NRDE2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NRDE2
Entrez Gene ID	55051
Full Name	NRDE-2, necessary for RNA interference, domain containing
Synonyms	C14orf102
General protein information	Preferred Names NRDE-2, necessary for RNA interference, domain containing Names protein NRDE2 homolog UPF0614 protein C14orf102
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.11

mRNA and Protein(s)

mRNA	Protein	Name
NM_017970.4	NP_060440.2	nuclear exosome regulator NRDE2
XM_017021394.2	XP_016876883.1	nuclear exosome regulator NRDE2 isoform X2
XM_017021395.1	XP_016876884.1	nuclear exosome regulator NRDE2 isoform X3
XM_017021396.1	XP_016876885.1	nuclear exosome regulator NRDE2 isoform X4
XM_011536886.2	XP_011535188.1	nuclear exosome regulator NRDE2 isoform X1
XM_017021394.1	XP_016876883.1	protein NRDE2 homolog isoform X2
XM_011536886.1	XP_011535188.1	protein NRDE2 homolog isoform X1
NM_017970.3	NP_060440.2	protein NRDE2 homolog

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	NRDE2	XP_001090586.1
Rattus norvegicus (Norway rat)	Nrde2	XP_234465.5
Arabidopsis thaliana (thale cress)	AT3G17740	NP_188401.1
Homo sapiens (human)	NRDE2	NP_060440.2
Gallus gallus (chicken)	C5H14ORF102	XP_421314.3
Arabidopsis thaliana (thale cress)	AT3G17712	NP_001154620.1
Oryza sativa (rice)	Os02g0772500	NP_001048265.1
Mus musculus (house mouse)	Nrde2	NP_898978.2
Xenopus tropicalis (tropical clawed frog)	nrde2	NP_001072867.1
Canis lupus familiaris (dog)	NRDE2	XP_547952.3
Bos taurus (cattle)	NRDE2	NP_001193436.1
Pan troglodytes (chimpanzee)	NRDE2	XP_001141630.1
Danio rerio (zebrafish)	LOC794348	XP_005158613.1

Gene Ontology
Bibliography

Related articles in PubMed

NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.
Wang J, Chen J, Wu G, Zhang H, Du X, Chen S, Zhang L, Wang K, Fan J, Gao S, Wu X, Zhang S, Kuai B, Zhao P, Chi B, Wang L, Li G, Wong CCL, Zhou Y, Li J, Yun C, Cheng H
Genes & development33(9-10)536-549(2019 05)

Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor.
Jiao AL, Perales R, Umbreit NT, Haswell JR, Piper ME, Adams BD, Pellman D, Kennedy S, Slack FJ
RNA (New York, N.Y.)25(3)352-363(2019 03)

NRDE-2, the human homolog of fission yeast Nrl1, prevents DNA damage accumulation in human cells.
Richard P, Ogami K, Chen Y, Feng S, Moresco JJ, Yates JR 3rd, Manley JL
RNA biology15(7)868-876(2018)

Joint influence of small-effect genetic variants on human longevity.
Yashin AI, Wu D, Arbeev KG, Ukraintseva SV
Aging2(9)612-20(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NRDE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRDE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu68933	XM_017021396.1 Latest version!	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu84961	XM_017021395.1 Latest version!	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu68932	XM_011536886.2 Latest version!	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu84960	XM_017021394.2 Latest version!	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu02637	NM_017970.4 Latest version!	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
View Old Accession Versions >>
OHu84960	XM_017021394.1	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu68932	XM_011536886.1	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu02637	NM_017970.3	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NRDE2 ( XM_017021396.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876885.1 Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu68933
Clone ID Related Accession (Same CDS sequence)	XM_017021396.1
Accession Version	XM_017021396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	nuclear exosome regulator NRDE2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT TCACCTTCTT CAAACCCGAC AGCGTGAAAG ATCTGCCTAC CAAAGGACAG 
GTGGAATTCT TTGAACCCTT TTGGGACAGT GGAGAGCCCC GGGCTGGGGA GAAGGGAGCC 
CGAGGCTGGA AGGCGTGGAT GCACCAGCAG GAACGAGGTG GCTGGGTGGT CATCAACCCA 
GATGAGGATG ACGATGAACC AGAAGAGGAT GACCAGGAAA TAAAAGATAA GACTCTGCCC 
AGGTGGCAGA TCTGGCTTGC TGCTGAGCGT TCCCGTGACC AGAGGCACTG GCGGCCCTGG 
CGCCCTGATA AGACCAAGAA GCAAACCGAG GAAGACTGTG AGGATCCCGA GAGACAGGTG 
TTGTTTGATG ATATTGGGCA ATCTTTGATC AGACTTTCCA GCCATGATCT TCAGTTCCAG 
CTGGTGGAGG CCTTCCTGCA GTTCTTGGGT GTGCCTTCTG GCTTTACTCC TCCAGCCTCC 
TGTCTTTATC TGGCCATGGA TGAGAACAGC ATCTTTGATA ATGGACTTTA TGATGAAAAG 
CCCTTGACTT TTTTCAACCC TTTGTTTTCT GGGGCTAGCT GTGTTGGCCG CATGGATAGG 
TTGGGCTATC CTCGCTGGAC CAGGGGTCAG AACCGAGAGG GCGAGGAGTT CATCCGCAAT 
GTCTTCCACC TTGTCATGCC TTTATTTTCA GGCAAAGAGA AGTCCCAGCT CTGCTTCTCC 
TGGTTACAGT ATGAGATTGC AAAGGTCATT TGGTGCCTGC ACACTAAAAA CAAGAAGAGA 
TTAAAGTCTC AAGGGAAGAA CTGCAAAAAA CTAGCCAAGA ATCTCCTTAA GGAGCCAGAA 
AACTGCAACA ACTTTTGCCT GTGGAAGCAG TATGCACATC TGGAGTGGTT GCTTGGCAAC 
ACGGAGGATG CCAGAAAAGT TTTTGACACA GCACTTGGCA TGGCAGGAAG CAGAGAACTG 
AAAGACTCTG ACCTCTGTGA GCTCAGTCTG CTCTATGCTG AGCTGGAGGT GGAGCTGTCG 
CCAGAAGTGA GAAGGGCTGC CACAGCTCGA GCTGTTCACA TATTAACCAA GCTGACTGAG 
AGCAGCCCCT ATGGGCCCTA CACTGGACAG GTGTTGGCTG TTCACATTTT GAAAGCGCGA 
AAGGCTTATG AGCACGCACT GCAGGACTGT TTGGGTGACA GCTGTGTCTC CAATCCAGCT 
CCCACCGATT CCTGTAGCCG CCTAATTAGC CTGGCTAAAT GCTTCATGCT CTTCCAGTAT 
TTGACCATAG GGATTGATGC TGCTGTGCAG ATATACGAAC AGGTGTTTGC AAAACTGAAC 
AGTTCTGTTT TCCCAGAAGG CTCTGGCGAG GGGGACAGTG CCAGCTCCCA GAGTTGGACC 
AGTGTTCTCG AAGCCATCAC ACTGATGCAC ACGAGCCTGC TGAGATTCCA CATGAAAGTG 
AGTGTTTACC CGCTGGCCCC TCTGCGAGAG GCACTCTCAC AGGCTTTAAA GTTGTATCCA 
GGCAACCAGG TTCTTTGGAG GTCCTATGTA CAGATTCAGA ATAAGTCCCA CAGTGCCAGC 
AAAACCAGGA GATTTTTTGA CACAATCACC AGGTCTGCCA AACCCTTGGA GCCTTGGTTG 
TTTGCAATTG AAGCTGAGAA ACTGAGGAAG AGACTGGTGG AAACTGTCCA GAGGTTAGAC 
GGTAGAGAGA TCCACGCCAC AATTCCTGAG ACCGGCTTAA TGCATCGGAT CCAAGCCCTG 
TTTGAAAATG CCATGCGCAG CGACAGTGGC AGCCAGTGCC CCTTGCTGTG GAGGATGTAT 
TTGAACTTTC TGGTTTCCTT AGGAAATAAA GAAAGAAGCA AAGGTGTATT CTACAAAGCA 
CTTCAGAATT GCCCTTGGGC AAAGGTGTTG TACCTGGACG CCGTGGAGTA TTTCCCCGAT 
GAGATGCAGG AGATCCTGGA CCTGATGACT GAGAAGGAGC TCCGGGTGCG CCTGCCGCTG 
GAGGAGCTGG AGCTGCTGCT GGAGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876885.1
CDS	1262..3271
Translation	MVDFTFFKPDSVKDLPTKGQVEFFEPFWDSGEPRAGEKGARGWKAWMHQQERGGWVVINPDEDDDEPEEDDQEIKDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSHDLQFQLVEAFLQFLGVPSGFTPPASCLYLAMDENSIFDNGLYDEKPLTFFNPLFSGASCVGRMDRLGYPRWTRGQNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENCNNFCLWKQYAHLEWLLGNTEDARKVFDTALGMAGSRELKDSDLCELSLLYAELEVELSPEVRRAATARAVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKVLYLDAVEYFPDEMQEILDLMTEKELRVRLPLEELELLLED

Target ORF information:

RefSeq Version	XM_017021396.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021396.1

ORF Insert Sequence:

ATGGTGGACT TCACCTTCTT CAAACCCGAC AGCGTGAAAG ATCTGCCTAC CAAAGGACAG 
GTGGAATTCT TTGAACCCTT TTGGGACAGT GGAGAGCCCC GGGCTGGGGA GAAGGGAGCC 
CGAGGCTGGA AGGCGTGGAT GCACCAGCAG GAACGAGGTG GCTGGGTGGT CATCAACCCA 
GATGAGGATG ACGATGAACC AGAAGAGGAT GACCAGGAAA TAAAAGATAA GACTCTGCCC 
AGGTGGCAGA TCTGGCTTGC TGCTGAGCGT TCCCGTGACC AGAGGCACTG GCGGCCCTGG 
CGCCCTGATA AGACCAAGAA GCAAACCGAG GAAGACTGTG AGGATCCCGA GAGACAGGTG 
TTGTTTGATG ATATTGGGCA ATCTTTGATC AGACTTTCCA GCCATGATCT TCAGTTCCAG 
CTGGTGGAGG CCTTCCTGCA GTTCTTGGGT GTGCCTTCTG GCTTTACTCC TCCAGCCTCC 
TGTCTTTATC TGGCCATGGA TGAGAACAGC ATCTTTGATA ATGGACTTTA TGATGAAAAG 
CCCTTGACTT TTTTCAACCC TTTGTTTTCT GGGGCTAGCT GTGTTGGCCG CATGGATAGG 
TTGGGCTATC CTCGCTGGAC CAGGGGTCAG AACCGAGAGG GCGAGGAGTT CATCCGCAAT 
GTCTTCCACC TTGTCATGCC TTTATTTTCA GGCAAAGAGA AGTCCCAGCT CTGCTTCTCC 
TGGTTACAGT ATGAGATTGC AAAGGTCATT TGGTGCCTGC ACACTAAAAA CAAGAAGAGA 
TTAAAGTCTC AAGGGAAGAA CTGCAAAAAA CTAGCCAAGA ATCTCCTTAA GGAGCCAGAA 
AACTGCAACA ACTTTTGCCT GTGGAAGCAG TATGCACATC TGGAGTGGTT GCTTGGCAAC 
ACGGAGGATG CCAGAAAAGT TTTTGACACA GCACTTGGCA TGGCAGGAAG CAGAGAACTG 
AAAGACTCTG ACCTCTGTGA GCTCAGTCTG CTCTATGCTG AGCTGGAGGT GGAGCTGTCG 
CCAGAAGTGA GAAGGGCTGC CACAGCTCGA GCTGTTCACA TATTAACCAA GCTGACTGAG 
AGCAGCCCCT ATGGGCCCTA CACTGGACAG GTGTTGGCTG TTCACATTTT GAAAGCGCGA 
AAGGCTTATG AGCACGCACT GCAGGACTGT TTGGGTGACA GCTGTGTCTC CAATCCAGCT 
CCCACCGATT CCTGTAGCCG CCTAATTAGC CTGGCTAAAT GCTTCATGCT CTTCCAGTAT 
TTGACCATAG GGATTGATGC TGCTGTGCAG ATATACGAAC AGGTGTTTGC AAAACTGAAC 
AGTTCTGTTT TCCCAGAAGG CTCTGGCGAG GGGGACAGTG CCAGCTCCCA GAGTTGGACC 
AGTGTTCTCG AAGCCATCAC ACTGATGCAC ACGAGCCTGC TGAGATTCCA CATGAAAGTG 
AGTGTTTACC CGCTGGCCCC TCTGCGAGAG GCACTCTCAC AGGCTTTAAA GTTGTATCCA 
GGCAACCAGG TTCTTTGGAG GTCCTATGTA CAGATTCAGA ATAAGTCCCA CAGTGCCAGC 
AAAACCAGGA GATTTTTTGA CACAATCACC AGGTCTGCCA AACCCTTGGA GCCTTGGTTG 
TTTGCAATTG AAGCTGAGAA ACTGAGGAAG AGACTGGTGG AAACTGTCCA GAGGTTAGAC 
GGTAGAGAGA TCCACGCCAC AATTCCTGAG ACCGGCTTAA TGCATCGGAT CCAAGCCCTG 
TTTGAAAATG CCATGCGCAG CGACAGTGGC AGCCAGTGCC CCTTGCTGTG GAGGATGTAT 
TTGAACTTTC TGGTTTCCTT AGGAAATAAA GAAAGAAGCA AAGGTGTATT CTACAAAGCA 
CTTCAGAATT GCCCTTGGGC AAAGGTGTTG TACCTGGACG CCGTGGAGTA TTTCCCCGAT 
GAGATGCAGG AGATCCTGGA CCTGATGACT GAGAAGGAGC TCCGGGTGCG CCTGCCGCTG 
GAGGAGCTGG AGCTGCTGCT GGAGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRDE2 ( XM_017021395.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876884.1 Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu84961
Clone ID Related Accession (Same CDS sequence)	XM_017021395.1
Accession Version	XM_017021395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	nuclear exosome regulator NRDE2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATCG ATGGAGTTGC CATTAGCAGT AAAACTGAAC CTCCCTCATC TGAGCCCATC 
TCCTTTATAC CAGTGAAGGA CTTGGAAGAT GCGGCTCCTG TTACAACCTG GTTGAATCCT 
CTGGGGATTT ATGATCAGTC AACCACACAT TGGCTACAAG GACAGGGTCC TCCAGAGCAG 
GAATCAAAGC AGCCAGACGC ACAGCCAGAC AGCGAGAGTG CGGCTCTCAA GGCCAAGGTG 
GAGGAGTTTA ACAGGAGGGT GCGGGAGAAT CCTCGGGATA CGCAGCTGTG GATGGCATTT 
GTTGCTTTTC AGGACGAGGT CATGAAAAGT CCTGGCCTGT ATGCCATCGA GGAAGGAGAG 
CAGGAAAAGC GAAAGAGGTC CCTGAAGCTC ATTCTGGAGA AGAAGCTGGC CATTCTGGAG 
CGGGCCATTG AGAGCAACCA GAGCAGTGTG GATCTGAAAC TGGCCAAGCT GAAGCTCTGC 
ACAGAGTTCT GGGAGCCCTC CACTCTGGTC AAAGAGTGGC AGAAACTGAT ATTTTTGCAT 
CCCAATAATA CAGCCCTTTG GCAGAAATAC CTTTTATTTT GCCAGAGCCA GTTTAGTACC 
TTTTCGATAT CAAAAATTCA CAGTCTTTAT GGAAAATGCT TGAGCACTTT GTCTGCTGTT 
AAGGACGGCA GCATCTTATC TCACCCTGCG TTGCCTGGCA CGGAAGAGGC CATGTTTGCA 
CTCTTTCTTC AGCAGTGCCA CTTTCTGCGG CAGGCTGGCC ACTCTGAGAA GGCCATCTCA 
TTGTTCCAGG CCATGGTGGA CTTCACCTTC TTCAAACCCG ACAGCGTGAA AGATCTGCCT 
ACCAAAGGAC AGGTGGAATT CTTTGAACCC TTTTGGGACA GTGGAGAGCC CCGGGCTGGG 
GAGAAGGGAG CCCGAGGCTG GAAGGCGTGG ATGCACCAGC AGGAACGAGG TGGCTGGGTG 
GTCATCAACC CAGATGAGGA TGACGATGAA CCAGAAGAGG ATGACCAGGA AATAAAAGAT 
AAGACTCTGC CCAGGTGGCA GATCTGGCTT GCTGCTGAGC GTTCCCGTGA CCAGAGGCAC 
TGGCGGCCCT GGCGCCCTGA TAAGACCAAG AAGCAAACCG AGGAAGACTG TGAGGATCCC 
GAGAGACAGG TGTTGTTTGA TGATATTGGG CAATCTTTGA TCAGACTTTC CAGCCATGAT 
CTTCAGTTCC AGCTGGTGGA GGCCTTCCTG CAGTTCTTGG GTGTGCCTTC TGGCTTTACT 
CCTCCAGCCT CCTGTCTTTA TCTGGCCATG GATGAGAACA GCATCTTTGA TAATGGACTT 
TATGATGAAA AGCCCTTGAC TTTTTTCAAC CCTTTGTTTT CTGGGGCTAG CTGTGTTGGC 
CGCATGGATA GGTTGGGCTA TCCTCGCTGG ACCAGGGGTC AGAACCGAGA GGGCGAGGAG 
TTCATCCGCA ATGTCTTCCA CCTTGTCATG CCTTTATTTT CAGGCAAAGA GAAGTCCCAG 
CTCTGCTTCT CCTGGTTACA GTATGAGATT GCAAAGGTCA TTTGGTGCCT GCACACTAAA 
AACAAGAAGA GATTAAAGTC TCAAGGGAAG AACTGCAAAA AACTAGCCAA GAATCTCCTT 
AAGGAGCCAG AAAACTGCAA CAACTTTTGC CTGTGGAAGC AGTATGCACA TCTGGAGTGG 
TTGCTTGGCA ACACGGAGGA TGCCAGAAAA GTTTTTGACA CAGCACTTGG CATGGCAGGA 
AGCAGAGAAC TGAAAGACTC TGACCTCTGT GAGCTCAGTC TGCTCTATGC TGAGCTGGAG 
GTGGAGCTGT CGCCAGAAGT GAGAAGGGCT GCCACAGCTC GAGCTGTTCA CATATTAACC 
AAGCTGACTG AGAGCAGCCC CTATGGGCCC TACACTGGAC AGGTGTTGGC TGTTCACATT 
TTGAAAGCGC GAAAGGCTTA TGAGCACGCA CTGCAGGACT GTTTGGGTGA CAGCTGTGTC 
TCCAATCCAG CTCCCACCGA TTCCTGTAGC CGCCTAATTA GCCTGGCTAA ATGCTTCATG 
CTCTTCCAGT ATTTGACCAT AGGGATTGAT GCTGCTGTGC AGATATACGA ACAGGTGTTT 
GCAAAACTGA ACAGTTCTGT TTTCCCAGAA GGCTCTGGCG AGGGGGACAG TGCCAGCTCC 
CAGAGTTGGA CCAGTGTTCT CGAAGCCATC ACACTGATGC ACACGAGCCT GCTGAGATTC 
CACATGAAAG TGAGTGTTTA CCCGCTGGCC CCTCTGCGAG AGGCACTCTC ACAGGCTTTA 
AAGTTGTATC CAGGCAACCA GGTTCTTTGG AGGTCCTATG TACAGATTCA GAATAAGTCC 
CACAGTGCCA GCAAAACCAG GAGATTTTTT GACACAATCA CCAGGTCTGC CAAACCCTTG 
GAGCCTTGGT TGTTTGCAAT TGAAGCTGAG AAACTGAGGA AGAGACTGGT GGAAACTGTC 
CAGAGGTTAG ACGGTAGAGA GATCCACGCC ACAATTCCTG AGACCGGCTT AATGCATCGG 
ATCCAAGCCC TGTTTGAAAA TGCCATGCGC AGCGACAGTG GCAGCCAGTG CCCCTTGCTG 
TGGAGGATGT ATTTGAACTT TCTGGTTTCC TTAGGAAATA AAGAAAGAAG CAAAGGTGTA 
TTCTACAAAG CACTTCAGAA TTGCCCTTGG GCAAAGGTGT TGTACCTGGA CGCCGTGGAG 
TATTTCCCCG ATGAGATGCA GGAGATCCTG GACCTGATGA CTGAGAAGGA GCTCCGGGTG 
CGCCTGCCGC TGGAGGAGCT GGAGCTGCTG CTGGAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876884.1
CDS	876..3677
Translation	MNIDGVAISSKTEPPSSEPISFIPVKDLEDAAPVTTWLNPLGIYDQSTTHWLQGQGPPEQESKQPDAQPDSESAALKAKVEEFNRRVRENPRDTQLWMAFVAFQDEVMKSPGLYAIEEGEQEKRKRSLKLILEKKLAILERAIESNQSSVDLKLAKLKLCTEFWEPSTLVKEWQKLIFLHPNNTALWQKYLLFCQSQFSTFSISKIHSLYGKCLSTLSAVKDGSILSHPALPGTEEAMFALFLQQCHFLRQAGHSEKAISLFQAMVDFTFFKPDSVKDLPTKGQVEFFEPFWDSGEPRAGEKGARGWKAWMHQQERGGWVVINPDEDDDEPEEDDQEIKDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSHDLQFQLVEAFLQFLGVPSGFTPPASCLYLAMDENSIFDNGLYDEKPLTFFNPLFSGASCVGRMDRLGYPRWTRGQNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENCNNFCLWKQYAHLEWLLGNTEDARKVFDTALGMAGSRELKDSDLCELSLLYAELEVELSPEVRRAATARAVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKVLYLDAVEYFPDEMQEILDLMTEKELRVRLPLEELELLLED

Target ORF information:

RefSeq Version	XM_017021395.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021395.1

ORF Insert Sequence:

