NOS1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS1
Entrez Gene ID	60658
Full Name	nitric oxide synthase 1 (neuronal)
Synonyms	nNOS,nos
General protein information	Preferred Names nitric oxide synthase 1 (neuronal) Names nitric oxide synthase, brain
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131660.1	NP_571735.1	nitric oxide synthase, brain
XM_005165053.4	XP_005165110.1	nitric oxide synthase, brain isoform X1
XM_005165053.3	XP_005165110.1	nitric oxide synthase, brain isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NOS1	XP_003314004.2
Rattus norvegicus (Norway rat)	Nos1	NP_434686.1
Gallus gallus (chicken)	NOS1	XP_004934537.1
Xenopus tropicalis (tropical clawed frog)	nos1	XP_004910558.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008255	XP_317213.1
Macaca mulatta (Rhesus monkey)	NOS1	XP_001083352.2
Mus musculus (house mouse)	Nos1	NP_032738.1
Homo sapiens (human)	NOS1	NP_000611.1
Bos taurus (cattle)	NOS1	XP_002694631.3
Canis lupus familiaris (dog)	NOS1	NP_001182074.1
Danio rerio (zebrafish)	nos1	NP_571735.1
Drosophila melanogaster (fruit fly)	Nos	NP_523541.2

Gene Ontology
Bibliography

Related articles in PubMed

Nitric oxide interacts with monoamine oxidase to modulate aggression and anxiety-like behaviour.
Carreño Gutiérrez H, O'Leary A, Freudenberg F, Fedele G, Wilkinson R, Markham E, van Eeden F, Reif A, Norton WHJ
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology3030-43(2020 Jan)

Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene.
Nikaido M, Izumi S, Ohnuki H, Takigawa Y, Yamasu K, Hatta K
Gene expression patterns : GEP2812-21(2018 06)

The dual role of cGMP in oocyte maturation of zebrafish.
Li J, Zhou W, Wang Y, Niu C
Biochemical and biophysical research communications499(4)998-1003(2018 05)

Histone acetyltransferase 7 (KAT7)-dependent intragenic histone acetylation regulates endothelial cell gene regulation.
Yan MS, Turgeon PJ, Man HJ, Dubinsky MK, Ho JJD, El-Rass S, Wang YD, Wen XY, Marsden PA
The Journal of biological chemistry293(12)4381-4402(2018 03)

Mycobacterium marinum Infection in Zebrafish and Microglia Imitates the Early Stage of Tuberculous Meningitis.
Chen Z, Shao XY, Wang C, Hua MH, Wang CN, Wang X, Wang QJ, Yao JY, Fan YH, Qin YW
Journal of molecular neuroscience : MN64(2)321-330(2018 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01326	NM_131660.1 Latest version!	Danio rerio nitric oxide synthase 1 (neuronal) (nos1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa39410	XM_005165053.4 Latest version!	Danio rerio nitric oxide synthase 1 (neuronal) (nos1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
ODa39410	XM_005165053.3	Danio rerio nitric oxide synthase 1 (neuronal) (nos1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS1 ( NM_131660.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571735.1 Danio rerio nitric oxide synthase 1 (neuronal) (nos1), mRNA.

