nrde2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nrde2
Entrez Gene ID	794348
Full Name	NRDE-2, necessary for RNA interference, domain containing
Synonyms	c14orf102
General protein information	Preferred Names NRDE-2, necessary for RNA interference, domain containing Names protein NRDE2 homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005158556.3	XP_005158613.1	protein NRDE2 homolog
XM_005158556.4	XP_005158613.1	protein NRDE2 homolog

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	NRDE2	XP_001090586.1
Rattus norvegicus (Norway rat)	Nrde2	XP_234465.5
Arabidopsis thaliana (thale cress)	AT3G17740	NP_188401.1
Homo sapiens (human)	NRDE2	NP_060440.2
Gallus gallus (chicken)	C5H14ORF102	XP_421314.3
Arabidopsis thaliana (thale cress)	AT3G17712	NP_001154620.1
Oryza sativa (rice)	Os02g0772500	NP_001048265.1
Mus musculus (house mouse)	Nrde2	NP_898978.2
Xenopus tropicalis (tropical clawed frog)	nrde2	NP_001072867.1
Canis lupus familiaris (dog)	NRDE2	XP_547952.3
Bos taurus (cattle)	NRDE2	NP_001193436.1
Pan troglodytes (chimpanzee)	NRDE2	XP_001141630.1
Danio rerio (zebrafish)	LOC794348	XP_005158613.1

Gene Ontology

The following nrde2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nrde2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa22561	XM_005158556.4 Latest version!	Danio rerio NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa22561	XM_005158556.3	Danio rerio NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nrde2 ( XM_005158556.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158613.1 Danio rerio NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.

