notch2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	notch2
Entrez Gene ID	58065
Full Name	notch 2
Synonyms	notch6,sb:cb884
General protein information	Preferred Names notch 2 Names neurogenic locus notch homolog protein 2 notch homolog 2 notch receptor protein 6
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001115094.2	NP_001108566.2	neurogenic locus notch homolog protein 2 precursor

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	NOTCH2	XP_001114248.1
Rattus norvegicus (Norway rat)	Notch2	NP_077334.1
Homo sapiens (human)	NOTCH2	NP_077719.2
Canis lupus familiaris (dog)	NOTCH2	XP_005630745.1
Bos taurus (cattle)	NOTCH2	XP_002686160.2
Xenopus tropicalis (tropical clawed frog)	LOC100486344	XP_002939126.2
Pan troglodytes (chimpanzee)	NOTCH2	XP_513697.3
Mus musculus (house mouse)	Notch2	NP_035058.2
Gallus gallus (chicken)	NOTCH2	NP_001238962.1
Danio rerio (zebrafish)	notch2	NP_001108566.1

Gene Ontology
Bibliography

Related articles in PubMed

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Xue Y, Liu D, Cui G, Ding Y, Ai D, Gao S, Zhang Y, Suo S, Wang X, Lv P, Zhou C, Li Y, Chen X, Peng G, Jing N, Han JJ, Liu F
Cell reports27(5)1567-1578.e5(2019 04)

Deficiency of lrp4 in zebrafish and human LRP4 mutation induce aberrant activation of Jagged-Notch signaling in fin and limb development.
Tian J, Shao J, Liu C, Hou HY, Chou CW, Shboul M, Li GQ, El-Khateeb M, Samarah OQ, Kou Y, Chen YH, Chen MJ, Lyu Z, Chen WL, Chen YF, Sun YH, Liu YW
Cellular and molecular life sciences : CMLS76(1)163-178(2019 Jan)

Transcriptomics reveals complex kinetics of dorsal-ventral patterning gene expression in the mandibular arch.
Sharma PP, MacLean AL, Meinecke L, Clouthier DE, Nie Q, Schilling TF
Genesis (New York, N.Y. : 2000)57(1)e23275(2019 01)

Secretogranin-II plays a critical role in zebrafish neurovascular modeling.
Tao B, Hu H, Mitchell K, Chen J, Jia H, Zhu Z, Trudeau VL, Hu W
Journal of molecular cell biology10(5)388-401(2018 10)

Spine Patterning Is Guided by Segmentation of the Notochord Sheath.
Wopat S, Bagwell J, Sumigray KD, Dickson AL, Huitema LFA, Poss KD, Schulte-Merker S, Bagnat M
Cell reports22(8)2026-2038(2018 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following notch2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the notch2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98532	NM_001115094.2 Latest version!	Danio rerio notch receptor 2 (notch2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

notch2 ( NM_001115094.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001108566.2 Danio rerio notch receptor 2 (notch2), mRNA.