ATGAACATCG ATGGAGTTGC CATTAGCAGT AAAACTGAAC CTCCCTCATC TGAGCCCATC 
TCCTTTATAC CAGTGAAGGA CTTGGAAGAT GCGGCTCCTG TTACAACCTG GTTGAATCCT 
CTGGGGATTT ATGATCAGTC AACCACACAT TGGCTACAAG GACAGGGTCC TCCAGAGCAG 
GAATCAAAGC AGCCAGACGC ACAGCCAGAC AGCGAGAGTG CGGCTCTCAA GGCCAAGGTG 
GAGGAGTTTA ACAGGAGGGT GCGGGAGAAT CCTCGGGATA CGCAGCTGTG GATGGCATTT 
GTTGCTTTTC AGGACGAGGT CATGAAAAGT CCTGGCCTGT ATGCCATCGA GGAAGGAGAG 
CAGGAAAAGC GAAAGAGGTC CCTGAAGCTC ATTCTGGAGA AGAAGCTGGC CATTCTGGAG 
CGGGCCATTG AGAGCAACCA GAGCAGTGTG GATCTGAAAC TGGCCAAGCT GAAGCTCTGC 
ACAGAGTTCT GGGAGCCCTC CACTCTGGTC AAAGAGTGGC AGAAACTGAT ATTTTTGCAT 
CCCAATAATA CAGCCCTTTG GCAGAAATAC CTTTTATTTT GCCAGAGCCA GTTTAGTACC 
TTTTCGATAT CAAAAATTCA CAGTCTTTAT GGAAAATGCT TGAGCACTTT GTCTGCTGTT 
AAGGACGGCA GCATCTTATC TCACCCTGCG TTGCCTGGCA CGGAAGAGGC CATGTTTGCA 
CTCTTTCTTC AGCAGTGCCA CTTTCTGCGG CAGGCTGGCC ACTCTGAGAA GGCCATCTCA 
TTGTTCCAGG CCATGGTGGA CTTCACCTTC TTCAAACCCG ACAGCGTGAA AGATCTGCCT 
ACCAAAGGAC AGGTGGAATT CTTTGAACCC TTTTGGGACA GTGGAGAGCC CCGGGCTGGG 
GAGAAGGGAG CCCGAGGCTG GAAGGCGTGG ATGCACCAGC AGGAACGAGG TGGCTGGGTG 
GTCATCAACC CAGATGAGGA TGACGATGAA CCAGAAGAGG ATGACCAGGA AATAAAAGAT 
AAGACTCTGC CCAGGTGGCA GATCTGGCTT GCTGCTGAGC GTTCCCGTGA CCAGAGGCAC 
TGGCGGCCCT GGCGCCCTGA TAAGACCAAG AAGCAAACCG AGGAAGACTG TGAGGATCCC 
GAGAGACAGG TGTTGTTTGA TGATATTGGG CAATCTTTGA TCAGACTTTC CAGCCATGAT 
CTTCAGTTCC AGCTGGTGGA GGCCTTCCTG CAGTTCTTGG GTGTGCCTTC TGGCTTTACT 
CCTCCAGCCT CCTGTCTTTA TCTGGCCATG GATGAGAACA GCATCTTTGA TAATGGACTT 
TATGATGAAA AGCCCTTGAC TTTTTTCAAC CCTTTGTTTT CTGGGGCTAG CTGTGTTGGC 
CGCATGGATA GGTTGGGCTA TCCTCGCTGG ACCAGGGGTC AGAACCGAGA GGGCGAGGAG 
TTCATCCGCA ATGTCTTCCA CCTTGTCATG CCTTTATTTT CAGGCAAAGA GAAGTCCCAG 
CTCTGCTTCT CCTGGTTACA GTATGAGATT GCAAAGGTCA TTTGGTGCCT GCACACTAAA 
AACAAGAAGA GATTAAAGTC TCAAGGGAAG AACTGCAAAA AACTAGCCAA GAATCTCCTT 
AAGGAGCCAG AAAACTGCAA CAACTTTTGC CTGTGGAAGC AGTATGCACA TCTGGAGTGG 
TTGCTTGGCA ACACGGAGGA TGCCAGAAAA GTTTTTGACA CAGCACTTGG CATGGCAGGA 
AGCAGAGAAC TGAAAGACTC TGACCTCTGT GAGCTCAGTC TGCTCTATGC TGAGCTGGAG 
GTGGAGCTGT CGCCAGAAGT GAGAAGGGCT GCCACAGCTC GAGCTGTTCA CATATTAACC 
AAGCTGACTG AGAGCAGCCC CTATGGGCCC TACACTGGAC AGGTGTTGGC TGTTCACATT 
TTGAAAGCGC GAAAGGCTTA TGAGCACGCA CTGCAGGACT GTTTGGGTGA CAGCTGTGTC 
TCCAATCCAG CTCCCACCGA TTCCTGTAGC CGCCTAATTA GCCTGGCTAA ATGCTTCATG 
CTCTTCCAGT ATTTGACCAT AGGGATTGAT GCTGCTGTGC AGATATACGA ACAGGTGTTT 
GCAAAACTGA ACAGTTCTGT TTTCCCAGAA GGCTCTGGCG AGGGGGACAG TGCCAGCTCC 
CAGAGTTGGA CCAGTGTTCT CGAAGCCATC ACACTGATGC ACACGAGCCT GCTGAGATTC 
CACATGAAAG TGAGTGTTTA CCCGCTGGCC CCTCTGCGAG AGGCACTCTC ACAGGCTTTA 
AAGTTGTATC CAGGCAACCA GGTTCTTTGG AGGTCCTATG TACAGATTCA GAATAAGTCC 
CACAGTGCCA GCAAAACCAG GAGATTTTTT GACACAATCA CCAGGTCTGC CAAACCCTTG 
GAGCCTTGGT TGTTTGCAAT TGAAGCTGAG AAACTGAGGA AGAGACTGGT GGAAACTGTC 
CAGAGGTTAG ACGGTAGAGA GATCCACGCC ACAATTCCTG AGACCGGCTT AATGCATCGG 
ATCCAAGCCC TGTTTGAAAA TGCCATGCGC AGCGACAGTG GCAGCCAGTG CCCCTTGCTG 
TGGAGGATGT ATTTGAACTT TCTGGTTTCC TTAGGAAATA AAGAAAGAAG CAAAGGTGTA 
TTCTACAAAG CACTTCAGAA TTGCCCTTGG GCAAAGGTGT TGTACCTGGA CGCCGTGGAG 
TATTTCCCCG ATGAGATGCA GGAGATCCTG GACCTGATGA CTGAGAAGGA GCTCCGGGTG 
CGCCTGCCGC TGGAGGAGCT GGAGCTGCTG CTGGAGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRDE2 ( XM_011536886.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535188.1 Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X1, mRNA.

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CloneID	OHu68932
Clone ID Related Accession (Same CDS sequence)	XM_011536886.2 , XM_011536886.1
Accession Version	XM_011536886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	nuclear exosome regulator NRDE2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011536886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCCGACTCTG AACATAGGAT GAAAGTTGCT 
GCACTCTTTC TTCAGCAGTG CCACTTTCTG CGGCAGGCTG GCCACTCTGA GAAGGCCATC 
TCATTGTTCC AGGCCATGGT GGACTTCACC TTCTTCAAAC CCGACAGCGT GAAAGATCTG 
CCTACCAAAG GACAGGTGGA ATTCTTTGAA CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT 
GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG TGGATGCACC AGCAGGAACG AGGTGGCTGG 
GTGGTCATCA ACCCAGATGA GGATGACGAT GAACCAGAAG AGGATGACCA GGAAATAAAA 
GATAAGACTC TGCCCAGGTG GCAGATCTGG CTTGCTGCTG AGCGTTCCCG TGACCAGAGG 
CACTGGCGGC CCTGGCGCCC TGATAAGACC AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT 
CCCGAGAGAC AGGTGTTGTT TGATGATATT GGGCAATCTT TGATCAGACT TTCCAGCCAT 
GATCTTCAGT TCCAGCTGGT GGAGGCCTTC CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT 
ACTCCTCCAG CCTCCTGTCT TTATCTGGCC ATGGATGAGA ACAGCATCTT TGATAATGGA 
CTTTATGATG AAAAGCCCTT GACTTTTTTC AACCCTTTGT TTTCTGGGGC TAGCTGTGTT 
GGCCGCATGG ATAGGTTGGG CTATCCTCGC TGGACCAGGG GTCAGAACCG AGAGGGCGAG 
GAGTTCATCC GCAATGTCTT CCACCTTGTC ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC 
CAGCTCTGCT TCTCCTGGTT ACAGTATGAG ATTGCAAAGG TCATTTGGTG CCTGCACACT 
AAAAACAAGA AGAGATTAAA GTCTCAAGGG AAGAACTGCA AAAAACTAGC CAAGAATCTC 
CTTAAGGAGC CAGAAAACTG CAACAACTTT TGCCTGTGGA AGCAGTATGC ACATCTGGAG 
TGGTTGCTTG GCAACACGGA GGATGCCAGA AAAGTTTTTG ACACAGCACT TGGCATGGCA 
GGAAGCAGAG AACTGAAAGA CTCTGACCTC TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG 
GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG GCTGCCACAG CTCGAGCTGT TCACATATTA 
ACCAAGCTGA CTGAGAGCAG CCCCTATGGG CCCTACACTG GACAGGTGTT GGCTGTTCAC 
ATTTTGAAAG CGCGAAAGGC TTATGAGCAC GCACTGCAGG ACTGTTTGGG TGACAGCTGT 
GTCTCCAATC CAGCTCCCAC CGATTCCTGT AGCCGCCTAA TTAGCCTGGC TAAATGCTTC 
ATGCTCTTCC AGTATTTGAC CATAGGGATT GATGCTGCTG TGCAGATATA CGAACAGGTG 
TTTGCAAAAC TGAACAGTTC TGTTTTCCCA GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC 
TCCCAGAGTT GGACCAGTGT TCTCGAAGCC ATCACACTGA TGCACACGAG CCTGCTGAGA 
TTCCACATGA AAGTGAGTGT TTACCCGCTG GCCCCTCTGC GAGAGGCACT CTCACAGGCT 
TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT TGGAGGTCCT ATGTACAGAT TCAGAATAAG 
TCCCACAGTG CCAGCAAAAC CAGGAGATTT TTTGACACAA TCACCAGGTC TGCCAAACCC 
TTGGAGCCTT GGTTGTTTGC AATTGAAGCT GAGAAACTGA GGAAGAGACT GGTGGAAACT 
GTCCAGAGGT TAGACGGTAG AGAGATCCAC GCCACAATTC CTGAGACCGG CTTAATGCAT 
CGGATCCAAG CCCTGTTTGA AAATGCCATG CGCAGCGACA GTGGCAGCCA GTGCCCCTTG 
CTGTGGAGGA TGTATTTGAA CTTTCTGGTT TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT 
GTATTCTACA AAGCACTTCA GAATTGCCCT TGGGCAAAGG TGTTGTACCT GGACGCCGTG 
GAGTATTTCC CCGATGAGAT GCAGGAGATC CTGGACCTGA TGACTGAGAA GGAGCTCCGG 
GTGCGCCTGC CGCTGGAGGA GCTGGAGCTG CTGCTGGAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535188.1
CDS	33..3557
Translation	MALFPAFAGLSEAPDGGSSRKELDWLSNPSFCVGSITSLSQQTEAAPAHVSEGLPLTRSHLKSESSDESDTNKKLKQTSRKKKKEKKKKRKHQHHKKTKRKHGPSSSSRSETDTDSEKDKPSRGVGGSKKESEEPNQGNNAAADTGHRFVWLEDIQAVTGETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGTSTEKKHSRKQVERYFTKKSVGLMNIDGVAISSKTEPPSSEPISFIPVKDLEDAAPVTTWLNPLGIYDQSTTHWLQGQGPPEQESKQPDAQPDSESAALKAKVEEFNRRVRENPRDTQLWMAFVAFQDEVMKSPGLYAIEEGEQEKRKRSLKLILEKKLAILERAIESNQSSVDLKLAKLKLCTEFWEPSTLVKEWQKLIFLHPNNTALWQKYLLFCQSQFSTFSISKIHSLYGKCLSTLSAVKDGSILSHPALPGTEEAMFADSEHRMKVAALFLQQCHFLRQAGHSEKAISLFQAMVDFTFFKPDSVKDLPTKGQVEFFEPFWDSGEPRAGEKGARGWKAWMHQQERGGWVVINPDEDDDEPEEDDQEIKDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSHDLQFQLVEAFLQFLGVPSGFTPPASCLYLAMDENSIFDNGLYDEKPLTFFNPLFSGASCVGRMDRLGYPRWTRGQNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENCNNFCLWKQYAHLEWLLGNTEDARKVFDTALGMAGSRELKDSDLCELSLLYAELEVELSPEVRRAATARAVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKVLYLDAVEYFPDEMQEILDLMTEKELRVRLPLEELELLLED

Target ORF information:

RefSeq Version	XM_011536886.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536886.2

ORF Insert Sequence:

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCCGACTCTG AACATAGGAT GAAAGTTGCT 
GCACTCTTTC TTCAGCAGTG CCACTTTCTG CGGCAGGCTG GCCACTCTGA GAAGGCCATC 
TCATTGTTCC AGGCCATGGT GGACTTCACC TTCTTCAAAC CCGACAGCGT GAAAGATCTG 
CCTACCAAAG GACAGGTGGA ATTCTTTGAA CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT 
GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG TGGATGCACC AGCAGGAACG AGGTGGCTGG 
GTGGTCATCA ACCCAGATGA GGATGACGAT GAACCAGAAG AGGATGACCA GGAAATAAAA 
GATAAGACTC TGCCCAGGTG GCAGATCTGG CTTGCTGCTG AGCGTTCCCG TGACCAGAGG 
CACTGGCGGC CCTGGCGCCC TGATAAGACC AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT 
CCCGAGAGAC AGGTGTTGTT TGATGATATT GGGCAATCTT TGATCAGACT TTCCAGCCAT 
GATCTTCAGT TCCAGCTGGT GGAGGCCTTC CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT 
ACTCCTCCAG CCTCCTGTCT TTATCTGGCC ATGGATGAGA ACAGCATCTT TGATAATGGA 
CTTTATGATG AAAAGCCCTT GACTTTTTTC AACCCTTTGT TTTCTGGGGC TAGCTGTGTT 
GGCCGCATGG ATAGGTTGGG CTATCCTCGC TGGACCAGGG GTCAGAACCG AGAGGGCGAG 
GAGTTCATCC GCAATGTCTT CCACCTTGTC ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC 
CAGCTCTGCT TCTCCTGGTT ACAGTATGAG ATTGCAAAGG TCATTTGGTG CCTGCACACT 
AAAAACAAGA AGAGATTAAA GTCTCAAGGG AAGAACTGCA AAAAACTAGC CAAGAATCTC 
CTTAAGGAGC CAGAAAACTG CAACAACTTT TGCCTGTGGA AGCAGTATGC ACATCTGGAG 
TGGTTGCTTG GCAACACGGA GGATGCCAGA AAAGTTTTTG ACACAGCACT TGGCATGGCA 
GGAAGCAGAG AACTGAAAGA CTCTGACCTC TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG 
GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG GCTGCCACAG CTCGAGCTGT TCACATATTA 
ACCAAGCTGA CTGAGAGCAG CCCCTATGGG CCCTACACTG GACAGGTGTT GGCTGTTCAC 
ATTTTGAAAG CGCGAAAGGC TTATGAGCAC GCACTGCAGG ACTGTTTGGG TGACAGCTGT 
GTCTCCAATC CAGCTCCCAC CGATTCCTGT AGCCGCCTAA TTAGCCTGGC TAAATGCTTC 
ATGCTCTTCC AGTATTTGAC CATAGGGATT GATGCTGCTG TGCAGATATA CGAACAGGTG 
TTTGCAAAAC TGAACAGTTC TGTTTTCCCA GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC 
TCCCAGAGTT GGACCAGTGT TCTCGAAGCC ATCACACTGA TGCACACGAG CCTGCTGAGA 
TTCCACATGA AAGTGAGTGT TTACCCGCTG GCCCCTCTGC GAGAGGCACT CTCACAGGCT 
TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT TGGAGGTCCT ATGTACAGAT TCAGAATAAG 
TCCCACAGTG CCAGCAAAAC CAGGAGATTT TTTGACACAA TCACCAGGTC TGCCAAACCC 
TTGGAGCCTT GGTTGTTTGC AATTGAAGCT GAGAAACTGA GGAAGAGACT GGTGGAAACT 
GTCCAGAGGT TAGACGGTAG AGAGATCCAC GCCACAATTC CTGAGACCGG CTTAATGCAT 
CGGATCCAAG CCCTGTTTGA AAATGCCATG CGCAGCGACA GTGGCAGCCA GTGCCCCTTG 
CTGTGGAGGA TGTATTTGAA CTTTCTGGTT TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT 
GTATTCTACA AAGCACTTCA GAATTGCCCT TGGGCAAAGG TGTTGTACCT GGACGCCGTG 
GAGTATTTCC CCGATGAGAT GCAGGAGATC CTGGACCTGA TGACTGAGAA GGAGCTCCGG 
GTGCGCCTGC CGCTGGAGGA GCTGGAGCTG CTGCTGGAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRDE2 ( XM_011536886.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535188.1 Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu68932
Clone ID Related Accession (Same CDS sequence)	XM_011536886.2 , XM_011536886.1
Accession Version	XM_011536886.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	protein NRDE2 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCCGACTCTG AACATAGGAT GAAAGTTGCT 
GCACTCTTTC TTCAGCAGTG CCACTTTCTG CGGCAGGCTG GCCACTCTGA GAAGGCCATC 
TCATTGTTCC AGGCCATGGT GGACTTCACC TTCTTCAAAC CCGACAGCGT GAAAGATCTG 
CCTACCAAAG GACAGGTGGA ATTCTTTGAA CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT 
GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG TGGATGCACC AGCAGGAACG AGGTGGCTGG 
GTGGTCATCA ACCCAGATGA GGATGACGAT GAACCAGAAG AGGATGACCA GGAAATAAAA 
GATAAGACTC TGCCCAGGTG GCAGATCTGG CTTGCTGCTG AGCGTTCCCG TGACCAGAGG 
CACTGGCGGC CCTGGCGCCC TGATAAGACC AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT 
CCCGAGAGAC AGGTGTTGTT TGATGATATT GGGCAATCTT TGATCAGACT TTCCAGCCAT 
GATCTTCAGT TCCAGCTGGT GGAGGCCTTC CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT 
ACTCCTCCAG CCTCCTGTCT TTATCTGGCC ATGGATGAGA ACAGCATCTT TGATAATGGA 
CTTTATGATG AAAAGCCCTT GACTTTTTTC AACCCTTTGT TTTCTGGGGC TAGCTGTGTT 
GGCCGCATGG ATAGGTTGGG CTATCCTCGC TGGACCAGGG GTCAGAACCG AGAGGGCGAG 
GAGTTCATCC GCAATGTCTT CCACCTTGTC ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC 
CAGCTCTGCT TCTCCTGGTT ACAGTATGAG ATTGCAAAGG TCATTTGGTG CCTGCACACT 
AAAAACAAGA AGAGATTAAA GTCTCAAGGG AAGAACTGCA AAAAACTAGC CAAGAATCTC 
CTTAAGGAGC CAGAAAACTG CAACAACTTT TGCCTGTGGA AGCAGTATGC ACATCTGGAG 
TGGTTGCTTG GCAACACGGA GGATGCCAGA AAAGTTTTTG ACACAGCACT TGGCATGGCA 
GGAAGCAGAG AACTGAAAGA CTCTGACCTC TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG 
GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG GCTGCCACAG CTCGAGCTGT TCACATATTA 
ACCAAGCTGA CTGAGAGCAG CCCCTATGGG CCCTACACTG GACAGGTGTT GGCTGTTCAC 
ATTTTGAAAG CGCGAAAGGC TTATGAGCAC GCACTGCAGG ACTGTTTGGG TGACAGCTGT 
GTCTCCAATC CAGCTCCCAC CGATTCCTGT AGCCGCCTAA TTAGCCTGGC TAAATGCTTC 
ATGCTCTTCC AGTATTTGAC CATAGGGATT GATGCTGCTG TGCAGATATA CGAACAGGTG 
TTTGCAAAAC TGAACAGTTC TGTTTTCCCA GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC 
TCCCAGAGTT GGACCAGTGT TCTCGAAGCC ATCACACTGA TGCACACGAG CCTGCTGAGA 
TTCCACATGA AAGTGAGTGT TTACCCGCTG GCCCCTCTGC GAGAGGCACT CTCACAGGCT 
TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT TGGAGGTCCT ATGTACAGAT TCAGAATAAG 
TCCCACAGTG CCAGCAAAAC CAGGAGATTT TTTGACACAA TCACCAGGTC TGCCAAACCC 
TTGGAGCCTT GGTTGTTTGC AATTGAAGCT GAGAAACTGA GGAAGAGACT GGTGGAAACT 
GTCCAGAGGT TAGACGGTAG AGAGATCCAC GCCACAATTC CTGAGACCGG CTTAATGCAT 
CGGATCCAAG CCCTGTTTGA AAATGCCATG CGCAGCGACA GTGGCAGCCA GTGCCCCTTG 
CTGTGGAGGA TGTATTTGAA CTTTCTGGTT TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT 
GTATTCTACA AAGCACTTCA GAATTGCCCT TGGGCAAAGG TGTTGTACCT GGACGCCGTG 
GAGTATTTCC CCGATGAGAT GCAGGAGATC CTGGACCTGA TGACTGAGAA GGAGCTCCGG 
GTGCGCCTGC CGCTGGAGGA GCTGGAGCTG CTGCTGGAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535188.1
CDS	32..3556
Translation	MALFPAFAGLSEAPDGGSSRKELDWLSNPSFCVGSITSLSQQTEAAPAHVSEGLPLTRSHLKSESSDESDTNKKLKQTSRKKKKEKKKKRKHQHHKKTKRKHGPSSSSRSETDTDSEKDKPSRGVGGSKKESEEPNQGNNAAADTGHRFVWLEDIQAVTGETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGTSTEKKHSRKQVERYFTKKSVGLMNIDGVAISSKTEPPSSEPISFIPVKDLEDAAPVTTWLNPLGIYDQSTTHWLQGQGPPEQESKQPDAQPDSESAALKAKVEEFNRRVRENPRDTQLWMAFVAFQDEVMKSPGLYAIEEGEQEKRKRSLKLILEKKLAILERAIESNQSSVDLKLAKLKLCTEFWEPSTLVKEWQKLIFLHPNNTALWQKYLLFCQSQFSTFSISKIHSLYGKCLSTLSAVKDGSILSHPALPGTEEAMFADSEHRMKVAALFLQQCHFLRQAGHSEKAISLFQAMVDFTFFKPDSVKDLPTKGQVEFFEPFWDSGEPRAGEKGARGWKAWMHQQERGGWVVINPDEDDDEPEEDDQEIKDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSHDLQFQLVEAFLQFLGVPSGFTPPASCLYLAMDENSIFDNGLYDEKPLTFFNPLFSGASCVGRMDRLGYPRWTRGQNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENCNNFCLWKQYAHLEWLLGNTEDARKVFDTALGMAGSRELKDSDLCELSLLYAELEVELSPEVRRAATARAVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKVLYLDAVEYFPDEMQEILDLMTEKELRVRLPLEELELLLED

Target ORF information:

RefSeq Version	XM_011536886.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536886.1

ORF Insert Sequence:

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCCGACTCTG AACATAGGAT GAAAGTTGCT 
GCACTCTTTC TTCAGCAGTG CCACTTTCTG CGGCAGGCTG GCCACTCTGA GAAGGCCATC 
TCATTGTTCC AGGCCATGGT GGACTTCACC TTCTTCAAAC CCGACAGCGT GAAAGATCTG 
CCTACCAAAG GACAGGTGGA ATTCTTTGAA CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT 
GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG TGGATGCACC AGCAGGAACG AGGTGGCTGG 
GTGGTCATCA ACCCAGATGA GGATGACGAT GAACCAGAAG AGGATGACCA GGAAATAAAA 
GATAAGACTC TGCCCAGGTG GCAGATCTGG CTTGCTGCTG AGCGTTCCCG TGACCAGAGG 
CACTGGCGGC CCTGGCGCCC TGATAAGACC AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT 
CCCGAGAGAC AGGTGTTGTT TGATGATATT GGGCAATCTT TGATCAGACT TTCCAGCCAT 
GATCTTCAGT TCCAGCTGGT GGAGGCCTTC CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT 
ACTCCTCCAG CCTCCTGTCT TTATCTGGCC ATGGATGAGA ACAGCATCTT TGATAATGGA 
CTTTATGATG AAAAGCCCTT GACTTTTTTC AACCCTTTGT TTTCTGGGGC TAGCTGTGTT 
GGCCGCATGG ATAGGTTGGG CTATCCTCGC TGGACCAGGG GTCAGAACCG AGAGGGCGAG 
GAGTTCATCC GCAATGTCTT CCACCTTGTC ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC 
CAGCTCTGCT TCTCCTGGTT ACAGTATGAG ATTGCAAAGG TCATTTGGTG CCTGCACACT 
AAAAACAAGA AGAGATTAAA GTCTCAAGGG AAGAACTGCA AAAAACTAGC CAAGAATCTC 
CTTAAGGAGC CAGAAAACTG CAACAACTTT TGCCTGTGGA AGCAGTATGC ACATCTGGAG 
TGGTTGCTTG GCAACACGGA GGATGCCAGA AAAGTTTTTG ACACAGCACT TGGCATGGCA 
GGAAGCAGAG AACTGAAAGA CTCTGACCTC TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG 
GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG GCTGCCACAG CTCGAGCTGT TCACATATTA 
ACCAAGCTGA CTGAGAGCAG CCCCTATGGG CCCTACACTG GACAGGTGTT GGCTGTTCAC 
ATTTTGAAAG CGCGAAAGGC TTATGAGCAC GCACTGCAGG ACTGTTTGGG TGACAGCTGT 
GTCTCCAATC CAGCTCCCAC CGATTCCTGT AGCCGCCTAA TTAGCCTGGC TAAATGCTTC 
ATGCTCTTCC AGTATTTGAC CATAGGGATT GATGCTGCTG TGCAGATATA CGAACAGGTG 
TTTGCAAAAC TGAACAGTTC TGTTTTCCCA GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC 
TCCCAGAGTT GGACCAGTGT TCTCGAAGCC ATCACACTGA TGCACACGAG CCTGCTGAGA 
TTCCACATGA AAGTGAGTGT TTACCCGCTG GCCCCTCTGC GAGAGGCACT CTCACAGGCT 
TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT TGGAGGTCCT ATGTACAGAT TCAGAATAAG 
TCCCACAGTG CCAGCAAAAC CAGGAGATTT TTTGACACAA TCACCAGGTC TGCCAAACCC 
TTGGAGCCTT GGTTGTTTGC AATTGAAGCT GAGAAACTGA GGAAGAGACT GGTGGAAACT 
GTCCAGAGGT TAGACGGTAG AGAGATCCAC GCCACAATTC CTGAGACCGG CTTAATGCAT 
CGGATCCAAG CCCTGTTTGA AAATGCCATG CGCAGCGACA GTGGCAGCCA GTGCCCCTTG 
CTGTGGAGGA TGTATTTGAA CTTTCTGGTT TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT 
GTATTCTACA AAGCACTTCA GAATTGCCCT TGGGCAAAGG TGTTGTACCT GGACGCCGTG 
GAGTATTTCC CCGATGAGAT GCAGGAGATC CTGGACCTGA TGACTGAGAA GGAGCTCCGG 
GTGCGCCTGC CGCTGGAGGA GCTGGAGCTG CTGCTGGAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRDE2 ( XM_017021394.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876883.1 Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X2, mRNA.

