTGGA TCTGATTCTC AGGCAACAAA GAAGAAGAAG 
AAAAAGAAAA AAAAAAAGAA ACAGAAAAAA AAGCACAAGC AAAAGAGGGG TTATGATGAG 
GATGAAGAAG GGAATACAAG TGACTCTGAC TCAAGGCCTG AGACAAGGGA ATCTAAAGAG 
ACTGAAAGTG TGGCAAGTTT CTCTGCCAGG CAGGACACTT GTGTTGTGCT CCCTGGCCGT 
GGTATCTGGC TAGATGAAGT GCAGTCTGTG ACAGAGGAGA CATATCGAAT TGATAAGAAA 
TCTGACCCTG CCAACTGGGA ATATAAGTCA CTGTACCAAG GAGACATAGC ACGGTACAAA 
CGGAAAGGAA ATTCCTGTCT GGGCATCAAT CCAAAGAAGC AGCAAATTAA TTGGCAGGAC 
GCATTATCTG AGAAGAAAGA AGCACGCAGG AAAAATGAGC GTTATTTTAC CAAGAGCACA 
ATGCAGCTTC TCAGTGCCAA TGCAGATTTT GTGTCTTGTA AGGAAAGTGC AAGTCTGAAA 
TCAGGGGAAT TTATTCCTGT GTTAGATCCT GATAAGGATG GTGTTATTTC CTCCAGTGGC 
CCTATTAGCT GGGTGAATCC TTTGGGAGTG TATGATTCTT CTACAGCTCT GTGGTTGGAA 
GGAAAAGGGG GTCCAGAATA TGATACTATA CAGCCAGTAC CCCGGGACAA CAATATTTTG 
GCAAAGGTGG AGGATTATAA CAAGCGGATC AGAGAATGCC CTAACGATAT CCAACTATGG 
ATGGAGTTTG TCTCTTTTCA GGATGAGTTG GTGAAGCAGC CCAGCATGTA TTCTACCAGC 
AAAGGAGAGC TTGATGGCCA TCGGACATCT GTGAAGCTGC TACTGGAGAA AAAACTAGCT 
ATACTAGAAA GAGCCATTGA GAGTAATCCG GGCAGCACTG AATTAAAGCT TGCAAAGCTA 
AAGCTCTGTG AGGAATTCTG GGAGGCACCC ACTCTTCTCA AGGAATGGCA GAAACTGATC 
TTCTTGCACC CAAATGACTC GCAGCTGTGG CAGAAATATC TGCTCTTCTG CCAGGGTCAG 
TTTAGCACTT TCTCTGTGTC CAAGATGAAC AGTATTTATG GCAAATGCCT AAGCACACTG 
GCAGCAGTTC AGGATGGTAT TATGCTTTCC CACAATGCTC TGCCAGACAC AGAGAGCAAC 
ATGTATGATA TTTTTCTGCA ACAGTGCCAG TTCTTACGCC AGGCTGGACA CACAGAGAAA 
GTAATATCCT TATTCCAAGC TCTTACTGAC TTCACATTTT ATAAACCGGA CAGTGTTAAG 
GATATGACAA CCAAAGAGCA GGTTGACTTT TTTGAGCCTT TTTGGGACAG TGGGGAACCT 
CGTTTTGGTG AGAAAGGAGC AAAGGGATGG AGCTCCTGGA TGCGCCAACA GGAGAGAGGG 
GGCTGGATCA TCATTAACAA TTTGGTGGAG GATGATGAAG ACGAAGCAGA TGAAGAGTTA 
AGCATAAGAG GCAAAAACTG TCCCAGATAC AAGATTTGGT TGGATGTTGA ATGCTCAAGG 
GAAGCAAGGC ACTGGTTGCC ATGGCGTCCA GACACCACAA AAAAGCAGAC AGAGGATGAA 
TGTGAGGATC CAGAGAGGCA GGTTCTTTTT GATGATATTG GCCCGTCAAT GTTCAAGATC 
ACCAGTCCTC AAATGCAGTT CCAGCTCTTT AAATCATTCC TGCATTTTCT TGGGGTCCCC 
TGTGGGGCTA ACATTTCCCC ACGCTGCTTT TACCTTGCTC TTGATGAGAC CTCCATCTTT 
GATCATGTTT CTCCTTATGA AATGCTGCTT AACTCGTTTG AAATCCCTCT GTCTGGAATC 
GGTACAGTTG GTCATTTAGA CACATTAAGC AGAAGCAGGC AACAAATAGG ATACTTTAAA 
GAAGGGGAAA CCTTCATACA AAATATCTTT CAGTCAGCTC TGTCACTTTT ACATGGAGAA 
GAGAAGAGGA ACATCTCAGT TTGGTGGCTT CAGTATGAAA TTTCAAAGGT TGTTTGGTGC 