Price & Availability↑

CloneID	ODa98532
Clone ID Related Accession (Same CDS sequence)	NM_001115094.2
Accession Version	NM_001115094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570204800000
Organism	Danio rerio(zebrafish)
Product	neurogenic locus notch homolog protein 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH438786.1 and FP016062.4. On May 17, 2016 this sequence version replaced NM_001115094.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQH01003462.1, GFIL01028438.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGGCCAAT TACCCGGTGT CTCTTCGGGC GGAGTCATCC TTCTCCTTAT TTGCTTTCTC 
AAAACATCAT ATGCTCTGCA GTGTATCGAC CACGAGAAGC CCTGTGTGAA TGGTACCTGT 
GTCATCTCAA GCAACGGGAC ACACTACTGC AGGTGTGCTC CTGGTTTCTT AGGGGAGTAC 
TGTCATCACA GTGACCCTTG TAATCTGTTG TATTGTAAAA ATGGAGGAGA GTGCAAGGTG 
AGCGTGGCCG GTGTCCCTGG GAGCCCCTCA TGCGTGTGTC CTCTGGGCTT TGCGGGTGAG 
CACTGCGAAA TCCAGAAAAA CTCCACCTGC TACCCCAATA ACCCTTGCAC CAATGGAGGA 
AAGTGCTTGC TCCTTCCCAA CGACCAATAC AAATGCCAGT GTGCCCTTGG GTGGACAGGT 
CAGCATTGTG AGCAAGAGGA CGCATGTCTG TCCAGCCCAT GTGCCAACAA GGGTATATGC 
AATGCTCTGC CAAATCGCGA ATACTCTTGT ACCTGTGCGC CTGGTTTTCA TGGTTCCCGT 
TGTCTTAACG ACACAGACGA GTGTGTGGAC CCGTTAGTAT GTAAGAACAA AGGCGTTTGT 
GTGAACACCA TAGGCTCATA TCGATGCAAC TGCCAACCTG GCTTCACCGG ATTGGACTGC 
GGGAGTCCTT ACTTCCCCTG CTCTCCGTCC CCCTGCCAAA ATGGTGGGAC CTGCCGGCAA 
ACAACAGAAA CCACCTATGT ATGCCACTGC CTGCCAGGTT TTAATGGTAC AAACTGTGAG 
GTGAACATTG ATGACTGTCC AGATCACCGC TGTCAGAATG GAGCCACATG CATGGATGGA 
GTGAACACCT ACAACTGCCA GTGCCCACCT GAATGGACAG GTCAGTTTTG CACTGATGAT 
GTGGATGAAT GCAGACTGCA GCCCAACGCC TGTCAGAACG GAGGCACATG CAGTAACACA 
CGCAACGGCT ACAACTGTGT GTGTGTGAAC GGCTGGAGCG GCCTGGACTG CTCTGAAAAC 
ATTGATGACT GTGCAGCTGA GCCCTGCACC GCTGGCTCCA CTTGCATCGA CCGTGTGGCT 
TCCTTCGTTT GCAGCTGCCC TCCCGGCAAG ACTGGTTTGC TGTGTCACAT TGATGATGCC 
TGCATCAGTA ACCCTTGCAA GATGGGCGCT CATTGCGACA CAAACCCTGT CAATGGCAAA 
TTTAGCTGTA ACTGCCCTTC AGGATACAAG GGCAGCACAT GTGCAGAGGA TATCGACGAG 
TGTGTCATTG GGCCAAACCC ATGTGAGCAT GGAGGCTCAT GTAAGAACAC AGAGGGGTCC 
TTCACTTGTA ACTGCGCTCC AGGTTACACC GGCCCACGTT GTGAGCAGGA CATCAATGAA 
TGTGGCTCCA ACCCCTGCCA GAATGATGCC ACCTGCTTGG ATCAGATAGG AGATTACACC 
TGTATCTGTA TGCCAGGCTT TGATGGAACA CACTGTGAAA ATGACATTAA TGAGTGTCTC 
AGCTCACCTT GTCTTAATAA CGGTAGATGT CTAGACCAGG TCAGCCGATT CGTCTGCGAG 
TGTCCTCAGG GTTTCACTGG GGAAACGTGT CAAGTGGACA TCGATGAGTG TGCCAGCACT 
CCGTGCCATA ATGGTGCCAA GTGTCTTGAC CGTCCTAACG GTTACGAGTG TGAATGTGCT 
GAAGGTTTCA CAGGGCCTTT GTGTATGGAA AACATCAACG ACTGTGAACC CCAACCCTGT 
CATCATGGCA AATGTATAGA TGGAATTGCT ACCTTTCACT GCGATTGTCT CCCAGGTTAT 
ATGGGCCCCA TCTGCAGCGA GCAGATACGT GAGTGCCAGA GTGATCCCTG CCAAAACGGA 
GGTCGCTGTG TTGACATGGT TAACAGATAC CAATGCAACT GCCAGCCGGG TACCGCAGGT 
GAGAACTGTC AGATTAACTA TGATGACTGT GCCAGTAATC CCTGCCAGCA CGGGACCTGT 
GAGGATGGAA TCAATGAGTA CAAATGCGTG TGCGAACCTG GATACACAGG GGAACGATGC 
