notch2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	notch2
Entrez Gene ID	100486344
Full Name	notch 2
Synonyms	ags2
General protein information	Preferred Names notch 2 Names neurogenic locus notch homolog protein 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002939080.5	XP_002939126.3	neurogenic locus notch homolog protein 2
XM_002939080.4	XP_002939126.2	neurogenic locus notch homolog protein 2

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	NOTCH2	XP_001114248.1
Rattus norvegicus (Norway rat)	Notch2	NP_077334.1
Homo sapiens (human)	NOTCH2	NP_077719.2
Canis lupus familiaris (dog)	NOTCH2	XP_005630745.1
Bos taurus (cattle)	NOTCH2	XP_002686160.2
Xenopus tropicalis (tropical clawed frog)	LOC100486344	XP_002939126.2
Pan troglodytes (chimpanzee)	NOTCH2	XP_513697.3
Mus musculus (house mouse)	Notch2	NP_035058.2
Gallus gallus (chicken)	NOTCH2	NP_001238962.1
Danio rerio (zebrafish)	notch2	NP_001108566.1

Gene Ontology

The following notch2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the notch2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa09185	XM_002939080.4 Latest version!	Xenopus tropicalis notch 2 (notch2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa57387	XM_002939080.5 Latest version!	Xenopus tropicalis notch 2 (notch2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

notch2 ( XM_002939080.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939126.2 Xenopus tropicalis notch 2 (notch2), mRNA.