CTGCAAGCAA ATAACAAGAA AAAGCTGAGA TCTCAGGGCA AGAGGAGCAA GCGATTGGCA 
AAGAGCCTAC TGAAGGAAGC ATCAAACAGA AACAGTCTGG CACTCTGGAA GGAATATGCT 
CTTCTAGAAT GGCTTCTGGG CAATATTGAT GATGCCCGAA AGGTGTTTGA TACAGCCATA 
AGCTTAGCAG GAAGCAAAGG GTTAAAGGAC CAGGAATTAT GCAGTTTGTG TCTGCTGTAT 
TCAGAACTGG AAGGGAAGCT ACTGGACAAT CCAGAGGAAA ATGCGGGGTC CCGTGCTGTT 
CATATACTTA CCAGCCTTAC AGAAAGCTCT CCTTACACTC CCTATAATAG CCCTGAGCAT 
GCTATTAGTA TTCTGAAAGC ACGCAAAGTA TATGAACACG CCATGCAGGA CTGCCTGAGG 
CAACAGCCAC ATTGGGGGCG TGTTAGCTTA TCTGGATGCT TTGCACTTTT TCAGTATCTA 
ACTGTGAGCG TTGATGCTGC TGTTGTGGTA TTAAGACAGG CTGCTGACAT CCCAATGACT 
CCTGAAACAT TGGACAATGT TGCTCAGAAC GCCAGCAGCC CATTGCAAGC AATTACACTG 
ATGCACACAA ATCTGCTCAG ACACCATTAT AAGGTCAGTG TCTATCCTCT GAGTCCACTT 
AGAGACATAC TGATGTCTGC CTTAAAGACA TATCCCTGCA ATGTTTCTCT GTGGAAATCC 
TACATCAGAA CTGAAGGTAA ATTACACAAT GCCAGCAGGG TGAGAAGGTT TATAGACAGT 
ATCAGGAGAA TGACACCAGC TCTGGAACCA TGTCTGTTCG CCATCAGAGC AGAGGAGGAC 
AGAAAAAAAA TAGTAGAATC TGTACAAAGG CCAGATCAAG GGGAGGTTCA CTCTATTTTC 
CCTGAAACTG GTTTGTCCAA TCGAATCAAG GCCCTTTTTG AATATGCTCT TCAAAGTGAA 
TATGGTTCCA GGTGCCCTCT GCTCTGGAGG CTGTACTTGC ACTTTATGGT GTCTTTGGGA 
AACAAGGAGA GAAGCAAAGG CCTGTTCTAC AAAGCAATAC AGAGCTGCCC ATGGGCAAAG 
GCACTATACT TGGATGCTGT AGAATATTTC CCGGAGCAGC TGCAAGAGAC CATTGATCTA 
ATGACTGAAA AGGAGCTGAG AGTACGAGTT CCCCTAGAGG AACTGGATCT GCTGCTGGAA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746461.1
CDS	105..3530
Translation	MALFPAFADSSSSSKKSPSRQELDWLSNKSFCTEDALSLHQRALQSAQFPQPACHSPRSPFQQELFEESGSDSQATKKKKKKKKKKKQKKKHKQKRGYDEDEEGNTSDSDSRPETRESKETESVASFSARQDTCVVLPGRGIWLDEVQSVTEETYRIDKKSDPANWEYKSLYQGDIARYKRKGNSCLGINPKKQQINWQDALSEKKEARRKNERYFTKSTMQLLSANADFVSCKESASLKSGEFIPVLDPDKDGVISSSGPISWVNPLGVYDSSTALWLEGKGGPEYDTIQPVPRDNNILAKVEDYNKRIRECPNDIQLWMEFVSFQDELVKQPSMYSTSKGELDGHRTSVKLLLEKKLAILERAIESNPGSTELKLAKLKLCEEFWEAPTLLKEWQKLIFLHPNDSQLWQKYLLFCQGQFSTFSVSKMNSIYGKCLSTLAAVQDGIMLSHNALPDTESNMYDIFLQQCQFLRQAGHTEKVISLFQALTDFTFYKPDSVKDMTTKEQVDFFEPFWDSGEPRFGEKGAKGWSSWMRQQERGGWIIINNLVEDDEDEADEELSIRGKNCPRYKIWLDVECSREARHWLPWRPDTTKKQTEDECEDPERQVLFDDIGPSMFKITSPQMQFQLFKSFLHFLGVPCGANISPRCFYLALDETSIFDHVSPYEMLLNSFEIPLSGIGTVGHLDTLSRSRQQIGYFKEGETFIQNIFQSALSLLHGEEKRNISVWWLQYEISKVVWCLQANNKKKLRSQGKRSKRLAKSLLKEASNRNSLALWKEYALLEWLLGNIDDARKVFDTAISLAGSKGLKDQELCSLCLLYSELEGKLLDNPEENAGSRAVHILTSLTESSPYTPYNSPEHAISILKARKVYEHAMQDCLRQQPHWGRVSLSGCFALFQYLTVSVDAAVVVLRQAADIPMTPETLDNVAQNASSPLQAITLMHTNLLRHHYKVSVYPLSPLRDILMSALKTYPCNVSLWKSYIRTEGKLHNASRVRRFIDSIRRMTPALEPCLFAIRAEEDRKKIVESVQRPDQGEVHSIFPETGLSNRIKALFEYALQSEYGSRCPLLWRLYLHFMVSLGNKERSKGLFYKAIQSCPWAKALYLDAVEYFPEQLQETIDLMTEKELRVRVPLEELDLLLED

