Rbf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rbf
Entrez Gene ID	31027
Full Name	Retinoblastoma-family protein
Synonyms	CG7413,Dmel\CG7413,EG:34F3.3,FBF,RB,RBF,RBF1,RBf1,Rb,Rb1,RbF,Rbf1,dRBF,pRb,rb,rbf,rbf1
General protein information	Preferred Names Retinoblastoma-family protein Names CG7413 gene product from transcript CG7413-RA CG7413-PA Rb Rbf-PA retinoblastoma retinoblastoma factor retinoblastoma factor-1 retinoblastoma family protein 1 retinoblastoma-family homolog
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1C5-1C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_080297.3	NP_525036.2	Retinoblastoma-family protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RBL2	XP_523371.2
Canis lupus familiaris (dog)	RBL2	XP_535303.2
Danio rerio (zebrafish)	rbl2	XP_002667000.3
Macaca mulatta (Rhesus monkey)	RBL2	XP_002802522.1
Drosophila melanogaster (fruit fly)	Rbf	NP_525036.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006477	XP_316517.4
Bos taurus (cattle)	RBL2	NP_001091542.1
Rattus norvegicus (Norway rat)	Rbl2	NP_112356.1
Homo sapiens (human)	RBL2	NP_005602.3
Mus musculus (house mouse)	Rbl2	NP_035380.3
Xenopus tropicalis (tropical clawed frog)	rbl2	XP_002937369.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rbf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rbf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00405	NM_080297.3 Latest version!	Drosophila melanogaster Retinoblastoma-family protein (Rbf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rbf ( NM_080297.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525036.2 Drosophila melanogaster Retinoblastoma-family protein (Rbf), mRNA.

Price & Availability↑

CloneID	OFa00405
Clone ID Related Accession (Same CDS sequence)	NM_080297.3
Accession Version	NM_080297.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556035200000
Organism	Drosophila melanogaster(fruit fly)
Product	Retinoblastoma-family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080297.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGAGC CTGACCCGCA GGAACTGGGA GCTGAGGTGG TCAGCGGATT GGTGGCGACC 
AGCGACGACC GCCTGGAGAT GATCAACGCC GAGTACACGA ACCTATGCCG GGATCTAAAC 
ATGGATCGGC AGACAGAGCT GCAGGGCTAC GAGACGTACT TAGAGGTGTC CCAGAGGTGC 
