RBL2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	RBL2
Entrez Gene ID	100331892
Full Name	retinoblastoma-like 2 (p130)
General protein information	Preferred Names retinoblastoma-like 2 (p130) Names retinoblastoma-like protein 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005163190.3	XP_005163247.1	retinoblastoma-like protein 2 isoform X3
XM_009298209.2	XP_009296484.1	retinoblastoma-like protein 2 isoform X3
XM_009298208.3	XP_009296483.1	retinoblastoma-like protein 2 isoform X2
XM_002666954.5	XP_002667000.3	retinoblastoma-like protein 2 isoform X1
XM_002666954.6	XP_002667000.3	retinoblastoma-like protein 2 isoform X1
XM_005163190.4	XP_005163247.1	retinoblastoma-like protein 2 isoform X3
XM_009298208.2	XP_009296483.1	retinoblastoma-like protein 2 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RBL2	XP_523371.2
Canis lupus familiaris (dog)	RBL2	XP_535303.2
Danio rerio (zebrafish)	rbl2	XP_002667000.3
Macaca mulatta (Rhesus monkey)	RBL2	XP_002802522.1
Drosophila melanogaster (fruit fly)	Rbf	NP_525036.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006477	XP_316517.4
Bos taurus (cattle)	RBL2	NP_001091542.1
Rattus norvegicus (Norway rat)	Rbl2	NP_112356.1
Homo sapiens (human)	RBL2	NP_005602.3
Mus musculus (house mouse)	Rbl2	NP_035380.3
Xenopus tropicalis (tropical clawed frog)	rbl2	XP_002937369.2

Gene Ontology

The following RBL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RBL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa29709	XM_005163190.4 Latest version!	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa29709	XM_009298209.2 Latest version!	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa29708	XM_002666954.6 Latest version!	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa49546	XM_009298208.3 Latest version!	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa29708	XM_002666954.5	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa29709	XM_005163190.3	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa49546	XM_009298208.2	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RBL2 ( XM_005163190.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163247.1 Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa29709
Clone ID Related Accession (Same CDS sequence)	XM_005163190.3 , XM_009298209.2 , XM_005163190.4
Accession Version	XM_005163190.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	retinoblastoma-like protein 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGCG ATGACCTGGT GAACTCGTAT CATCTGCTGC TCTGTGCTCT GGATCTGGTT 
TATTGTAACG CGCTGCTGTG CTCGAGCCGG AAAGATCTGC TCAACCCAGC CTTTAAAGGG 
TTGCCTGAGG ACTTCAACAG TAAAGATTAT AAGCCGGGCC CGGGTTCGTT ATGTTTCATA 
GAGCAGCTTT GTGAGCTTCA CGATGGTCTG GTGCTGGAGG CCAAAGGAGC TAAAGAGCAT 
TTCTGGAAAC CCTTCATTAA GAAATTGTTT GAGCGAAAGA TTCTGAAAGG AAAGGAGGAC 
ACGCTGACAG GATTTTTGGA GCCAGTGAAT TTCGGTGACA GTCTGGCGTC TCTGAACCGT 
GTGTATGAAG AACATGTCCT GTCATCCGGT TCTCTGGATG AGCGAATATT TCTGGGAGAT 
GGAGCTAGAG AAGACATCGG CACACCAGGA CCTTACGAAG GGATAGAGAA CCAGGACAGA 
GCGAACAACC CACTGCAGAA CTCGATGACG GCGTCTGCCC TCAGAGTGTC CACTCCATTG 
ACAGGCAGGA GGTACGTGCC GGAGAGCATT GTGGGGACTC CTGTTTCCAG CGCCATGCAG 
AGTGTGGGCC GACTGCACAC CCTGCTGACG GGATTCAAAT ACAGGCCCAG CGTCAAACTC 
AGAGATATAT TTAGATCCTG TGTAAGAGAC CCCACAGAGT CAGTAGCAGC CAGACTGAAG 
GAGATGTCCG AGATTTTCCT TCAGAATTAT GAGGGAACCG GAGACGACAA CAAGAGCCTG 
GCGAGAGATA TCGCAGTGAA GTATTTTTGC TTGGCGGAGG CACTTTACTA TAAATCCCTG 
GAGGTGATCA TCAACCAGGA GAAGAAACGT CTTGGAGACG TGGACCTGTC TGGCGTTCTG 
GAGCAGGATG TGTTTCACCG CTCGCTGATC GCCTGCTGTC TGGAGATCGT CATCTTCTCC 
TATAGACCAC CAGGAGAGTT TCCCAGAGTC CTGCAAATCT TTGACCTCCC CGCTTATCAC 
TTTTACAAGG TGATCGAGGT GTTGGTACGA GCTGAGGAGG GTCTTTTCCG GGAGGTGGTC 
AAACATCTGA ACCATGTCGA GGAGCAGGTG CTGGAGTCTT TGGCATGGAA AGGAAACTCC 
CCGCTGTGGG AGCGAATCAG AGAGGCCAAA AACTGTGTTC CCACCTGCCA GGAGGTAATG 
CCACCTCAAC ACCTAGAGGA CGGTTCAGGA AGCAACGCAA ACACCCCAAG CAGCAAGACT 
GAATTCAGCG GCAACATCGC AGCGGGAGCA GGAAAAGGAG TTTCTCCGTC CCCTACATCT 
CTCCACGACC GCTACAGTTC TCCCCCTACT GGCACAGCAG TTCGTCGTCT TTTTGTGGAT 
GGAGACACGA CGCCAGAACC TGCTCCACCT GTCAAAGTGG CACCGCCGTC TATAGTCAGC 
TCCATCCCAG CAGGACAGAC TGTGGTTACG ATGGCAACAG CTACTGTCAC AGCGAACAAC 
GGCCAGACTG TTACCATACC TGTTCAAGGA ATAGCGAATG AGAGTGGAGG AATCACATTT 
ATCCCAGTGC AAGTGAGTGT GACGAGTCAG GCTGGAGCGA CTCTGCCAAC CCTCAGTGCG 
CAGACTCTGA CAGGCACCCT GACCACTCAG CAGCTCACAA CAACACCTAT GGGCGCCGTG 
CAAAACAACC CCAGCCCTCC CAAACAGGAC AAAAAGACAC CTCAGCAGGG AACACCCACC 
AAACGGGCCC AGAAAACAGG CTCTCTCTGC CTGTTCTTCC GCAAGGTGTA TCATCTAGCT 
AGTGTGCGTC TCAGAGACTT GTGTGCGAAA CTGGACATCA GTGCGGATCT GCGCAGGAAG 
ATCTGGACCT GTTTCGAGTA TTCGCTTGTC CACTGCACCG ACCTCATGAT GGACCGACAT 
CTTGACCAGC TCCTCATGTG CGCCATCTAC GTCATGACTA AGGTGACGAA AGAGGACAAG 
TCGTTCCAGA ACATCATGAA GTGCTACAGA TCTCAGCCTC AGGCCAGCAG CAGTGTGTAC 
AGAAGTGTGC TGATCTCTGG GAGAAAGAGA CGACACTCTG GAAACACTGA AAACACCCAC 
AGACAGAGCT CACCCACTGA GGGAGCACAA GAACAAGCAA GCGGTGAGAG CAGTCCCGTG 
TCCATGCGCT CCAGCAGTAC GCTGCCCATT CCCCAGCCTA GCAGCGCTCC ATCCACCCCG 
ACCCGGGCAC CGGGAGGGCC CCAGGAGCAG GAGGAGGAGC GGGGGGACCT CATCCGCTTC 
TACAACCACG TCTACATCAA ACAGATCAAA CCCTTCGCTC TGCGCTACTC CAGCAACTCG 
CCCAAAAATG GGCAGGCTGA AACTCCTCCG CTCTGTCCGT ATCCGTCGCT GCGCATCGGC 
TCTCCACGCC GCGTCCTCCT ATCCCACAAT CACTCCATCT ACATCTCTCC GCACAAGACC 
GGCAGCCCCA TATCTCCACG AGACAAAATC TTCTACTACG TCAGCTCCAG CCCGTCACAC 
CGGCTCCGAG AGATCAACAC CATGATCCGA ACCGGAGAGA CGCCCACCAA GAAGCGCAGC 
ATCGCTCTGG AGGAAGAGCA GCAGTCTCCT GCTAAAAGAC TGTGTCAGGA GAACCAGACG 
GCGCTGCTCA GACGCCTGCA GGACGTGGCT AATGACCGCA GCTCCTCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163247.1
CDS	111..2804
Translation	MISDDLVNSYHLLLCALDLVYCNALLCSSRKDLLNPAFKGLPEDFNSKDYKPGPGSLCFIEQLCELHDGLVLEAKGAKEHFWKPFIKKLFERKILKGKEDTLTGFLEPVNFGDSLASLNRVYEEHVLSSGSLDERIFLGDGAREDIGTPGPYEGIENQDRANNPLQNSMTASALRVSTPLTGRRYVPESIVGTPVSSAMQSVGRLHTLLTGFKYRPSVKLRDIFRSCVRDPTESVAARLKEMSEIFLQNYEGTGDDNKSLARDIAVKYFCLAEALYYKSLEVIINQEKKRLGDVDLSGVLEQDVFHRSLIACCLEIVIFSYRPPGEFPRVLQIFDLPAYHFYKVIEVLVRAEEGLFREVVKHLNHVEEQVLESLAWKGNSPLWERIREAKNCVPTCQEVMPPQHLEDGSGSNANTPSSKTEFSGNIAAGAGKGVSPSPTSLHDRYSSPPTGTAVRRLFVDGDTTPEPAPPVKVAPPSIVSSIPAGQTVVTMATATVTANNGQTVTIPVQGIANESGGITFIPVQVSVTSQAGATLPTLSAQTLTGTLTTQQLTTTPMGAVQNNPSPPKQDKKTPQQGTPTKRAQKTGSLCLFFRKVYHLASVRLRDLCAKLDISADLRRKIWTCFEYSLVHCTDLMMDRHLDQLLMCAIYVMTKVTKEDKSFQNIMKCYRSQPQASSSVYRSVLISGRKRRHSGNTENTHRQSSPTEGAQEQASGESSPVSMRSSSTLPIPQPSSAPSTPTRAPGGPQEQEEERGDLIRFYNHVYIKQIKPFALRYSSNSPKNGQAETPPLCPYPSLRIGSPRRVLLSHNHSIYISPHKTGSPISPRDKIFYYVSSSPSHRLREINTMIRTGETPTKKRSIALEEEQQSPAKRLCQENQTALLRRLQDVANDRSSSH

