Nos1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nos1
Entrez Gene ID	24598
Full Name	nitric oxide synthase 1
Synonyms	bNOS
General protein information	Preferred Names nitric oxide synthase 1 Names nitric oxide synthase, brain N-NOS NC-NOS NOS type I constitutive NOS nNOS neuronal NOS nitric oxidase synthase nitric oxide synthase 1, neuronal peptidyl-cysteine S-nitrosylase NOS1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16
Summary	enzyme that catalyzes the production of nitric oxide [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598256.1	XP_017453745.1	nitric oxide synthase, brain isoform X1
NM_052799.1	NP_434686.1	nitric oxide synthase, brain
XM_017598257.1	XP_017453746.1	nitric oxide synthase, brain isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NOS1	XP_003314004.2
Rattus norvegicus (Norway rat)	Nos1	NP_434686.1
Gallus gallus (chicken)	NOS1	XP_004934537.1
Xenopus tropicalis (tropical clawed frog)	nos1	XP_004910558.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008255	XP_317213.1
Macaca mulatta (Rhesus monkey)	NOS1	XP_001083352.2
Mus musculus (house mouse)	Nos1	NP_032738.1
Homo sapiens (human)	NOS1	NP_000611.1
Bos taurus (cattle)	NOS1	XP_002694631.3
Canis lupus familiaris (dog)	NOS1	NP_001182074.1
Danio rerio (zebrafish)	nos1	NP_571735.1
Drosophila melanogaster (fruit fly)	Nos	NP_523541.2

Gene Ontology
Bibliography

Related articles in PubMed

Mechanism and regulation of ferrous heme-nitric oxide (NO) oxidation in NO synthases.
Tejero J, Hunt AP, Santolini J, Lehnert N, Stuehr DJ
The Journal of biological chemistry294(19)7904-7916(2019 05)

Effect of TRPV1 on Activity of Isoforms of Constitutive Nitric Oxide Synthase during Regulation of Bicarbonate Secretion in the Stomach.
Zolotarev VA, Andreeva YV, Khropycheva RP
Bulletin of experimental biology and medicine166(3)310-312(2019 Jan)

The essential role of hypothalamic paraventricular nucleus nNOS in the modulation of autonomic control in exercised rats.
Raquel HA, Ferreira NZ, Lucchetti BFC, Falquetto B, Pinge-Filho P, Michelini LC, Martins-Pinge MC
Nitric oxide : biology and chemistry7914-24(2018 09)

PSD95 and nNOS interaction as a novel molecular target to modulate conditioned fear: relevance to PTSD.
Li LP, Dustrude ET, Haulcomb MM, Abreu AR, Fitz SD, Johnson PL, Thakur GA, Molosh AI, Lai Y, Shekhar A
Translational psychiatry8(1)155(2018 08)

Nitric oxide in skeletal muscle.
Kobzik L, Reid MB, Bredt DS, Stamler JS
Nature372(6506)546-8(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nos1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nos1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13258	NM_052799.1 Latest version!	Rattus norvegicus nitric oxide synthase 1 (Nos1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa42930	XM_017598256.1 Latest version!	Rattus norvegicus nitric oxide synthase 1 (Nos1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa57779	XM_017598257.1 Latest version!	Rattus norvegicus nitric oxide synthase 1 (Nos1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nos1 ( NM_052799.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_434686.1 Rattus norvegicus nitric oxide synthase 1 (Nos1), mRNA.