Price & Availability↑

CloneID	OXa09185
Clone ID Related Accession (Same CDS sequence)	XM_002939080.4
Accession Version	XM_002939080.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neurogenic locus notch homolog protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939080.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCGC AGTATGTGCT GGGGCTGTGT GTCCTGCTGC TGGGGATGCC GGGCTGTGAC 
GGCTTACAGT GTCGAGGTGC CAGTGAGCCA TGCTTAAATG AAGGAAAATG TATCACCAAT 
GATGACGGCT CTGCATACTG CAAATGCACA GATGCATTTG TTGGAAAATA CTGCCAACTG 
CAAAACCCTT GCCAAGAAAC CATATGCCTC AATGGCGGCA CCTGCAAAGT CAGCTCTGAC 
CTGTCTAAAG GTGTGTGTAC TTGCGCCCCA GGATTCGGCG GGGAGAACTG CAAGCAGCCA 
GTTGCCAATG TCTGCTCCCT TACTCAGCCT TGTATGAACG GGGGCACCTG CCGCCTGCTT 
ACTCTGGAAC TCTATGACTG TATCTGCCCC TTTGGATGGA GAGGAAATGA TTGCAAATTC 
ATTGATGCCT GTGCCTCTCA GCCATGCGCC AACGGCAGTA CCTGCATCCC CAATGAGAAC 
CAGTATACCT GTTCTTGTCA GGCTGGATAC ACCGGCCTCA AGTGTGAAGT GGATGTGAAC 
GAGTGTGGCC CGCCAGGGAT GTGCAAAAAT GGAGGAACTT GTGAGAATCT TCCTGGGTCC 
TACAGGTGTC AGTGTCACCC GGGTTACACT GGTCAGCGCT GTGAGAGCCC CTTCATCCCC 
TGCTCTCCTT CCCCATGTAT GAATGGTGGC ACCTGCCGTC AGACTAGTGA ATTTGCCTAT 
GAATGCAACT GCCTGCCAGG ATTTGATGGG AAAAACTGTG AAACCAACAT AGATGATTGT 
CCCAGTCATA AGTGCCAGAA TGGTGGAACC TGTGTGGATG GGGTGAACAC CTACAACTGC 
AGGTGTCCTC CCCAGTGGAC TGGCCAGTTT TGCACAGAGG ATGTTGATGA GTGCCAACTC 
CAGCCCAATG CTTGCCAAAA TGGTGGGACT TGTACCAACA GCAATGGAGG CTACAACTGC 
GTCTGTGTAA ATGGATGGAG TGGGGATGAT TGCAGCCAGA ATATCGATGA CTGTGCCACT 
GCTGCCTGTG CCAATGGGTC TACTTGTATT GATCGAGTTG CTTCCTTTAT ATGTGTTTGC 
CCAGAAGGAA AGATTGGACT CCTGTGTCAC AAAAATGATG CCTGCTTTAG TAATCCTTGC 
CACATGGGCT CTTTGTGTGA TACCAACCCT CTGACTGGAC AAGCCATTTG CACTTGCCCC 
TCTGGGTACA AAGGGACTGC CTGCACAGAG GACATAGATG AATGCTCTTT GGTTAACAGC 
AATCCCTGCG AGCATGCAGG AAAATGTGTC AATACTGAAG GTTCCTTTTA TTGTGAATGC 
TCCAAAGGAT TCACCGGGCC CCGCTGTGAA ATGGACATTA ATGAGTGCCT ATCTGAGCCA 
TGCCAGAATG ATGCCACCTG TCTGGATAAA ATCGGAGGCT TCACATGTTT GTGTATGCCA 
GGTTACAAGG GAATACACTG TGAAATTGAG ATCAATGAAT GCCTAAGCAA CCCCTGTGTG 
AACAATGGAG AGTGTGTTGA CAAAGTGAAT CGCTTTGTGT GCATTTGCCC TCCAGGTTTC 
ACTGGATCAG TGTGCCAGAT TGATATAGAC GACTGCTCTA GCACTCCATG TCAGAATGGT 
GCCAAATGCG TTGATCATCC ATCTGGCTAT GACTGTATAT GTGCAACAGG CTTCACAGGT 
GTTTTGTGCG AAGAAAATAT CAACAACTGT GAACCACCAC CCTGTCATTA TGGAACATGC 
CAAGATGGCA TTGATTCCTA TACGTGCATT TGTGAGCCTG GCTATATGGG TGCCATTTGC 
AGCGACCAGA TCGATGAATG CCTGAGTAAC CCTTGTCTCA ATGAAGGCCG CTGTGTTGAC 
CTTGTCAATG GCTATCAGTG TAACTGCCTA CCTGGAACAT CTGGGCCAAG ATGTGAGAAG 
AATGTTGATG ACTGTGCAAG CAACCCGTGC ACCTATGGGA ACTGTGTGGA TGGGATAAAC 
CGCTACGACT GTGTCTGCTC CCCAGGATTT ACAGGCCCTC AGTGTAAAGA TGACATTGAT 
GAGTGTGCCT CAAACCCCTG CCATAATGGA GGAACGTGCG TAAATGAACA GAACAGATTT 
CGCTGTATCT GTCCGGAGGG ATTTCGCCAG CCCAGCTGTT TTTCGCAGGT GGACGAATGT 
GTCAATAACC CATGTATCCA CGGCAACTGC ACAGATGATG TCAATGGGTA CAAGTGTCTC 
TGCCAACCAG GCTGGACAGG CAAGAAATGC GAGGTGGATA AAAACGAGTG TCTTTCCAAC 
CCCTGTCAGA ATGGGGGGAC ATGCGATAAC CTGGTGAATG GATACAGATG CTCCTGCAAG 
AAAGGGTTCA AGGGTTACAA CTGTCAGATT GACATCGATG AATGTGCTTC AAATCCATGT 
TTAAACCATG GGACGTGCAG AGATGGGATC AATGGTTACA CGTGCCACTG CGGGCTGCCG 
TATACTGGTA CAAACTGCCA GAACATTCTG ACCCCCTGTA GCCCTGAACC GTGCCACAAT 
GGTGGAATTT GTCAGGAATC TGAAGATTTC AAGAGTTTCA GCTGTCTGTG TGCAACTGGC 
TGGCACGGTC AGAGGTGTAC TATTGATATT GATGAATGTA TAACCAACCC CTGCAAAAAC 
TATGGTCGCT GCCAAAATAC CAAAGGCGGA TACATTTGCC ATTGCTTTGA AGGCTTTAAT 
GGAGTCAACT GTGAAAACAA CATTGACGAT TGCCTTTCTA ATCCGTGTCA GAATGGGGCT 
TCCTGTGTAG ATGGAATCAA CACTTTCTCT TGTAACTGCC TGGCGGGGTT CCATGGGGAC 
AAGTGCCAGA AAGATGTGGA TGAATGTGCA AGCAACCCTT GTAAAAATGG AGGCTCCTGC 
ACAGACTACG TCAACAGCTA CACGTGCAAG TGTCAGCCGG GATTTGATGG AATCCACTGT 
GATAACAACA TTGATGAATG CACAGACACG TCCTGCTTCA ATGGTGCTAC CTGCGTGGAT 
GGCATTAATT CCTTCACCTG CCAGTGCCCT CAGGGATTTA CAGGACAATT TTGTCTGTTT 
GAGATCAATG AGTGTGGGTC CCACCCCTGC ATTAATGGAG GCACTTGTGT GGATGGAATG 
GGAACATATC ACTGCACTTG TCCTGTCGGA TACAGAGGCA AGAACTGTGA GGAGTTGGTT 
GATCTGTGCA GTGGCTTTCC ATGTAAAAAC AGAGGCATCT GCAAGCAAGT GAAAACAGAA 
CCCAAATGCA TTTGTCCAAC GGGTTGGACT GGCATTTACT GTGATATCCC AGATGTCTCT 
TGTGAAGTGG CTGCGTCTCA GAGGGGTGTG GCAGTGCAAG ATCTATGTCA GCATGCTGGC 
ATTTGCATCA ATACTGGCTT CAGTCACCGT TGCCAGTGCC GACAAGGCTA CATCGGCAGT 
TACTGTGAGG GGGAACTGGA TGAGTGTGCC TCCAATCCAT GCCAGAATAG CGCTACTTGT 
GTGGATCGTC ATGGGGGATA CGAGTGCAAG TGCTTGCCAG GGTACCAAGG AGTAAACTGC 
GAGTATGAGA TAGACGAGTG CCAGATCCAG CCTTGCCACA ATGGTGGGAC ATGTGTTGAC 
TTGGTAAATC AATTCCGATG CTCCTGCCCT CCAGGAACAC GAGGTCCCCT CTGTGAAGAA 
AATATAGATG ACTGTGCTCC AAGTTCTGAA GGGCCGCACT GTTTCAATGG TGGAAGGTGC 
TTAGATAAAA TTGGCGGCTA CAGCTGTGAA TGCCTCCCTG GGTTTGCAGG GGAGAGGTGT 
GAAGGCGATG TGAATGAATG CTTGTCCAAT CCTTGTAGTG CAAAGGGCAG CCTGGACTGC 
ATTCAGCTGG ATAACAACTA TCTTTGCAAG TGCAAGCCTG GATTCACAGG TCGCCACTGT 
GAGACGATAC AAGATTTGTG CCCAACTCGA CCTTGTCTGA ATGGTGGGAC ATGCGCTGTT 
GCTGTGAATA TGCCTGAAGG ATTTACCTGT CAGTGTCCAC CAGGCTTTTC TGGTTCTAAA 
TGCCAGTACA GTAATAATAC ATGTGGACAC TATAAGTGCA AAGTTGGGGA AAACTGTATT 
AAAACTCCTT TGGGACCAAG GTGCTACTGT CCCACGGGAC CAGCTGGGAA TGCATGCCAA 
TCTAAATTAG GCTGTACCAG CCAGCCCTGT AAAAACAATG GAGTTTGCCA GAGAAAGGAT 
CAGCCTCCCT ATTATACATG CAATTGCCCT AATGGAATTG TTGGACTCCA GTGTGAAATT 
CTATTGATTG ATGGGTGTCA CCTCCACCAC TGTCCTGATA AAGCAAGAGA CGGGTACTGC 
GATCAGGAGT GTAACACGCA TGAATGCCTT TGGGATGGTG GAGACTGCTC TTTAACAATG 
GAAGACCCAT GGGCCAACTG TTCCTCCTCA TTGAAATGCT GGGAATATTT TAATGGCCAA 
TGTGATGAGC TGTGTAATAC ACCTGAGTGC CTGTTTGACA ACTTTGAGTG TCAAGAAAAA 
GAGTCAATCT GCAAGTATGA TAAGTACTGT GAAGATCACT ATGCAAATGG TCACTGTGAC 
AAAAGCTGTA ACACAAAAGA ATGTGGCTGG GATGGACTCG ACTGCTCTAC AGACAAACCT 
GAGAATCTTG CTGAGGGTAC CTTGGTCATA GTAGTGCTAA TGCCCCCAAA GGAACTGCTA 