TCCATGGAGG GCACAGCCAG CCACTGGATG TGCTGCGCCA TTTACACGGC CTGCCGAAGA 
ACCAGCACAC CCACGGTGAC TGGCCAGAAT GCAGTGGTCA AGGGTAATTG TGTGTCCTTA 
AACAATTTGC TGCGCTGCTG CAAGATGAGC ATCTACGAGT TCAAGACCAA AATTAAGCAG 
TGGTGCGACA TGGCCAACCT GCCTCAGGAG TTTGTCAACG AGATTGAGGA TCTGGACCGC 
AAGTTCAGCA TTACATTCAT GCTGCACAAG CGTTTCCGCA TTATTATGGA CATGATCTTC 
TCGTGCCCGC CCAACGAGAA GAAGCACTCC AAGTACATTT CTCTGCATGG CAACCACGCC 
CACGGAAAGT GCTCGTACAT CAAGCTGGAC GACATCTGCT GGCGCCTGTT TCTCTGCGCC 
AAGAACCAGA AGCCGAGCAA CACCGTGGAC CTGGTGACAT CCTACAATCT GATGATTTGC 
TGCATAGACC TGATATACAA CAATGTGCTG GCCGAGAAGC GCACCGATCT CATCAACCCC 
AAATTCGAGG GCCTGCCGAG CAACTGGACG GAGCTGGACT TTCGTCACAA TCCGCACTGC 
ATTTTGTCCA ACTTCTGTGA CATGACCGAG GAGGCTAAAG CCATGAAGGC CACCACTTTC 
CGCCAGATCA TGTCGAGCTT CTTTCAGGCT TCGACCATCT ACGGCAACAA GGACACTATG 
TTAGGCCTAT TGGCTAACGA AAACTTCGAG CGCAACCTAA AGTCCCTCAA CATCAGCTAC 
GAGCAGTACG TCCTCAGCGT CGGCGAATTC GACGAACGCA TTCTGAGTGC CTACGATGCG 
GGGGAACACA CTGCCCTCAA CGACCAGTCC CTGCGTCCGC CGGTTACTCC GCTTACCCGC 
AAGCAAGATC TGCCTGCGCA GCCCGCGATG GCTGGCGATA AATTTGAGCC CGTCCGCAAC 
GCCACTAACA ACGTGAAGCA GCTGAGCGCC TTCGGACGGA TCACAGAACC CACGGACTTT 
GTCAAGCAAG CTGGCGAAGA GGTAATAGCC AAGCTGCTTA GCATCATCGA GGAGATCGAG 
CAGAAATTCC TCGCCAAGTA CCCGTCAACG GAGGCGAAGA GTCGCTTCCA ACTAGCGAAG 
TCCTTCTTCT TCTACCTGCT CGACCAGATC CTGCAGGCGG AAATTCGGAA CAAGCCCGAC 
ATCGATCTCA AGCGACTGCT GGTGCAGAAG GTCTCCCTGG TCATCTTTAA CATCACGCTG 
ATGGCCTGCT GCGTGGAGCT GGTGCTGGAG GCCTACAAAA CGGAGCTCAA GTTCCCGTGG 
GTGCTCGACT GTTTCAGTAT TAGCGCGTTC GAGTTCCAAA AGATCATCGA GATCGTGGTG 
CGGCACGGCT CGCACGAAGG CTGTTTGAAC CGGTCGCTGA TAAAGCACCT TAACTCCATC 
GAGGAGACGT GTCTGGAGCG GCTGGCCTGG GCGAGGAACT CGACCGTCTG GGAAATGATC 
GCCTCCGCCC AACTGCCGTT GCCCACGTGG CTCATGGTTA ACCTCGACCG CGCCGCCGGC 
CCCTTGCAGA TATTCCTGCG CAAGGTGTAC CTGCTTGGCT GGTTGCGCAT CCAGAAGCTG 
TGCTCGGAGC TGTCACTGTG CGAGAAGACG CCCGAGAGCA TCTGGCACAT CTTTGAGCAC 
TCGATCACCC ACGAGACGGA ATTGATGAAG GACCGCCACC TCGATCAAAA TATTATGTGT 
GCAATATATA TCTACATAAG AGTAAAGCGA ATGGAGGACC CCAAGTTCAG TGACATTATG 
CGGGCATATC GCAACCAGCC GCAGGCGGTG AACAGCGTCT ACCGCGAGGT GTTCATCGAC 
ATCAACGAGG ACGGGGAGCC AAAGGTGAAG GACATCATTC ACTTTTACAA CCACACTTAC 
GTGCCGCTGA TGCGGCAGTT CGTCATCGAC TACCTAAACG TAACTCCGGA CGTGAGTGGC 
CGGGCTTCGG ATCTACAGCT CTCCCCGCAT CCGAAGGAAC GGGCCGCCCA GCCCAAGAAG 