Target ORF information:

RefSeq Version	XM_005163190.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163190.3

ORF Insert Sequence:

ATGATCAGCG ATGACCTGGT GAACTCGTAT CATCTGCTGC TCTGTGCTCT GGATCTGGTT 
TATTGTAACG CGCTGCTGTG CTCGAGCCGG AAAGATCTGC TCAACCCAGC CTTTAAAGGG 
TTGCCTGAGG ACTTCAACAG TAAAGATTAT AAGCCGGGCC CGGGTTCGTT ATGTTTCATA 
GAGCAGCTTT GTGAGCTTCA CGATGGTCTG GTGCTGGAGG CCAAAGGAGC TAAAGAGCAT 
TTCTGGAAAC CCTTCATTAA GAAATTGTTT GAGCGAAAGA TTCTGAAAGG AAAGGAGGAC 
ACGCTGACAG GATTTTTGGA GCCAGTGAAT TTCGGTGACA GTCTGGCGTC TCTGAACCGT 
GTGTATGAAG AACATGTCCT GTCATCCGGT TCTCTGGATG AGCGAATATT TCTGGGAGAT 
GGAGCTAGAG AAGACATCGG CACACCAGGA CCTTACGAAG GGATAGAGAA CCAGGACAGA 
GCGAACAACC CACTGCAGAA CTCGATGACG GCGTCTGCCC TCAGAGTGTC CACTCCATTG 
ACAGGCAGGA GGTACGTGCC GGAGAGCATT GTGGGGACTC CTGTTTCCAG CGCCATGCAG 
AGTGTGGGCC GACTGCACAC CCTGCTGACG GGATTCAAAT ACAGGCCCAG CGTCAAACTC 
AGAGATATAT TTAGATCCTG TGTAAGAGAC CCCACAGAGT CAGTAGCAGC CAGACTGAAG 
GAGATGTCCG AGATTTTCCT TCAGAATTAT GAGGGAACCG GAGACGACAA CAAGAGCCTG 
GCGAGAGATA TCGCAGTGAA GTATTTTTGC TTGGCGGAGG CACTTTACTA TAAATCCCTG 
GAGGTGATCA TCAACCAGGA GAAGAAACGT CTTGGAGACG TGGACCTGTC TGGCGTTCTG 
GAGCAGGATG TGTTTCACCG CTCGCTGATC GCCTGCTGTC TGGAGATCGT CATCTTCTCC 
TATAGACCAC CAGGAGAGTT TCCCAGAGTC CTGCAAATCT TTGACCTCCC CGCTTATCAC 
TTTTACAAGG TGATCGAGGT GTTGGTACGA GCTGAGGAGG GTCTTTTCCG GGAGGTGGTC 
AAACATCTGA ACCATGTCGA GGAGCAGGTG CTGGAGTCTT TGGCATGGAA AGGAAACTCC 
CCGCTGTGGG AGCGAATCAG AGAGGCCAAA AACTGTGTTC CCACCTGCCA GGAGGTAATG 
CCACCTCAAC ACCTAGAGGA CGGTTCAGGA AGCAACGCAA ACACCCCAAG CAGCAAGACT 
GAATTCAGCG GCAACATCGC AGCGGGAGCA GGAAAAGGAG TTTCTCCGTC CCCTACATCT 
CTCCACGACC GCTACAGTTC TCCCCCTACT GGCACAGCAG TTCGTCGTCT TTTTGTGGAT 
GGAGACACGA CGCCAGAACC TGCTCCACCT GTCAAAGTGG CACCGCCGTC TATAGTCAGC 
TCCATCCCAG CAGGACAGAC TGTGGTTACG ATGGCAACAG CTACTGTCAC AGCGAACAAC 
GGCCAGACTG TTACCATACC TGTTCAAGGA ATAGCGAATG AGAGTGGAGG AATCACATTT 
ATCCCAGTGC AAGTGAGTGT GACGAGTCAG GCTGGAGCGA CTCTGCCAAC CCTCAGTGCG 
CAGACTCTGA CAGGCACCCT GACCACTCAG CAGCTCACAA CAACACCTAT GGGCGCCGTG 
CAAAACAACC CCAGCCCTCC CAAACAGGAC AAAAAGACAC CTCAGCAGGG AACACCCACC 
AAACGGGCCC AGAAAACAGG CTCTCTCTGC CTGTTCTTCC GCAAGGTGTA TCATCTAGCT 
AGTGTGCGTC TCAGAGACTT GTGTGCGAAA CTGGACATCA GTGCGGATCT GCGCAGGAAG 
ATCTGGACCT GTTTCGAGTA TTCGCTTGTC CACTGCACCG ACCTCATGAT GGACCGACAT 
CTTGACCAGC TCCTCATGTG CGCCATCTAC GTCATGACTA AGGTGACGAA AGAGGACAAG 
TCGTTCCAGA ACATCATGAA GTGCTACAGA TCTCAGCCTC AGGCCAGCAG CAGTGTGTAC 
AGAAGTGTGC TGATCTCTGG GAGAAAGAGA CGACACTCTG GAAACACTGA AAACACCCAC 
AGACAGAGCT CACCCACTGA GGGAGCACAA GAACAAGCAA GCGGTGAGAG CAGTCCCGTG 
TCCATGCGCT CCAGCAGTAC GCTGCCCATT CCCCAGCCTA GCAGCGCTCC ATCCACCCCG 
ACCCGGGCAC CGGGAGGGCC CCAGGAGCAG GAGGAGGAGC GGGGGGACCT CATCCGCTTC 
TACAACCACG TCTACATCAA ACAGATCAAA CCCTTCGCTC TGCGCTACTC CAGCAACTCG 
CCCAAAAATG GGCAGGCTGA AACTCCTCCG CTCTGTCCGT ATCCGTCGCT GCGCATCGGC 
TCTCCACGCC GCGTCCTCCT ATCCCACAAT CACTCCATCT ACATCTCTCC GCACAAGACC 
GGCAGCCCCA TATCTCCACG AGACAAAATC TTCTACTACG TCAGCTCCAG CCCGTCACAC 
CGGCTCCGAG AGATCAACAC CATGATCCGA ACCGGAGAGA CGCCCACCAA GAAGCGCAGC 
ATCGCTCTGG AGGAAGAGCA GCAGTCTCCT GCTAAAAGAC TGTGTCAGGA GAACCAGACG 
GCGCTGCTCA GACGCCTGCA GGACGTGGCT AATGACCGCA GCTCCTCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBL2 ( XM_009298209.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296484.1 Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa29709
Clone ID Related Accession (Same CDS sequence)	XM_005163190.3 , XM_009298209.2 , XM_005163190.4
Accession Version	XM_009298209.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	retinoblastoma-like protein 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGCG ATGACCTGGT GAACTCGTAT CATCTGCTGC TCTGTGCTCT GGATCTGGTT 
TATTGTAACG CGCTGCTGTG CTCGAGCCGG AAAGATCTGC TCAACCCAGC CTTTAAAGGG 
TTGCCTGAGG ACTTCAACAG TAAAGATTAT AAGCCGGGCC CGGGTTCGTT ATGTTTCATA 
GAGCAGCTTT GTGAGCTTCA CGATGGTCTG GTGCTGGAGG CCAAAGGAGC TAAAGAGCAT 
TTCTGGAAAC CCTTCATTAA GAAATTGTTT GAGCGAAAGA TTCTGAAAGG AAAGGAGGAC 
ACGCTGACAG GATTTTTGGA GCCAGTGAAT TTCGGTGACA GTCTGGCGTC TCTGAACCGT 
GTGTATGAAG AACATGTCCT GTCATCCGGT TCTCTGGATG AGCGAATATT TCTGGGAGAT 
GGAGCTAGAG AAGACATCGG CACACCAGGA CCTTACGAAG GGATAGAGAA CCAGGACAGA 
GCGAACAACC CACTGCAGAA CTCGATGACG GCGTCTGCCC TCAGAGTGTC CACTCCATTG 
ACAGGCAGGA GGTACGTGCC GGAGAGCATT GTGGGGACTC CTGTTTCCAG CGCCATGCAG 
AGTGTGGGCC GACTGCACAC CCTGCTGACG GGATTCAAAT ACAGGCCCAG CGTCAAACTC 
AGAGATATAT TTAGATCCTG TGTAAGAGAC CCCACAGAGT CAGTAGCAGC CAGACTGAAG 
GAGATGTCCG AGATTTTCCT TCAGAATTAT GAGGGAACCG GAGACGACAA CAAGAGCCTG 
GCGAGAGATA TCGCAGTGAA GTATTTTTGC TTGGCGGAGG CACTTTACTA TAAATCCCTG 
GAGGTGATCA TCAACCAGGA GAAGAAACGT CTTGGAGACG TGGACCTGTC TGGCGTTCTG 
GAGCAGGATG TGTTTCACCG CTCGCTGATC GCCTGCTGTC TGGAGATCGT CATCTTCTCC 
TATAGACCAC CAGGAGAGTT TCCCAGAGTC CTGCAAATCT TTGACCTCCC CGCTTATCAC 
TTTTACAAGG TGATCGAGGT GTTGGTACGA GCTGAGGAGG GTCTTTTCCG GGAGGTGGTC 
AAACATCTGA ACCATGTCGA GGAGCAGGTG CTGGAGTCTT TGGCATGGAA AGGAAACTCC 
CCGCTGTGGG AGCGAATCAG AGAGGCCAAA AACTGTGTTC CCACCTGCCA GGAGGTAATG 
CCACCTCAAC ACCTAGAGGA CGGTTCAGGA AGCAACGCAA ACACCCCAAG CAGCAAGACT 
GAATTCAGCG GCAACATCGC AGCGGGAGCA GGAAAAGGAG TTTCTCCGTC CCCTACATCT 
CTCCACGACC GCTACAGTTC TCCCCCTACT GGCACAGCAG TTCGTCGTCT TTTTGTGGAT 
GGAGACACGA CGCCAGAACC TGCTCCACCT GTCAAAGTGG CACCGCCGTC TATAGTCAGC 
TCCATCCCAG CAGGACAGAC TGTGGTTACG ATGGCAACAG CTACTGTCAC AGCGAACAAC 
GGCCAGACTG TTACCATACC TGTTCAAGGA ATAGCGAATG AGAGTGGAGG AATCACATTT 
ATCCCAGTGC AAGTGAGTGT GACGAGTCAG GCTGGAGCGA CTCTGCCAAC CCTCAGTGCG 
CAGACTCTGA CAGGCACCCT GACCACTCAG CAGCTCACAA CAACACCTAT GGGCGCCGTG 
CAAAACAACC CCAGCCCTCC CAAACAGGAC AAAAAGACAC CTCAGCAGGG AACACCCACC 
AAACGGGCCC AGAAAACAGG CTCTCTCTGC CTGTTCTTCC GCAAGGTGTA TCATCTAGCT 
AGTGTGCGTC TCAGAGACTT GTGTGCGAAA CTGGACATCA GTGCGGATCT GCGCAGGAAG 
ATCTGGACCT GTTTCGAGTA TTCGCTTGTC CACTGCACCG ACCTCATGAT GGACCGACAT 
CTTGACCAGC TCCTCATGTG CGCCATCTAC GTCATGACTA AGGTGACGAA AGAGGACAAG 
TCGTTCCAGA ACATCATGAA GTGCTACAGA TCTCAGCCTC AGGCCAGCAG CAGTGTGTAC 
AGAAGTGTGC TGATCTCTGG GAGAAAGAGA CGACACTCTG GAAACACTGA AAACACCCAC 
AGACAGAGCT CACCCACTGA GGGAGCACAA GAACAAGCAA GCGGTGAGAG CAGTCCCGTG 
TCCATGCGCT CCAGCAGTAC GCTGCCCATT CCCCAGCCTA GCAGCGCTCC ATCCACCCCG 
ACCCGGGCAC CGGGAGGGCC CCAGGAGCAG GAGGAGGAGC GGGGGGACCT CATCCGCTTC 
TACAACCACG TCTACATCAA ACAGATCAAA CCCTTCGCTC TGCGCTACTC CAGCAACTCG 
CCCAAAAATG GGCAGGCTGA AACTCCTCCG CTCTGTCCGT ATCCGTCGCT GCGCATCGGC 
TCTCCACGCC GCGTCCTCCT ATCCCACAAT CACTCCATCT ACATCTCTCC GCACAAGACC 
GGCAGCCCCA TATCTCCACG AGACAAAATC TTCTACTACG TCAGCTCCAG CCCGTCACAC 
CGGCTCCGAG AGATCAACAC CATGATCCGA ACCGGAGAGA CGCCCACCAA GAAGCGCAGC 
ATCGCTCTGG AGGAAGAGCA GCAGTCTCCT GCTAAAAGAC TGTGTCAGGA GAACCAGACG 
GCGCTGCTCA GACGCCTGCA GGACGTGGCT AATGACCGCA GCTCCTCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296484.1
CDS	93..2786
Translation	MISDDLVNSYHLLLCALDLVYCNALLCSSRKDLLNPAFKGLPEDFNSKDYKPGPGSLCFIEQLCELHDGLVLEAKGAKEHFWKPFIKKLFERKILKGKEDTLTGFLEPVNFGDSLASLNRVYEEHVLSSGSLDERIFLGDGAREDIGTPGPYEGIENQDRANNPLQNSMTASALRVSTPLTGRRYVPESIVGTPVSSAMQSVGRLHTLLTGFKYRPSVKLRDIFRSCVRDPTESVAARLKEMSEIFLQNYEGTGDDNKSLARDIAVKYFCLAEALYYKSLEVIINQEKKRLGDVDLSGVLEQDVFHRSLIACCLEIVIFSYRPPGEFPRVLQIFDLPAYHFYKVIEVLVRAEEGLFREVVKHLNHVEEQVLESLAWKGNSPLWERIREAKNCVPTCQEVMPPQHLEDGSGSNANTPSSKTEFSGNIAAGAGKGVSPSPTSLHDRYSSPPTGTAVRRLFVDGDTTPEPAPPVKVAPPSIVSSIPAGQTVVTMATATVTANNGQTVTIPVQGIANESGGITFIPVQVSVTSQAGATLPTLSAQTLTGTLTTQQLTTTPMGAVQNNPSPPKQDKKTPQQGTPTKRAQKTGSLCLFFRKVYHLASVRLRDLCAKLDISADLRRKIWTCFEYSLVHCTDLMMDRHLDQLLMCAIYVMTKVTKEDKSFQNIMKCYRSQPQASSSVYRSVLISGRKRRHSGNTENTHRQSSPTEGAQEQASGESSPVSMRSSSTLPIPQPSSAPSTPTRAPGGPQEQEEERGDLIRFYNHVYIKQIKPFALRYSSNSPKNGQAETPPLCPYPSLRIGSPRRVLLSHNHSIYISPHKTGSPISPRDKIFYYVSSSPSHRLREINTMIRTGETPTKKRSIALEEEQQSPAKRLCQENQTALLRRLQDVANDRSSSH