AAAAATTCCA GAAATTTCCT CCGGGAGTTA GGCAGCATTC TTCGCACCAA CCTAAGGATC 
AAAAGGAATC CAGATGGGAG CCCCATGGTC TTTCCATATT TTAGAGAAAA ATCAAGCGCA 
CAGCGTTCAC GTAGATCTCT CTTAGAATTA CGCGTAGTCC GGGAGCTTGA GGAGCAGCCA 
ATTGGCTCAA AAGTCTCATT AGAAATTGAC AACCAACAGT GTGTCCTGGA TTCAGATCAG 
TGCTTTAAGA CTACAGACTC CGCTGCTGCC ATGCTGGCTG CCCATGCTGT ACAGGGTACA 
CTTAATTATC CCCTGGTCTC AGTGGGGGTT GAAAAGGCGC CACCATCGTC CCAGACACTG 
ATGTACATAT TGCCCGTTAT ATTGGCCATC ATTATTGTCA TTCTGCTGCT GGTTGTGGTA 
CTGGCCAAAA AGAAGCGCAA ACATGGCACC CTTTGGTTCC CTGAAGGTTT CCTCCTGCAC 
CGGGACACCA GCAACCAGAA GCGCAGAGAG CCTGTTGGGC AGGATGCTGT TGGAATGAAA 
AATTTGTCTG TGCCCATAGT GGATGGAAAC TTACTGGATT CTAATCAGAA TGATCACTGG 
TCAGATGATC GAGGACCACA GCCAAAGAAA TCCAAGTCTG AAGGTCAGGC TCTCCTGTCG 
GGAGGAGATG ACCAAGTTGA TAGCCGTCAG TGGACACAGC AGCACCTGGT GGCTGCAGAT 
ATTCGGATGA CCCCATCATT GGCTCTCACA CCTCCTCAAG CAGAGCAAGA CATGGATGTC 
ATAGATGTCA ACGTGCGAGG GCCAGATGGC TGCACACCAC TTATGTTGGC CTCCCTGCGA 
ATGGGAAGCC CTCACATAGA GGAAGATGAT GAGGAGGCAG AAGATCCATC TGCTAACGTC 
ATTACTGACC TGATTTATCA GGGAGCTTGT TTGCAGGCCC AGACAGATCG CACAGGAGAA 
ACTGCCCTGC ACCTGGCAGC TCGCTACTCA AGGGCCGATG CTGCCAAGCG ATTGCTGGAT 
GCTGGAGCTG ATGCCAACTC TCAGGACAGT ATGGGGCGGA CCCCTCTACA TGCAGCGGTC 
TCTGCTGATG CACAGGGGGT TTTCCAGATT TTGATCCGTA ACAGAGTCAC AGACCTCGAT 
GCGAGGATGA ATGATGGTAC AACCCCTCTC ATCTTAGCTG CCCGCCTTGC TGTGGAAGGG 
ATGGTTGCAG ACTTAATAAA CTGCCAGGCT GATGTTAATG CAGTGGATGA TCATGGTAAA 
TCTGCACTTC ACTGGGCTGC TGCAGTAAAC AATGTTGAGG CTACAATTGT TCTGCTAAAG 
AATGGAGCAA ATAGGGATAT GCAAGACAAC AAGGAAGAAA CACCCTTGTT TCTTGCTGCT 
AGAGAGGGTA GTTTTGAAGC AGCCAAAATG CTTTTGGATT ACTTTGCAAA CCGAGAAATC 
ACTGACCATA TGGATCGTCT TCCCCGTGAC ATTGCCAAGG ACAGAATGCA TCATGACATT 
GTGCGCCTGT TGGACGAGTA TAACTTGGTG CACAGCCCAC AAGGACAGCC TGGTACAATG 
CTTTCAAATT CCCTGTCGCC AGCAGTGTGT GGCTCCAACA GATCTTTCCT GAACCTGAAA 
CATGCCGCAC AAACAAAGAA GGCCAGAAGG CCAAGTGCCA AAAGTGTAGC ACCTCGAAGT 
CTTCCAAACT TAGCCAAAGA ACCAAAAGAT ACCAAAAACA GGAGGAGGAA AAAGTCAGAT 
ACTGACAGTA AAGCGCAGCT TTCTGAAAGC TCAGTAACAT TATCCCCTGT GGATTCTCTT 
GAATCCCCCC ATGCTACTTC ATCTCCTACC ATTACATCTC CTGGAGCGCT GCATACGTCC 
CCTGGACAGA TGATTGCAGC CTCTACGTTG CAGCCACCAC GCACAACAAT GTCTTTAACT 
AATCTTCAGC CCGTTGGTTG TGCAGCCAAT AATATGCTGC CTTCTGTTGG ACAGTTGTTA 
TCACACTCGG CTCAAAATAA TGTTCTTACA GCACAAAGAC AGCTCACTAT GTCATCTGAT 
TGGCTGAGCC GCATGGAAAG GAATGACACC CAGTATAATG AAATGTTTGG TATAGTCCAC 
CATGACACAA ATAACCACTC ATGTATTCCT CAACAAAATG GTATTATCCA AGCAGATATC 
AAGCCAACAC TGCATATGGG TGTGGCTCGG GATGCTTTGC CTCCTATTAT GACTTTCCAG 
CTTATCCCAA AGAGTGGAAT GAACCAGCAG TCTGGCATCC AGACACTTGT GCAGCAGGCT 
AGTCATGGTC AGAATGTGGC TGCCATGTAT CAGATTCCTG ATAGAGCACG GATCACTGGC 
TCTTCTGTTC CTATAGCAAT GATGCATCAA CATGATGGAC AGTTATCTCA AACTATGCTC 
ACAGCTTACC ATCCCCTTCA GAACCCAATG GGAAAATATC CAACCCCACC ATCACAACAC 
AGCTACACTT CCACTGATAA AACCCCAAAC CATAATGGAC ATTTACCCGG AGAGCACCCT 
TACTTGACCC CTTCACCAGA ATCTCCAGAT CAGTGGTCCA GCTCCTCACC TAACTCTGCC 
TCTGATTGGT CAGATGTAAC TACAAGTCCG ACCCCTAATG GCAATCAAAG AATGCCTGCC 
ACACATATGC CTGAACAATC ACACAATGGC ATGCAGATTT ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939126.2
CDS	495..7862
Translation	MGAQYVLGLCVLLLGMPGCDGLQCRGASEPCLNEGKCITNDDGSAYCKCTDAFVGKYCQLQNPCQETICLNGGTCKVSSDLSKGVCTCAPGFGGENCKQPVANVCSLTQPCMNGGTCRLLTLELYDCICPFGWRGNDCKFIDACASQPCANGSTCIPNENQYTCSCQAGYTGLKCEVDVNECGPPGMCKNGGTCENLPGSYRCQCHPGYTGQRCESPFIPCSPSPCMNGGTCRQTSEFAYECNCLPGFDGKNCETNIDDCPSHKCQNGGTCVDGVNTYNCRCPPQWTGQFCTEDVDECQLQPNACQNGGTCTNSNGGYNCVCVNGWSGDDCSQNIDDCATAACANGSTCIDRVASFICVCPEGKIGLLCHKNDACFSNPCHMGSLCDTNPLTGQAICTCPSGYKGTACTEDIDECSLVNSNPCEHAGKCVNTEGSFYCECSKGFTGPRCEMDINECLSEPCQNDATCLDKIGGFTCLCMPGYKGIHCEIEINECLSNPCVNNGECVDKVNRFVCICPPGFTGSVCQIDIDDCSSTPCQNGAKCVDHPSGYDCICATGFTGVLCEENINNCEPPPCHYGTCQDGIDSYTCICEPGYMGAICSDQIDECLSNPCLNEGRCVDLVNGYQCNCLPGTSGPRCEKNVDDCASNPCTYGNCVDGINRYDCVCSPGFTGPQCKDDIDECASNPCHNGGTCVNEQNRFRCICPEGFRQPSCFSQVDECVNNPCIHGNCTDDVNGYKCLCQPGWTGKKCEVDKNECLSNPCQNGGTCDNLVNGYRCSCKKGFKGYNCQIDIDECASNPCLNHGTCRDGINGYTCHCGLPYTGTNCQNILTPCSPEPCHNGGICQESEDFKSFSCLCATGWHGQRCTIDIDECITNPCKNYGRCQNTKGGYICHCFEGFNGVNCENNIDDCLSNPCQNGASCVDGINTFSCNCLAGFHGDKCQKDVDECASNPCKNGGSCTDYVNSYTCKCQPGFDGIHCDNNIDECTDTSCFNGATCVDGINSFTCQCPQGFTGQFCLFEINECGSHPCINGGTCVDGMGTYHCTCPVGYRGKNCEELVDLCSGFPCKNRGICKQVKTEPKCICPTGWTGIYCDIPDVSCEVAASQRGVAVQDLCQHAGICINTGFSHRCQCRQGYIGSYCEGELDECASNPCQNSATCVDRHGGYECKCLPGYQGVNCEYEIDECQIQPCHNGGTCVDLVNQFRCSCPPGTRGPLCEENIDDCAPSSEGPHCFNGGRCLDKIGGYSCECLPGFAGERCEGDVNECLSNPCSAKGSLDCIQLDNNYLCKCKPGFTGRHCETIQDLCPTRPCLNGGTCAVAVNMPEGFTCQCPPGFSGSKCQYSNNTCGHYKCKVGENCIKTPLGPRCYCPTGPAGNACQSKLGCTSQPCKNNGVCQRKDQPPYYTCNCPNGIVGLQCEILLIDGCHLHHCPDKARDGYCDQECNTHECLWDGGDCSLTMEDPWANCSSSLKCWEYFNGQCDELCNTPECLFDNFECQEKESICKYDKYCEDHYANGHCDKSCNTKECGWDGLDCSTDKPENLAEGTLVIVVLMPPKELLKNSRNFLRELGSILRTNLRIKRNPDGSPMVFPYFREKSSAQRSRRSLLELRVVRELEEQPIGSKVSLEIDNQQCVLDSDQCFKTTDSAAAMLAAHAVQGTLNYPLVSVGVEKAPPSSQTLMYILPVILAIIIVILLLVVVLAKKKRKHGTLWFPEGFLLHRDTSNQKRREPVGQDAVGMKNLSVPIVDGNLLDSNQNDHWSDDRGPQPKKSKSEGQALLSGGDDQVDSRQWTQQHLVAADIRMTPSLALTPPQAEQDMDVIDVNVRGPDGCTPLMLASLRMGSPHIEEDDEEAEDPSANVITDLIYQGACLQAQTDRTGETALHLAARYSRADAAKRLLDAGADANSQDSMGRTPLHAAVSADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVADLINCQADVNAVDDHGKSALHWAAAVNNVEATIVLLKNGANRDMQDNKEETPLFLAAREGSFEAAKMLLDYFANREITDHMDRLPRDIAKDRMHHDIVRLLDEYNLVHSPQGQPGTMLSNSLSPAVCGSNRSFLNLKHAAQTKKARRPSAKSVAPRSLPNLAKEPKDTKNRRRKKSDTDSKAQLSESSVTLSPVDSLESPHATSSPTITSPGALHTSPGQMIAASTLQPPRTTMSLTNLQPVGCAANNMLPSVGQLLSHSAQNNVLTAQRQLTMSSDWLSRMERNDTQYNEMFGIVHHDTNNHSCIPQQNGIIQADIKPTLHMGVARDALPPIMTFQLIPKSGMNQQSGIQTLVQQASHGQNVAAMYQIPDRARITGSSVPIAMMHQHDGQLSQTMLTAYHPLQNPMGKYPTPPSQHSYTSTDKTPNHNGHLPGEHPYLTPSPESPDQWSSSSPNSASDWSDVTTSPTPNGNQRMPATHMPEQSHNGMQIYA

