Green PCR Reagents

Products

Green Taq DNA Polymerase

Green Taq DNA Polymerase has terminal transferase activity that results in the addition of single nucleotide (adenosine) at 3'-end of the extension product. This enzyme also has a longer enzyme half-life and therefore increases the PCR yield when amplifying long DNA fragments.

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High-Stability PCR Kit

GenScript's High-Stability PCR Kit contains Green Taq DNA Polymerase, 10X PCR buffer, and 10 mM dNTP, all specially formulated for higher stability using our proprietary technology. The stabilizers in the storage buffer also increase the stability of Taq DNA Polymerase at higher temperatures (72°C), increasing the PCR yield of long DNA amplification. This Kit makes the PCR step quick and easy by eliminating any need for thawing. This kit can be used in high-throughput assays in which all the PCR reagents need to be ready and stable for PCR at any time.

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Examples

• Examples

  Specification of High-Stability PCR Kit

  Components	Volume	Documents
  Green Taq DNA polymerase	100 μl	DNA Polymerase PDF
  10X reaction buffer	1.5 ml
  Stabilized dNTP	300 μl
  Protocol	1

  • The Green Taq DNA polymerase provides high PCR yield and specificity when amplifying long DNA fragments.
    
  
  
  

  Figure 1. An 8-kb fragment from λDNA was amplified by using both Taq DNA polymeraseand Green Taq DNA polymerase. For long DNA fragment amplification, where longer elongation time is needed (In this case, the elongation time was eight minutes.), the Green Taq DNA polymerase has a longer half-life and therefore increases the PCR DNA yields significantly.

  • The Green Taq DNA polymerase can be stored at 37°C for 14 days without activity loss.
    

  Figure 2. A 2-kb 23S rRNA fragment from from E. coli genomic DNA was amplified using GreenTaq DNA polymerase stored at -20°C and 37°C, respectively.

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  Cat. No.	Product Name	Quantity	Price	Order
  A01849	Hot Start Taq Antibody		$126.00 250 U (5U/μl) 1000 U (5U/μl)
  C01577	dATP (100 mM) ≥99% HPLC		$52.00 1 ml(1.0ml/vial) 10 ml (10.0ml/vial) 50 ml (50ml/bottle) 250 ml (250.0ml/bottle)
  C01578	dGTP (100 mM) ≥99% HPLC		$52.00 1 ml(1.0ml/vial) 10 ml (10.0ml/vial) 50 ml (50ml/bottle) 250 ml (250.0ml/bottle)
  C01579	dCTP (100 mM) ≥99% HPLC		$52.00 1 ml(1.0ml/vial) 10 ml (10.0ml/vial) 50 ml (50ml/bottle) 250 ml (250.0ml/bottle)
  C01580	dTTP (100 mM) ≥99% HPLC		$52.00 1 ml(1.0ml/vial) 10 ml (10.0ml/vial) 50 ml (50ml/bottle) 250 ml (250.0ml/bottle)
  C01582	dNTP (10 mM each) ≥99% HPLC		$52.00 1 ml(1.0ml/vial) 10 ml (10.0ml/vial) 50 ml (50ml/bottle) 250 ml (250.0ml/bottle)
  C01689	Stabilized dNTP Mix, 10 mM each		$34.00 0.5 ml
  C01717	dUTP (100 mM) ≥99% HPLC		$63.00 1 ml(1.0ml/vial) 10 ml(10.0ml/vial) 50 ml (50ml/bottle) 250 ml(250.0ml/bottle)
  D0056	dNTP mixture, 10 mM each		$29.00 0.5 ml
  E00007	Taq DNA Polymerase		$66.00 1000 U (1000.0U/vial) 50000 U (1000.0U/vial)
  E00008	Taq DNA Polymerase without Mg2+		$66.00 1000 U (1000.0U/vial)
  E00019	2X Taq Master Mix		$48.00 100 Reactions (2.5 mL)
  E00101	Taq DNA Polymerase (1000 U) with 10 mM dNTP Mix (0.5 ml)		$86.00 1 PK