NRDE2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NRDE2
Entrez Gene ID	100138828
Full Name	NRDE-2, necessary for RNA interference, domain containing
Synonyms	C10H14orf102,C14orf102
General protein information	Preferred Names NRDE-2, necessary for RNA interference, domain containing Names protein NRDE2 homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001206507.1	NP_001193436.1	protein NRDE2 homolog
XM_024997208.1	XP_024852976.1	protein NRDE2 homolog isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	NRDE2	XP_001090586.1
Rattus norvegicus (Norway rat)	Nrde2	XP_234465.5
Arabidopsis thaliana (thale cress)	AT3G17740	NP_188401.1
Homo sapiens (human)	NRDE2	NP_060440.2
Gallus gallus (chicken)	C5H14ORF102	XP_421314.3
Arabidopsis thaliana (thale cress)	AT3G17712	NP_001154620.1
Oryza sativa (rice)	Os02g0772500	NP_001048265.1
Mus musculus (house mouse)	Nrde2	NP_898978.2
Xenopus tropicalis (tropical clawed frog)	nrde2	NP_001072867.1
Canis lupus familiaris (dog)	NRDE2	XP_547952.3
Bos taurus (cattle)	NRDE2	NP_001193436.1
Pan troglodytes (chimpanzee)	NRDE2	XP_001141630.1
Danio rerio (zebrafish)	LOC794348	XP_005158613.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NRDE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRDE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa219373	XM_024997208.1 Latest version!	Bos taurus NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa40528	NM_001206507.1 Latest version!	Bos taurus NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NRDE2 ( XM_024997208.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852976.1 Bos taurus NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219373
Clone ID Related Accession (Same CDS sequence)	XM_024997208.1
Accession Version	XM_024997208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	protein NRDE2 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTCT TCCCCGCCTT TGCAGGTGTC GCCGAGGCTC CGGACAGCGG GAGGAAAGAA 
TTAGACTGGC TGAGCAACCC CAGCTTCCAT GTTGGAGCCA TAACGTCCCT GAGCCGACAC 
ACTCAAGAGA CCACTGCAGC CCCTGTTTCT GAAGGGTCAC CACTGACAAG GAGTCCTCTG 
AAATCAGAGC CTTCAGGTGA AAGTGACGCT AACAGAAAGC CCAAAGAAAC GAGCAGAAAA 
AAGAAGAAAG AGAAAAAGAA GAAAAGGAAG CTTCAGCGCC ACGAGAAAAC CAAGAGAAAA 
CACGGGCCGT CGGGTAGCAG TGGGTCTGAG TCAGACACTG ATTCTGCAAA GGACAGCCCT 
TCCAGAAGCC TCAGAGACAG TAAAAGGGAA TCAGAGAAAC CGAATCAAGG AGACGATGCC 
GCCGCGGATG CCGGACGTCA CTGTGTCTGG CTTGAGGACA TCCAGGCTCT GTCGGGGGAA 
ACCTTCCGAA TGGATAAGAA GCCGGATCCT GCCAACTGGG AGTACAAGTC TCTCTACCGA 
