Rbl2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rbl2
Entrez Gene ID	81758
Full Name	RB transcriptional corepressor like 2
Synonyms	Rb2
General protein information	Preferred Names RB transcriptional corepressor like 2 Names retinoblastoma-like protein 2 130 kDa retinoblastoma-associated protein P130 PPAR-alpha-interacting complex protein 128 PRB2 PRIC128 RBR-2 retinoblastoma-like 2 retinoblastoma-related protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19p11
Summary	member of the retinoblastoma gene family; contains an E1A-binding domain [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006255201.3	XP_006255263.1	retinoblastoma-like protein 2 isoform X3
XM_006255200.3	XP_006255262.1	retinoblastoma-like protein 2 isoform X2
XM_006255199.3	XP_006255261.1	retinoblastoma-like protein 2 isoform X1
NM_031094.1	NP_112356.1	retinoblastoma-like protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RBL2	XP_523371.2
Canis lupus familiaris (dog)	RBL2	XP_535303.2
Danio rerio (zebrafish)	rbl2	XP_002667000.3
Macaca mulatta (Rhesus monkey)	RBL2	XP_002802522.1
Drosophila melanogaster (fruit fly)	Rbf	NP_525036.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006477	XP_316517.4
Bos taurus (cattle)	RBL2	NP_001091542.1
Rattus norvegicus (Norway rat)	Rbl2	NP_112356.1
Homo sapiens (human)	RBL2	NP_005602.3
Mus musculus (house mouse)	Rbl2	NP_035380.3
Xenopus tropicalis (tropical clawed frog)	rbl2	XP_002937369.2

Gene Ontology
Bibliography

Related articles in PubMed

Post-transcriptional gene expression control by NANOS is up-regulated and functionally important in pRb-deficient cells.
Miles WO, Korenjak M, Griffiths LM, Dyer MA, Provero P, Dyson NJ
The EMBO journal33(19)2201-15(2014 Oct)

The degradation of cell cycle regulators by SKP2/CKS1 ubiquitin ligase is genetically controlled in rodent liver cancer and contributes to determine the susceptibility to the disease.
Calvisi DF, Pinna F, Ladu S, Muroni MR, Frau M, Demartis I, Tomasi ML, Sini M, Simile MM, Seddaiu MA, Feo F, Pascale RM
International journal of cancer126(5)1275-81(2010 Mar)

In vitro senescence of rat mesenchymal stem cells is accompanied by downregulation of stemness-related and DNA damage repair genes.
Galderisi U, Helmbold H, Squillaro T, Alessio N, Komm N, Khadang B, Cipollaro M, Bohn W, Giordano A
Stem cells and development18(7)1033-42(2009 Sep)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

The PPARalpha/p16INK4a pathway inhibits vascular smooth muscle cell proliferation by repressing cell cycle-dependent telomerase activation.
Gizard F, Nomiyama T, Zhao Y, Findeisen HM, Heywood EB, Jones KL, Staels B, Bruemmer D
Circulation research103(10)1155-63(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rbl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rbl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa38664	XM_006255200.3 Latest version!	Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa38665	XM_006255201.3 Latest version!	Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa07577	NM_031094.1 Latest version!	Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa38663	XM_006255199.3 Latest version!	Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rbl2 ( XM_006255200.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255262.1 Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa38664
Clone ID Related Accession (Same CDS sequence)	XM_006255200.3
Accession Version	XM_006255200.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	retinoblastoma-like protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255200.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTG GAGGCAACCA GTCGTCACCT CCCCCTCCTG CTGCTGCAGC CAGCTCAGAG 
GAAGAGGAGG AGGATGGCGA CACCGCGGAC CGGGCGCAGC CCGCAGGGTC CCCAAGCCAT 
CAGATCCAAC AGCGGTTCGA AGAGCTGTGC AGCCGCCTTA ACATGGACGA GGCGGCACGC 
GCCGAGGCCT GGAGCAGCTA CCGCAGCATG AGCGAGAGCT ACACGCTGGA GGGAAATGAT 
CTCCATTGGT TAGCATGTGC CTTATATGTG GCTTGCAGAA AATCTGTTCC TACTGTGAGC 
AAAGGGACTG CTGAAGGAAA CTATGTGTCT TTAACTAGAA TCCTTCGATG TTCTGAGCAA 
AGCCTAATTG AATTTTTTAA CAAGATGAAG AAGTGGGAAG ATATGGCAAA TTTACCCCCA 
CACTTCCGAG AACGAACCGA AAGATTAGAA AGAAACTTCA CTGTCTCCGC TGTGATCTTT 
AAGAAATATG AACCCATTTT TCAAGACATT TTTAAATATC CCCAAGAAGA ACAACCTCGC 
CAGCAAAGAG GAAGAAAGCA GAGGCGACAG CCCTGTACCA CATCTGAAAT TTTCCATTTT 
TGTTGGGTGC TTTTTATATA CGCAAAAGGA AACTTCCCCA TGATCAGTGA TGATCTGGTC 
AATTCCTACC ATCTTCTGCT GTGTGCACTA GATTTAGTTT ATGGAAATGC CCTACAATGC 
TCTAACCGGA AAGAACTTGT GAACCCTAAT TTTAAAGGCG TGTCCGAGGA CGGCCACCCC 
AGGGACTCTC ACCCGTCCTC TGATCCACCG TGTGTCATTG AGAAGCTCTG CTCCTTACAC 
GACGGCCTAG TGCTGGAGGC AAAGGGGATA AAGCAGCATT TCTGGAAACC CTACATTAGG 
AAGCTTTTTG AAAAGAAGCT CCTCAGGGGG AAGGAAGAAA ATCTCACTGG CTTCCTGGAG 
CCCGGGAACT TCGCAGAGAG CTTTAAGGCT GTTAATAAGG CGTATGAAGA ATACGTGTTA 
GCCACTGGGA GTCTGGATGA GCGGATATTT CTTGGCGAGG ATGCGGAGGA GGAAGTTGGA 
ACTCTCTCAC GGTGTGTAAG TGCTGCCTCG GGCACAGAGA GTGCCGAACG AACGCAGATG 
AGAGACATCT TGCAGCAGCA TCTTGACAAG TCTAAAACAC TTAGAGTCTG CAACCCACTG 
ACTGGCGTGC GGTATGTTCA GGAGAACAGC CCTTGTGTGA CTCCCGTCTC CACAGCAACA 
CACAGCCTGA ACCGTCTTCA CACCATGCTG GCCGGCCTCA GGAATGCACC CAGTGAGAAG 
CTAGAACAGA TACTCAGGTC ATGTTCCCGA GATCCGACTC GGGCTATCGC TGACAGGTTG 
AGAGAGATGT ATGAAATATA TTCTCAGCAT TTCCAGCCAG ACGAGAATGT TAGTAATTGT 
GCTAAAGAAA TGGCCAACAA ACACTTTCGT TTTGCAGAAA TGCTTTACTA TAAAGTATTA 
GAGTCTGTTA TTGAGCAAGA ACAGAAGAGA TTGGGAGACA TGGACTTATC TGGTGTTCTG 
GAGCAAGATG CATTCCATAA GTCACTCCTG GCCTGCTGCC TTGAGGTGGT CGCTTTTTCC 
TATAAGCCTC CTGGAAATTT CCCATTTATT GCTGAAATAT TTGATGTACC ACATTATCAT 
TTTTATAAGG TAATAGAAGT ATTTATTAGA GCAGAAGACG GTCTTTGCAG AGAAGTGGTC 
AAACACCTCA ATCAGATTGA AGAACAAATT TTAGACCACT TGGCATGGAA AACCAAGTCC 
CCACTGTGGG ATAGAATTAG AGATAATGAA AACAGAGTCC CTACATGTGA AGAGGTTACA 
CCACCTCACA ACCTAGAGAG AACAGATGAA ATCTATATTG CTGGCTCTCC CTTAACTCCG 
AGAAGGGTGG GTGAAGTTCG CACTGATGCT GGAGGACTTG GAAGAAGTGT GACATCTCCA 
ACCACATTGT ATGACAGGTA CAGTTCCCCA ACAGTCAGTA CTACTAGAAG GCGGCTATTT 
GAGAGTGACA GTCCCTCTGA AGGAAGCACG GCTGGGCGCA TCCCCCCACA ACCCCTAGTC 
AATGCTGTCC CTGTGCAGAA TGTATCTGGG GAGACTGTTT CTGTCACACC AGTTCCTGGA 
CAGACCTTGG TCACCATGGC AACAGCCACT GTCACAGCCA ACAATGGACA AACAGTGACC 
ATTCCAGTAC AAGGTATTGC CAACGAAAAT GGAGGGATAA CATTCTTCCC AGTCCAAGTG 
AATGTTGGGG GCCAGGCACA GGCTGTGACT GGCTCTATCC AGCCCCTCAG TGCTCAAGCG 
CTGGCTGGAA GTCTGAGTTC CCAACAGGTG ACAGGAACAA CTTTGCAAGT CCCCGGTCCG 
GTGGCCATTC AACAAATTTC CCCTGGAGGA CAACAGCAGA ACCAAGGCCA GCCGTTAACC 
AGCAGCAGTA TCAGGCCTCG GAAGACTAGC TCCTTATCAC TTTTCTTTAG AAAGGTTTAC 
TACTTAGCTG GTGTCCGCCT TCGAGACCTT TGCACAAAAC TAGATATTTC AGATGAACTG 
AGGAAAAAAA TTTGGACCTG CTTTGAATTC TCTATAGTCC AGTGTCCTGA ACTTATGATG 
GACAGACATC TGGACCAGTT GTTAATGTGT GCCATTTACG TGATGGCAAA GGTCACAAAA 
GAAGACAAGT CCTTCCAGAA TATCATGCGC TGTTACAGGA CTCAGCCACA GGCCCGGAGC 
CAGGAGTCAA CAAGAAAGAA TCTAAGGGGG TTGGCTGTCT TCATTTTTTA CATTGCTGGC 
ATACAATTTC ATGCAAGTTA TTTAAAGTCC TCTTCCTTCG AAGGCAATGA TGTGGTATAT 
TTATTTTTAT GTATATGGTG TATTACCTGC ATGTGTATCT GCACCACATG GGTGCCTGGT 
ACCCTCAGAG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255262.1
CDS	124..3078
Translation	MASGGNQSSPPPPAAAASSEEEEEDGDTADRAQPAGSPSHQIQQRFEELCSRLNMDEAARAEAWSSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTAEGNYVSLTRILRCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTTSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGVSEDGHPRDSHPSSDPPCVIEKLCSLHDGLVLEAKGIKQHFWKPYIRKLFEKKLLRGKEENLTGFLEPGNFAESFKAVNKAYEEYVLATGSLDERIFLGEDAEEEVGTLSRCVSAASGTESAERTQMRDILQQHLDKSKTLRVCNPLTGVRYVQENSPCVTPVSTATHSLNRLHTMLAGLRNAPSEKLEQILRSCSRDPTRAIADRLREMYEIYSQHFQPDENVSNCAKEMANKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGVLEQDAFHKSLLACCLEVVAFSYKPPGNFPFIAEIFDVPHYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKTKSPLWDRIRDNENRVPTCEEVTPPHNLERTDEIYIAGSPLTPRRVGEVRTDAGGLGRSVTSPTTLYDRYSSPTVSTTRRRLFESDSPSEGSTAGRIPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGPVAIQQISPGGQQQNQGQPLTSSSIRPRKTSSLSLFFRKVYYLAGVRLRDLCTKLDISDELRKKIWTCFEFSIVQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQESTRKNLRGLAVFIFYIAGIQFHASYLKSSSFEGNDVVYLFLCIWCITCMCICTTWVPGTLRG