Price & Availability↑

CloneID	ODa22561
Clone ID Related Accession (Same CDS sequence)	XM_005158556.3 , XM_005158556.4
Accession Version	XM_005158556.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein NRDE2 homolog
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158556.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT TCCCGTCCTT CTCTGGACTG TCAGGCAGCA ACAGCTCTGC AGAAAGTAAA 
GCTCCAGCGG ATCTGGAATG GCTGAGTAAT CAGAGCTTCA GCACTGAAGA TGCTTTGAAG 
ACTCACCAGC GAGCCACAGA CAGAGCCCGA GCAGAGACAG AGGAACCGGC AGGCACAAGT 
GAGGAGAAGC ATAAAGAGAG AGGAGACAAC CATGGAAAAT CGAAGAAAAG AAAGAAAGAA 
AAAAAGAAAA AACACAAAAA GAAGAGGAGC AGAGATAACT CTGAAAGTAG TGGGTTCGAA 
TCAGACACCA TATATCCCAG TGACCTTCTA AAAAAGGAGA ACACTGACAG AGAGGAAGCT 
CAAGTTCGGG TGAGCGAGAC CTTTATGTGG CTGGATGACC TCCAGACCCC CACTGACTCC 
CCCTTCTGCA TCGACAGGAG AGCTGACCGT GCCAACTGGC AATACAAATC CCTTTATAGG 
GGAGACATAG CCAGGTATAA AAGGAAGGGA GGCTCGTGTC TAGGGTTGGA CTTGAGAACG 
CAGGCTGTGA CCTGGACTGA TGGAGGACCA GAGAAGAAGC GAGTGGACAA GAAAGCAGAA 
CGCTATTTCT CTTCTTCCAC CCGTCAGCTG CTTCAAACAA GGGGTCTGCT AGCTTTGCCT 
ATACTCTCTG AAGGCAGCGC CACCAACTCT GATCCCTATA TAAAACTTGC AGTCTGTGCT 
GAAGAACAGG GCTCTTCTAC CCAAACACCT GAATCATGGG TCAATCCGCT GGGCATTTAT 
GATCCTGGAA CCACCTTGTG GCTGGAGGGC AAGGGCCAGC CGGAGGTCAA GGGAGAACAA 
CAGAGCACTG AGGCCCAAGA GAGCAACAGC ACTCTTCTGA GCGCTCGAGT GGAGGAGTTT 
AACAGGAACC TACGGGAGAA TCCTACAGAC ATCAAGCTGT GGTTGGACTT TGTACACTTT 
CAGGATGAGC TGGCCTTCGG TTCTGGGTCT TTCTCTTCTG CCGGTGACTG TGATGCAGAA 
ATGAGGAAGA TGTCTCTACG TGCTGTGCTG GAGAAGAAGC TCTCCATCAT GGACAGAGCA 
GTGGAAAGTA ACCCTTCTGA CGTTGACCTG AAATTAGAAA AACTCCGTCT GTGCAAGGAG 
TTATGGGAGC CTGCCGTCCT GCAGAAAGAG TGGAAGAAAC TAGTGTTCAT TCATCCAAAC 
AGCGCACCTT TATGGAAGAA GTATCTGCTC TTCACACAGA GCCATTTCAG CACCTTCTCA 
GTGTCTAAGG TCAATGGAGT TTTCGGCAAG TGTCTGAGCA CGCTTAGTGC AGTCCAGGAT 
GGCAGCATGG TGTCTCACCC TCCACTTCCA GGCACACAGG AAGACCTCTT GGATATCTTT 
CTGCAGCAGT GCCATTTCCT GCGTCAGGCA GGTCATACAG AGAAAGCTGT TTGCCTTTTT 
CAAGCCCTAC TGGACTTCAC TTTCTTCAAG CCAGACAATG TAAAGAATCT GGCCACCAGA 
CAACAGGTGG ATTTTTTTGA GCCATTTTGG GACAGTGGAG AGCCCAGGAT TGGGGAGCGG 
GGGGCTCGCG GATGGAAGGC TTGGATGCTT CAACAGGAGA AAGGAGGATG GGTGATTCCA 
CCTGAGCCAG ATGATGACGA AGAGGAAGAG CAGGAGGACT CTGAGATCAA AGACAAAACT 
TGGCCTAAGT GGAAGATCTG GTTAGATGTG GAGGCGTCCC GAGAGGCAAA CCAATGGCTG 
CCATGGAGAC CAGATAAAAC CAAGGGCCAA TCAGAAGAGG ACTGCGAGGA CCCAGACAGA 
CAGGTCTTAT TTGATGATAT TGGCCCATCC ATGATTCGTG TTGACAGAGC AGATCTCCAG 
GCGCAGCTAA TTCTGGCATT CCTGCAGTTC CTCGGTCTCC CCGGAGCTCT AACCAAGTTC 
TTTACAGCAT CTTCTTCTAG CTTTCTGCTG GACGATCTCA CCTTCCTTGA AGAGGGCCCT 
GATCCGGAGC GACCACTGAC CTCTTATGAC CTCCCAGTGA CTGGGATCTG TGCTGTGGGT 
CACATGACAT GTCTTAGTGG ATGCAGGAGG CAAGCAGGGC TGTGTAAAGC TGGAGAGGAG 
TTTCTTCACA ATGTTTTGCA TCAAACTCTC CGACTGGTCC CTGCACAGGA TCAAGCTGCT 
