NOS1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS1
Entrez Gene ID	427721
Full Name	nitric oxide synthase 1
General protein information	Preferred Names nitric oxide synthase 1 Names nitric oxide synthase, brain nitric oxide synthase 1 (neuronal)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015294873.2	XP_015150359.2	nitric oxide synthase, brain isoform X1
XM_015294875.2	XP_015150361.2	nitric oxide synthase, brain isoform X2
XM_015294873.1	XP_015150359.1	nitric oxide synthase, brain isoform X1
XM_015294874.1	XP_015150360.1	nitric oxide synthase, brain isoform X1
XM_015294874.2	XP_015150360.2	nitric oxide synthase, brain isoform X1
XM_015294875.1	XP_015150361.1	nitric oxide synthase, brain isoform X2
XM_004934480.3	XP_004934537.3	nitric oxide synthase, brain isoform X1
XM_004934480.2	XP_004934537.2	nitric oxide synthase, brain isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NOS1	XP_003314004.2
Rattus norvegicus (Norway rat)	Nos1	NP_434686.1
Gallus gallus (chicken)	NOS1	XP_004934537.1
Xenopus tropicalis (tropical clawed frog)	nos1	XP_004910558.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008255	XP_317213.1
Macaca mulatta (Rhesus monkey)	NOS1	XP_001083352.2
Mus musculus (house mouse)	Nos1	NP_032738.1
Homo sapiens (human)	NOS1	NP_000611.1
Bos taurus (cattle)	NOS1	XP_002694631.3
Canis lupus familiaris (dog)	NOS1	NP_001182074.1
Danio rerio (zebrafish)	nos1	NP_571735.1
Drosophila melanogaster (fruit fly)	Nos	NP_523541.2

Gene Ontology

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa144742	XM_015294875.2 Latest version!	Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa15889	XM_004934480.3 Latest version!	Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa15889	XM_015294874.2 Latest version!	Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa15889	XM_015294873.2 Latest version!	Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa43086	XM_004934480.2 Latest version!	Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa43086	XM_015294874.1 Latest version!	Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa43086	XM_015294873.1 Latest version!	Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa43087	XM_015294875.1 Latest version!	Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS1 ( XM_015294875.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015150361.2 Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa144742
Clone ID Related Accession (Same CDS sequence)	XM_015294875.2
Accession Version	XM_015294875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nitric oxide synthase, brain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG 
ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GATACGATGG CTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015150361.2
CDS	700..5007
Translation	MEENAFSVQQLQPNVISVRLFKRKVGGLGFLVKERVSKPPVIVSDLIRGGAAEQSGLVQAGDIILAVNGRPLVDTSYESALEILRSIASETYVVLILRGPEGFTTHLETTFAGDGTPKTIRVTRPLCPAPRAVELSNPSLHSQELPAAMGSLWTKETGRDVEPVVHVNGLVTGPKGQDSNKGKDSACSHLCLNGGVEDNELLKEIEPVLDLLKSSSKDGGGDVPCKVETRDIEVQVDCFREGENKLQKAFPAQMENDHVFGDLWGKSNVPVVLNNPYSEAEQPAPSGRQSPTKSTANGSPSKCPRFVKIKNWETGSVLHDTLHLKTAMTTACTEQICMGSVMTPSQHVRKSEETRTKEEVLLLAKDFIDQYYSSIKRSGSKAHMERLEEVTKEIEATDTYQLRDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDSKHDFRIWNAQLIRYAGYKQPDGTILGDPANVELTEICIQQGWKAPYGRFDILPLLLQANGNDPELFEIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEQVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSQVYAKTLCEIFKHAFDAKVMSMDEYDIVHLEHETMVLVVTSTFGNGDPPENGEKFGCALMEMKNPNSNLEERKSYKVRFNSVSSYSDARKSSSDGPDSRDNFESTGPLANVRFSVFGLGSRAYPHFCAFARAVDTLLEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRSKFRLTYVAEAPELTQGLYSIHKKRVYAARLITRQNLQSPKSSRSTIFLRLHTNGHQELQYLPGDHLGVFPGNHEELVNALIDRLEDAPPANQLVKVELLEERSTALGVISNWTDENRIPPCTIFQAFKYYLDITTPPTPLLLQQFALLATSDKEKKRLQVLSKGLQEYEEWKWSKNPTVVEVLEEFPSVQMPSTLLLTQLPLLQPRYYSISSSPEMYPGEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQSDEVVPCFVRGAPGFHLPQDPQVPCILIGPGTGIAPFRSFWQQRLFEIQHKGLKPCPMVLVFGCRQSRIDHIYKEETLFAKTQGVFRELYTAYSREPDKPKKYVQDVLQEQLAQTVYKALKEQRGHIYVCGDVTMAGDVLKTVQRIVRQQGQLSVEEAGTFISKLRDDSRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDDTMAL

Target ORF information:

RefSeq Version	XM_015294875.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294875.2

ORF Insert Sequence:

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG 
ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GATACGATGG CTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_015294873.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015150359.2 Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa15889
Clone ID Related Accession (Same CDS sequence)	XM_015294873.2 , XM_015294874.2 , XM_004934480.3
Accession Version	XM_015294873.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG 
ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015150359.2
CDS	554..4861
Translation	MEENAFSVQQLQPNVISVRLFKRKVGGLGFLVKERVSKPPVIVSDLIRGGAAEQSGLVQAGDIILAVNGRPLVDTSYESALEILRSIASETYVVLILRGPEGFTTHLETTFAGDGTPKTIRVTRPLCPAPRAVELSNPSLHSQELPAAMGSLWTKETGRDVEPVVHVNGLVTGPKGQDSNKGKDSACSHLCLNGGVEDNELLKEIEPVLDLLKSSSKDGGGDVPCKVETRDIEVQVDCFREGENKLQKAFPAQMENDHVFGDLWGKSNVPVVLNNPYSEAEQPAPSGRQSPTKSTANGSPSKCPRFVKIKNWETGSVLHDTLHLKTAMTTACTEQICMGSVMTPSQHVRKSEETRTKEEVLLLAKDFIDQYYSSIKRSGSKAHMERLEEVTKEIEATDTYQLRDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDSKHDFRIWNAQLIRYAGYKQPDGTILGDPANVELTEICIQQGWKAPYGRFDILPLLLQANGNDPELFEIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEQVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSQVYAKTLCEIFKHAFDAKVMSMDEYDIVHLEHETMVLVVTSTFGNGDPPENGEKFGCALMEMKNPNSNLEERKSYKVRFNSVSSYSDARKSSSDGPDSRDNFESTGPLANVRFSVFGLGSRAYPHFCAFARAVDTLLEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRSKFRLTYVAEAPELTQGLYSIHKKRVYAARLITRQNLQSPKSSRSTIFLRLHTNGHQELQYLPGDHLGVFPGNHEELVNALIDRLEDAPPANQLVKVELLEERSTALGVISNWTDENRIPPCTIFQAFKYYLDITTPPTPLLLQQFALLATSDKEKKRLQVLSKGLQEYEEWKWSKNPTVVEVLEEFPSVQMPSTLLLTQLPLLQPRYYSISSSPEMYPGEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQSDEVVPCFVRGAPGFHLPQDPQVPCILIGPGTGIAPFRSFWQQRLFEIQHKGLKPCPMVLVFGCRQSRIDHIYKEETLFAKTQGVFRELYTAYSREPDKPKKYVQDVLQEQLAQTVYKALKEQRGHIYVCGDVTMAGDVLKTVQRIVRQQGQLSVEEAGTFISKLRDDSRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFCS

Target ORF information:

RefSeq Version	XM_015294873.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294873.2

ORF Insert Sequence:

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG 
ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_015294874.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015150360.2 Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