GGAGACATTG CCAGATACAA GAGGAAAGGA GACTCCTGCC TCGGCCTCAA CCCGAAGAAG 
CAATGTATAT CTTGGGAGGG GAATTCCACA GAAAAGAAGC GTTCCCACAA GCACATCGAG 
CGCTACTTTA CGAAGAAGAG CGTGGGGTTG ATGAACGTGG ACGGCCTCGC CGTCAGCAGT 
CAGCCCGAAG CTCTGGCGTT GGAGCCGCTC TCCTTTATCC CGGTGAAGGG CGTGGATGAC 
GCGGCTCCCC CTGCTACCAG CTGGCTGAAC CCTCTGGGGA TTTACGACCA AGCCACTACG 
CAGTGGTTAC AGGGACAGGG TCCTTCAGAG CAGGAGTCCA GGCCACCAGA CTCACAGCCC 
GACAGAGAGA GCGCGCTCCT CAAGGCCAGG GTGGAGGAGT TTAACAGGAA GGTCCGCGAG 
AGCCCTCGGG ACACGGAGCT GTGGATGGCC TTCGTTGCTT TCCAGGTGGA GTTCTTTGAG 
CCCTTCTGGG ACAGCGGCGA GCCCCGGGCC GGGGAGAAGG GCGCCCGAGG CTGGCGAGCC 
TGGATGCACC AGCAGGAGTG GGGCGGCTGG GTGGCCGTCG GCGCAGATGA CGACGACGAG 
GAGCCGGAGG AGGAAGACCA GGAGATTAGA GACAAGGCTC TGCCCCGGTG GCAGATCTGG 
CTGGCCGCCG AGCGGTCCCG AGACCACAGG CACTGGAGGC CGTGGCGCCC GGACAAGGCC 
AGGAAGCAGA CGGAGGAAGA CTGCGAGGAC CCCGAGAGAC AGGTGTTGTT TGACGATCTT 
GGGCAGTCTC TGATCCGACT GTCCAGCCCG GATCTCCAGT TTCAGATGAT GGCGGCCTTC 
CTGCAGTTCC TGGGGGTGCC TTCCGGCTTC AGCCCCCCCA CCCCCTGCCT CTATCTGGCC 
ATGGATGAGA ACAGCATCTT CGACAGCGAA CTTTATGATG AAAAGCCTTT GACTTTCCTC 
GACCTCTCCT GCTCCGGCGT CAGCTGTGTA GGCCGCATGG AGCCCCTGGG TGGCCGGCGC 
TGGCCCCGGG GTCCCAGTCG AGAGGGCGAG GAGTTTATCC GCAATGTCTT CCACCTGGCA 
CTGCCTCTGT TTGCAGGCAG GGAGCGGTCT CAGCTCTGCG TCTCCTGGCT ACGGTACGAG 
ATCACCAAGG TCGTTTGGTG TCTGCACACT AAAAACAAGA AGAGATTAAA GTCACAAGGA 
AAGAACTGCA AAAAACTAGC CAAGAATCTC CTCAAGGAGC CGGACAACCG CAACAGCTTT 
TGCCTCTGGC AGCAGTATGC GCATCTGGAG TGGTTGCTGG GCAACACAGA GGATGCCCGG 
AAGGTCTTCG ACGCGGCCCT GGGCACTGCC GGGAGCCGGG GCCTGGAGGA CCCGGAGCTC 
TGTGAGCTCG GCCTGCTCTA CGCCGCGCTG GAGACGGAGC TGTCGCCGCC CGTGGGCGGG 
GCCGCCCCGG CCCGCGCCGT GCACGTGCTG AGCGGGCTGG CGGAGCGCCG CCCCTACGGG 
CCGTGCACGG GACAGGTGTC GGCTGTGCAC GTTTTGAAGG CTCGGAGGGC ATACGAACAC 
GCGCTGCAGG ACTGGCTGGG GGAGCGCCGC GGCCCCGACC CAGGCCCCCC CGACCGCTTC 
AGCCGCCTGG TCAGCCTGGC GAAGTGCTTC ATGCTCTTCC AGTACCTGAC CGTGGGGGTC 
GACGCCGCTG GGCGCGTCTA CGAGCAGGTG TTTGCGGGGC TCAGGGGCTC TGTCTCCTCG 
GAGGCTGCCG GCCTGGAGGG CGCCCTGGAG GCCGTCACCC TGATGCACAC CAGCCTGCTC 
AGGTTCCACG GCAGGGTTGC CGCCCACCCG CTGGCTCCCC TGCGAGAGGC GCTCTCAGAG 
GCTTTACGGT TGTACCCGGG CAACCAGCTT CTCTGGAGGG CGTACGTGCA GATTCAGAGC 
AAGTCGCACA GCGCCAGCAG GACCAGAAGA TTCTTCCACG CCATCACCAG GTCCACCAAA 
CTCCTGGAGC CCTGGCTGTT TGCGATCGAA GCGGAGAGGA TGCGGAAGAG GCTGGTGGAC 
GCCGTGCAGA GGGTGGACGG TAGAGAGGTC CATGCCACCC TTCCCGAGAC CGGCCTGACG 
CACCGGATCA GAGCCCTGTT TGAGAGCGCC GTGCGGAGCG ACCACGGCAG CCAGTGCCCC 
CTGCTGTGGA GGATGTACCT GAACTTCCTG GTTTCCTTAG GAAATAAAGA AAGAAGCAAA 
