Nos1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nos1
Entrez Gene ID	18125
Full Name	nitric oxide synthase 1, neuronal
Synonyms	2310005C01Rik,N-NOS,NC-NOS,NO,NOS,NOS-I,Nos-1,bNOS,nNOS
General protein information	Preferred Names nitric oxide synthase 1, neuronal Names nitric oxide synthase, brain NOS type I constitutive NOS neuronal NOS neuronal nitric oxide synthase peptidyl-cysteine S-nitrosylase NOS1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 F\|5 57.29 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017320707.1	XP_017176196.1	nitric oxide synthase, brain isoform X4
XM_006530189.3	XP_006530252.1	nitric oxide synthase, brain isoform X1
NM_008712.3	NP_032738.1	nitric oxide synthase, brain
XM_006530188.3	XP_006530251.1	nitric oxide synthase, brain isoform X1
XM_006530192.3	XP_006530255.1	nitric oxide synthase, brain isoform X3
XM_006530187.3	XP_006530250.1	nitric oxide synthase, brain isoform X1
XM_006530190.1	XP_006530253.1	nitric oxide synthase, brain isoform X1
XM_006530191.3	XP_006530254.1	nitric oxide synthase, brain isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NOS1	XP_003314004.2
Rattus norvegicus (Norway rat)	Nos1	NP_434686.1
Gallus gallus (chicken)	NOS1	XP_004934537.1
Xenopus tropicalis (tropical clawed frog)	nos1	XP_004910558.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008255	XP_317213.1
Macaca mulatta (Rhesus monkey)	NOS1	XP_001083352.2
Mus musculus (house mouse)	Nos1	NP_032738.1
Homo sapiens (human)	NOS1	NP_000611.1
Bos taurus (cattle)	NOS1	XP_002694631.3
Canis lupus familiaris (dog)	NOS1	NP_001182074.1
Danio rerio (zebrafish)	nos1	NP_571735.1
Drosophila melanogaster (fruit fly)	Nos	NP_523541.2

Gene Ontology
Bibliography

Related articles in PubMed

Temporally and Spatially Distinct Thirst Satiation Signals.
Augustine V, Ebisu H, Zhao Y, Lee S, Ho B, Mizuno GO, Tian L, Oka Y
Neuron103(2)242-249.e4(2019 07)

Macula Densa SGLT1-NOS1-Tubuloglomerular Feedback Pathway, a New Mechanism for Glomerular Hyperfiltration during Hyperglycemia.
Zhang J, Wei J, Jiang S, Xu L, Wang L, Cheng F, Buggs J, Koepsell H, Vallon V, Liu R
Journal of the American Society of Nephrology : JASN30(4)578-593(2019 04)

A gut-to-brain signal of fluid osmolarity controls thirst satiation.
Zimmerman CA, Huey EL, Ahn JS, Beutler LR, Tan CL, Kosar S, Bai L, Chen Y, Corpuz TV, Madisen L, Zeng H, Knight ZA
Nature568(7750)98-102(2019 04)

Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea.
Vyas P, Wu JS, Jimenez A, Glowatzki E, Fuchs PA
Scientific reports9(1)5549(2019 Apr)

Role of nitric oxide synthase on brain GABA transaminase activity and GABA levels.
Vega Rasgado LA, Reyes GC, Vega Díaz F
Acta pharmaceutica (Zagreb, Croatia)68(3)349-359(2018 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nos1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nos1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20278	NM_008712.3 Latest version!	Mus musculus nitric oxide synthase 1, neuronal (Nos1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu20278	XM_017320707.1 Latest version!	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu35811	XM_006530187.3 Latest version!	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu35811	XM_006530188.3 Latest version!	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu35811	XM_006530190.1 Latest version!	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu35811	XM_006530189.3 Latest version!	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu35812	XM_006530191.3 Latest version!	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu35813	XM_006530192.3 Latest version!	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nos1 ( NM_008712.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032738.1 Mus musculus nitric oxide synthase 1, neuronal (Nos1), mRNA.