GTAACCCAGA GCCACTCGCT GTTCGTGTCG CAGATGTCCA AGAACGAGAT ACAGCAGTCG 
CCCAACCAGA TGGTCTACAG TTTTTGCCGC AGCCCCGCCA AGGACCTTCA GGCTATGAAC 
GAAAAGGTAC GCGGCGGCAA GCGCATGCTG AGCTTTGGCG ACGAGCCCGG ACTAGGTACA 
ATGGCAGAGA CGAAGCGCTC GAAAATCAGC CAGGTCAAGG CCGTCATGGA CGACCCTGAA 
CTGCAGTCAG CCGAGCAGCA GACTGCTGTT ACCACGGAAG GTTGCGTAGG GGGCGAAGGA 
GGAGAACACG AGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525036.2
CDS	695..3232
Translation	MSEPDPQELGAEVVSGLVATSDDRLEMINAEYTNLCRDLNMDRQTELQGYETYLEVSQRCSMEGTASHWMCCAIYTACRRTSTPTVTGQNAVVKGNCVSLNNLLRCCKMSIYEFKTKIKQWCDMANLPQEFVNEIEDLDRKFSITFMLHKRFRIIMDMIFSCPPNEKKHSKYISLHGNHAHGKCSYIKLDDICWRLFLCAKNQKPSNTVDLVTSYNLMICCIDLIYNNVLAEKRTDLINPKFEGLPSNWTELDFRHNPHCILSNFCDMTEEAKAMKATTFRQIMSSFFQASTIYGNKDTMLGLLANENFERNLKSLNISYEQYVLSVGEFDERILSAYDAGEHTALNDQSLRPPVTPLTRKQDLPAQPAMAGDKFEPVRNATNNVKQLSAFGRITEPTDFVKQAGEEVIAKLLSIIEEIEQKFLAKYPSTEAKSRFQLAKSFFFYLLDQILQAEIRNKPDIDLKRLLVQKVSLVIFNITLMACCVELVLEAYKTELKFPWVLDCFSISAFEFQKIIEIVVRHGSHEGCLNRSLIKHLNSIEETCLERLAWARNSTVWEMIASAQLPLPTWLMVNLDRAAGPLQIFLRKVYLLGWLRIQKLCSELSLCEKTPESIWHIFEHSITHETELMKDRHLDQNIMCAIYIYIRVKRMEDPKFSDIMRAYRNQPQAVNSVYREVFIDINEDGEPKVKDIIHFYNHTYVPLMRQFVIDYLNVTPDVSGRASDLQLSPHPKERAAQPKKVTQSHSLFVSQMSKNEIQQSPNQMVYSFCRSPAKDLQAMNEKVRGGKRMLSFGDEPGLGTMAETKRSKISQVKAVMDDPELQSAEQQTAVTTEGCVGGEGGEHET

Target ORF information:

RefSeq Version	NM_080297.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Retinoblastoma-family protein (Rbf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080297.3

ORF Insert Sequence:

ATGAGCGAGC CTGACCCGCA GGAACTGGGA GCTGAGGTGG TCAGCGGATT GGTGGCGACC 
AGCGACGACC GCCTGGAGAT GATCAACGCC GAGTACACGA ACCTATGCCG GGATCTAAAC 
ATGGATCGGC AGACAGAGCT GCAGGGCTAC GAGACGTACT TAGAGGTGTC CCAGAGGTGC 
TCCATGGAGG GCACAGCCAG CCACTGGATG TGCTGCGCCA TTTACACGGC CTGCCGAAGA 
ACCAGCACAC CCACGGTGAC TGGCCAGAAT GCAGTGGTCA AGGGTAATTG TGTGTCCTTA 
AACAATTTGC TGCGCTGCTG CAAGATGAGC ATCTACGAGT TCAAGACCAA AATTAAGCAG 
TGGTGCGACA TGGCCAACCT GCCTCAGGAG TTTGTCAACG AGATTGAGGA TCTGGACCGC 
AAGTTCAGCA TTACATTCAT GCTGCACAAG CGTTTCCGCA TTATTATGGA CATGATCTTC 
TCGTGCCCGC CCAACGAGAA GAAGCACTCC AAGTACATTT CTCTGCATGG CAACCACGCC 
CACGGAAAGT GCTCGTACAT CAAGCTGGAC GACATCTGCT GGCGCCTGTT TCTCTGCGCC 
AAGAACCAGA AGCCGAGCAA CACCGTGGAC CTGGTGACAT CCTACAATCT GATGATTTGC 