Price & Availability↑

CloneID	ORa13258
Clone ID Related Accession (Same CDS sequence)	NM_052799.1
Accession Version	NM_052799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Rattus norvegicus(Norway rat)
Product	nitric oxide synthase, brain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U67309.1. On Dec 17, 2003 this sequence version replaced XM_346438.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U67309.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAGA ACACGTTTGG GGTTCAGCAG ATCCAACCCA ATGTAATTTC TGTTCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCTCCC 
GTGATCATCT CAGACCTGAT TCGAGGAGGT GCTGCGGAGC AGAGCGGCCT TATCCAAGCT 
GGAGACATCA TTCTCGCAGT CAACGATCGG CCCTTGGTAG ACCTCAGCTA TGACAGTGCC 
CTGGAGGTTC TCAGGGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAGGGCTTCA CTACACATCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGGGTGACCC AGCCCCTCGG TCCTCCCACC AAAGCCGTCG ATCTGTCTCA CCAGCCTTCA 
GCCAGCAAAG ACCAGTCATT AGCAGTAGAC AGAGTCACAG GTCTGGGTAA TGGCCCTCAG 
CATGCCCAAG GCCATGGGCA GGGAGCTGGC TCAGTCTCCC AAGCTAATGG TGTGGCCATT 
GACCCCACGA TGAAAAGCAC CAAGGCCAAC CTCCAGGACA TCGGGGAACA TGATGAACTG 
CTCAAAGAGA TAGAACCTGT GCTGAGCATC CTCAACAGTG GGAGCAAAGC CACCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAT 
GGCAAATCGC ACAAAGCTCC GCCCCTGGGC GGGGACAATG ACCGCGTCTT CAATGACCTG 
TGGGGGAAGG ACAACGTTCC TGTGGTCCTT AACAACCCGT ATTCAGAGAA GGAACAGTCC 
CCTACCTCGG GGAAACAGTC TCCCACCAAG AACGGCAGCC CTTCCAGGTG CCCCCGTTTC 
CTCAAGGTCA AGAACTGGGA GACGGACGTG GTCCTCACCG ACACCCTGCA CCTGAAGAGC 
ACACTGGAAA CGGGGTGCAC AGAGCACATT TGCATGGGCT CGATCATGCT GCCTTCCCAG 
CACACGCGGA AGCCAGAAGA TGTCCGCACA AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC ATCCATTAAG AGATTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATTGAAAGC ACCAGCACCT ACCAGCTCAA GGACACCGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GATGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCACGG CATGTTCAAC 
TACATCTGTA ACCATGTCAA GTATGCCACC AACAAAGGGA ATCTCAGGTC GGCCATCACG 
ATATTCCCTC AGAGGACTGA CGGCAAACAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTACGCGG GCTACAAGCA GCCAGATGGC TCTACCTTGG GGGATCCAGC CAATGTGCAG 
TTCACGGAGA TCTGTATACA GCAGGGCTGG AAAGCCCCAA GAGGCCGCTT CGACGTGCTG 
CCTCTCCTGC TTCAGGCCAA TGGCAATGAC CCTGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TGCCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TCGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATCG GCGTCCGTGA CTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAGA TGGATTTGGA CATGAGGAAG 
ACCTCGTCCC TCTGGAAGGA CCAAGCACTG GTGGAGATCA ACATTGCTGT TCTATATAGC 
TTCCAGAGTG ACAAGGTGAC CATCGTTGAC CACCACTCTG CCACGGAGTC CTTCATCAAA 
CACATGGAGA ATGAATACCG CTGCAGAGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCTCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTATAGACTC 
ACCCCGTCCT TTGAATACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAC 
GGGACCCCCA CGAAGCGGCG AGCTATCGGC TTTAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TAATGGGGCA GGCCATGGCC AAGAGGGTCA AGGCGACCAT TCTCTACGCC 
ACAGAGACAG GCAAATCACA AGCCTATGCC AAGACCCTGT GTGAGATCTT CAAGCACGCC 
TTCGATGCCA AGGCAATGTC CATGGAGGAG TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCTGAGAA CGGGGAGAAA 
TTCGGCTGTG CTTTAATGGA GATGAGGCAC CCCAACTCTG TGCAGGAGGA GAGAAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGT TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAACACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGAAAG TCATCGGGCG ACGGACCCGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC CCTGGCCAAT GTGAGGTTCT CAGTGTTCGG CCTCGGCTCT 
CGGGCGTACC CCCACTTCTG TGCCTTTGGG CATGCGGTGG ACACCCTCCT GGAGGAACTG 
GGAGGGGAGA GGATTCTGAA GATGAGGGAG GGGGATGAGC TTTGCGGACA GGAAGAAGCT 
TTCAGGACCT GGGCCAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG CGTGGGGGAT 
GACGTCAACA TCGAGAAGGC GAACAACTCC CTCATTAGCA ATGACCGAAG CTGGAAGAGG 
AACAAGTTCC GCCTCACGTA TGTGGCGGAA GCTCCAGATC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AACGAGTCTC GGCTGCTCGA CTCCTCAGCC GCCAAAACCT GCAAAGCCCT 
AAGTCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGCTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCCGGCAACC ACGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA TGCACCGCCT GCCAACCACG TGGTGAAGGT GGAGATGCTG 
GAGGAGAGGA ACACTGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCTCTGGCC ACTAATGAGA AAGAGAAGCA GCGGTTGCTG 
GTCCTCAGCA AGGGGCTCCA GGAATATGAG GAGTGGAAGT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CTACACTTCT CCTCACTCAG 
CTGTCGCTGC TGCAGCCTCG CTACTACTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACTGTGGC CATCGTCTCC TACCACACCC GAGACGGAGA AGGACCAGTC 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC AGAATACAGG CTGACGATGT AGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACTGGCAT CGCACCCTTC CGAAGCTTCT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGTCTATTC CCGGGAACCG GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCTGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCACATT 
TATGTCTGTG GGGACGTTAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC AGAGGAGGAC GCTGGTGTAT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGA GTCACCCTCA GAACGTATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATC GAAGAGAGCA AAAAAGACGC AGATGAGGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_434686.1
CDS	102..4493
Translation	MEENTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPSASKDQSLAVDRVTGLGNGPQHAQGHGQGAGSVSQANGVAIDPTMKSTKANLQDIGEHDELLKEIEPVLSILNSGSKATNRGGPAKAEMKDTGIQVDRDLDGKSHKAPPLGGDNDRVFNDLWGKDNVPVVLNNPYSEKEQSPTSGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPSQHTRKPEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKAPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPSLSHVDSEAHSLVAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPDLTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTVYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDADEVFSS

Target ORF information:

RefSeq Version	NM_052799.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nitric oxide synthase 1 (Nos1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052799.1

ORF Insert Sequence:

