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Home » Gene Synthesis & Molecular Biology Services » Preclinical Grade Plasmids New

Overview

GenScript offers preclinical grade plasmids with the most comprehensive and stringent quality specifications and analysis for your preclinical project needs. Ideal for preclinical in vivo animal studies, from mice to non-human primates, in gene therapy, cell therapy, and vaccine research. Available in quantities of 10 mg and up.

Comprehensive Quality Control

Highly homogenous (≥90% supercoiled).

Endotoxin free (≤0.005 EU/μg).

Quantitative bacterial DNA & protein analysis

Animal- Free Production

Plant based culture media

Enzyme-free chromatographic purification

TSE/BSE Certificate

Guaranteed Quantity

From mg to multi-gram level

High density fermentation

Material archiving
Molecular Biology

Quality Comparison

QC Items Analytical Method Specification
Default QC Items Appearance Visual inspection Colorless, clear, free of precipitate or foreign particles
A260/280 Nanodrop UV absorption 1.8-2.0
Sequence Accuracy Sequencing Sequencing verification match the order requirements
pH pH meter 8.0±0.5 (in TE buffer)
Restriction Analysis Enzyme digestion and electrophoresis DNA bands with expected sizes detected by agarose gel electrophoresis
Homogeneity Agarose gel electrophoresis ≥ 90% supercoiled
Endotoxin Quantitative LAL assay ≤ 0.01 EU/μg
E.coli Genomic DNA3, 5 Quantitative PCR ≤ 5%
Residual RNA Agarose gel electrophoresis Non-detectable at 200 ± 50ng
Residual Host Protein HCP ELISA ≤ 1%
Bio-Burden Test Direct incubation No viable microorganisms in /on culture media
Add-On QC Items Advanced Endotoxin Removal Quantitative LAL assay ≤ 0.005 EU/μg
Mycoplasma Contamination Luminescence detection kits Negative
Detection of Kanamycin ELISA ＜1.125 ng/ml

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EMAIL

gene@genscript.com

PHONE

1-877-436-7274

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