GCTGATGAGA TCAATGAGTG CAGTTCAAAC CCATGTCAGA GTGGGGGGAC GTGTGAGGAT 
AAAGTGAATG GATTTAAGTG CAAGTGTCCA GTTGGGACTT ATGGGCCCCT GTGTCTCTCA 
GGGGCAGACC GTTGTATCCG CATGCCCTGC CGGCATGGAG AGTGCATTGG GAAGCAGGAA 
GGGTATCTTT GTCAATGTGA GCCAGGCTGG GAGGGTACGA ACTGTGACCA AGAGAAGAAC 
GAGTGTCTGT CCAACCCGTG TCAGAATGGT GGAACCTGTA ATGACCGTCT GAATGATTAT 
ACCTGCGTAT GCGCCCGTGG GTTTGCAGGT TTCAACTGTG AGATCAACAT AAATGAATGT 
GAGTCAAACC CCTGCATGAA TCAGGGAACC TGTGTGGATG GAGTCAACAG CTACGTATGC 
CACTGCAAAC CACCTTACAC AGGGAAACAC TGTCTGGAGA AGTTGATCCC ATGTGCCTCT 
CAGCCCTGTC AGAGACGAGG AGTCTGTCAG CCATCGCTGG ATTACACCAG CTACACCTGC 
AAATGCCACA GCGGCTGGGA GGGAGCCCAA TGCACAGAGG ATAAAGACGA GTGTAAGAAG 
AGCCCTTGTC AGAATGGTGC TCGCTGTGTG AATATAGTTG GCAGCTACCG TTGCGAATGC 
CCGCCTGGAT ACAGCGGGGA CAACTGCCAG ACCAACATCG ATGACTGCAG CTCTAATCCA 
TGCCGTAACG GGGGTACATG TGTTGATAAA GTGGGCCGGT ACTTGTGCGA GTGCAGAGCT 
GGTTTTTATG GCGAGCGCTG TGAGGAAGAG GTGGACGAGT GCGCCAGCAA CCCCTGCTGG 
AATGGAGGAC ACTGCACAGA CTACGTGAAC AGCTACACAT GCCAATGCCC GCCAGGCTAT 
GACGGCATCA ATTGTGAACG TGATATCCCA GACTGCACTG AAACCTCTTG TCTGAACAAT 
GGGACTTGTG TGGATGGAAT TAACCATTTT TCCTGCCGTT GTCGGCAAGG TTTTTCGGGC 
CAGTATTGCC AGTTTGAACT GAACGAGTGT GACTCTCATC CCTGTAAAAA CGGTGGCACC 
TGCATAGACG GTCTGGGGAC TTTCCACTGC AACTGCCCAC TAATGTACAA TGGAAAAACC 
TGTGAGTCAA TGGTGAATTT GTGCAGTCAG ATTAAGTGTC AGAATGGAGG TACTTGCATC 
CAGAAAGAAA TCAATTGGAG ATGCAGCTGT CAGCCTGGCT GGACAGGCCT GTATTGTGAC 
ATCCCCAGCA TGTCCTGCCA CGCTGTTGCC CTCAACAAAG GTGTGGCGGA GAACATGGTC 
TGCAAGCACG CAGGTCATTG TAAGAACATA GGCAACACAC ATCAGTGCGA GTGTCAGAAG 
GGATATACGG GCAGTTACTG CGAGACTGAG ATAGATGAAT GTCTATCCAG CCCTTGCAGA 
AATGGAGGGA CATGTGTGGA CTACCAGGGT GGATATGACT GCAAGTGTAA AGCAGGTTTT 
CAGGGTGTGA ACTGTGAATA TAATGTTAAC GAGTGCCAGT CAAATCCCTG TCGCAATGGA 
GGAACCTGCA TAGATCTGAT CAACCATTTC TCCTGCTCTT GCCCTCCTGG AACCAAAGGT 
TTGCAGTGTG AAGTGGACGT GGATGAATGT GCCCCGGAAT TTGGCCCGCG CTGTAAGAAT 
GGCGGCCAGT GTGTGGATGG TGTGGGCCGG TACACCTGCA ACTGCCCTCC TGGTTTCGCT 
GGAGAGCATT GCGAGGGAGA TGTGAACGAG TGTCGTTCTG GACCATGCTA CTCACCTGGC 
ACCATTGACT GCGTGCCACT TATCAACAGC TATCAATGCC GTTGCCGTCT CGGTTATACT 
GGTCAACGTT GTGAGTCAAT GGTTGATCTG TGCCAGTCCA AGCCATGCCA CAACAATGGT 
AGATGCACTA CGAACTCCAG CTCTGTACAC GGATACATCT GTACATGCCC ACCTGGTTTC 
TCTGGCTTTA ACTGTGGCAA TTATGAGATG TTCCCATGTG CCAGTTTACG CTGTCTGAAT 
GGTGGACAGT GTGTGGAGAT AAAGAGCCGC CCTCAGTGCC ACTGCCCAGT GGGTTTTACT 
GGAACACACT GTGAGATGTT AACAAACCAT TGCGTGTGTC AAAATGGTGG CACCTGCATG 
CCAGATGAGT CCAACAAGTT TAGCTGCCGG TGTCCACCAG GGTTTAGCGG GGAGACCTGT 
CAGGAGACTC CACCTTCTTG CCCTTATCCG GATTGCGAAC AGCGGGCTGG AGACAAAGTA 
TGTGACCTGC GTTGTAAGAA CCCGGAGTGT GACTGGGATG GAAGTGACTG TACGCTCCAC 
TGGGACAAAC CCTGGAAGAA CTGCAAAGCC TCAGTGTCCT GCCCAGACTT ATTTCATAAC 
GGCCGCTGTG ACCCAGAGTG CAACAATGCA GGCTGCCTAT TCGACAGCTT TGAATGCGAC 
AAACATTCAT CGGTGGGAAT TTGCAAATAC GACAAATACT GTGCAGATCA TTACGCCAAT 
GGTCATTGCA ACCAGGGCTG CAACACGGAA GCATGTGGCT GGGATGGTCT GGACTGTGCC 