Target ORF information:

RefSeq Version	XM_006255200.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255200.3

ORF Insert Sequence:

ATGGCATCTG GAGGCAACCA GTCGTCACCT CCCCCTCCTG CTGCTGCAGC CAGCTCAGAG 
GAAGAGGAGG AGGATGGCGA CACCGCGGAC CGGGCGCAGC CCGCAGGGTC CCCAAGCCAT 
CAGATCCAAC AGCGGTTCGA AGAGCTGTGC AGCCGCCTTA ACATGGACGA GGCGGCACGC 
GCCGAGGCCT GGAGCAGCTA CCGCAGCATG AGCGAGAGCT ACACGCTGGA GGGAAATGAT 
CTCCATTGGT TAGCATGTGC CTTATATGTG GCTTGCAGAA AATCTGTTCC TACTGTGAGC 
AAAGGGACTG CTGAAGGAAA CTATGTGTCT TTAACTAGAA TCCTTCGATG TTCTGAGCAA 
AGCCTAATTG AATTTTTTAA CAAGATGAAG AAGTGGGAAG ATATGGCAAA TTTACCCCCA 
CACTTCCGAG AACGAACCGA AAGATTAGAA AGAAACTTCA CTGTCTCCGC TGTGATCTTT 
AAGAAATATG AACCCATTTT TCAAGACATT TTTAAATATC CCCAAGAAGA ACAACCTCGC 
CAGCAAAGAG GAAGAAAGCA GAGGCGACAG CCCTGTACCA CATCTGAAAT TTTCCATTTT 
TGTTGGGTGC TTTTTATATA CGCAAAAGGA AACTTCCCCA TGATCAGTGA TGATCTGGTC 
AATTCCTACC ATCTTCTGCT GTGTGCACTA GATTTAGTTT ATGGAAATGC CCTACAATGC 
TCTAACCGGA AAGAACTTGT GAACCCTAAT TTTAAAGGCG TGTCCGAGGA CGGCCACCCC 
AGGGACTCTC ACCCGTCCTC TGATCCACCG TGTGTCATTG AGAAGCTCTG CTCCTTACAC 
GACGGCCTAG TGCTGGAGGC AAAGGGGATA AAGCAGCATT TCTGGAAACC CTACATTAGG 
AAGCTTTTTG AAAAGAAGCT CCTCAGGGGG AAGGAAGAAA ATCTCACTGG CTTCCTGGAG 
CCCGGGAACT TCGCAGAGAG CTTTAAGGCT GTTAATAAGG CGTATGAAGA ATACGTGTTA 
GCCACTGGGA GTCTGGATGA GCGGATATTT CTTGGCGAGG ATGCGGAGGA GGAAGTTGGA 
ACTCTCTCAC GGTGTGTAAG TGCTGCCTCG GGCACAGAGA GTGCCGAACG AACGCAGATG 
AGAGACATCT TGCAGCAGCA TCTTGACAAG TCTAAAACAC TTAGAGTCTG CAACCCACTG 
ACTGGCGTGC GGTATGTTCA GGAGAACAGC CCTTGTGTGA CTCCCGTCTC CACAGCAACA 
CACAGCCTGA ACCGTCTTCA CACCATGCTG GCCGGCCTCA GGAATGCACC CAGTGAGAAG 
CTAGAACAGA TACTCAGGTC ATGTTCCCGA GATCCGACTC GGGCTATCGC TGACAGGTTG 
AGAGAGATGT ATGAAATATA TTCTCAGCAT TTCCAGCCAG ACGAGAATGT TAGTAATTGT 
GCTAAAGAAA TGGCCAACAA ACACTTTCGT TTTGCAGAAA TGCTTTACTA TAAAGTATTA 
GAGTCTGTTA TTGAGCAAGA ACAGAAGAGA TTGGGAGACA TGGACTTATC TGGTGTTCTG 
GAGCAAGATG CATTCCATAA GTCACTCCTG GCCTGCTGCC TTGAGGTGGT CGCTTTTTCC 
TATAAGCCTC CTGGAAATTT CCCATTTATT GCTGAAATAT TTGATGTACC ACATTATCAT 
TTTTATAAGG TAATAGAAGT ATTTATTAGA GCAGAAGACG GTCTTTGCAG AGAAGTGGTC 
AAACACCTCA ATCAGATTGA AGAACAAATT TTAGACCACT TGGCATGGAA AACCAAGTCC 
CCACTGTGGG ATAGAATTAG AGATAATGAA AACAGAGTCC CTACATGTGA AGAGGTTACA 
CCACCTCACA ACCTAGAGAG AACAGATGAA ATCTATATTG CTGGCTCTCC CTTAACTCCG 
AGAAGGGTGG GTGAAGTTCG CACTGATGCT GGAGGACTTG GAAGAAGTGT GACATCTCCA 
ACCACATTGT ATGACAGGTA CAGTTCCCCA ACAGTCAGTA CTACTAGAAG GCGGCTATTT 
GAGAGTGACA GTCCCTCTGA AGGAAGCACG GCTGGGCGCA TCCCCCCACA ACCCCTAGTC 
AATGCTGTCC CTGTGCAGAA TGTATCTGGG GAGACTGTTT CTGTCACACC AGTTCCTGGA 
CAGACCTTGG TCACCATGGC AACAGCCACT GTCACAGCCA ACAATGGACA AACAGTGACC 
ATTCCAGTAC AAGGTATTGC CAACGAAAAT GGAGGGATAA CATTCTTCCC AGTCCAAGTG 
AATGTTGGGG GCCAGGCACA GGCTGTGACT GGCTCTATCC AGCCCCTCAG TGCTCAAGCG 
CTGGCTGGAA GTCTGAGTTC CCAACAGGTG ACAGGAACAA CTTTGCAAGT CCCCGGTCCG 
GTGGCCATTC AACAAATTTC CCCTGGAGGA CAACAGCAGA ACCAAGGCCA GCCGTTAACC 
AGCAGCAGTA TCAGGCCTCG GAAGACTAGC TCCTTATCAC TTTTCTTTAG AAAGGTTTAC 
TACTTAGCTG GTGTCCGCCT TCGAGACCTT TGCACAAAAC TAGATATTTC AGATGAACTG 
AGGAAAAAAA TTTGGACCTG CTTTGAATTC TCTATAGTCC AGTGTCCTGA ACTTATGATG 
GACAGACATC TGGACCAGTT GTTAATGTGT GCCATTTACG TGATGGCAAA GGTCACAAAA 
GAAGACAAGT CCTTCCAGAA TATCATGCGC TGTTACAGGA CTCAGCCACA GGCCCGGAGC 
CAGGAGTCAA CAAGAAAGAA TCTAAGGGGG TTGGCTGTCT TCATTTTTTA CATTGCTGGC 
ATACAATTTC ATGCAAGTTA TTTAAAGTCC TCTTCCTTCG AAGGCAATGA TGTGGTATAT 
TTATTTTTAT GTATATGGTG TATTACCTGC ATGTGTATCT GCACCACATG GGTGCCTGGT 
ACCCTCAGAG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rbl2 ( XM_006255201.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255263.1 Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa38665
Clone ID Related Accession (Same CDS sequence)	XM_006255201.3
Accession Version	XM_006255201.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	retinoblastoma-like protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255201.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGTG ATGATCTGGT CAATTCCTAC CATCTTCTGC TGTGTGCACT AGATTTAGTT 
TATGGAAATG CCCTACAATG CTCTAACCGG AAAGAACTTG TGAACCCTAA TTTTAAAGGC 