Price & Availability↑

CloneID	OMu20278
Clone ID Related Accession (Same CDS sequence)	XM_017320707.1 , NM_008712.3
Accession Version	NM_008712.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577462400000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, brain
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC114617.16 and AC114993.18. On May 5, 2015 this sequence version replaced NM_008712.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC 
TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT 
GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA 
GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC 
ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC 
TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT 
GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT 
GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG 
ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC 
CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC 
GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT 
GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG 
GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG 
GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA 
GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG 
GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC 
ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT 
CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA 
GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT 
GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC 
CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG 
CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC 
TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG 
AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG 
AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG 
GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA 
GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA 
ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA 
AAAGACACAG ATGAGGTTTT CAGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032738.1
CDS	379..4668
Translation	MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	NM_008712.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 1, neuronal (Nos1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008712.3

ORF Insert Sequence:

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC 
TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT 
GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA 
GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC 
ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC 
TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT 
GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT 
GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG 
ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC 
CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC 
GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT 
GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG 
GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG 
GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA 
GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG 
GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC 
ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT 
CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA 
GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT 
GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC 
CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG 
CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC 
TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG 
AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG 
AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG 
GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA 
GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA 
ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA 
AAAGACACAG ATGAGGTTTT CAGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos1 ( XM_017320707.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176196.1 Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu20278
Clone ID Related Accession (Same CDS sequence)	XM_017320707.1 , NM_008712.3
Accession Version	XM_017320707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, brain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC 
TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT 
GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA 
GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC 
ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC 
TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT 
GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT 
GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG 
ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC 
CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC 
GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT 
GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG 
GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG 
GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA 
GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG 
GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC 
ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT 
CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA 
GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT 
GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC 
CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG 
CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC 
TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG 
AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG 
AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG 
GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA 
GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA 
ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA 
AAAGACACAG ATGAGGTTTT CAGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176196.1
CDS	1109..5398
Translation	MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_017320707.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320707.1

ORF Insert Sequence:

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC 
TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT 
GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA 
GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC 
ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC 
TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT 
GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT 
GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG 
ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC 
CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC 
GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT 
GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG 
GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG 
GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA 
GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG 
GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC 
ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT 
CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA 
GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT 
GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC 
CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG 
CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC 
TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG 
AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG 
AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG 
GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA 
GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA 
ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA 
AAAGACACAG ATGAGGTTTT CAGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos1 ( XM_006530189.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530252.1 Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X3, mRNA.

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CloneID	OMu35811
Clone ID Related Accession (Same CDS sequence)	XM_006530189.3 , XM_006530188.3 , XM_006530187.3 , XM_006530190.1
Accession Version	XM_006530189.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530189.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT 
CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG 
GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT 
TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT 
GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG 
AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT 
AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG 
GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG 
GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG 
CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT 
TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG 
GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530252.1
CDS	508..4902
Translation	MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPSLSHADSEAHSLVAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDDRVFSS

Target ORF information:

RefSeq Version	XM_006530189.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530189.3

ORF Insert Sequence:

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT 
CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG 
GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT 
TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT 
GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG 
AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT 
AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG 
GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG 
GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG 
CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT 
TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG 
GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos1 ( XM_006530188.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530251.1 Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X2, mRNA.

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CloneID	OMu35811
Clone ID Related Accession (Same CDS sequence)	XM_006530189.3 , XM_006530188.3 , XM_006530187.3 , XM_006530190.1
Accession Version	XM_006530188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530188.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT 
CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG 
GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT 
TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT 
GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG 
AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT 
AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG 
GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG 
GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG 
CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT 
TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG 
GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530251.1
CDS	541..4935
Translation	MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPSLSHADSEAHSLVAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDDRVFSS

Target ORF information:

RefSeq Version	XM_006530188.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530188.3

ORF Insert Sequence:

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT 
CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG 
GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT 
TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT 
GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG 
AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT 
AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG 
GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG 
GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG 
CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT 
TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG 
GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos1 ( XM_006530187.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530250.1 Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35811
Clone ID Related Accession (Same CDS sequence)	XM_006530189.3 , XM_006530188.3 , XM_006530187.3 , XM_006530190.1
Accession Version	XM_006530187.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530187.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT 
CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG 
GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT 
TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT 
GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG 
AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT 
AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG 
GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG 
GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG 
CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT 
TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG 
GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530250.1
CDS	1113..5507
Translation	MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPSLSHADSEAHSLVAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDDRVFSS

Target ORF information:

RefSeq Version	XM_006530187.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530187.3

ORF Insert Sequence:

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT 
CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG 
GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT 
TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT 
GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG 
AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT 
AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG 
GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG 
GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG 
CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT 
TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG 
GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos1 ( XM_006530190.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530253.1 Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu35811
Clone ID Related Accession (Same CDS sequence)	XM_006530189.3 , XM_006530188.3 , XM_006530187.3 , XM_006530190.1
Accession Version	XM_006530190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT 
CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG 
GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT 
TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT 
GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG 
AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT 
AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG 
GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG 
GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG 
CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT 
TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG 
GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530253.1
CDS	529..4923
Translation	MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPSLSHADSEAHSLVAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDDRVFSS

Target ORF information:

RefSeq Version	XM_006530190.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530190.1

ORF Insert Sequence:

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT 
CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG 
GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT 
TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT 
GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG 
AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT 
AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG 
GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG 
GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG 
CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT 
TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGACAGG 
GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos1 ( XM_006530191.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530254.1 Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X5, mRNA.

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CloneID	OMu35812
Clone ID Related Accession (Same CDS sequence)	XM_006530191.3
Accession Version	XM_006530191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, brain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT 
CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG 
GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT 
TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT 
GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG 
AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT 
AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG 
GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG 
GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG 
CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT 
TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGAGGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530254.1
CDS	1112..5503
Translation	MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPSLSHADSEAHSLVAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_006530191.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530191.3

ORF Insert Sequence:

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGC TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAGCACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGCAAG TCATCAGGCG ATGGACCAGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC GCTGGCCAAT GTGAGGTTCT CAGTCTTCGG CCTGGGCTCT 
CGGGCATACC CTCACTTCTG TGCCTTTGGG CACGCAGTGG ACACTCTCCT GGAGGAGCTG 
GGCGGGGAGA GGATTCTGAA GATGAGGGAG GGAGATGAGC TCTGCGGACA GGAAGAGGCT 
TTCAGGACCT GGGCTAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG TGTGGGGGAT 
GACGTCAACA TTGAGAAGGC GAACAATTCC CTCATTAGCA ATGACCGCAG CTGGAAGAGG 
AATAAATTCC GCCTCACTTA TGTGGCAGAA GCTCCAGAGC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AGCGAGTCTC AGCCGCCCGA CTCCTCAGCC GCCAAAACCT GCAAAGTCCT 
AAATCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGTTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCTGGCAACC ATGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA CGCGCCGCCT GCCAACCACG TGGTGAAGGT GGAAATGCTG 
GAGGAAAGGA ACACGGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCCCTGGCC ACCAATGAGA AAGAGAAGCA GCGGCTGCTG 
GTCCTCAGCA AGGGGCTTCA GGAATATGAG GAATGGAAAT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CCACACTTCT CCTCACACAG 
CTGTCGCTGC TACAACCTCG CTACTATTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTGTCC TACCATACCC GAGATGGAGA AGGACCGGTT 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC CGAATACAGG CTGACGATGT CGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACCGGCAT CGCTCCCTTC CGAAGTTTTT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTACTC CCGGGAACCC GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCCGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCATATT 
TATGTCTGCG GGGACGTTAC CATGGCCGCT GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC GGAGGAGGAT GCTGGTGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGG GTCACCCTCA GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATT GAAGAGAGCA AAAAAGACAC AGATGAGGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos1 ( XM_006530192.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530255.1 Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X6, mRNA.

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CloneID	OMu35813
Clone ID Related Accession (Same CDS sequence)	XM_006530192.3
Accession Version	XM_006530192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	nitric oxide synthase, brain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC 
TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT 
GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA 
GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC 
ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC 
TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT 
GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT 
GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG 
ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC 
CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC 
GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT 
GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG 
GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG 
GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA 
GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG 
GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC 
ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT 
CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA 
GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT 
GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC 
CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG 
CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC 
TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG 
AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG 
AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG 
GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA 
GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA 
ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA 
AAAGACACAG ATGACAGGGT TTTCAGCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530255.1
CDS	1110..5402
Translation	MEEHTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGTPTKAVDLSRQPSASKDQPLAVDRVPGPSNGPQHAQGRGQGAGSVSQANGVAIDPTMKNTKANLQDSGEQDELLKEIEPVLSILTGGGKAVNRGGPAKAEMKDTGIQVDRDLDGKLHKAPPLGGENDRVFNDLWGKGNVPVVLNNPYSENEQSPASGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEQICMGSIMLPSHHIRKSEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVEFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDDRVFSS

Target ORF information:

RefSeq Version	XM_006530192.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nitric oxide synthase 1, neuronal (Nos1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530192.3

ORF Insert Sequence:

ATGGAAGAGC ACACGTTTGG GGTCCAGCAG ATCCAACCCA ACGTCATTTC TGTCCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCCCCG 
GTGATCATCT CAGATCTGAT CCGAGGAGGT GCCGCGGAGC AGAGCGGCCT TATCCAAGCC 
GGCGACATCA TTCTCGCCGT CAATGATCGG CCCCTGGTAG ACCTCAGCTA TGACAGCGCT 
CTGGAAGTTC TCAGGGGCAT TGCCTCTGAG ACCCATGTGG TCCTCATTCT GAGAGGCCCT 
GAGGGCTTTA CTACACACCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGTGTGACCC AGCCCCTGGG GACACCCACC AAAGCTGTCG ATCTGTCTCG CCAGCCATCA 
GCCAGCAAAG ACCAGCCATT AGCAGTAGAC AGGGTCCCAG GTCCCAGTAA CGGACCTCAG 
CATGCCCAAG GCCGTGGACA GGGAGCCGGC TCAGTCTCCC AGGCTAATGG TGTGGCCATT 
GACCCCACAA TGAAAAATAC CAAGGCCAAC CTCCAGGACA GCGGAGAACA GGATGAACTG 
CTCAAAGAGA TAGAACCTGT GTTGAGCATC CTCACCGGTG GGGGAAAAGC AGTCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAC 
GGCAAACTGC ACAAAGCTCC GCCCCTGGGC GGGGAGAATG ATCGAGTCTT CAATGATCTG 
TGGGGGAAGG GCAACGTCCC TGTGGTCCTC AACAACCCGT ATTCAGAGAA CGAACAGTCT 
CCCGCCTCGG GCAAACAGTC TCCTACCAAG AATGGCAGTC CTTCCAGGTG CCCTCGCTTC 
CTTAAGGTCA AGAACTGGGA GACAGATGTG GTCCTCACTG ACACCCTGCA CCTGAAGAGC 
ACGCTGGAAA CGGGGTGCAC AGAGCAGATC TGCATGGGCT CCATCATGCT GCCATCCCAT 
CACATACGGA AGTCAGAAGA TGTCCGCACC AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC CTCCATTAAG AGGTTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATCGAGAGC ACCAGCACCT ACCAGCTCAA GGACACGGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GCTGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCATGG GATGTTCAAC 
TACATCTGTA ACCACGTCAA GTACGCCACC AACAAAGGGA ACCTCAGGTC GGCCATCACT 
ATATTCCCTC AAAGGACTGA TGGCAAGCAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCAGATGGC TCTACCTTGG GCGATCCAGC TAATGTGGAG 
TTCACAGAGA TCTGTATACA GCAGGGCTGG AAACCCCCAA GAGGCCGGTT CGATGTGCTG 
CCTCTCCTGC TCCAGGCCAA TGGCAATGAC CCTGAACTCT TTCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TACCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TTAGTGGCTG GTACATGGGC ACGGAGATTG GCGTTCGTGA TTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAAA TGGATTTGGA CATGAGGAAG 
ACATCCTCCC TCTGGAAGGA CCAAGCCCTG GTGGAGATTA ACATTGCTGT CCTATACAGC 
TTCCAGAGTG ACAAGGTGAC CATTGTGGAC CACCACTCTG CCACCGAGTC CTTCATCAAA 
CACATGGAGA ATGAGTACCG CTGCAGAGGG GGCTGTCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGGCTC 
ACCCCGTCCT TTGAGTACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAT 
GGGACCCCCA CGAAGCGGCG AGCCATTGGC TTCAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TGATGGGACA GGCCATGGCC AAGAGAGTCA AAGCGACCAT TCTCTACGCC 
ACCGAGACAG GCAAATCCCA AGCCTATGCC AAGACCCTGT GCGAGATCTT CAAGCATGCC 
TTCGATGCCA AGGCTATGTC CATGGAGGAA TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCAGAGAA TGGGGAGAAA 
TTCGGCTGTG CTTTGATGGA GATGAGGCAC CCCAACTCTG TGCAAGAGGA GAGGAAGAGC 
TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGCAAGTC ATCAGGCGAT 
GGACCAGACC TCAGAGACAA CTTTGAAAGT ACTGGACCGC TGGCCAATGT GAGGTTCTCA 
GTCTTCGGCC TGGGCTCTCG GGCATACCCT CACTTCTGTG CCTTTGGGCA CGCAGTGGAC 