GGTGTGTTCT ACAAAGCACT GCAGAATTGT CCTTGGGCAA AGGCGCTGTA CATGGATGCC 
GTCGAGTACT TCCCCGACGA GATGCAGGAG GTCGTGGACC TGATGACGGA GAAGGAGCTG 
CGGGTGCGCC TGCCGCTCGA GGAGCTGGAG CTGCTGCTGG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852976.1
CDS	116..3043
Translation	MALFPAFAGVAEAPDSGRKELDWLSNPSFHVGAITSLSRHTQETTAAPVSEGSPLTRSPLKSEPSGESDANRKPKETSRKKKKEKKKKRKLQRHEKTKRKHGPSGSSGSESDTDSAKDSPSRSLRDSKRESEKPNQGDDAAADAGRHCVWLEDIQALSGETFRMDKKPDPANWEYKSLYRGDIARYKRKGDSCLGLNPKKQCISWEGNSTEKKRSHKHIERYFTKKSVGLMNVDGLAVSSQPEALALEPLSFIPVKGVDDAAPPATSWLNPLGIYDQATTQWLQGQGPSEQESRPPDSQPDRESALLKARVEEFNRKVRESPRDTELWMAFVAFQVEFFEPFWDSGEPRAGEKGARGWRAWMHQQEWGGWVAVGADDDDEEPEEEDQEIRDKALPRWQIWLAAERSRDHRHWRPWRPDKARKQTEEDCEDPERQVLFDDLGQSLIRLSSPDLQFQMMAAFLQFLGVPSGFSPPTPCLYLAMDENSIFDSELYDEKPLTFLDLSCSGVSCVGRMEPLGGRRWPRGPSREGEEFIRNVFHLALPLFAGRERSQLCVSWLRYEITKVVWCLHTKNKKRLKSQGKNCKKLAKNLLKEPDNRNSFCLWQQYAHLEWLLGNTEDARKVFDAALGTAGSRGLEDPELCELGLLYAALETELSPPVGGAAPARAVHVLSGLAERRPYGPCTGQVSAVHVLKARRAYEHALQDWLGERRGPDPGPPDRFSRLVSLAKCFMLFQYLTVGVDAAGRVYEQVFAGLRGSVSSEAAGLEGALEAVTLMHTSLLRFHGRVAAHPLAPLREALSEALRLYPGNQLLWRAYVQIQSKSHSASRTRRFFHAITRSTKLLEPWLFAIEAERMRKRLVDAVQRVDGREVHATLPETGLTHRIRALFESAVRSDHGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKALYMDAVEYFPDEMQEVVDLMTEKELRVRLPLEELELLLED

Target ORF information:

RefSeq Version	XM_024997208.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NRDE-2, necessary for RNA interference, domain containing (NRDE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997208.1

ORF Insert Sequence:

ATGGCGCTCT TCCCCGCCTT TGCAGGTGTC GCCGAGGCTC CGGACAGCGG GAGGAAAGAA 
TTAGACTGGC TGAGCAACCC CAGCTTCCAT GTTGGAGCCA TAACGTCCCT GAGCCGACAC 
ACTCAAGAGA CCACTGCAGC CCCTGTTTCT GAAGGGTCAC CACTGACAAG GAGTCCTCTG 
AAATCAGAGC CTTCAGGTGA AAGTGACGCT AACAGAAAGC CCAAAGAAAC GAGCAGAAAA 
AAGAAGAAAG AGAAAAAGAA GAAAAGGAAG CTTCAGCGCC ACGAGAAAAC CAAGAGAAAA 
CACGGGCCGT CGGGTAGCAG TGGGTCTGAG TCAGACACTG ATTCTGCAAA GGACAGCCCT 
TCCAGAAGCC TCAGAGACAG TAAAAGGGAA TCAGAGAAAC CGAATCAAGG AGACGATGCC 
GCCGCGGATG CCGGACGTCA CTGTGTCTGG CTTGAGGACA TCCAGGCTCT GTCGGGGGAA 
ACCTTCCGAA TGGATAAGAA GCCGGATCCT GCCAACTGGG AGTACAAGTC TCTCTACCGA 
GGAGACATTG CCAGATACAA GAGGAAAGGA GACTCCTGCC TCGGCCTCAA CCCGAAGAAG 
CAATGTATAT CTTGGGAGGG GAATTCCACA GAAAAGAAGC GTTCCCACAA GCACATCGAG 
CGCTACTTTA CGAAGAAGAG CGTGGGGTTG ATGAACGTGG ACGGCCTCGC CGTCAGCAGT 
CAGCCCGAAG CTCTGGCGTT GGAGCCGCTC TCCTTTATCC CGGTGAAGGG CGTGGATGAC 
GCGGCTCCCC CTGCTACCAG CTGGCTGAAC CCTCTGGGGA TTTACGACCA AGCCACTACG 
CAGTGGTTAC AGGGACAGGG TCCTTCAGAG CAGGAGTCCA GGCCACCAGA CTCACAGCCC 