Target ORF information:

RefSeq Version	XM_002939080.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis notch 2 (notch2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939080.4

ORF Insert Sequence:

ATGGGGGCGC AGTATGTGCT GGGGCTGTGT GTCCTGCTGC TGGGGATGCC GGGCTGTGAC 
GGCTTACAGT GTCGAGGTGC CAGTGAGCCA TGCTTAAATG AAGGAAAATG TATCACCAAT 
GATGACGGCT CTGCATACTG CAAATGCACA GATGCATTTG TTGGAAAATA CTGCCAACTG 
CAAAACCCTT GCCAAGAAAC CATATGCCTC AATGGCGGCA CCTGCAAAGT CAGCTCTGAC 
CTGTCTAAAG GTGTGTGTAC TTGCGCCCCA GGATTCGGCG GGGAGAACTG CAAGCAGCCA 
GTTGCCAATG TCTGCTCCCT TACTCAGCCT TGTATGAACG GGGGCACCTG CCGCCTGCTT 
ACTCTGGAAC TCTATGACTG TATCTGCCCC TTTGGATGGA GAGGAAATGA TTGCAAATTC 
ATTGATGCCT GTGCCTCTCA GCCATGCGCC AACGGCAGTA CCTGCATCCC CAATGAGAAC 
CAGTATACCT GTTCTTGTCA GGCTGGATAC ACCGGCCTCA AGTGTGAAGT GGATGTGAAC 
GAGTGTGGCC CGCCAGGGAT GTGCAAAAAT GGAGGAACTT GTGAGAATCT TCCTGGGTCC 
TACAGGTGTC AGTGTCACCC GGGTTACACT GGTCAGCGCT GTGAGAGCCC CTTCATCCCC 
TGCTCTCCTT CCCCATGTAT GAATGGTGGC ACCTGCCGTC AGACTAGTGA ATTTGCCTAT 
GAATGCAACT GCCTGCCAGG ATTTGATGGG AAAAACTGTG AAACCAACAT AGATGATTGT 
CCCAGTCATA AGTGCCAGAA TGGTGGAACC TGTGTGGATG GGGTGAACAC CTACAACTGC 
AGGTGTCCTC CCCAGTGGAC TGGCCAGTTT TGCACAGAGG ATGTTGATGA GTGCCAACTC 
CAGCCCAATG CTTGCCAAAA TGGTGGGACT TGTACCAACA GCAATGGAGG CTACAACTGC 
GTCTGTGTAA ATGGATGGAG TGGGGATGAT TGCAGCCAGA ATATCGATGA CTGTGCCACT 
GCTGCCTGTG CCAATGGGTC TACTTGTATT GATCGAGTTG CTTCCTTTAT ATGTGTTTGC 
CCAGAAGGAA AGATTGGACT CCTGTGTCAC AAAAATGATG CCTGCTTTAG TAATCCTTGC 
CACATGGGCT CTTTGTGTGA TACCAACCCT CTGACTGGAC AAGCCATTTG CACTTGCCCC 
TCTGGGTACA AAGGGACTGC CTGCACAGAG GACATAGATG AATGCTCTTT GGTTAACAGC 
AATCCCTGCG AGCATGCAGG AAAATGTGTC AATACTGAAG GTTCCTTTTA TTGTGAATGC 
TCCAAAGGAT TCACCGGGCC CCGCTGTGAA ATGGACATTA ATGAGTGCCT ATCTGAGCCA 
TGCCAGAATG ATGCCACCTG TCTGGATAAA ATCGGAGGCT TCACATGTTT GTGTATGCCA 
GGTTACAAGG GAATACACTG TGAAATTGAG ATCAATGAAT GCCTAAGCAA CCCCTGTGTG 
AACAATGGAG AGTGTGTTGA CAAAGTGAAT CGCTTTGTGT GCATTTGCCC TCCAGGTTTC 
ACTGGATCAG TGTGCCAGAT TGATATAGAC GACTGCTCTA GCACTCCATG TCAGAATGGT 
GCCAAATGCG TTGATCATCC ATCTGGCTAT GACTGTATAT GTGCAACAGG CTTCACAGGT 
GTTTTGTGCG AAGAAAATAT CAACAACTGT GAACCACCAC CCTGTCATTA TGGAACATGC 
CAAGATGGCA TTGATTCCTA TACGTGCATT TGTGAGCCTG GCTATATGGG TGCCATTTGC 
AGCGACCAGA TCGATGAATG CCTGAGTAAC CCTTGTCTCA ATGAAGGCCG CTGTGTTGAC 
CTTGTCAATG GCTATCAGTG TAACTGCCTA CCTGGAACAT CTGGGCCAAG ATGTGAGAAG 
AATGTTGATG ACTGTGCAAG CAACCCGTGC ACCTATGGGA ACTGTGTGGA TGGGATAAAC 
CGCTACGACT GTGTCTGCTC CCCAGGATTT ACAGGCCCTC AGTGTAAAGA TGACATTGAT 
GAGTGTGCCT CAAACCCCTG CCATAATGGA GGAACGTGCG TAAATGAACA GAACAGATTT 
CGCTGTATCT GTCCGGAGGG ATTTCGCCAG CCCAGCTGTT TTTCGCAGGT GGACGAATGT 
GTCAATAACC CATGTATCCA CGGCAACTGC ACAGATGATG TCAATGGGTA CAAGTGTCTC 
TGCCAACCAG GCTGGACAGG CAAGAAATGC GAGGTGGATA AAAACGAGTG TCTTTCCAAC 
CCCTGTCAGA ATGGGGGGAC ATGCGATAAC CTGGTGAATG GATACAGATG CTCCTGCAAG 
AAAGGGTTCA AGGGTTACAA CTGTCAGATT GACATCGATG AATGTGCTTC AAATCCATGT 
TTAAACCATG GGACGTGCAG AGATGGGATC AATGGTTACA CGTGCCACTG CGGGCTGCCG 
TATACTGGTA CAAACTGCCA GAACATTCTG ACCCCCTGTA GCCCTGAACC GTGCCACAAT 
GGTGGAATTT GTCAGGAATC TGAAGATTTC AAGAGTTTCA GCTGTCTGTG TGCAACTGGC 
TGGCACGGTC AGAGGTGTAC TATTGATATT GATGAATGTA TAACCAACCC CTGCAAAAAC 
TATGGTCGCT GCCAAAATAC CAAAGGCGGA TACATTTGCC ATTGCTTTGA AGGCTTTAAT 
GGAGTCAACT GTGAAAACAA CATTGACGAT TGCCTTTCTA ATCCGTGTCA GAATGGGGCT 
TCCTGTGTAG ATGGAATCAA CACTTTCTCT TGTAACTGCC TGGCGGGGTT CCATGGGGAC 
AAGTGCCAGA AAGATGTGGA TGAATGTGCA AGCAACCCTT GTAAAAATGG AGGCTCCTGC 
ACAGACTACG TCAACAGCTA CACGTGCAAG TGTCAGCCGG GATTTGATGG AATCCACTGT 
GATAACAACA TTGATGAATG CACAGACACG TCCTGCTTCA ATGGTGCTAC CTGCGTGGAT 
GGCATTAATT CCTTCACCTG CCAGTGCCCT CAGGGATTTA CAGGACAATT TTGTCTGTTT 
GAGATCAATG AGTGTGGGTC CCACCCCTGC ATTAATGGAG GCACTTGTGT GGATGGAATG 
GGAACATATC ACTGCACTTG TCCTGTCGGA TACAGAGGCA AGAACTGTGA GGAGTTGGTT 
GATCTGTGCA GTGGCTTTCC ATGTAAAAAC AGAGGCATCT GCAAGCAAGT GAAAACAGAA 
CCCAAATGCA TTTGTCCAAC GGGTTGGACT GGCATTTACT GTGATATCCC AGATGTCTCT 
TGTGAAGTGG CTGCGTCTCA GAGGGGTGTG GCAGTGCAAG ATCTATGTCA GCATGCTGGC 
ATTTGCATCA ATACTGGCTT CAGTCACCGT TGCCAGTGCC GACAAGGCTA CATCGGCAGT 
TACTGTGAGG GGGAACTGGA TGAGTGTGCC TCCAATCCAT GCCAGAATAG CGCTACTTGT 
GTGGATCGTC ATGGGGGATA CGAGTGCAAG TGCTTGCCAG GGTACCAAGG AGTAAACTGC 
GAGTATGAGA TAGACGAGTG CCAGATCCAG CCTTGCCACA ATGGTGGGAC ATGTGTTGAC 
TTGGTAAATC AATTCCGATG CTCCTGCCCT CCAGGAACAC GAGGTCCCCT CTGTGAAGAA 