AGAGACACTC CTCCCAAGCT GGCAAACGGC ACACTGGTGA TTGTGGTTCT GCTGCAACCC 
AAAGAGCTGC TGGCTGACCT TAAGGGTTTC CTGCGTTCTC TGGGGACACT CCTGCACACC 
AACCTGCAAG TGAAGCTAGA TGAGCATAAT AAACTTATGG TGTACCCATT CTATAGAAAT 
GAGCAGAATG AAATTCAAAA TGAAATTGCC GCTATGAAGC AACGTGGAAA ACGCGAGCTG 
GAGAACGAGA TTATCGGATC GAGGGTTCAC CTGGAGATTG ACAATCGGCT GTGTACTCAG 
AACTCAGACG AATGCATCTC CAGTGCAGAC CAGGCTGCAG CCTTTATAGC TGCTGAGTAC 
CTGAAGTCAG ATCTGCCGTA CCCTATCTAC TCCATCACCA CCAAGCCACC TAAAGAAACT 
GGGTTTAATT TCCTGTACCT GGTTGCTGTG GCTGCTGCCA TCATCCTGCT CATTTTGGTG 
CTGGGTGTTT TGGCTGCCAA GCGCAAACGC AAGCACGGCA CTCTGTGGCT TCCTGATGGC 
TTTCTGCCAA ACAAAGAGAG CAAGCGGAGG GAGCCAGTGG GACAGGATGA CTTTGGCATG 
AAGAGCATGC AGAAGCCACA GGATGGAGGA TTATTGGATT GCAGTTCGAA CCATCACTGG 
TCAGAAGAAG ATCATCTTCC TAAAAAACCT AGAATGGAGG ATAAGCCATT GCTTCCTGTT 
GGAGTGGATG GAGTGGACAG GAGAGAGTGG ACCCTGCAGC ATCATAAAGC TGCTGACATC 
ACACTCACCC CCCCTCAGGC AGACAACGAC ATGGAGAGTC TGGACGTCAA TGTTAAAGGG 
CCAGATGGTT TCACTCCTCT CATGCTGGCA TCTCTAAGAA ATGGTAGCAG CCCTGACTGT 
GGCAGCATGC TAGGAGAGGA GGAGGAGGAA AGTGGTGCTG ACGAGCCTGG CACAAACGTC 
ATTACCGACT TAATTGGCCA GGGTGCGTCG CTCAATGCCC AGACTGATCG CACCGGGGAG 
ACTGCACTTC ACCTCGCTGC ACGATACGCT CGAGCAGATG CTGCCAAGAG ACTGCTGGAC 
GCTGGTGCTG ATGCCAATGC TCATGATAAC ATGGGCCGTA CACCTCTTCA TGCGGCCGTA 
GCAGCAGATG CTCAAGGAGT TTTCCAGATT CTCATCCGGA ACCGTGCCAC TGATCTTGAT 
GCCCGTATGA ATGACGGAAC AACACCTCTC ATCCTGTCAG CCAGACTGGC AGTGGAAGGC 
ATGGTGGAGG AACTGGTGCA CTGCCATGCA GACGTTAATG CTGTGGACGA TCATGGCAAG 
TCAGCTCTGC ACTGGGCTGC TGCTGTGAAT AATGTGGATG CCACTTTGGT GCTGCTCAAA 
AATGGAGCCA ACCGAGACAT GCAGGACAAC AAGGAGGAGA CGCCGTTGTT TCTGGCGGCT 
AGAGAAGGCA GCTTTGAGGC TGCTCAAATT CTGCTTGACC ATTACTCAAA CCGTGACATC 
ACCGATCACC TGGACCGCCT GCCGCGTGAC ACCGCACAAG AGCGAATGCA TCATGACATT 
GTGCGTCTGC TGGATGAGTA CAACCTGGTG CACAGCCCAC ACTCCGGGCC CAACCATGTA 
GGAAATGGTG GAGGGCACTC ATCGATGGTC TGTGGCACAA ACGGGACAGG ATACATCCTC 
GGCATGCGTC CTGGACCACA GGGCAAAAAG AACCGCAGAG CTGGAGGAAA AGCAAATGGT 
GTAGCAGCCG GGACAGCCAA AGATCTGAAA GAAATGAAAG CCAAGAGACG GAAGAAACCA 
ACCGGAGGAG AGGGGCCAAG TGTGGTGCCG GTGACCACAG CTGGAGGGAA TGTAAGCAGA 
GCAGCTGGTG GTCTCTCCGA GAGCTCAGTT ACCATGTCTC CTGTGGATTC CCTAGAGTCT 
CCACACTCAT ATGCCGGAGA GCCCGTGGCC ACCTCCAGCA CGGCCAATTC TCCACCTCTG 
CTTAGCAGCC AGTCAGCGAG GCCCATGCTG CCCCCGGTGA GTCACATGCT GGGCCAGCAG 
CAAAGCTGGG TGGGTTTGTC TAAACACAGC TACAATGGCC ACATGTTTAG CCTCATGCCA 
CACCACCAAA TGAGTGCAGG CCATAGTGGC ATGCAGCAGC ACCACAGCCC AGGCATGTCC 
GCCCCAATGA ACGTCACCAT GAGCCGTGAA CAACTCCCGC CAATTGTCAC CTTCCAGATG 
ATGCCGACCG GCAGCGGGCA GAGCCTCCTC AAGCAGCCAC AACAGGGGCA GATGCAGGCC 
CAAGGGCAAA ATCAGCCACA GGCTCAGCAT CTACACTGCA GCCAGGGTAT GATGTACCCC 
ATTCCTGACG TGAGCATGCA GCACAGCCTA TCTCACGCCC TTCAGCACCC TTCTCATGCT 
CACAACCACA CACTCCAGCA TGGGCTGCCT GATGGCCAAG CACGGCAGGT GGTGTCCTAT 
CAGCCACGTC AGAGCCCTGT GGATAAGTAC CCCACGCCAC CTTCCCAGCA CAGCTACGCC 