GACAGAGAGA GCGCGCTCCT CAAGGCCAGG GTGGAGGAGT TTAACAGGAA GGTCCGCGAG 
AGCCCTCGGG ACACGGAGCT GTGGATGGCC TTCGTTGCTT TCCAGGTGGA GTTCTTTGAG 
CCCTTCTGGG ACAGCGGCGA GCCCCGGGCC GGGGAGAAGG GCGCCCGAGG CTGGCGAGCC 
TGGATGCACC AGCAGGAGTG GGGCGGCTGG GTGGCCGTCG GCGCAGATGA CGACGACGAG 
GAGCCGGAGG AGGAAGACCA GGAGATTAGA GACAAGGCTC TGCCCCGGTG GCAGATCTGG 
CTGGCCGCCG AGCGGTCCCG AGACCACAGG CACTGGAGGC CGTGGCGCCC GGACAAGGCC 
AGGAAGCAGA CGGAGGAAGA CTGCGAGGAC CCCGAGAGAC AGGTGTTGTT TGACGATCTT 
GGGCAGTCTC TGATCCGACT GTCCAGCCCG GATCTCCAGT TTCAGATGAT GGCGGCCTTC 
CTGCAGTTCC TGGGGGTGCC TTCCGGCTTC AGCCCCCCCA CCCCCTGCCT CTATCTGGCC 
ATGGATGAGA ACAGCATCTT CGACAGCGAA CTTTATGATG AAAAGCCTTT GACTTTCCTC 
GACCTCTCCT GCTCCGGCGT CAGCTGTGTA GGCCGCATGG AGCCCCTGGG TGGCCGGCGC 
TGGCCCCGGG GTCCCAGTCG AGAGGGCGAG GAGTTTATCC GCAATGTCTT CCACCTGGCA 
CTGCCTCTGT TTGCAGGCAG GGAGCGGTCT CAGCTCTGCG TCTCCTGGCT ACGGTACGAG 
ATCACCAAGG TCGTTTGGTG TCTGCACACT AAAAACAAGA AGAGATTAAA GTCACAAGGA 
AAGAACTGCA AAAAACTAGC CAAGAATCTC CTCAAGGAGC CGGACAACCG CAACAGCTTT 
TGCCTCTGGC AGCAGTATGC GCATCTGGAG TGGTTGCTGG GCAACACAGA GGATGCCCGG 
AAGGTCTTCG ACGCGGCCCT GGGCACTGCC GGGAGCCGGG GCCTGGAGGA CCCGGAGCTC 
TGTGAGCTCG GCCTGCTCTA CGCCGCGCTG GAGACGGAGC TGTCGCCGCC CGTGGGCGGG 
GCCGCCCCGG CCCGCGCCGT GCACGTGCTG AGCGGGCTGG CGGAGCGCCG CCCCTACGGG 
CCGTGCACGG GACAGGTGTC GGCTGTGCAC GTTTTGAAGG CTCGGAGGGC ATACGAACAC 
GCGCTGCAGG ACTGGCTGGG GGAGCGCCGC GGCCCCGACC CAGGCCCCCC CGACCGCTTC 
AGCCGCCTGG TCAGCCTGGC GAAGTGCTTC ATGCTCTTCC AGTACCTGAC CGTGGGGGTC 
GACGCCGCTG GGCGCGTCTA CGAGCAGGTG TTTGCGGGGC TCAGGGGCTC TGTCTCCTCG 
GAGGCTGCCG GCCTGGAGGG CGCCCTGGAG GCCGTCACCC TGATGCACAC CAGCCTGCTC 
AGGTTCCACG GCAGGGTTGC CGCCCACCCG CTGGCTCCCC TGCGAGAGGC GCTCTCAGAG 
GCTTTACGGT TGTACCCGGG CAACCAGCTT CTCTGGAGGG CGTACGTGCA GATTCAGAGC 
AAGTCGCACA GCGCCAGCAG GACCAGAAGA TTCTTCCACG CCATCACCAG GTCCACCAAA 
CTCCTGGAGC CCTGGCTGTT TGCGATCGAA GCGGAGAGGA TGCGGAAGAG GCTGGTGGAC 
GCCGTGCAGA GGGTGGACGG TAGAGAGGTC CATGCCACCC TTCCCGAGAC CGGCCTGACG 
CACCGGATCA GAGCCCTGTT TGAGAGCGCC GTGCGGAGCG ACCACGGCAG CCAGTGCCCC 
CTGCTGTGGA GGATGTACCT GAACTTCCTG GTTTCCTTAG GAAATAAAGA AAGAAGCAAA 
GGTGTGTTCT ACAAAGCACT GCAGAATTGT CCTTGGGCAA AGGCGCTGTA CATGGATGCC 
GTCGAGTACT TCCCCGACGA GATGCAGGAG GTCGTGGACC TGATGACGGA GAAGGAGCTG 
CGGGTGCGCC TGCCGCTCGA GGAGCTGGAG CTGCTGCTGG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRDE2 ( NM_001206507.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193436.1 Bos taurus NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.