ATGGAAGAGA ACACGTTTGG GGTTCAGCAG ATCCAACCCA ATGTAATTTC TGTTCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCTCCC 
GTGATCATCT CAGACCTGAT TCGAGGAGGT GCTGCGGAGC AGAGCGGCCT TATCCAAGCT 
GGAGACATCA TTCTCGCAGT CAACGATCGG CCCTTGGTAG ACCTCAGCTA TGACAGTGCC 
CTGGAGGTTC TCAGGGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAGGGCTTCA CTACACATCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGGGTGACCC AGCCCCTCGG TCCTCCCACC AAAGCCGTCG ATCTGTCTCA CCAGCCTTCA 
GCCAGCAAAG ACCAGTCATT AGCAGTAGAC AGAGTCACAG GTCTGGGTAA TGGCCCTCAG 
CATGCCCAAG GCCATGGGCA GGGAGCTGGC TCAGTCTCCC AAGCTAATGG TGTGGCCATT 
GACCCCACGA TGAAAAGCAC CAAGGCCAAC CTCCAGGACA TCGGGGAACA TGATGAACTG 
CTCAAAGAGA TAGAACCTGT GCTGAGCATC CTCAACAGTG GGAGCAAAGC CACCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAT 
GGCAAATCGC ACAAAGCTCC GCCCCTGGGC GGGGACAATG ACCGCGTCTT CAATGACCTG 
TGGGGGAAGG ACAACGTTCC TGTGGTCCTT AACAACCCGT ATTCAGAGAA GGAACAGTCC 
CCTACCTCGG GGAAACAGTC TCCCACCAAG AACGGCAGCC CTTCCAGGTG CCCCCGTTTC 
CTCAAGGTCA AGAACTGGGA GACGGACGTG GTCCTCACCG ACACCCTGCA CCTGAAGAGC 
ACACTGGAAA CGGGGTGCAC AGAGCACATT TGCATGGGCT CGATCATGCT GCCTTCCCAG 
CACACGCGGA AGCCAGAAGA TGTCCGCACA AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC ATCCATTAAG AGATTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATTGAAAGC ACCAGCACCT ACCAGCTCAA GGACACCGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GATGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCACGG CATGTTCAAC 
TACATCTGTA ACCATGTCAA GTATGCCACC AACAAAGGGA ATCTCAGGTC GGCCATCACG 
ATATTCCCTC AGAGGACTGA CGGCAAACAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTACGCGG GCTACAAGCA GCCAGATGGC TCTACCTTGG GGGATCCAGC CAATGTGCAG 
TTCACGGAGA TCTGTATACA GCAGGGCTGG AAAGCCCCAA GAGGCCGCTT CGACGTGCTG 
CCTCTCCTGC TTCAGGCCAA TGGCAATGAC CCTGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TGCCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TCGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATCG GCGTCCGTGA CTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAGA TGGATTTGGA CATGAGGAAG 
ACCTCGTCCC TCTGGAAGGA CCAAGCACTG GTGGAGATCA ACATTGCTGT TCTATATAGC 
TTCCAGAGTG ACAAGGTGAC CATCGTTGAC CACCACTCTG CCACGGAGTC CTTCATCAAA 
CACATGGAGA ATGAATACCG CTGCAGAGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCTCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTATAGACTC 
ACCCCGTCCT TTGAATACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAC 
GGGACCCCCA CGAAGCGGCG AGCTATCGGC TTTAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TAATGGGGCA GGCCATGGCC AAGAGGGTCA AGGCGACCAT TCTCTACGCC 
ACAGAGACAG GCAAATCACA AGCCTATGCC AAGACCCTGT GTGAGATCTT CAAGCACGCC 
TTCGATGCCA AGGCAATGTC CATGGAGGAG TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCTGAGAA CGGGGAGAAA 
TTCGGCTGTG CTTTAATGGA GATGAGGCAC CCCAACTCTG TGCAGGAGGA GAGAAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGT TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAACACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGAAAG TCATCGGGCG ACGGACCCGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC CCTGGCCAAT GTGAGGTTCT CAGTGTTCGG CCTCGGCTCT 
CGGGCGTACC CCCACTTCTG TGCCTTTGGG CATGCGGTGG ACACCCTCCT GGAGGAACTG 
GGAGGGGAGA GGATTCTGAA GATGAGGGAG GGGGATGAGC TTTGCGGACA GGAAGAAGCT 
TTCAGGACCT GGGCCAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG CGTGGGGGAT 
GACGTCAACA TCGAGAAGGC GAACAACTCC CTCATTAGCA ATGACCGAAG CTGGAAGAGG 
AACAAGTTCC GCCTCACGTA TGTGGCGGAA GCTCCAGATC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AACGAGTCTC GGCTGCTCGA CTCCTCAGCC GCCAAAACCT GCAAAGCCCT 
AAGTCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGCTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCCGGCAACC ACGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA TGCACCGCCT GCCAACCACG TGGTGAAGGT GGAGATGCTG 
GAGGAGAGGA ACACTGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCTCTGGCC ACTAATGAGA AAGAGAAGCA GCGGTTGCTG 
GTCCTCAGCA AGGGGCTCCA GGAATATGAG GAGTGGAAGT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CTACACTTCT CCTCACTCAG 
CTGTCGCTGC TGCAGCCTCG CTACTACTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACTGTGGC CATCGTCTCC TACCACACCC GAGACGGAGA AGGACCAGTC 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC AGAATACAGG CTGACGATGT AGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACTGGCAT CGCACCCTTC CGAAGCTTCT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTCGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGTCTATTC CCGGGAACCG GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCTGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCACATT 
TATGTCTGTG GGGACGTTAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC AGAGGAGGAC GCTGGTGTAT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGA GTCACCCTCA GAACGTATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATC GAAGAGAGCA AAAAAGACGC AGATGAGGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos1 ( XM_017598256.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453745.1 Rattus norvegicus nitric oxide synthase 1 (Nos1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa42930
Clone ID Related Accession (Same CDS sequence)	XM_017598256.1
Accession Version	XM_017598256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA ACACGTTTGG GGTTCAGCAG ATCCAACCCA ATGTAATTTC TGTTCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCTCCC 