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CloneID	OHu84960
Clone ID Related Accession (Same CDS sequence)	XM_017021394.2 , XM_017021394.1
Accession Version	XM_017021394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	nuclear exosome regulator NRDE2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCCGACTCTG AACATAGGAT GAAAGTTGCT 
GCACTCTTTC TTCAGCAGTG CCACTTTCTG CGGCAGGCTG GCCACTCTGA GAAGGCCATC 
TCATTGTTCC AGGCCATGGT GGACTTCACC TTCTTCAAAC CCGACAGCGT GAAAGATCTG 
CCTACCAAAG GACAGGTGGA ATTCTTTGAA CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT 
GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG TGGATGCACC AGCAGGAACG AGGTGGCTGG 
GTGGTCATCA ACCCAGATGA GGATGACGAT GAACCAGAAG AGGATGACCA GGAAATAAAA 
GATAAGACTC TGCCCAGGTG GCAGATCTGG CTTGCTGCTG AGCGTTCCCG TGACCAGAGG 
CACTGGCGGC CCTGGCGCCC TGATAAGACC AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT 
CCCGAGAGAC AGGTGTTGTT TGATGATATT GGGCAATCTT TGATCAGACT TTCCAGCCAT 
GATCTTCAGT TCCAGCTGGT GGAGGCCTTC CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT 
ACTCCTCCAG CCTCCTGTCT TTATCTGGCC ATGGATGAGA ACAGCATCTT TGATAATGGA 
CTTTATGATG AAAAGCCCTT GACTTTTTTC AACCCTTTGT TTTCTGGGGC TAGCTGTGTT 
GGCCGCATGG ATAGGTTGGG CTATCCTCGC TGGACCAGGG GTCAGAACCG AGAGGGCGAG 
GAGTTCATCC GCAATGTCTT CCACCTTGTC ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC 
CAGCTCTGCT TCTCCTGGTT ACAGTATGAG ATTGCAAAGG TCATTTGGTG CCTGCACACT 
AAAAACAAGA AGAGATTAAA GTCTCAAGGG AAGAACTGCA AAAAACTAGC CAAGAATCTC 
CTTAAGGAGC CAGAAAACTG CAACAACTTT TGCCTGTGGA AGCAGTATGC ACATCTGGAG 
TGGTTGCTTG GCAACACGGA GGATGCCAGA AAAGTTTTTG ACACAGCACT TGGCATGGCA 
GGAAGCAGAG AACTGAAAGA CTCTGACCTC TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG 
GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG GCTGCCACAG CTCGAGCTGT TCACATATTA 
ACCAAGCTGA CTGAGAGCAG CCCCTATGGG CCCTACACTG GACAGGTGTT GGCTGTTCAC 
ATTTTGAAAG CGCGAAAGGC TTATGAGCAC GCACTGCAGG ACTGTTTGGG TGACAGCTGT 
GTCTCCAATC CAGCTCCCAC CGATTCCTGT AGCCGCCTAA TTAGCCTGGC TAAATGCTTC 
ATGCTCTTCC AGTATTTGAC CATAGGGATT GATGCTGCTG TGCAGATATA CGAACAGGTG 
TTTGCAAAAC TGAACAGTTC TGTTTTCCCA GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC 
TCCCAGAGTT GGACCAGTGT TCTCGAAGCC ATCACACTGA TGCACACGAG CCTGCTGAGA 
TTCCACATGA AAGTGAGTGT TTACCCGCTG GCCCCTCTGC GAGAGGCACT CTCACAGGCT 
TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT TGGAGGTCCT ATGTACAGAT TCAGAATAAG 
TCCCACAGTG CCAGCAAAAC CAGGAGATTT TTTGACACAA TCACCAGGTC TGCCAAACCC 
TTGGAGCCTT GGTTGTTTGC AATTGAAGCT GAGAAACTGA GGAAGAGACT GGTGGAAACT 
GTCCAGAGGT TAGACGGTAG AGAGATCCAC GCCACAATTC CTGAGACCGG CTTAATGCAT 
CGGATCCAAG CCCTGTTTGA AAATGCCATG CGCAGCGACA GTGGCAGCCA GTGCCCCTTG 
CTGTGGAGGA TGTATTTGAA CTTTCTGGTT TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT 
GTATTCTACA AAGCACTTCA GAATTGCCCT TGGGCAAAGG AAAAGATGCA AGTGCGTTAC 
AAAGAATTTT GTTTCCTGAA CCGTGTGAGT CAGTTGCTGA GAGTCTGCTA CCCGCCCACG 
ATACAGACAC TGAACAGGAA GTCAGCATGG ACGCAGTACT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876883.1
CDS	33..3557
Translation	MALFPAFAGLSEAPDGGSSRKELDWLSNPSFCVGSITSLSQQTEAAPAHVSEGLPLTRSHLKSESSDESDTNKKLKQTSRKKKKEKKKKRKHQHHKKTKRKHGPSSSSRSETDTDSEKDKPSRGVGGSKKESEEPNQGNNAAADTGHRFVWLEDIQAVTGETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGTSTEKKHSRKQVERYFTKKSVGLMNIDGVAISSKTEPPSSEPISFIPVKDLEDAAPVTTWLNPLGIYDQSTTHWLQGQGPPEQESKQPDAQPDSESAALKAKVEEFNRRVRENPRDTQLWMAFVAFQDEVMKSPGLYAIEEGEQEKRKRSLKLILEKKLAILERAIESNQSSVDLKLAKLKLCTEFWEPSTLVKEWQKLIFLHPNNTALWQKYLLFCQSQFSTFSISKIHSLYGKCLSTLSAVKDGSILSHPALPGTEEAMFADSEHRMKVAALFLQQCHFLRQAGHSEKAISLFQAMVDFTFFKPDSVKDLPTKGQVEFFEPFWDSGEPRAGEKGARGWKAWMHQQERGGWVVINPDEDDDEPEEDDQEIKDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSHDLQFQLVEAFLQFLGVPSGFTPPASCLYLAMDENSIFDNGLYDEKPLTFFNPLFSGASCVGRMDRLGYPRWTRGQNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENCNNFCLWKQYAHLEWLLGNTEDARKVFDTALGMAGSRELKDSDLCELSLLYAELEVELSPEVRRAATARAVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKEKMQVRYKEFCFLNRVSQLLRVCYPPTIQTLNRKSAWTQYS

Target ORF information:

RefSeq Version	XM_017021394.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021394.2

ORF Insert Sequence:

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCCGACTCTG AACATAGGAT GAAAGTTGCT 
GCACTCTTTC TTCAGCAGTG CCACTTTCTG CGGCAGGCTG GCCACTCTGA GAAGGCCATC 
TCATTGTTCC AGGCCATGGT GGACTTCACC TTCTTCAAAC CCGACAGCGT GAAAGATCTG 
CCTACCAAAG GACAGGTGGA ATTCTTTGAA CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT 
GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG TGGATGCACC AGCAGGAACG AGGTGGCTGG 
GTGGTCATCA ACCCAGATGA GGATGACGAT GAACCAGAAG AGGATGACCA GGAAATAAAA 
GATAAGACTC TGCCCAGGTG GCAGATCTGG CTTGCTGCTG AGCGTTCCCG TGACCAGAGG 
CACTGGCGGC CCTGGCGCCC TGATAAGACC AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT 
CCCGAGAGAC AGGTGTTGTT TGATGATATT GGGCAATCTT TGATCAGACT TTCCAGCCAT 
GATCTTCAGT TCCAGCTGGT GGAGGCCTTC CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT 
ACTCCTCCAG CCTCCTGTCT TTATCTGGCC ATGGATGAGA ACAGCATCTT TGATAATGGA 
CTTTATGATG AAAAGCCCTT GACTTTTTTC AACCCTTTGT TTTCTGGGGC TAGCTGTGTT 
GGCCGCATGG ATAGGTTGGG CTATCCTCGC TGGACCAGGG GTCAGAACCG AGAGGGCGAG 
GAGTTCATCC GCAATGTCTT CCACCTTGTC ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC 
CAGCTCTGCT TCTCCTGGTT ACAGTATGAG ATTGCAAAGG TCATTTGGTG CCTGCACACT 
AAAAACAAGA AGAGATTAAA GTCTCAAGGG AAGAACTGCA AAAAACTAGC CAAGAATCTC 
CTTAAGGAGC CAGAAAACTG CAACAACTTT TGCCTGTGGA AGCAGTATGC ACATCTGGAG 
TGGTTGCTTG GCAACACGGA GGATGCCAGA AAAGTTTTTG ACACAGCACT TGGCATGGCA 
GGAAGCAGAG AACTGAAAGA CTCTGACCTC TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG 
GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG GCTGCCACAG CTCGAGCTGT TCACATATTA 
ACCAAGCTGA CTGAGAGCAG CCCCTATGGG CCCTACACTG GACAGGTGTT GGCTGTTCAC 
ATTTTGAAAG CGCGAAAGGC TTATGAGCAC GCACTGCAGG ACTGTTTGGG TGACAGCTGT 
GTCTCCAATC CAGCTCCCAC CGATTCCTGT AGCCGCCTAA TTAGCCTGGC TAAATGCTTC 
ATGCTCTTCC AGTATTTGAC CATAGGGATT GATGCTGCTG TGCAGATATA CGAACAGGTG 
TTTGCAAAAC TGAACAGTTC TGTTTTCCCA GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC 
TCCCAGAGTT GGACCAGTGT TCTCGAAGCC ATCACACTGA TGCACACGAG CCTGCTGAGA 
TTCCACATGA AAGTGAGTGT TTACCCGCTG GCCCCTCTGC GAGAGGCACT CTCACAGGCT 
TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT TGGAGGTCCT ATGTACAGAT TCAGAATAAG 
TCCCACAGTG CCAGCAAAAC CAGGAGATTT TTTGACACAA TCACCAGGTC TGCCAAACCC 
TTGGAGCCTT GGTTGTTTGC AATTGAAGCT GAGAAACTGA GGAAGAGACT GGTGGAAACT 
GTCCAGAGGT TAGACGGTAG AGAGATCCAC GCCACAATTC CTGAGACCGG CTTAATGCAT 
CGGATCCAAG CCCTGTTTGA AAATGCCATG CGCAGCGACA GTGGCAGCCA GTGCCCCTTG 
CTGTGGAGGA TGTATTTGAA CTTTCTGGTT TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT 
GTATTCTACA AAGCACTTCA GAATTGCCCT TGGGCAAAGG AAAAGATGCA AGTGCGTTAC 
AAAGAATTTT GTTTCCTGAA CCGTGTGAGT CAGTTGCTGA GAGTCTGCTA CCCGCCCACG 
ATACAGACAC TGAACAGGAA GTCAGCATGG ACGCAGTACT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRDE2 ( XM_017021394.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876883.1 Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X2, mRNA.