Target ORF information:

RefSeq Version	XM_009298209.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298209.2

ORF Insert Sequence:

ATGATCAGCG ATGACCTGGT GAACTCGTAT CATCTGCTGC TCTGTGCTCT GGATCTGGTT 
TATTGTAACG CGCTGCTGTG CTCGAGCCGG AAAGATCTGC TCAACCCAGC CTTTAAAGGG 
TTGCCTGAGG ACTTCAACAG TAAAGATTAT AAGCCGGGCC CGGGTTCGTT ATGTTTCATA 
GAGCAGCTTT GTGAGCTTCA CGATGGTCTG GTGCTGGAGG CCAAAGGAGC TAAAGAGCAT 
TTCTGGAAAC CCTTCATTAA GAAATTGTTT GAGCGAAAGA TTCTGAAAGG AAAGGAGGAC 
ACGCTGACAG GATTTTTGGA GCCAGTGAAT TTCGGTGACA GTCTGGCGTC TCTGAACCGT 
GTGTATGAAG AACATGTCCT GTCATCCGGT TCTCTGGATG AGCGAATATT TCTGGGAGAT 
GGAGCTAGAG AAGACATCGG CACACCAGGA CCTTACGAAG GGATAGAGAA CCAGGACAGA 
GCGAACAACC CACTGCAGAA CTCGATGACG GCGTCTGCCC TCAGAGTGTC CACTCCATTG 
ACAGGCAGGA GGTACGTGCC GGAGAGCATT GTGGGGACTC CTGTTTCCAG CGCCATGCAG 
AGTGTGGGCC GACTGCACAC CCTGCTGACG GGATTCAAAT ACAGGCCCAG CGTCAAACTC 
AGAGATATAT TTAGATCCTG TGTAAGAGAC CCCACAGAGT CAGTAGCAGC CAGACTGAAG 
GAGATGTCCG AGATTTTCCT TCAGAATTAT GAGGGAACCG GAGACGACAA CAAGAGCCTG 
GCGAGAGATA TCGCAGTGAA GTATTTTTGC TTGGCGGAGG CACTTTACTA TAAATCCCTG 
GAGGTGATCA TCAACCAGGA GAAGAAACGT CTTGGAGACG TGGACCTGTC TGGCGTTCTG 
GAGCAGGATG TGTTTCACCG CTCGCTGATC GCCTGCTGTC TGGAGATCGT CATCTTCTCC 
TATAGACCAC CAGGAGAGTT TCCCAGAGTC CTGCAAATCT TTGACCTCCC CGCTTATCAC 
TTTTACAAGG TGATCGAGGT GTTGGTACGA GCTGAGGAGG GTCTTTTCCG GGAGGTGGTC 
AAACATCTGA ACCATGTCGA GGAGCAGGTG CTGGAGTCTT TGGCATGGAA AGGAAACTCC 
CCGCTGTGGG AGCGAATCAG AGAGGCCAAA AACTGTGTTC CCACCTGCCA GGAGGTAATG 
CCACCTCAAC ACCTAGAGGA CGGTTCAGGA AGCAACGCAA ACACCCCAAG CAGCAAGACT 
GAATTCAGCG GCAACATCGC AGCGGGAGCA GGAAAAGGAG TTTCTCCGTC CCCTACATCT 
CTCCACGACC GCTACAGTTC TCCCCCTACT GGCACAGCAG TTCGTCGTCT TTTTGTGGAT 
GGAGACACGA CGCCAGAACC TGCTCCACCT GTCAAAGTGG CACCGCCGTC TATAGTCAGC 
TCCATCCCAG CAGGACAGAC TGTGGTTACG ATGGCAACAG CTACTGTCAC AGCGAACAAC 
GGCCAGACTG TTACCATACC TGTTCAAGGA ATAGCGAATG AGAGTGGAGG AATCACATTT 
ATCCCAGTGC AAGTGAGTGT GACGAGTCAG GCTGGAGCGA CTCTGCCAAC CCTCAGTGCG 
CAGACTCTGA CAGGCACCCT GACCACTCAG CAGCTCACAA CAACACCTAT GGGCGCCGTG 
CAAAACAACC CCAGCCCTCC CAAACAGGAC AAAAAGACAC CTCAGCAGGG AACACCCACC 
AAACGGGCCC AGAAAACAGG CTCTCTCTGC CTGTTCTTCC GCAAGGTGTA TCATCTAGCT 
AGTGTGCGTC TCAGAGACTT GTGTGCGAAA CTGGACATCA GTGCGGATCT GCGCAGGAAG 
ATCTGGACCT GTTTCGAGTA TTCGCTTGTC CACTGCACCG ACCTCATGAT GGACCGACAT 
CTTGACCAGC TCCTCATGTG CGCCATCTAC GTCATGACTA AGGTGACGAA AGAGGACAAG 
TCGTTCCAGA ACATCATGAA GTGCTACAGA TCTCAGCCTC AGGCCAGCAG CAGTGTGTAC 
AGAAGTGTGC TGATCTCTGG GAGAAAGAGA CGACACTCTG GAAACACTGA AAACACCCAC 
AGACAGAGCT CACCCACTGA GGGAGCACAA GAACAAGCAA GCGGTGAGAG CAGTCCCGTG 
TCCATGCGCT CCAGCAGTAC GCTGCCCATT CCCCAGCCTA GCAGCGCTCC ATCCACCCCG 
ACCCGGGCAC CGGGAGGGCC CCAGGAGCAG GAGGAGGAGC GGGGGGACCT CATCCGCTTC 
TACAACCACG TCTACATCAA ACAGATCAAA CCCTTCGCTC TGCGCTACTC CAGCAACTCG 
CCCAAAAATG GGCAGGCTGA AACTCCTCCG CTCTGTCCGT ATCCGTCGCT GCGCATCGGC 
TCTCCACGCC GCGTCCTCCT ATCCCACAAT CACTCCATCT ACATCTCTCC GCACAAGACC 
GGCAGCCCCA TATCTCCACG AGACAAAATC TTCTACTACG TCAGCTCCAG CCCGTCACAC 
CGGCTCCGAG AGATCAACAC CATGATCCGA ACCGGAGAGA CGCCCACCAA GAAGCGCAGC 
ATCGCTCTGG AGGAAGAGCA GCAGTCTCCT GCTAAAAGAC TGTGTCAGGA GAACCAGACG 
GCGCTGCTCA GACGCCTGCA GGACGTGGCT AATGACCGCA GCTCCTCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBL2 ( XM_005163190.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163247.1 Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X3, mRNA.