Price & Availability↑

CloneID	ODa01326
Clone ID Related Accession (Same CDS sequence)	NM_131660.1
Accession Version	NM_131660.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541174400000
Organism	Danio rerio(zebrafish)
Product	nitric oxide synthase, brain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY211528.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY211528.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA3505370 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGAAT CAGAGCCCAG CGTGTGTCAA CTACAGCCCA ACATCATCTC AGTTCGCCTC 
TTTAAAAGAA AGATCGGTGG ACTGGGCTTT CTGGTCAAGC AGCGGGTGTG CAAACCACCT 
GTCATCGTCT CGGACCTCAT CCGGGGAGGA GCGGCGGAGG AATGCGGGCT TGTCCAAGTG 
GGCGACATCG TCCTTGCTGT AAACAACAAG CCGTTGGTGG ACATGAGTTA TGAACGTGCT 
CTTGAAACCC TGAAGAACGT GTCACCTGAA AGCCACGCTG TCCTGATCCT TCGTGGACCA 
GAGGGTTTCA CCACTCACCT GGAGACCACG CTGAGCAGTG ATGGAAAAAC CAAGACGATT 
CGGGTCACCA GGCCTGTCAA CCCGGCCCAA AGCAGGCCAT GCGAACGATG CTCGCCCCTC 
ACGCCCAACA TGACCAAAGA GCTCAGGGCA ATAGAAAACC TATCCTCCTC CTCCACTCCC 
AGGAAAGAGT CGCGGGATGA CAAACCCCTG ATTCCCCTGC TGTCACAGGA CTCTCTTCTC 
AGAGAAGGAG GCCACGCGGG ACTTCTCCTC AATGGGGTGG AGGATAACAA TGACCTGCTG 
AAAGAGATCG AGCCTGTGCT GAAGCTCATC AAGAACAGCA AAAAAGAGAT CAACGGAGAG 
GGCCAGAGGG TCATCGATAG GAAGGATGCA GAGGTGCAGG TGGAGAGAGA CCTTGGTGTG 
GGAAATGGAC AGAACGCCCA GCTGGCATCG GATAACGACA GAGTGTTTGA TGACCTCTGG 
AGAAAGGACA ACATGCCCAC GGTGCTTAAC AACCCTTACT CAGAAAGTGA CAAGCCACAG 
TCTTATGAAA GAGTTTCTCC TACCAAGGCT GTACAGAACG GAAGTCCCTC CAAATGTCCC 
AGATTCATGA AAGTGAAGAA TTGGGAAACG GGAGCCTTAT ACAATGATAC TTTACACCAC 
AGCTCCAGTA AGGTGCCGAT CTGCACTGAG CACGTTTGCA TAGGGTCTAT TATGGTGCCA 
AACCAGCACG CTCACGAACC AGATGAAGAC AGAAGAAAAG AGGAGCTGCT ACCACTTGCA 
ACTGATTTCA TCGACCAGTA CTACACATCA ATCAAAAGAT GTTGCTCAAA GGCCCATGTT 
GACCGACTAG AAGAAGTCAA AAAAGAAATC GAAACTTCTG GAACATATCA GTTAAAGGAC 
ACAGAGCTAA TCTATGGGGC CAAGCACGCA TGGAGAAATG CAGCTCGCTG TGTGGGACGA 
ATCCAGTGGT CCAAATTACA GGTGTTTGAC GCCCGCGACT GCACAACCGC TCATGGAATG 
TTTAATTACA TCTGCAACCA CATCAAATAC GCCACCAACA AAGGAAACCT CAGATCAGCT 
ATTACCATCT TCCCCCAGAA GACAGATGGG AAGCATGACT TCCGGGTGTG GAACAGCCAG 
CTGATCCGCT ACGCAGGCTA CAAGCAGCCC GATGGATCAA TTCTAGGAGA TCCAGCTTCT 
GTAGAGCTTA CAGAGATCTG TATGCAGCAA GGATGGAAAG CTCCAAAAGG CCGGTTCGAT 
GTTTTGCCCC TTCTTCTCCA AGCCAATGGG AATGATCCAG AGCTTTTTGA GATCCCTGAT 
GATCTTGTAC TTGAGGTCCC CATCATACAT CCAAAGTTTG AATGGTTTAA GGAGCTGAAT 
CTAAAGTGGT ACGGCCTTCC TGCTGTGTCA AATATGCTTT TAGAGATCGG CGGTCTAGAG 
TTCACCGCAT GTCCCTTCAG CGGCTGGTAC ATGGGGACAG AAATTGGTGT GAGAGACTTC 
TGCGACAGCT CTCGATACAA CATACTTGAG GAAGTAGCTA CAATGATGGG TCTGGATACG 
AGGAAGACTT CGTCCCTGTG GAAGGACCAA GCACTGGTGG AGATCAACAT CGCAGTCCTT 
TATAGCTACC AGATGTCAAA GGTTACCATA GTAGACCATC ACTCGGCCAC TGAGTCCTTC 
ATGAAGCACA TGGAGAATGA GTACCGAGTG AGAGGAGGCT GTCCGGGAGA CTGGGTTTGG 
ATAGTGCCAC CCATGTCCGG TAGCATCACA CCTGTATTCC ACCAGGAAAT GCTCAACTAT 
CGCCTCACAC CATCTTTTGA GTACCAGCCT GACCCATGGA ATACTCACGT ATGGAAAGGA 
GTGAATGGGA CGCCAACAAA GAAAAGAGCG ATTGGATTCA AGAAACTTGC CAAGGCTGTA 
AAGTTCTCCG CCACACTCAT GGGACAAGCC ATGGCCAAGA GAGTCAAAGC CACCATCTTA 
TTCGCCACAG AAACGGGGAA ATCGCAGGAC TATGCAAAAA CGCTCTGTGA GATTTTCAAG 
CACGCATTTG ATGCCAAGGT GATGTCTATG GATGAATATG ACACAGTGGA TCTGGAGCAC 
GAGACACTTG TGATAGTCGT GACAAGCACA TTCGGCAACG GTGACCCTCC TGAAAATGGA 
GAGAAATTTG GTGCGGCGCT GATGGAGATG AGACATCCCA CCACCAGCGT AGAGGACAGA 
AAGACGTACA AGGTGCGGTT TAACAGCGTT TCTTCGTATT CTGACACCCG CAAATCCTCC 
AGCGATGAGC CTGAATCAAA AGCCAACTTC GAGAGCACTG GACCGCTTGC TAATGTCAGA 
TTCTCCGTGT TTGGCCTTGG CTCTCGGGCA TACCCACACT TCTGTGCATT TGCCCACGCA 
GTGGACACGC TGTTCGAGGA GCTTGGCAGG GAACGAATCC TGCGCATGGG GGAAGGAGAC 
GAACTCTGCG GCCAGGAGGA ATCCTTCAGA ACGTGGGCTA AGAAAGTTTT TAAGGCTGCA 
TGCGACGTTT TTTGTGTCGG TGATGATGTT AACATCGAGA AAGCAAACGA TTCCCTGATT 
AGTAACGATC GCAGCTGGAA GAAAAGCAAG TTCCGGATGA CATACACGGC TGAAGCACCG 
ACACTAACGC AAGCGCTTTA CAGCATCCAT AAGAAGAAAG TTTACGGAGC CAAAGTTGTG 
CAGAGACAAA ACCTACAAAG TGCCAGATCA AACCGATTGA CGATATTTGT GCGTCTTGAT 
GCAAACAACC ACGAGAGCCT AAAATACCTG CCTGGAGACC ATTTAGGCGT TTTCCCTGGC 
AACAATGAAG ATCTTGTTAC CACTCTTATC GAGAAACTGG AGGATGCGCC GCCTGTCAAT 
CAAATAGTGA AAGTGGAGTT CTTGGAGGAA AGAAACACAG CTCTAGGAGT GATAAGCAAC 
TGGACAGATG AGAGTCGTAT TCCACCGTGC ACCATCTATC AGGCCTTCAA ATACTTTCTG 
GACATAACCA CCCCTCCTAG TCCTGTACTG TTGCAGCAGT TTGCTCCTCT AGCCACTAAC 
GAGAAACAGA GAAAGAGACT AGAGGTGCTC AGCAAGGGTT TGCAGGAGTA CGAGGAATGG 
AAGTGGTACA ATAATCCTAC CATGGTGGAG GTGCTGGAGG AGTTTCCATC CCTCCAGATC 
CCCTCCACCC TACTCTTGAC GCAGCTGCCT CTCCTACAGC CGCGCTACTA CTCCATTAGC 
TCCTCTCCAG ACCTCTATCC TGGAGAGATC CACCTCACTG TGGCCGTGGT GTCCTATCGA 
CCCAGAGATG GAGAAGGGCC TATTCATCAT GGAGTTTGTT CCTCGTGGCT CAACAGCTTG 
GATGAAGGCG ATACAGTGCC GTGCTTTGTC AGAGGGGCTC CATCCTTCCG TATGCCAAAA 
GACAGCCAGG TTCCTTGCAT TCTGATTGGT CCTGGCACTG GAATCGCTCC ATTCAGAGCC 
TTCTGGCAAC AGAGACAGTA CGATGTCGAA AATAAAGGAA TCAAGGCTTG CCCCATGATC 
TTGGTGTTTG GTTGTCGGCA GTCCCAGATT GACCACATTT ACAAGGAACA GACCATCCAA 
GCCAAGAACA AGGAAGTGTT TAAAGAGCTT TACACGGCAT ACTCTAGAGA GCCCGGAAGA 
CCAAAGAAAT ATGTCCAGGA TGTGCTCCGC GAGCAGCTAT CTGAAACTGT GTACAAATGT 
CTAAGAGAAG AAGGAGGACA CATCTATGTC TGTGGGGACG TCACCATGGC CGGAGACGTC 
CTGAAAACTG TCCAGCAGAT CTTTAAACTG CACGGCAACA TGTCTCTGGA AGATGCGGGT 
TTCTATATCA GCAAACTACG GGATGAGAAT CGATACCATG AGGACATTTT TGGTGTAACC 
CTGCGCACTT GCGAGGTCAC CAACCGTCTG AGGTCAGAAT CCATTGCGTA CATCGAGGAG 
AGCAAGAAAG ATATAGATGA GGTGTTTTGC TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571735.1
CDS	13..4308
Translation	MQESEPSVCQLQPNIISVRLFKRKIGGLGFLVKQRVCKPPVIVSDLIRGGAAEECGLVQVGDIVLAVNNKPLVDMSYERALETLKNVSPESHAVLILRGPEGFTTHLETTLSSDGKTKTIRVTRPVNPAQSRPCERCSPLTPNMTKELRAIENLSSSSTPRKESRDDKPLIPLLSQDSLLREGGHAGLLLNGVEDNNDLLKEIEPVLKLIKNSKKEINGEGQRVIDRKDAEVQVERDLGVGNGQNAQLASDNDRVFDDLWRKDNMPTVLNNPYSESDKPQSYERVSPTKAVQNGSPSKCPRFMKVKNWETGALYNDTLHHSSSKVPICTEHVCIGSIMVPNQHAHEPDEDRRKEELLPLATDFIDQYYTSIKRCCSKAHVDRLEEVKKEIETSGTYQLKDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQKTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPASVELTEICMQQGWKAPKGRFDVLPLLLQANGNDPELFEIPDDLVLEVPIIHPKFEWFKELNLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDFCDSSRYNILEEVATMMGLDTRKTSSLWKDQALVEINIAVLYSYQMSKVTIVDHHSATESFMKHMENEYRVRGGCPGDWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGVNGTPTKKRAIGFKKLAKAVKFSATLMGQAMAKRVKATILFATETGKSQDYAKTLCEIFKHAFDAKVMSMDEYDTVDLEHETLVIVVTSTFGNGDPPENGEKFGAALMEMRHPTTSVEDRKTYKVRFNSVSSYSDTRKSSSDEPESKANFESTGPLANVRFSVFGLGSRAYPHFCAFAHAVDTLFEELGRERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANDSLISNDRSWKKSKFRMTYTAEAPTLTQALYSIHKKKVYGAKVVQRQNLQSARSNRLTIFVRLDANNHESLKYLPGDHLGVFPGNNEDLVTTLIEKLEDAPPVNQIVKVEFLEERNTALGVISNWTDESRIPPCTIYQAFKYFLDITTPPSPVLLQQFAPLATNEKQRKRLEVLSKGLQEYEEWKWYNNPTMVEVLEEFPSLQIPSTLLLTQLPLLQPRYYSISSSPDLYPGEIHLTVAVVSYRPRDGEGPIHHGVCSSWLNSLDEGDTVPCFVRGAPSFRMPKDSQVPCILIGPGTGIAPFRAFWQQRQYDVENKGIKACPMILVFGCRQSQIDHIYKEQTIQAKNKEVFKELYTAYSREPGRPKKYVQDVLREQLSETVYKCLREEGGHIYVCGDVTMAGDVLKTVQQIFKLHGNMSLEDAGFYISKLRDENRYHEDIFGVTLRTCEVTNRLRSESIAYIEESKKDIDEVFCS

Target ORF information:

RefSeq Version	NM_131660.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nitric oxide synthase 1 (neuronal) (nos1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131660.1

ORF Insert Sequence:

ATGCAGGAAT CAGAGCCCAG CGTGTGTCAA CTACAGCCCA ACATCATCTC AGTTCGCCTC 
TTTAAAAGAA AGATCGGTGG ACTGGGCTTT CTGGTCAAGC AGCGGGTGTG CAAACCACCT 
GTCATCGTCT CGGACCTCAT CCGGGGAGGA GCGGCGGAGG AATGCGGGCT TGTCCAAGTG 
GGCGACATCG TCCTTGCTGT AAACAACAAG CCGTTGGTGG ACATGAGTTA TGAACGTGCT 
CTTGAAACCC TGAAGAACGT GTCACCTGAA AGCCACGCTG TCCTGATCCT TCGTGGACCA 
GAGGGTTTCA CCACTCACCT GGAGACCACG CTGAGCAGTG ATGGAAAAAC CAAGACGATT 
CGGGTCACCA GGCCTGTCAA CCCGGCCCAA AGCAGGCCAT GCGAACGATG CTCGCCCCTC 
ACGCCCAACA TGACCAAAGA GCTCAGGGCA ATAGAAAACC TATCCTCCTC CTCCACTCCC 
AGGAAAGAGT CGCGGGATGA CAAACCCCTG ATTCCCCTGC TGTCACAGGA CTCTCTTCTC 
AGAGAAGGAG GCCACGCGGG ACTTCTCCTC AATGGGGTGG AGGATAACAA TGACCTGCTG 
AAAGAGATCG AGCCTGTGCT GAAGCTCATC AAGAACAGCA AAAAAGAGAT CAACGGAGAG 
GGCCAGAGGG TCATCGATAG GAAGGATGCA GAGGTGCAGG TGGAGAGAGA CCTTGGTGTG 
GGAAATGGAC AGAACGCCCA GCTGGCATCG GATAACGACA GAGTGTTTGA TGACCTCTGG 
AGAAAGGACA ACATGCCCAC GGTGCTTAAC AACCCTTACT CAGAAAGTGA CAAGCCACAG 
TCTTATGAAA GAGTTTCTCC TACCAAGGCT GTACAGAACG GAAGTCCCTC CAAATGTCCC 
AGATTCATGA AAGTGAAGAA TTGGGAAACG GGAGCCTTAT ACAATGATAC TTTACACCAC 
AGCTCCAGTA AGGTGCCGAT CTGCACTGAG CACGTTTGCA TAGGGTCTAT TATGGTGCCA 
AACCAGCACG CTCACGAACC AGATGAAGAC AGAAGAAAAG AGGAGCTGCT ACCACTTGCA 
ACTGATTTCA TCGACCAGTA CTACACATCA ATCAAAAGAT GTTGCTCAAA GGCCCATGTT 
GACCGACTAG AAGAAGTCAA AAAAGAAATC GAAACTTCTG GAACATATCA GTTAAAGGAC 
ACAGAGCTAA TCTATGGGGC CAAGCACGCA TGGAGAAATG CAGCTCGCTG TGTGGGACGA 
ATCCAGTGGT CCAAATTACA GGTGTTTGAC GCCCGCGACT GCACAACCGC TCATGGAATG 
TTTAATTACA TCTGCAACCA CATCAAATAC GCCACCAACA AAGGAAACCT CAGATCAGCT 
ATTACCATCT TCCCCCAGAA GACAGATGGG AAGCATGACT TCCGGGTGTG GAACAGCCAG 
CTGATCCGCT ACGCAGGCTA CAAGCAGCCC GATGGATCAA TTCTAGGAGA TCCAGCTTCT 
GTAGAGCTTA CAGAGATCTG TATGCAGCAA GGATGGAAAG CTCCAAAAGG CCGGTTCGAT 
GTTTTGCCCC TTCTTCTCCA AGCCAATGGG AATGATCCAG AGCTTTTTGA GATCCCTGAT 
GATCTTGTAC TTGAGGTCCC CATCATACAT CCAAAGTTTG AATGGTTTAA GGAGCTGAAT 
CTAAAGTGGT ACGGCCTTCC TGCTGTGTCA AATATGCTTT TAGAGATCGG CGGTCTAGAG 
TTCACCGCAT GTCCCTTCAG CGGCTGGTAC ATGGGGACAG AAATTGGTGT GAGAGACTTC 
TGCGACAGCT CTCGATACAA CATACTTGAG GAAGTAGCTA CAATGATGGG TCTGGATACG 
AGGAAGACTT CGTCCCTGTG GAAGGACCAA GCACTGGTGG AGATCAACAT CGCAGTCCTT 
TATAGCTACC AGATGTCAAA GGTTACCATA GTAGACCATC ACTCGGCCAC TGAGTCCTTC 
ATGAAGCACA TGGAGAATGA GTACCGAGTG AGAGGAGGCT GTCCGGGAGA CTGGGTTTGG 
ATAGTGCCAC CCATGTCCGG TAGCATCACA CCTGTATTCC ACCAGGAAAT GCTCAACTAT 
CGCCTCACAC CATCTTTTGA GTACCAGCCT GACCCATGGA ATACTCACGT ATGGAAAGGA 
GTGAATGGGA CGCCAACAAA GAAAAGAGCG ATTGGATTCA AGAAACTTGC CAAGGCTGTA 
AAGTTCTCCG CCACACTCAT GGGACAAGCC ATGGCCAAGA GAGTCAAAGC CACCATCTTA 
TTCGCCACAG AAACGGGGAA ATCGCAGGAC TATGCAAAAA CGCTCTGTGA GATTTTCAAG 
CACGCATTTG ATGCCAAGGT GATGTCTATG GATGAATATG ACACAGTGGA TCTGGAGCAC 
GAGACACTTG TGATAGTCGT GACAAGCACA TTCGGCAACG GTGACCCTCC TGAAAATGGA 
GAGAAATTTG GTGCGGCGCT GATGGAGATG AGACATCCCA CCACCAGCGT AGAGGACAGA 
AAGACGTACA AGGTGCGGTT TAACAGCGTT TCTTCGTATT CTGACACCCG CAAATCCTCC 
AGCGATGAGC CTGAATCAAA AGCCAACTTC GAGAGCACTG GACCGCTTGC TAATGTCAGA 
TTCTCCGTGT TTGGCCTTGG CTCTCGGGCA TACCCACACT TCTGTGCATT TGCCCACGCA 
GTGGACACGC TGTTCGAGGA GCTTGGCAGG GAACGAATCC TGCGCATGGG GGAAGGAGAC 
GAACTCTGCG GCCAGGAGGA ATCCTTCAGA ACGTGGGCTA AGAAAGTTTT TAAGGCTGCA 
TGCGACGTTT TTTGTGTCGG TGATGATGTT AACATCGAGA AAGCAAACGA TTCCCTGATT 
AGTAACGATC GCAGCTGGAA GAAAAGCAAG TTCCGGATGA CATACACGGC TGAAGCACCG 
ACACTAACGC AAGCGCTTTA CAGCATCCAT AAGAAGAAAG TTTACGGAGC CAAAGTTGTG 
CAGAGACAAA ACCTACAAAG TGCCAGATCA AACCGATTGA CGATATTTGT GCGTCTTGAT 
GCAAACAACC ACGAGAGCCT AAAATACCTG CCTGGAGACC ATTTAGGCGT TTTCCCTGGC 
AACAATGAAG ATCTTGTTAC CACTCTTATC GAGAAACTGG AGGATGCGCC GCCTGTCAAT 
CAAATAGTGA AAGTGGAGTT CTTGGAGGAA AGAAACACAG CTCTAGGAGT GATAAGCAAC 
TGGACAGATG AGAGTCGTAT TCCACCGTGC ACCATCTATC AGGCCTTCAA ATACTTTCTG 
GACATAACCA CCCCTCCTAG TCCTGTACTG TTGCAGCAGT TTGCTCCTCT AGCCACTAAC 
GAGAAACAGA GAAAGAGACT AGAGGTGCTC AGCAAGGGTT TGCAGGAGTA CGAGGAATGG 
AAGTGGTACA ATAATCCTAC CATGGTGGAG GTGCTGGAGG AGTTTCCATC CCTCCAGATC 
