GenXceed Cell-free mRNA Template

A Faster, Smarter Path to IVT-Ready DNA

GenXceed streamlines mRNA template synthesis with an RCA-powered, cell-free synthesis approach—bypassing large-scale fermentation, enhancing purity, and delivering high-yield, IVT-ready DNA with unmatched speed and precision.

As fast as 6 Business Days

Gene Synthesis
& Cloning

100% sequence accuracy
mRNA applied vector
Poly(A) Guarantee

Cell-free
Amplification

Proprietary cell-free amplification with no bacterial contaminants

Enzymatic
Linearization

Complete linearization using restriction enzyme of your choice

DNA
Purification

Streamlined purification to remove residual proteins and impurities for IVT-ready quality

Quality
Control

Ensure delivery of sequence verified, Poly(A) intact, fully linearized, IVT-ready DNA

IVT-Ready
DNA

Optimized for seamless mRNA synthesis, no extra steps needed

As fast as 3 Business Days

Data Driven Performance

Unmatched Purity – Optimized for High-Performance IVT

GenXceed delivers ultra-pure, IVT-ready DNA that drives superior mRNA synthesis performance. As shown below, our templates exhibit:

• Exceptional DNA Purity – Verified by AGE and IEC-HPLC analysis, achieving >98% purity, minimizing unwanted contaminants.
• Ultra-Low Endotoxin & Contaminants – Ensuring a clean template for high-efficiency IVT.
• Poly(A) Integrity Verified – Sanger sequencing confirms a complete and stable Poly(A) tail, essential for translation efficiency and mRNA stability.

Fig. 1. DNA Template Purity by AGE

Fig. 2. DNA Template Purity by IEC-HPLC

Fig. 3. Poly(A) Uniformity by FA5200 Analyzer

Fig. 4. Sanger Sequencing for Poly(A) Validation

Superior IVT Performance

GenXceed mRNA templates deliver unmatched IVT performance—exceptional purity, minimal contaminants, and consistently high expression levels. Engineered for efficiency, our RCA-powered DNA templates ensure seamless translation into reliable, high-quality mRNA, driving superior downstream results.

• IVT mRNA Purity by AGE - Gel electrophoresis confirms the high integrity of IVT mRNA, with no visible degradation or unwanted byproducts.
• Ultra-Low Endotoxin & Contaminants – Ensuring a clean template for high-efficiency IVT.
• Poly(A) Integrity Verified – Sanger sequencing confirms a complete and stable Poly(A) tail, essential for translation efficiency and mRNA stability.

Fig. 5. IVT mRNA Purity by AGE

Fig. 6. IVT mRNA quality comparison of mRNA synthesized from GenScript cell-free DNA templates versus traditional plasmid template via Bioanalyzer

Fig. 7. Comparison of mCherry expression from IVT mRNA synthesized using GenScript cell-free DNA templates versus traditional plasmid template

FAQ

• What makes GenXceed Cell-Free mRNA Template different from traditional plasmid-based DNA production?

  GenXceed utilizes Rolling Circle Amplification (RCA), a cell-free, enzymatic amplification process that eliminates the need for large-scale bacterial culture and plasmid extraction. This results in:

  • Higher purity – No bacterial host DNA contamination
  • Minimal endotoxin levels – Avoids endotoxin accumulation from bacterial fermentation
  • Faster turnaround – As fast as 3 business days, compared to 17-22 days for plasmid-based methods
• How does GenXceed ensure high purity and IVT compatibility?

  With our cell-free amplification process, there is no E. coli involved, eliminating the need for extensive purification steps and enabling us to deliver high-quality DNA with faster turnaround.

  We have shown that the final product consistently meets:

  • Endotoxin < 0.05 EU/μg and residual host DNA < 1% — to ensure cleaner IVT reactions
  • No RNase detected or added — protecting RNA integrity throughout the IVT process
  • Total protein < 0.5% — reducing the risk of enzyme inhibition or interference
• Can GenXceed support customized mRNA template designs?

  Absolutely. Our platform offers flexible design options, including:

  • Gene synthesis with your custom sequence of choice, with optional GenSmart™ codon optimization
  • Clone into: GenScript mRNA applied vectors (various poly(A) tail options), or your own custom mRNA vector
  • Final DNA templates meet the same high quality standards, Poly(A) guaranteed

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