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CloneID	ODa29709
Clone ID Related Accession (Same CDS sequence)	XM_005163190.3 , XM_009298209.2 , XM_005163190.4
Accession Version	XM_005163190.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	retinoblastoma-like protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163190.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGCG ATGACCTGGT GAACTCGTAT CATCTGCTGC TCTGTGCTCT GGATCTGGTT 
TATTGTAACG CGCTGCTGTG CTCGAGCCGG AAAGATCTGC TCAACCCAGC CTTTAAAGGG 
TTGCCTGAGG ACTTCAACAG TAAAGATTAT AAGCCGGGCC CGGGTTCGTT ATGTTTCATA 
GAGCAGCTTT GTGAGCTTCA CGATGGTCTG GTGCTGGAGG CCAAAGGAGC TAAAGAGCAT 
TTCTGGAAAC CCTTCATTAA GAAATTGTTT GAGCGAAAGA TTCTGAAAGG AAAGGAGGAC 
ACGCTGACAG GATTTTTGGA GCCAGTGAAT TTCGGTGACA GTCTGGCGTC TCTGAACCGT 
GTGTATGAAG AACATGTCCT GTCATCCGGT TCTCTGGATG AGCGAATATT TCTGGGAGAT 
GGAGCTAGAG AAGACATCGG CACACCAGGA CCTTACGAAG GGATAGAGAA CCAGGACAGA 
GCGAACAACC CACTGCAGAA CTCGATGACG GCGTCTGCCC TCAGAGTGTC CACTCCATTG 
ACAGGCAGGA GGTACGTGCC GGAGAGCATT GTGGGGACTC CTGTTTCCAG CGCCATGCAG 
AGTGTGGGCC GACTGCACAC CCTGCTGACG GGATTCAAAT ACAGGCCCAG CGTCAAACTC 
AGAGATATAT TTAGATCCTG TGTAAGAGAC CCCACAGAGT CAGTAGCAGC CAGACTGAAG 
GAGATGTCCG AGATTTTCCT TCAGAATTAT GAGGGAACCG GAGACGACAA CAAGAGCCTG 
GCGAGAGATA TCGCAGTGAA GTATTTTTGC TTGGCGGAGG CACTTTACTA TAAATCCCTG 
GAGGTGATCA TCAACCAGGA GAAGAAACGT CTTGGAGACG TGGACCTGTC TGGCGTTCTG 
GAGCAGGATG TGTTTCACCG CTCGCTGATC GCCTGCTGTC TGGAGATCGT CATCTTCTCC 
TATAGACCAC CAGGAGAGTT TCCCAGAGTC CTGCAAATCT TTGACCTCCC CGCTTATCAC 
TTTTACAAGG TGATCGAGGT GTTGGTACGA GCTGAGGAGG GTCTTTTCCG GGAGGTGGTC 
AAACATCTGA ACCATGTCGA GGAGCAGGTG CTGGAGTCTT TGGCATGGAA AGGAAACTCC 
CCGCTGTGGG AGCGAATCAG AGAGGCCAAA AACTGTGTTC CCACCTGCCA GGAGGTAATG 
CCACCTCAAC ACCTAGAGGA CGGTTCAGGA AGCAACGCAA ACACCCCAAG CAGCAAGACT 
GAATTCAGCG GCAACATCGC AGCGGGAGCA GGAAAAGGAG TTTCTCCGTC CCCTACATCT 
CTCCACGACC GCTACAGTTC TCCCCCTACT GGCACAGCAG TTCGTCGTCT TTTTGTGGAT 
GGAGACACGA CGCCAGAACC TGCTCCACCT GTCAAAGTGG CACCGCCGTC TATAGTCAGC 
TCCATCCCAG CAGGACAGAC TGTGGTTACG ATGGCAACAG CTACTGTCAC AGCGAACAAC 
GGCCAGACTG TTACCATACC TGTTCAAGGA ATAGCGAATG AGAGTGGAGG AATCACATTT 
ATCCCAGTGC AAGTGAGTGT GACGAGTCAG GCTGGAGCGA CTCTGCCAAC CCTCAGTGCG 
CAGACTCTGA CAGGCACCCT GACCACTCAG CAGCTCACAA CAACACCTAT GGGCGCCGTG 
CAAAACAACC CCAGCCCTCC CAAACAGGAC AAAAAGACAC CTCAGCAGGG AACACCCACC 
AAACGGGCCC AGAAAACAGG CTCTCTCTGC CTGTTCTTCC GCAAGGTGTA TCATCTAGCT 
AGTGTGCGTC TCAGAGACTT GTGTGCGAAA CTGGACATCA GTGCGGATCT GCGCAGGAAG 
ATCTGGACCT GTTTCGAGTA TTCGCTTGTC CACTGCACCG ACCTCATGAT GGACCGACAT 
CTTGACCAGC TCCTCATGTG CGCCATCTAC GTCATGACTA AGGTGACGAA AGAGGACAAG 
TCGTTCCAGA ACATCATGAA GTGCTACAGA TCTCAGCCTC AGGCCAGCAG CAGTGTGTAC 
AGAAGTGTGC TGATCTCTGG GAGAAAGAGA CGACACTCTG GAAACACTGA AAACACCCAC 
AGACAGAGCT CACCCACTGA GGGAGCACAA GAACAAGCAA GCGGTGAGAG CAGTCCCGTG 
TCCATGCGCT CCAGCAGTAC GCTGCCCATT CCCCAGCCTA GCAGCGCTCC ATCCACCCCG 
ACCCGGGCAC CGGGAGGGCC CCAGGAGCAG GAGGAGGAGC GGGGGGACCT CATCCGCTTC 
TACAACCACG TCTACATCAA ACAGATCAAA CCCTTCGCTC TGCGCTACTC CAGCAACTCG 
CCCAAAAATG GGCAGGCTGA AACTCCTCCG CTCTGTCCGT ATCCGTCGCT GCGCATCGGC 
TCTCCACGCC GCGTCCTCCT ATCCCACAAT CACTCCATCT ACATCTCTCC GCACAAGACC 
GGCAGCCCCA TATCTCCACG AGACAAAATC TTCTACTACG TCAGCTCCAG CCCGTCACAC 
CGGCTCCGAG AGATCAACAC CATGATCCGA ACCGGAGAGA CGCCCACCAA GAAGCGCAGC 
ATCGCTCTGG AGGAAGAGCA GCAGTCTCCT GCTAAAAGAC TGTGTCAGGA GAACCAGACG 
GCGCTGCTCA GACGCCTGCA GGACGTGGCT AATGACCGCA GCTCCTCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163247.1
CDS	112..2805
Translation	MISDDLVNSYHLLLCALDLVYCNALLCSSRKDLLNPAFKGLPEDFNSKDYKPGPGSLCFIEQLCELHDGLVLEAKGAKEHFWKPFIKKLFERKILKGKEDTLTGFLEPVNFGDSLASLNRVYEEHVLSSGSLDERIFLGDGAREDIGTPGPYEGIENQDRANNPLQNSMTASALRVSTPLTGRRYVPESIVGTPVSSAMQSVGRLHTLLTGFKYRPSVKLRDIFRSCVRDPTESVAARLKEMSEIFLQNYEGTGDDNKSLARDIAVKYFCLAEALYYKSLEVIINQEKKRLGDVDLSGVLEQDVFHRSLIACCLEIVIFSYRPPGEFPRVLQIFDLPAYHFYKVIEVLVRAEEGLFREVVKHLNHVEEQVLESLAWKGNSPLWERIREAKNCVPTCQEVMPPQHLEDGSGSNANTPSSKTEFSGNIAAGAGKGVSPSPTSLHDRYSSPPTGTAVRRLFVDGDTTPEPAPPVKVAPPSIVSSIPAGQTVVTMATATVTANNGQTVTIPVQGIANESGGITFIPVQVSVTSQAGATLPTLSAQTLTGTLTTQQLTTTPMGAVQNNPSPPKQDKKTPQQGTPTKRAQKTGSLCLFFRKVYHLASVRLRDLCAKLDISADLRRKIWTCFEYSLVHCTDLMMDRHLDQLLMCAIYVMTKVTKEDKSFQNIMKCYRSQPQASSSVYRSVLISGRKRRHSGNTENTHRQSSPTEGAQEQASGESSPVSMRSSSTLPIPQPSSAPSTPTRAPGGPQEQEEERGDLIRFYNHVYIKQIKPFALRYSSNSPKNGQAETPPLCPYPSLRIGSPRRVLLSHNHSIYISPHKTGSPISPRDKIFYYVSSSPSHRLREINTMIRTGETPTKKRSIALEEEQQSPAKRLCQENQTALLRRLQDVANDRSSSH