Target ORF information:

RefSeq Version	XM_031890601.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NRDE-2, necessary for RNA interference, domain containing (nrde2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890601.1

ORF Insert Sequence:

ATGGCTCTAT TTCCAGCCTT TGCAGACTCT TCTAGTTCTA GTAAGAAATC TCCTTCACGC 
CAAGAGTTGG ACTGGCTGAG CAACAAGAGC TTCTGCACAG AAGACGCTCT TTCACTACAC 
CAGCGTGCTT TGCAGTCTGC ACAATTTCCT CAACCTGCAT GCCACTCACC CAGGAGTCCA 
TTTCAGCAAG AGCTGTTTGA AGAAAGTGGA TCTGATTCTC AGGCAACAAA GAAGAAGAAG 
AAAAAGAAAA AAAAAAAGAA ACAGAAAAAA AAGCACAAGC AAAAGAGGGG TTATGATGAG 
GATGAAGAAG GGAATACAAG TGACTCTGAC TCAAGGCCTG AGACAAGGGA ATCTAAAGAG 
ACTGAAAGTG TGGCAAGTTT CTCTGCCAGG CAGGACACTT GTGTTGTGCT CCCTGGCCGT 
GGTATCTGGC TAGATGAAGT GCAGTCTGTG ACAGAGGAGA CATATCGAAT TGATAAGAAA 
TCTGACCCTG CCAACTGGGA ATATAAGTCA CTGTACCAAG GAGACATAGC ACGGTACAAA 
CGGAAAGGAA ATTCCTGTCT GGGCATCAAT CCAAAGAAGC AGCAAATTAA TTGGCAGGAC 
GCATTATCTG AGAAGAAAGA AGCACGCAGG AAAAATGAGC GTTATTTTAC CAAGAGCACA 
ATGCAGCTTC TCAGTGCCAA TGCAGATTTT GTGTCTTGTA AGGAAAGTGC AAGTCTGAAA 
TCAGGGGAAT TTATTCCTGT GTTAGATCCT GATAAGGATG GTGTTATTTC CTCCAGTGGC 
CCTATTAGCT GGGTGAATCC TTTGGGAGTG TATGATTCTT CTACAGCTCT GTGGTTGGAA 
GGAAAAGGGG GTCCAGAATA TGATACTATA CAGCCAGTAC CCCGGGACAA CAATATTTTG 
GCAAAGGTGG AGGATTATAA CAAGCGGATC AGAGAATGCC CTAACGATAT CCAACTATGG 
ATGGAGTTTG TCTCTTTTCA GGATGAGTTG GTGAAGCAGC CCAGCATGTA TTCTACCAGC 
AAAGGAGAGC TTGATGGCCA TCGGACATCT GTGAAGCTGC TACTGGAGAA AAAACTAGCT 
ATACTAGAAA GAGCCATTGA GAGTAATCCG GGCAGCACTG AATTAAAGCT TGCAAAGCTA 
AAGCTCTGTG AGGAATTCTG GGAGGCACCC ACTCTTCTCA AGGAATGGCA GAAACTGATC 
TTCTTGCACC CAAATGACTC GCAGCTGTGG CAGAAATATC TGCTCTTCTG CCAGGGTCAG 
TTTAGCACTT TCTCTGTGTC CAAGATGAAC AGTATTTATG GCAAATGCCT AAGCACACTG 
GCAGCAGTTC AGGATGGTAT TATGCTTTCC CACAATGCTC TGCCAGACAC AGAGAGCAAC 
ATGTATGATA TTTTTCTGCA ACAGTGCCAG TTCTTACGCC AGGCTGGACA CACAGAGAAA 
GTAATATCCT TATTCCAAGC TCTTACTGAC TTCACATTTT ATAAACCGGA CAGTGTTAAG 
GATATGACAA CCAAAGAGCA GGTTGACTTT TTTGAGCCTT TTTGGGACAG TGGGGAACCT 