ACTCTCCTGG AGGAGCTGGG CGGGGAGAGG ATTCTGAAGA TGAGGGAGGG AGATGAGCTC 
TGCGGACAGG AAGAGGCTTT CAGGACCTGG GCTAAGAAAG TCTTCAAGGC AGCCTGTGAT 
GTGTTCTGTG TGGGGGATGA CGTCAACATT GAGAAGGCGA ACAATTCCCT CATTAGCAAT 
GACCGCAGCT GGAAGAGGAA TAAATTCCGC CTCACTTATG TGGCAGAAGC TCCAGAGCTG 
ACCCAAGGTC TTTCCAATGT TCACAAAAAG CGAGTCTCAG CCGCCCGACT CCTCAGCCGC 
CAAAACCTGC AAAGTCCTAA ATCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC 
GGGAATCAGG AGTTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC TGGCAACCAT 
GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGACG CGCCGCCTGC CAACCACGTG 
GTGAAGGTGG AAATGCTGGA GGAAAGGAAC ACGGCTCTGG GTGTCATCAG TAATTGGAAG 
GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CCCTGGCCAC CAATGAGAAA 
GAGAAGCAGC GGCTGCTGGT CCTCAGCAAG GGGCTTCAGG AATATGAGGA ATGGAAATGG 
GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCC 
ACACTTCTCC TCACACAGCT GTCGCTGCTA CAACCTCGCT ACTATTCCAT CAGCTCCTCT 
CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTGTCCTA CCATACCCGA 
GATGGAGAAG GACCGGTTCA CCACGGGGTG TGCTCCTCCT GGCTCAACCG AATACAGGCT 
GACGATGTCG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC 
CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACCGGCATCG CTCCCTTCCG AAGTTTTTGG 
CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC 
TTCGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG 
AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTACTCCC GGGAACCCGA CAGGCCAAAG 
AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCCGAGT CTGTGTACCG CGCCCTGAAG 
GAGCAAGGAG GCCATATTTA TGTCTGCGGG GACGTTACCA TGGCCGCTGA TGTCCTCAAA 
GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCGG AGGAGGATGC TGGTGTGTTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGGGT CACCCTCAGA 
ACATATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATTGA AGAGAGCAAA 
AAAGACACAG ATGACAGGGT TTTCAGCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Temporally and Spatially Distinct Thirst Satiation Signals.
Neuron103(2)242-249.e4(2019 07)
Augustine V,Ebisu H,Zhao Y,Lee S,Ho B,Mizuno GO,Tian L,Oka Y

Macula Densa SGLT1-NOS1-Tubuloglomerular Feedback Pathway, a New Mechanism for Glomerular Hyperfiltration during Hyperglycemia.
Journal of the American Society of Nephrology : JASN30(4)578-593(2019 04)
Zhang J,Wei J,Jiang S,Xu L,Wang L,Cheng F,Buggs J,Koepsell H,Vallon V,Liu R

A gut-to-brain signal of fluid osmolarity controls thirst satiation.
Nature568(7750)98-102(2019 04)
Zimmerman CA,Huey EL,Ahn JS,Beutler LR,Tan CL,Kosar S,Bai L,Chen Y,Corpuz TV,Madisen L,Zeng H,Knight ZA

Characterization of transgenic mouse lines for labeling type I and type II afferent neurons in the cochlea.
Scientific reports9(1)5549(2019 Apr)
Vyas P,Wu JS,Jimenez A,Glowatzki E,Fuchs PA

Role of nitric oxide synthase on brain GABA transaminase activity and GABA levels.
Acta pharmaceutica (Zagreb, Croatia)68(3)349-359(2018 Sep)
Vega Rasgado LA,Reyes GC,Vega Díaz F

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Nos1 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

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Target ORF information:

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