Target ORF information:

RefSeq Version	XM_005163190.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163190.4

ORF Insert Sequence:

ATGATCAGCG ATGACCTGGT GAACTCGTAT CATCTGCTGC TCTGTGCTCT GGATCTGGTT 
TATTGTAACG CGCTGCTGTG CTCGAGCCGG AAAGATCTGC TCAACCCAGC CTTTAAAGGG 
TTGCCTGAGG ACTTCAACAG TAAAGATTAT AAGCCGGGCC CGGGTTCGTT ATGTTTCATA 
GAGCAGCTTT GTGAGCTTCA CGATGGTCTG GTGCTGGAGG CCAAAGGAGC TAAAGAGCAT 
TTCTGGAAAC CCTTCATTAA GAAATTGTTT GAGCGAAAGA TTCTGAAAGG AAAGGAGGAC 
ACGCTGACAG GATTTTTGGA GCCAGTGAAT TTCGGTGACA GTCTGGCGTC TCTGAACCGT 
GTGTATGAAG AACATGTCCT GTCATCCGGT TCTCTGGATG AGCGAATATT TCTGGGAGAT 
GGAGCTAGAG AAGACATCGG CACACCAGGA CCTTACGAAG GGATAGAGAA CCAGGACAGA 
GCGAACAACC CACTGCAGAA CTCGATGACG GCGTCTGCCC TCAGAGTGTC CACTCCATTG 
ACAGGCAGGA GGTACGTGCC GGAGAGCATT GTGGGGACTC CTGTTTCCAG CGCCATGCAG 
AGTGTGGGCC GACTGCACAC CCTGCTGACG GGATTCAAAT ACAGGCCCAG CGTCAAACTC 
AGAGATATAT TTAGATCCTG TGTAAGAGAC CCCACAGAGT CAGTAGCAGC CAGACTGAAG 
GAGATGTCCG AGATTTTCCT TCAGAATTAT GAGGGAACCG GAGACGACAA CAAGAGCCTG 
GCGAGAGATA TCGCAGTGAA GTATTTTTGC TTGGCGGAGG CACTTTACTA TAAATCCCTG 
GAGGTGATCA TCAACCAGGA GAAGAAACGT CTTGGAGACG TGGACCTGTC TGGCGTTCTG 
GAGCAGGATG TGTTTCACCG CTCGCTGATC GCCTGCTGTC TGGAGATCGT CATCTTCTCC 
TATAGACCAC CAGGAGAGTT TCCCAGAGTC CTGCAAATCT TTGACCTCCC CGCTTATCAC 
TTTTACAAGG TGATCGAGGT GTTGGTACGA GCTGAGGAGG GTCTTTTCCG GGAGGTGGTC 
AAACATCTGA ACCATGTCGA GGAGCAGGTG CTGGAGTCTT TGGCATGGAA AGGAAACTCC 
CCGCTGTGGG AGCGAATCAG AGAGGCCAAA AACTGTGTTC CCACCTGCCA GGAGGTAATG 
CCACCTCAAC ACCTAGAGGA CGGTTCAGGA AGCAACGCAA ACACCCCAAG CAGCAAGACT 
GAATTCAGCG GCAACATCGC AGCGGGAGCA GGAAAAGGAG TTTCTCCGTC CCCTACATCT 
CTCCACGACC GCTACAGTTC TCCCCCTACT GGCACAGCAG TTCGTCGTCT TTTTGTGGAT 
GGAGACACGA CGCCAGAACC TGCTCCACCT GTCAAAGTGG CACCGCCGTC TATAGTCAGC 
TCCATCCCAG CAGGACAGAC TGTGGTTACG ATGGCAACAG CTACTGTCAC AGCGAACAAC 
GGCCAGACTG TTACCATACC TGTTCAAGGA ATAGCGAATG AGAGTGGAGG AATCACATTT 
ATCCCAGTGC AAGTGAGTGT GACGAGTCAG GCTGGAGCGA CTCTGCCAAC CCTCAGTGCG 
CAGACTCTGA CAGGCACCCT GACCACTCAG CAGCTCACAA CAACACCTAT GGGCGCCGTG 
CAAAACAACC CCAGCCCTCC CAAACAGGAC AAAAAGACAC CTCAGCAGGG AACACCCACC 
AAACGGGCCC AGAAAACAGG CTCTCTCTGC CTGTTCTTCC GCAAGGTGTA TCATCTAGCT 
AGTGTGCGTC TCAGAGACTT GTGTGCGAAA CTGGACATCA GTGCGGATCT GCGCAGGAAG 
ATCTGGACCT GTTTCGAGTA TTCGCTTGTC CACTGCACCG ACCTCATGAT GGACCGACAT 
CTTGACCAGC TCCTCATGTG CGCCATCTAC GTCATGACTA AGGTGACGAA AGAGGACAAG 
TCGTTCCAGA ACATCATGAA GTGCTACAGA TCTCAGCCTC AGGCCAGCAG CAGTGTGTAC 
AGAAGTGTGC TGATCTCTGG GAGAAAGAGA CGACACTCTG GAAACACTGA AAACACCCAC 
AGACAGAGCT CACCCACTGA GGGAGCACAA GAACAAGCAA GCGGTGAGAG CAGTCCCGTG 
TCCATGCGCT CCAGCAGTAC GCTGCCCATT CCCCAGCCTA GCAGCGCTCC ATCCACCCCG 
ACCCGGGCAC CGGGAGGGCC CCAGGAGCAG GAGGAGGAGC GGGGGGACCT CATCCGCTTC 
TACAACCACG TCTACATCAA ACAGATCAAA CCCTTCGCTC TGCGCTACTC CAGCAACTCG 
CCCAAAAATG GGCAGGCTGA AACTCCTCCG CTCTGTCCGT ATCCGTCGCT GCGCATCGGC 
TCTCCACGCC GCGTCCTCCT ATCCCACAAT CACTCCATCT ACATCTCTCC GCACAAGACC 
GGCAGCCCCA TATCTCCACG AGACAAAATC TTCTACTACG TCAGCTCCAG CCCGTCACAC 
CGGCTCCGAG AGATCAACAC CATGATCCGA ACCGGAGAGA CGCCCACCAA GAAGCGCAGC 
ATCGCTCTGG AGGAAGAGCA GCAGTCTCCT GCTAAAAGAC TGTGTCAGGA GAACCAGACG 
GCGCTGCTCA GACGCCTGCA GGACGTGGCT AATGACCGCA GCTCCTCACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBL2 ( XM_002666954.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002667000.3 Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa29708
Clone ID Related Accession (Same CDS sequence)	XM_002666954.5 , XM_002666954.6
Accession Version	XM_002666954.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	retinoblastoma-like protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002666954.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGG AGGAAGACAC ATTGGGGAAA ACTAAACATG CATTTGAAGA ACTGTGTCGA 
GATCTGAATA TGGATGAGGA GTCGAGTAAC CGAGCCTGGA GGAGTTATGA AAATATCAGC 
AAGAATTACA CTCTGGAGGG CAGTGAGCTG CACTGGTTGG CGTGTTCTCT GTATGTTGCG 
TGTCGCTCAT CAGTGCCCAC TATGAGTAAA GGCACGGCGG AGGGAAACTA CGTGTCTCTG 
ACCAGGATCC TGCGCTCATC ACAGCAGAGC CTGATTGAGT TCTTCAGTAA GATGAAGAAG 
TGGCAGGACA TGGCGAATCT GCCCAGAGAC TTCCAGCAGA GCACAGAGAA ACTGGAGAGG 
AACTTCACCG TCACCTCCGT CATCTTCAAG AAATACCTGC CCTTATTCAG AGACATCTTC 
AAGACACCCA CGGACGAGTT ACCCAGAACA CACCGGGGCA GAAAACAGAG ACGTCATCCC 
TGCACCGTCA CTGAGGTCTT CAACTTCTGC TGGATCCTCT TCATACACGC TAAAGGAAAC 
TTCCCAATGA TCAGCGATGA CCTGGTGAAC TCGTATCATC TGCTGCTCTG TGCTCTGGAT 
CTGGTTTATT GTAACGCGCT GCTGTGCTCG AGCCGGAAAG ATCTGCTCAA CCCAGCCTTT 
AAAGGGTTGC CTGAGGACTT CAACAGTAAA GATTATAAGC CGGGCCCGGG TTCGTTATGT 
TTCATAGAGC AGCTTTGTGA GCTTCACGAT GGTCTGGTGC TGGAGGCCAA AGGAGCTAAA 
GAGCATTTCT GGAAACCCTT CATTAAGAAA TTGTTTGAGC GAAAGATTCT GAAAGGAAAG 
GAGGACACGC TGACAGGATT TTTGGAGCCA GTGAATTTCG GTGACAGTCT GGCGTCTCTG 
AACCGTGTGT ATGAAGAACA TGTCCTGTCA TCCGGTTCTC TGGATGAGCG AATATTTCTG 
GGAGATGGAG CTAGAGAAGA CATCGGCACA CCAGGACCTT ACGAAGGGAT AGAGAACCAG 
GACAGAGCGA ACAACCCACT GCAGAACTCG ATGACGGCGT CTGCCCTCAG AGTGTCCACT 
CCATTGACAG GCAGGAGGTA CGTGCCGGAG AGCATTGTGG GGACTCCTGT TTCCAGCGCC 
ATGCAGAGTG TGGGCCGACT GCACACCCTG CTGACGGGAT TCAAATACAG GCCCAGCGTC 
AAACTCAGAG ATATATTTAG ATCCTGTGTA AGAGACCCCA CAGAGTCAGT AGCAGCCAGA 
CTGAAGGAGA TGTCCGAGAT TTTCCTTCAG AATTATGAGG GAACCGGAGA CGACAACAAG 
AGCCTGGCGA GAGATATCGC AGTGAAGTAT TTTTGCTTGG CGGAGGCACT TTACTATAAA 
TCCCTGGAGG TGATCATCAA CCAGGAGAAG AAACGTCTTG GAGACGTGGA CCTGTCTGGC 
GTTCTGGAGC AGGATGTGTT TCACCGCTCG CTGATCGCCT GCTGTCTGGA GATCGTCATC 
TTCTCCTATA GACCACCAGG AGAGTTTCCC AGAGTCCTGC AAATCTTTGA CCTCCCCGCT 
TATCACTTTT ACAAGGTGAT CGAGGTGTTG GTACGAGCTG AGGAGGGTCT TTTCCGGGAG 
GTGGTCAAAC ATCTGAACCA TGTCGAGGAG CAGGTGCTGG AGTCTTTGGC ATGGAAAGGA 
AACTCCCCGC TGTGGGAGCG AATCAGAGAG GCCAAAAACT GTGTTCCCAC CTGCCAGGAG 
GTAATGCCAC CTCAACACCT AGAGGACGGT TCAGGAAGCA ACGCAAACAC CCCAAGCAGC 
AAGACTGAAT TCAGCGGCAA CATCGCAGCG GGAGCAGGAA AAGGAGTTTC TCCGTCCCCT 
ACATCTCTCC ACGACCGCTA CAGTTCTCCC CCTACTGGCA CAGCAGTTCG TCGTCTTTTT 
GTGGATGGAG ACACGACGCC AGAACCTGCT CCACCTGTCA AAGTGGCACC GCCGTCTATA 
GTCAGCTCCA TCCCAGCAGG ACAGACTGTG GTTACGATGG CAACAGCTAC TGTCACAGCG 
AACAACGGCC AGACTGTTAC CATACCTGTT CAAGGAATAG CGAATGAGAG TGGAGGAATC 
ACATTTATCC CAGTGCAAGT GAGTGTGACG AGTCAGGCTG GAGCGACTCT GCCAACCCTC 
AGTGCGCAGA CTCTGACAGG CACCCTGACC ACTCAGCAGC TCACAACAAC ACCTATGGGC 
GCCGTGCAAA ACAACCCCAG CCCTCCCAAA CAGGACAAAA AGACACCTCA GCAGGGAACA 
CCCACCAAAC GGGCCCAGAA AACAGGCTCT CTCTGCCTGT TCTTCCGCAA GGTGTATCAT 
CTAGCTAGTG TGCGTCTCAG AGACTTGTGT GCGAAACTGG ACATCAGTGC GGATCTGCGC 
AGGAAGATCT GGACCTGTTT CGAGTATTCG CTTGTCCACT GCACCGACCT CATGATGGAC 
CGACATCTTG ACCAGCTCCT CATGTGCGCC ATCTACGTCA TGACTAAGGT GACGAAAGAG 
GACAAGTCGT TCCAGAACAT CATGAAGTGC TACAGATCTC AGCCTCAGGC CAGCAGCAGT 
GTGTACAGAA GTGTGCTGAT CTCTGGGAGA AAGAGACGAC ACTCTGGAAA CACTGAAAAC 
ACCCACAGAC AGAGCTCACC CACTGAGGGA GCACAAGAAC AAGCAAGCGG TGAGAGCAGT 
CCCGTGTCCA TGCGCTCCAG CAGTACGCTG CCCATTCCCC AGCCTAGCAG CGCTCCATCC 
ACCCCGACCC GGGCACCGGG AGGGCCCCAG GAGCAGGAGG AGGAGCGGGG GGACCTCATC 
CGCTTCTACA ACCACGTCTA CATCAAACAG ATCAAACCCT TCGCTCTGCG CTACTCCAGC 
AACTCGCCCA AAAATGGGCA GGCTGAAACT CCTCCGCTCT GTCCGTATCC GTCGCTGCGC 
ATCGGCTCTC CACGCCGCGT CCTCCTATCC CACAATCACT CCATCTACAT CTCTCCGCAC 
AAGACCGGCA GCCCCATATC TCCACGAGAC AAAATCTTCT ACTACGTCAG CTCCAGCCCG 
TCACACCGGC TCCGAGAGAT CAACACCATG ATCCGAACCG GAGAGACGCC CACCAAGAAG 
CGCAGCATCG CTCTGGAGGA AGAGCAGCAG TCTCCTGCTA AAAGACTGTG TCAGGAGAAC 
CAGACGGCGC TGCTCAGACG CCTGCAGGAC GTGGCTAATG ACCGCAGCTC CTCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002667000.3
CDS	218..3457
Translation	MSEEEDTLGKTKHAFEELCRDLNMDEESSNRAWRSYENISKNYTLEGSELHWLACSLYVACRSSVPTMSKGTAEGNYVSLTRILRSSQQSLIEFFSKMKKWQDMANLPRDFQQSTEKLERNFTVTSVIFKKYLPLFRDIFKTPTDELPRTHRGRKQRRHPCTVTEVFNFCWILFIHAKGNFPMISDDLVNSYHLLLCALDLVYCNALLCSSRKDLLNPAFKGLPEDFNSKDYKPGPGSLCFIEQLCELHDGLVLEAKGAKEHFWKPFIKKLFERKILKGKEDTLTGFLEPVNFGDSLASLNRVYEEHVLSSGSLDERIFLGDGAREDIGTPGPYEGIENQDRANNPLQNSMTASALRVSTPLTGRRYVPESIVGTPVSSAMQSVGRLHTLLTGFKYRPSVKLRDIFRSCVRDPTESVAARLKEMSEIFLQNYEGTGDDNKSLARDIAVKYFCLAEALYYKSLEVIINQEKKRLGDVDLSGVLEQDVFHRSLIACCLEIVIFSYRPPGEFPRVLQIFDLPAYHFYKVIEVLVRAEEGLFREVVKHLNHVEEQVLESLAWKGNSPLWERIREAKNCVPTCQEVMPPQHLEDGSGSNANTPSSKTEFSGNIAAGAGKGVSPSPTSLHDRYSSPPTGTAVRRLFVDGDTTPEPAPPVKVAPPSIVSSIPAGQTVVTMATATVTANNGQTVTIPVQGIANESGGITFIPVQVSVTSQAGATLPTLSAQTLTGTLTTQQLTTTPMGAVQNNPSPPKQDKKTPQQGTPTKRAQKTGSLCLFFRKVYHLASVRLRDLCAKLDISADLRRKIWTCFEYSLVHCTDLMMDRHLDQLLMCAIYVMTKVTKEDKSFQNIMKCYRSQPQASSSVYRSVLISGRKRRHSGNTENTHRQSSPTEGAQEQASGESSPVSMRSSSTLPIPQPSSAPSTPTRAPGGPQEQEEERGDLIRFYNHVYIKQIKPFALRYSSNSPKNGQAETPPLCPYPSLRIGSPRRVLLSHNHSIYISPHKTGSPISPRDKIFYYVSSSPSHRLREINTMIRTGETPTKKRSIALEEEQQSPAKRLCQENQTALLRRLQDVANDRSSSH