ACTGCCGGCT CTGAGGGAAC CACCCCTGGA CATCCAGCAC AAAAACCCAG CGAACACCCC 
TACCTCACCC CTTCACCAGA GTCACCCGAC CCCTGGTCCT CCTCGTCCCC ACACTCCAAC 
TCAGACTGGT CCGATGTCAC CACAAGCCCC ACACCTCTAG GAAACCCACA CACAATGCCA 
CCTTCCTGCC ACACACACAT TCCCGAACAG GCCCAGCTGC AGCCCCCATC ACAGGCCGCG 
CAGCCAACAC AGTCCCAGCA GTCCCAGCGC AGCAGTGTGT ACGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108566.2
CDS	256..7683
Translation	MGQLPGVSSGGVILLLICFLKTSYALQCIDHEKPCVNGTCVISSNGTHYCRCAPGFLGEYCHHSDPCNLLYCKNGGECKVSVAGVPGSPSCVCPLGFAGEHCEIQKNSTCYPNNPCTNGGKCLLLPNDQYKCQCALGWTGQHCEQEDACLSSPCANKGICNALPNREYSCTCAPGFHGSRCLNDTDECVDPLVCKNKGVCVNTIGSYRCNCQPGFTGLDCGSPYFPCSPSPCQNGGTCRQTTETTYVCHCLPGFNGTNCEVNIDDCPDHRCQNGATCMDGVNTYNCQCPPEWTGQFCTDDVDECRLQPNACQNGGTCSNTRNGYNCVCVNGWSGLDCSENIDDCAAEPCTAGSTCIDRVASFVCSCPPGKTGLLCHIDDACISNPCKMGAHCDTNPVNGKFSCNCPSGYKGSTCAEDIDECVIGPNPCEHGGSCKNTEGSFTCNCAPGYTGPRCEQDINECGSNPCQNDATCLDQIGDYTCICMPGFDGTHCENDINECLSSPCLNNGRCLDQVSRFVCECPQGFTGETCQVDIDECASTPCHNGAKCLDRPNGYECECAEGFTGPLCMENINDCEPQPCHHGKCIDGIATFHCDCLPGYMGPICSEQIRECQSDPCQNGGRCVDMVNRYQCNCQPGTAGENCQINYDDCASNPCQHGTCEDGINEYKCVCEPGYTGERCADEINECSSNPCQSGGTCEDKVNGFKCKCPVGTYGPLCLSGADRCIRMPCRHGECIGKQEGYLCQCEPGWEGTNCDQEKNECLSNPCQNGGTCNDRLNDYTCVCARGFAGFNCEININECESNPCMNQGTCVDGVNSYVCHCKPPYTGKHCLEKLIPCASQPCQRRGVCQPSLDYTSYTCKCHSGWEGAQCTEDKDECKKSPCQNGARCVNIVGSYRCECPPGYSGDNCQTNIDDCSSNPCRNGGTCVDKVGRYLCECRAGFYGERCEEEVDECASNPCWNGGHCTDYVNSYTCQCPPGYDGINCERDIPDCTETSCLNNGTCVDGINHFSCRCRQGFSGQYCQFELNECDSHPCKNGGTCIDGLGTFHCNCPLMYNGKTCESMVNLCSQIKCQNGGTCIQKEINWRCSCQPGWTGLYCDIPSMSCHAVALNKGVAENMVCKHAGHCKNIGNTHQCECQKGYTGSYCETEIDECLSSPCRNGGTCVDYQGGYDCKCKAGFQGVNCEYNVNECQSNPCRNGGTCIDLINHFSCSCPPGTKGLQCEVDVDECAPEFGPRCKNGGQCVDGVGRYTCNCPPGFAGEHCEGDVNECRSGPCYSPGTIDCVPLINSYQCRCRLGYTGQRCESMVDLCQSKPCHNNGRCTTNSSSVHGYICTCPPGFSGFNCGNYEMFPCASLRCLNGGQCVEIKSRPQCHCPVGFTGTHCEMLTNHCVCQNGGTCMPDESNKFSCRCPPGFSGETCQETPPSCPYPDCEQRAGDKVCDLRCKNPECDWDGSDCTLHWDKPWKNCKASVSCPDLFHNGRCDPECNNAGCLFDSFECDKHSSVGICKYDKYCADHYANGHCNQGCNTEACGWDGLDCARDTPPKLANGTLVIVVLLQPKELLADLKGFLRSLGTLLHTNLQVKLDEHNKLMVYPFYRNEQNEIQNEIAAMKQRGKRELENEIIGSRVHLEIDNRLCTQNSDECISSADQAAAFIAAEYLKSDLPYPIYSITTKPPKETGFNFLYLVAVAAAIILLILVLGVLAAKRKRKHGTLWLPDGFLPNKESKRREPVGQDDFGMKSMQKPQDGGLLDCSSNHHWSEEDHLPKKPRMEDKPLLPVGVDGVDRREWTLQHHKAADITLTPPQADNDMESLDVNVKGPDGFTPLMLASLRNGSSPDCGSMLGEEEEESGADEPGTNVITDLIGQGASLNAQTDRTGETALHLAARYARADAAKRLLDAGADANAHDNMGRTPLHAAVAADAQGVFQILIRNRATDLDARMNDGTTPLILSARLAVEGMVEELVHCHADVNAVDDHGKSALHWAAAVNNVDATLVLLKNGANRDMQDNKEETPLFLAAREGSFEAAQILLDHYSNRDITDHLDRLPRDTAQERMHHDIVRLLDEYNLVHSPHSGPNHVGNGGGHSSMVCGTNGTGYILGMRPGPQGKKNRRAGGKANGVAAGTAKDLKEMKAKRRKKPTGGEGPSVVPVTTAGGNVSRAAGGLSESSVTMSPVDSLESPHSYAGEPVATSSTANSPPLLSSQSARPMLPPVSHMLGQQQSWVGLSKHSYNGHMFSLMPHHQMSAGHSGMQQHHSPGMSAPMNVTMSREQLPPIVTFQMMPTGSGQSLLKQPQQGQMQAQGQNQPQAQHLHCSQGMMYPIPDVSMQHSLSHALQHPSHAHNHTLQHGLPDGQARQVVSYQPRQSPVDKYPTPPSQHSYATAGSEGTTPGHPAQKPSEHPYLTPSPESPDPWSSSSPHSNSDWSDVTTSPTPLGNPHTMPPSCHTHIPEQAQLQPPSQAAQPTQSQQSQRSSVYA