AATATAGATG ACTGTGCTCC AAGTTCTGAA GGGCCGCACT GTTTCAATGG TGGAAGGTGC 
TTAGATAAAA TTGGCGGCTA CAGCTGTGAA TGCCTCCCTG GGTTTGCAGG GGAGAGGTGT 
GAAGGCGATG TGAATGAATG CTTGTCCAAT CCTTGTAGTG CAAAGGGCAG CCTGGACTGC 
ATTCAGCTGG ATAACAACTA TCTTTGCAAG TGCAAGCCTG GATTCACAGG TCGCCACTGT 
GAGACGATAC AAGATTTGTG CCCAACTCGA CCTTGTCTGA ATGGTGGGAC ATGCGCTGTT 
GCTGTGAATA TGCCTGAAGG ATTTACCTGT CAGTGTCCAC CAGGCTTTTC TGGTTCTAAA 
TGCCAGTACA GTAATAATAC ATGTGGACAC TATAAGTGCA AAGTTGGGGA AAACTGTATT 
AAAACTCCTT TGGGACCAAG GTGCTACTGT CCCACGGGAC CAGCTGGGAA TGCATGCCAA 
TCTAAATTAG GCTGTACCAG CCAGCCCTGT AAAAACAATG GAGTTTGCCA GAGAAAGGAT 
CAGCCTCCCT ATTATACATG CAATTGCCCT AATGGAATTG TTGGACTCCA GTGTGAAATT 
CTATTGATTG ATGGGTGTCA CCTCCACCAC TGTCCTGATA AAGCAAGAGA CGGGTACTGC 
GATCAGGAGT GTAACACGCA TGAATGCCTT TGGGATGGTG GAGACTGCTC TTTAACAATG 
GAAGACCCAT GGGCCAACTG TTCCTCCTCA TTGAAATGCT GGGAATATTT TAATGGCCAA 
TGTGATGAGC TGTGTAATAC ACCTGAGTGC CTGTTTGACA ACTTTGAGTG TCAAGAAAAA 
GAGTCAATCT GCAAGTATGA TAAGTACTGT GAAGATCACT ATGCAAATGG TCACTGTGAC 
AAAAGCTGTA ACACAAAAGA ATGTGGCTGG GATGGACTCG ACTGCTCTAC AGACAAACCT 
GAGAATCTTG CTGAGGGTAC CTTGGTCATA GTAGTGCTAA TGCCCCCAAA GGAACTGCTA 
AAAAATTCCA GAAATTTCCT CCGGGAGTTA GGCAGCATTC TTCGCACCAA CCTAAGGATC 
AAAAGGAATC CAGATGGGAG CCCCATGGTC TTTCCATATT TTAGAGAAAA ATCAAGCGCA 
CAGCGTTCAC GTAGATCTCT CTTAGAATTA CGCGTAGTCC GGGAGCTTGA GGAGCAGCCA 
ATTGGCTCAA AAGTCTCATT AGAAATTGAC AACCAACAGT GTGTCCTGGA TTCAGATCAG 
TGCTTTAAGA CTACAGACTC CGCTGCTGCC ATGCTGGCTG CCCATGCTGT ACAGGGTACA 
CTTAATTATC CCCTGGTCTC AGTGGGGGTT GAAAAGGCGC CACCATCGTC CCAGACACTG 
ATGTACATAT TGCCCGTTAT ATTGGCCATC ATTATTGTCA TTCTGCTGCT GGTTGTGGTA 
CTGGCCAAAA AGAAGCGCAA ACATGGCACC CTTTGGTTCC CTGAAGGTTT CCTCCTGCAC 
CGGGACACCA GCAACCAGAA GCGCAGAGAG CCTGTTGGGC AGGATGCTGT TGGAATGAAA 
AATTTGTCTG TGCCCATAGT GGATGGAAAC TTACTGGATT CTAATCAGAA TGATCACTGG 
TCAGATGATC GAGGACCACA GCCAAAGAAA TCCAAGTCTG AAGGTCAGGC TCTCCTGTCG 
GGAGGAGATG ACCAAGTTGA TAGCCGTCAG TGGACACAGC AGCACCTGGT GGCTGCAGAT 
ATTCGGATGA CCCCATCATT GGCTCTCACA CCTCCTCAAG CAGAGCAAGA CATGGATGTC 
ATAGATGTCA ACGTGCGAGG GCCAGATGGC TGCACACCAC TTATGTTGGC CTCCCTGCGA 
ATGGGAAGCC CTCACATAGA GGAAGATGAT GAGGAGGCAG AAGATCCATC TGCTAACGTC 
ATTACTGACC TGATTTATCA GGGAGCTTGT TTGCAGGCCC AGACAGATCG CACAGGAGAA 
ACTGCCCTGC ACCTGGCAGC TCGCTACTCA AGGGCCGATG CTGCCAAGCG ATTGCTGGAT 
GCTGGAGCTG ATGCCAACTC TCAGGACAGT ATGGGGCGGA CCCCTCTACA TGCAGCGGTC 
TCTGCTGATG CACAGGGGGT TTTCCAGATT TTGATCCGTA ACAGAGTCAC AGACCTCGAT 
GCGAGGATGA ATGATGGTAC AACCCCTCTC ATCTTAGCTG CCCGCCTTGC TGTGGAAGGG 
ATGGTTGCAG ACTTAATAAA CTGCCAGGCT GATGTTAATG CAGTGGATGA TCATGGTAAA 
TCTGCACTTC ACTGGGCTGC TGCAGTAAAC AATGTTGAGG CTACAATTGT TCTGCTAAAG 
AATGGAGCAA ATAGGGATAT GCAAGACAAC AAGGAAGAAA CACCCTTGTT TCTTGCTGCT 
AGAGAGGGTA GTTTTGAAGC AGCCAAAATG CTTTTGGATT ACTTTGCAAA CCGAGAAATC 
ACTGACCATA TGGATCGTCT TCCCCGTGAC ATTGCCAAGG ACAGAATGCA TCATGACATT 
GTGCGCCTGT TGGACGAGTA TAACTTGGTG CACAGCCCAC AAGGACAGCC TGGTACAATG 
CTTTCAAATT CCCTGTCGCC AGCAGTGTGT GGCTCCAACA GATCTTTCCT GAACCTGAAA 
CATGCCGCAC AAACAAAGAA GGCCAGAAGG CCAAGTGCCA AAAGTGTAGC ACCTCGAAGT 
CTTCCAAACT TAGCCAAAGA ACCAAAAGAT ACCAAAAACA GGAGGAGGAA AAAGTCAGAT 
ACTGACAGTA AAGCGCAGCT TTCTGAAAGC TCAGTAACAT TATCCCCTGT GGATTCTCTT 
GAATCCCCCC ATGCTACTTC ATCTCCTACC ATTACATCTC CTGGAGCGCT GCATACGTCC 
CCTGGACAGA TGATTGCAGC CTCTACGTTG CAGCCACCAC GCACAACAAT GTCTTTAACT 
AATCTTCAGC CCGTTGGTTG TGCAGCCAAT AATATGCTGC CTTCTGTTGG ACAGTTGTTA 
TCACACTCGG CTCAAAATAA TGTTCTTACA GCACAAAGAC AGCTCACTAT GTCATCTGAT 
TGGCTGAGCC GCATGGAAAG GAATGACACC CAGTATAATG AAATGTTTGG TATAGTCCAC 
CATGACACAA ATAACCACTC ATGTATTCCT CAACAAAATG GTATTATCCA AGCAGATATC 
AAGCCAACAC TGCATATGGG TGTGGCTCGG GATGCTTTGC CTCCTATTAT GACTTTCCAG 
CTTATCCCAA AGAGTGGAAT GAACCAGCAG TCTGGCATCC AGACACTTGT GCAGCAGGCT 
AGTCATGGTC AGAATGTGGC TGCCATGTAT CAGATTCCTG ATAGAGCACG GATCACTGGC 
TCTTCTGTTC CTATAGCAAT GATGCATCAA CATGATGGAC AGTTATCTCA AACTATGCTC 
ACAGCTTACC ATCCCCTTCA GAACCCAATG GGAAAATATC CAACCCCACC ATCACAACAC 
AGCTACACTT CCACTGATAA AACCCCAAAC CATAATGGAC ATTTACCCGG AGAGCACCCT 
TACTTGACCC CTTCACCAGA ATCTCCAGAT CAGTGGTCCA GCTCCTCACC TAACTCTGCC 
TCTGATTGGT CAGATGTAAC TACAAGTCCG ACCCCTAATG GCAATCAAAG AATGCCTGCC 
ACACATATGC CTGAACAATC ACACAATGGC ATGCAGATTT ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

notch2 ( XM_002939080.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939126.3 Xenopus tropicalis notch 2 (notch2), mRNA.