Price & Availability↑

CloneID	OBa40528
Clone ID Related Accession (Same CDS sequence)	NM_001206507.1
Accession Version	NM_001206507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1527436800000
Organism	Bos taurus(cattle)
Product	protein NRDE2 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000010.1. On or before May 4, 2011 this sequence version replaced XM_002691113.1, XM_001788996.2. Sequence Note: This RefSeq record was created from genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on full-length homologous protein alignment with human NP_060440.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTCT TCCCCGCCTT TGCAGGTGTC GCCGAGGCTC CGGACAGCGG GAGGAAAGAA 
TTAGACTGGC TGAGCAACCC CAGCTTCCAT GTTGGAGCCA TAACGTCCCT GAGCCGACAC 
ACTCAAGAGA CCACTGCAGC CCCTGTTTCT GAAGGGTCAC CACTGACAAG GAGTCCTCTG 
AAATCAGAGC CTTCAGGTGA AAGTGACGCT AACAGAAAGC CCAAAGAAAC GAGCAGAAAA 
AAGAAGAAAG AGAAAAAGAA GAAAAGGAAG CTTCAGCGCC ACGAGAAAAC CAAGAGAAAA 
CACGGGCCGT CGGGTAGCAG TGGGTCTGAG TCAGACACTG ATTCTGCAAA GGACAGCCCT 
TCCAGAAGCC TCAGAGACAG TAAAAGGGAA TCAGAGAAAC CGAATCAAGG AGACGATGCC 
GCCGCGGATG CCGGACGTCA CTGTGTCTGG CTTGAGGACA TCCAGGCTCT GTCGGGGGAA 
ACCTTCCGAA TGGATAAGAA GCCGGATCCT GCCAACTGGG AGTACAAGTC TCTCTACCGA 
GGAGACATTG CCAGATACAA GAGGAAAGGA GACTCCTGCC TCGGCCTCAA CCCGAAGAAG 
CAATGTATAT CTTGGGAGGG GAATTCCACA GAAAAGAAGC GTTCCCACAA GCACATCGAG 
CGCTACTTTA CGAAGAAGAG CGTGGGGTTG ATGAACGTGG ACGGCCTCGC CGTCAGCAGT 
CAGCCCGAAG CTCTGGCGTT GGAGCCGCTC TCCTTTATCC CGGTGAAGGG CGTGGATGAC 
GCGGCTCCCC CTGCTACCAG CTGGCTGAAC CCTCTGGGGA TTTACGACCA AGCCACTACG 
CAGTGGTTAC AGGGACAGGG TCCTTCAGAG CAGGAGTCCA GGCCACCAGA CTCACAGCCC 
GACAGAGAGA GCGCGCTCCT CAAGGCCAGG GTGGAGGAGT TTAACAGGAA GGTCCGCGAG 
AGCCCTCGGG ACACGGAGCT GTGGATGGCC TTCGTTGCTT TCCAGGACGA GCTCATGAGA 
AGTCCGGGCC TCTATGCCGT GGAGGAAGGA GAGCCGGAGA CGCGGAAGCG GTCCCTCCGG 
CTGGTCCTGG AGAAGAAGCT GGCCATCCTG GAGCGGGCCA TCGAGAGCAA CCCGAGCAGT 
GTGGACCTGA AGCTCGCCAA GCTGAAGCTC TGCGCCGAGT TCTGGGAGCC CGCCACTCTG 
CTCAAAGAGT GGCAGAAACT GATCTTCTTA CATCCCAACA ACACAGCCCT CTGGCAGAAA 
TACCTGTTGT TCTGCCAGAG CCAGTTCAGC ACCTTCTCCG TATCAAAAAT TCAGAGTCTG 
TACGGGAAGT GCTTGAGCAC CCTGTCCGCC GTGAGGGACG GCAGCATCGT CTCCCACCCC 
GAGCTGCCCG GCACCGAGGA GGCCATGTTT GCCCTCTTCC TCCAGCAGTG CCACTTCCTT 
CGGCAGGCTG GCCACTCCGA GAAGGCCGTG TCTCTGTTCC AGGCCATGGT CGACTTCACG 
TTCTTCAAGC CGGACAGCGT GAAGGGCCTG CCCACCAAAG GCCAGGTGGA GTTCTTTGAG 
CCCTTCTGGG ACAGCGGCGA GCCCCGGGCC GGGGAGAAGG GCGCCCGAGG CTGGCGAGCC 
TGGATGCACC AGCAGGAGTG GGGCGGCTGG GTGGCCGTCG GCGCAGATGA CGACGACGAG 