CGTTTTGGTG AGAAAGGAGC AAAGGGATGG AGCTCCTGGA TGCGCCAACA GGAGAGAGGG 
GGCTGGATCA TCATTAACAA TTTGGTGGAG GATGATGAAG ACGAAGCAGA TGAAGAGTTA 
AGCATAAGAG GCAAAAACTG TCCCAGATAC AAGATTTGGT TGGATGTTGA ATGCTCAAGG 
GAAGCAAGGC ACTGGTTGCC ATGGCGTCCA GACACCACAA AAAAGCAGAC AGAGGATGAA 
TGTGAGGATC CAGAGAGGCA GGTTCTTTTT GATGATATTG GCCCGTCAAT GTTCAAGATC 
ACCAGTCCTC AAATGCAGTT CCAGCTCTTT AAATCATTCC TGCATTTTCT TGGGGTCCCC 
TGTGGGGCTA ACATTTCCCC ACGCTGCTTT TACCTTGCTC TTGATGAGAC CTCCATCTTT 
GATCATGTTT CTCCTTATGA AATGCTGCTT AACTCGTTTG AAATCCCTCT GTCTGGAATC 
GGTACAGTTG GTCATTTAGA CACATTAAGC AGAAGCAGGC AACAAATAGG ATACTTTAAA 
GAAGGGGAAA CCTTCATACA AAATATCTTT CAGTCAGCTC TGTCACTTTT ACATGGAGAA 
GAGAAGAGGA ACATCTCAGT TTGGTGGCTT CAGTATGAAA TTTCAAAGGT TGTTTGGTGC 
CTGCAAGCAA ATAACAAGAA AAAGCTGAGA TCTCAGGGCA AGAGGAGCAA GCGATTGGCA 
AAGAGCCTAC TGAAGGAAGC ATCAAACAGA AACAGTCTGG CACTCTGGAA GGAATATGCT 
CTTCTAGAAT GGCTTCTGGG CAATATTGAT GATGCCCGAA AGGTGTTTGA TACAGCCATA 
AGCTTAGCAG GAAGCAAAGG GTTAAAGGAC CAGGAATTAT GCAGTTTGTG TCTGCTGTAT 
TCAGAACTGG AAGGGAAGCT ACTGGACAAT CCAGAGGAAA ATGCGGGGTC CCGTGCTGTT 
CATATACTTA CCAGCCTTAC AGAAAGCTCT CCTTACACTC CCTATAATAG CCCTGAGCAT 
GCTATTAGTA TTCTGAAAGC ACGCAAAGTA TATGAACACG CCATGCAGGA CTGCCTGAGG 
CAACAGCCAC ATTGGGGGCG TGTTAGCTTA TCTGGATGCT TTGCACTTTT TCAGTATCTA 
ACTGTGAGCG TTGATGCTGC TGTTGTGGTA TTAAGACAGG CTGCTGACAT CCCAATGACT 
CCTGAAACAT TGGACAATGT TGCTCAGAAC GCCAGCAGCC CATTGCAAGC AATTACACTG 
ATGCACACAA ATCTGCTCAG ACACCATTAT AAGGTCAGTG TCTATCCTCT GAGTCCACTT 
AGAGACATAC TGATGTCTGC CTTAAAGACA TATCCCTGCA ATGTTTCTCT GTGGAAATCC 
TACATCAGAA CTGAAGGTAA ATTACACAAT GCCAGCAGGG TGAGAAGGTT TATAGACAGT 
ATCAGGAGAA TGACACCAGC TCTGGAACCA TGTCTGTTCG CCATCAGAGC AGAGGAGGAC 
AGAAAAAAAA TAGTAGAATC TGTACAAAGG CCAGATCAAG GGGAGGTTCA CTCTATTTTC 
CCTGAAACTG GTTTGTCCAA TCGAATCAAG GCCCTTTTTG AATATGCTCT TCAAAGTGAA 
TATGGTTCCA GGTGCCCTCT GCTCTGGAGG CTGTACTTGC ACTTTATGGT GTCTTTGGGA 
AACAAGGAGA GAAGCAAAGG CCTGTTCTAC AAAGCAATAC AGAGCTGCCC ATGGGCAAAG 
GCACTATACT TGGATGCTGT AGAATATTTC CCGGAGCAGC TGCAAGAGAC CATTGATCTA 
ATGACTGAAA AGGAGCTGAG AGTACGAGTT CCCCTAGAGG AACTGGATCT GCTGCTGGAA 
GATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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nrde2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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