Target ORF information:

RefSeq Version	XM_002666954.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002666954.5

ORF Insert Sequence:

ATGAGCGAGG AGGAAGACAC ATTGGGGAAA ACTAAACATG CATTTGAAGA ACTGTGTCGA 
GATCTGAATA TGGATGAGGA GTCGAGTAAC CGAGCCTGGA GGAGTTATGA AAATATCAGC 
AAGAATTACA CTCTGGAGGG CAGTGAGCTG CACTGGTTGG CGTGTTCTCT GTATGTTGCG 
TGTCGCTCAT CAGTGCCCAC TATGAGTAAA GGCACGGCGG AGGGAAACTA CGTGTCTCTG 
ACCAGGATCC TGCGCTCATC ACAGCAGAGC CTGATTGAGT TCTTCAGTAA GATGAAGAAG 
TGGCAGGACA TGGCGAATCT GCCCAGAGAC TTCCAGCAGA GCACAGAGAA ACTGGAGAGG 
AACTTCACCG TCACCTCCGT CATCTTCAAG AAATACCTGC CCTTATTCAG AGACATCTTC 
AAGACACCCA CGGACGAGTT ACCCAGAACA CACCGGGGCA GAAAACAGAG ACGTCATCCC 
TGCACCGTCA CTGAGGTCTT CAACTTCTGC TGGATCCTCT TCATACACGC TAAAGGAAAC 
TTCCCAATGA TCAGCGATGA CCTGGTGAAC TCGTATCATC TGCTGCTCTG TGCTCTGGAT 
CTGGTTTATT GTAACGCGCT GCTGTGCTCG AGCCGGAAAG ATCTGCTCAA CCCAGCCTTT 
AAAGGGTTGC CTGAGGACTT CAACAGTAAA GATTATAAGC CGGGCCCGGG TTCGTTATGT 
TTCATAGAGC AGCTTTGTGA GCTTCACGAT GGTCTGGTGC TGGAGGCCAA AGGAGCTAAA 
GAGCATTTCT GGAAACCCTT CATTAAGAAA TTGTTTGAGC GAAAGATTCT GAAAGGAAAG 
GAGGACACGC TGACAGGATT TTTGGAGCCA GTGAATTTCG GTGACAGTCT GGCGTCTCTG 
AACCGTGTGT ATGAAGAACA TGTCCTGTCA TCCGGTTCTC TGGATGAGCG AATATTTCTG 
GGAGATGGAG CTAGAGAAGA CATCGGCACA CCAGGACCTT ACGAAGGGAT AGAGAACCAG 
GACAGAGCGA ACAACCCACT GCAGAACTCG ATGACGGCGT CTGCCCTCAG AGTGTCCACT 
CCATTGACAG GCAGGAGGTA CGTGCCGGAG AGCATTGTGG GGACTCCTGT TTCCAGCGCC 
ATGCAGAGTG TGGGCCGACT GCACACCCTG CTGACGGGAT TCAAATACAG GCCCAGCGTC 
AAACTCAGAG ATATATTTAG ATCCTGTGTA AGAGACCCCA CAGAGTCAGT AGCAGCCAGA 
CTGAAGGAGA TGTCCGAGAT TTTCCTTCAG AATTATGAGG GAACCGGAGA CGACAACAAG 
AGCCTGGCGA GAGATATCGC AGTGAAGTAT TTTTGCTTGG CGGAGGCACT TTACTATAAA 
TCCCTGGAGG TGATCATCAA CCAGGAGAAG AAACGTCTTG GAGACGTGGA CCTGTCTGGC 
GTTCTGGAGC AGGATGTGTT TCACCGCTCG CTGATCGCCT GCTGTCTGGA GATCGTCATC 
TTCTCCTATA GACCACCAGG AGAGTTTCCC AGAGTCCTGC AAATCTTTGA CCTCCCCGCT 
TATCACTTTT ACAAGGTGAT CGAGGTGTTG GTACGAGCTG AGGAGGGTCT TTTCCGGGAG 
GTGGTCAAAC ATCTGAACCA TGTCGAGGAG CAGGTGCTGG AGTCTTTGGC ATGGAAAGGA 
AACTCCCCGC TGTGGGAGCG AATCAGAGAG GCCAAAAACT GTGTTCCCAC CTGCCAGGAG 
GTAATGCCAC CTCAACACCT AGAGGACGGT TCAGGAAGCA ACGCAAACAC CCCAAGCAGC 
AAGACTGAAT TCAGCGGCAA CATCGCAGCG GGAGCAGGAA AAGGAGTTTC TCCGTCCCCT 
ACATCTCTCC ACGACCGCTA CAGTTCTCCC CCTACTGGCA CAGCAGTTCG TCGTCTTTTT 
GTGGATGGAG ACACGACGCC AGAACCTGCT CCACCTGTCA AAGTGGCACC GCCGTCTATA 
GTCAGCTCCA TCCCAGCAGG ACAGACTGTG GTTACGATGG CAACAGCTAC TGTCACAGCG 
AACAACGGCC AGACTGTTAC CATACCTGTT CAAGGAATAG CGAATGAGAG TGGAGGAATC 
ACATTTATCC CAGTGCAAGT GAGTGTGACG AGTCAGGCTG GAGCGACTCT GCCAACCCTC 
AGTGCGCAGA CTCTGACAGG CACCCTGACC ACTCAGCAGC TCACAACAAC ACCTATGGGC 
GCCGTGCAAA ACAACCCCAG CCCTCCCAAA CAGGACAAAA AGACACCTCA GCAGGGAACA 
CCCACCAAAC GGGCCCAGAA AACAGGCTCT CTCTGCCTGT TCTTCCGCAA GGTGTATCAT 
CTAGCTAGTG TGCGTCTCAG AGACTTGTGT GCGAAACTGG ACATCAGTGC GGATCTGCGC 
AGGAAGATCT GGACCTGTTT CGAGTATTCG CTTGTCCACT GCACCGACCT CATGATGGAC 
CGACATCTTG ACCAGCTCCT CATGTGCGCC ATCTACGTCA TGACTAAGGT GACGAAAGAG 
GACAAGTCGT TCCAGAACAT CATGAAGTGC TACAGATCTC AGCCTCAGGC CAGCAGCAGT 
GTGTACAGAA GTGTGCTGAT CTCTGGGAGA AAGAGACGAC ACTCTGGAAA CACTGAAAAC 
ACCCACAGAC AGAGCTCACC CACTGAGGGA GCACAAGAAC AAGCAAGCGG TGAGAGCAGT 
CCCGTGTCCA TGCGCTCCAG CAGTACGCTG CCCATTCCCC AGCCTAGCAG CGCTCCATCC 
ACCCCGACCC GGGCACCGGG AGGGCCCCAG GAGCAGGAGG AGGAGCGGGG GGACCTCATC 
CGCTTCTACA ACCACGTCTA CATCAAACAG ATCAAACCCT TCGCTCTGCG CTACTCCAGC 
AACTCGCCCA AAAATGGGCA GGCTGAAACT CCTCCGCTCT GTCCGTATCC GTCGCTGCGC 
ATCGGCTCTC CACGCCGCGT CCTCCTATCC CACAATCACT CCATCTACAT CTCTCCGCAC 
AAGACCGGCA GCCCCATATC TCCACGAGAC AAAATCTTCT ACTACGTCAG CTCCAGCCCG 
TCACACCGGC TCCGAGAGAT CAACACCATG ATCCGAACCG GAGAGACGCC CACCAAGAAG 
CGCAGCATCG CTCTGGAGGA AGAGCAGCAG TCTCCTGCTA AAAGACTGTG TCAGGAGAAC 
CAGACGGCGC TGCTCAGACG CCTGCAGGAC GTGGCTAATG ACCGCAGCTC CTCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBL2 ( XM_002666954.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002667000.3 Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa29708
Clone ID Related Accession (Same CDS sequence)	XM_002666954.5 , XM_002666954.6
Accession Version	XM_002666954.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	retinoblastoma-like protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002666954.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGG AGGAAGACAC ATTGGGGAAA ACTAAACATG CATTTGAAGA ACTGTGTCGA 
GATCTGAATA TGGATGAGGA GTCGAGTAAC CGAGCCTGGA GGAGTTATGA AAATATCAGC 
AAGAATTACA CTCTGGAGGG CAGTGAGCTG CACTGGTTGG CGTGTTCTCT GTATGTTGCG 
TGTCGCTCAT CAGTGCCCAC TATGAGTAAA GGCACGGCGG AGGGAAACTA CGTGTCTCTG 