CCCTCCACCC TACTCTTGAC GCAGCTGCCT CTCCTACAGC CGCGCTACTA CTCCATTAGC 
TCCTCTCCAG ACCTCTATCC TGGAGAGATC CACCTCACTG TGGCCGTGGT GTCCTATCGA 
CCCAGAGATG GAGAAGGGCC TATTCATCAT GGAGTTTGTT CCTCGTGGCT CAACAGCTTG 
GATGAAGGCG ATACAGTGCC GTGCTTTGTC AGAGGGGCTC CATCCTTCCG TATGCCAAAA 
GACAGCCAGG TTCCTTGCAT TCTGATTGGT CCTGGCACTG GAATCGCTCC ATTCAGAGCC 
TTCTGGCAAC AGAGACAGTA CGATGTCGAA AATAAAGGAA TCAAGGCTTG CCCCATGATC 
TTGGTGTTTG GTTGTCGGCA GTCCCAGATT GACCACATTT ACAAGGAACA GACCATCCAA 
GCCAAGAACA AGGAAGTGTT TAAAGAGCTT TACACGGCAT ACTCTAGAGA GCCCGGAAGA 
CCAAAGAAAT ATGTCCAGGA TGTGCTCCGC GAGCAGCTAT CTGAAACTGT GTACAAATGT 
CTAAGAGAAG AAGGAGGACA CATCTATGTC TGTGGGGACG TCACCATGGC CGGAGACGTC 
CTGAAAACTG TCCAGCAGAT CTTTAAACTG CACGGCAACA TGTCTCTGGA AGATGCGGGT 
TTCTATATCA GCAAACTACG GGATGAGAAT CGATACCATG AGGACATTTT TGGTGTAACC 
CTGCGCACTT GCGAGGTCAC CAACCGTCTG AGGTCAGAAT CCATTGCGTA CATCGAGGAG 
AGCAAGAAAG ATATAGATGA GGTGTTTTGC TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_005165053.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165110.1 Danio rerio nitric oxide synthase 1 (neuronal) (nos1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39410
Clone ID Related Accession (Same CDS sequence)	XM_005165053.4 , XM_005165053.3
Accession Version	XM_005165053.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165053.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAT CAGAGCCCAG CGTGTGTCAA CTACAGCCCA ACATCATCTC AGTTCGCCTC 
TTTAAAAGAA AGATCGGTGG ACTGGGCTTT CTGGTCAAGC AGCGGGTGTG CAAACCACCT 
GTCATCGTCT CGGACCTCAT CCGGGGAGGA GCGGCGGAGG AATGCGGGCT TGTCCAAGTG 
GGCGACATCG TCCTTGCTGT AAACAACAAG CCGTTGGTGG ACATGAGTTA TGAACGTGCT 
CTTGAAACCC TGAAGAACGT GTCACCCGAA AGCCACGCTG TCCTGATCCT TCGTGGACCA 
GAGGGTTTCA CCACTCACCT GGAGACCACG CTGAGCAGTG ATGGAAAAAC CAAGACGATT 
CGGGTCACCA GGCCTGTCAA CCCGGCCCAA AGCAGGCCAT GCGAACGATG CTCGCCCCTC 
ACGCCCAACA TGACCAAAGA GCTCAGGGCA ATAGAAAACC TATCCTCTTC CTCCACTCCC 
AGGAAAGAGT CGCGGGATGA CAAACCCCTG ATTCCCCTGC TGTCACAGGA CTCTCTTCTC 
AGAGAAGGAG GCCACGCGGG ACTTCTCCTC AATGGGGTGG AGGATAACAA TGACCTGCTG 
AAAGAGATCG AGCCTGTGCT GAAGCTCATC AAGAACAGCA AAAAAGAGAT CAACGGAGAG 
GGCCAGAGGG TCATCGATAG GAAGGATGCA GAGGTGCAGG TGGAGAGAGA CCTTGGTGTG 
GGAAATGGAC AGAACGCCCA GCTGGCATCG GATAACGACA GAGTGTTTGA TGACCTCTGG 
AGAAAGGACA ACATGCCCAC GGTGCTTAAC AACCCTTACT CAGAAAGTGA CAAGCCACAG 
TCTTATGAAA GAGTTTCTCC TACCAAGGCT GTACAGAACG GAAGTCCCTC CAAATGTCCC 
AGATTCATGA AAGTGAAGAA TTGGGAAACG GGAGCCTTAT ACAATGATAC TTTACACCAC 
AGCTCCAGTA AGGTGCCGAT CTGCACTGAG CACGTTTGCA TAGGGTCTAT TATGGTGCCA 
AACCAGCACG CTCACAAACC AGATGAAGAC AGAAGAAAAG AGGAGCTGCT ACCACTTGCA 
ACTGATTTCA TCGACCAGTA CTACACATCA ATCAAAAGAT GTTGCTCAAA GGCCCATGTT 
GACCGGCTAG AAGAAGTCAA AAAAGAAATC GAAACTTCTG GAACATATCA GCTAAAGGAC 
ACAGAGCTAA TCTATGGGGC CAAGCATGCA TGGAGAAATG CAGCTCGCTG TGTGGGACGA 
ATCCAGTGGT CCAAATTACA GGTGTTTGAC GCCCGCGACT GCACAACCGC TCATGGAATG 
TTTAATTACA TTTGCAACCA CATCAAATAC GCCACCAACA AAGGAAACCT CAGATCAGCT 
ATTACCATCT TCCCCCAGAA GACAGATGGG AAGCATGACT TCCGGGTGTG GAACAGCCAG 
CTGATCCGCT ACGCAGGCTA CAAGCAGCCC GATGGATCAA TTCTAGGAGA TCCAGCTTCT 
GTAGAGCTTA CAGAGATCTG TATGCAGCAA GGATGGAAAG CTCCAAAAGG CCGGTTCGAT 
GTTTTGCCCC TTCTTCTCCA AGCCAATGGG AATGATCCAG AGCTTTTTGA GATCCCTGAT 
GATCTTGTAC TTGAGGTCCC CATCATACAT CCAAAGTTTG AATGGTTTAA GGAGCTGAAT 
CTAAAGTGGT ACGGCCTTCC TGCTGTGTCA AATATGCTTT TAGAGATCGG CGGTCTAGAG 
TTCACCGCAT GTCCCTTCAG CGGCTGGTAC ATGGGGACAG AAATTGGTGT GAGAGACTTC 
TGCGACAGCT CTCGATACAA CATACTTGAG GAAGTAGCTA CAATGATGGG TCTGGATACG 
AGGAAGACTT CGTCCCTGTG GAAGGACCAA GCACTGGTGG AGATCAACAT CGCAGTCCTT 
TATAGCTACC AGATGTCAAA GGTTACCATA GTAGACCATC ACTCGGCCAC TGAGTCCTTC 
ATGAAGCACA TGGAGAATGA GTACCGAGTG AGAGGAGGCT GTCCGGGAGA CTGGGTTTGG 
ATAGTGCCAC CCATGTCCGG TAGCATCACA CCTGTATTCC ACCAGGAAAT GCTCAACTAT 
CGCCTCACAC CATCTTTTGA GTACCAGCCT GACCCATGGA ATACTCACGT ATGGAAAGGA 
GTAAATGGGA CGCCAACAAA GAAAAGAGCG ATTGGATTCA AGAAACTTGC CAAGGCTGTA 