ATTACTCTGT GCTGGCTGCA GTACGAGAAA CTCAAGGTGC TGAGGTGTTT ACGAAGTGGA 
AATAAAAGAC AAATGAAGTC TCAGGGGAAG CGCAGTAAAC GTCTTGCAAA AAGGCTGCTA 
AAGGAGCCAG AGAACAGGGG CAGTCTGGCA CTTTGGAGAG AATATGCCCA TCTGGAGTGG 
CTTCTAGGAA ATCTGGAAGA GGCTCGAAAA GTTTTTGACA CTGCTTTGAG TATGGGTGTT 
TCTAGGGGTC TTAATGACCA TGTTCTCTGC AAGCTCTGCT TTCTTTACGC CCAGCTAGAG 
GTAGAACAGG CTTTAAGCAG TGAAACAGTT TCTACATTCT CCAAAGCTGT CTACATTCTG 
ACTAAATTAG CAGAAGGTGC TTCCTACACT CCTTTCTCTG GCCAGATCAA CCCAGTGGTC 
ATATTGAAGG CCAGAAAGGC CTATGAGCAG GCCCTGTTGA GTATTTTACC CGAACAGAAC 
ACAGACAGCA ATGCTGGAGC TTCAAAGAAA CACAATAGGA CGTCCAGTCT AATTGGATGT 
TTTGGACTTT TTCAGTACCT AACAATGGGG ATCGATGCAG CACAAGCTGT GTACAGTCAG 
GCCATCCAAA AACTCATTCC CAGCCATTTC AATTCAGAAG CAGCAGAGGG ATCTCGGAGA 
AGATCCACGA TCTTTGCGGA CTGTGAGGCT GTTGCTGTCC AGCATGTAGC ACTGCTGCGC 
CATCACACTA ACACTAATGT GTTCCCCCTA TCCCGACTGA GACTGGCACT TACAGATGCG 
CTCTCCTTAC TGCCCTCCAG TGCCTCATTA TGGCATCTCT ACCTGCAAAC AGAGAACCAC 
TACCACAGCG CAGGCCGAAC TCGCAGGTTC TTTCACAGCA TGGCAAAGAA AACTGACAGT 
GTTGTGCCGT ATCTCTTCGC CATCACTGCA GAACAACGTC TTAAACAGTT GCTGGACTCT 
GTGCAAAGGT CTCGTCTTCC TGGTGAAGCT CTTCCCACCC TGCCAGTGAA TGGTCTTAGC 
AATCGTATCC GTTGTCTGTT TGAAAGCGCT ACAGGAACAG AGCATGGAGC CCACTGTCCT 
CTGCTGTGGA GAATGTATCT GAACTTCATG GTGTGTGATG GGAATGCAGA CAGAGGCAGT 
GGAATCTTTT ACAAGGCTTT ACAGGAAGTG CCCTGGGTAA AGGGCTTGTA CATGGATGCA 
GTGCAGCTGT TTCCTGAACG TGTGCAGGAG TTTTTAGACC TCATGACTGA GAAGGAGTTG 
CGTCTGAGAA TGCCCATGGA GGAGGTGGAC ATCCTGCTGG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158613.1
CDS	81..3548
Translation	MALFPSFSGLSGSNSSAESKAPADLEWLSNQSFSTEDALKTHQRATDRARAETEEPAGTSEEKHKERGDNHGKSKKRKKEKKKKHKKKRSRDNSESSGFESDTIYPSDLLKKENTDREEAQVRVSETFMWLDDLQTPTDSPFCIDRRADRANWQYKSLYRGDIARYKRKGGSCLGLDLRTQAVTWTDGGPEKKRVDKKAERYFSSSTRQLLQTRGLLALPILSEGSATNSDPYIKLAVCAEEQGSSTQTPESWVNPLGIYDPGTTLWLEGKGQPEVKGEQQSTEAQESNSTLLSARVEEFNRNLRENPTDIKLWLDFVHFQDELAFGSGSFSSAGDCDAEMRKMSLRAVLEKKLSIMDRAVESNPSDVDLKLEKLRLCKELWEPAVLQKEWKKLVFIHPNSAPLWKKYLLFTQSHFSTFSVSKVNGVFGKCLSTLSAVQDGSMVSHPPLPGTQEDLLDIFLQQCHFLRQAGHTEKAVCLFQALLDFTFFKPDNVKNLATRQQVDFFEPFWDSGEPRIGERGARGWKAWMLQQEKGGWVIPPEPDDDEEEEQEDSEIKDKTWPKWKIWLDVEASREANQWLPWRPDKTKGQSEEDCEDPDRQVLFDDIGPSMIRVDRADLQAQLILAFLQFLGLPGALTKFFTASSSSFLLDDLTFLEEGPDPERPLTSYDLPVTGICAVGHMTCLSGCRRQAGLCKAGEEFLHNVLHQTLRLVPAQDQAAITLCWLQYEKLKVLRCLRSGNKRQMKSQGKRSKRLAKRLLKEPENRGSLALWREYAHLEWLLGNLEEARKVFDTALSMGVSRGLNDHVLCKLCFLYAQLEVEQALSSETVSTFSKAVYILTKLAEGASYTPFSGQINPVVILKARKAYEQALLSILPEQNTDSNAGASKKHNRTSSLIGCFGLFQYLTMGIDAAQAVYSQAIQKLIPSHFNSEAAEGSRRRSTIFADCEAVAVQHVALLRHHTNTNVFPLSRLRLALTDALSLLPSSASLWHLYLQTENHYHSAGRTRRFFHSMAKKTDSVVPYLFAITAEQRLKQLLDSVQRSRLPGEALPTLPVNGLSNRIRCLFESATGTEHGAHCPLLWRMYLNFMVCDGNADRGSGIFYKALQEVPWVKGLYMDAVQLFPERVQEFLDLMTEKELRLRMPMEEVDILLEE