GTGTCCGAGG ACGGCCACCC CAGGGACTCT CACCCGTCCT CTGATCCACC GTGTGTCATT 
GAGAAGCTCT GCTCCTTACA CGACGGCCTA GTGCTGGAGG CAAAGGGGAT AAAGCAGCAT 
TTCTGGAAAC CCTACATTAG GAAGCTTTTT GAAAAGAAGC TCCTCAGGGG GAAGGAAGAA 
AATCTCACTG GCTTCCTGGA GCCCGGGAAC TTCGCAGAGA GCTTTAAGGC TGTTAATAAG 
GCGTATGAAG AATACGTGTT AGCCACTGGG AGTCTGGATG AGCGGATATT TCTTGGCGAG 
GATGCGGAGG AGGAAGTTGG AACTCTCTCA CGGTGTGTAA GTGCTGCCTC GGGCACAGAG 
AGTGCCGAAC GAACGCAGAT GAGAGACATC TTGCAGCAGC ATCTTGACAA GTCTAAAACA 
CTTAGAGTCT GCAACCCACT GACTGGCGTG CGGTATGTTC AGGAGAACAG CCCTTGTGTG 
ACTCCCGTCT CCACAGCAAC ACACAGCCTG AACCGTCTTC ACACCATGCT GGCCGGCCTC 
AGGAATGCAC CCAGTGAGAA GCTAGAACAG ATACTCAGGT CATGTTCCCG AGATCCGACT 
CGGGCTATCG CTGACAGGTT GAGAGAGATG TATGAAATAT ATTCTCAGCA TTTCCAGCCA 
GACGAGAATG TTAGTAATTG TGCTAAAGAA ATGGCCAACA AACACTTTCG TTTTGCAGAA 
ATGCTTTACT ATAAAGTATT AGAGTCTGTT ATTGAGCAAG AACAGAAGAG ATTGGGAGAC 
ATGGACTTAT CTGGTGTTCT GGAGCAAGAT GCATTCCATA AGTCACTCCT GGCCTGCTGC 
CTTGAGGTGG TCGCTTTTTC CTATAAGCCT CCTGGAAATT TCCCATTTAT TGCTGAAATA 
TTTGATGTAC CACATTATCA TTTTTATAAG GTAATAGAAG TATTTATTAG AGCAGAAGAC 
GGTCTTTGCA GAGAAGTGGT CAAACACCTC AATCAGATTG AAGAACAAAT TTTAGACCAC 
TTGGCATGGA AAACCAAGTC CCCACTGTGG GATAGAATTA GAGATAATGA AAACAGAGTC 
CCTACATGTG AAGAGGTTAC ACCACCTCAC AACCTAGAGA GAACAGATGA AATCTATATT 
GCTGGCTCTC CCTTAACTCC GAGAAGGGTG GGTGAAGTTC GCACTGATGC TGGAGGACTT 
GGAAGAAGTG TGACATCTCC AACCACATTG TATGACAGGT ACAGTTCCCC AACAGTCAGT 
ACTACTAGAA GGCGGCTATT TGAGAGTGAC AGTCCCTCTG AAGGAAGCAC GGCTGGGCGC 
ATCCCCCCAC AACCCCTAGT CAATGCTGTC CCTGTGCAGA ATGTATCTGG GGAGACTGTT 
TCTGTCACAC CAGTTCCTGG ACAGACCTTG GTCACCATGG CAACAGCCAC TGTCACAGCC 
AACAATGGAC AAACAGTGAC CATTCCAGTA CAAGGTATTG CCAACGAAAA TGGAGGGATA 
ACATTCTTCC CAGTCCAAGT GAATGTTGGG GGCCAGGCAC AGGCTGTGAC TGGCTCTATC 
CAGCCCCTCA GTGCTCAAGC GCTGGCTGGA AGTCTGAGTT CCCAACAGGT GACAGGAACA 
ACTTTGCAAG TCCCCGGTCC GGTGGCCATT CAACAAATTT CCCCTGGAGG ACAACAGCAG 
AACCAAGGCC AGCCGTTAAC CAGCAGCAGT ATCAGGCCTC GGAAGACTAG CTCCTTATCA 
CTTTTCTTTA GAAAGGTTTA CTACTTAGCT GGTGTCCGCC TTCGAGACCT TTGCACAAAA 
CTAGATATTT CAGATGAACT GAGGAAAAAA ATTTGGACCT GCTTTGAATT CTCTATAGTC 
CAGTGTCCTG AACTTATGAT GGACAGACAT CTGGACCAGT TGTTAATGTG TGCCATTTAC 
GTGATGGCAA AGGTCACAAA AGAAGACAAG TCCTTCCAGA ATATCATGCG CTGTTACAGG 
ACTCAGCCAC AGGCCCGGAG CCAGGTATAC AGAAGTGTCT TGATAAAAGG GAAAAGAAGA 
AATTCTGGCA GCTGTGAGAA CAGAAGCCAT CAGAATTCTC CAACAGAACT AAATACAGAC 
AGAGCCAGTA GAGACTCCAG TCCAGTTATG AGGTCAAACA GCACCTTACC AGTTCCACAG 
CCCAGCAGTG CCCCTCCTAC ACCAACTCGA CTCACAGGTG CCAACAGTGA CATTGAAGAG 
GAGGAAAGAG GAGACCTCAT TCAGTTCTAC AACAACATCT ACAGGAAACA AATCCAAACG 
TTTGCCATGA AGTACTCACA GGCAAACTCC CAACAGATGG ACACCCCTCC CCTGTCTCCC 
TATCCGTTTG TAAGAACGGG CTCCCCTCGC CGAGTACAGT TATCTCAAAG TCACCCTATC 
TACATTTCCC CACATAAAAA CGAAGCAATG CTTTCTCCTC GAGAGAAGAT TTTTTACTAC 
TTCAGCAACA GCCCATCAAA GAGACTGAGA GAAATTAACA GTATGATACG GACAGGAGAG 
ACTCCAACTA AAAAGAGAGG GATTCTCTTG GACGATGGAA GTGAATCGCC TGCAAAAAGA 
ATTTGCCCAG AAAATCATTC TGCTCTGTTA CGTCGTCTCC AAGATGTAGC TAATGACCGA 
GGTTCACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255263.1
CDS	839..3610
Translation	MISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGVSEDGHPRDSHPSSDPPCVIEKLCSLHDGLVLEAKGIKQHFWKPYIRKLFEKKLLRGKEENLTGFLEPGNFAESFKAVNKAYEEYVLATGSLDERIFLGEDAEEEVGTLSRCVSAASGTESAERTQMRDILQQHLDKSKTLRVCNPLTGVRYVQENSPCVTPVSTATHSLNRLHTMLAGLRNAPSEKLEQILRSCSRDPTRAIADRLREMYEIYSQHFQPDENVSNCAKEMANKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGVLEQDAFHKSLLACCLEVVAFSYKPPGNFPFIAEIFDVPHYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKTKSPLWDRIRDNENRVPTCEEVTPPHNLERTDEIYIAGSPLTPRRVGEVRTDAGGLGRSVTSPTTLYDRYSSPTVSTTRRRLFESDSPSEGSTAGRIPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGPVAIQQISPGGQQQNQGQPLTSSSIRPRKTSSLSLFFRKVYYLAGVRLRDLCTKLDISDELRKKIWTCFEFSIVQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRRNSGSCENRSHQNSPTELNTDRASRDSSPVMRSNSTLPVPQPSSAPPTPTRLTGANSDIEEEERGDLIQFYNNIYRKQIQTFAMKYSQANSQQMDTPPLSPYPFVRTGSPRRVQLSQSHPIYISPHKNEAMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLDDGSESPAKRICPENHSALLRRLQDVANDRGSH