Target ORF information:

RefSeq Version	NM_001115094.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio notch receptor 2 (notch2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001115094.2

ORF Insert Sequence:

ATGGGCCAAT TACCCGGTGT CTCTTCGGGC GGAGTCATCC TTCTCCTTAT TTGCTTTCTC 
AAAACATCAT ATGCTCTGCA GTGTATCGAC CACGAGAAGC CCTGTGTGAA TGGTACCTGT 
GTCATCTCAA GCAACGGGAC ACACTACTGC AGGTGTGCTC CTGGTTTCTT AGGGGAGTAC 
TGTCATCACA GTGACCCTTG TAATCTGTTG TATTGTAAAA ATGGAGGAGA GTGCAAGGTG 
AGCGTGGCCG GTGTCCCTGG GAGCCCCTCA TGCGTGTGTC CTCTGGGCTT TGCGGGTGAG 
CACTGCGAAA TCCAGAAAAA CTCCACCTGC TACCCCAATA ACCCTTGCAC CAATGGAGGA 
AAGTGCTTGC TCCTTCCCAA CGACCAATAC AAATGCCAGT GTGCCCTTGG GTGGACAGGT 
CAGCATTGTG AGCAAGAGGA CGCATGTCTG TCCAGCCCAT GTGCCAACAA GGGTATATGC 
AATGCTCTGC CAAATCGCGA ATACTCTTGT ACCTGTGCGC CTGGTTTTCA TGGTTCCCGT 
TGTCTTAACG ACACAGACGA GTGTGTGGAC CCGTTAGTAT GTAAGAACAA AGGCGTTTGT 
GTGAACACCA TAGGCTCATA TCGATGCAAC TGCCAACCTG GCTTCACCGG ATTGGACTGC 
GGGAGTCCTT ACTTCCCCTG CTCTCCGTCC CCCTGCCAAA ATGGTGGGAC CTGCCGGCAA 
ACAACAGAAA CCACCTATGT ATGCCACTGC CTGCCAGGTT TTAATGGTAC AAACTGTGAG 
GTGAACATTG ATGACTGTCC AGATCACCGC TGTCAGAATG GAGCCACATG CATGGATGGA 
GTGAACACCT ACAACTGCCA GTGCCCACCT GAATGGACAG GTCAGTTTTG CACTGATGAT 
GTGGATGAAT GCAGACTGCA GCCCAACGCC TGTCAGAACG GAGGCACATG CAGTAACACA 
CGCAACGGCT ACAACTGTGT GTGTGTGAAC GGCTGGAGCG GCCTGGACTG CTCTGAAAAC 
ATTGATGACT GTGCAGCTGA GCCCTGCACC GCTGGCTCCA CTTGCATCGA CCGTGTGGCT 
TCCTTCGTTT GCAGCTGCCC TCCCGGCAAG ACTGGTTTGC TGTGTCACAT TGATGATGCC 
TGCATCAGTA ACCCTTGCAA GATGGGCGCT CATTGCGACA CAAACCCTGT CAATGGCAAA 
TTTAGCTGTA ACTGCCCTTC AGGATACAAG GGCAGCACAT GTGCAGAGGA TATCGACGAG 
TGTGTCATTG GGCCAAACCC ATGTGAGCAT GGAGGCTCAT GTAAGAACAC AGAGGGGTCC 
TTCACTTGTA ACTGCGCTCC AGGTTACACC GGCCCACGTT GTGAGCAGGA CATCAATGAA 
TGTGGCTCCA ACCCCTGCCA GAATGATGCC ACCTGCTTGG ATCAGATAGG AGATTACACC 
TGTATCTGTA TGCCAGGCTT TGATGGAACA CACTGTGAAA ATGACATTAA TGAGTGTCTC 
AGCTCACCTT GTCTTAATAA CGGTAGATGT CTAGACCAGG TCAGCCGATT CGTCTGCGAG 
TGTCCTCAGG GTTTCACTGG GGAAACGTGT CAAGTGGACA TCGATGAGTG TGCCAGCACT 
CCGTGCCATA ATGGTGCCAA GTGTCTTGAC CGTCCTAACG GTTACGAGTG TGAATGTGCT 
GAAGGTTTCA CAGGGCCTTT GTGTATGGAA AACATCAACG ACTGTGAACC CCAACCCTGT 
CATCATGGCA AATGTATAGA TGGAATTGCT ACCTTTCACT GCGATTGTCT CCCAGGTTAT 
ATGGGCCCCA TCTGCAGCGA GCAGATACGT GAGTGCCAGA GTGATCCCTG CCAAAACGGA 
GGTCGCTGTG TTGACATGGT TAACAGATAC CAATGCAACT GCCAGCCGGG TACCGCAGGT 
GAGAACTGTC AGATTAACTA TGATGACTGT GCCAGTAATC CCTGCCAGCA CGGGACCTGT 
GAGGATGGAA TCAATGAGTA CAAATGCGTG TGCGAACCTG GATACACAGG GGAACGATGC 
GCTGATGAGA TCAATGAGTG CAGTTCAAAC CCATGTCAGA GTGGGGGGAC GTGTGAGGAT 
AAAGTGAATG GATTTAAGTG CAAGTGTCCA GTTGGGACTT ATGGGCCCCT GTGTCTCTCA 
GGGGCAGACC GTTGTATCCG CATGCCCTGC CGGCATGGAG AGTGCATTGG GAAGCAGGAA 
GGGTATCTTT GTCAATGTGA GCCAGGCTGG GAGGGTACGA ACTGTGACCA AGAGAAGAAC 
GAGTGTCTGT CCAACCCGTG TCAGAATGGT GGAACCTGTA ATGACCGTCT GAATGATTAT 
ACCTGCGTAT GCGCCCGTGG GTTTGCAGGT TTCAACTGTG AGATCAACAT AAATGAATGT 
GAGTCAAACC CCTGCATGAA TCAGGGAACC TGTGTGGATG GAGTCAACAG CTACGTATGC 
CACTGCAAAC CACCTTACAC AGGGAAACAC TGTCTGGAGA AGTTGATCCC ATGTGCCTCT 
CAGCCCTGTC AGAGACGAGG AGTCTGTCAG CCATCGCTGG ATTACACCAG CTACACCTGC 
AAATGCCACA GCGGCTGGGA GGGAGCCCAA TGCACAGAGG ATAAAGACGA GTGTAAGAAG 
AGCCCTTGTC AGAATGGTGC TCGCTGTGTG AATATAGTTG GCAGCTACCG TTGCGAATGC 
CCGCCTGGAT ACAGCGGGGA CAACTGCCAG ACCAACATCG ATGACTGCAG CTCTAATCCA 
TGCCGTAACG GGGGTACATG TGTTGATAAA GTGGGCCGGT ACTTGTGCGA GTGCAGAGCT 
GGTTTTTATG GCGAGCGCTG TGAGGAAGAG GTGGACGAGT GCGCCAGCAA CCCCTGCTGG 
AATGGAGGAC ACTGCACAGA CTACGTGAAC AGCTACACAT GCCAATGCCC GCCAGGCTAT 
GACGGCATCA ATTGTGAACG TGATATCCCA GACTGCACTG AAACCTCTTG TCTGAACAAT 
GGGACTTGTG TGGATGGAAT TAACCATTTT TCCTGCCGTT GTCGGCAAGG TTTTTCGGGC 
CAGTATTGCC AGTTTGAACT GAACGAGTGT GACTCTCATC CCTGTAAAAA CGGTGGCACC 
TGCATAGACG GTCTGGGGAC TTTCCACTGC AACTGCCCAC TAATGTACAA TGGAAAAACC 
TGTGAGTCAA TGGTGAATTT GTGCAGTCAG ATTAAGTGTC AGAATGGAGG TACTTGCATC 
CAGAAAGAAA TCAATTGGAG ATGCAGCTGT CAGCCTGGCT GGACAGGCCT GTATTGTGAC 
ATCCCCAGCA TGTCCTGCCA CGCTGTTGCC CTCAACAAAG GTGTGGCGGA GAACATGGTC 
TGCAAGCACG CAGGTCATTG TAAGAACATA GGCAACACAC ATCAGTGCGA GTGTCAGAAG 
GGATATACGG GCAGTTACTG CGAGACTGAG ATAGATGAAT GTCTATCCAG CCCTTGCAGA 
AATGGAGGGA CATGTGTGGA CTACCAGGGT GGATATGACT GCAAGTGTAA AGCAGGTTTT 
CAGGGTGTGA ACTGTGAATA TAATGTTAAC GAGTGCCAGT CAAATCCCTG TCGCAATGGA 
GGAACCTGCA TAGATCTGAT CAACCATTTC TCCTGCTCTT GCCCTCCTGG AACCAAAGGT 
TTGCAGTGTG AAGTGGACGT GGATGAATGT GCCCCGGAAT TTGGCCCGCG CTGTAAGAAT 
