Plasmid Linearization Service

For Consistent and High Quality IVT Templates

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Overview

GenScript’s Plasmid Linearization Service provides mRNA researchers with unparalleled expertise, delivering consistent and superior quality results every time. With our rigorous quality control measures, we guarantee linearized plasmids of the highest purity, ensuring efficiency and scalability for a seamless in vitro transcription reaction. Streamline your research process with our comprehensive one-stop solution, spanning from gene synthesis to providing a ready-to-use linearized template.

Benefits

Extensive Quality Controls

Choose from up to 15 QC options

Scalability

µg to g level

Animal Free Production, endotoxin controls for your mRNA downstream applications

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Molecular Biology

Specification

	For Cloning/Transfection Use	For Animal Study Use
Price	Starting from $89	IQR
Quantity	> 30 µg	> 5 mg
	Default Quality Control
Endotoxin Level	<10 EU/mg (based on plasmid prep grade)	<10 EU/mg
Linearization Purification	Anion exchange chromatography (Circular plasmid) Isopropanol precipitation (Linearized plasmid)	AKTA (Circular plasmid) Anion exchange chromatography (Linearization plasmid)
Linearization Confirmation	Agarose gel electrophoresis (single band)	HPLC (≥ 95%)
Residual Host gDNA	Agarose gel electrophoresis (< 15% plasmid band)	qPCR (< 5%)
Residual Host Cell Protein	N/A	Nano orange < 2%
Residual RNA	Agarose gel electrophoresis	Agarose gel electrophoresis

Additional add-on quality controls

	For Cloning/Transfection Use	For Animal Study Use
Additional Linearization Purification	Magnetic bead purification (<100 µg) Anion exchange chromatography（>100 µg）	N/A
Advance Endotoxin Removal	<0.005 EU/µg quantitative TAL (default), LAL (optional)
Mycoplasma Detection	N/A	Luminescence-based Detection Kit
Kanamycin Detection	N/A	ELISA
HPLC (quantitative linearization)	N/A	Additional cost if >98%
Animal Free Process with TSE/BSE Certification	RNase, BsaI/EcoRI/BspQI , TSE/BSE certification
Bio-burden Assay	48 hrs. YPD and LB
Nicked Plasmid Treatment	T4 Ligase Treated Circularization

Case Study

Pure Linearized Plasmid Lead to Successful IVT Reactions

Utilizing pure linearized plasmid is essential for achieving successful IVT reactions that result in elevated mRNA levels. GenScript's Plasmid Linearization ensures meticulous purification steps and rigorous quality controls, providing researchers with the best quality input for optimal mRNA production.

Pure linearized plasmid lead to successful IVT

Linearized plasmid purity%	Linear plasmid%	IVT yield (μg)
50%	50%	16.8
90%	90%	18.4
95%	95%	24.7
98%	98%	23.8
>98%	100%	24.9

Choosing the Best Restriction Enzymes for Optimal Performance

Selecting the appropriate, high-quality restriction enzymes is essential for the best IVT reactions and maximum mRNA output. Elements like the length of the enzyme recognition sequence, the nature of the cleavage product, enzyme stability, and overall quality can significantly influence your mRNA product and its yield. At GenScript, our PhD-level scientists are on hand to guide and advise on your enzyme choices and address any experimental uncertainties.

Figure 1. The circular mRNA template encoding eGFP was linearized utilizing BspQI and XbaI Restriction Enzymes respectively. IVT mRNA was subsequently produced from these linearized plasmids and examined for quality and protein expression. 293T cells were transfected with 0.1 µg of the mRNA using the Lipofectamine 2000 reagent (Invitrogen). GFP expression was observed 48 hours after transfection. The data indicates that the mRNA derived from the BspQI-linearized template exhibited roughly 1.9 times higher GFP expression compared to the XbaI version.