GAGCCGGAGG AGGAAGACCA GGAGATTAGA GACAAGGCTC TGCCCCGGTG GCAGATCTGG 
CTGGCCGCCG AGCGGTCCCG AGACCACAGG CACTGGAGGC CGTGGCGCCC GGACAAGGCC 
AGGAAGCAGA CGGAGGAAGA CTGCGAGGAC CCCGAGAGAC AGGTGTTGTT TGACGATCTT 
GGGCAGTCTC TGATCCGACT GTCCAGCCCG GATCTCCAGT TTCAGATGAT GGCGGCCTTC 
CTGCAGTTCC TGGGGGTGCC TTCCGGCTTC AGCCCCCCCA CCCCCTGCCT CTATCTGGCC 
ATGGATGAGA ACAGCATCTT CGACAGCGAA CTTTATGATG AAAAGCCTTT GACTTTCCTC 
GACCTCTCCT GCTCCGGCGT CAGCTGTGTA GGCCGCATGG AGCCCCTGGG TGGCCGGCGC 
TGGCCCCGGG GTCCCAGTCG AGAGGGCGAG GAGTTTATCC GCAATGTCTT CCACCTGGCA 
CTGCCTCTGT TTGCAGGCAG GGAGCGGTCT CAGCTCTGCG TCTCCTGGCT ACGGTACGAG 
ATCACCAAGG TCGTTTGGTG TCTGCACACT AAAAACAAGA AGAGATTAAA GTCACAAGGA 
AAGAACTGCA AAAAACTAGC CAAGAATCTC CTCAAGGAGC CGGACAACCG CAACAGCTTT 
TGCCTCTGGC AGCAGTATGC GCATCTGGAG TGGTTGCTGG GCAACACAGA GGATGCCCGG 
AAGGTCTTCG ACGCGGCCCT GGGCACTGCC GGGAGCCGGG GCCTGGAGGA CCCGGAGCTC 
TGTGAGCTCG GCCTGCTCTA CGCCGCGCTG GAGACGGAGC TGTCGCCGCC CGTGGGCGGG 
GCCGCCCCGG CCCGCGCCGT GCACGTGCTG AGCGGGCTGG CGGAGCGCCG CCCCTACGGG 
CCGTGCACGG GACAGGTGTC GGCTGTGCAC GTTTTGAAGG CTCGGAGGGC ATACGAACAC 
GCGCTGCAGG ACTGGCTGGG GGAGCGCCGC GGCCCCGACC CAGGCCCCCC CGACCGCTTC 
AGCCGCCTGG TCAGCCTGGC GAAGTGCTTC ATGCTCTTCC AGTACCTGAC CGTGGGGGTC 
GACGCCGCTG GGCGCGTCTA CGAGCAGGTG TTTGCGGGGC TCAGGGGCTC TGTCTCCTCG 
GAGGCTGCCG GCCTGGAGGG CGCCCTGGAG GCCGTCACCC TGATGCACAC CAGCCTGCTC 
AGGTTCCACG GCAGGGTTGC CGCCCACCCG CTGGCTCCCC TGCGAGAGGC GCTCTCAGAG 
GCTTTACGGT TGTACCCGGG CAACCAGCTT CTCTGGAGGG CGTACGTGCA GATTCAGAGC 
AAGTCGCACA GCGCCAGCAG GACCAGAAGA TTCTTCCACG CCATCACCAG GTCCACCAAA 
CTCCTGGAGC CCTGGCTGTT TGCGATCGAA GCGGAGAGGA TGCGGAAGAG GCTGGTGGAC 
GCCGTGCAGA GGGTGGACGG TAGAGAGGTC CATGCCACCC TTCCCGAGAC CGGCCTGACG 
CACCGGATCA GAGCCCTGTT TGAGAGCGCC GTGCGGAGCG ACCACGGCAG CCAGTGCCCC 
CTGCTGTGGA GGATGTACCT GAACTTCCTG GTTTCCTTAG GAAATAAAGA AAGAAGCAAA 
GGTGTGTTCT ACAAAGCACT GCAGAATTGT CCTTGGGCAA AGGCGCTGTA CATGGATGCC 
GTCGAGTACT TCCCCGACGA GATGCAGGAG GTCGTGGACC TGATGACGGA GAAGGAGCTG 
CGGGTGCGCC TGCCGCTCGA GGAGCTGGAG CTGCTGCTGG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193436.1
CDS	38..3505
Translation	MALFPAFAGVAEAPDSGRKELDWLSNPSFHVGAITSLSRHTQETTAAPVSEGSPLTRSPLKSEPSGESDANRKPKETSRKKKKEKKKKRKLQRHEKTKRKHGPSGSSGSESDTDSAKDSPSRSLRDSKRESEKPNQGDDAAADAGRHCVWLEDIQALSGETFRMDKKPDPANWEYKSLYRGDIARYKRKGDSCLGLNPKKQCISWEGNSTEKKRSHKHIERYFTKKSVGLMNVDGLAVSSQPEALALEPLSFIPVKGVDDAAPPATSWLNPLGIYDQATTQWLQGQGPSEQESRPPDSQPDRESALLKARVEEFNRKVRESPRDTELWMAFVAFQDELMRSPGLYAVEEGEPETRKRSLRLVLEKKLAILERAIESNPSSVDLKLAKLKLCAEFWEPATLLKEWQKLIFLHPNNTALWQKYLLFCQSQFSTFSVSKIQSLYGKCLSTLSAVRDGSIVSHPELPGTEEAMFALFLQQCHFLRQAGHSEKAVSLFQAMVDFTFFKPDSVKGLPTKGQVEFFEPFWDSGEPRAGEKGARGWRAWMHQQEWGGWVAVGADDDDEEPEEEDQEIRDKALPRWQIWLAAERSRDHRHWRPWRPDKARKQTEEDCEDPERQVLFDDLGQSLIRLSSPDLQFQMMAAFLQFLGVPSGFSPPTPCLYLAMDENSIFDSELYDEKPLTFLDLSCSGVSCVGRMEPLGGRRWPRGPSREGEEFIRNVFHLALPLFAGRERSQLCVSWLRYEITKVVWCLHTKNKKRLKSQGKNCKKLAKNLLKEPDNRNSFCLWQQYAHLEWLLGNTEDARKVFDAALGTAGSRGLEDPELCELGLLYAALETELSPPVGGAAPARAVHVLSGLAERRPYGPCTGQVSAVHVLKARRAYEHALQDWLGERRGPDPGPPDRFSRLVSLAKCFMLFQYLTVGVDAAGRVYEQVFAGLRGSVSSEAAGLEGALEAVTLMHTSLLRFHGRVAAHPLAPLREALSEALRLYPGNQLLWRAYVQIQSKSHSASRTRRFFHAITRSTKLLEPWLFAIEAERMRKRLVDAVQRVDGREVHATLPETGLTHRIRALFESAVRSDHGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKALYMDAVEYFPDEMQEVVDLMTEKELRVRLPLEELELLLED

Target ORF information:

RefSeq Version	NM_001206507.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NRDE-2, necessary for RNA interference, domain containing (NRDE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206507.1

ORF Insert Sequence:

ATGGCGCTCT TCCCCGCCTT TGCAGGTGTC GCCGAGGCTC CGGACAGCGG GAGGAAAGAA 
TTAGACTGGC TGAGCAACCC CAGCTTCCAT GTTGGAGCCA TAACGTCCCT GAGCCGACAC 
ACTCAAGAGA CCACTGCAGC CCCTGTTTCT GAAGGGTCAC CACTGACAAG GAGTCCTCTG 
AAATCAGAGC CTTCAGGTGA AAGTGACGCT AACAGAAAGC CCAAAGAAAC GAGCAGAAAA 
AAGAAGAAAG AGAAAAAGAA GAAAAGGAAG CTTCAGCGCC ACGAGAAAAC CAAGAGAAAA 
CACGGGCCGT CGGGTAGCAG TGGGTCTGAG TCAGACACTG ATTCTGCAAA GGACAGCCCT 
TCCAGAAGCC TCAGAGACAG TAAAAGGGAA TCAGAGAAAC CGAATCAAGG AGACGATGCC 
GCCGCGGATG CCGGACGTCA CTGTGTCTGG CTTGAGGACA TCCAGGCTCT GTCGGGGGAA 
ACCTTCCGAA TGGATAAGAA GCCGGATCCT GCCAACTGGG AGTACAAGTC TCTCTACCGA 
GGAGACATTG CCAGATACAA GAGGAAAGGA GACTCCTGCC TCGGCCTCAA CCCGAAGAAG 
CAATGTATAT CTTGGGAGGG GAATTCCACA GAAAAGAAGC GTTCCCACAA GCACATCGAG 
CGCTACTTTA CGAAGAAGAG CGTGGGGTTG ATGAACGTGG ACGGCCTCGC CGTCAGCAGT 
CAGCCCGAAG CTCTGGCGTT GGAGCCGCTC TCCTTTATCC CGGTGAAGGG CGTGGATGAC 
GCGGCTCCCC CTGCTACCAG CTGGCTGAAC CCTCTGGGGA TTTACGACCA AGCCACTACG 
CAGTGGTTAC AGGGACAGGG TCCTTCAGAG CAGGAGTCCA GGCCACCAGA CTCACAGCCC 
GACAGAGAGA GCGCGCTCCT CAAGGCCAGG GTGGAGGAGT TTAACAGGAA GGTCCGCGAG 
AGCCCTCGGG ACACGGAGCT GTGGATGGCC TTCGTTGCTT TCCAGGACGA GCTCATGAGA 
AGTCCGGGCC TCTATGCCGT GGAGGAAGGA GAGCCGGAGA CGCGGAAGCG GTCCCTCCGG 
CTGGTCCTGG AGAAGAAGCT GGCCATCCTG GAGCGGGCCA TCGAGAGCAA CCCGAGCAGT 
GTGGACCTGA AGCTCGCCAA GCTGAAGCTC TGCGCCGAGT TCTGGGAGCC CGCCACTCTG 
CTCAAAGAGT GGCAGAAACT GATCTTCTTA CATCCCAACA ACACAGCCCT CTGGCAGAAA 
TACCTGTTGT TCTGCCAGAG CCAGTTCAGC ACCTTCTCCG TATCAAAAAT TCAGAGTCTG 
TACGGGAAGT GCTTGAGCAC CCTGTCCGCC GTGAGGGACG GCAGCATCGT CTCCCACCCC 
