Tier 3 High-Quality Plasmid DNA

Tier 3 Plasmid DNA Preparation Workflow

Core Specifications & QC Standards

Custom Add-Ons, Take Your Plasmid Further

Whole Plasmid Verification (NGS & Nanopore)

Gain full-length sequence confidence with deep coverage across your entire construct

Enhanced Three-Step Purification

Gel Filtration, Affinity Chromatography, and Anion Exchange Chromatography for ultra-clean DNA with minimized host gDNA and total protein impurities

Animal-Free & GLP-like Production

Animal-origin-free production and GLP-style workflows with full documentation to support regulatory submissions and ensure compliance confidence

Custom QC Controls

Tailored QC options including sterile filtration, antibiotic residue testing (ampicillin/kanamycin), mycoplasma/phage detection aligned to your specific release criteria

FAQ

• 1. How and why is Tier 3 plasmid optimized for in vivo studies?

  Tier 3 plasmids are produced via high-density fermentation with strict quality standards to support reliable in vivo animal studies. Each batch delivers:

  • ≥90% supercoiled DNA
  • ≤0.005 EU/µg endotoxin
  • ≤5% host genomic DNA
  • ≤1% total protein

  These QC benchmarks and production process ensure low immunogenicity, reduced variability, and high reproducibility in in vivo testing. Tier 3 also supports advanced options add-ons, such as three-step purification (AC, IEX, gel filtration), animal-free or antibiotics-free production, and GLP-like documentation, for added confidence in safety and regulatory compliance.

• 2. What in vivo applications is it suited for?

  Tier 3 plasmids are designed for use in animal studies that require high-quality DNA, such as vaccine research, antibody expression, gene and cell therapy discovery, and preclinical viral vector development (AAV, lentivirus). The high supercoiling and low impurity levels help ensure consistent performance and reliable readouts in these in vivo applications.

• 3. I don’t see the QC or grade I need. Is customized QC testing available?

  Yes. We offer a suite of standard add-on QC options, including whole plasmid sequencing, sterility, mycoplasma, advanced purification, and more (some not shown above). We also support fully customizable QC testing tailored to your specific needs. Please contact us to discuss your requirements.

• 4. What are the ordering options and lead times?

  Tier 3 plasmid preparation service is available starting from 5 mg and up to gram-scale yields, with turnaround times starting at 11 business days depending on the quantity and QC options selected.

Relative Services