Target ORF information:

RefSeq Version	XM_006255201.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255201.3

ORF Insert Sequence:

ATGATCAGTG ATGATCTGGT CAATTCCTAC CATCTTCTGC TGTGTGCACT AGATTTAGTT 
TATGGAAATG CCCTACAATG CTCTAACCGG AAAGAACTTG TGAACCCTAA TTTTAAAGGC 
GTGTCCGAGG ACGGCCACCC CAGGGACTCT CACCCGTCCT CTGATCCACC GTGTGTCATT 
GAGAAGCTCT GCTCCTTACA CGACGGCCTA GTGCTGGAGG CAAAGGGGAT AAAGCAGCAT 
TTCTGGAAAC CCTACATTAG GAAGCTTTTT GAAAAGAAGC TCCTCAGGGG GAAGGAAGAA 
AATCTCACTG GCTTCCTGGA GCCCGGGAAC TTCGCAGAGA GCTTTAAGGC TGTTAATAAG 
GCGTATGAAG AATACGTGTT AGCCACTGGG AGTCTGGATG AGCGGATATT TCTTGGCGAG 
GATGCGGAGG AGGAAGTTGG AACTCTCTCA CGGTGTGTAA GTGCTGCCTC GGGCACAGAG 
AGTGCCGAAC GAACGCAGAT GAGAGACATC TTGCAGCAGC ATCTTGACAA GTCTAAAACA 
CTTAGAGTCT GCAACCCACT GACTGGCGTG CGGTATGTTC AGGAGAACAG CCCTTGTGTG 
ACTCCCGTCT CCACAGCAAC ACACAGCCTG AACCGTCTTC ACACCATGCT GGCCGGCCTC 
AGGAATGCAC CCAGTGAGAA GCTAGAACAG ATACTCAGGT CATGTTCCCG AGATCCGACT 
CGGGCTATCG CTGACAGGTT GAGAGAGATG TATGAAATAT ATTCTCAGCA TTTCCAGCCA 
GACGAGAATG TTAGTAATTG TGCTAAAGAA ATGGCCAACA AACACTTTCG TTTTGCAGAA 
ATGCTTTACT ATAAAGTATT AGAGTCTGTT ATTGAGCAAG AACAGAAGAG ATTGGGAGAC 
ATGGACTTAT CTGGTGTTCT GGAGCAAGAT GCATTCCATA AGTCACTCCT GGCCTGCTGC 
CTTGAGGTGG TCGCTTTTTC CTATAAGCCT CCTGGAAATT TCCCATTTAT TGCTGAAATA 
TTTGATGTAC CACATTATCA TTTTTATAAG GTAATAGAAG TATTTATTAG AGCAGAAGAC 
GGTCTTTGCA GAGAAGTGGT CAAACACCTC AATCAGATTG AAGAACAAAT TTTAGACCAC 
TTGGCATGGA AAACCAAGTC CCCACTGTGG GATAGAATTA GAGATAATGA AAACAGAGTC 
CCTACATGTG AAGAGGTTAC ACCACCTCAC AACCTAGAGA GAACAGATGA AATCTATATT 
GCTGGCTCTC CCTTAACTCC GAGAAGGGTG GGTGAAGTTC GCACTGATGC TGGAGGACTT 
GGAAGAAGTG TGACATCTCC AACCACATTG TATGACAGGT ACAGTTCCCC AACAGTCAGT 
ACTACTAGAA GGCGGCTATT TGAGAGTGAC AGTCCCTCTG AAGGAAGCAC GGCTGGGCGC 
ATCCCCCCAC AACCCCTAGT CAATGCTGTC CCTGTGCAGA ATGTATCTGG GGAGACTGTT 
TCTGTCACAC CAGTTCCTGG ACAGACCTTG GTCACCATGG CAACAGCCAC TGTCACAGCC 
AACAATGGAC AAACAGTGAC CATTCCAGTA CAAGGTATTG CCAACGAAAA TGGAGGGATA 
ACATTCTTCC CAGTCCAAGT GAATGTTGGG GGCCAGGCAC AGGCTGTGAC TGGCTCTATC 
CAGCCCCTCA GTGCTCAAGC GCTGGCTGGA AGTCTGAGTT CCCAACAGGT GACAGGAACA 
ACTTTGCAAG TCCCCGGTCC GGTGGCCATT CAACAAATTT CCCCTGGAGG ACAACAGCAG 
AACCAAGGCC AGCCGTTAAC CAGCAGCAGT ATCAGGCCTC GGAAGACTAG CTCCTTATCA 
CTTTTCTTTA GAAAGGTTTA CTACTTAGCT GGTGTCCGCC TTCGAGACCT TTGCACAAAA 
CTAGATATTT CAGATGAACT GAGGAAAAAA ATTTGGACCT GCTTTGAATT CTCTATAGTC 
CAGTGTCCTG AACTTATGAT GGACAGACAT CTGGACCAGT TGTTAATGTG TGCCATTTAC 
GTGATGGCAA AGGTCACAAA AGAAGACAAG TCCTTCCAGA ATATCATGCG CTGTTACAGG 
ACTCAGCCAC AGGCCCGGAG CCAGGTATAC AGAAGTGTCT TGATAAAAGG GAAAAGAAGA 
AATTCTGGCA GCTGTGAGAA CAGAAGCCAT CAGAATTCTC CAACAGAACT AAATACAGAC 
AGAGCCAGTA GAGACTCCAG TCCAGTTATG AGGTCAAACA GCACCTTACC AGTTCCACAG 
CCCAGCAGTG CCCCTCCTAC ACCAACTCGA CTCACAGGTG CCAACAGTGA CATTGAAGAG 
GAGGAAAGAG GAGACCTCAT TCAGTTCTAC AACAACATCT ACAGGAAACA AATCCAAACG 
TTTGCCATGA AGTACTCACA GGCAAACTCC CAACAGATGG ACACCCCTCC CCTGTCTCCC 
TATCCGTTTG TAAGAACGGG CTCCCCTCGC CGAGTACAGT TATCTCAAAG TCACCCTATC 
TACATTTCCC CACATAAAAA CGAAGCAATG CTTTCTCCTC GAGAGAAGAT TTTTTACTAC 
TTCAGCAACA GCCCATCAAA GAGACTGAGA GAAATTAACA GTATGATACG GACAGGAGAG 
ACTCCAACTA AAAAGAGAGG GATTCTCTTG GACGATGGAA GTGAATCGCC TGCAAAAAGA 
ATTTGCCCAG AAAATCATTC TGCTCTGTTA CGTCGTCTCC AAGATGTAGC TAATGACCGA 
GGTTCACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rbl2 ( NM_031094.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112356.1 Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), mRNA.