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CloneID	OGa15889
Clone ID Related Accession (Same CDS sequence)	XM_015294873.2 , XM_015294874.2 , XM_004934480.3
Accession Version	XM_015294874.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015294874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG 
ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015150360.2
CDS	337..4644
Translation	MEENAFSVQQLQPNVISVRLFKRKVGGLGFLVKERVSKPPVIVSDLIRGGAAEQSGLVQAGDIILAVNGRPLVDTSYESALEILRSIASETYVVLILRGPEGFTTHLETTFAGDGTPKTIRVTRPLCPAPRAVELSNPSLHSQELPAAMGSLWTKETGRDVEPVVHVNGLVTGPKGQDSNKGKDSACSHLCLNGGVEDNELLKEIEPVLDLLKSSSKDGGGDVPCKVETRDIEVQVDCFREGENKLQKAFPAQMENDHVFGDLWGKSNVPVVLNNPYSEAEQPAPSGRQSPTKSTANGSPSKCPRFVKIKNWETGSVLHDTLHLKTAMTTACTEQICMGSVMTPSQHVRKSEETRTKEEVLLLAKDFIDQYYSSIKRSGSKAHMERLEEVTKEIEATDTYQLRDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDSKHDFRIWNAQLIRYAGYKQPDGTILGDPANVELTEICIQQGWKAPYGRFDILPLLLQANGNDPELFEIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEQVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSQVYAKTLCEIFKHAFDAKVMSMDEYDIVHLEHETMVLVVTSTFGNGDPPENGEKFGCALMEMKNPNSNLEERKSYKVRFNSVSSYSDARKSSSDGPDSRDNFESTGPLANVRFSVFGLGSRAYPHFCAFARAVDTLLEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRSKFRLTYVAEAPELTQGLYSIHKKRVYAARLITRQNLQSPKSSRSTIFLRLHTNGHQELQYLPGDHLGVFPGNHEELVNALIDRLEDAPPANQLVKVELLEERSTALGVISNWTDENRIPPCTIFQAFKYYLDITTPPTPLLLQQFALLATSDKEKKRLQVLSKGLQEYEEWKWSKNPTVVEVLEEFPSVQMPSTLLLTQLPLLQPRYYSISSSPEMYPGEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQSDEVVPCFVRGAPGFHLPQDPQVPCILIGPGTGIAPFRSFWQQRLFEIQHKGLKPCPMVLVFGCRQSRIDHIYKEETLFAKTQGVFRELYTAYSREPDKPKKYVQDVLQEQLAQTVYKALKEQRGHIYVCGDVTMAGDVLKTVQRIVRQQGQLSVEEAGTFISKLRDDSRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFCS

Target ORF information:

RefSeq Version	XM_015294874.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294874.2

ORF Insert Sequence:

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG 
ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_004934480.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004934537.3 Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

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CloneID	OGa15889
Clone ID Related Accession (Same CDS sequence)	XM_015294873.2 , XM_015294874.2 , XM_004934480.3
Accession Version	XM_004934480.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004934480.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG 
ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004934537.3
CDS	699..5006
Translation	MEENAFSVQQLQPNVISVRLFKRKVGGLGFLVKERVSKPPVIVSDLIRGGAAEQSGLVQAGDIILAVNGRPLVDTSYESALEILRSIASETYVVLILRGPEGFTTHLETTFAGDGTPKTIRVTRPLCPAPRAVELSNPSLHSQELPAAMGSLWTKETGRDVEPVVHVNGLVTGPKGQDSNKGKDSACSHLCLNGGVEDNELLKEIEPVLDLLKSSSKDGGGDVPCKVETRDIEVQVDCFREGENKLQKAFPAQMENDHVFGDLWGKSNVPVVLNNPYSEAEQPAPSGRQSPTKSTANGSPSKCPRFVKIKNWETGSVLHDTLHLKTAMTTACTEQICMGSVMTPSQHVRKSEETRTKEEVLLLAKDFIDQYYSSIKRSGSKAHMERLEEVTKEIEATDTYQLRDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDSKHDFRIWNAQLIRYAGYKQPDGTILGDPANVELTEICIQQGWKAPYGRFDILPLLLQANGNDPELFEIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEQVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSQVYAKTLCEIFKHAFDAKVMSMDEYDIVHLEHETMVLVVTSTFGNGDPPENGEKFGCALMEMKNPNSNLEERKSYKVRFNSVSSYSDARKSSSDGPDSRDNFESTGPLANVRFSVFGLGSRAYPHFCAFARAVDTLLEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRSKFRLTYVAEAPELTQGLYSIHKKRVYAARLITRQNLQSPKSSRSTIFLRLHTNGHQELQYLPGDHLGVFPGNHEELVNALIDRLEDAPPANQLVKVELLEERSTALGVISNWTDENRIPPCTIFQAFKYYLDITTPPTPLLLQQFALLATSDKEKKRLQVLSKGLQEYEEWKWSKNPTVVEVLEEFPSVQMPSTLLLTQLPLLQPRYYSISSSPEMYPGEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQSDEVVPCFVRGAPGFHLPQDPQVPCILIGPGTGIAPFRSFWQQRLFEIQHKGLKPCPMVLVFGCRQSRIDHIYKEETLFAKTQGVFRELYTAYSREPDKPKKYVQDVLQEQLAQTVYKALKEQRGHIYVCGDVTMAGDVLKTVQRIVRQQGQLSVEEAGTFISKLRDDSRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFCS

Target ORF information:

RefSeq Version	XM_004934480.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004934480.3

ORF Insert Sequence:

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGTCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCT GGCAGCATCA CCCCGGTTTT CCACCAGGAG 
ATGCTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_015294873.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015150359.1 Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa43086
Clone ID Related Accession (Same CDS sequence)	XM_015294873.1 , XM_015294874.1 , XM_004934480.2
Accession Version	XM_015294873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG 
ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015150359.1
CDS	560..4867
Translation	MEENAFSVQQLQPNVISVRLFKRKVGGLGFLVKERVSKPPVIVSDLIRGGAAEQSGLVQAGDIILAVNGRPLVDTSYESALEILRSIASETYVVLILRGPEGFTTHLETTFAGDGTPKTIRVTRPLCPAPRAVELSNPSLHSQELPAAMGSLWTKETGRDVEPVVHVNGLVTGPKGQDSNKGKDSACSHLCLNGGVEDNELLKEIEPVLDLLKSSSKDGGGDVPCKVETRDIEVQVDCFREGENKLQKAFPAQMENDHVFGDLWGKSNVPVVLNNPYSEAEQPAPSGRQSPTKSTANGSPSKCPRFVKIKNWETGSVLHDTLHLKTAMTTACTEQICMGSVMTPSQHVRKSEETRTKEEVLLLAKDFIDQYYSSIKRSGSKAHMERLEEVTKEIEATDTYQLRDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDSKHDFRIWNAQLIRYAGYKQPDGTILGDPANVELTEICIQQGWKAPYGRFDILPLLLQANGNDPELFEIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEQVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMFNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSQVYAKTLCEIFKHAFDAKVMSMDEYDIVHLEHETMVLVVTSTFGNGDPPENGEKFGCALMEMKNPNSNLEERKSYKVRFNSVSSYSDARKSSSDGPDSRDNFESTGPLANVRFSVFGLGSRAYPHFCAFARAVDTLLEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRSKFRLTYVAEAPELTQGLYSIHKKRVYAARLITRQNLQSPKSSRSTIFLRLHTNGHQELQYLPGDHLGVFPGNHEELVNALIDRLEDAPPANQLVKVELLEERSTALGVISNWTDENRIPPCTIFQAFKYYLDITTPPTPLLLQQFALLATSDKEKKRLQVLSKGLQEYEEWKWSKNPTVVEVLEEFPSVQMPSTLLLTQLPLLQPRYYSISSSPEMYPGEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQSDEVVPCFVRGAPGFHLPQDPQVPCILIGPGTGIAPFRSFWQQRLFEIQHKGLKPCPMVLVFGCRQSRIDHIYKEETLFAKTQGVFRELYTAYSREPDKPKKYVQDVLQEQLAQTVYKALKEQRGHIYVCGDVTMAGDVLKTVQRIVRQQGQLSVEEAGTFISKLRDDSRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFCS

Target ORF information:

RefSeq Version	XM_015294873.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294873.1

ORF Insert Sequence:

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG 
ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_015294874.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015150360.1 Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa43086
Clone ID Related Accession (Same CDS sequence)	XM_015294873.1 , XM_015294874.1 , XM_004934480.2
Accession Version	XM_015294874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG 
ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015150360.1
CDS	338..4645
Translation	MEENAFSVQQLQPNVISVRLFKRKVGGLGFLVKERVSKPPVIVSDLIRGGAAEQSGLVQAGDIILAVNGRPLVDTSYESALEILRSIASETYVVLILRGPEGFTTHLETTFAGDGTPKTIRVTRPLCPAPRAVELSNPSLHSQELPAAMGSLWTKETGRDVEPVVHVNGLVTGPKGQDSNKGKDSACSHLCLNGGVEDNELLKEIEPVLDLLKSSSKDGGGDVPCKVETRDIEVQVDCFREGENKLQKAFPAQMENDHVFGDLWGKSNVPVVLNNPYSEAEQPAPSGRQSPTKSTANGSPSKCPRFVKIKNWETGSVLHDTLHLKTAMTTACTEQICMGSVMTPSQHVRKSEETRTKEEVLLLAKDFIDQYYSSIKRSGSKAHMERLEEVTKEIEATDTYQLRDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDSKHDFRIWNAQLIRYAGYKQPDGTILGDPANVELTEICIQQGWKAPYGRFDILPLLLQANGNDPELFEIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEQVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMFNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSQVYAKTLCEIFKHAFDAKVMSMDEYDIVHLEHETMVLVVTSTFGNGDPPENGEKFGCALMEMKNPNSNLEERKSYKVRFNSVSSYSDARKSSSDGPDSRDNFESTGPLANVRFSVFGLGSRAYPHFCAFARAVDTLLEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRSKFRLTYVAEAPELTQGLYSIHKKRVYAARLITRQNLQSPKSSRSTIFLRLHTNGHQELQYLPGDHLGVFPGNHEELVNALIDRLEDAPPANQLVKVELLEERSTALGVISNWTDENRIPPCTIFQAFKYYLDITTPPTPLLLQQFALLATSDKEKKRLQVLSKGLQEYEEWKWSKNPTVVEVLEEFPSVQMPSTLLLTQLPLLQPRYYSISSSPEMYPGEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQSDEVVPCFVRGAPGFHLPQDPQVPCILIGPGTGIAPFRSFWQQRLFEIQHKGLKPCPMVLVFGCRQSRIDHIYKEETLFAKTQGVFRELYTAYSREPDKPKKYVQDVLQEQLAQTVYKALKEQRGHIYVCGDVTMAGDVLKTVQRIVRQQGQLSVEEAGTFISKLRDDSRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFCS

Target ORF information:

RefSeq Version	XM_015294874.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294874.1

ORF Insert Sequence:

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG 
ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_004934480.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004934537.2 Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA.

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CloneID	OGa43086
Clone ID Related Accession (Same CDS sequence)	XM_015294873.1 , XM_015294874.1 , XM_004934480.2
Accession Version	XM_004934480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004934480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG 
ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004934537.2
CDS	686..4993
Translation	MEENAFSVQQLQPNVISVRLFKRKVGGLGFLVKERVSKPPVIVSDLIRGGAAEQSGLVQAGDIILAVNGRPLVDTSYESALEILRSIASETYVVLILRGPEGFTTHLETTFAGDGTPKTIRVTRPLCPAPRAVELSNPSLHSQELPAAMGSLWTKETGRDVEPVVHVNGLVTGPKGQDSNKGKDSACSHLCLNGGVEDNELLKEIEPVLDLLKSSSKDGGGDVPCKVETRDIEVQVDCFREGENKLQKAFPAQMENDHVFGDLWGKSNVPVVLNNPYSEAEQPAPSGRQSPTKSTANGSPSKCPRFVKIKNWETGSVLHDTLHLKTAMTTACTEQICMGSVMTPSQHVRKSEETRTKEEVLLLAKDFIDQYYSSIKRSGSKAHMERLEEVTKEIEATDTYQLRDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDSKHDFRIWNAQLIRYAGYKQPDGTILGDPANVELTEICIQQGWKAPYGRFDILPLLLQANGNDPELFEIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEQVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMFNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSQVYAKTLCEIFKHAFDAKVMSMDEYDIVHLEHETMVLVVTSTFGNGDPPENGEKFGCALMEMKNPNSNLEERKSYKVRFNSVSSYSDARKSSSDGPDSRDNFESTGPLANVRFSVFGLGSRAYPHFCAFARAVDTLLEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRSKFRLTYVAEAPELTQGLYSIHKKRVYAARLITRQNLQSPKSSRSTIFLRLHTNGHQELQYLPGDHLGVFPGNHEELVNALIDRLEDAPPANQLVKVELLEERSTALGVISNWTDENRIPPCTIFQAFKYYLDITTPPTPLLLQQFALLATSDKEKKRLQVLSKGLQEYEEWKWSKNPTVVEVLEEFPSVQMPSTLLLTQLPLLQPRYYSISSSPEMYPGEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQSDEVVPCFVRGAPGFHLPQDPQVPCILIGPGTGIAPFRSFWQQRLFEIQHKGLKPCPMVLVFGCRQSRIDHIYKEETLFAKTQGVFRELYTAYSREPDKPKKYVQDVLQEQLAQTVYKALKEQRGHIYVCGDVTMAGDVLKTVQRIVRQQGQLSVEEAGTFISKLRDDSRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFCS

Target ORF information:

RefSeq Version	XM_004934480.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004934480.2

ORF Insert Sequence:

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG 
ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GAGGTTTTCT GCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_015294875.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015150361.1 Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X4, mRNA.