Target ORF information:

RefSeq Version	XM_005158556.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158556.3

ORF Insert Sequence:

ATGGCTCTGT TCCCGTCCTT CTCTGGACTG TCAGGCAGCA ACAGCTCTGC AGAAAGTAAA 
GCTCCAGCGG ATCTGGAATG GCTGAGTAAT CAGAGCTTCA GCACTGAAGA TGCTTTGAAG 
ACTCACCAGC GAGCCACAGA CAGAGCCCGA GCAGAGACAG AGGAACCGGC AGGCACAAGT 
GAGGAGAAGC ATAAAGAGAG AGGAGACAAC CATGGAAAAT CGAAGAAAAG AAAGAAAGAA 
AAAAAGAAAA AACACAAAAA GAAGAGGAGC AGAGATAACT CTGAAAGTAG TGGGTTCGAA 
TCAGACACCA TATATCCCAG TGACCTTCTA AAAAAGGAGA ACACTGACAG AGAGGAAGCT 
CAAGTTCGGG TGAGCGAGAC CTTTATGTGG CTGGATGACC TCCAGACCCC CACTGACTCC 
CCCTTCTGCA TCGACAGGAG AGCTGACCGT GCCAACTGGC AATACAAATC CCTTTATAGG 
GGAGACATAG CCAGGTATAA AAGGAAGGGA GGCTCGTGTC TAGGGTTGGA CTTGAGAACG 
CAGGCTGTGA CCTGGACTGA TGGAGGACCA GAGAAGAAGC GAGTGGACAA GAAAGCAGAA 
CGCTATTTCT CTTCTTCCAC CCGTCAGCTG CTTCAAACAA GGGGTCTGCT AGCTTTGCCT 
ATACTCTCTG AAGGCAGCGC CACCAACTCT GATCCCTATA TAAAACTTGC AGTCTGTGCT 
GAAGAACAGG GCTCTTCTAC CCAAACACCT GAATCATGGG TCAATCCGCT GGGCATTTAT 
GATCCTGGAA CCACCTTGTG GCTGGAGGGC AAGGGCCAGC CGGAGGTCAA GGGAGAACAA 
CAGAGCACTG AGGCCCAAGA GAGCAACAGC ACTCTTCTGA GCGCTCGAGT GGAGGAGTTT 
AACAGGAACC TACGGGAGAA TCCTACAGAC ATCAAGCTGT GGTTGGACTT TGTACACTTT 
CAGGATGAGC TGGCCTTCGG TTCTGGGTCT TTCTCTTCTG CCGGTGACTG TGATGCAGAA 
ATGAGGAAGA TGTCTCTACG TGCTGTGCTG GAGAAGAAGC TCTCCATCAT GGACAGAGCA 
GTGGAAAGTA ACCCTTCTGA CGTTGACCTG AAATTAGAAA AACTCCGTCT GTGCAAGGAG 
TTATGGGAGC CTGCCGTCCT GCAGAAAGAG TGGAAGAAAC TAGTGTTCAT TCATCCAAAC 
AGCGCACCTT TATGGAAGAA GTATCTGCTC TTCACACAGA GCCATTTCAG CACCTTCTCA 
GTGTCTAAGG TCAATGGAGT TTTCGGCAAG TGTCTGAGCA CGCTTAGTGC AGTCCAGGAT 
GGCAGCATGG TGTCTCACCC TCCACTTCCA GGCACACAGG AAGACCTCTT GGATATCTTT 
CTGCAGCAGT GCCATTTCCT GCGTCAGGCA GGTCATACAG AGAAAGCTGT TTGCCTTTTT 
CAAGCCCTAC TGGACTTCAC TTTCTTCAAG CCAGACAATG TAAAGAATCT GGCCACCAGA 
CAACAGGTGG ATTTTTTTGA GCCATTTTGG GACAGTGGAG AGCCCAGGAT TGGGGAGCGG 
GGGGCTCGCG GATGGAAGGC TTGGATGCTT CAACAGGAGA AAGGAGGATG GGTGATTCCA 
CCTGAGCCAG ATGATGACGA AGAGGAAGAG CAGGAGGACT CTGAGATCAA AGACAAAACT 
TGGCCTAAGT GGAAGATCTG GTTAGATGTG GAGGCGTCCC GAGAGGCAAA CCAATGGCTG 
CCATGGAGAC CAGATAAAAC CAAGGGCCAA TCAGAAGAGG ACTGCGAGGA CCCAGACAGA 
CAGGTCTTAT TTGATGATAT TGGCCCATCC ATGATTCGTG TTGACAGAGC AGATCTCCAG 
GCGCAGCTAA TTCTGGCATT CCTGCAGTTC CTCGGTCTCC CCGGAGCTCT AACCAAGTTC 
TTTACAGCAT CTTCTTCTAG CTTTCTGCTG GACGATCTCA CCTTCCTTGA AGAGGGCCCT 
GATCCGGAGC GACCACTGAC CTCTTATGAC CTCCCAGTGA CTGGGATCTG TGCTGTGGGT 
CACATGACAT GTCTTAGTGG ATGCAGGAGG CAAGCAGGGC TGTGTAAAGC TGGAGAGGAG 
TTTCTTCACA ATGTTTTGCA TCAAACTCTC CGACTGGTCC CTGCACAGGA TCAAGCTGCT 
ATTACTCTGT GCTGGCTGCA GTACGAGAAA CTCAAGGTGC TGAGGTGTTT ACGAAGTGGA 
AATAAAAGAC AAATGAAGTC TCAGGGGAAG CGCAGTAAAC GTCTTGCAAA AAGGCTGCTA 
AAGGAGCCAG AGAACAGGGG CAGTCTGGCA CTTTGGAGAG AATATGCCCA TCTGGAGTGG 
CTTCTAGGAA ATCTGGAAGA GGCTCGAAAA GTTTTTGACA CTGCTTTGAG TATGGGTGTT 
TCTAGGGGTC TTAATGACCA TGTTCTCTGC AAGCTCTGCT TTCTTTACGC CCAGCTAGAG 
GTAGAACAGG CTTTAAGCAG TGAAACAGTT TCTACATTCT CCAAAGCTGT CTACATTCTG 
ACTAAATTAG CAGAAGGTGC TTCCTACACT CCTTTCTCTG GCCAGATCAA CCCAGTGGTC 
ATATTGAAGG CCAGAAAGGC CTATGAGCAG GCCCTGTTGA GTATTTTACC CGAACAGAAC 
ACAGACAGCA ATGCTGGAGC TTCAAAGAAA CACAATAGGA CGTCCAGTCT AATTGGATGT 
TTTGGACTTT TTCAGTACCT AACAATGGGG ATCGATGCAG CACAAGCTGT GTACAGTCAG 
GCCATCCAAA AACTCATTCC CAGCCATTTC AATTCAGAAG CAGCAGAGGG ATCTCGGAGA 
AGATCCACGA TCTTTGCGGA CTGTGAGGCT GTTGCTGTCC AGCATGTAGC ACTGCTGCGC 
CATCACACTA ACACTAATGT GTTCCCCCTA TCCCGACTGA GACTGGCACT TACAGATGCG 
CTCTCCTTAC TGCCCTCCAG TGCCTCATTA TGGCATCTCT ACCTGCAAAC AGAGAACCAC 
TACCACAGCG CAGGCCGAAC TCGCAGGTTC TTTCACAGCA TGGCAAAGAA AACTGACAGT 
GTTGTGCCGT ATCTCTTCGC CATCACTGCA GAACAACGTC TTAAACAGTT GCTGGACTCT 
GTGCAAAGGT CTCGTCTTCC TGGTGAAGCT CTTCCCACCC TGCCAGTGAA TGGTCTTAGC 
AATCGTATCC GTTGTCTGTT TGAAAGCGCT ACAGGAACAG AGCATGGAGC CCACTGTCCT 
CTGCTGTGGA GAATGTATCT GAACTTCATG GTGTGTGATG GGAATGCAGA CAGAGGCAGT 
GGAATCTTTT ACAAGGCTTT ACAGGAAGTG CCCTGGGTAA AGGGCTTGTA CATGGATGCA 
GTGCAGCTGT TTCCTGAACG TGTGCAGGAG TTTTTAGACC TCATGACTGA GAAGGAGTTG 
CGTCTGAGAA TGCCCATGGA GGAGGTGGAC ATCCTGCTGG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrde2 ( XM_005158556.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158613.1 Danio rerio NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.