AAGTTCTCCG CCAAACTCAT GGGACAAGCC ATGGCCAAGA GAGTCAAAGC CACCATCTTA 
TTCGCCACAG AAACGGGGAA ATCGCAGGAC TATGCAAAAA CGCTCTGTGA GATTTTCAAG 
CACGCATTTG ATGCCAAGGT GATGTCTATG GATGAATATG ACACAGTGGA TCTGGAGCAC 
GAGACACTTG TGATAGTCGT GACAAGCACA TTCGGCAACG GTGACCCTCC TGAAAATGGA 
GAGAAATTTG GTGCGGCGCT GATGGAGATG AGACATCCCA CCACCAGCGT AGAGGACAGA 
AAGACGTACA AGGTGCGGTT TAACAGCGTT TCTTCGTATT CTGACACCCG CAAATCCTCC 
AGCGATGAGC CTGAATCAAA AGCCAACTTC GAGAGCACTG GACCGCTTGC TAATGTCAGA 
TTCTCCGTGT TTGGCCTTGG CTCTCGGGCA TACCCACACT TCTGTGCATT TGCCCACGCA 
GTGGACACGC TGTTCGAGGA GCTTGGCGGG GAACGAATCC TGCGCATGGG GGAAGGAGAC 
GAACTCTGCG GCCAGGAGGA ATCCTTCAGA ACGTGGGCTA AGAAAGTTTT TAAGGCTGCA 
TGCGACGTTT TTTGTGTCGG TGATGATGTT AACATCGAGA AAGCAAACGA TTCCCTGATT 
AGTAACGATC GCAGCTGGAA GAAAAGCAAG TTCCGGATGA CATACACGGC TGAAGCACCG 
ACACTAACGC AAGCGCTTTA CAGCATCCAT AAGAAGAAAG TTTACGGAGC CAAAGTTGTG 
CAGAGACAAA ACCTACAAAG TGCCAGATCA AACCGATTGA CGATATTTGT GCGTCTTGAT 
GCAAACAACC ACGAGAGCCT AAAATACCTG CCTGGAGACC ATTTAGGCGT TTTCCCTGGC 
AACAATGAAG ATCTTGTTAC CACTCTTATC GAGAAACTGG AGGATGCGCC GCCTGTCAAT 
CAAATAGTGA AAGTGGAGTT CTTGGAGGAA AGAAACACAG CTCTAGGAGT GATAAGCAAC 
TGGACAGATG AGAGTCGTAT TCCACCGTGC ACCATCTATC AGGCCTTCAA ATACTTTCTG 
GACATAACCA CCCCTCCTAG TCCTGTACTG TTGCAGCAGT TTGCTCCTCT AGCCACTAAC 
GAGAAACAGA GAAAGAGACT AGAGGTGCTC AGCAAGGGTT TGCAGGAGTA CGAGGAATGG 
AAGTGGTACA ATAATCCTAC CATGGTGGAG GTGCTGGAGG AGTTTCCATC CCTCCAGATC 
CCCTCCACCC TACTCTTGAC GCAGCTGCCT CTCCTACAGC CGCGCTACTA CTCCATCAGC 
TCCTCTCCAG ACCTCTATCC TGGAGAGATC CACCTCACTG TGGCTGTGGT GTCCTATCGA 
CCCAGAGATG GAGAAGGGCC TATTCATCAT GGAGTTTGTT CCTCATGGCT CAACAGCTTG 
GATGAAGGCG ATACAGTGCC GTGCTTTGTC AGAGGGGCTC CATCCTTCCG TATGCCAAAA 
GACAGCCAGG TTCCTTGCAT TCTGATTGGT CCTGGCACTG GAATCGCTCC ATTCAGAGCC 
TTCTGGCAAC AGAGACAGTA CGATGTCGAA AATAAAGGAA TCAAGGCTTG CCCCATGATC 
TTGGTGTTTG GTTGTCGGCA GTCCCAGATT GACCACATTT ACAAGGAACA GACCATCCAA 
GCCAAGAACA AGGAAGTGTT TAAAGAGCTT TACACGGCAT ACTCTAGAGA GCCCGGAAGA 
CCAAAGAAAT ACGTCCAGGA TGTGCTCCGC GAGCAGCTAT CTGAAACTGT GTACAAATGT 
CTAAGAGAAG AAGGAGGACA CATCTATGTC TGTGGGGACG TCACCATGGC CGGAGACGTC 
CTGAAAACTG TCCAGCAGAT CTTTAAACTG CACGGCAACA TGTCTCTGGA AGATGCGGGT 
TTCTATATCA GCAAACTACG GGATGAGAAT CGATACCATG AGGACATTTT TGGTGTAACC 
CTGCGCACTT ACGAGGTCAC CAACCGTCTG AGGTCAGAAT CCATTGCGTA CATCGAGGAG 
AGCAAGAAAG ATATAGATGA GGTGTTTTGC TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165110.1
CDS	238..4533
Translation	MQESEPSVCQLQPNIISVRLFKRKIGGLGFLVKQRVCKPPVIVSDLIRGGAAEECGLVQVGDIVLAVNNKPLVDMSYERALETLKNVSPESHAVLILRGPEGFTTHLETTLSSDGKTKTIRVTRPVNPAQSRPCERCSPLTPNMTKELRAIENLSSSSTPRKESRDDKPLIPLLSQDSLLREGGHAGLLLNGVEDNNDLLKEIEPVLKLIKNSKKEINGEGQRVIDRKDAEVQVERDLGVGNGQNAQLASDNDRVFDDLWRKDNMPTVLNNPYSESDKPQSYERVSPTKAVQNGSPSKCPRFMKVKNWETGALYNDTLHHSSSKVPICTEHVCIGSIMVPNQHAHKPDEDRRKEELLPLATDFIDQYYTSIKRCCSKAHVDRLEEVKKEIETSGTYQLKDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQKTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPASVELTEICMQQGWKAPKGRFDVLPLLLQANGNDPELFEIPDDLVLEVPIIHPKFEWFKELNLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDFCDSSRYNILEEVATMMGLDTRKTSSLWKDQALVEINIAVLYSYQMSKVTIVDHHSATESFMKHMENEYRVRGGCPGDWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILFATETGKSQDYAKTLCEIFKHAFDAKVMSMDEYDTVDLEHETLVIVVTSTFGNGDPPENGEKFGAALMEMRHPTTSVEDRKTYKVRFNSVSSYSDTRKSSSDEPESKANFESTGPLANVRFSVFGLGSRAYPHFCAFAHAVDTLFEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANDSLISNDRSWKKSKFRMTYTAEAPTLTQALYSIHKKKVYGAKVVQRQNLQSARSNRLTIFVRLDANNHESLKYLPGDHLGVFPGNNEDLVTTLIEKLEDAPPVNQIVKVEFLEERNTALGVISNWTDESRIPPCTIYQAFKYFLDITTPPSPVLLQQFAPLATNEKQRKRLEVLSKGLQEYEEWKWYNNPTMVEVLEEFPSLQIPSTLLLTQLPLLQPRYYSISSSPDLYPGEIHLTVAVVSYRPRDGEGPIHHGVCSSWLNSLDEGDTVPCFVRGAPSFRMPKDSQVPCILIGPGTGIAPFRAFWQQRQYDVENKGIKACPMILVFGCRQSQIDHIYKEQTIQAKNKEVFKELYTAYSREPGRPKKYVQDVLREQLSETVYKCLREEGGHIYVCGDVTMAGDVLKTVQQIFKLHGNMSLEDAGFYISKLRDENRYHEDIFGVTLRTYEVTNRLRSESIAYIEESKKDIDEVFCS