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CloneID	OGa43087
Clone ID Related Accession (Same CDS sequence)	XM_015294875.1
Accession Version	XM_015294875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	nitric oxide synthase, brain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455972.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG 
ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GATACGATGG CTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015150361.1
CDS	687..4994
Translation	MEENAFSVQQLQPNVISVRLFKRKVGGLGFLVKERVSKPPVIVSDLIRGGAAEQSGLVQAGDIILAVNGRPLVDTSYESALEILRSIASETYVVLILRGPEGFTTHLETTFAGDGTPKTIRVTRPLCPAPRAVELSNPSLHSQELPAAMGSLWTKETGRDVEPVVHVNGLVTGPKGQDSNKGKDSACSHLCLNGGVEDNELLKEIEPVLDLLKSSSKDGGGDVPCKVETRDIEVQVDCFREGENKLQKAFPAQMENDHVFGDLWGKSNVPVVLNNPYSEAEQPAPSGRQSPTKSTANGSPSKCPRFVKIKNWETGSVLHDTLHLKTAMTTACTEQICMGSVMTPSQHVRKSEETRTKEEVLLLAKDFIDQYYSSIKRSGSKAHMERLEEVTKEIEATDTYQLRDTELIYGAKHAWRNAARCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDSKHDFRIWNAQLIRYAGYKQPDGTILGDPANVELTEICIQQGWKAPYGRFDILPLLLQANGNDPELFEIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEQVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMFNYRLTPSFEYQPDPWNTHIWKGVNGTPTKKRAIGFKKLAKAVKFSAKLMGQAMAKRVKATILYATETGKSQVYAKTLCEIFKHAFDAKVMSMDEYDIVHLEHETMVLVVTSTFGNGDPPENGEKFGCALMEMKNPNSNLEERKSYKVRFNSVSSYSDARKSSSDGPDSRDNFESTGPLANVRFSVFGLGSRAYPHFCAFARAVDTLLEELGGERILRMGEGDELCGQEESFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRSKFRLTYVAEAPELTQGLYSIHKKRVYAARLITRQNLQSPKSSRSTIFLRLHTNGHQELQYLPGDHLGVFPGNHEELVNALIDRLEDAPPANQLVKVELLEERSTALGVISNWTDENRIPPCTIFQAFKYYLDITTPPTPLLLQQFALLATSDKEKKRLQVLSKGLQEYEEWKWSKNPTVVEVLEEFPSVQMPSTLLLTQLPLLQPRYYSISSSPEMYPGEVHLTVAVVSYRTRDGEGPIHHGVCSSWLNRIQSDEVVPCFVRGAPGFHLPQDPQVPCILIGPGTGIAPFRSFWQQRLFEIQHKGLKPCPMVLVFGCRQSRIDHIYKEETLFAKTQGVFRELYTAYSREPDKPKKYVQDVLQEQLAQTVYKALKEQRGHIYVCGDVTMAGDVLKTVQRIVRQQGQLSVEEAGTFISKLRDDSRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDDTMAL

Target ORF information:

RefSeq Version	XM_015294875.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015294875.1

ORF Insert Sequence:

ATGGAGGAGA ACGCCTTCAG TGTGCAGCAG CTGCAGCCCA ACGTCATCTC GGTGAGGCTG 
TTCAAGCGCA AGGTGGGGGG CCTGGGCTTC CTGGTGAAGG AGCGCGTCAG CAAGCCGCCC 
GTCATCGTCT CCGACCTGAT CCGGGGAGGG GCTGCTGAGC AGAGCGGCCT CGTGCAGGCT 
GGGGACATCA TTTTAGCTGT CAATGGGCGG CCCTTGGTGG ACACCAGCTA CGAGAGCGCG 
CTGGAGATCC TGAGGAGCAT CGCCTCCGAG ACCTACGTGG TGCTCATCCT GCGTGGCCCT 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTTGCAGGCG ACGGAACACC CAAAACCATC 
CGCGTCACCC GGCCCCTCTG CCCTGCACCC AGAGCTGTTG AGTTGTCCAA CCCCAGCCTT 
CACAGCCAGG AGTTGCCGGC AGCCATGGGT TCTCTCTGGA CAAAGGAGAC GGGGCGGGAC 
GTGGAGCCTG TGGTCCATGT CAACGGGTTG GTCACCGGCC CCAAAGGGCA GGATAGCAAC 
AAGGGGAAGG ACAGTGCCTG CAGCCATCTC TGCCTCAACG GTGGAGTGGA AGACAACGAG 
CTTCTCAAGG AGATTGAGCC TGTCCTGGAC CTCCTGAAGA GCAGCAGTAA GGATGGTGGT 
GGAGATGTGC CCTGCAAGGT AGAGACAAGG GATATAGAAG TCCAGGTGGA CTGTTTCAGG 
GAAGGGGAGA ACAAGCTGCA GAAAGCCTTC CCAGCTCAGA TGGAGAACGA CCACGTCTTT 
GGTGACCTGT GGGGGAAGAG CAATGTGCCC GTGGTCCTCA ACAACCCCTA CTCTGAAGCA 
GAGCAGCCGG CACCATCAGG GCGGCAGTCC CCGACAAAGA GCACAGCGAA TGGCAGTCCT 
TCCAAATGCC CGCGGTTTGT CAAAATCAAG AACTGGGAGA CGGGCTCTGT GCTCCATGAC 
ACTCTGCATC TCAAGACAGC AATGACCACC GCATGCACTG AGCAGATCTG CATGGGCTCT 
GTGATGACCC CGAGCCAGCA CGTCCGCAAG TCAGAAGAAA CCAGGACAAA AGAGGAAGTG 
CTCCTCCTGG CTAAGGACTT CATTGACCAG TACTACTCCT CCATTAAGAG ATCTGGCTCC 
AAGGCGCACA TGGAGAGGCT GGAGGAGGTG ACCAAGGAGA TTGAAGCTAC TGACACCTAC 
CAGCTGCGGG ACACAGAGCT GATCTATGGT GCAAAGCATG CCTGGAGGAA CGCAGCCCGC 
TGTGTGGGCA GGATTCAGTG GTCCAAGCTG CAGGTGTTTG ATGCTCGAGA CTGCACCACA 
GCCCATGGGA TGTTCAATTA CATCTGCAAT CACATCAAAT ACGCCACCAA CAAAGGGAAC 
CTCAGATCTG CCATCACCAT CTTCCCACAA CGGACAGACA GCAAGCACGA CTTCCGCATC 
TGGAACGCAC AGCTCATCCG CTACGCTGGC TACAAGCAGC CTGATGGCAC CATCCTGGGG 
GACCCTGCCA ACGTGGAGCT GACCGAGATC TGCATACAGC AAGGGTGGAA GGCACCGTAC 
GGCCGTTTCG ATATTCTTCC TCTGCTCCTC CAAGCCAACG GCAATGACCC AGAGCTCTTT 
GAAATCCCCC CAGAGCTTGT GCTGGAAGTG CCCATCCGGC ACCCCAAGTT CGAGTGGTTT 
AAGGACCTGG GCCTGAAGTG GTACGGTCTG CCTGCAGTGT CCAACATGCT CCTTGAGATT 
GGTGGCTTAG AGTTCTCCGC CTGCCCCTTC AGCGGCTGGT ACATGGGCAC AGAGATCGGG 
GTCCGGGATT ACTGCGACAA CTCCCGCTAC AACATCCTGG AGCAAGTGGC CAAGAAAATG 
AACCTGGACA TGAGGAAAAC CTCCTCGCTG TGGAAGGATC AGGCCCTGGT GGAGATCAAC 
ATCGCTGTGC TGTACAGCTT CCAGAGTGAC AAAGTGACTA TTGTGGACCA CCACTCGGCC 
ACCGAGTCCT TCATCAAGCA CATGGAGAAC GAGTACCGCT GCCGTGGGGG CTGCCCTGCT 
GACTGGGTGT GGATCGTGCC GCCCATGTCG GGCAGCATCA CCCCGGTGTT CCACCAGGAG 
ATGTTCAACT ACCGCCTCAC ACCCTCCTTT GAGTACCAGC CTGACCCCTG GAACACCCAC 
ATCTGGAAGG GAGTCAATGG GACGCCCACC AAGAAGAGGG CCATTGGCTT TAAGAAGTTG 