GGCGGCCAGT GTGTGGATGG TGTGGGCCGG TACACCTGCA ACTGCCCTCC TGGTTTCGCT 
GGAGAGCATT GCGAGGGAGA TGTGAACGAG TGTCGTTCTG GACCATGCTA CTCACCTGGC 
ACCATTGACT GCGTGCCACT TATCAACAGC TATCAATGCC GTTGCCGTCT CGGTTATACT 
GGTCAACGTT GTGAGTCAAT GGTTGATCTG TGCCAGTCCA AGCCATGCCA CAACAATGGT 
AGATGCACTA CGAACTCCAG CTCTGTACAC GGATACATCT GTACATGCCC ACCTGGTTTC 
TCTGGCTTTA ACTGTGGCAA TTATGAGATG TTCCCATGTG CCAGTTTACG CTGTCTGAAT 
GGTGGACAGT GTGTGGAGAT AAAGAGCCGC CCTCAGTGCC ACTGCCCAGT GGGTTTTACT 
GGAACACACT GTGAGATGTT AACAAACCAT TGCGTGTGTC AAAATGGTGG CACCTGCATG 
CCAGATGAGT CCAACAAGTT TAGCTGCCGG TGTCCACCAG GGTTTAGCGG GGAGACCTGT 
CAGGAGACTC CACCTTCTTG CCCTTATCCG GATTGCGAAC AGCGGGCTGG AGACAAAGTA 
TGTGACCTGC GTTGTAAGAA CCCGGAGTGT GACTGGGATG GAAGTGACTG TACGCTCCAC 
TGGGACAAAC CCTGGAAGAA CTGCAAAGCC TCAGTGTCCT GCCCAGACTT ATTTCATAAC 
GGCCGCTGTG ACCCAGAGTG CAACAATGCA GGCTGCCTAT TCGACAGCTT TGAATGCGAC 
AAACATTCAT CGGTGGGAAT TTGCAAATAC GACAAATACT GTGCAGATCA TTACGCCAAT 
GGTCATTGCA ACCAGGGCTG CAACACGGAA GCATGTGGCT GGGATGGTCT GGACTGTGCC 
AGAGACACTC CTCCCAAGCT GGCAAACGGC ACACTGGTGA TTGTGGTTCT GCTGCAACCC 
AAAGAGCTGC TGGCTGACCT TAAGGGTTTC CTGCGTTCTC TGGGGACACT CCTGCACACC 
AACCTGCAAG TGAAGCTAGA TGAGCATAAT AAACTTATGG TGTACCCATT CTATAGAAAT 
GAGCAGAATG AAATTCAAAA TGAAATTGCC GCTATGAAGC AACGTGGAAA ACGCGAGCTG 
GAGAACGAGA TTATCGGATC GAGGGTTCAC CTGGAGATTG ACAATCGGCT GTGTACTCAG 
AACTCAGACG AATGCATCTC CAGTGCAGAC CAGGCTGCAG CCTTTATAGC TGCTGAGTAC 
CTGAAGTCAG ATCTGCCGTA CCCTATCTAC TCCATCACCA CCAAGCCACC TAAAGAAACT 
GGGTTTAATT TCCTGTACCT GGTTGCTGTG GCTGCTGCCA TCATCCTGCT CATTTTGGTG 
CTGGGTGTTT TGGCTGCCAA GCGCAAACGC AAGCACGGCA CTCTGTGGCT TCCTGATGGC 
TTTCTGCCAA ACAAAGAGAG CAAGCGGAGG GAGCCAGTGG GACAGGATGA CTTTGGCATG 
AAGAGCATGC AGAAGCCACA GGATGGAGGA TTATTGGATT GCAGTTCGAA CCATCACTGG 
TCAGAAGAAG ATCATCTTCC TAAAAAACCT AGAATGGAGG ATAAGCCATT GCTTCCTGTT 
GGAGTGGATG GAGTGGACAG GAGAGAGTGG ACCCTGCAGC ATCATAAAGC TGCTGACATC 
ACACTCACCC CCCCTCAGGC AGACAACGAC ATGGAGAGTC TGGACGTCAA TGTTAAAGGG 
CCAGATGGTT TCACTCCTCT CATGCTGGCA TCTCTAAGAA ATGGTAGCAG CCCTGACTGT 
GGCAGCATGC TAGGAGAGGA GGAGGAGGAA AGTGGTGCTG ACGAGCCTGG CACAAACGTC 
ATTACCGACT TAATTGGCCA GGGTGCGTCG CTCAATGCCC AGACTGATCG CACCGGGGAG 
ACTGCACTTC ACCTCGCTGC ACGATACGCT CGAGCAGATG CTGCCAAGAG ACTGCTGGAC 
GCTGGTGCTG ATGCCAATGC TCATGATAAC ATGGGCCGTA CACCTCTTCA TGCGGCCGTA 
GCAGCAGATG CTCAAGGAGT TTTCCAGATT CTCATCCGGA ACCGTGCCAC TGATCTTGAT 
GCCCGTATGA ATGACGGAAC AACACCTCTC ATCCTGTCAG CCAGACTGGC AGTGGAAGGC 
ATGGTGGAGG AACTGGTGCA CTGCCATGCA GACGTTAATG CTGTGGACGA TCATGGCAAG 
TCAGCTCTGC ACTGGGCTGC TGCTGTGAAT AATGTGGATG CCACTTTGGT GCTGCTCAAA 
AATGGAGCCA ACCGAGACAT GCAGGACAAC AAGGAGGAGA CGCCGTTGTT TCTGGCGGCT 
AGAGAAGGCA GCTTTGAGGC TGCTCAAATT CTGCTTGACC ATTACTCAAA CCGTGACATC 
ACCGATCACC TGGACCGCCT GCCGCGTGAC ACCGCACAAG AGCGAATGCA TCATGACATT 
GTGCGTCTGC TGGATGAGTA CAACCTGGTG CACAGCCCAC ACTCCGGGCC CAACCATGTA 
GGAAATGGTG GAGGGCACTC ATCGATGGTC TGTGGCACAA ACGGGACAGG ATACATCCTC 
GGCATGCGTC CTGGACCACA GGGCAAAAAG AACCGCAGAG CTGGAGGAAA AGCAAATGGT 
GTAGCAGCCG GGACAGCCAA AGATCTGAAA GAAATGAAAG CCAAGAGACG GAAGAAACCA 
ACCGGAGGAG AGGGGCCAAG TGTGGTGCCG GTGACCACAG CTGGAGGGAA TGTAAGCAGA 
GCAGCTGGTG GTCTCTCCGA GAGCTCAGTT ACCATGTCTC CTGTGGATTC CCTAGAGTCT 
CCACACTCAT ATGCCGGAGA GCCCGTGGCC ACCTCCAGCA CGGCCAATTC TCCACCTCTG 
CTTAGCAGCC AGTCAGCGAG GCCCATGCTG CCCCCGGTGA GTCACATGCT GGGCCAGCAG 
CAAAGCTGGG TGGGTTTGTC TAAACACAGC TACAATGGCC ACATGTTTAG CCTCATGCCA 
CACCACCAAA TGAGTGCAGG CCATAGTGGC ATGCAGCAGC ACCACAGCCC AGGCATGTCC 
GCCCCAATGA ACGTCACCAT GAGCCGTGAA CAACTCCCGC CAATTGTCAC CTTCCAGATG 
ATGCCGACCG GCAGCGGGCA GAGCCTCCTC AAGCAGCCAC AACAGGGGCA GATGCAGGCC 
CAAGGGCAAA ATCAGCCACA GGCTCAGCAT CTACACTGCA GCCAGGGTAT GATGTACCCC 
ATTCCTGACG TGAGCATGCA GCACAGCCTA TCTCACGCCC TTCAGCACCC TTCTCATGCT 
CACAACCACA CACTCCAGCA TGGGCTGCCT GATGGCCAAG CACGGCAGGT GGTGTCCTAT 
CAGCCACGTC AGAGCCCTGT GGATAAGTAC CCCACGCCAC CTTCCCAGCA CAGCTACGCC 
ACTGCCGGCT CTGAGGGAAC CACCCCTGGA CATCCAGCAC AAAAACCCAG CGAACACCCC 
TACCTCACCC CTTCACCAGA GTCACCCGAC CCCTGGTCCT CCTCGTCCCC ACACTCCAAC 
TCAGACTGGT CCGATGTCAC CACAAGCCCC ACACCTCTAG GAAACCCACA CACAATGCCA 
CCTTCCTGCC ACACACACAT TCCCGAACAG GCCCAGCTGC AGCCCCCATC ACAGGCCGCG 
CAGCCAACAC AGTCCCAGCA GTCCCAGCGC AGCAGTGTGT ACGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis.
Cell reports27(5)1567-1578.e5(2019 04)
Xue Y,Liu D,Cui G,Ding Y,Ai D,Gao S,Zhang Y,Suo S,Wang X,Lv P,Zhou C,Li Y,Chen X,Peng G,Jing N,Han JJ,Liu F