TGCATAGACC TGATATACAA CAATGTGCTG GCCGAGAAGC GCACCGATCT CATCAACCCC 
AAATTCGAGG GCCTGCCGAG CAACTGGACG GAGCTGGACT TTCGTCACAA TCCGCACTGC 
ATTTTGTCCA ACTTCTGTGA CATGACCGAG GAGGCTAAAG CCATGAAGGC CACCACTTTC 
CGCCAGATCA TGTCGAGCTT CTTTCAGGCT TCGACCATCT ACGGCAACAA GGACACTATG 
TTAGGCCTAT TGGCTAACGA AAACTTCGAG CGCAACCTAA AGTCCCTCAA CATCAGCTAC 
GAGCAGTACG TCCTCAGCGT CGGCGAATTC GACGAACGCA TTCTGAGTGC CTACGATGCG 
GGGGAACACA CTGCCCTCAA CGACCAGTCC CTGCGTCCGC CGGTTACTCC GCTTACCCGC 
AAGCAAGATC TGCCTGCGCA GCCCGCGATG GCTGGCGATA AATTTGAGCC CGTCCGCAAC 
GCCACTAACA ACGTGAAGCA GCTGAGCGCC TTCGGACGGA TCACAGAACC CACGGACTTT 
GTCAAGCAAG CTGGCGAAGA GGTAATAGCC AAGCTGCTTA GCATCATCGA GGAGATCGAG 
CAGAAATTCC TCGCCAAGTA CCCGTCAACG GAGGCGAAGA GTCGCTTCCA ACTAGCGAAG 
TCCTTCTTCT TCTACCTGCT CGACCAGATC CTGCAGGCGG AAATTCGGAA CAAGCCCGAC 
ATCGATCTCA AGCGACTGCT GGTGCAGAAG GTCTCCCTGG TCATCTTTAA CATCACGCTG 
ATGGCCTGCT GCGTGGAGCT GGTGCTGGAG GCCTACAAAA CGGAGCTCAA GTTCCCGTGG 
GTGCTCGACT GTTTCAGTAT TAGCGCGTTC GAGTTCCAAA AGATCATCGA GATCGTGGTG 
CGGCACGGCT CGCACGAAGG CTGTTTGAAC CGGTCGCTGA TAAAGCACCT TAACTCCATC 
GAGGAGACGT GTCTGGAGCG GCTGGCCTGG GCGAGGAACT CGACCGTCTG GGAAATGATC 
GCCTCCGCCC AACTGCCGTT GCCCACGTGG CTCATGGTTA ACCTCGACCG CGCCGCCGGC 
CCCTTGCAGA TATTCCTGCG CAAGGTGTAC CTGCTTGGCT GGTTGCGCAT CCAGAAGCTG 
TGCTCGGAGC TGTCACTGTG CGAGAAGACG CCCGAGAGCA TCTGGCACAT CTTTGAGCAC 
TCGATCACCC ACGAGACGGA ATTGATGAAG GACCGCCACC TCGATCAAAA TATTATGTGT 
GCAATATATA TCTACATAAG AGTAAAGCGA ATGGAGGACC CCAAGTTCAG TGACATTATG 
CGGGCATATC GCAACCAGCC GCAGGCGGTG AACAGCGTCT ACCGCGAGGT GTTCATCGAC 
ATCAACGAGG ACGGGGAGCC AAAGGTGAAG GACATCATTC ACTTTTACAA CCACACTTAC 
GTGCCGCTGA TGCGGCAGTT CGTCATCGAC TACCTAAACG TAACTCCGGA CGTGAGTGGC 
CGGGCTTCGG ATCTACAGCT CTCCCCGCAT CCGAAGGAAC GGGCCGCCCA GCCCAAGAAG 
GTAACCCAGA GCCACTCGCT GTTCGTGTCG CAGATGTCCA AGAACGAGAT ACAGCAGTCG 
CCCAACCAGA TGGTCTACAG TTTTTGCCGC AGCCCCGCCA AGGACCTTCA GGCTATGAAC 
GAAAAGGTAC GCGGCGGCAA GCGCATGCTG AGCTTTGGCG ACGAGCCCGG ACTAGGTACA 
ATGGCAGAGA CGAAGCGCTC GAAAATCAGC CAGGTCAAGG CCGTCATGGA CGACCCTGAA 
CTGCAGTCAG CCGAGCAGCA GACTGCTGTT ACCACGGAAG GTTGCGTAGG GGGCGAAGGA 
GGAGAACACG AGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rbf cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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