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CloneID	OXa57387
Clone ID Related Accession (Same CDS sequence)	XM_002939080.5
Accession Version	XM_002939080.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	neurogenic locus notch homolog protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939080.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCGC AGTATGTGCT GGGGCTGTGT GTCCTGCTGC TGGGGATGCC GGGCTGTGAC 
GGCTTACAGT GTCGAGGTGC CAGTGAGCCA TGCTTAAATG AAGGAAAATG TATCACCAAT 
GATGACGGCT CTGCATACTG CAAATGCACA GATGCATTTG TTGGAAAATA CTGCCAACTG 
CAAAACCCTT GCCAAGAAAC CATATGCCTC AATGGCGGCA CCTGTAAAGT CAGCTCTGAC 
CTGTCTAAAG GTGTGTGTAC TTGCGCCCCA GGATTCGGCG GGGAGAACTG CAAGCAGCCA 
GTTGCCAATG TCTGCTCCCT TACTCAGCCT TGTATGAACG GGGGCACCTG CCACCTGCTT 
ACTCTGGAAC TCTATGACTG TATCTGCCCC TTTGGATGGA GAGGAAATGA TTGCAAATTC 
ATTGATGCCT GTGCCTCTCA GCCATGCGCC AACGGCAGTA CCTGCATCCC CAATGAGAAC 
CAGTATACCT GTTCTTGTCA GGCTGGATAC ACCGGCCTCA AGTGTGAAGT GGATGTGAAC 
GAGTGTGGCC CGCCAGGGAT GTGCAAAAAT GGAGGAACTT GTGAGAATTT TCCTGGGTCC 
TACAGGTGTC AGTGTCACCC GGGTTACACT GGTCAGCGCT GTGAGAGCCC CTTCATCCCC 
TGCTCTCCTT CCCCATGTAT GAATGGTGGC ACCTGCCGTC AGACTAGTGA ATTTGCCTAT 
GAATGCAACT GCCTGCCAGG ATTTGATGGG AAAAACTGTG AAACCAACAT AGATGATTGT 
CCCAGTCATA AGTGCCAGAA TGGTGGAACC TGTGTGGATG GGGTGAACAC CTACAACTGC 
AGGTGTCCTC CCCAGTGGAC TGGCCAGTTT TGCACAGAGG ATGTTGATGA GTGCCAACTC 
CAGCCCAATG CTTGCCAAAA TGGTGGGACT TGTACCAACA GCAATGGAGG CTACAACTGC 
GTCTGTGTAA ATGGATGGAG TGGGGATGAT TGCAGCCAGA ATATCGATGA CTGTGCCACT 
GCTGCCTGTG CCAATGGGTC TACTTGTATT GATCGAGTTG CTTCCTTTAT ATGTGTTTGC 
CCAGAAGGAA AGATTGGACT CCTGTGTCAC AAAAATGATG CCTGCTTTAG TAATCCTTGC 
CACATGGGCT CTTTGTGTGA TACCAACCCT CTGACTGGAC AAGCCATTTG CACTTGCCCC 
TCTGGGTACA AAGGGACTGC CTGCACAGAG GACATAGATG AATGCTCTTT GGTTAACAGC 
AATCCCTGTG AGCATGCAGG AAAATGTGTC AATACTGAAG GTTCCTTTTA TTGTGAATGC 
TCCAAAGGAT TCACCGGGCC CCGCTGTGAA ATGGACATTA ATGAGTGCCT ATCTGAGCCA 
TGCCAGAATG ATGCCACCTG TCTGGATAAA ATCGGAGGCT TCACATGTTT GTGTATGCCA 
GGTTACAAGG GAATACACTG TGAAATTGAG ATCAATGAAT GCCTAAGCAA CCCCTGTGTG 
AACAATGGAG AGTGTGTTGA CAAAGTGAAT CGCTTTGTGT GCATTTGCCC TCCAGGTTTC 
ACTGGATCAG TGTGCCAGAT TGATATAGAC GACTGCTCTA GCACTCCATG TCAGAATGGT 
GCCAAATGCG TTGATCATCC ATCTGGCTAT GACTGTATAT GTGCAACAGG CTTCACAGGT 
GTTTTGTGCG AAGAAAATAT CAACAACTGT GAACCACCAC CCTGTCATTA TGGAACATGC 
CAAGATGGCA TTGATTCCTA TACGTGCATT TGTGAGCCTG GCTATATGGG TGCCATTTGC 
AGCGACCAGA TCGATGAATG CCTGAGTAAC CCTTGTCTCA ATGAAGGCCG CTGTGTTGAC 
CTTGTCAATG GCTATCAGTG TAACTGCCTA CCTGGAACAT CTGGGCCAAG ATGTGAGAAG 
AATGTTGATG ACTGTGCAAG CAACCCGTGC ACCTATGGGA ACTGTGTGGA TGGGATAAAC 
CGCTACGACT GTGTCTGCTC CCCAGGATTT ACAGGCCCTC AGTGTAAAGA TGACATTGAT 
GAGTGTGCCT CAAACCCCTG CCATAATGGA GGAACGTGCG TAAATGAACA GAACAGATTT 
CGCTGTATCT GTCCGGAGGG ATTTCGCCAG CCCAGCTGTT TTTCGCAGGT GGACGAATGT 
GTCAATAACC CATGTATCCA CGGCAACTGC ACAGATGACG TCAATGGGTA CAAGTGTCTC 
TGCCAACCAG GCTGGACAGG CAAGAAATGC GAGGTGGATA AAAACGAGTG TCTTTCCAAC 
CCCTGTCAGA ATGGGGGGAC ATGCGATAAC CTGGTGAATG GATACAGATG CTCCTGCAAG 
AAAGGGTTCA AGGGTTACAA CTGTCAGATT GACATCGATG AATGTGCTTC AAATCCATGT 
TTAAACCATG GGACGTGCAG AGATGGGATC AATGGTTACA CGTGCCACTG CGGGCTGCCG 
TATACTGGTA CAAACTGCCA GAACATTCTG ACCCCCTGTA GCCCTGAACC GTGCCACAAT 
GGTGGAATTT GTCAGGAATC TGAAGATTTC AAGAGTTTCA GCTGTCTGTG TGCAACTGGC 
TGGCACGGTC AGAGGTGTAC TATTGATATT GATGAATGTA TAACCAACCC CTGCAAAAAC 
TATGGTCGCT GCCAAAATAC CAAAGGCGGA TACATTTGCC ATTGCTTTGA AGGCTTTAAT 
GGAGTCAACT GTGAAAACAA CATTGACGAT TGCCTTTCTA ATCCGTGTCA GAATGGGGCT 
TCCTGTGTAG ATGGAATCAA CACTTTCTCT TGTAACTGCC TGGCGGGGTT CCATGGGGAC 
AAGTGCCAGA AAGATGTGGA TGAATGTGCA AGCAACCCTT GTAAAAATGG AGGCTCCTGC 
ACAGACTACG TCAACAGCTA CACGTGCAAG TGTCAGCCGG GATTTGATGG AATCCACTGT 
GATAACAACA TTGATGAATG CACAGACACG TCCTGCTTCA ATGGTGCTAC CTGCGTGGAT 
GGCATTAATT CCTTCACCTG CCAGTGCCCT CAGGGATTTA CAGGACAATT TTGTCTGTTT 
GAGATCAATG AGTGTGGGTC CCACCCCTGC ATTAATGGAG GCACTTGTGT GGATGGAATG 
GGAACATATC ACTGCACTTG TCCTGTCGGA TACAGAGGCA AGAACTGTGA GGAGTTGGTT 
GATCTGTGCA GTGGCTTTCC ATGTAAAAAC AGAGGCATCT GCAAGCAAGT GAAAACAGAA 
CCCAAATGCA TTTGTCCAAC GGGTTGGACT GGCATTTACT GTGATATCCC AGATGTCTCT 
TGTGAAGTGG CTGCGTCTCA GAGGGGTGTG GCAGTGCAAG ATCTATGTCA GCATGCTGGC 
ATTTGCATCA ATACTGGCTT CAGTCACCGT TGCCAGTGCC GACAAGGCTA CATCGGCAGT 
TACTGTGAGG GGGAACTGGA TGAGTGTGCC TCCAATCCAT GCCAGAATAG CGCTACTTGT 
GTGGATCGTC ATGGGGGATA CGAGTGCAAG TGCTTGCCAG GGTACCAAGG AGTAAACTGC 
GAGTATGAGA TAGACGAGTG CCAGATCCAG CCTTGCCACA ATGGTGGGAC ATGTGTTGAC 
TTGGTAAATC AATTCCGATG CTCCTGCCCT CCAGGAACAC GAGGTCCCCT CTGTGAAGAA 
AATATAGATG ACTGTGCTCC AAGTTCTGAA GGGCCGCACT GTTTCAATGG TGGAAGGTGC 
TTAGATAAAA TTGGCGGCTA CAGCTGTGAA TGCCTCCCTG GGTTTGCAGG GGAGAGGTGT 
GAAGGCGATG TGAATGAATG CTTGTCCAAT CCTTGTAGTG CAAAGGGCAG CCTGGACTGC 
ATTCAGCTGG ATAACAACTA TCTTTGCAAG TGCAAGCCTG GATTCACAGG TCGCCACTGT 
GAGACGATAC AAGATTTGTG CCCAACTCGA CCTTGTCTGA ATGGTGGGAC ATGCGCTGTT 
GCTGTGAATA TGCCTGAAGG ATTTACCTGT CAGTGTCCAC CAGGCTTTTC TGGTTCTAAA 
TGCCAGTACA GTAATAATAC ATGTGGACAC TATAAGTGCA AAGTTGGGGA AAACTGTATT 
AAAACTCCTT TGGGACCAAG GTGCTACTGT CCCACGGGAC CAGCTGGGAA TGCATGCCAA 
TCTAAATTAG GCTGTGCCAG CCAGCCCTGT AAAAACAATG GAGTTTGCCA GAGAAAGGAT 
CAGCCTCCCT ATTATACATG CAATTGCCCT AATGGAATTG TTGGACTCCA GTGTGAAATT 
CTATTGATTG ATGGGTGTCA CCTCCACCAC TGTCCTGATA AAGCAAGAGA CGGGTACTGC 
GATCAGGAGT GTAACACGCA TGAATGCCTT TGGGATGGTG GAGACTGCTC TTTAACAATG 
GAAGACCCAT GGGCCAACTG TTCCTCCTCA TTGAAATGCT GGGAATATTT TAATGGCCAA 
TGTGATGAGC TGTGTAATAC ACCTGAGTGC CTGTTTGACA ACTTTGAGTG TCAAGAAAAA 
GAGTCAATCT GCAAGTATGA TAAGTACTGT GAAGATCACT ATGCAAATGG TCACTGTGAC 
AAAAGCTGTA ACACAAAAGA ATGTGGCTGG GATGGACTCG ACTGCTCTAC AGACAAACCT 
GAGAATCTTG CTGAGGGTAC CTTGGTCATA GTAGTGCTAA TGCCCCCAAA GGAACTGCTA 