GAGCTGCCCG GCACCGAGGA GGCCATGTTT GCCCTCTTCC TCCAGCAGTG CCACTTCCTT 
CGGCAGGCTG GCCACTCCGA GAAGGCCGTG TCTCTGTTCC AGGCCATGGT CGACTTCACG 
TTCTTCAAGC CGGACAGCGT GAAGGGCCTG CCCACCAAAG GCCAGGTGGA GTTCTTTGAG 
CCCTTCTGGG ACAGCGGCGA GCCCCGGGCC GGGGAGAAGG GCGCCCGAGG CTGGCGAGCC 
TGGATGCACC AGCAGGAGTG GGGCGGCTGG GTGGCCGTCG GCGCAGATGA CGACGACGAG 
GAGCCGGAGG AGGAAGACCA GGAGATTAGA GACAAGGCTC TGCCCCGGTG GCAGATCTGG 
CTGGCCGCCG AGCGGTCCCG AGACCACAGG CACTGGAGGC CGTGGCGCCC GGACAAGGCC 
AGGAAGCAGA CGGAGGAAGA CTGCGAGGAC CCCGAGAGAC AGGTGTTGTT TGACGATCTT 
GGGCAGTCTC TGATCCGACT GTCCAGCCCG GATCTCCAGT TTCAGATGAT GGCGGCCTTC 
CTGCAGTTCC TGGGGGTGCC TTCCGGCTTC AGCCCCCCCA CCCCCTGCCT CTATCTGGCC 
ATGGATGAGA ACAGCATCTT CGACAGCGAA CTTTATGATG AAAAGCCTTT GACTTTCCTC 
GACCTCTCCT GCTCCGGCGT CAGCTGTGTA GGCCGCATGG AGCCCCTGGG TGGCCGGCGC 
TGGCCCCGGG GTCCCAGTCG AGAGGGCGAG GAGTTTATCC GCAATGTCTT CCACCTGGCA 
CTGCCTCTGT TTGCAGGCAG GGAGCGGTCT CAGCTCTGCG TCTCCTGGCT ACGGTACGAG 
ATCACCAAGG TCGTTTGGTG TCTGCACACT AAAAACAAGA AGAGATTAAA GTCACAAGGA 
AAGAACTGCA AAAAACTAGC CAAGAATCTC CTCAAGGAGC CGGACAACCG CAACAGCTTT 
TGCCTCTGGC AGCAGTATGC GCATCTGGAG TGGTTGCTGG GCAACACAGA GGATGCCCGG 
AAGGTCTTCG ACGCGGCCCT GGGCACTGCC GGGAGCCGGG GCCTGGAGGA CCCGGAGCTC 
TGTGAGCTCG GCCTGCTCTA CGCCGCGCTG GAGACGGAGC TGTCGCCGCC CGTGGGCGGG 
GCCGCCCCGG CCCGCGCCGT GCACGTGCTG AGCGGGCTGG CGGAGCGCCG CCCCTACGGG 
CCGTGCACGG GACAGGTGTC GGCTGTGCAC GTTTTGAAGG CTCGGAGGGC ATACGAACAC 
GCGCTGCAGG ACTGGCTGGG GGAGCGCCGC GGCCCCGACC CAGGCCCCCC CGACCGCTTC 
AGCCGCCTGG TCAGCCTGGC GAAGTGCTTC ATGCTCTTCC AGTACCTGAC CGTGGGGGTC 
GACGCCGCTG GGCGCGTCTA CGAGCAGGTG TTTGCGGGGC TCAGGGGCTC TGTCTCCTCG 
GAGGCTGCCG GCCTGGAGGG CGCCCTGGAG GCCGTCACCC TGATGCACAC CAGCCTGCTC 
AGGTTCCACG GCAGGGTTGC CGCCCACCCG CTGGCTCCCC TGCGAGAGGC GCTCTCAGAG 
GCTTTACGGT TGTACCCGGG CAACCAGCTT CTCTGGAGGG CGTACGTGCA GATTCAGAGC 
AAGTCGCACA GCGCCAGCAG GACCAGAAGA TTCTTCCACG CCATCACCAG GTCCACCAAA 
CTCCTGGAGC CCTGGCTGTT TGCGATCGAA GCGGAGAGGA TGCGGAAGAG GCTGGTGGAC 
GCCGTGCAGA GGGTGGACGG TAGAGAGGTC CATGCCACCC TTCCCGAGAC CGGCCTGACG 
CACCGGATCA GAGCCCTGTT TGAGAGCGCC GTGCGGAGCG ACCACGGCAG CCAGTGCCCC 
CTGCTGTGGA GGATGTACCT GAACTTCCTG GTTTCCTTAG GAAATAAAGA AAGAAGCAAA 
GGTGTGTTCT ACAAAGCACT GCAGAATTGT CCTTGGGCAA AGGCGCTGTA CATGGATGCC 
GTCGAGTACT TCCCCGACGA GATGCAGGAG GTCGTGGACC TGATGACGGA GAAGGAGCTG 
CGGGTGCGCC TGCCGCTCGA GGAGCTGGAG CTGCTGCTGG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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NRDE2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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