Target ORF information:

RefSeq Version	XM_005165053.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nitric oxide synthase 1 (neuronal) (nos1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165053.4

ORF Insert Sequence:

ATGCAGGAAT CAGAGCCCAG CGTGTGTCAA CTACAGCCCA ACATCATCTC AGTTCGCCTC 
TTTAAAAGAA AGATCGGTGG ACTGGGCTTT CTGGTCAAGC AGCGGGTGTG CAAACCACCT 
GTCATCGTCT CGGACCTCAT CCGGGGAGGA GCGGCGGAGG AATGCGGGCT TGTCCAAGTG 
GGCGACATCG TCCTTGCTGT AAACAACAAG CCGTTGGTGG ACATGAGTTA TGAACGTGCT 
CTTGAAACCC TGAAGAACGT GTCACCCGAA AGCCACGCTG TCCTGATCCT TCGTGGACCA 
GAGGGTTTCA CCACTCACCT GGAGACCACG CTGAGCAGTG ATGGAAAAAC CAAGACGATT 
CGGGTCACCA GGCCTGTCAA CCCGGCCCAA AGCAGGCCAT GCGAACGATG CTCGCCCCTC 
ACGCCCAACA TGACCAAAGA GCTCAGGGCA ATAGAAAACC TATCCTCTTC CTCCACTCCC 
AGGAAAGAGT CGCGGGATGA CAAACCCCTG ATTCCCCTGC TGTCACAGGA CTCTCTTCTC 
AGAGAAGGAG GCCACGCGGG ACTTCTCCTC AATGGGGTGG AGGATAACAA TGACCTGCTG 
AAAGAGATCG AGCCTGTGCT GAAGCTCATC AAGAACAGCA AAAAAGAGAT CAACGGAGAG 
GGCCAGAGGG TCATCGATAG GAAGGATGCA GAGGTGCAGG TGGAGAGAGA CCTTGGTGTG 
GGAAATGGAC AGAACGCCCA GCTGGCATCG GATAACGACA GAGTGTTTGA TGACCTCTGG 
AGAAAGGACA ACATGCCCAC GGTGCTTAAC AACCCTTACT CAGAAAGTGA CAAGCCACAG 
TCTTATGAAA GAGTTTCTCC TACCAAGGCT GTACAGAACG GAAGTCCCTC CAAATGTCCC 
AGATTCATGA AAGTGAAGAA TTGGGAAACG GGAGCCTTAT ACAATGATAC TTTACACCAC 
AGCTCCAGTA AGGTGCCGAT CTGCACTGAG CACGTTTGCA TAGGGTCTAT TATGGTGCCA 
AACCAGCACG CTCACAAACC AGATGAAGAC AGAAGAAAAG AGGAGCTGCT ACCACTTGCA 
ACTGATTTCA TCGACCAGTA CTACACATCA ATCAAAAGAT GTTGCTCAAA GGCCCATGTT 
GACCGGCTAG AAGAAGTCAA AAAAGAAATC GAAACTTCTG GAACATATCA GCTAAAGGAC 
ACAGAGCTAA TCTATGGGGC CAAGCATGCA TGGAGAAATG CAGCTCGCTG TGTGGGACGA 
ATCCAGTGGT CCAAATTACA GGTGTTTGAC GCCCGCGACT GCACAACCGC TCATGGAATG 
TTTAATTACA TTTGCAACCA CATCAAATAC GCCACCAACA AAGGAAACCT CAGATCAGCT 
ATTACCATCT TCCCCCAGAA GACAGATGGG AAGCATGACT TCCGGGTGTG GAACAGCCAG 
CTGATCCGCT ACGCAGGCTA CAAGCAGCCC GATGGATCAA TTCTAGGAGA TCCAGCTTCT 
GTAGAGCTTA CAGAGATCTG TATGCAGCAA GGATGGAAAG CTCCAAAAGG CCGGTTCGAT 
GTTTTGCCCC TTCTTCTCCA AGCCAATGGG AATGATCCAG AGCTTTTTGA GATCCCTGAT 
GATCTTGTAC TTGAGGTCCC CATCATACAT CCAAAGTTTG AATGGTTTAA GGAGCTGAAT 
CTAAAGTGGT ACGGCCTTCC TGCTGTGTCA AATATGCTTT TAGAGATCGG CGGTCTAGAG 
TTCACCGCAT GTCCCTTCAG CGGCTGGTAC ATGGGGACAG AAATTGGTGT GAGAGACTTC 
TGCGACAGCT CTCGATACAA CATACTTGAG GAAGTAGCTA CAATGATGGG TCTGGATACG 
AGGAAGACTT CGTCCCTGTG GAAGGACCAA GCACTGGTGG AGATCAACAT CGCAGTCCTT 
TATAGCTACC AGATGTCAAA GGTTACCATA GTAGACCATC ACTCGGCCAC TGAGTCCTTC 
ATGAAGCACA TGGAGAATGA GTACCGAGTG AGAGGAGGCT GTCCGGGAGA CTGGGTTTGG 
ATAGTGCCAC CCATGTCCGG TAGCATCACA CCTGTATTCC ACCAGGAAAT GCTCAACTAT 
CGCCTCACAC CATCTTTTGA GTACCAGCCT GACCCATGGA ATACTCACGT ATGGAAAGGA 
GTAAATGGGA CGCCAACAAA GAAAAGAGCG ATTGGATTCA AGAAACTTGC CAAGGCTGTA 
AAGTTCTCCG CCAAACTCAT GGGACAAGCC ATGGCCAAGA GAGTCAAAGC CACCATCTTA 
TTCGCCACAG AAACGGGGAA ATCGCAGGAC TATGCAAAAA CGCTCTGTGA GATTTTCAAG 
CACGCATTTG ATGCCAAGGT GATGTCTATG GATGAATATG ACACAGTGGA TCTGGAGCAC 
GAGACACTTG TGATAGTCGT GACAAGCACA TTCGGCAACG GTGACCCTCC TGAAAATGGA 
GAGAAATTTG GTGCGGCGCT GATGGAGATG AGACATCCCA CCACCAGCGT AGAGGACAGA 
AAGACGTACA AGGTGCGGTT TAACAGCGTT TCTTCGTATT CTGACACCCG CAAATCCTCC 
AGCGATGAGC CTGAATCAAA AGCCAACTTC GAGAGCACTG GACCGCTTGC TAATGTCAGA 
TTCTCCGTGT TTGGCCTTGG CTCTCGGGCA TACCCACACT TCTGTGCATT TGCCCACGCA 
GTGGACACGC TGTTCGAGGA GCTTGGCGGG GAACGAATCC TGCGCATGGG GGAAGGAGAC 
GAACTCTGCG GCCAGGAGGA ATCCTTCAGA ACGTGGGCTA AGAAAGTTTT TAAGGCTGCA 
TGCGACGTTT TTTGTGTCGG TGATGATGTT AACATCGAGA AAGCAAACGA TTCCCTGATT 
AGTAACGATC GCAGCTGGAA GAAAAGCAAG TTCCGGATGA CATACACGGC TGAAGCACCG 
ACACTAACGC AAGCGCTTTA CAGCATCCAT AAGAAGAAAG TTTACGGAGC CAAAGTTGTG 
CAGAGACAAA ACCTACAAAG TGCCAGATCA AACCGATTGA CGATATTTGT GCGTCTTGAT 
GCAAACAACC ACGAGAGCCT AAAATACCTG CCTGGAGACC ATTTAGGCGT TTTCCCTGGC 
AACAATGAAG ATCTTGTTAC CACTCTTATC GAGAAACTGG AGGATGCGCC GCCTGTCAAT 
CAAATAGTGA AAGTGGAGTT CTTGGAGGAA AGAAACACAG CTCTAGGAGT GATAAGCAAC 
TGGACAGATG AGAGTCGTAT TCCACCGTGC ACCATCTATC AGGCCTTCAA ATACTTTCTG 
GACATAACCA CCCCTCCTAG TCCTGTACTG TTGCAGCAGT TTGCTCCTCT AGCCACTAAC 
GAGAAACAGA GAAAGAGACT AGAGGTGCTC AGCAAGGGTT TGCAGGAGTA CGAGGAATGG 
AAGTGGTACA ATAATCCTAC CATGGTGGAG GTGCTGGAGG AGTTTCCATC CCTCCAGATC 
CCCTCCACCC TACTCTTGAC GCAGCTGCCT CTCCTACAGC CGCGCTACTA CTCCATCAGC 
TCCTCTCCAG ACCTCTATCC TGGAGAGATC CACCTCACTG TGGCTGTGGT GTCCTATCGA 
CCCAGAGATG GAGAAGGGCC TATTCATCAT GGAGTTTGTT CCTCATGGCT CAACAGCTTG 
GATGAAGGCG ATACAGTGCC GTGCTTTGTC AGAGGGGCTC CATCCTTCCG TATGCCAAAA 
GACAGCCAGG TTCCTTGCAT TCTGATTGGT CCTGGCACTG GAATCGCTCC ATTCAGAGCC 
TTCTGGCAAC AGAGACAGTA CGATGTCGAA AATAAAGGAA TCAAGGCTTG CCCCATGATC 
TTGGTGTTTG GTTGTCGGCA GTCCCAGATT GACCACATTT ACAAGGAACA GACCATCCAA 
GCCAAGAACA AGGAAGTGTT TAAAGAGCTT TACACGGCAT ACTCTAGAGA GCCCGGAAGA 
CCAAAGAAAT ACGTCCAGGA TGTGCTCCGC GAGCAGCTAT CTGAAACTGT GTACAAATGT 
CTAAGAGAAG AAGGAGGACA CATCTATGTC TGTGGGGACG TCACCATGGC CGGAGACGTC 
CTGAAAACTG TCCAGCAGAT CTTTAAACTG CACGGCAACA TGTCTCTGGA AGATGCGGGT 
TTCTATATCA GCAAACTACG GGATGAGAAT CGATACCATG AGGACATTTT TGGTGTAACC 
CTGCGCACTT ACGAGGTCAC CAACCGTCTG AGGTCAGAAT CCATTGCGTA CATCGAGGAG 
AGCAAGAAAG ATATAGATGA GGTGTTTTGC TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_005165053.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165110.1 Danio rerio nitric oxide synthase 1 (neuronal) (nos1), transcript variant X1, mRNA.