Price & Availability↑

CloneID	ODa22561
Clone ID Related Accession (Same CDS sequence)	XM_005158556.3 , XM_005158556.4
Accession Version	XM_005158556.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein NRDE2 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158556.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT TCCCGTCCTT CTCTGGACTG TCAGGCAGCA ACAGCTCTGC AGAAAGTAAA 
GCTCCAGCGG ATCTGGAATG GCTGAGTAAT CAGAGCTTCA GCACTGAAGA TGCTTTGAAG 
ACTCACCAGC GAGCCACAGA CAGAGCCCGA GCAGAGACAG AGGAACCGGC AGGCACAAGT 
GAGGAGAAGC ATAAAGAGAG AGGAGACAAC CATGGAAAAT CGAAGAAAAG AAAGAAAGAA 
AAAAAGAAAA AACACAAAAA GAAGAGGAGC AGAGATAACT CTGAAAGTAG TGGGTTCGAA 
TCAGACACCA TATATCCCAG TGACCTTCTA AAAAAGGAGA ACACTGACAG AGAGGAAGCT 
CAAGTTCGGG TGAGCGAGAC CTTTATGTGG CTGGATGACC TCCAGACCCC CACTGACTCC 
CCCTTCTGCA TCGACAGGAG AGCTGACCGT GCCAACTGGC AATACAAATC CCTTTATAGG 
GGAGACATAG CCAGGTATAA AAGGAAGGGA GGCTCGTGTC TAGGGTTGGA CTTGAGAACG 
CAGGCTGTGA CCTGGACTGA TGGAGGACCA GAGAAGAAGC GAGTGGACAA GAAAGCAGAA 
CGCTATTTCT CTTCTTCCAC CCGTCAGCTG CTTCAAACAA GGGGTCTGCT AGCTTTGCCT 
ATACTCTCTG AAGGCAGCGC CACCAACTCT GATCCCTATA TAAAACTTGC AGTCTGTGCT 
GAAGAACAGG GCTCTTCTAC CCAAACACCT GAATCATGGG TCAATCCGCT GGGCATTTAT 
GATCCTGGAA CCACCTTGTG GCTGGAGGGC AAGGGCCAGC CGGAGGTCAA GGGAGAACAA 
CAGAGCACTG AGGCCCAAGA GAGCAACAGC ACTCTTCTGA GCGCTCGAGT GGAGGAGTTT 
AACAGGAACC TACGGGAGAA TCCTACAGAC ATCAAGCTGT GGTTGGACTT TGTACACTTT 
CAGGATGAGC TGGCCTTCGG TTCTGGGTCT TTCTCTTCTG CCGGTGACTG TGATGCAGAA 
ATGAGGAAGA TGTCTCTACG TGCTGTGCTG GAGAAGAAGC TCTCCATCAT GGACAGAGCA 
GTGGAAAGTA ACCCTTCTGA CGTTGACCTG AAATTAGAAA AACTCCGTCT GTGCAAGGAG 
TTATGGGAGC CTGCCGTCCT GCAGAAAGAG TGGAAGAAAC TAGTGTTCAT TCATCCAAAC 
AGCGCACCTT TATGGAAGAA GTATCTGCTC TTCACACAGA GCCATTTCAG CACCTTCTCA 
GTGTCTAAGG TCAATGGAGT TTTCGGCAAG TGTCTGAGCA CGCTTAGTGC AGTCCAGGAT 
GGCAGCATGG TGTCTCACCC TCCACTTCCA GGCACACAGG AAGACCTCTT GGATATCTTT 
CTGCAGCAGT GCCATTTCCT GCGTCAGGCA GGTCATACAG AGAAAGCTGT TTGCCTTTTT 
CAAGCCCTAC TGGACTTCAC TTTCTTCAAG CCAGACAATG TAAAGAATCT GGCCACCAGA 
CAACAGGTGG ATTTTTTTGA GCCATTTTGG GACAGTGGAG AGCCCAGGAT TGGGGAGCGG 
GGGGCTCGCG GATGGAAGGC TTGGATGCTT CAACAGGAGA AAGGAGGATG GGTGATTCCA 
CCTGAGCCAG ATGATGACGA AGAGGAAGAG CAGGAGGACT CTGAGATCAA AGACAAAACT 
TGGCCTAAGT GGAAGATCTG GTTAGATGTG GAGGCGTCCC GAGAGGCAAA CCAATGGCTG 
CCATGGAGAC CAGATAAAAC CAAGGGCCAA TCAGAAGAGG ACTGCGAGGA CCCAGACAGA 
CAGGTCTTAT TTGATGATAT TGGCCCATCC ATGATTCGTG TTGACAGAGC AGATCTCCAG 
GCGCAGCTAA TTCTGGCATT CCTGCAGTTC CTCGGTCTCC CCGGAGCTCT AACCAAGTTC 
TTTACAGCAT CTTCTTCTAG CTTTCTGCTG GACGATCTCA CCTTCCTTGA AGAGGGCCCT 
GATCCGGAGC GACCACTGAC CTCTTATGAC CTCCCAGTGA CTGGGATCTG TGCTGTGGGT 
CACATGACAT GTCTTAGTGG ATGCAGGAGG CAAGCAGGGC TGTGTAAAGC TGGAGAGGAG 
TTTCTTCACA ATGTTTTGCA TCAAACTCTC CGACTGGTCC CTGCACAGGA TCAAGCTGCT 