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CloneID	OHu84960
Clone ID Related Accession (Same CDS sequence)	XM_017021394.2 , XM_017021394.1
Accession Version	XM_017021394.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	protein NRDE2 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCCGACTCTG AACATAGGAT GAAAGTTGCT 
GCACTCTTTC TTCAGCAGTG CCACTTTCTG CGGCAGGCTG GCCACTCTGA GAAGGCCATC 
TCATTGTTCC AGGCCATGGT GGACTTCACC TTCTTCAAAC CCGACAGCGT GAAAGATCTG 
CCTACCAAAG GACAGGTGGA ATTCTTTGAA CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT 
GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG TGGATGCACC AGCAGGAACG AGGTGGCTGG 
GTGGTCATCA ACCCAGATGA GGATGACGAT GAACCAGAAG AGGATGACCA GGAAATAAAA 
GATAAGACTC TGCCCAGGTG GCAGATCTGG CTTGCTGCTG AGCGTTCCCG TGACCAGAGG 
CACTGGCGGC CCTGGCGCCC TGATAAGACC AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT 
CCCGAGAGAC AGGTGTTGTT TGATGATATT GGGCAATCTT TGATCAGACT TTCCAGCCAT 
GATCTTCAGT TCCAGCTGGT GGAGGCCTTC CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT 
ACTCCTCCAG CCTCCTGTCT TTATCTGGCC ATGGATGAGA ACAGCATCTT TGATAATGGA 
CTTTATGATG AAAAGCCCTT GACTTTTTTC AACCCTTTGT TTTCTGGGGC TAGCTGTGTT 
GGCCGCATGG ATAGGTTGGG CTATCCTCGC TGGACCAGGG GTCAGAACCG AGAGGGCGAG 
GAGTTCATCC GCAATGTCTT CCACCTTGTC ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC 
CAGCTCTGCT TCTCCTGGTT ACAGTATGAG ATTGCAAAGG TCATTTGGTG CCTGCACACT 
AAAAACAAGA AGAGATTAAA GTCTCAAGGG AAGAACTGCA AAAAACTAGC CAAGAATCTC 
CTTAAGGAGC CAGAAAACTG CAACAACTTT TGCCTGTGGA AGCAGTATGC ACATCTGGAG 
TGGTTGCTTG GCAACACGGA GGATGCCAGA AAAGTTTTTG ACACAGCACT TGGCATGGCA 
GGAAGCAGAG AACTGAAAGA CTCTGACCTC TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG 
GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG GCTGCCACAG CTCGAGCTGT TCACATATTA 
ACCAAGCTGA CTGAGAGCAG CCCCTATGGG CCCTACACTG GACAGGTGTT GGCTGTTCAC 
ATTTTGAAAG CGCGAAAGGC TTATGAGCAC GCACTGCAGG ACTGTTTGGG TGACAGCTGT 
GTCTCCAATC CAGCTCCCAC CGATTCCTGT AGCCGCCTAA TTAGCCTGGC TAAATGCTTC 
ATGCTCTTCC AGTATTTGAC CATAGGGATT GATGCTGCTG TGCAGATATA CGAACAGGTG 
TTTGCAAAAC TGAACAGTTC TGTTTTCCCA GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC 
TCCCAGAGTT GGACCAGTGT TCTCGAAGCC ATCACACTGA TGCACACGAG CCTGCTGAGA 
TTCCACATGA AAGTGAGTGT TTACCCGCTG GCCCCTCTGC GAGAGGCACT CTCACAGGCT 
TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT TGGAGGTCCT ATGTACAGAT TCAGAATAAG 
TCCCACAGTG CCAGCAAAAC CAGGAGATTT TTTGACACAA TCACCAGGTC TGCCAAACCC 
TTGGAGCCTT GGTTGTTTGC AATTGAAGCT GAGAAACTGA GGAAGAGACT GGTGGAAACT 
GTCCAGAGGT TAGACGGTAG AGAGATCCAC GCCACAATTC CTGAGACCGG CTTAATGCAT 
CGGATCCAAG CCCTGTTTGA AAATGCCATG CGCAGCGACA GTGGCAGCCA GTGCCCCTTG 
CTGTGGAGGA TGTATTTGAA CTTTCTGGTT TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT 
GTATTCTACA AAGCACTTCA GAATTGCCCT TGGGCAAAGG AAAAGATGCA AGTGCGTTAC 
AAAGAATTTT GTTTCCTGAA CCGTGTGAGT CAGTTGCTGA GAGTCTGCTA CCCGCCCACG 
ATACAGACAC TGAACAGGAA GTCAGCATGG ACGCAGTACT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876883.1
CDS	32..3556
Translation	MALFPAFAGLSEAPDGGSSRKELDWLSNPSFCVGSITSLSQQTEAAPAHVSEGLPLTRSHLKSESSDESDTNKKLKQTSRKKKKEKKKKRKHQHHKKTKRKHGPSSSSRSETDTDSEKDKPSRGVGGSKKESEEPNQGNNAAADTGHRFVWLEDIQAVTGETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGTSTEKKHSRKQVERYFTKKSVGLMNIDGVAISSKTEPPSSEPISFIPVKDLEDAAPVTTWLNPLGIYDQSTTHWLQGQGPPEQESKQPDAQPDSESAALKAKVEEFNRRVRENPRDTQLWMAFVAFQDEVMKSPGLYAIEEGEQEKRKRSLKLILEKKLAILERAIESNQSSVDLKLAKLKLCTEFWEPSTLVKEWQKLIFLHPNNTALWQKYLLFCQSQFSTFSISKIHSLYGKCLSTLSAVKDGSILSHPALPGTEEAMFADSEHRMKVAALFLQQCHFLRQAGHSEKAISLFQAMVDFTFFKPDSVKDLPTKGQVEFFEPFWDSGEPRAGEKGARGWKAWMHQQERGGWVVINPDEDDDEPEEDDQEIKDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSHDLQFQLVEAFLQFLGVPSGFTPPASCLYLAMDENSIFDNGLYDEKPLTFFNPLFSGASCVGRMDRLGYPRWTRGQNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENCNNFCLWKQYAHLEWLLGNTEDARKVFDTALGMAGSRELKDSDLCELSLLYAELEVELSPEVRRAATARAVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKEKMQVRYKEFCFLNRVSQLLRVCYPPTIQTLNRKSAWTQYS

Target ORF information:

RefSeq Version	XM_017021394.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021394.1

ORF Insert Sequence:

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCCGACTCTG AACATAGGAT GAAAGTTGCT 
GCACTCTTTC TTCAGCAGTG CCACTTTCTG CGGCAGGCTG GCCACTCTGA GAAGGCCATC 
TCATTGTTCC AGGCCATGGT GGACTTCACC TTCTTCAAAC CCGACAGCGT GAAAGATCTG 
CCTACCAAAG GACAGGTGGA ATTCTTTGAA CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT 
GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG TGGATGCACC AGCAGGAACG AGGTGGCTGG 
GTGGTCATCA ACCCAGATGA GGATGACGAT GAACCAGAAG AGGATGACCA GGAAATAAAA 
GATAAGACTC TGCCCAGGTG GCAGATCTGG CTTGCTGCTG AGCGTTCCCG TGACCAGAGG 
CACTGGCGGC CCTGGCGCCC TGATAAGACC AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT 
CCCGAGAGAC AGGTGTTGTT TGATGATATT GGGCAATCTT TGATCAGACT TTCCAGCCAT 
GATCTTCAGT TCCAGCTGGT GGAGGCCTTC CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT 
ACTCCTCCAG CCTCCTGTCT TTATCTGGCC ATGGATGAGA ACAGCATCTT TGATAATGGA 
CTTTATGATG AAAAGCCCTT GACTTTTTTC AACCCTTTGT TTTCTGGGGC TAGCTGTGTT 
GGCCGCATGG ATAGGTTGGG CTATCCTCGC TGGACCAGGG GTCAGAACCG AGAGGGCGAG 
GAGTTCATCC GCAATGTCTT CCACCTTGTC ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC 
CAGCTCTGCT TCTCCTGGTT ACAGTATGAG ATTGCAAAGG TCATTTGGTG CCTGCACACT 
AAAAACAAGA AGAGATTAAA GTCTCAAGGG AAGAACTGCA AAAAACTAGC CAAGAATCTC 
CTTAAGGAGC CAGAAAACTG CAACAACTTT TGCCTGTGGA AGCAGTATGC ACATCTGGAG 
TGGTTGCTTG GCAACACGGA GGATGCCAGA AAAGTTTTTG ACACAGCACT TGGCATGGCA 
GGAAGCAGAG AACTGAAAGA CTCTGACCTC TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG 
GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG GCTGCCACAG CTCGAGCTGT TCACATATTA 
ACCAAGCTGA CTGAGAGCAG CCCCTATGGG CCCTACACTG GACAGGTGTT GGCTGTTCAC 
ATTTTGAAAG CGCGAAAGGC TTATGAGCAC GCACTGCAGG ACTGTTTGGG TGACAGCTGT 
GTCTCCAATC CAGCTCCCAC CGATTCCTGT AGCCGCCTAA TTAGCCTGGC TAAATGCTTC 
ATGCTCTTCC AGTATTTGAC CATAGGGATT GATGCTGCTG TGCAGATATA CGAACAGGTG 
TTTGCAAAAC TGAACAGTTC TGTTTTCCCA GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC 
TCCCAGAGTT GGACCAGTGT TCTCGAAGCC ATCACACTGA TGCACACGAG CCTGCTGAGA 
TTCCACATGA AAGTGAGTGT TTACCCGCTG GCCCCTCTGC GAGAGGCACT CTCACAGGCT 
TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT TGGAGGTCCT ATGTACAGAT TCAGAATAAG 
TCCCACAGTG CCAGCAAAAC CAGGAGATTT TTTGACACAA TCACCAGGTC TGCCAAACCC 
TTGGAGCCTT GGTTGTTTGC AATTGAAGCT GAGAAACTGA GGAAGAGACT GGTGGAAACT 
GTCCAGAGGT TAGACGGTAG AGAGATCCAC GCCACAATTC CTGAGACCGG CTTAATGCAT 
CGGATCCAAG CCCTGTTTGA AAATGCCATG CGCAGCGACA GTGGCAGCCA GTGCCCCTTG 
CTGTGGAGGA TGTATTTGAA CTTTCTGGTT TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT 
GTATTCTACA AAGCACTTCA GAATTGCCCT TGGGCAAAGG AAAAGATGCA AGTGCGTTAC 
AAAGAATTTT GTTTCCTGAA CCGTGTGAGT CAGTTGCTGA GAGTCTGCTA CCCGCCCACG 
ATACAGACAC TGAACAGGAA GTCAGCATGG ACGCAGTACT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRDE2 ( NM_017970.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060440.2 Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.