AAAAATTCCA GAAATTTCCT CCGGGAGTTA GGCAGCATTC TTCGCACCAA CCTAAGGATC 
AAAAGGAATC CAGATGGGAG CCCCATGGTC TTTCCATATT TTAGAGAAAA ATCAAGCGCA 
CAGCGTTCAC GTAGATCTCT CTTAGAATTA CGCGTAGTCC GGGAGCTTGA GGAGCAGCCA 
ATTGGCTCAA AAGTCTCATT AGAAATTGAC AACCAACAGT GTGTCCTGGA TTCAGATCAG 
TGCTTTAAGA CTACAGACTC CGCTGCTGCC ATGCTGGCTG CCCATGCTGT ACAGGGTACA 
CTTAATTATC CCCTGGTCTC AGTGGGGGTT GAAAAGGCGC CACCATCGTC CCAGACACTG 
ATGTACATAT TGCCCGTTAT ATTGGCCATC ATTATTGTCA TTCTGCTGCT GGTTGTGGTA 
CTGGCCAAAA AGAAGCGCAA ACATGGCACC CTTTGGTTCC CTGAAGGTTT CCTCCTGCAC 
CGGGACACCA GCAACCAGAA GCGCAGAGAG CCTGTTGGGC AGGATGCTGT TGGAATGAAA 
AATTTGTCTG TGCCCATAGT GGATGGAAAC TTACTGGATT CTAATCAGAA TGATCACTGG 
TCAGATGATC GAGGACCACA GCCAAAGAAA TCCAAGTCTG AAGGTCAGGC TCTCCTGTCG 
GGAGGAGATG ACCAAGTTGA TAGCCGTCAG TGGACACAGC AGCACCTGGT GGCTGCAGAT 
ATTCGGATGA CCCCATCATT GGCTCTCACA CCTCCTCAAG CAGAGCAAGA CATGGATGTC 
ATAGATGTCA ACGTGCGAGG GCCAGATGGC TGCACACCAC TTATGTTGGC CTCCCTGCGA 
ATGGGAAGCC CTCACATAGA GGAAGATGAT GAGGAGGCAG AAGATCCATC TGCTAACGTC 
ATTACTGACC TGATTTATCA GGGAGCTTGT TTGCAGGCCC AGACAGATCG CACAGGAGAA 
ACTGCCCTGC ACCTGGCAGC TCGCTACTCA AGGGCCGATG CTGCCAAGCG ATTGCTGGAT 
GCTGGAGCTG ATGCCAACTC TCAGGACAGT ATGGGGCGGA CCCCTCTACA TGCAGCGGTC 
TCTGCTGATG CACAGGGGGT TTTCCAGATT TTGATCCGTA ACAGAGTCAC AGACCTCGAT 
GCGAGGATGA ATGATGGTAC AACCCCTCTC ATCTTAGCTG CCCGCCTTGC TGTGGAAGGG 
ATGGTTGCAG ACTTAATAAA CTGCCAGGCT GATGTTAATG CAGTGGATGA TCATGGTAAA 
TCTGCACTTC ACTGGGCTGC TGCAGTAAAC AATGTTGAGG CTACAATTGT TCTGCTAAAG 
AATGGAGCAA ATAGGGATAT GCAAGACAAC AAGGAAGAAA CACCCTTGTT TCTTGCTGCT 
AGAGAGGGTA GTTTTGAAGC AGCCAAAATG CTTTTGGATT ACTTTGCAAA CCGAGAAATC 
ACTGACCATA TGGATCGTCT TCCCCGTGAC ATTGCCAAGG ACAGAATGCA TCATGACATT 
GTGCGCCTGT TGGACGAGTA TAACTTGGTG CACAGCCCAC AAGGACAGCC TGGTACAATG 
CTTTCAAATT CCCTGTCGCC AGCAGTGTGT GGCTCCAACA GATCTTTCCT GAACCTGAAA 
CATGCCGCAC AAACAAAGAA GGCCAGAAGG CCAAGTGCCA AAAGTGTAGC ACCTCGAAGT 
CTTCCAAACT TAGCCAAAGA ACCAAAAGAT ACCAAAAACA GGAGGAGGAA AAAGTCAGAT 
ACTGACAGTA AAGCGCAGCT TTCTGAAAGC TCAGTAACAT TATCCCCTGT GGATTCTCTT 
GAATCCCCCC ATGCTACTTC ATCTCCTACC ATTACATCTC CTGGAGCGCT GCATACGTCC 
CCTGGACAGA TGATTGCAGC CTCTACGTTG CAGCCACCAC GCACAACAAT GTCTTTAACT 
AATCTTCAGC CCGTTGGTTG TGCAGCCAAT AATATGCTGC CTTCTGTTGG ACAGTTGTTA 
TCACACTCGG CTCAAAATAA TGTTCTTACA GCACAAAGAC AGCTCACTAT GTCATCTGAT 
TGGCTGAGCC GCATGGAAAG GAATGACACC CAGTATAATG AAATGTTTGG TATAGTCCAC 
CATGACACAA ATAACCACTC ATGTATTCCT CAACAAAATG GTATTATCCA AGCAGATATC 
AAGCCAACAC TGCATATGGG TGTGGCTCGG GATGCTTTGC CTCCTATTAT GACTTTCCAG 
CTTATCCCAA AGAGTGGAAT GAACCAGCAG TCTGGCATCC AGACACTTGT GCAGCAGGCT 
AGTCATGGTC AGAATGTGGC TGCCATGTAT CAGATTCCTG ATAGAGCACG GATCACTGGC 
TCTTCTGTTC CTATAGCAAT GATGCATCAA CATGATGGAC AGTTATCTCA AACTATGCTC 
ACAGCTTACC ATCCCCTTCA GAACCCAATG GGAAAATATC CAACCCCACC ATCACAACAC 
AGCTACACTT CCACTGATAA AACCCCAAAC CATAATGGAC ATTTACCCGG AGAGCACCCT 
TACTTGACCC CTTCACCAGA ATCTCCAGAT CAGTGGTCCA GCTCCTCACC TAACTCTGCC 
TCTGATTGGT CAGATGTAAC TACAAGTCCG ACCCCTAATG GCAATCAAAG AATGCCTGCC 
ACACATATGC CTGAACAATC ACACAATGGC ATGCAGATTT ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939126.3
CDS	494..7861
Translation	MGAQYVLGLCVLLLGMPGCDGLQCRGASEPCLNEGKCITNDDGSAYCKCTDAFVGKYCQLQNPCQETICLNGGTCKVSSDLSKGVCTCAPGFGGENCKQPVANVCSLTQPCMNGGTCHLLTLELYDCICPFGWRGNDCKFIDACASQPCANGSTCIPNENQYTCSCQAGYTGLKCEVDVNECGPPGMCKNGGTCENFPGSYRCQCHPGYTGQRCESPFIPCSPSPCMNGGTCRQTSEFAYECNCLPGFDGKNCETNIDDCPSHKCQNGGTCVDGVNTYNCRCPPQWTGQFCTEDVDECQLQPNACQNGGTCTNSNGGYNCVCVNGWSGDDCSQNIDDCATAACANGSTCIDRVASFICVCPEGKIGLLCHKNDACFSNPCHMGSLCDTNPLTGQAICTCPSGYKGTACTEDIDECSLVNSNPCEHAGKCVNTEGSFYCECSKGFTGPRCEMDINECLSEPCQNDATCLDKIGGFTCLCMPGYKGIHCEIEINECLSNPCVNNGECVDKVNRFVCICPPGFTGSVCQIDIDDCSSTPCQNGAKCVDHPSGYDCICATGFTGVLCEENINNCEPPPCHYGTCQDGIDSYTCICEPGYMGAICSDQIDECLSNPCLNEGRCVDLVNGYQCNCLPGTSGPRCEKNVDDCASNPCTYGNCVDGINRYDCVCSPGFTGPQCKDDIDECASNPCHNGGTCVNEQNRFRCICPEGFRQPSCFSQVDECVNNPCIHGNCTDDVNGYKCLCQPGWTGKKCEVDKNECLSNPCQNGGTCDNLVNGYRCSCKKGFKGYNCQIDIDECASNPCLNHGTCRDGINGYTCHCGLPYTGTNCQNILTPCSPEPCHNGGICQESEDFKSFSCLCATGWHGQRCTIDIDECITNPCKNYGRCQNTKGGYICHCFEGFNGVNCENNIDDCLSNPCQNGASCVDGINTFSCNCLAGFHGDKCQKDVDECASNPCKNGGSCTDYVNSYTCKCQPGFDGIHCDNNIDECTDTSCFNGATCVDGINSFTCQCPQGFTGQFCLFEINECGSHPCINGGTCVDGMGTYHCTCPVGYRGKNCEELVDLCSGFPCKNRGICKQVKTEPKCICPTGWTGIYCDIPDVSCEVAASQRGVAVQDLCQHAGICINTGFSHRCQCRQGYIGSYCEGELDECASNPCQNSATCVDRHGGYECKCLPGYQGVNCEYEIDECQIQPCHNGGTCVDLVNQFRCSCPPGTRGPLCEENIDDCAPSSEGPHCFNGGRCLDKIGGYSCECLPGFAGERCEGDVNECLSNPCSAKGSLDCIQLDNNYLCKCKPGFTGRHCETIQDLCPTRPCLNGGTCAVAVNMPEGFTCQCPPGFSGSKCQYSNNTCGHYKCKVGENCIKTPLGPRCYCPTGPAGNACQSKLGCASQPCKNNGVCQRKDQPPYYTCNCPNGIVGLQCEILLIDGCHLHHCPDKARDGYCDQECNTHECLWDGGDCSLTMEDPWANCSSSLKCWEYFNGQCDELCNTPECLFDNFECQEKESICKYDKYCEDHYANGHCDKSCNTKECGWDGLDCSTDKPENLAEGTLVIVVLMPPKELLKNSRNFLRELGSILRTNLRIKRNPDGSPMVFPYFREKSSAQRSRRSLLELRVVRELEEQPIGSKVSLEIDNQQCVLDSDQCFKTTDSAAAMLAAHAVQGTLNYPLVSVGVEKAPPSSQTLMYILPVILAIIIVILLLVVVLAKKKRKHGTLWFPEGFLLHRDTSNQKRREPVGQDAVGMKNLSVPIVDGNLLDSNQNDHWSDDRGPQPKKSKSEGQALLSGGDDQVDSRQWTQQHLVAADIRMTPSLALTPPQAEQDMDVIDVNVRGPDGCTPLMLASLRMGSPHIEEDDEEAEDPSANVITDLIYQGACLQAQTDRTGETALHLAARYSRADAAKRLLDAGADANSQDSMGRTPLHAAVSADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVADLINCQADVNAVDDHGKSALHWAAAVNNVEATIVLLKNGANRDMQDNKEETPLFLAAREGSFEAAKMLLDYFANREITDHMDRLPRDIAKDRMHHDIVRLLDEYNLVHSPQGQPGTMLSNSLSPAVCGSNRSFLNLKHAAQTKKARRPSAKSVAPRSLPNLAKEPKDTKNRRRKKSDTDSKAQLSESSVTLSPVDSLESPHATSSPTITSPGALHTSPGQMIAASTLQPPRTTMSLTNLQPVGCAANNMLPSVGQLLSHSAQNNVLTAQRQLTMSSDWLSRMERNDTQYNEMFGIVHHDTNNHSCIPQQNGIIQADIKPTLHMGVARDALPPIMTFQLIPKSGMNQQSGIQTLVQQASHGQNVAAMYQIPDRARITGSSVPIAMMHQHDGQLSQTMLTAYHPLQNPMGKYPTPPSQHSYTSTDKTPNHNGHLPGEHPYLTPSPESPDQWSSSSPNSASDWSDVTTSPTPNGNQRMPATHMPEQSHNGMQIYA