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CloneID	ORa07577
Clone ID Related Accession (Same CDS sequence)	NM_031094.1
Accession Version	NM_031094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564761600000
Organism	Rattus norvegicus(Norway rat)
Product	retinoblastoma-like protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D55627.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D55627.1, BC062040.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCATCTG GAGGCAACCA GTCGTCACCT CCTCCTCCTG CTGCTGCAGC CAGCTCAGAG 
GAAGAGGAGG AGGATGGCGA CACCGCGGAC CGGGCGCAGC CCGCAGGGTC CCCAAGCCAT 
CAGATCCAAC AGCGGTTCGA AGAGCTGTGC AGCCGCCTTA ACATGGACGA GGCGGCACGG 
GCCGAGGCCT GGAGCAGCTA CCGCAGCATG AGCGAGAGCT ACACGCTGGA GGGAAATGAT 
CTCCATTGGT TAGCATGTGC CTTATATGTG GCTTGCAGAA AATCTGTTCC TACTGTGAGC 
AAAGGGACTG CTGAAGGAAA CTATGTGTCT TTAACTAGAA TCCTTCGATG TTCTGAGCAA 
AGCCTAATTG AATTTTTTAA CAAGATGAAG AAGTGGGAAG ATATGGCAAA TTTACCCCCA 
CACTTCCGAG AACGAACCGA AAGATTAGAA AGAAACTTCA CTGTCTCCGC TGTGATCTTT 
AAGAAATATG AACCCATTTT TCAAGACATT TTTAAATATC CCCAAGAAGA ACAACCTCGC 
CAGCAAAGAG GAAGAAAGCA GAGGCGACAG CCCTGTACCA CATCTGAAAT TTTCCATTTT 
TGTTGGGTGC TTTTTATATA CGCAAAAGGA AACTTCCCCA TGATCAGTGA TGATCTGGTC 
AATTCCTACC ATCTTCTGCT GTGTGCACTA GATTTAGTTT ATGGAAATGC CCTACAATGC 
TCTAACCGGA AAGAACTTGT GAACCCTAAT TTTAAAGGCG TGTCCGAGGA CGGCCACCCC 
AGGGACTCTC ACCCGTCCTC TGATCCACCG TGTGTCATTG AGAAGCTCTG CTCCTTACAC 
GACGGCCTAG TGCTGGAGGC AAAGGGGATA AAGCAGCATT TCTGGAAACC CTACATTAGG 
AAGCTTTTTG AAAAGAAGCT CCTCAGGGGG AAGGAAGAAA ATCTCACTGG CTTCCTGGAG 
CCCGGGAACT TCGCAGAGAG CTTTAAGGCT GTTAATAAGG CGTATGAAGA ATACGTGTTA 
GCCACTGGGA GTCTGGATGA GCGGATATTT CTTGGCGAGG ATGCGGAGGA GGAAGTTGGA 
ACTTTCTCAC GGTGTGTAAG TGCTGCCTCG GGCACAGAGA GTGCCGAACG AACGCAGATG 
AGAGACATCT TGCAGCAGCA TCTTGACAAG TCTAAAACAC TTAGAGTCTG CAACCCACTG 
ACTGGCGTGC GGTATGTTCA GGAGAACAGC CCTTGTGTGA CTCCCGTCTC CACAGCAACA 
CACAGCCTGA ACCGTCTTCA CACCATGCTG GCCGGCCTCA GGAATGCACC CAGTGAGAAG 
CTAGAACAGA TACTCAGGTC ATGTTCCCGA GATCCGACTC GGGCTATCGC TGACAGGTTG 
AGAGAGATGT ATGAAATATA TTCTCAGCAT TTCCAGCCAG ACGAGAATGT TAGTAATTGT 
GCTAAAGAAA TGGCCAACAA ACACTTTCGT TTTGCAGAAA TGCTTTACTA TAAAGTATTA 
GAGTCTGTTA TTGAGCAAGA ACAGAAGAGA TTGGGAGACA TGGACTTATC TGGTGTTCTG 
GAGCAAGATG CATTCCATAA GTCACTCCTG GCCTGCTGCC TTGAGGTGGT CGCTTTTTCC 
TATAAGCCTC CTGGAAATTT CCCATTTATT GCTGAAATAT TTGATGTACC ACATTATCAT 
TTTTATAAGG TAATAGAAGT ATTTATTAGA GCAGAAGACG GTCTTTGCAG AGAAGTGGTC 
AAACACCTCA ATCAGATTGA AGAACAAATT TTAGACCACT TGGCATGGAA AACCAAGTCC 
CCACTGTGGG ATAGAATTAG AGATAATGAA AACAGAGTCC CTACATGTGA AGAGGTTACA 
CCACCTCACA ACCTAGAGAG AACAGATGAA ATCTATATTG CTGGCTCTCC CTTAACTCCG 
AGAAGGGTGG GTGAAGTTCG CACTGATGCT GGAGGACTTG GAAGAAGTGT GACATCTCCA 
ACCACACTGT ATGACAGGTA CAGTTCCCCA ACAGTCAGTA CTACTAGAAG GCGGCTATTT 
GAGAGTGACA GTCCCTCTGA AGGAAGCACG GCTGGGCGCA TCCCCCCACA ACCCCTAGTC 
AATGCTGTCC CTGTGCAGAA TGTATCTGGG GAGACTGTTT CTGTCACACC AGTTCCTGGA 
CAGACCTTGG TCACCATGGC AACAGCCACT GTCACAGCCA ACAATGGACA AACAGTGACC 
ATTCCAGTAC AAGGTATTGC CAACGAAAAT GGAGGGATAA CATTCTTCCC AGTCCAAGTG 
AATGTTGGGG GCCAGGCACA GGCTGTGACT GGCTCTATCC AGCCCCTCAG TGCTCAAGCG 
CTGGCTGGAA GTCTGAGTTC CCAACAGGTG ACAGGAACAA CTTTGCAAGT CCCCGGTCCG 
GTGGCCATTC AACAAATTTC CCCTGGAGGA CAACAGCAGA ACCAAGGCCA GCCGTTAACC 
AGCAGCAGTA TCAGGCCTCG GAAGACTAGC TCCTTATCAC TTTTCTTTAG AAAGGTTTAC 
TACTTAGCTG GTGTCCGCCT TCGAGACCTT TGTACAAAAC TAGATATTTC AGATGAACTG 
AGGAAAAAAA TTTGGACCTG CTTTGAATTC TCTATAGTCC AGTGTCCTGA ACTTATGATG 
GACAGACATC TGGACCAGTT GTTAATGTGT GCCATTTACG TGATGGCAAA GGTCACAAAA 
GAAGACAAGT CCTTCCAGAA TATCATGCGC TGTTACAGGA CTCAGCCACA GGCCCGGAGC 
CAGGTATACA GAAGTGTCTT GATAAAAGGG AAAAGAAGAA ATTCTGGCAG CTGTGAGAAC 
AGAAGCCATC AGAATTCTCC AACAGAACTA AATACAGACA GAGCCAGTAG AGACTCCAGT 
CCAGTTATGA GGTCAAACAG CACCTTACCA GTTCCACAGC CCAGCAGTGC CCCTCCTACA 
CCAACTCGAC TCACAGGTGC CAACAGTGAC ATTGAAGAGG AGGAAAGAGG AGACCTCATT 
CAGTTCTACA ACAACATCTA CAGGAAACAA ATCCAAACGT TTGCCATGAA GTACTCACAG 
GCAAACTCCC AAATGGACAC CCCTCCCCTG TCTCCCTATC CGTTTGTAAG AACGGGCTCC 
CCTCGCCGAG TACAGTTATC TCAAAGTCAC CCTATCTACA TTTCCCCACA TAAAAACGAA 
GCAATGCTTT CTCCTCGAGA GAAGATTTTT TACTACTTCA GCAACAGCCC ATCAAAGAGA 
CTGAGAGAAA TTAACAGTAT GATACGGACA GGAGAGACTC CAACTAAAAA GAGAGGGATT 
CTCTTGGACG ATGGAAGTGA ATCGCCTGCA AAAAGAATTT GCCCAGAAAA TCATTCTGCT 
CTGTTACGTC GTCTCCAAGA TGTAGCTAAT GACCGAGGTT CACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112356.1
CDS	67..3474
Translation	MASGGNQSSPPPPAAAASSEEEEEDGDTADRAQPAGSPSHQIQQRFEELCSRLNMDEAARAEAWSSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTAEGNYVSLTRILRCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTTSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGVSEDGHPRDSHPSSDPPCVIEKLCSLHDGLVLEAKGIKQHFWKPYIRKLFEKKLLRGKEENLTGFLEPGNFAESFKAVNKAYEEYVLATGSLDERIFLGEDAEEEVGTFSRCVSAASGTESAERTQMRDILQQHLDKSKTLRVCNPLTGVRYVQENSPCVTPVSTATHSLNRLHTMLAGLRNAPSEKLEQILRSCSRDPTRAIADRLREMYEIYSQHFQPDENVSNCAKEMANKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGVLEQDAFHKSLLACCLEVVAFSYKPPGNFPFIAEIFDVPHYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKTKSPLWDRIRDNENRVPTCEEVTPPHNLERTDEIYIAGSPLTPRRVGEVRTDAGGLGRSVTSPTTLYDRYSSPTVSTTRRRLFESDSPSEGSTAGRIPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGPVAIQQISPGGQQQNQGQPLTSSSIRPRKTSSLSLFFRKVYYLAGVRLRDLCTKLDISDELRKKIWTCFEFSIVQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRRNSGSCENRSHQNSPTELNTDRASRDSSPVMRSNSTLPVPQPSSAPPTPTRLTGANSDIEEEERGDLIQFYNNIYRKQIQTFAMKYSQANSQMDTPPLSPYPFVRTGSPRRVQLSQSHPIYISPHKNEAMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLDDGSESPAKRICPENHSALLRRLQDVANDRGSH

Target ORF information:

RefSeq Version	NM_031094.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031094.1

ORF Insert Sequence:

ATGGCATCTG GAGGCAACCA GTCGTCACCT CCTCCTCCTG CTGCTGCAGC CAGCTCAGAG 
GAAGAGGAGG AGGATGGCGA CACCGCGGAC CGGGCGCAGC CCGCAGGGTC CCCAAGCCAT 
CAGATCCAAC AGCGGTTCGA AGAGCTGTGC AGCCGCCTTA ACATGGACGA GGCGGCACGG 
GCCGAGGCCT GGAGCAGCTA CCGCAGCATG AGCGAGAGCT ACACGCTGGA GGGAAATGAT 
CTCCATTGGT TAGCATGTGC CTTATATGTG GCTTGCAGAA AATCTGTTCC TACTGTGAGC 
AAAGGGACTG CTGAAGGAAA CTATGTGTCT TTAACTAGAA TCCTTCGATG TTCTGAGCAA 
AGCCTAATTG AATTTTTTAA CAAGATGAAG AAGTGGGAAG ATATGGCAAA TTTACCCCCA 
CACTTCCGAG AACGAACCGA AAGATTAGAA AGAAACTTCA CTGTCTCCGC TGTGATCTTT 
AAGAAATATG AACCCATTTT TCAAGACATT TTTAAATATC CCCAAGAAGA ACAACCTCGC 
CAGCAAAGAG GAAGAAAGCA GAGGCGACAG CCCTGTACCA CATCTGAAAT TTTCCATTTT 
TGTTGGGTGC TTTTTATATA CGCAAAAGGA AACTTCCCCA TGATCAGTGA TGATCTGGTC 
AATTCCTACC ATCTTCTGCT GTGTGCACTA GATTTAGTTT ATGGAAATGC CCTACAATGC 
TCTAACCGGA AAGAACTTGT GAACCCTAAT TTTAAAGGCG TGTCCGAGGA CGGCCACCCC 
AGGGACTCTC ACCCGTCCTC TGATCCACCG TGTGTCATTG AGAAGCTCTG CTCCTTACAC 
GACGGCCTAG TGCTGGAGGC AAAGGGGATA AAGCAGCATT TCTGGAAACC CTACATTAGG 
AAGCTTTTTG AAAAGAAGCT CCTCAGGGGG AAGGAAGAAA ATCTCACTGG CTTCCTGGAG 
CCCGGGAACT TCGCAGAGAG CTTTAAGGCT GTTAATAAGG CGTATGAAGA ATACGTGTTA 
GCCACTGGGA GTCTGGATGA GCGGATATTT CTTGGCGAGG ATGCGGAGGA GGAAGTTGGA 
ACTTTCTCAC GGTGTGTAAG TGCTGCCTCG GGCACAGAGA GTGCCGAACG AACGCAGATG 
AGAGACATCT TGCAGCAGCA TCTTGACAAG TCTAAAACAC TTAGAGTCTG CAACCCACTG 
ACTGGCGTGC GGTATGTTCA GGAGAACAGC CCTTGTGTGA CTCCCGTCTC CACAGCAACA 
CACAGCCTGA ACCGTCTTCA CACCATGCTG GCCGGCCTCA GGAATGCACC CAGTGAGAAG 
CTAGAACAGA TACTCAGGTC ATGTTCCCGA GATCCGACTC GGGCTATCGC TGACAGGTTG 
AGAGAGATGT ATGAAATATA TTCTCAGCAT TTCCAGCCAG ACGAGAATGT TAGTAATTGT 
GCTAAAGAAA TGGCCAACAA ACACTTTCGT TTTGCAGAAA TGCTTTACTA TAAAGTATTA 
GAGTCTGTTA TTGAGCAAGA ACAGAAGAGA TTGGGAGACA TGGACTTATC TGGTGTTCTG 
GAGCAAGATG CATTCCATAA GTCACTCCTG GCCTGCTGCC TTGAGGTGGT CGCTTTTTCC 
TATAAGCCTC CTGGAAATTT CCCATTTATT GCTGAAATAT TTGATGTACC ACATTATCAT 
TTTTATAAGG TAATAGAAGT ATTTATTAGA GCAGAAGACG GTCTTTGCAG AGAAGTGGTC 
AAACACCTCA ATCAGATTGA AGAACAAATT TTAGACCACT TGGCATGGAA AACCAAGTCC 
CCACTGTGGG ATAGAATTAG AGATAATGAA AACAGAGTCC CTACATGTGA AGAGGTTACA 
CCACCTCACA ACCTAGAGAG AACAGATGAA ATCTATATTG CTGGCTCTCC CTTAACTCCG 
AGAAGGGTGG GTGAAGTTCG CACTGATGCT GGAGGACTTG GAAGAAGTGT GACATCTCCA 
ACCACACTGT ATGACAGGTA CAGTTCCCCA ACAGTCAGTA CTACTAGAAG GCGGCTATTT 
GAGAGTGACA GTCCCTCTGA AGGAAGCACG GCTGGGCGCA TCCCCCCACA ACCCCTAGTC 
AATGCTGTCC CTGTGCAGAA TGTATCTGGG GAGACTGTTT CTGTCACACC AGTTCCTGGA 
CAGACCTTGG TCACCATGGC AACAGCCACT GTCACAGCCA ACAATGGACA AACAGTGACC 
ATTCCAGTAC AAGGTATTGC CAACGAAAAT GGAGGGATAA CATTCTTCCC AGTCCAAGTG 
AATGTTGGGG GCCAGGCACA GGCTGTGACT GGCTCTATCC AGCCCCTCAG TGCTCAAGCG 
CTGGCTGGAA GTCTGAGTTC CCAACAGGTG ACAGGAACAA CTTTGCAAGT CCCCGGTCCG 
GTGGCCATTC AACAAATTTC CCCTGGAGGA CAACAGCAGA ACCAAGGCCA GCCGTTAACC 
AGCAGCAGTA TCAGGCCTCG GAAGACTAGC TCCTTATCAC TTTTCTTTAG AAAGGTTTAC 
TACTTAGCTG GTGTCCGCCT TCGAGACCTT TGTACAAAAC TAGATATTTC AGATGAACTG 
AGGAAAAAAA TTTGGACCTG CTTTGAATTC TCTATAGTCC AGTGTCCTGA ACTTATGATG 
GACAGACATC TGGACCAGTT GTTAATGTGT GCCATTTACG TGATGGCAAA GGTCACAAAA 
GAAGACAAGT CCTTCCAGAA TATCATGCGC TGTTACAGGA CTCAGCCACA GGCCCGGAGC 
CAGGTATACA GAAGTGTCTT GATAAAAGGG AAAAGAAGAA ATTCTGGCAG CTGTGAGAAC 
AGAAGCCATC AGAATTCTCC AACAGAACTA AATACAGACA GAGCCAGTAG AGACTCCAGT 
CCAGTTATGA GGTCAAACAG CACCTTACCA GTTCCACAGC CCAGCAGTGC CCCTCCTACA 
CCAACTCGAC TCACAGGTGC CAACAGTGAC ATTGAAGAGG AGGAAAGAGG AGACCTCATT 
CAGTTCTACA ACAACATCTA CAGGAAACAA ATCCAAACGT TTGCCATGAA GTACTCACAG 
GCAAACTCCC AAATGGACAC CCCTCCCCTG TCTCCCTATC CGTTTGTAAG AACGGGCTCC 
CCTCGCCGAG TACAGTTATC TCAAAGTCAC CCTATCTACA TTTCCCCACA TAAAAACGAA 
GCAATGCTTT CTCCTCGAGA GAAGATTTTT TACTACTTCA GCAACAGCCC ATCAAAGAGA 
CTGAGAGAAA TTAACAGTAT GATACGGACA GGAGAGACTC CAACTAAAAA GAGAGGGATT 
CTCTTGGACG ATGGAAGTGA ATCGCCTGCA AAAAGAATTT GCCCAGAAAA TCATTCTGCT 
CTGTTACGTC GTCTCCAAGA TGTAGCTAAT GACCGAGGTT CACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rbl2 ( XM_006255199.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255261.1 Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), transcript variant X1, mRNA.