ACCAGGATCC TGCGCTCATC ACAGCAGAGC CTGATTGAGT TCTTCAGTAA GATGAAGAAG 
TGGCAGGACA TGGCGAATCT GCCCAGAGAC TTCCAGCAGA GCACAGAGAA ACTGGAGAGG 
AACTTCACCG TCACCTCCGT CATCTTCAAG AAATACCTGC CCTTATTCAG AGACATCTTC 
AAGACACCCA CGGACGAGTT ACCCAGAACA CACCGGGGCA GAAAACAGAG ACGTCATCCC 
TGCACCGTCA CTGAGGTCTT CAACTTCTGC TGGATCCTCT TCATACACGC TAAAGGAAAC 
TTCCCAATGA TCAGCGATGA CCTGGTGAAC TCGTATCATC TGCTGCTCTG TGCTCTGGAT 
CTGGTTTATT GTAACGCGCT GCTGTGCTCG AGCCGGAAAG ATCTGCTCAA CCCAGCCTTT 
AAAGGGTTGC CTGAGGACTT CAACAGTAAA GATTATAAGC CGGGCCCGGG TTCGTTATGT 
TTCATAGAGC AGCTTTGTGA GCTTCACGAT GGTCTGGTGC TGGAGGCCAA AGGAGCTAAA 
GAGCATTTCT GGAAACCCTT CATTAAGAAA TTGTTTGAGC GAAAGATTCT GAAAGGAAAG 
GAGGACACGC TGACAGGATT TTTGGAGCCA GTGAATTTCG GTGACAGTCT GGCGTCTCTG 
AACCGTGTGT ATGAAGAACA TGTCCTGTCA TCCGGTTCTC TGGATGAGCG AATATTTCTG 
GGAGATGGAG CTAGAGAAGA CATCGGCACA CCAGGACCTT ACGAAGGGAT AGAGAACCAG 
GACAGAGCGA ACAACCCACT GCAGAACTCG ATGACGGCGT CTGCCCTCAG AGTGTCCACT 
CCATTGACAG GCAGGAGGTA CGTGCCGGAG AGCATTGTGG GGACTCCTGT TTCCAGCGCC 
ATGCAGAGTG TGGGCCGACT GCACACCCTG CTGACGGGAT TCAAATACAG GCCCAGCGTC 
AAACTCAGAG ATATATTTAG ATCCTGTGTA AGAGACCCCA CAGAGTCAGT AGCAGCCAGA 
CTGAAGGAGA TGTCCGAGAT TTTCCTTCAG AATTATGAGG GAACCGGAGA CGACAACAAG 
AGCCTGGCGA GAGATATCGC AGTGAAGTAT TTTTGCTTGG CGGAGGCACT TTACTATAAA 
TCCCTGGAGG TGATCATCAA CCAGGAGAAG AAACGTCTTG GAGACGTGGA CCTGTCTGGC 
GTTCTGGAGC AGGATGTGTT TCACCGCTCG CTGATCGCCT GCTGTCTGGA GATCGTCATC 
TTCTCCTATA GACCACCAGG AGAGTTTCCC AGAGTCCTGC AAATCTTTGA CCTCCCCGCT 
TATCACTTTT ACAAGGTGAT CGAGGTGTTG GTACGAGCTG AGGAGGGTCT TTTCCGGGAG 
GTGGTCAAAC ATCTGAACCA TGTCGAGGAG CAGGTGCTGG AGTCTTTGGC ATGGAAAGGA 
AACTCCCCGC TGTGGGAGCG AATCAGAGAG GCCAAAAACT GTGTTCCCAC CTGCCAGGAG 
GTAATGCCAC CTCAACACCT AGAGGACGGT TCAGGAAGCA ACGCAAACAC CCCAAGCAGC 
AAGACTGAAT TCAGCGGCAA CATCGCAGCG GGAGCAGGAA AAGGAGTTTC TCCGTCCCCT 
ACATCTCTCC ACGACCGCTA CAGTTCTCCC CCTACTGGCA CAGCAGTTCG TCGTCTTTTT 
GTGGATGGAG ACACGACGCC AGAACCTGCT CCACCTGTCA AAGTGGCACC GCCGTCTATA 
GTCAGCTCCA TCCCAGCAGG ACAGACTGTG GTTACGATGG CAACAGCTAC TGTCACAGCG 
AACAACGGCC AGACTGTTAC CATACCTGTT CAAGGAATAG CGAATGAGAG TGGAGGAATC 
ACATTTATCC CAGTGCAAGT GAGTGTGACG AGTCAGGCTG GAGCGACTCT GCCAACCCTC 
AGTGCGCAGA CTCTGACAGG CACCCTGACC ACTCAGCAGC TCACAACAAC ACCTATGGGC 
GCCGTGCAAA ACAACCCCAG CCCTCCCAAA CAGGACAAAA AGACACCTCA GCAGGGAACA 
CCCACCAAAC GGGCCCAGAA AACAGGCTCT CTCTGCCTGT TCTTCCGCAA GGTGTATCAT 
CTAGCTAGTG TGCGTCTCAG AGACTTGTGT GCGAAACTGG ACATCAGTGC GGATCTGCGC 
AGGAAGATCT GGACCTGTTT CGAGTATTCG CTTGTCCACT GCACCGACCT CATGATGGAC 
CGACATCTTG ACCAGCTCCT CATGTGCGCC ATCTACGTCA TGACTAAGGT GACGAAAGAG 
GACAAGTCGT TCCAGAACAT CATGAAGTGC TACAGATCTC AGCCTCAGGC CAGCAGCAGT 
GTGTACAGAA GTGTGCTGAT CTCTGGGAGA AAGAGACGAC ACTCTGGAAA CACTGAAAAC 
ACCCACAGAC AGAGCTCACC CACTGAGGGA GCACAAGAAC AAGCAAGCGG TGAGAGCAGT 
CCCGTGTCCA TGCGCTCCAG CAGTACGCTG CCCATTCCCC AGCCTAGCAG CGCTCCATCC 
ACCCCGACCC GGGCACCGGG AGGGCCCCAG GAGCAGGAGG AGGAGCGGGG GGACCTCATC 
CGCTTCTACA ACCACGTCTA CATCAAACAG ATCAAACCCT TCGCTCTGCG CTACTCCAGC 
AACTCGCCCA AAAATGGGCA GGCTGAAACT CCTCCGCTCT GTCCGTATCC GTCGCTGCGC 
ATCGGCTCTC CACGCCGCGT CCTCCTATCC CACAATCACT CCATCTACAT CTCTCCGCAC 
AAGACCGGCA GCCCCATATC TCCACGAGAC AAAATCTTCT ACTACGTCAG CTCCAGCCCG 
TCACACCGGC TCCGAGAGAT CAACACCATG ATCCGAACCG GAGAGACGCC CACCAAGAAG 
CGCAGCATCG CTCTGGAGGA AGAGCAGCAG TCTCCTGCTA AAAGACTGTG TCAGGAGAAC 
CAGACGGCGC TGCTCAGACG CCTGCAGGAC GTGGCTAATG ACCGCAGCTC CTCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002667000.3
CDS	167..3406
Translation	MSEEEDTLGKTKHAFEELCRDLNMDEESSNRAWRSYENISKNYTLEGSELHWLACSLYVACRSSVPTMSKGTAEGNYVSLTRILRSSQQSLIEFFSKMKKWQDMANLPRDFQQSTEKLERNFTVTSVIFKKYLPLFRDIFKTPTDELPRTHRGRKQRRHPCTVTEVFNFCWILFIHAKGNFPMISDDLVNSYHLLLCALDLVYCNALLCSSRKDLLNPAFKGLPEDFNSKDYKPGPGSLCFIEQLCELHDGLVLEAKGAKEHFWKPFIKKLFERKILKGKEDTLTGFLEPVNFGDSLASLNRVYEEHVLSSGSLDERIFLGDGAREDIGTPGPYEGIENQDRANNPLQNSMTASALRVSTPLTGRRYVPESIVGTPVSSAMQSVGRLHTLLTGFKYRPSVKLRDIFRSCVRDPTESVAARLKEMSEIFLQNYEGTGDDNKSLARDIAVKYFCLAEALYYKSLEVIINQEKKRLGDVDLSGVLEQDVFHRSLIACCLEIVIFSYRPPGEFPRVLQIFDLPAYHFYKVIEVLVRAEEGLFREVVKHLNHVEEQVLESLAWKGNSPLWERIREAKNCVPTCQEVMPPQHLEDGSGSNANTPSSKTEFSGNIAAGAGKGVSPSPTSLHDRYSSPPTGTAVRRLFVDGDTTPEPAPPVKVAPPSIVSSIPAGQTVVTMATATVTANNGQTVTIPVQGIANESGGITFIPVQVSVTSQAGATLPTLSAQTLTGTLTTQQLTTTPMGAVQNNPSPPKQDKKTPQQGTPTKRAQKTGSLCLFFRKVYHLASVRLRDLCAKLDISADLRRKIWTCFEYSLVHCTDLMMDRHLDQLLMCAIYVMTKVTKEDKSFQNIMKCYRSQPQASSSVYRSVLISGRKRRHSGNTENTHRQSSPTEGAQEQASGESSPVSMRSSSTLPIPQPSSAPSTPTRAPGGPQEQEEERGDLIRFYNHVYIKQIKPFALRYSSNSPKNGQAETPPLCPYPSLRIGSPRRVLLSHNHSIYISPHKTGSPISPRDKIFYYVSSSPSHRLREINTMIRTGETPTKKRSIALEEEQQSPAKRLCQENQTALLRRLQDVANDRSSSH