ATTACTCTGT GCTGGCTGCA GTACGAGAAA CTCAAGGTGC TGAGGTGTTT ACGAAGTGGA 
AATAAAAGAC AAATGAAGTC TCAGGGGAAG CGCAGTAAAC GTCTTGCAAA AAGGCTGCTA 
AAGGAGCCAG AGAACAGGGG CAGTCTGGCA CTTTGGAGAG AATATGCCCA TCTGGAGTGG 
CTTCTAGGAA ATCTGGAAGA GGCTCGAAAA GTTTTTGACA CTGCTTTGAG TATGGGTGTT 
TCTAGGGGTC TTAATGACCA TGTTCTCTGC AAGCTCTGCT TTCTTTACGC CCAGCTAGAG 
GTAGAACAGG CTTTAAGCAG TGAAACAGTT TCTACATTCT CCAAAGCTGT CTACATTCTG 
ACTAAATTAG CAGAAGGTGC TTCCTACACT CCTTTCTCTG GCCAGATCAA CCCAGTGGTC 
ATATTGAAGG CCAGAAAGGC CTATGAGCAG GCCCTGTTGA GTATTTTACC CGAACAGAAC 
ACAGACAGCA ATGCTGGAGC TTCAAAGAAA CACAATAGGA CGTCCAGTCT AATTGGATGT 
TTTGGACTTT TTCAGTACCT AACAATGGGG ATCGATGCAG CACAAGCTGT GTACAGTCAG 
GCCATCCAAA AACTCATTCC CAGCCATTTC AATTCAGAAG CAGCAGAGGG ATCTCGGAGA 
AGATCCACGA TCTTTGCGGA CTGTGAGGCT GTTGCTGTCC AGCATGTAGC ACTGCTGCGC 
CATCACACTA ACACTAATGT GTTCCCCCTA TCCCGACTGA GACTGGCACT TACAGATGCG 
CTCTCCTTAC TGCCCTCCAG TGCCTCATTA TGGCATCTCT ACCTGCAAAC AGAGAACCAC 
TACCACAGCG CAGGCCGAAC TCGCAGGTTC TTTCACAGCA TGGCAAAGAA AACTGACAGT 
GTTGTGCCGT ATCTCTTCGC CATCACTGCA GAACAACGTC TTAAACAGTT GCTGGACTCT 
GTGCAAAGGT CTCGTCTTCC TGGTGAAGCT CTTCCCACCC TGCCAGTGAA TGGTCTTAGC 
AATCGTATCC GTTGTCTGTT TGAAAGCGCT ACAGGAACAG AGCATGGAGC CCACTGTCCT 
CTGCTGTGGA GAATGTATCT GAACTTCATG GTGTGTGATG GGAATGCAGA CAGAGGCAGT 
GGAATCTTTT ACAAGGCTTT ACAGGAAGTG CCCTGGGTAA AGGGCTTGTA CATGGATGCA 
GTGCAGCTGT TTCCTGAACG TGTGCAGGAG TTTTTAGACC TCATGACTGA GAAGGAGTTG 
CGTCTGAGAA TGCCCATGGA GGAGGTGGAC ATCCTGCTGG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158613.1
CDS	64..3531
Translation	MALFPSFSGLSGSNSSAESKAPADLEWLSNQSFSTEDALKTHQRATDRARAETEEPAGTSEEKHKERGDNHGKSKKRKKEKKKKHKKKRSRDNSESSGFESDTIYPSDLLKKENTDREEAQVRVSETFMWLDDLQTPTDSPFCIDRRADRANWQYKSLYRGDIARYKRKGGSCLGLDLRTQAVTWTDGGPEKKRVDKKAERYFSSSTRQLLQTRGLLALPILSEGSATNSDPYIKLAVCAEEQGSSTQTPESWVNPLGIYDPGTTLWLEGKGQPEVKGEQQSTEAQESNSTLLSARVEEFNRNLRENPTDIKLWLDFVHFQDELAFGSGSFSSAGDCDAEMRKMSLRAVLEKKLSIMDRAVESNPSDVDLKLEKLRLCKELWEPAVLQKEWKKLVFIHPNSAPLWKKYLLFTQSHFSTFSVSKVNGVFGKCLSTLSAVQDGSMVSHPPLPGTQEDLLDIFLQQCHFLRQAGHTEKAVCLFQALLDFTFFKPDNVKNLATRQQVDFFEPFWDSGEPRIGERGARGWKAWMLQQEKGGWVIPPEPDDDEEEEQEDSEIKDKTWPKWKIWLDVEASREANQWLPWRPDKTKGQSEEDCEDPDRQVLFDDIGPSMIRVDRADLQAQLILAFLQFLGLPGALTKFFTASSSSFLLDDLTFLEEGPDPERPLTSYDLPVTGICAVGHMTCLSGCRRQAGLCKAGEEFLHNVLHQTLRLVPAQDQAAITLCWLQYEKLKVLRCLRSGNKRQMKSQGKRSKRLAKRLLKEPENRGSLALWREYAHLEWLLGNLEEARKVFDTALSMGVSRGLNDHVLCKLCFLYAQLEVEQALSSETVSTFSKAVYILTKLAEGASYTPFSGQINPVVILKARKAYEQALLSILPEQNTDSNAGASKKHNRTSSLIGCFGLFQYLTMGIDAAQAVYSQAIQKLIPSHFNSEAAEGSRRRSTIFADCEAVAVQHVALLRHHTNTNVFPLSRLRLALTDALSLLPSSASLWHLYLQTENHYHSAGRTRRFFHSMAKKTDSVVPYLFAITAEQRLKQLLDSVQRSRLPGEALPTLPVNGLSNRIRCLFESATGTEHGAHCPLLWRMYLNFMVCDGNADRGSGIFYKALQEVPWVKGLYMDAVQLFPERVQEFLDLMTEKELRLRMPMEEVDILLEE