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CloneID	OHu02637
Clone ID Related Accession (Same CDS sequence)	NM_017970.4 , NM_017970.3
Accession Version	NM_017970.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	nuclear exosome regulator NRDE2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK294958.1, BC008925.2, AW196499.1, AL161662.6 and AL355074.5. On Nov 22, 2018 this sequence version replaced NM_017970.3. ##Evidence-Data-START## Transcript exon combination :: AK294958.1, SRR1803616.261501.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000354366.8/ ENSP00000346335.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCACTCTTTC TTCAGCAGTG CCACTTTCTG 
CGGCAGGCTG GCCACTCTGA GAAGGCCATC TCATTGTTCC AGGCCATGGT GGACTTCACC 
TTCTTCAAAC CCGACAGCGT GAAAGATCTG CCTACCAAAG GACAGGTGGA ATTCTTTGAA 
CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG 
TGGATGCACC AGCAGGAACG AGGTGGCTGG GTGGTCATCA ACCCAGATGA GGATGACGAT 
GAACCAGAAG AGGATGACCA GGAAATAAAA GATAAGACTC TGCCCAGGTG GCAGATCTGG 
CTTGCTGCTG AGCGTTCCCG TGACCAGAGG CACTGGCGGC CCTGGCGCCC TGATAAGACC 
AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT CCCGAGAGAC AGGTGTTGTT TGATGATATT 
GGGCAATCTT TGATCAGACT TTCCAGCCAT GATCTTCAGT TCCAGCTGGT GGAGGCCTTC 
CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT ACTCCTCCAG CCTCCTGTCT TTATCTGGCC 
ATGGATGAGA ACAGCATCTT TGATAATGGA CTTTATGATG AAAAGCCCTT GACTTTTTTC 
AACCCTTTGT TTTCTGGGGC TAGCTGTGTT GGCCGCATGG ATAGGTTGGG CTATCCTCGC 
TGGACCAGGG GTCAGAACCG AGAGGGCGAG GAGTTCATCC GCAATGTCTT CCACCTTGTC 
ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC CAGCTCTGCT TCTCCTGGTT ACAGTATGAG 
ATTGCAAAGG TCATTTGGTG CCTGCACACT AAAAACAAGA AGAGATTAAA GTCTCAAGGG 
AAGAACTGCA AAAAACTAGC CAAGAATCTC CTTAAGGAGC CAGAAAACTG CAACAACTTT 
TGCCTGTGGA AGCAGTATGC ACATCTGGAG TGGTTGCTTG GCAACACGGA GGATGCCAGA 
AAAGTTTTTG ACACAGCACT TGGCATGGCA GGAAGCAGAG AACTGAAAGA CTCTGACCTC 
TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG 
GCTGCCACAG CTCGAGCTGT TCACATATTA ACCAAGCTGA CTGAGAGCAG CCCCTATGGG 
CCCTACACTG GACAGGTGTT GGCTGTTCAC ATTTTGAAAG CGCGAAAGGC TTATGAGCAC 
GCACTGCAGG ACTGTTTGGG TGACAGCTGT GTCTCCAATC CAGCTCCCAC CGATTCCTGT 
AGCCGCCTAA TTAGCCTGGC TAAATGCTTC ATGCTCTTCC AGTATTTGAC CATAGGGATT 
GATGCTGCTG TGCAGATATA CGAACAGGTG TTTGCAAAAC TGAACAGTTC TGTTTTCCCA 
GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC TCCCAGAGTT GGACCAGTGT TCTCGAAGCC 
ATCACACTGA TGCACACGAG CCTGCTGAGA TTCCACATGA AAGTGAGTGT TTACCCGCTG 
GCCCCTCTGC GAGAGGCACT CTCACAGGCT TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT 
TGGAGGTCCT ATGTACAGAT TCAGAATAAG TCCCACAGTG CCAGCAAAAC CAGGAGATTT 
TTTGACACAA TCACCAGGTC TGCCAAACCC TTGGAGCCTT GGTTGTTTGC AATTGAAGCT 
GAGAAACTGA GGAAGAGACT GGTGGAAACT GTCCAGAGGT TAGACGGTAG AGAGATCCAC 
GCCACAATTC CTGAGACCGG CTTAATGCAT CGGATCCAAG CCCTGTTTGA AAATGCCATG 
CGCAGCGACA GTGGCAGCCA GTGCCCCTTG CTGTGGAGGA TGTATTTGAA CTTTCTGGTT 
TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT GTATTCTACA AAGCACTTCA GAATTGCCCT 
TGGGCAAAGG TGTTGTACCT GGACGCCGTG GAGTATTTCC CCGATGAGAT GCAGGAGATC 
CTGGACCTGA TGACTGAGAA GGAGCTCCGG GTGCGCCTGC CGCTGGAGGA GCTGGAGCTG 
CTGCTGGAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060440.2
CDS	38..3532
Translation	MALFPAFAGLSEAPDGGSSRKELDWLSNPSFCVGSITSLSQQTEAAPAHVSEGLPLTRSHLKSESSDESDTNKKLKQTSRKKKKEKKKKRKHQHHKKTKRKHGPSSSSRSETDTDSEKDKPSRGVGGSKKESEEPNQGNNAAADTGHRFVWLEDIQAVTGETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGTSTEKKHSRKQVERYFTKKSVGLMNIDGVAISSKTEPPSSEPISFIPVKDLEDAAPVTTWLNPLGIYDQSTTHWLQGQGPPEQESKQPDAQPDSESAALKAKVEEFNRRVRENPRDTQLWMAFVAFQDEVMKSPGLYAIEEGEQEKRKRSLKLILEKKLAILERAIESNQSSVDLKLAKLKLCTEFWEPSTLVKEWQKLIFLHPNNTALWQKYLLFCQSQFSTFSISKIHSLYGKCLSTLSAVKDGSILSHPALPGTEEAMFALFLQQCHFLRQAGHSEKAISLFQAMVDFTFFKPDSVKDLPTKGQVEFFEPFWDSGEPRAGEKGARGWKAWMHQQERGGWVVINPDEDDDEPEEDDQEIKDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSHDLQFQLVEAFLQFLGVPSGFTPPASCLYLAMDENSIFDNGLYDEKPLTFFNPLFSGASCVGRMDRLGYPRWTRGQNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENCNNFCLWKQYAHLEWLLGNTEDARKVFDTALGMAGSRELKDSDLCELSLLYAELEVELSPEVRRAATARAVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKVLYLDAVEYFPDEMQEILDLMTEKELRVRLPLEELELLLED

Target ORF information:

RefSeq Version	NM_017970.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017970.4

ORF Insert Sequence:

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCACTCTTTC TTCAGCAGTG CCACTTTCTG 
CGGCAGGCTG GCCACTCTGA GAAGGCCATC TCATTGTTCC AGGCCATGGT GGACTTCACC 
TTCTTCAAAC CCGACAGCGT GAAAGATCTG CCTACCAAAG GACAGGTGGA ATTCTTTGAA 
CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG 
TGGATGCACC AGCAGGAACG AGGTGGCTGG GTGGTCATCA ACCCAGATGA GGATGACGAT 
GAACCAGAAG AGGATGACCA GGAAATAAAA GATAAGACTC TGCCCAGGTG GCAGATCTGG 
CTTGCTGCTG AGCGTTCCCG TGACCAGAGG CACTGGCGGC CCTGGCGCCC TGATAAGACC 
AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT CCCGAGAGAC AGGTGTTGTT TGATGATATT 
GGGCAATCTT TGATCAGACT TTCCAGCCAT GATCTTCAGT TCCAGCTGGT GGAGGCCTTC 
CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT ACTCCTCCAG CCTCCTGTCT TTATCTGGCC 
ATGGATGAGA ACAGCATCTT TGATAATGGA CTTTATGATG AAAAGCCCTT GACTTTTTTC 
AACCCTTTGT TTTCTGGGGC TAGCTGTGTT GGCCGCATGG ATAGGTTGGG CTATCCTCGC 
TGGACCAGGG GTCAGAACCG AGAGGGCGAG GAGTTCATCC GCAATGTCTT CCACCTTGTC 
ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC CAGCTCTGCT TCTCCTGGTT ACAGTATGAG 
ATTGCAAAGG TCATTTGGTG CCTGCACACT AAAAACAAGA AGAGATTAAA GTCTCAAGGG 
AAGAACTGCA AAAAACTAGC CAAGAATCTC CTTAAGGAGC CAGAAAACTG CAACAACTTT 
TGCCTGTGGA AGCAGTATGC ACATCTGGAG TGGTTGCTTG GCAACACGGA GGATGCCAGA 
AAAGTTTTTG ACACAGCACT TGGCATGGCA GGAAGCAGAG AACTGAAAGA CTCTGACCTC 
TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG 
GCTGCCACAG CTCGAGCTGT TCACATATTA ACCAAGCTGA CTGAGAGCAG CCCCTATGGG 
CCCTACACTG GACAGGTGTT GGCTGTTCAC ATTTTGAAAG CGCGAAAGGC TTATGAGCAC 
GCACTGCAGG ACTGTTTGGG TGACAGCTGT GTCTCCAATC CAGCTCCCAC CGATTCCTGT 
AGCCGCCTAA TTAGCCTGGC TAAATGCTTC ATGCTCTTCC AGTATTTGAC CATAGGGATT 
GATGCTGCTG TGCAGATATA CGAACAGGTG TTTGCAAAAC TGAACAGTTC TGTTTTCCCA 
GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC TCCCAGAGTT GGACCAGTGT TCTCGAAGCC 
ATCACACTGA TGCACACGAG CCTGCTGAGA TTCCACATGA AAGTGAGTGT TTACCCGCTG 
GCCCCTCTGC GAGAGGCACT CTCACAGGCT TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT 
TGGAGGTCCT ATGTACAGAT TCAGAATAAG TCCCACAGTG CCAGCAAAAC CAGGAGATTT 
TTTGACACAA TCACCAGGTC TGCCAAACCC TTGGAGCCTT GGTTGTTTGC AATTGAAGCT 
GAGAAACTGA GGAAGAGACT GGTGGAAACT GTCCAGAGGT TAGACGGTAG AGAGATCCAC 
GCCACAATTC CTGAGACCGG CTTAATGCAT CGGATCCAAG CCCTGTTTGA AAATGCCATG 
CGCAGCGACA GTGGCAGCCA GTGCCCCTTG CTGTGGAGGA TGTATTTGAA CTTTCTGGTT 
TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT GTATTCTACA AAGCACTTCA GAATTGCCCT 
TGGGCAAAGG TGTTGTACCT GGACGCCGTG GAGTATTTCC CCGATGAGAT GCAGGAGATC 
CTGGACCTGA TGACTGAGAA GGAGCTCCGG GTGCGCCTGC CGCTGGAGGA GCTGGAGCTG 
CTGCTGGAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRDE2 ( NM_017970.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060440.2 Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.

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CloneID	OHu02637
Clone ID Related Accession (Same CDS sequence)	NM_017970.4 , NM_017970.3
Accession Version	NM_017970.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506787200000
Organism	Homo sapiens(human)
Product	protein NRDE2 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC393057.1, AK294958.1, BC008925.2 and AW196499.1. On Jul 27, 2011 this sequence version replaced NM_017970.2. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.252821.1, SRR1803617.97652.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCACTCTTTC TTCAGCAGTG CCACTTTCTG 
CGGCAGGCTG GCCACTCTGA GAAGGCCATC TCATTGTTCC AGGCCATGGT GGACTTCACC 
TTCTTCAAAC CCGACAGCGT GAAAGATCTG CCTACCAAAG GACAGGTGGA ATTCTTTGAA 
CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG 
TGGATGCACC AGCAGGAACG AGGTGGCTGG GTGGTCATCA ACCCAGATGA GGATGACGAT 
GAACCAGAAG AGGATGACCA GGAAATAAAA GATAAGACTC TGCCCAGGTG GCAGATCTGG 
CTTGCTGCTG AGCGTTCCCG TGACCAGAGG CACTGGCGGC CCTGGCGCCC TGATAAGACC 
AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT CCCGAGAGAC AGGTGTTGTT TGATGATATT 
GGGCAATCTT TGATCAGACT TTCCAGCCAT GATCTTCAGT TCCAGCTGGT GGAGGCCTTC 
CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT ACTCCTCCAG CCTCCTGTCT TTATCTGGCC 
ATGGATGAGA ACAGCATCTT TGATAATGGA CTTTATGATG AAAAGCCCTT GACTTTTTTC 
AACCCTTTGT TTTCTGGGGC TAGCTGTGTT GGCCGCATGG ATAGGTTGGG CTATCCTCGC 
TGGACCAGGG GTCAGAACCG AGAGGGCGAG GAGTTCATCC GCAATGTCTT CCACCTTGTC 
ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC CAGCTCTGCT TCTCCTGGTT ACAGTATGAG 
ATTGCAAAGG TCATTTGGTG CCTGCACACT AAAAACAAGA AGAGATTAAA GTCTCAAGGG 
AAGAACTGCA AAAAACTAGC CAAGAATCTC CTTAAGGAGC CAGAAAACTG CAACAACTTT 
TGCCTGTGGA AGCAGTATGC ACATCTGGAG TGGTTGCTTG GCAACACGGA GGATGCCAGA 
AAAGTTTTTG ACACAGCACT TGGCATGGCA GGAAGCAGAG AACTGAAAGA CTCTGACCTC 
TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG 
GCTGCCACAG CTCGAGCTGT TCACATATTA ACCAAGCTGA CTGAGAGCAG CCCCTATGGG 
CCCTACACTG GACAGGTGTT GGCTGTTCAC ATTTTGAAAG CGCGAAAGGC TTATGAGCAC 
GCACTGCAGG ACTGTTTGGG TGACAGCTGT GTCTCCAATC CAGCTCCCAC CGATTCCTGT 
AGCCGCCTAA TTAGCCTGGC TAAATGCTTC ATGCTCTTCC AGTATTTGAC CATAGGGATT 
GATGCTGCTG TGCAGATATA CGAACAGGTG TTTGCAAAAC TGAACAGTTC TGTTTTCCCA 
GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC TCCCAGAGTT GGACCAGTGT TCTCGAAGCC 
ATCACACTGA TGCACACGAG CCTGCTGAGA TTCCACATGA AAGTGAGTGT TTACCCGCTG 
GCCCCTCTGC GAGAGGCACT CTCACAGGCT TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT 
TGGAGGTCCT ATGTACAGAT TCAGAATAAG TCCCACAGTG CCAGCAAAAC CAGGAGATTT 
TTTGACACAA TCACCAGGTC TGCCAAACCC TTGGAGCCTT GGTTGTTTGC AATTGAAGCT 
GAGAAACTGA GGAAGAGACT GGTGGAAACT GTCCAGAGGT TAGACGGTAG AGAGATCCAC 
GCCACAATTC CTGAGACCGG CTTAATGCAT CGGATCCAAG CCCTGTTTGA AAATGCCATG 
CGCAGCGACA GTGGCAGCCA GTGCCCCTTG CTGTGGAGGA TGTATTTGAA CTTTCTGGTT 
TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT GTATTCTACA AAGCACTTCA GAATTGCCCT 
TGGGCAAAGG TGTTGTACCT GGACGCCGTG GAGTATTTCC CCGATGAGAT GCAGGAGATC 
CTGGACCTGA TGACTGAGAA GGAGCTCCGG GTGCGCCTGC CGCTGGAGGA GCTGGAGCTG 
CTGCTGGAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060440.2
CDS	234..3728
Translation	MALFPAFAGLSEAPDGGSSRKELDWLSNPSFCVGSITSLSQQTEAAPAHVSEGLPLTRSHLKSESSDESDTNKKLKQTSRKKKKEKKKKRKHQHHKKTKRKHGPSSSSRSETDTDSEKDKPSRGVGGSKKESEEPNQGNNAAADTGHRFVWLEDIQAVTGETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGTSTEKKHSRKQVERYFTKKSVGLMNIDGVAISSKTEPPSSEPISFIPVKDLEDAAPVTTWLNPLGIYDQSTTHWLQGQGPPEQESKQPDAQPDSESAALKAKVEEFNRRVRENPRDTQLWMAFVAFQDEVMKSPGLYAIEEGEQEKRKRSLKLILEKKLAILERAIESNQSSVDLKLAKLKLCTEFWEPSTLVKEWQKLIFLHPNNTALWQKYLLFCQSQFSTFSISKIHSLYGKCLSTLSAVKDGSILSHPALPGTEEAMFALFLQQCHFLRQAGHSEKAISLFQAMVDFTFFKPDSVKDLPTKGQVEFFEPFWDSGEPRAGEKGARGWKAWMHQQERGGWVVINPDEDDDEPEEDDQEIKDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSLIRLSSHDLQFQLVEAFLQFLGVPSGFTPPASCLYLAMDENSIFDNGLYDEKPLTFFNPLFSGASCVGRMDRLGYPRWTRGQNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLQYEIAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENCNNFCLWKQYAHLEWLLGNTEDARKVFDTALGMAGSRELKDSDLCELSLLYAELEVELSPEVRRAATARAVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKVLYLDAVEYFPDEMQEILDLMTEKELRVRLPLEELELLLED