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CloneID	ORa38663
Clone ID Related Accession (Same CDS sequence)	XM_006255199.3
Accession Version	XM_006255199.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	retinoblastoma-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006255199.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTG GAGGCAACCA GTCGTCACCT CCCCCTCCTG CTGCTGCAGC CAGCTCAGAG 
GAAGAGGAGG AGGATGGCGA CACCGCGGAC CGGGCGCAGC CCGCAGGGTC CCCAAGCCAT 
CAGATCCAAC AGCGGTTCGA AGAGCTGTGC AGCCGCCTTA ACATGGACGA GGCGGCACGC 
GCCGAGGCCT GGAGCAGCTA CCGCAGCATG AGCGAGAGCT ACACGCTGGA GGGAAATGAT 
CTCCATTGGT TAGCATGTGC CTTATATGTG GCTTGCAGAA AATCTGTTCC TACTGTGAGC 
AAAGGGACTG CTGAAGGAAA CTATGTGTCT TTAACTAGAA TCCTTCGATG TTCTGAGCAA 
AGCCTAATTG AATTTTTTAA CAAGATGAAG AAGTGGGAAG ATATGGCAAA TTTACCCCCA 
CACTTCCGAG AACGAACCGA AAGATTAGAA AGAAACTTCA CTGTCTCCGC TGTGATCTTT 
AAGAAATATG AACCCATTTT TCAAGACATT TTTAAATATC CCCAAGAAGA ACAACCTCGC 
CAGCAAAGAG GAAGAAAGCA GAGGCGACAG CCCTGTACCA CATCTGAAAT TTTCCATTTT 
TGTTGGGTGC TTTTTATATA CGCAAAAGGA AACTTCCCCA TGATCAGTGA TGATCTGGTC 
AATTCCTACC ATCTTCTGCT GTGTGCACTA GATTTAGTTT ATGGAAATGC CCTACAATGC 
TCTAACCGGA AAGAACTTGT GAACCCTAAT TTTAAAGGCG TGTCCGAGGA CGGCCACCCC 
AGGGACTCTC ACCCGTCCTC TGATCCACCG TGTGTCATTG AGAAGCTCTG CTCCTTACAC 
GACGGCCTAG TGCTGGAGGC AAAGGGGATA AAGCAGCATT TCTGGAAACC CTACATTAGG 
AAGCTTTTTG AAAAGAAGCT CCTCAGGGGG AAGGAAGAAA ATCTCACTGG CTTCCTGGAG 
CCCGGGAACT TCGCAGAGAG CTTTAAGGCT GTTAATAAGG CGTATGAAGA ATACGTGTTA 
GCCACTGGGA GTCTGGATGA GCGGATATTT CTTGGCGAGG ATGCGGAGGA GGAAGTTGGA 
ACTCTCTCAC GGTGTGTAAG TGCTGCCTCG GGCACAGAGA GTGCCGAACG AACGCAGATG 
AGAGACATCT TGCAGCAGCA TCTTGACAAG TCTAAAACAC TTAGAGTCTG CAACCCACTG 
ACTGGCGTGC GGTATGTTCA GGAGAACAGC CCTTGTGTGA CTCCCGTCTC CACAGCAACA 
CACAGCCTGA ACCGTCTTCA CACCATGCTG GCCGGCCTCA GGAATGCACC CAGTGAGAAG 
CTAGAACAGA TACTCAGGTC ATGTTCCCGA GATCCGACTC GGGCTATCGC TGACAGGTTG 
AGAGAGATGT ATGAAATATA TTCTCAGCAT TTCCAGCCAG ACGAGAATGT TAGTAATTGT 
GCTAAAGAAA TGGCCAACAA ACACTTTCGT TTTGCAGAAA TGCTTTACTA TAAAGTATTA 
GAGTCTGTTA TTGAGCAAGA ACAGAAGAGA TTGGGAGACA TGGACTTATC TGGTGTTCTG 
GAGCAAGATG CATTCCATAA GTCACTCCTG GCCTGCTGCC TTGAGGTGGT CGCTTTTTCC 
TATAAGCCTC CTGGAAATTT CCCATTTATT GCTGAAATAT TTGATGTACC ACATTATCAT 
TTTTATAAGG TAATAGAAGT ATTTATTAGA GCAGAAGACG GTCTTTGCAG AGAAGTGGTC 
AAACACCTCA ATCAGATTGA AGAACAAATT TTAGACCACT TGGCATGGAA AACCAAGTCC 
CCACTGTGGG ATAGAATTAG AGATAATGAA AACAGAGTCC CTACATGTGA AGAGGTTACA 
CCACCTCACA ACCTAGAGAG AACAGATGAA ATCTATATTG CTGGCTCTCC CTTAACTCCG 
AGAAGGGTGG GTGAAGTTCG CACTGATGCT GGAGGACTTG GAAGAAGTGT GACATCTCCA 
ACCACATTGT ATGACAGGTA CAGTTCCCCA ACAGTCAGTA CTACTAGAAG GCGGCTATTT 
GAGAGTGACA GTCCCTCTGA AGGAAGCACG GCTGGGCGCA TCCCCCCACA ACCCCTAGTC 
AATGCTGTCC CTGTGCAGAA TGTATCTGGG GAGACTGTTT CTGTCACACC AGTTCCTGGA 
CAGACCTTGG TCACCATGGC AACAGCCACT GTCACAGCCA ACAATGGACA AACAGTGACC 
ATTCCAGTAC AAGGTATTGC CAACGAAAAT GGAGGGATAA CATTCTTCCC AGTCCAAGTG 
AATGTTGGGG GCCAGGCACA GGCTGTGACT GGCTCTATCC AGCCCCTCAG TGCTCAAGCG 
CTGGCTGGAA GTCTGAGTTC CCAACAGGTG ACAGGAACAA CTTTGCAAGT CCCCGGTCCG 
GTGGCCATTC AACAAATTTC CCCTGGAGGA CAACAGCAGA ACCAAGGCCA GCCGTTAACC 
AGCAGCAGTA TCAGGCCTCG GAAGACTAGC TCCTTATCAC TTTTCTTTAG AAAGGTTTAC 
TACTTAGCTG GTGTCCGCCT TCGAGACCTT TGCACAAAAC TAGATATTTC AGATGAACTG 
AGGAAAAAAA TTTGGACCTG CTTTGAATTC TCTATAGTCC AGTGTCCTGA ACTTATGATG 
GACAGACATC TGGACCAGTT GTTAATGTGT GCCATTTACG TGATGGCAAA GGTCACAAAA 
GAAGACAAGT CCTTCCAGAA TATCATGCGC TGTTACAGGA CTCAGCCACA GGCCCGGAGC 
CAGGTATACA GAAGTGTCTT GATAAAAGGG AAAAGAAGAA ATTCTGGCAG CTGTGAGAAC 
AGAAGCCATC AGAATTCTCC AACAGAACTA AATACAGACA GAGCCAGTAG AGACTCCAGT 
CCAGTTATGA GGTCAAACAG CACCTTACCA GTTCCACAGC CCAGCAGTGC CCCTCCTACA 
CCAACTCGAC TCACAGGTGC CAACAGTGAC ATTGAAGAGG AGGAAAGAGG AGACCTCATT 
CAGTTCTACA ACAACATCTA CAGGAAACAA ATCCAAACGT TTGCCATGAA GTACTCACAG 
GCAAACTCCC AACAGATGGA CACCCCTCCC CTGTCTCCCT ATCCGTTTGT AAGAACGGGC 
TCCCCTCGCC GAGTACAGTT ATCTCAAAGT CACCCTATCT ACATTTCCCC ACATAAAAAC 
GAAGCAATGC TTTCTCCTCG AGAGAAGATT TTTTACTACT TCAGCAACAG CCCATCAAAG 
AGACTGAGAG AAATTAACAG TATGATACGG ACAGGAGAGA CTCCAACTAA AAAGAGAGGG 
ATTCTCTTGG ACGATGGAAG TGAATCGCCT GCAAAAAGAA TTTGCCCAGA AAATCATTCT 
GCTCTGTTAC GTCGTCTCCA AGATGTAGCT AATGACCGAG GTTCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255261.1
CDS	122..3532
Translation	MASGGNQSSPPPPAAAASSEEEEEDGDTADRAQPAGSPSHQIQQRFEELCSRLNMDEAARAEAWSSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTAEGNYVSLTRILRCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTTSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGVSEDGHPRDSHPSSDPPCVIEKLCSLHDGLVLEAKGIKQHFWKPYIRKLFEKKLLRGKEENLTGFLEPGNFAESFKAVNKAYEEYVLATGSLDERIFLGEDAEEEVGTLSRCVSAASGTESAERTQMRDILQQHLDKSKTLRVCNPLTGVRYVQENSPCVTPVSTATHSLNRLHTMLAGLRNAPSEKLEQILRSCSRDPTRAIADRLREMYEIYSQHFQPDENVSNCAKEMANKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGVLEQDAFHKSLLACCLEVVAFSYKPPGNFPFIAEIFDVPHYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKTKSPLWDRIRDNENRVPTCEEVTPPHNLERTDEIYIAGSPLTPRRVGEVRTDAGGLGRSVTSPTTLYDRYSSPTVSTTRRRLFESDSPSEGSTAGRIPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGPVAIQQISPGGQQQNQGQPLTSSSIRPRKTSSLSLFFRKVYYLAGVRLRDLCTKLDISDELRKKIWTCFEFSIVQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRRNSGSCENRSHQNSPTELNTDRASRDSSPVMRSNSTLPVPQPSSAPPTPTRLTGANSDIEEEERGDLIQFYNNIYRKQIQTFAMKYSQANSQQMDTPPLSPYPFVRTGSPRRVQLSQSHPIYISPHKNEAMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLDDGSESPAKRICPENHSALLRRLQDVANDRGSH

Target ORF information:

RefSeq Version	XM_006255199.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RB transcriptional corepressor like 2 (Rbl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255199.3

ORF Insert Sequence:

ATGGCATCTG GAGGCAACCA GTCGTCACCT CCCCCTCCTG CTGCTGCAGC CAGCTCAGAG 
GAAGAGGAGG AGGATGGCGA CACCGCGGAC CGGGCGCAGC CCGCAGGGTC CCCAAGCCAT 
CAGATCCAAC AGCGGTTCGA AGAGCTGTGC AGCCGCCTTA ACATGGACGA GGCGGCACGC 
GCCGAGGCCT GGAGCAGCTA CCGCAGCATG AGCGAGAGCT ACACGCTGGA GGGAAATGAT 
CTCCATTGGT TAGCATGTGC CTTATATGTG GCTTGCAGAA AATCTGTTCC TACTGTGAGC 
AAAGGGACTG CTGAAGGAAA CTATGTGTCT TTAACTAGAA TCCTTCGATG TTCTGAGCAA 
AGCCTAATTG AATTTTTTAA CAAGATGAAG AAGTGGGAAG ATATGGCAAA TTTACCCCCA 
CACTTCCGAG AACGAACCGA AAGATTAGAA AGAAACTTCA CTGTCTCCGC TGTGATCTTT 
AAGAAATATG AACCCATTTT TCAAGACATT TTTAAATATC CCCAAGAAGA ACAACCTCGC 
CAGCAAAGAG GAAGAAAGCA GAGGCGACAG CCCTGTACCA CATCTGAAAT TTTCCATTTT 
TGTTGGGTGC TTTTTATATA CGCAAAAGGA AACTTCCCCA TGATCAGTGA TGATCTGGTC 
AATTCCTACC ATCTTCTGCT GTGTGCACTA GATTTAGTTT ATGGAAATGC CCTACAATGC 
TCTAACCGGA AAGAACTTGT GAACCCTAAT TTTAAAGGCG TGTCCGAGGA CGGCCACCCC 
AGGGACTCTC ACCCGTCCTC TGATCCACCG TGTGTCATTG AGAAGCTCTG CTCCTTACAC 
GACGGCCTAG TGCTGGAGGC AAAGGGGATA AAGCAGCATT TCTGGAAACC CTACATTAGG 
AAGCTTTTTG AAAAGAAGCT CCTCAGGGGG AAGGAAGAAA ATCTCACTGG CTTCCTGGAG 
CCCGGGAACT TCGCAGAGAG CTTTAAGGCT GTTAATAAGG CGTATGAAGA ATACGTGTTA 
GCCACTGGGA GTCTGGATGA GCGGATATTT CTTGGCGAGG ATGCGGAGGA GGAAGTTGGA 
ACTCTCTCAC GGTGTGTAAG TGCTGCCTCG GGCACAGAGA GTGCCGAACG AACGCAGATG 
AGAGACATCT TGCAGCAGCA TCTTGACAAG TCTAAAACAC TTAGAGTCTG CAACCCACTG 
ACTGGCGTGC GGTATGTTCA GGAGAACAGC CCTTGTGTGA CTCCCGTCTC CACAGCAACA 
CACAGCCTGA ACCGTCTTCA CACCATGCTG GCCGGCCTCA GGAATGCACC CAGTGAGAAG 
CTAGAACAGA TACTCAGGTC ATGTTCCCGA GATCCGACTC GGGCTATCGC TGACAGGTTG 
AGAGAGATGT ATGAAATATA TTCTCAGCAT TTCCAGCCAG ACGAGAATGT TAGTAATTGT 
GCTAAAGAAA TGGCCAACAA ACACTTTCGT TTTGCAGAAA TGCTTTACTA TAAAGTATTA 
GAGTCTGTTA TTGAGCAAGA ACAGAAGAGA TTGGGAGACA TGGACTTATC TGGTGTTCTG 
GAGCAAGATG CATTCCATAA GTCACTCCTG GCCTGCTGCC TTGAGGTGGT CGCTTTTTCC 
TATAAGCCTC CTGGAAATTT CCCATTTATT GCTGAAATAT TTGATGTACC ACATTATCAT 
TTTTATAAGG TAATAGAAGT ATTTATTAGA GCAGAAGACG GTCTTTGCAG AGAAGTGGTC 
AAACACCTCA ATCAGATTGA AGAACAAATT TTAGACCACT TGGCATGGAA AACCAAGTCC 
CCACTGTGGG ATAGAATTAG AGATAATGAA AACAGAGTCC CTACATGTGA AGAGGTTACA 
CCACCTCACA ACCTAGAGAG AACAGATGAA ATCTATATTG CTGGCTCTCC CTTAACTCCG 
AGAAGGGTGG GTGAAGTTCG CACTGATGCT GGAGGACTTG GAAGAAGTGT GACATCTCCA 
ACCACATTGT ATGACAGGTA CAGTTCCCCA ACAGTCAGTA CTACTAGAAG GCGGCTATTT 
GAGAGTGACA GTCCCTCTGA AGGAAGCACG GCTGGGCGCA TCCCCCCACA ACCCCTAGTC 
AATGCTGTCC CTGTGCAGAA TGTATCTGGG GAGACTGTTT CTGTCACACC AGTTCCTGGA 
CAGACCTTGG TCACCATGGC AACAGCCACT GTCACAGCCA ACAATGGACA AACAGTGACC 
ATTCCAGTAC AAGGTATTGC CAACGAAAAT GGAGGGATAA CATTCTTCCC AGTCCAAGTG 
AATGTTGGGG GCCAGGCACA GGCTGTGACT GGCTCTATCC AGCCCCTCAG TGCTCAAGCG 
CTGGCTGGAA GTCTGAGTTC CCAACAGGTG ACAGGAACAA CTTTGCAAGT CCCCGGTCCG 
GTGGCCATTC AACAAATTTC CCCTGGAGGA CAACAGCAGA ACCAAGGCCA GCCGTTAACC 
AGCAGCAGTA TCAGGCCTCG GAAGACTAGC TCCTTATCAC TTTTCTTTAG AAAGGTTTAC 
TACTTAGCTG GTGTCCGCCT TCGAGACCTT TGCACAAAAC TAGATATTTC AGATGAACTG 
AGGAAAAAAA TTTGGACCTG CTTTGAATTC TCTATAGTCC AGTGTCCTGA ACTTATGATG 
GACAGACATC TGGACCAGTT GTTAATGTGT GCCATTTACG TGATGGCAAA GGTCACAAAA 
GAAGACAAGT CCTTCCAGAA TATCATGCGC TGTTACAGGA CTCAGCCACA GGCCCGGAGC 
CAGGTATACA GAAGTGTCTT GATAAAAGGG AAAAGAAGAA ATTCTGGCAG CTGTGAGAAC 
AGAAGCCATC AGAATTCTCC AACAGAACTA AATACAGACA GAGCCAGTAG AGACTCCAGT 
CCAGTTATGA GGTCAAACAG CACCTTACCA GTTCCACAGC CCAGCAGTGC CCCTCCTACA 
CCAACTCGAC TCACAGGTGC CAACAGTGAC ATTGAAGAGG AGGAAAGAGG AGACCTCATT 
CAGTTCTACA ACAACATCTA CAGGAAACAA ATCCAAACGT TTGCCATGAA GTACTCACAG 
GCAAACTCCC AACAGATGGA CACCCCTCCC CTGTCTCCCT ATCCGTTTGT AAGAACGGGC 
TCCCCTCGCC GAGTACAGTT ATCTCAAAGT CACCCTATCT ACATTTCCCC ACATAAAAAC 
GAAGCAATGC TTTCTCCTCG AGAGAAGATT TTTTACTACT TCAGCAACAG CCCATCAAAG 
AGACTGAGAG AAATTAACAG TATGATACGG ACAGGAGAGA CTCCAACTAA AAAGAGAGGG 
ATTCTCTTGG ACGATGGAAG TGAATCGCCT GCAAAAAGAA TTTGCCCAGA AAATCATTCT 
GCTCTGTTAC GTCGTCTCCA AGATGTAGCT AATGACCGAG GTTCACACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Post-transcriptional gene expression control by NANOS is up-regulated and functionally important in pRb-deficient cells.
The EMBO journal33(19)2201-15(2014 Oct)
Miles WO,Korenjak M,Griffiths LM,Dyer MA,Provero P,Dyson NJ

The degradation of cell cycle regulators by SKP2/CKS1 ubiquitin ligase is genetically controlled in rodent liver cancer and contributes to determine the susceptibility to the disease.
International journal of cancer126(5)1275-81(2010 Mar)
Calvisi DF,Pinna F,Ladu S,Muroni MR,Frau M,Demartis I,Tomasi ML,Sini M,Simile MM,Seddaiu MA,Feo F,Pascale RM

In vitro senescence of rat mesenchymal stem cells is accompanied by downregulation of stemness-related and DNA damage repair genes.
Stem cells and development18(7)1033-42(2009 Sep)
Galderisi U,Helmbold H,Squillaro T,Alessio N,Komm N,Khadang B,Cipollaro M,Bohn W,Giordano A

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

The PPARalpha/p16INK4a pathway inhibits vascular smooth muscle cell proliferation by repressing cell cycle-dependent telomerase activation.
Circulation research103(10)1155-63(2008 Nov)
Gizard F,Nomiyama T,Zhao Y,Findeisen HM,Heywood EB,Jones KL,Staels B,Bruemmer D

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Rbl2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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