Target ORF information:

RefSeq Version	XM_005158556.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NRDE-2, necessary for RNA interference, domain containing (nrde2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158556.4

ORF Insert Sequence:

ATGGCTCTGT TCCCGTCCTT CTCTGGACTG TCAGGCAGCA ACAGCTCTGC AGAAAGTAAA 
GCTCCAGCGG ATCTGGAATG GCTGAGTAAT CAGAGCTTCA GCACTGAAGA TGCTTTGAAG 
ACTCACCAGC GAGCCACAGA CAGAGCCCGA GCAGAGACAG AGGAACCGGC AGGCACAAGT 
GAGGAGAAGC ATAAAGAGAG AGGAGACAAC CATGGAAAAT CGAAGAAAAG AAAGAAAGAA 
AAAAAGAAAA AACACAAAAA GAAGAGGAGC AGAGATAACT CTGAAAGTAG TGGGTTCGAA 
TCAGACACCA TATATCCCAG TGACCTTCTA AAAAAGGAGA ACACTGACAG AGAGGAAGCT 
CAAGTTCGGG TGAGCGAGAC CTTTATGTGG CTGGATGACC TCCAGACCCC CACTGACTCC 
CCCTTCTGCA TCGACAGGAG AGCTGACCGT GCCAACTGGC AATACAAATC CCTTTATAGG 
GGAGACATAG CCAGGTATAA AAGGAAGGGA GGCTCGTGTC TAGGGTTGGA CTTGAGAACG 
CAGGCTGTGA CCTGGACTGA TGGAGGACCA GAGAAGAAGC GAGTGGACAA GAAAGCAGAA 
CGCTATTTCT CTTCTTCCAC CCGTCAGCTG CTTCAAACAA GGGGTCTGCT AGCTTTGCCT 
ATACTCTCTG AAGGCAGCGC CACCAACTCT GATCCCTATA TAAAACTTGC AGTCTGTGCT 
GAAGAACAGG GCTCTTCTAC CCAAACACCT GAATCATGGG TCAATCCGCT GGGCATTTAT 
GATCCTGGAA CCACCTTGTG GCTGGAGGGC AAGGGCCAGC CGGAGGTCAA GGGAGAACAA 
CAGAGCACTG AGGCCCAAGA GAGCAACAGC ACTCTTCTGA GCGCTCGAGT GGAGGAGTTT 
AACAGGAACC TACGGGAGAA TCCTACAGAC ATCAAGCTGT GGTTGGACTT TGTACACTTT 
CAGGATGAGC TGGCCTTCGG TTCTGGGTCT TTCTCTTCTG CCGGTGACTG TGATGCAGAA 
ATGAGGAAGA TGTCTCTACG TGCTGTGCTG GAGAAGAAGC TCTCCATCAT GGACAGAGCA 
GTGGAAAGTA ACCCTTCTGA CGTTGACCTG AAATTAGAAA AACTCCGTCT GTGCAAGGAG 
TTATGGGAGC CTGCCGTCCT GCAGAAAGAG TGGAAGAAAC TAGTGTTCAT TCATCCAAAC 
AGCGCACCTT TATGGAAGAA GTATCTGCTC TTCACACAGA GCCATTTCAG CACCTTCTCA 
GTGTCTAAGG TCAATGGAGT TTTCGGCAAG TGTCTGAGCA CGCTTAGTGC AGTCCAGGAT 
GGCAGCATGG TGTCTCACCC TCCACTTCCA GGCACACAGG AAGACCTCTT GGATATCTTT 
CTGCAGCAGT GCCATTTCCT GCGTCAGGCA GGTCATACAG AGAAAGCTGT TTGCCTTTTT 
CAAGCCCTAC TGGACTTCAC TTTCTTCAAG CCAGACAATG TAAAGAATCT GGCCACCAGA 
CAACAGGTGG ATTTTTTTGA GCCATTTTGG GACAGTGGAG AGCCCAGGAT TGGGGAGCGG 
GGGGCTCGCG GATGGAAGGC TTGGATGCTT CAACAGGAGA AAGGAGGATG GGTGATTCCA 
CCTGAGCCAG ATGATGACGA AGAGGAAGAG CAGGAGGACT CTGAGATCAA AGACAAAACT 
TGGCCTAAGT GGAAGATCTG GTTAGATGTG GAGGCGTCCC GAGAGGCAAA CCAATGGCTG 