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CloneID	ODa39410
Clone ID Related Accession (Same CDS sequence)	XM_005165053.4 , XM_005165053.3
Accession Version	XM_005165053.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	nitric oxide synthase, brain isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165053.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAT CAGAGCCCAG CGTGTGTCAA CTACAGCCCA ACATCATCTC AGTTCGCCTC 
TTTAAAAGAA AGATCGGTGG ACTGGGCTTT CTGGTCAAGC AGCGGGTGTG CAAACCACCT 
GTCATCGTCT CGGACCTCAT CCGGGGAGGA GCGGCGGAGG AATGCGGGCT TGTCCAAGTG 
GGCGACATCG TCCTTGCTGT AAACAACAAG CCGTTGGTGG ACATGAGTTA TGAACGTGCT 
CTTGAAACCC TGAAGAACGT GTCACCCGAA AGCCACGCTG TCCTGATCCT TCGTGGACCA 
GAGGGTTTCA CCACTCACCT GGAGACCACG CTGAGCAGTG ATGGAAAAAC CAAGACGATT 
CGGGTCACCA GGCCTGTCAA CCCGGCCCAA AGCAGGCCAT GCGAACGATG CTCGCCCCTC 
ACGCCCAACA TGACCAAAGA GCTCAGGGCA ATAGAAAACC TATCCTCTTC CTCCACTCCC 
AGGAAAGAGT CGCGGGATGA CAAACCCCTG ATTCCCCTGC TGTCACAGGA CTCTCTTCTC 
AGAGAAGGAG GCCACGCGGG ACTTCTCCTC AATGGGGTGG AGGATAACAA TGACCTGCTG 
AAAGAGATCG AGCCTGTGCT GAAGCTCATC AAGAACAGCA AAAAAGAGAT CAACGGAGAG 
GGCCAGAGGG TCATCGATAG GAAGGATGCA GAGGTGCAGG TGGAGAGAGA CCTTGGTGTG 
GGAAATGGAC AGAACGCCCA GCTGGCATCG GATAACGACA GAGTGTTTGA TGACCTCTGG 
AGAAAGGACA ACATGCCCAC GGTGCTTAAC AACCCTTACT CAGAAAGTGA CAAGCCACAG 
TCTTATGAAA GAGTTTCTCC TACCAAGGCT GTACAGAACG GAAGTCCCTC CAAATGTCCC 
AGATTCATGA AAGTGAAGAA TTGGGAAACG GGAGCCTTAT ACAATGATAC TTTACACCAC 
AGCTCCAGTA AGGTGCCGAT CTGCACTGAG CACGTTTGCA TAGGGTCTAT TATGGTGCCA 
AACCAGCACG CTCACAAACC AGATGAAGAC AGAAGAAAAG AGGAGCTGCT ACCACTTGCA 
ACTGATTTCA TCGACCAGTA CTACACATCA ATCAAAAGAT GTTGCTCAAA GGCCCATGTT 
GACCGGCTAG AAGAAGTCAA AAAAGAAATC GAAACTTCTG GAACATATCA GCTAAAGGAC 
ACAGAGCTAA TCTATGGGGC CAAGCATGCA TGGAGAAATG CAGCTCGCTG TGTGGGACGA 
ATCCAGTGGT CCAAATTACA GGTGTTTGAC GCCCGCGACT GCACAACCGC TCATGGAATG 
TTTAATTACA TTTGCAACCA CATCAAATAC GCCACCAACA AAGGAAACCT CAGATCAGCT 
ATTACCATCT TCCCCCAGAA GACAGATGGG AAGCATGACT TCCGGGTGTG GAACAGCCAG 
CTGATCCGCT ACGCAGGCTA CAAGCAGCCC GATGGATCAA TTCTAGGAGA TCCAGCTTCT 
GTAGAGCTTA CAGAGATCTG TATGCAGCAA GGATGGAAAG CTCCAAAAGG CCGGTTCGAT 
GTTTTGCCCC TTCTTCTCCA AGCCAATGGG AATGATCCAG AGCTTTTTGA GATCCCTGAT 
GATCTTGTAC TTGAGGTCCC CATCATACAT CCAAAGTTTG AATGGTTTAA GGAGCTGAAT 
CTAAAGTGGT ACGGCCTTCC TGCTGTGTCA AATATGCTTT TAGAGATCGG CGGTCTAGAG 
TTCACCGCAT GTCCCTTCAG CGGCTGGTAC ATGGGGACAG AAATTGGTGT GAGAGACTTC 
TGCGACAGCT CTCGATACAA CATACTTGAG GAAGTAGCTA CAATGATGGG TCTGGATACG 
AGGAAGACTT CGTCCCTGTG GAAGGACCAA GCACTGGTGG AGATCAACAT CGCAGTCCTT 
TATAGCTACC AGATGTCAAA GGTTACCATA GTAGACCATC ACTCGGCCAC TGAGTCCTTC 
ATGAAGCACA TGGAGAATGA GTACCGAGTG AGAGGAGGCT GTCCGGGAGA CTGGGTTTGG 
ATAGTGCCAC CCATGTCCGG TAGCATCACA CCTGTATTCC ACCAGGAAAT GCTCAACTAT 
CGCCTCACAC CATCTTTTGA GTACCAGCCT GACCCATGGA ATACTCACGT ATGGAAAGGA 
GTAAATGGGA CGCCAACAAA GAAAAGAGCG ATTGGATTCA AGAAACTTGC CAAGGCTGTA 
AAGTTCTCCG CCAAACTCAT GGGACAAGCC ATGGCCAAGA GAGTCAAAGC CACCATCTTA 
TTCGCCACAG AAACGGGGAA ATCGCAGGAC TATGCAAAAA CGCTCTGTGA GATTTTCAAG 
CACGCATTTG ATGCCAAGGT GATGTCTATG GATGAATATG ACACAGTGGA TCTGGAGCAC 
GAGACACTTG TGATAGTCGT GACAAGCACA TTCGGCAACG GTGACCCTCC TGAAAATGGA 
GAGAAATTTG GTGCGGCGCT GATGGAGATG AGACATCCCA CCACCAGCGT AGAGGACAGA 
AAGACGTACA AGGTGCGGTT TAACAGCGTT TCTTCGTATT CTGACACCCG CAAATCCTCC 
AGCGATGAGC CTGAATCAAA AGCCAACTTC GAGAGCACTG GACCGCTTGC TAATGTCAGA 
TTCTCCGTGT TTGGCCTTGG CTCTCGGGCA TACCCACACT TCTGTGCATT TGCCCACGCA 
GTGGACACGC TGTTCGAGGA GCTTGGCGGG GAACGAATCC TGCGCATGGG GGAAGGAGAC 
GAACTCTGCG GCCAGGAGGA ATCCTTCAGA ACGTGGGCTA AGAAAGTTTT TAAGGCTGCA 
TGCGACGTTT TTTGTGTCGG TGATGATGTT AACATCGAGA AAGCAAACGA TTCCCTGATT 
AGTAACGATC GCAGCTGGAA GAAAAGCAAG TTCCGGATGA CATACACGGC TGAAGCACCG 
ACACTAACGC AAGCGCTTTA CAGCATCCAT AAGAAGAAAG TTTACGGAGC CAAAGTTGTG 
CAGAGACAAA ACCTACAAAG TGCCAGATCA AACCGATTGA CGATATTTGT GCGTCTTGAT 
GCAAACAACC ACGAGAGCCT AAAATACCTG CCTGGAGACC ATTTAGGCGT TTTCCCTGGC 
AACAATGAAG ATCTTGTTAC CACTCTTATC GAGAAACTGG AGGATGCGCC GCCTGTCAAT 
CAAATAGTGA AAGTGGAGTT CTTGGAGGAA AGAAACACAG CTCTAGGAGT GATAAGCAAC 
TGGACAGATG AGAGTCGTAT TCCACCGTGC ACCATCTATC AGGCCTTCAA ATACTTTCTG 
GACATAACCA CCCCTCCTAG TCCTGTACTG TTGCAGCAGT TTGCTCCTCT AGCCACTAAC 
GAGAAACAGA GAAAGAGACT AGAGGTGCTC AGCAAGGGTT TGCAGGAGTA CGAGGAATGG 
AAGTGGTACA ATAATCCTAC CATGGTGGAG GTGCTGGAGG AGTTTCCATC CCTCCAGATC 
CCCTCCACCC TACTCTTGAC GCAGCTGCCT CTCCTACAGC CGCGCTACTA CTCCATCAGC 
TCCTCTCCAG ACCTCTATCC TGGAGAGATC CACCTCACTG TGGCTGTGGT GTCCTATCGA 
CCCAGAGATG GAGAAGGGCC TATTCATCAT GGAGTTTGTT CCTCATGGCT CAACAGCTTG 
GATGAAGGCG ATACAGTGCC GTGCTTTGTC AGAGGGGCTC CATCCTTCCG TATGCCAAAA 
GACAGCCAGG TTCCTTGCAT TCTGATTGGT CCTGGCACTG GAATCGCTCC ATTCAGAGCC 
TTCTGGCAAC AGAGACAGTA CGATGTCGAA AATAAAGGAA TCAAGGCTTG CCCCATGATC 
TTGGTGTTTG GTTGTCGGCA GTCCCAGATT GACCACATTT ACAAGGAACA GACCATCCAA 
GCCAAGAACA AGGAAGTGTT TAAAGAGCTT TACACGGCAT ACTCTAGAGA GCCCGGAAGA 
CCAAAGAAAT ACGTCCAGGA TGTGCTCCGC GAGCAGCTAT CTGAAACTGT GTACAAATGT 
CTAAGAGAAG AAGGAGGACA CATCTATGTC TGTGGGGACG TCACCATGGC CGGAGACGTC 
CTGAAAACTG TCCAGCAGAT CTTTAAACTG CACGGCAACA TGTCTCTGGA AGATGCGGGT 
TTCTATATCA GCAAACTACG GGATGAGAAT CGATACCATG AGGACATTTT TGGTGTAACC 
CTGCGCACTT ACGAGGTCAC CAACCGTCTG AGGTCAGAAT CCATTGCGTA CATCGAGGAG 
AGCAAGAAAG ATATAGATGA GGTGTTTTGC TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165110.1
CDS	209..4504
Translation	MQESEPSVCQLQPNIISVRLFKRKIGGLGFLVKQRVCKPPVIVSDLIRGGAAEECGLVQVGDIVLAVNNKPLVDMSYERALETLKNVSPESHAVLILRGPEGFTTHLETTLSSDGKTKTIRVTRPVNPAQSRPCERCSPLTPNMTKELRAIENLSSSSTPRKESRDDKPLIPLLSQDSLLREGGHAGLLLNGVEDNNDLLKEIEPVLKLIKNSKKEINGEGQRVIDRKDAEVQVERDLGVGNGQNAQLASDNDRVFDDLWRKDNMPTVLNNPYSESDKPQSYERVSPTKAVQNGSPSKCPRFMKVKNWETGALYNDTLHHSSSKVPICTEHVCIGSIMVPNQHAHKPDEDRRKEELLPLATDFIDQYYTSIKRCCSKAHVDRLEEVKKEIETSGTYQLKDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQKTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPASVELTEICMQQGWKAPKGRFDVLPLLLQANGNDPELFEIPDDLVLEVPIIHPKFEWFKELNLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDFCDSSRYNILEEVATMMGLDTRKTSSLWKDQALVEINIAVLYSYQMSKVTIVDHHSATESFMKHMENEYRVRGGCPGDWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILFATETGKSQDYAKTLCEIFKHAFDAKVMSMDEYDTVDLEHETLVIVVTSTFGNGDPPENGEKFGAALMEMRHPTTSVEDRKTYKVRFNSVSSYSDTRKSSSDEPESKANFESTGPLANVRFSVFGLGSRAYPHFCAFAHAVDTLFEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANDSLISNDRSWKKSKFRMTYTAEAPTLTQALYSIHKKKVYGAKVVQRQNLQSARSNRLTIFVRLDANNHESLKYLPGDHLGVFPGNNEDLVTTLIEKLEDAPPVNQIVKVEFLEERNTALGVISNWTDESRIPPCTIYQAFKYFLDITTPPSPVLLQQFAPLATNEKQRKRLEVLSKGLQEYEEWKWYNNPTMVEVLEEFPSLQIPSTLLLTQLPLLQPRYYSISSSPDLYPGEIHLTVAVVSYRPRDGEGPIHHGVCSSWLNSLDEGDTVPCFVRGAPSFRMPKDSQVPCILIGPGTGIAPFRAFWQQRQYDVENKGIKACPMILVFGCRQSQIDHIYKEQTIQAKNKEVFKELYTAYSREPGRPKKYVQDVLREQLSETVYKCLREEGGHIYVCGDVTMAGDVLKTVQQIFKLHGNMSLEDAGFYISKLRDENRYHEDIFGVTLRTYEVTNRLRSESIAYIEESKKDIDEVFCS