Target ORF information:

RefSeq Version	XM_002939080.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis notch 2 (notch2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939080.5

ORF Insert Sequence:

ATGGGGGCGC AGTATGTGCT GGGGCTGTGT GTCCTGCTGC TGGGGATGCC GGGCTGTGAC 
GGCTTACAGT GTCGAGGTGC CAGTGAGCCA TGCTTAAATG AAGGAAAATG TATCACCAAT 
GATGACGGCT CTGCATACTG CAAATGCACA GATGCATTTG TTGGAAAATA CTGCCAACTG 
CAAAACCCTT GCCAAGAAAC CATATGCCTC AATGGCGGCA CCTGTAAAGT CAGCTCTGAC 
CTGTCTAAAG GTGTGTGTAC TTGCGCCCCA GGATTCGGCG GGGAGAACTG CAAGCAGCCA 
GTTGCCAATG TCTGCTCCCT TACTCAGCCT TGTATGAACG GGGGCACCTG CCACCTGCTT 
ACTCTGGAAC TCTATGACTG TATCTGCCCC TTTGGATGGA GAGGAAATGA TTGCAAATTC 
ATTGATGCCT GTGCCTCTCA GCCATGCGCC AACGGCAGTA CCTGCATCCC CAATGAGAAC 
CAGTATACCT GTTCTTGTCA GGCTGGATAC ACCGGCCTCA AGTGTGAAGT GGATGTGAAC 
GAGTGTGGCC CGCCAGGGAT GTGCAAAAAT GGAGGAACTT GTGAGAATTT TCCTGGGTCC 
TACAGGTGTC AGTGTCACCC GGGTTACACT GGTCAGCGCT GTGAGAGCCC CTTCATCCCC 
TGCTCTCCTT CCCCATGTAT GAATGGTGGC ACCTGCCGTC AGACTAGTGA ATTTGCCTAT 
GAATGCAACT GCCTGCCAGG ATTTGATGGG AAAAACTGTG AAACCAACAT AGATGATTGT 
CCCAGTCATA AGTGCCAGAA TGGTGGAACC TGTGTGGATG GGGTGAACAC CTACAACTGC 
AGGTGTCCTC CCCAGTGGAC TGGCCAGTTT TGCACAGAGG ATGTTGATGA GTGCCAACTC 
CAGCCCAATG CTTGCCAAAA TGGTGGGACT TGTACCAACA GCAATGGAGG CTACAACTGC 
GTCTGTGTAA ATGGATGGAG TGGGGATGAT TGCAGCCAGA ATATCGATGA CTGTGCCACT 
GCTGCCTGTG CCAATGGGTC TACTTGTATT GATCGAGTTG CTTCCTTTAT ATGTGTTTGC 
CCAGAAGGAA AGATTGGACT CCTGTGTCAC AAAAATGATG CCTGCTTTAG TAATCCTTGC 
CACATGGGCT CTTTGTGTGA TACCAACCCT CTGACTGGAC AAGCCATTTG CACTTGCCCC 
TCTGGGTACA AAGGGACTGC CTGCACAGAG GACATAGATG AATGCTCTTT GGTTAACAGC 
AATCCCTGTG AGCATGCAGG AAAATGTGTC AATACTGAAG GTTCCTTTTA TTGTGAATGC 
TCCAAAGGAT TCACCGGGCC CCGCTGTGAA ATGGACATTA ATGAGTGCCT ATCTGAGCCA 
TGCCAGAATG ATGCCACCTG TCTGGATAAA ATCGGAGGCT TCACATGTTT GTGTATGCCA 
GGTTACAAGG GAATACACTG TGAAATTGAG ATCAATGAAT GCCTAAGCAA CCCCTGTGTG 
AACAATGGAG AGTGTGTTGA CAAAGTGAAT CGCTTTGTGT GCATTTGCCC TCCAGGTTTC 
ACTGGATCAG TGTGCCAGAT TGATATAGAC GACTGCTCTA GCACTCCATG TCAGAATGGT 
GCCAAATGCG TTGATCATCC ATCTGGCTAT GACTGTATAT GTGCAACAGG CTTCACAGGT 
GTTTTGTGCG AAGAAAATAT CAACAACTGT GAACCACCAC CCTGTCATTA TGGAACATGC 
CAAGATGGCA TTGATTCCTA TACGTGCATT TGTGAGCCTG GCTATATGGG TGCCATTTGC 
AGCGACCAGA TCGATGAATG CCTGAGTAAC CCTTGTCTCA ATGAAGGCCG CTGTGTTGAC 
CTTGTCAATG GCTATCAGTG TAACTGCCTA CCTGGAACAT CTGGGCCAAG ATGTGAGAAG 
AATGTTGATG ACTGTGCAAG CAACCCGTGC ACCTATGGGA ACTGTGTGGA TGGGATAAAC 
CGCTACGACT GTGTCTGCTC CCCAGGATTT ACAGGCCCTC AGTGTAAAGA TGACATTGAT 
GAGTGTGCCT CAAACCCCTG CCATAATGGA GGAACGTGCG TAAATGAACA GAACAGATTT 
CGCTGTATCT GTCCGGAGGG ATTTCGCCAG CCCAGCTGTT TTTCGCAGGT GGACGAATGT 
GTCAATAACC CATGTATCCA CGGCAACTGC ACAGATGACG TCAATGGGTA CAAGTGTCTC 
TGCCAACCAG GCTGGACAGG CAAGAAATGC GAGGTGGATA AAAACGAGTG TCTTTCCAAC 
CCCTGTCAGA ATGGGGGGAC ATGCGATAAC CTGGTGAATG GATACAGATG CTCCTGCAAG 
AAAGGGTTCA AGGGTTACAA CTGTCAGATT GACATCGATG AATGTGCTTC AAATCCATGT 
TTAAACCATG GGACGTGCAG AGATGGGATC AATGGTTACA CGTGCCACTG CGGGCTGCCG 
TATACTGGTA CAAACTGCCA GAACATTCTG ACCCCCTGTA GCCCTGAACC GTGCCACAAT 
GGTGGAATTT GTCAGGAATC TGAAGATTTC AAGAGTTTCA GCTGTCTGTG TGCAACTGGC 
TGGCACGGTC AGAGGTGTAC TATTGATATT GATGAATGTA TAACCAACCC CTGCAAAAAC 
TATGGTCGCT GCCAAAATAC CAAAGGCGGA TACATTTGCC ATTGCTTTGA AGGCTTTAAT 
GGAGTCAACT GTGAAAACAA CATTGACGAT TGCCTTTCTA ATCCGTGTCA GAATGGGGCT 
TCCTGTGTAG ATGGAATCAA CACTTTCTCT TGTAACTGCC TGGCGGGGTT CCATGGGGAC 
AAGTGCCAGA AAGATGTGGA TGAATGTGCA AGCAACCCTT GTAAAAATGG AGGCTCCTGC 
ACAGACTACG TCAACAGCTA CACGTGCAAG TGTCAGCCGG GATTTGATGG AATCCACTGT 
GATAACAACA TTGATGAATG CACAGACACG TCCTGCTTCA ATGGTGCTAC CTGCGTGGAT 
GGCATTAATT CCTTCACCTG CCAGTGCCCT CAGGGATTTA CAGGACAATT TTGTCTGTTT 
GAGATCAATG AGTGTGGGTC CCACCCCTGC ATTAATGGAG GCACTTGTGT GGATGGAATG 
GGAACATATC ACTGCACTTG TCCTGTCGGA TACAGAGGCA AGAACTGTGA GGAGTTGGTT 
GATCTGTGCA GTGGCTTTCC ATGTAAAAAC AGAGGCATCT GCAAGCAAGT GAAAACAGAA 
CCCAAATGCA TTTGTCCAAC GGGTTGGACT GGCATTTACT GTGATATCCC AGATGTCTCT 
TGTGAAGTGG CTGCGTCTCA GAGGGGTGTG GCAGTGCAAG ATCTATGTCA GCATGCTGGC 
ATTTGCATCA ATACTGGCTT CAGTCACCGT TGCCAGTGCC GACAAGGCTA CATCGGCAGT 
TACTGTGAGG GGGAACTGGA TGAGTGTGCC TCCAATCCAT GCCAGAATAG CGCTACTTGT 
GTGGATCGTC ATGGGGGATA CGAGTGCAAG TGCTTGCCAG GGTACCAAGG AGTAAACTGC 
GAGTATGAGA TAGACGAGTG CCAGATCCAG CCTTGCCACA ATGGTGGGAC ATGTGTTGAC 
TTGGTAAATC AATTCCGATG CTCCTGCCCT CCAGGAACAC GAGGTCCCCT CTGTGAAGAA 
AATATAGATG ACTGTGCTCC AAGTTCTGAA GGGCCGCACT GTTTCAATGG TGGAAGGTGC 
TTAGATAAAA TTGGCGGCTA CAGCTGTGAA TGCCTCCCTG GGTTTGCAGG GGAGAGGTGT 
GAAGGCGATG TGAATGAATG CTTGTCCAAT CCTTGTAGTG CAAAGGGCAG CCTGGACTGC 
ATTCAGCTGG ATAACAACTA TCTTTGCAAG TGCAAGCCTG GATTCACAGG TCGCCACTGT 
GAGACGATAC AAGATTTGTG CCCAACTCGA CCTTGTCTGA ATGGTGGGAC ATGCGCTGTT 
GCTGTGAATA TGCCTGAAGG ATTTACCTGT CAGTGTCCAC CAGGCTTTTC TGGTTCTAAA 
TGCCAGTACA GTAATAATAC ATGTGGACAC TATAAGTGCA AAGTTGGGGA AAACTGTATT 
AAAACTCCTT TGGGACCAAG GTGCTACTGT CCCACGGGAC CAGCTGGGAA TGCATGCCAA 
TCTAAATTAG GCTGTGCCAG CCAGCCCTGT AAAAACAATG GAGTTTGCCA GAGAAAGGAT 
CAGCCTCCCT ATTATACATG CAATTGCCCT AATGGAATTG TTGGACTCCA GTGTGAAATT 
CTATTGATTG ATGGGTGTCA CCTCCACCAC TGTCCTGATA AAGCAAGAGA CGGGTACTGC 
GATCAGGAGT GTAACACGCA TGAATGCCTT TGGGATGGTG GAGACTGCTC TTTAACAATG 
GAAGACCCAT GGGCCAACTG TTCCTCCTCA TTGAAATGCT GGGAATATTT TAATGGCCAA 
TGTGATGAGC TGTGTAATAC ACCTGAGTGC CTGTTTGACA ACTTTGAGTG TCAAGAAAAA 
GAGTCAATCT GCAAGTATGA TAAGTACTGT GAAGATCACT ATGCAAATGG TCACTGTGAC 
AAAAGCTGTA ACACAAAAGA ATGTGGCTGG GATGGACTCG ACTGCTCTAC AGACAAACCT 
GAGAATCTTG CTGAGGGTAC CTTGGTCATA GTAGTGCTAA TGCCCCCAAA GGAACTGCTA 
AAAAATTCCA GAAATTTCCT CCGGGAGTTA GGCAGCATTC TTCGCACCAA CCTAAGGATC 
AAAAGGAATC CAGATGGGAG CCCCATGGTC TTTCCATATT TTAGAGAAAA ATCAAGCGCA 
CAGCGTTCAC GTAGATCTCT CTTAGAATTA CGCGTAGTCC GGGAGCTTGA GGAGCAGCCA 
ATTGGCTCAA AAGTCTCATT AGAAATTGAC AACCAACAGT GTGTCCTGGA TTCAGATCAG 
TGCTTTAAGA CTACAGACTC CGCTGCTGCC ATGCTGGCTG CCCATGCTGT ACAGGGTACA 
CTTAATTATC CCCTGGTCTC AGTGGGGGTT GAAAAGGCGC CACCATCGTC CCAGACACTG 
ATGTACATAT TGCCCGTTAT ATTGGCCATC ATTATTGTCA TTCTGCTGCT GGTTGTGGTA 
CTGGCCAAAA AGAAGCGCAA ACATGGCACC CTTTGGTTCC CTGAAGGTTT CCTCCTGCAC 
CGGGACACCA GCAACCAGAA GCGCAGAGAG CCTGTTGGGC AGGATGCTGT TGGAATGAAA 
AATTTGTCTG TGCCCATAGT GGATGGAAAC TTACTGGATT CTAATCAGAA TGATCACTGG 
TCAGATGATC GAGGACCACA GCCAAAGAAA TCCAAGTCTG AAGGTCAGGC TCTCCTGTCG 
GGAGGAGATG ACCAAGTTGA TAGCCGTCAG TGGACACAGC AGCACCTGGT GGCTGCAGAT 
ATTCGGATGA CCCCATCATT GGCTCTCACA CCTCCTCAAG CAGAGCAAGA CATGGATGTC 
ATAGATGTCA ACGTGCGAGG GCCAGATGGC TGCACACCAC TTATGTTGGC CTCCCTGCGA 
ATGGGAAGCC CTCACATAGA GGAAGATGAT GAGGAGGCAG AAGATCCATC TGCTAACGTC 
ATTACTGACC TGATTTATCA GGGAGCTTGT TTGCAGGCCC AGACAGATCG CACAGGAGAA 
ACTGCCCTGC ACCTGGCAGC TCGCTACTCA AGGGCCGATG CTGCCAAGCG ATTGCTGGAT 
GCTGGAGCTG ATGCCAACTC TCAGGACAGT ATGGGGCGGA CCCCTCTACA TGCAGCGGTC 
TCTGCTGATG CACAGGGGGT TTTCCAGATT TTGATCCGTA ACAGAGTCAC AGACCTCGAT 
GCGAGGATGA ATGATGGTAC AACCCCTCTC ATCTTAGCTG CCCGCCTTGC TGTGGAAGGG 
ATGGTTGCAG ACTTAATAAA CTGCCAGGCT GATGTTAATG CAGTGGATGA TCATGGTAAA 
TCTGCACTTC ACTGGGCTGC TGCAGTAAAC AATGTTGAGG CTACAATTGT TCTGCTAAAG 
AATGGAGCAA ATAGGGATAT GCAAGACAAC AAGGAAGAAA CACCCTTGTT TCTTGCTGCT 
AGAGAGGGTA GTTTTGAAGC AGCCAAAATG CTTTTGGATT ACTTTGCAAA CCGAGAAATC 
ACTGACCATA TGGATCGTCT TCCCCGTGAC ATTGCCAAGG ACAGAATGCA TCATGACATT 
GTGCGCCTGT TGGACGAGTA TAACTTGGTG CACAGCCCAC AAGGACAGCC TGGTACAATG 
CTTTCAAATT CCCTGTCGCC AGCAGTGTGT GGCTCCAACA GATCTTTCCT GAACCTGAAA 
CATGCCGCAC AAACAAAGAA GGCCAGAAGG CCAAGTGCCA AAAGTGTAGC ACCTCGAAGT 
CTTCCAAACT TAGCCAAAGA ACCAAAAGAT ACCAAAAACA GGAGGAGGAA AAAGTCAGAT 
ACTGACAGTA AAGCGCAGCT TTCTGAAAGC TCAGTAACAT TATCCCCTGT GGATTCTCTT 
GAATCCCCCC ATGCTACTTC ATCTCCTACC ATTACATCTC CTGGAGCGCT GCATACGTCC 
CCTGGACAGA TGATTGCAGC CTCTACGTTG CAGCCACCAC GCACAACAAT GTCTTTAACT 
AATCTTCAGC CCGTTGGTTG TGCAGCCAAT AATATGCTGC CTTCTGTTGG ACAGTTGTTA 
TCACACTCGG CTCAAAATAA TGTTCTTACA GCACAAAGAC AGCTCACTAT GTCATCTGAT 
TGGCTGAGCC GCATGGAAAG GAATGACACC CAGTATAATG AAATGTTTGG TATAGTCCAC 
CATGACACAA ATAACCACTC ATGTATTCCT CAACAAAATG GTATTATCCA AGCAGATATC 
AAGCCAACAC TGCATATGGG TGTGGCTCGG GATGCTTTGC CTCCTATTAT GACTTTCCAG 
CTTATCCCAA AGAGTGGAAT GAACCAGCAG TCTGGCATCC AGACACTTGT GCAGCAGGCT 
AGTCATGGTC AGAATGTGGC TGCCATGTAT CAGATTCCTG ATAGAGCACG GATCACTGGC 
TCTTCTGTTC CTATAGCAAT GATGCATCAA CATGATGGAC AGTTATCTCA AACTATGCTC 
ACAGCTTACC ATCCCCTTCA GAACCCAATG GGAAAATATC CAACCCCACC ATCACAACAC 
AGCTACACTT CCACTGATAA AACCCCAAAC CATAATGGAC ATTTACCCGG AGAGCACCCT 
TACTTGACCC CTTCACCAGA ATCTCCAGAT CAGTGGTCCA GCTCCTCACC TAACTCTGCC 
TCTGATTGGT CAGATGTAAC TACAAGTCCG ACCCCTAATG GCAATCAAAG AATGCCTGCC 
ACACATATGC CTGAACAATC ACACAATGGC ATGCAGATTT ATGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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notch2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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