CCATGGAGAC CAGATAAAAC CAAGGGCCAA TCAGAAGAGG ACTGCGAGGA CCCAGACAGA 
CAGGTCTTAT TTGATGATAT TGGCCCATCC ATGATTCGTG TTGACAGAGC AGATCTCCAG 
GCGCAGCTAA TTCTGGCATT CCTGCAGTTC CTCGGTCTCC CCGGAGCTCT AACCAAGTTC 
TTTACAGCAT CTTCTTCTAG CTTTCTGCTG GACGATCTCA CCTTCCTTGA AGAGGGCCCT 
GATCCGGAGC GACCACTGAC CTCTTATGAC CTCCCAGTGA CTGGGATCTG TGCTGTGGGT 
CACATGACAT GTCTTAGTGG ATGCAGGAGG CAAGCAGGGC TGTGTAAAGC TGGAGAGGAG 
TTTCTTCACA ATGTTTTGCA TCAAACTCTC CGACTGGTCC CTGCACAGGA TCAAGCTGCT 
ATTACTCTGT GCTGGCTGCA GTACGAGAAA CTCAAGGTGC TGAGGTGTTT ACGAAGTGGA 
AATAAAAGAC AAATGAAGTC TCAGGGGAAG CGCAGTAAAC GTCTTGCAAA AAGGCTGCTA 
AAGGAGCCAG AGAACAGGGG CAGTCTGGCA CTTTGGAGAG AATATGCCCA TCTGGAGTGG 
CTTCTAGGAA ATCTGGAAGA GGCTCGAAAA GTTTTTGACA CTGCTTTGAG TATGGGTGTT 
TCTAGGGGTC TTAATGACCA TGTTCTCTGC AAGCTCTGCT TTCTTTACGC CCAGCTAGAG 
GTAGAACAGG CTTTAAGCAG TGAAACAGTT TCTACATTCT CCAAAGCTGT CTACATTCTG 
ACTAAATTAG CAGAAGGTGC TTCCTACACT CCTTTCTCTG GCCAGATCAA CCCAGTGGTC 
ATATTGAAGG CCAGAAAGGC CTATGAGCAG GCCCTGTTGA GTATTTTACC CGAACAGAAC 
ACAGACAGCA ATGCTGGAGC TTCAAAGAAA CACAATAGGA CGTCCAGTCT AATTGGATGT 
TTTGGACTTT TTCAGTACCT AACAATGGGG ATCGATGCAG CACAAGCTGT GTACAGTCAG 
GCCATCCAAA AACTCATTCC CAGCCATTTC AATTCAGAAG CAGCAGAGGG ATCTCGGAGA 
AGATCCACGA TCTTTGCGGA CTGTGAGGCT GTTGCTGTCC AGCATGTAGC ACTGCTGCGC 
CATCACACTA ACACTAATGT GTTCCCCCTA TCCCGACTGA GACTGGCACT TACAGATGCG 
CTCTCCTTAC TGCCCTCCAG TGCCTCATTA TGGCATCTCT ACCTGCAAAC AGAGAACCAC 
TACCACAGCG CAGGCCGAAC TCGCAGGTTC TTTCACAGCA TGGCAAAGAA AACTGACAGT 
GTTGTGCCGT ATCTCTTCGC CATCACTGCA GAACAACGTC TTAAACAGTT GCTGGACTCT 
GTGCAAAGGT CTCGTCTTCC TGGTGAAGCT CTTCCCACCC TGCCAGTGAA TGGTCTTAGC 
AATCGTATCC GTTGTCTGTT TGAAAGCGCT ACAGGAACAG AGCATGGAGC CCACTGTCCT 
CTGCTGTGGA GAATGTATCT GAACTTCATG GTGTGTGATG GGAATGCAGA CAGAGGCAGT 
GGAATCTTTT ACAAGGCTTT ACAGGAAGTG CCCTGGGTAA AGGGCTTGTA CATGGATGCA 
GTGCAGCTGT TTCCTGAACG TGTGCAGGAG TTTTTAGACC TCATGACTGA GAAGGAGTTG 
CGTCTGAGAA TGCCCATGGA GGAGGTGGAC ATCCTGCTGG AGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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nrde2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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