Deficiency of lrp4 in zebrafish and human LRP4 mutation induce aberrant activation of Jagged-Notch signaling in fin and limb development.
Cellular and molecular life sciences : CMLS76(1)163-178(2019 Jan)
Tian J,Shao J,Liu C,Hou HY,Chou CW,Shboul M,Li GQ,El-Khateeb M,Samarah OQ,Kou Y,Chen YH,Chen MJ,Lyu Z,Chen WL,Chen YF,Sun YH,Liu YW

Transcriptomics reveals complex kinetics of dorsal-ventral patterning gene expression in the mandibular arch.
Genesis (New York, N.Y. : 2000)57(1)e23275(2019 01)
Sharma PP,MacLean AL,Meinecke L,Clouthier DE,Nie Q,Schilling TF

Secretogranin-II plays a critical role in zebrafish neurovascular modeling.
Journal of molecular cell biology10(5)388-401(2018 10)
Tao B,Hu H,Mitchell K,Chen J,Jia H,Zhu Z,Trudeau VL,Hu W

Spine Patterning Is Guided by Segmentation of the Notochord Sheath.
Cell reports22(8)2026-2038(2018 02)
Wopat S,Bagwell J,Sumigray KD,Dickson AL,Huitema LFA,Poss KD,Schulte-Merker S,Bagnat M

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notch2 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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