GTGATCATCT CAGACCTGAT TCGAGGAGGT GCTGCGGAGC AGAGCGGCCT TATCCAAGCT 
GGAGACATCA TTCTCGCAGT CAACGATCGG CCCTTGGTAG ACCTCAGCTA TGACAGTGCC 
CTGGAGGTTC TCAGGGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAGGGCTTCA CTACACATCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGGGTGACCC AGCCCCTCGG TCCTCCCACC AAAGCCGTCG ATCTGTCTCA CCAGCCTTCA 
GCCAGCAAAG ACCAGTCATT AGCAGTAGAC AGAGTCACAG GTCTGGGTAA TGGCCCTCAG 
CATGCCCAAG GCCATGGGCA GGGAGCTGGC TCAGTCTCCC AAGCTAATGG TGTGGCCATT 
GACCCCACGA TGAAAAGCAC CAAGGCCAAC CTCCAGGACA TCGGGGAACA TGATGAACTG 
CTCAAAGAGA TAGAACCTGT GCTGAGCATC CTCAACAGTG GGAGCAAAGC CACCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAT 
GGCAAATCGC ACAAAGCTCC GCCCCTGGGC GGGGACAATG ACCGCGTCTT CAATGACCTG 
TGGGGGAAGG ACAACGTTCC TGTGGTCCTT AACAACCCGT ATTCAGAGAA GGAACAGTCC 
CCTACCTCGG GGAAACAGTC TCCCACCAAG AACGGCAGCC CTTCCAGGTG CCCCCGTTTC 
CTCAAGGTCA AGAACTGGGA GACGGACGTG GTCCTCACCG ACACCCTGCA CCTGAAGAGC 
ACACTGGAAA CGGGGTGCAC AGAGCACATC TGCATGGGCT CGATCATGCT GCCTTCCCAG 
CACACGCGGA AGCCAGAAGA TGTCCGCACA AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC ATCCATTAAG AGATTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATTGAAAGC ACCAGCACCT ACCAGCTCAA GGACACCGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GATGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCACGG CATGTTCAAC 
TACATCTGTA ACCATGTCAA GTATGCCACC AACAAAGGGA ATCTCAGGTC GGCCATCACG 
ATATTCCCTC AGAGGACTGA CGGCAAACAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTACGCGG GCTACAAGCA GCCAGATGGC TCTACCTTGG GGGATCCAGC CAATGTGCAG 
TTCACGGAGA TCTGTATACA GCAGGGCTGG AAAGCCCCAA GAGGCCGCTT CGACGTGCTG 
CCTCTCCTGC TTCAGGCCAA TGGCAATGAC CCTGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TGCCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TCGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATCG GCGTCCGTGA CTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAGA TGGATTTGGA CATGAGGAAG 
ACCTCGTCCC TCTGGAAGGA CCAAGCACTG GTGGAGATCA ACATTGCTGT TCTATATAGC 
TTCCAGAGTG ACAAGGTGAC CATCGTTGAC CACCACTCTG CCACGGAGTC CTTCATCAAA 
CACATGGAGA ATGAATACCG CTGCAGAGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCTCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTATAGACTC 
ACCCCGTCCT TTGAATACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAC 
GGGACCCCCA CGAAGCGGCG AGCTATCGGC TTTAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TAATGGGGCA GGCCATGGCC AAGAGGGTCA AGGCGACCAT TCTCTACGCC 
ACAGAGACAG GCAAATCACA AGCCTATGCC AAGACCCTGT GTGAGATCTT CAAGCACGCC 
TTCGATGCCA AGGCAATGTC CATGGAGGAG TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCTGAGAA CGGGGAGAAA 
TTCGGCTGTG CTTTAATGGA GATGAGGCAC CCCAACTCTG TGCAGGAGGA GAGAAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGT TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAACACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGAAAG TCATCGGGCG ACGGACCCGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC CCTGGCCAAT GTGAGGTTCT CAGTGTTCGG CCTCGGCTCT 
CGGGCGTACC CCCACTTCTG TGCCTTTGGG CATGCGGTGG ACACCCTCCT GGAGGAACTG 
GGAGGGGAGA GGATTCTGAA GATGAGGGAG GGGGATGAGC TTTGCGGACA GGAAGAAGCT 
TTCAGGACCT GGGCCAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG CGTGGGGGAT 
GACGTCAACA TCGAGAAGGC GAACAACTCC CTCATTAGCA ATGACCGAAG CTGGAAGAGG 
AACAAGTTCC GCCTCACGTA TGTGGCGGAA GCTCCAGATC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AACGAGTCTC GGCTGCTCGA CTCCTCAGCC GCCAAAACCT GCAAAGCCCT 
AAGTCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGCTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCCGGCAACC ACGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA TGCACCGCCT GCCAACCACG TGGTGAAGGT GGAGATGCTG 
GAGGAGAGGA ACACTGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCTCTGGCC ACTAATGAGA AAGAGAAGCA GCGGTTGCTG 
GTCCTCAGCA AGGGGCTCCA GGAATATGAG GAGTGGAAGT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CTACACTTCT CCTCACTCAG 
CTGTCGCTGC TGCAGCCTCG CTACTACTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTCTCC TACCACACCC GAGACGGAGA AGGACCGGTC 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC AGAATACAGG CTGACGATGT AGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACTGGCAT CGCACCCTTC CGAAGCTTCT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTTGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTATTC CCGGGAACCG GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCTGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCACATT 
TATGTCTGTG GGGACGTTAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC AGAGGAGGAC GCTGGTGTAT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGA GTCACCCTCA GAACGTATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATC GAAGAGAGCA AAAAAGACGC AGATGAGGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453745.1
CDS	1175..5566
Translation	MEENTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPSASKDQSLAVDRVTGLGNGPQHAQGHGQGAGSVSQANGVAIDPTMKSTKANLQDIGEHDELLKEIEPVLSILNSGSKATNRGGPAKAEMKDTGIQVDRDLDGKSHKAPPLGGDNDRVFNDLWGKDNVPVVLNNPYSEKEQSPTSGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPSQHTRKPEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKAPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPSLSHVDSEAHSLVAARDSQHRSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPDLTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDADEVFSS

Target ORF information:

RefSeq Version	XM_017598256.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nitric oxide synthase 1 (Nos1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598256.1

ORF Insert Sequence:

ATGGAAGAGA ACACGTTTGG GGTTCAGCAG ATCCAACCCA ATGTAATTTC TGTTCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCTCCC 
GTGATCATCT CAGACCTGAT TCGAGGAGGT GCTGCGGAGC AGAGCGGCCT TATCCAAGCT 
GGAGACATCA TTCTCGCAGT CAACGATCGG CCCTTGGTAG ACCTCAGCTA TGACAGTGCC 
CTGGAGGTTC TCAGGGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAGGGCTTCA CTACACATCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGGGTGACCC AGCCCCTCGG TCCTCCCACC AAAGCCGTCG ATCTGTCTCA CCAGCCTTCA 
GCCAGCAAAG ACCAGTCATT AGCAGTAGAC AGAGTCACAG GTCTGGGTAA TGGCCCTCAG 
CATGCCCAAG GCCATGGGCA GGGAGCTGGC TCAGTCTCCC AAGCTAATGG TGTGGCCATT 
GACCCCACGA TGAAAAGCAC CAAGGCCAAC CTCCAGGACA TCGGGGAACA TGATGAACTG 
CTCAAAGAGA TAGAACCTGT GCTGAGCATC CTCAACAGTG GGAGCAAAGC CACCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAT 
GGCAAATCGC ACAAAGCTCC GCCCCTGGGC GGGGACAATG ACCGCGTCTT CAATGACCTG 
TGGGGGAAGG ACAACGTTCC TGTGGTCCTT AACAACCCGT ATTCAGAGAA GGAACAGTCC 
CCTACCTCGG GGAAACAGTC TCCCACCAAG AACGGCAGCC CTTCCAGGTG CCCCCGTTTC 
CTCAAGGTCA AGAACTGGGA GACGGACGTG GTCCTCACCG ACACCCTGCA CCTGAAGAGC 
ACACTGGAAA CGGGGTGCAC AGAGCACATC TGCATGGGCT CGATCATGCT GCCTTCCCAG 
CACACGCGGA AGCCAGAAGA TGTCCGCACA AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC ATCCATTAAG AGATTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATTGAAAGC ACCAGCACCT ACCAGCTCAA GGACACCGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GATGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCACGG CATGTTCAAC 
TACATCTGTA ACCATGTCAA GTATGCCACC AACAAAGGGA ATCTCAGGTC GGCCATCACG 
ATATTCCCTC AGAGGACTGA CGGCAAACAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTACGCGG GCTACAAGCA GCCAGATGGC TCTACCTTGG GGGATCCAGC CAATGTGCAG 
TTCACGGAGA TCTGTATACA GCAGGGCTGG AAAGCCCCAA GAGGCCGCTT CGACGTGCTG 
CCTCTCCTGC TTCAGGCCAA TGGCAATGAC CCTGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TGCCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TCGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATCG GCGTCCGTGA CTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAGA TGGATTTGGA CATGAGGAAG 
ACCTCGTCCC TCTGGAAGGA CCAAGCACTG GTGGAGATCA ACATTGCTGT TCTATATAGC 
TTCCAGAGTG ACAAGGTGAC CATCGTTGAC CACCACTCTG CCACGGAGTC CTTCATCAAA 
CACATGGAGA ATGAATACCG CTGCAGAGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCTCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTATAGACTC 
ACCCCGTCCT TTGAATACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAC 
GGGACCCCCA CGAAGCGGCG AGCTATCGGC TTTAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TAATGGGGCA GGCCATGGCC AAGAGGGTCA AGGCGACCAT TCTCTACGCC 
ACAGAGACAG GCAAATCACA AGCCTATGCC AAGACCCTGT GTGAGATCTT CAAGCACGCC 
TTCGATGCCA AGGCAATGTC CATGGAGGAG TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCTGAGAA CGGGGAGAAA 
TTCGGCTGTG CTTTAATGGA GATGAGGCAC CCCAACTCTG TGCAGGAGGA GAGAAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTTCCC TCTCCCATGT TGACTCTGAA 
GCCCACAGTC TGGTTGCTGC CCGTGACAGC CAACACAGGA GCTACAAGGT CCGATTCAAC 
AGCGTCTCCT CCTATTCTGA CTCCCGAAAG TCATCGGGCG ACGGACCCGA CCTCAGAGAC 
AACTTTGAAA GTACTGGACC CCTGGCCAAT GTGAGGTTCT CAGTGTTCGG CCTCGGCTCT 
CGGGCGTACC CCCACTTCTG TGCCTTTGGG CATGCGGTGG ACACCCTCCT GGAGGAACTG 
GGAGGGGAGA GGATTCTGAA GATGAGGGAG GGGGATGAGC TTTGCGGACA GGAAGAAGCT 
TTCAGGACCT GGGCCAAGAA AGTCTTCAAG GCAGCCTGTG ATGTGTTCTG CGTGGGGGAT 
GACGTCAACA TCGAGAAGGC GAACAACTCC CTCATTAGCA ATGACCGAAG CTGGAAGAGG 
AACAAGTTCC GCCTCACGTA TGTGGCGGAA GCTCCAGATC TGACCCAAGG TCTTTCCAAT 
GTTCACAAAA AACGAGTCTC GGCTGCTCGA CTCCTCAGCC GCCAAAACCT GCAAAGCCCT 
AAGTCCAGCC GATCGACCAT CTTCGTGCGT CTCCACACCA ACGGGAATCA GGAGCTGCAG 
TACCAGCCAG GGGACCACCT GGGTGTCTTC CCCGGCAACC ACGAGGACCT CGTGAATGCA 
CTCATTGAAC GGCTGGAGGA TGCACCGCCT GCCAACCACG TGGTGAAGGT GGAGATGCTG 
GAGGAGAGGA ACACTGCTCT GGGTGTCATC AGTAATTGGA AGGATGAATC TCGCCTCCCA 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACGCC GCCCACGCCC 
CTGCAGCTGC AGCAGTTCGC CTCTCTGGCC ACTAATGAGA AAGAGAAGCA GCGGTTGCTG 
GTCCTCAGCA AGGGGCTCCA GGAATATGAG GAGTGGAAGT GGGGCAAGAA CCCCACAATG 
GTGGAGGTGC TGGAGGAGTT CCCGTCCATC CAGATGCCGG CTACACTTCT CCTCACTCAG 
CTGTCGCTGC TGCAGCCTCG CTACTACTCC ATCAGCTCCT CTCCAGACAT GTACCCCGAC 
GAGGTGCACC TCACCGTGGC CATCGTCTCC TACCACACCC GAGACGGAGA AGGACCGGTC 
CACCACGGGG TGTGCTCCTC CTGGCTCAAC AGAATACAGG CTGACGATGT AGTCCCCTGC 
TTCGTGAGAG GTGCCCCTAG CTTCCACCTG CCTCGAAACC CCCAGGTGCC TTGCATCCTG 
GTTGGCCCAG GCACTGGCAT CGCACCCTTC CGAAGCTTCT GGCAACAGCG ACAATTTGAC 
ATCCAACACA AAGGAATGAA TCCGTGCCCC ATGGTTCTGG TCTTTGGGTG TCGACAATCC 
AAGATAGATC ATATCTACAG AGAGGAGACC CTGCAGGCCA AGAACAAGGG CGTCTTCAGA 
GAGCTGTACA CTGCCTATTC CCGGGAACCG GACAGGCCAA AGAAATATGT ACAGGACGTG 
CTGCAGGAAC AGCTGGCTGA GTCTGTGTAC CGCGCCCTGA AGGAGCAAGG AGGCCACATT 
TATGTCTGTG GGGACGTTAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATAATG 
ACCCAGCAGG GGAAACTCTC AGAGGAGGAC GCTGGTGTAT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCACGAGGA CATCTTTGGA GTCACCCTCA GAACGTATGA AGTGACCAAC 
CGCCTTAGAT CTGAGTCCAT CGCCTTCATC GAAGAGAGCA AAAAAGACGC AGATGAGGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nos1 ( XM_017598257.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453746.1 Rattus norvegicus nitric oxide synthase 1 (Nos1), transcript variant X2, mRNA.