Target ORF information:

RefSeq Version	NM_017970.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017970.3

ORF Insert Sequence:

ATGGCGCTGT TCCCAGCCTT TGCGGGGCTT AGTGAGGCTC CCGATGGCGG GAGCTCCAGG 
AAAGAGTTAG ACTGGCTGAG CAACCCAAGC TTTTGTGTTG GATCCATAAC GTCCCTGAGC 
CAACAAACTG AAGCAGCTCC AGCCCATGTT TCTGAAGGGT TACCGCTGAC AAGGAGTCAT 
CTGAAATCAG AGTCTTCAGA TGAAAGTGAC ACTAACAAAA AGCTCAAACA AACAAGTAGA 
AAAAAGAAGA AAGAGAAAAA GAAAAAAAGG AAGCATCAGC ATCATAAGAA AACAAAGAGG 
AAGCATGGGC CGTCGAGTAG CAGCAGGTCT GAGACAGACA CCGATTCTGA AAAGGACAAA 
CCTTCCAGAG GCGTTGGAGG CAGTAAAAAG GAATCTGAGG AACCGAATCA AGGAAATAAT 
GCTGCAGCTG ATACTGGACA TCGCTTTGTT TGGCTTGAGG ACATTCAGGC TGTGACGGGA 
GAAACCTTCA GAACAGATAA GAAACCAGAT CCTGCGAACT GGGAGTACAA GTCTCTCTAC 
CGAGGGGATA TAGCAAGATA CAAGAGGAAA GGAGACTCCT GCCTTGGCAT TAACCCTAAG 
AAGCAGTGCA TATCTTGGGA AGGGACTTCC ACAGAGAAGA AGCATTCACG CAAGCAGGTT 
GAACGCTATT TTACTAAGAA GAGTGTGGGA TTAATGAACA TCGATGGAGT TGCCATTAGC 
AGTAAAACTG AACCTCCCTC ATCTGAGCCC ATCTCCTTTA TACCAGTGAA GGACTTGGAA 
GATGCGGCTC CTGTTACAAC CTGGTTGAAT CCTCTGGGGA TTTATGATCA GTCAACCACA 
CATTGGCTAC AAGGACAGGG TCCTCCAGAG CAGGAATCAA AGCAGCCAGA CGCACAGCCA 
GACAGCGAGA GTGCGGCTCT CAAGGCCAAG GTGGAGGAGT TTAACAGGAG GGTGCGGGAG 
AATCCTCGGG ATACGCAGCT GTGGATGGCA TTTGTTGCTT TTCAGGACGA GGTCATGAAA 
AGTCCTGGCC TGTATGCCAT CGAGGAAGGA GAGCAGGAAA AGCGAAAGAG GTCCCTGAAG 
CTCATTCTGG AGAAGAAGCT GGCCATTCTG GAGCGGGCCA TTGAGAGCAA CCAGAGCAGT 
GTGGATCTGA AACTGGCCAA GCTGAAGCTC TGCACAGAGT TCTGGGAGCC CTCCACTCTG 
GTCAAAGAGT GGCAGAAACT GATATTTTTG CATCCCAATA ATACAGCCCT TTGGCAGAAA 
TACCTTTTAT TTTGCCAGAG CCAGTTTAGT ACCTTTTCGA TATCAAAAAT TCACAGTCTT 
TATGGAAAAT GCTTGAGCAC TTTGTCTGCT GTTAAGGACG GCAGCATCTT ATCTCACCCT 
GCGTTGCCTG GCACGGAAGA GGCCATGTTT GCACTCTTTC TTCAGCAGTG CCACTTTCTG 
CGGCAGGCTG GCCACTCTGA GAAGGCCATC TCATTGTTCC AGGCCATGGT GGACTTCACC 
TTCTTCAAAC CCGACAGCGT GAAAGATCTG CCTACCAAAG GACAGGTGGA ATTCTTTGAA 
CCCTTTTGGG ACAGTGGAGA GCCCCGGGCT GGGGAGAAGG GAGCCCGAGG CTGGAAGGCG 
TGGATGCACC AGCAGGAACG AGGTGGCTGG GTGGTCATCA ACCCAGATGA GGATGACGAT 
GAACCAGAAG AGGATGACCA GGAAATAAAA GATAAGACTC TGCCCAGGTG GCAGATCTGG 
CTTGCTGCTG AGCGTTCCCG TGACCAGAGG CACTGGCGGC CCTGGCGCCC TGATAAGACC 
AAGAAGCAAA CCGAGGAAGA CTGTGAGGAT CCCGAGAGAC AGGTGTTGTT TGATGATATT 
GGGCAATCTT TGATCAGACT TTCCAGCCAT GATCTTCAGT TCCAGCTGGT GGAGGCCTTC 
CTGCAGTTCT TGGGTGTGCC TTCTGGCTTT ACTCCTCCAG CCTCCTGTCT TTATCTGGCC 
ATGGATGAGA ACAGCATCTT TGATAATGGA CTTTATGATG AAAAGCCCTT GACTTTTTTC 
AACCCTTTGT TTTCTGGGGC TAGCTGTGTT GGCCGCATGG ATAGGTTGGG CTATCCTCGC 
TGGACCAGGG GTCAGAACCG AGAGGGCGAG GAGTTCATCC GCAATGTCTT CCACCTTGTC 
ATGCCTTTAT TTTCAGGCAA AGAGAAGTCC CAGCTCTGCT TCTCCTGGTT ACAGTATGAG 
ATTGCAAAGG TCATTTGGTG CCTGCACACT AAAAACAAGA AGAGATTAAA GTCTCAAGGG 
AAGAACTGCA AAAAACTAGC CAAGAATCTC CTTAAGGAGC CAGAAAACTG CAACAACTTT 
TGCCTGTGGA AGCAGTATGC ACATCTGGAG TGGTTGCTTG GCAACACGGA GGATGCCAGA 
AAAGTTTTTG ACACAGCACT TGGCATGGCA GGAAGCAGAG AACTGAAAGA CTCTGACCTC 
TGTGAGCTCA GTCTGCTCTA TGCTGAGCTG GAGGTGGAGC TGTCGCCAGA AGTGAGAAGG 
GCTGCCACAG CTCGAGCTGT TCACATATTA ACCAAGCTGA CTGAGAGCAG CCCCTATGGG 
CCCTACACTG GACAGGTGTT GGCTGTTCAC ATTTTGAAAG CGCGAAAGGC TTATGAGCAC 
GCACTGCAGG ACTGTTTGGG TGACAGCTGT GTCTCCAATC CAGCTCCCAC CGATTCCTGT 
AGCCGCCTAA TTAGCCTGGC TAAATGCTTC ATGCTCTTCC AGTATTTGAC CATAGGGATT 
GATGCTGCTG TGCAGATATA CGAACAGGTG TTTGCAAAAC TGAACAGTTC TGTTTTCCCA 
GAAGGCTCTG GCGAGGGGGA CAGTGCCAGC TCCCAGAGTT GGACCAGTGT TCTCGAAGCC 
ATCACACTGA TGCACACGAG CCTGCTGAGA TTCCACATGA AAGTGAGTGT TTACCCGCTG 
GCCCCTCTGC GAGAGGCACT CTCACAGGCT TTAAAGTTGT ATCCAGGCAA CCAGGTTCTT 
TGGAGGTCCT ATGTACAGAT TCAGAATAAG TCCCACAGTG CCAGCAAAAC CAGGAGATTT 
TTTGACACAA TCACCAGGTC TGCCAAACCC TTGGAGCCTT GGTTGTTTGC AATTGAAGCT 
GAGAAACTGA GGAAGAGACT GGTGGAAACT GTCCAGAGGT TAGACGGTAG AGAGATCCAC 
GCCACAATTC CTGAGACCGG CTTAATGCAT CGGATCCAAG CCCTGTTTGA AAATGCCATG 
CGCAGCGACA GTGGCAGCCA GTGCCCCTTG CTGTGGAGGA TGTATTTGAA CTTTCTGGTT 
TCCTTAGGAA ATAAAGAAAG AAGCAAAGGT GTATTCTACA AAGCACTTCA GAATTGCCCT 
TGGGCAAAGG TGTTGTACCT GGACGCCGTG GAGTATTTCC CCGATGAGAT GCAGGAGATC 
CTGGACCTGA TGACTGAGAA GGAGCTCCGG GTGCGCCTGC CGCTGGAGGA GCTGGAGCTG 
CTGCTGGAGG ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.
Genes & development33(9-10)536-549(2019 05)
Wang J,Chen J,Wu G,Zhang H,Du X,Chen S,Zhang L,Wang K,Fan J,Gao S,Wu X,Zhang S,Kuai B,Zhao P,Chi B,Wang L,Li G,Wong CCL,Zhou Y,Li J,Yun C,Cheng H

Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing factor.
RNA (New York, N.Y.)25(3)352-363(2019 03)
Jiao AL,Perales R,Umbreit NT,Haswell JR,Piper ME,Adams BD,Pellman D,Kennedy S,Slack FJ

NRDE-2, the human homolog of fission yeast Nrl1, prevents DNA damage accumulation in human cells.
RNA biology15(7)868-876(2018)
Richard P,Ogami K,Chen Y,Feng S,Moresco JJ,Yates JR 3rd,Manley JL

Joint influence of small-effect genetic variants on human longevity.
Aging2(9)612-20(2010 Sep)
Yashin AI,Wu D,Arbeev KG,Ukraintseva SV

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NRDE2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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