Target ORF information:

RefSeq Version	XM_005165053.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nitric oxide synthase 1 (neuronal) (nos1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165053.3

ORF Insert Sequence:

ATGCAGGAAT CAGAGCCCAG CGTGTGTCAA CTACAGCCCA ACATCATCTC AGTTCGCCTC 
TTTAAAAGAA AGATCGGTGG ACTGGGCTTT CTGGTCAAGC AGCGGGTGTG CAAACCACCT 
GTCATCGTCT CGGACCTCAT CCGGGGAGGA GCGGCGGAGG AATGCGGGCT TGTCCAAGTG 
GGCGACATCG TCCTTGCTGT AAACAACAAG CCGTTGGTGG ACATGAGTTA TGAACGTGCT 
CTTGAAACCC TGAAGAACGT GTCACCCGAA AGCCACGCTG TCCTGATCCT TCGTGGACCA 
GAGGGTTTCA CCACTCACCT GGAGACCACG CTGAGCAGTG ATGGAAAAAC CAAGACGATT 
CGGGTCACCA GGCCTGTCAA CCCGGCCCAA AGCAGGCCAT GCGAACGATG CTCGCCCCTC 
ACGCCCAACA TGACCAAAGA GCTCAGGGCA ATAGAAAACC TATCCTCTTC CTCCACTCCC 
AGGAAAGAGT CGCGGGATGA CAAACCCCTG ATTCCCCTGC TGTCACAGGA CTCTCTTCTC 
AGAGAAGGAG GCCACGCGGG ACTTCTCCTC AATGGGGTGG AGGATAACAA TGACCTGCTG 
AAAGAGATCG AGCCTGTGCT GAAGCTCATC AAGAACAGCA AAAAAGAGAT CAACGGAGAG 
GGCCAGAGGG TCATCGATAG GAAGGATGCA GAGGTGCAGG TGGAGAGAGA CCTTGGTGTG 
GGAAATGGAC AGAACGCCCA GCTGGCATCG GATAACGACA GAGTGTTTGA TGACCTCTGG 
AGAAAGGACA ACATGCCCAC GGTGCTTAAC AACCCTTACT CAGAAAGTGA CAAGCCACAG 
TCTTATGAAA GAGTTTCTCC TACCAAGGCT GTACAGAACG GAAGTCCCTC CAAATGTCCC 
AGATTCATGA AAGTGAAGAA TTGGGAAACG GGAGCCTTAT ACAATGATAC TTTACACCAC 
AGCTCCAGTA AGGTGCCGAT CTGCACTGAG CACGTTTGCA TAGGGTCTAT TATGGTGCCA 
AACCAGCACG CTCACAAACC AGATGAAGAC AGAAGAAAAG AGGAGCTGCT ACCACTTGCA 
ACTGATTTCA TCGACCAGTA CTACACATCA ATCAAAAGAT GTTGCTCAAA GGCCCATGTT 
GACCGGCTAG AAGAAGTCAA AAAAGAAATC GAAACTTCTG GAACATATCA GCTAAAGGAC 
ACAGAGCTAA TCTATGGGGC CAAGCATGCA TGGAGAAATG CAGCTCGCTG TGTGGGACGA 
ATCCAGTGGT CCAAATTACA GGTGTTTGAC GCCCGCGACT GCACAACCGC TCATGGAATG 
TTTAATTACA TTTGCAACCA CATCAAATAC GCCACCAACA AAGGAAACCT CAGATCAGCT 
ATTACCATCT TCCCCCAGAA GACAGATGGG AAGCATGACT TCCGGGTGTG GAACAGCCAG 
CTGATCCGCT ACGCAGGCTA CAAGCAGCCC GATGGATCAA TTCTAGGAGA TCCAGCTTCT 
GTAGAGCTTA CAGAGATCTG TATGCAGCAA GGATGGAAAG CTCCAAAAGG CCGGTTCGAT 
GTTTTGCCCC TTCTTCTCCA AGCCAATGGG AATGATCCAG AGCTTTTTGA GATCCCTGAT 
GATCTTGTAC TTGAGGTCCC CATCATACAT CCAAAGTTTG AATGGTTTAA GGAGCTGAAT 
CTAAAGTGGT ACGGCCTTCC TGCTGTGTCA AATATGCTTT TAGAGATCGG CGGTCTAGAG 
TTCACCGCAT GTCCCTTCAG CGGCTGGTAC ATGGGGACAG AAATTGGTGT GAGAGACTTC 
TGCGACAGCT CTCGATACAA CATACTTGAG GAAGTAGCTA CAATGATGGG TCTGGATACG 
AGGAAGACTT CGTCCCTGTG GAAGGACCAA GCACTGGTGG AGATCAACAT CGCAGTCCTT 
TATAGCTACC AGATGTCAAA GGTTACCATA GTAGACCATC ACTCGGCCAC TGAGTCCTTC 
ATGAAGCACA TGGAGAATGA GTACCGAGTG AGAGGAGGCT GTCCGGGAGA CTGGGTTTGG 
ATAGTGCCAC CCATGTCCGG TAGCATCACA CCTGTATTCC ACCAGGAAAT GCTCAACTAT 
CGCCTCACAC CATCTTTTGA GTACCAGCCT GACCCATGGA ATACTCACGT ATGGAAAGGA 
GTAAATGGGA CGCCAACAAA GAAAAGAGCG ATTGGATTCA AGAAACTTGC CAAGGCTGTA 
AAGTTCTCCG CCAAACTCAT GGGACAAGCC ATGGCCAAGA GAGTCAAAGC CACCATCTTA 
TTCGCCACAG AAACGGGGAA ATCGCAGGAC TATGCAAAAA CGCTCTGTGA GATTTTCAAG 
CACGCATTTG ATGCCAAGGT GATGTCTATG GATGAATATG ACACAGTGGA TCTGGAGCAC 
GAGACACTTG TGATAGTCGT GACAAGCACA TTCGGCAACG GTGACCCTCC TGAAAATGGA 
GAGAAATTTG GTGCGGCGCT GATGGAGATG AGACATCCCA CCACCAGCGT AGAGGACAGA 
AAGACGTACA AGGTGCGGTT TAACAGCGTT TCTTCGTATT CTGACACCCG CAAATCCTCC 
AGCGATGAGC CTGAATCAAA AGCCAACTTC GAGAGCACTG GACCGCTTGC TAATGTCAGA 
TTCTCCGTGT TTGGCCTTGG CTCTCGGGCA TACCCACACT TCTGTGCATT TGCCCACGCA 
GTGGACACGC TGTTCGAGGA GCTTGGCGGG GAACGAATCC TGCGCATGGG GGAAGGAGAC 
GAACTCTGCG GCCAGGAGGA ATCCTTCAGA ACGTGGGCTA AGAAAGTTTT TAAGGCTGCA 
TGCGACGTTT TTTGTGTCGG TGATGATGTT AACATCGAGA AAGCAAACGA TTCCCTGATT 
AGTAACGATC GCAGCTGGAA GAAAAGCAAG TTCCGGATGA CATACACGGC TGAAGCACCG 
ACACTAACGC AAGCGCTTTA CAGCATCCAT AAGAAGAAAG TTTACGGAGC CAAAGTTGTG 
CAGAGACAAA ACCTACAAAG TGCCAGATCA AACCGATTGA CGATATTTGT GCGTCTTGAT 
GCAAACAACC ACGAGAGCCT AAAATACCTG CCTGGAGACC ATTTAGGCGT TTTCCCTGGC 
AACAATGAAG ATCTTGTTAC CACTCTTATC GAGAAACTGG AGGATGCGCC GCCTGTCAAT 
CAAATAGTGA AAGTGGAGTT CTTGGAGGAA AGAAACACAG CTCTAGGAGT GATAAGCAAC 
TGGACAGATG AGAGTCGTAT TCCACCGTGC ACCATCTATC AGGCCTTCAA ATACTTTCTG 
GACATAACCA CCCCTCCTAG TCCTGTACTG TTGCAGCAGT TTGCTCCTCT AGCCACTAAC 
GAGAAACAGA GAAAGAGACT AGAGGTGCTC AGCAAGGGTT TGCAGGAGTA CGAGGAATGG 
AAGTGGTACA ATAATCCTAC CATGGTGGAG GTGCTGGAGG AGTTTCCATC CCTCCAGATC 
CCCTCCACCC TACTCTTGAC GCAGCTGCCT CTCCTACAGC CGCGCTACTA CTCCATCAGC 
TCCTCTCCAG ACCTCTATCC TGGAGAGATC CACCTCACTG TGGCTGTGGT GTCCTATCGA 
CCCAGAGATG GAGAAGGGCC TATTCATCAT GGAGTTTGTT CCTCATGGCT CAACAGCTTG 
GATGAAGGCG ATACAGTGCC GTGCTTTGTC AGAGGGGCTC CATCCTTCCG TATGCCAAAA 
GACAGCCAGG TTCCTTGCAT TCTGATTGGT CCTGGCACTG GAATCGCTCC ATTCAGAGCC 
TTCTGGCAAC AGAGACAGTA CGATGTCGAA AATAAAGGAA TCAAGGCTTG CCCCATGATC 
TTGGTGTTTG GTTGTCGGCA GTCCCAGATT GACCACATTT ACAAGGAACA GACCATCCAA 
GCCAAGAACA AGGAAGTGTT TAAAGAGCTT TACACGGCAT ACTCTAGAGA GCCCGGAAGA 
CCAAAGAAAT ACGTCCAGGA TGTGCTCCGC GAGCAGCTAT CTGAAACTGT GTACAAATGT 
CTAAGAGAAG AAGGAGGACA CATCTATGTC TGTGGGGACG TCACCATGGC CGGAGACGTC 
CTGAAAACTG TCCAGCAGAT CTTTAAACTG CACGGCAACA TGTCTCTGGA AGATGCGGGT 
TTCTATATCA GCAAACTACG GGATGAGAAT CGATACCATG AGGACATTTT TGGTGTAACC 
CTGCGCACTT ACGAGGTCAC CAACCGTCTG AGGTCAGAAT CCATTGCGTA CATCGAGGAG 
AGCAAGAAAG ATATAGATGA GGTGTTTTGC TCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nitric oxide interacts with monoamine oxidase to modulate aggression and anxiety-like behaviour.
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology3030-43(2020 Jan)
Carreño Gutiérrez H,O'Leary A,Freudenberg F,Fedele G,Wilkinson R,Markham E,van Eeden F,Reif A,Norton WHJ

Early development of the enteric nervous system visualized by using a new transgenic zebrafish line harboring a regulatory region for choline acetyltransferase a (chata) gene.
Gene expression patterns : GEP2812-21(2018 06)
Nikaido M,Izumi S,Ohnuki H,Takigawa Y,Yamasu K,Hatta K

The dual role of cGMP in oocyte maturation of zebrafish.
Biochemical and biophysical research communications499(4)998-1003(2018 05)
Li J,Zhou W,Wang Y,Niu C

Histone acetyltransferase 7 (KAT7)-dependent intragenic histone acetylation regulates endothelial cell gene regulation.
The Journal of biological chemistry293(12)4381-4402(2018 03)
Yan MS,Turgeon PJ,Man HJ,Dubinsky MK,Ho JJD,El-Rass S,Wang YD,Wen XY,Marsden PA

Mycobacterium marinum Infection in Zebrafish and Microglia Imitates the Early Stage of Tuberculous Meningitis.
Journal of molecular neuroscience : MN64(2)321-330(2018 Feb)
Chen Z,Shao XY,Wang C,Hua MH,Wang CN,Wang X,Wang QJ,Yao JY,Fan YH,Qin YW

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NOS1 cDNA ORF clone, Danio rerio(Zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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