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CloneID	ORa57779
Clone ID Related Accession (Same CDS sequence)	XM_017598257.1
Accession Version	XM_017598257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	nitric oxide synthase, brain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGA ACACGTTTGG GGTTCAGCAG ATCCAACCCA ATGTAATTTC TGTTCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCTCCC 
GTGATCATCT CAGACCTGAT TCGAGGAGGT GCTGCGGAGC AGAGCGGCCT TATCCAAGCT 
GGAGACATCA TTCTCGCAGT CAACGATCGG CCCTTGGTAG ACCTCAGCTA TGACAGTGCC 
CTGGAGGTTC TCAGGGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAGGGCTTCA CTACACATCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGGGTGACCC AGCCCCTCGG TCCTCCCACC AAAGCCGTCG ATCTGTCTCA CCAGCCTTCA 
GCCAGCAAAG ACCAGTCATT AGCAGTAGAC AGAGTCACAG GTCTGGGTAA TGGCCCTCAG 
CATGCCCAAG GCCATGGGCA GGGAGCTGGC TCAGTCTCCC AAGCTAATGG TGTGGCCATT 
GACCCCACGA TGAAAAGCAC CAAGGCCAAC CTCCAGGACA TCGGGGAACA TGATGAACTG 
CTCAAAGAGA TAGAACCTGT GCTGAGCATC CTCAACAGTG GGAGCAAAGC CACCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAT 
GGCAAATCGC ACAAAGCTCC GCCCCTGGGC GGGGACAATG ACCGCGTCTT CAATGACCTG 
TGGGGGAAGG ACAACGTTCC TGTGGTCCTT AACAACCCGT ATTCAGAGAA GGAACAGTCC 
CCTACCTCGG GGAAACAGTC TCCCACCAAG AACGGCAGCC CTTCCAGGTG CCCCCGTTTC 
CTCAAGGTCA AGAACTGGGA GACGGACGTG GTCCTCACCG ACACCCTGCA CCTGAAGAGC 
ACACTGGAAA CGGGGTGCAC AGAGCACATC TGCATGGGCT CGATCATGCT GCCTTCCCAG 
CACACGCGGA AGCCAGAAGA TGTCCGCACA AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC ATCCATTAAG AGATTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATTGAAAGC ACCAGCACCT ACCAGCTCAA GGACACCGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GATGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCACGG CATGTTCAAC 
TACATCTGTA ACCATGTCAA GTATGCCACC AACAAAGGGA ATCTCAGGTC GGCCATCACG 
ATATTCCCTC AGAGGACTGA CGGCAAACAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTACGCGG GCTACAAGCA GCCAGATGGC TCTACCTTGG GGGATCCAGC CAATGTGCAG 
TTCACGGAGA TCTGTATACA GCAGGGCTGG AAAGCCCCAA GAGGCCGCTT CGACGTGCTG 
CCTCTCCTGC TTCAGGCCAA TGGCAATGAC CCTGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TGCCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TCGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATCG GCGTCCGTGA CTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAGA TGGATTTGGA CATGAGGAAG 
ACCTCGTCCC TCTGGAAGGA CCAAGCACTG GTGGAGATCA ACATTGCTGT TCTATATAGC 
TTCCAGAGTG ACAAGGTGAC CATCGTTGAC CACCACTCTG CCACGGAGTC CTTCATCAAA 
CACATGGAGA ATGAATACCG CTGCAGAGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCTCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTATAGACTC 
ACCCCGTCCT TTGAATACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAC 
GGGACCCCCA CGAAGCGGCG AGCTATCGGC TTTAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TAATGGGGCA GGCCATGGCC AAGAGGGTCA AGGCGACCAT TCTCTACGCC 
ACAGAGACAG GCAAATCACA AGCCTATGCC AAGACCCTGT GTGAGATCTT CAAGCACGCC 
TTCGATGCCA AGGCAATGTC CATGGAGGAG TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCTGAGAA CGGGGAGAAA 
TTCGGCTGTG CTTTAATGGA GATGAGGCAC CCCAACTCTG TGCAGGAGGA GAGAAAGAGC 
TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGAAAGTC ATCGGGCGAC 
GGACCCGACC TCAGAGACAA CTTTGAAAGT ACTGGACCCC TGGCCAATGT GAGGTTCTCA 
GTGTTCGGCC TCGGCTCTCG GGCGTACCCC CACTTCTGTG CCTTTGGGCA TGCGGTGGAC 
ACCCTCCTGG AGGAACTGGG AGGGGAGAGG ATTCTGAAGA TGAGGGAGGG GGATGAGCTT 
TGCGGACAGG AAGAAGCTTT CAGGACCTGG GCCAAGAAAG TCTTCAAGGC AGCCTGTGAT 
GTGTTCTGCG TGGGGGATGA CGTCAACATC GAGAAGGCGA ACAACTCCCT CATTAGCAAT 
GACCGAAGCT GGAAGAGGAA CAAGTTCCGC CTCACGTATG TGGCGGAAGC TCCAGATCTG 
ACCCAAGGTC TTTCCAATGT TCACAAAAAA CGAGTCTCGG CTGCTCGACT CCTCAGCCGC 
CAAAACCTGC AAAGCCCTAA GTCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC 
GGGAATCAGG AGCTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC CGGCAACCAC 
GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGATG CACCGCCTGC CAACCACGTG 
GTGAAGGTGG AGATGCTGGA GGAGAGGAAC ACTGCTCTGG GTGTCATCAG TAATTGGAAG 
GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CTCTGGCCAC TAATGAGAAA 
GAGAAGCAGC GGTTGCTGGT CCTCAGCAAG GGGCTCCAGG AATATGAGGA GTGGAAGTGG 
GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCT 
ACACTTCTCC TCACTCAGCT GTCGCTGCTG CAGCCTCGCT ACTACTCCAT CAGCTCCTCT 
CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTCTCCTA CCACACCCGA 
GACGGAGAAG GACCGGTCCA CCACGGGGTG TGCTCCTCCT GGCTCAACAG AATACAGGCT 
GACGATGTAG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC 
CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACTGGCATCG CACCCTTCCG AAGCTTCTGG 
CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC 
TTTGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG 
AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTATTCCC GGGAACCGGA CAGGCCAAAG 
AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCTGAGT CTGTGTACCG CGCCCTGAAG 
GAGCAAGGAG GCCACATTTA TGTCTGTGGG GACGTTACCA TGGCCGCCGA TGTCCTCAAA 
GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCAG AGGAGGACGC TGGTGTATTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGAGT CACCCTCAGA 
ACGTATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATCGA AGAGAGCAAA 
AAAGACGCAG ATGAGGTTTT CAGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453746.1
CDS	904..5193
Translation	MEENTFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNDRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPSASKDQSLAVDRVTGLGNGPQHAQGHGQGAGSVSQANGVAIDPTMKSTKANLQDIGEHDELLKEIEPVLSILNSGSKATNRGGPAKAEMKDTGIQVDRDLDGKSHKAPPLGGDNDRVFNDLWGKDNVPVVLNNPYSEKEQSPTSGKQSPTKNGSPSRCPRFLKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPSQHTRKPEDVRTKDQLFPLAKEFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKAPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKAMSMEEYDIVHLEHEALVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSRKSSGDGPDLRDNFESTGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPDLTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDADEVFSS

Target ORF information:

RefSeq Version	XM_017598257.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nitric oxide synthase 1 (Nos1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598257.1

ORF Insert Sequence:

ATGGAAGAGA ACACGTTTGG GGTTCAGCAG ATCCAACCCA ATGTAATTTC TGTTCGTCTC 
TTCAAACGCA AAGTGGGAGG TCTGGGCTTC CTGGTGAAGG AACGGGTCAG CAAGCCTCCC 
GTGATCATCT CAGACCTGAT TCGAGGAGGT GCTGCGGAGC AGAGCGGCCT TATCCAAGCT 
GGAGACATCA TTCTCGCAGT CAACGATCGG CCCTTGGTAG ACCTCAGCTA TGACAGTGCC 
CTGGAGGTTC TCAGGGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT 
GAGGGCTTCA CTACACATCT GGAGACCACC TTCACAGGGG ATGGAACCCC CAAGACCATC 
CGGGTGACCC AGCCCCTCGG TCCTCCCACC AAAGCCGTCG ATCTGTCTCA CCAGCCTTCA 
GCCAGCAAAG ACCAGTCATT AGCAGTAGAC AGAGTCACAG GTCTGGGTAA TGGCCCTCAG 
CATGCCCAAG GCCATGGGCA GGGAGCTGGC TCAGTCTCCC AAGCTAATGG TGTGGCCATT 
GACCCCACGA TGAAAAGCAC CAAGGCCAAC CTCCAGGACA TCGGGGAACA TGATGAACTG 
CTCAAAGAGA TAGAACCTGT GCTGAGCATC CTCAACAGTG GGAGCAAAGC CACCAACAGA 
GGGGGACCAG CCAAAGCAGA GATGAAAGAC ACAGGAATCC AGGTGGACAG AGACCTCGAT 
GGCAAATCGC ACAAAGCTCC GCCCCTGGGC GGGGACAATG ACCGCGTCTT CAATGACCTG 
TGGGGGAAGG ACAACGTTCC TGTGGTCCTT AACAACCCGT ATTCAGAGAA GGAACAGTCC 
CCTACCTCGG GGAAACAGTC TCCCACCAAG AACGGCAGCC CTTCCAGGTG CCCCCGTTTC 
CTCAAGGTCA AGAACTGGGA GACGGACGTG GTCCTCACCG ACACCCTGCA CCTGAAGAGC 
ACACTGGAAA CGGGGTGCAC AGAGCACATC TGCATGGGCT CGATCATGCT GCCTTCCCAG 
CACACGCGGA AGCCAGAAGA TGTCCGCACA AAGGACCAGC TCTTCCCTCT AGCCAAAGAA 
TTTCTCGACC AATACTACTC ATCCATTAAG AGATTTGGCT CCAAGGCCCA CATGGACAGG 
CTGGAGGAGG TGAACAAGGA GATTGAAAGC ACCAGCACCT ACCAGCTCAA GGACACCGAG 
CTCATCTATG GCGCCAAGCA TGCCTGGCGG AACGCCTCTC GATGTGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGA GACTGCACCA CAGCCCACGG CATGTTCAAC 
TACATCTGTA ACCATGTCAA GTATGCCACC AACAAAGGGA ATCTCAGGTC GGCCATCACG 
ATATTCCCTC AGAGGACTGA CGGCAAACAT GACTTCCGAG TGTGGAACTC GCAGCTCATC 
CGCTACGCGG GCTACAAGCA GCCAGATGGC TCTACCTTGG GGGATCCAGC CAATGTGCAG 
TTCACGGAGA TCTGTATACA GCAGGGCTGG AAAGCCCCAA GAGGCCGCTT CGACGTGCTG 
CCTCTCCTGC TTCAGGCCAA TGGCAATGAC CCTGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGCTGGAAG TGCCCATCAG GCACCCCAAG TTCGACTGGT TTAAGGACCT GGGGCTCAAA 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TCGGGGGCCT GGAGTTCAGC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATCG GCGTCCGTGA CTACTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTA GCCAAGAAGA TGGATTTGGA CATGAGGAAG 
ACCTCGTCCC TCTGGAAGGA CCAAGCACTG GTGGAGATCA ACATTGCTGT TCTATATAGC 
TTCCAGAGTG ACAAGGTGAC CATCGTTGAC CACCACTCTG CCACGGAGTC CTTCATCAAA 
CACATGGAGA ATGAATACCG CTGCAGAGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCTCCCATGT CGGGCAGCAT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTATAGACTC 
ACCCCGTCCT TTGAATACCA GCCTGATCCA TGGAACACCC ACGTGTGGAA GGGCACCAAC 
GGGACCCCCA CGAAGCGGCG AGCTATCGGC TTTAAGAAAT TGGCAGAGGC CGTCAAGTTC 
TCAGCCAAGC TAATGGGGCA GGCCATGGCC AAGAGGGTCA AGGCGACCAT TCTCTACGCC 
ACAGAGACAG GCAAATCACA AGCCTATGCC AAGACCCTGT GTGAGATCTT CAAGCACGCC 
TTCGATGCCA AGGCAATGTC CATGGAGGAG TATGACATCG TGCACCTGGA GCACGAAGCC 
CTGGTCTTGG TGGTCACCAG CACCTTTGGC AATGGAGACC CCCCTGAGAA CGGGGAGAAA 
TTCGGCTGTG CTTTAATGGA GATGAGGCAC CCCAACTCTG TGCAGGAGGA GAGAAAGAGC 
TACAAGGTCC GATTCAACAG CGTCTCCTCC TATTCTGACT CCCGAAAGTC ATCGGGCGAC 