GCAAAAGCTG TGAAGTTCTC AGCCAAGCTG ATGGGACAAG CCATGGCCAA GAGGGTCAAA 
GCAACCATCC TGTATGCCAC GGAGACGGGG AAGTCACAGG TCTATGCAAA AACCCTGTGT 
GAAATCTTCA AGCATGCATT CGATGCCAAG GTGATGTCCA TGGATGAGTA TGACATTGTG 
CACCTAGAGC ATGAAACCAT GGTCCTGGTG GTCACAAGCA CCTTTGGCAA TGGAGACCCA 
CCTGAAAATG GAGAGAAATT TGGCTGTGCG CTCATGGAGA TGAAGAATCC CAACTCCAAC 
CTGGAGGAGA GGAAGAGCTA CAAGGTCCGT TTCAACAGTG TCTCCTCCTA CTCGGATGCA 
CGGAAGTCCT CAAGTGATGG CCCTGACTCC AGGGACAACT TTGAAAGTAC AGGGCCCCTA 
GCTAATGTCA GGTTCTCTGT GTTTGGGCTG GGTTCACGGG CATACCCCCA CTTCTGCGCC 
TTTGCACGAG CAGTGGACAC CTTGCTGGAG GAGCTGGGTG GTGAGCGGAT TCTGCGCATG 
GGAGAGGGGG ACGAGCTCTG CGGCCAGGAG GAGTCCTTCC GCACCTGGGC CAAGAAGGTC 
TTCAAGGCAG CGTGTGATGT CTTCTGCGTG GGAGATGATG TCAACATCGA GAAGGCCAAC 
AACTCCCTCA TCAGCAATGA CCGGAGCTGG AAGAGGAGCA AGTTCCGCCT CACCTACGTG 
GCCGAGGCCC CGGAGCTCAC ACAAGGGCTG TACAGCATCC ACAAAAAGCG TGTCTATGCA 
GCCCGGCTGA TCACACGCCA AAACCTGCAG AGCCCTAAGT CCAGTCGCTC GACCATTTTC 
CTGCGCCTCC ACACCAATGG CCACCAGGAG CTGCAGTACC TGCCTGGGGA CCACTTGGGT 
GTCTTTCCAG GGAACCACGA GGAGCTGGTC AATGCCCTGA TTGACAGGCT TGAGGATGCT 
CCTCCAGCCA ACCAGCTAGT AAAGGTGGAG CTCCTGGAGG AGAGGAGCAC AGCTCTAGGC 
GTCATCAGTA ACTGGACAGA TGAGAACCGC ATCCCACCAT GCACCATCTT CCAGGCTTTT 
AAATACTATC TGGACATCAC CACCCCTCCC ACCCCGCTGC TGCTACAGCA GTTTGCCTTG 
TTGGCCACCA GTGACAAGGA GAAGAAACGT CTGCAGGTCC TTAGCAAGGG CTTGCAGGAG 
TACGAGGAGT GGAAGTGGTC CAAGAACCCC ACCGTGGTGG AAGTGCTGGA GGAGTTCCCC 
TCGGTACAGA TGCCCTCCAC ACTGCTGCTC ACGCAGCTGC CCCTGCTGCA GCCCCGCTAC 
TACTCCATCA GCTCCTCTCC AGAGATGTAC CCTGGAGAGG TCCATCTCAC TGTGGCTGTG 
GTCTCCTACC GCACTAGAGA TGGAGAAGGC CCTATCCATC ATGGAGTCTG CTCCTCCTGG 
CTCAATAGGA TCCAGAGTGA CGAGGTCGTG CCCTGCTTTG TAAGAGGGGC TCCCGGGTTC 
CACCTGCCGC AGGACCCCCA GGTGCCCTGC ATCCTCATTG GCCCCGGCAC CGGCATTGCC 
CCATTCCGGA GCTTCTGGCA ACAGCGACTC TTTGAAATCC AGCACAAAGG CCTGAAGCCC 
TGTCCCATGG TTCTGGTGTT TGGGTGCCGG CAATCCAGGA TTGATCACAT CTACAAAGAG 
GAGACGCTTT TTGCCAAAAC CCAAGGTGTC TTCAGAGAGC TGTACACAGC CTACTCCCGG 
GAACCGGACA AACCAAAGAA ATACGTGCAG GACGTGTTAC AGGAGCAGCT GGCCCAAACG 
GTGTACAAAG CGCTGAAGGA GCAGAGAGGC CACATCTACG TCTGTGGGGA TGTCACCATG 
GCTGGGGATG TCCTCAAGAC CGTTCAGCGC ATCGTGAGGC AGCAGGGCCA GCTGTCGGTG 
GAGGAGGCAG GCACTTTCAT CAGCAAGCTA CGGGATGACA GCCGGTACCA CGAGGATATT 
TTTGGAGTCA CCCTGCGCAC GTACGAAGTG ACTAACCGCC TTAGATCCGA GTCTATTGCA 
TTCATTGAGG AGAGCAAAAA AGATACGGAT GATACGATGG CTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NOS1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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