GGACCCGACC TCAGAGACAA CTTTGAAAGT ACTGGACCCC TGGCCAATGT GAGGTTCTCA 
GTGTTCGGCC TCGGCTCTCG GGCGTACCCC CACTTCTGTG CCTTTGGGCA TGCGGTGGAC 
ACCCTCCTGG AGGAACTGGG AGGGGAGAGG ATTCTGAAGA TGAGGGAGGG GGATGAGCTT 
TGCGGACAGG AAGAAGCTTT CAGGACCTGG GCCAAGAAAG TCTTCAAGGC AGCCTGTGAT 
GTGTTCTGCG TGGGGGATGA CGTCAACATC GAGAAGGCGA ACAACTCCCT CATTAGCAAT 
GACCGAAGCT GGAAGAGGAA CAAGTTCCGC CTCACGTATG TGGCGGAAGC TCCAGATCTG 
ACCCAAGGTC TTTCCAATGT TCACAAAAAA CGAGTCTCGG CTGCTCGACT CCTCAGCCGC 
CAAAACCTGC AAAGCCCTAA GTCCAGCCGA TCGACCATCT TCGTGCGTCT CCACACCAAC 
GGGAATCAGG AGCTGCAGTA CCAGCCAGGG GACCACCTGG GTGTCTTCCC CGGCAACCAC 
GAGGACCTCG TGAATGCACT CATTGAACGG CTGGAGGATG CACCGCCTGC CAACCACGTG 
GTGAAGGTGG AGATGCTGGA GGAGAGGAAC ACTGCTCTGG GTGTCATCAG TAATTGGAAG 
GATGAATCTC GCCTCCCACC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACGCCGC CCACGCCCCT GCAGCTGCAG CAGTTCGCCT CTCTGGCCAC TAATGAGAAA 
GAGAAGCAGC GGTTGCTGGT CCTCAGCAAG GGGCTCCAGG AATATGAGGA GTGGAAGTGG 
GGCAAGAACC CCACAATGGT GGAGGTGCTG GAGGAGTTCC CGTCCATCCA GATGCCGGCT 
ACACTTCTCC TCACTCAGCT GTCGCTGCTG CAGCCTCGCT ACTACTCCAT CAGCTCCTCT 
CCAGACATGT ACCCCGACGA GGTGCACCTC ACCGTGGCCA TCGTCTCCTA CCACACCCGA 
GACGGAGAAG GACCGGTCCA CCACGGGGTG TGCTCCTCCT GGCTCAACAG AATACAGGCT 
GACGATGTAG TCCCCTGCTT CGTGAGAGGT GCCCCTAGCT TCCACCTGCC TCGAAACCCC 
CAGGTGCCTT GCATCCTGGT TGGCCCAGGC ACTGGCATCG CACCCTTCCG AAGCTTCTGG 
CAACAGCGAC AATTTGACAT CCAACACAAA GGAATGAATC CGTGCCCCAT GGTTCTGGTC 
TTTGGGTGTC GACAATCCAA GATAGATCAT ATCTACAGAG AGGAGACCCT GCAGGCCAAG 
AACAAGGGCG TCTTCAGAGA GCTGTACACT GCCTATTCCC GGGAACCGGA CAGGCCAAAG 
AAATATGTAC AGGACGTGCT GCAGGAACAG CTGGCTGAGT CTGTGTACCG CGCCCTGAAG 
GAGCAAGGAG GCCACATTTA TGTCTGTGGG GACGTTACCA TGGCCGCCGA TGTCCTCAAA 
GCCATCCAGC GCATAATGAC CCAGCAGGGG AAACTCTCAG AGGAGGACGC TGGTGTATTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CACGAGGACA TCTTTGGAGT CACCCTCAGA 
ACGTATGAAG TGACCAACCG CCTTAGATCT GAGTCCATCG CCTTCATCGA AGAGAGCAAA 
AAAGACGCAG ATGAGGTTTT CAGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mechanism and regulation of ferrous heme-nitric oxide (NO) oxidation in NO synthases.
The Journal of biological chemistry294(19)7904-7916(2019 05)
Tejero J,Hunt AP,Santolini J,Lehnert N,Stuehr DJ

Effect of TRPV1 on Activity of Isoforms of Constitutive Nitric Oxide Synthase during Regulation of Bicarbonate Secretion in the Stomach.
Bulletin of experimental biology and medicine166(3)310-312(2019 Jan)
Zolotarev VA,Andreeva YV,Khropycheva RP

The essential role of hypothalamic paraventricular nucleus nNOS in the modulation of autonomic control in exercised rats.
Nitric oxide : biology and chemistry7914-24(2018 09)
Raquel HA,Ferreira NZ,Lucchetti BFC,Falquetto B,Pinge-Filho P,Michelini LC,Martins-Pinge MC

PSD95 and nNOS interaction as a novel molecular target to modulate conditioned fear: relevance to PTSD.
Translational psychiatry8(1)155(2018 08)
Li LP,Dustrude ET,Haulcomb MM,Abreu AR,Fitz SD,Johnson PL,Thakur GA,Molosh AI,Lai Y,Shekhar A

Nitric oxide in skeletal muscle.
Nature372(6506)546-8(1994 Dec)
Kobzik L,Reid MB,Bredt DS,Stamler JS

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Nos1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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