Target ORF information:

RefSeq Version	XM_002666954.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002666954.6

ORF Insert Sequence:

ATGAGCGAGG AGGAAGACAC ATTGGGGAAA ACTAAACATG CATTTGAAGA ACTGTGTCGA 
GATCTGAATA TGGATGAGGA GTCGAGTAAC CGAGCCTGGA GGAGTTATGA AAATATCAGC 
AAGAATTACA CTCTGGAGGG CAGTGAGCTG CACTGGTTGG CGTGTTCTCT GTATGTTGCG 
TGTCGCTCAT CAGTGCCCAC TATGAGTAAA GGCACGGCGG AGGGAAACTA CGTGTCTCTG 
ACCAGGATCC TGCGCTCATC ACAGCAGAGC CTGATTGAGT TCTTCAGTAA GATGAAGAAG 
TGGCAGGACA TGGCGAATCT GCCCAGAGAC TTCCAGCAGA GCACAGAGAA ACTGGAGAGG 
AACTTCACCG TCACCTCCGT CATCTTCAAG AAATACCTGC CCTTATTCAG AGACATCTTC 
AAGACACCCA CGGACGAGTT ACCCAGAACA CACCGGGGCA GAAAACAGAG ACGTCATCCC 
TGCACCGTCA CTGAGGTCTT CAACTTCTGC TGGATCCTCT TCATACACGC TAAAGGAAAC 
TTCCCAATGA TCAGCGATGA CCTGGTGAAC TCGTATCATC TGCTGCTCTG TGCTCTGGAT 
CTGGTTTATT GTAACGCGCT GCTGTGCTCG AGCCGGAAAG ATCTGCTCAA CCCAGCCTTT 
AAAGGGTTGC CTGAGGACTT CAACAGTAAA GATTATAAGC CGGGCCCGGG TTCGTTATGT 
TTCATAGAGC AGCTTTGTGA GCTTCACGAT GGTCTGGTGC TGGAGGCCAA AGGAGCTAAA 
GAGCATTTCT GGAAACCCTT CATTAAGAAA TTGTTTGAGC GAAAGATTCT GAAAGGAAAG 
GAGGACACGC TGACAGGATT TTTGGAGCCA GTGAATTTCG GTGACAGTCT GGCGTCTCTG 
AACCGTGTGT ATGAAGAACA TGTCCTGTCA TCCGGTTCTC TGGATGAGCG AATATTTCTG 
GGAGATGGAG CTAGAGAAGA CATCGGCACA CCAGGACCTT ACGAAGGGAT AGAGAACCAG 
GACAGAGCGA ACAACCCACT GCAGAACTCG ATGACGGCGT CTGCCCTCAG AGTGTCCACT 
CCATTGACAG GCAGGAGGTA CGTGCCGGAG AGCATTGTGG GGACTCCTGT TTCCAGCGCC 
ATGCAGAGTG TGGGCCGACT GCACACCCTG CTGACGGGAT TCAAATACAG GCCCAGCGTC 
AAACTCAGAG ATATATTTAG ATCCTGTGTA AGAGACCCCA CAGAGTCAGT AGCAGCCAGA 
CTGAAGGAGA TGTCCGAGAT TTTCCTTCAG AATTATGAGG GAACCGGAGA CGACAACAAG 
AGCCTGGCGA GAGATATCGC AGTGAAGTAT TTTTGCTTGG CGGAGGCACT TTACTATAAA 
TCCCTGGAGG TGATCATCAA CCAGGAGAAG AAACGTCTTG GAGACGTGGA CCTGTCTGGC 
GTTCTGGAGC AGGATGTGTT TCACCGCTCG CTGATCGCCT GCTGTCTGGA GATCGTCATC 
TTCTCCTATA GACCACCAGG AGAGTTTCCC AGAGTCCTGC AAATCTTTGA CCTCCCCGCT 
TATCACTTTT ACAAGGTGAT CGAGGTGTTG GTACGAGCTG AGGAGGGTCT TTTCCGGGAG 
GTGGTCAAAC ATCTGAACCA TGTCGAGGAG CAGGTGCTGG AGTCTTTGGC ATGGAAAGGA 
AACTCCCCGC TGTGGGAGCG AATCAGAGAG GCCAAAAACT GTGTTCCCAC CTGCCAGGAG 
GTAATGCCAC CTCAACACCT AGAGGACGGT TCAGGAAGCA ACGCAAACAC CCCAAGCAGC 
AAGACTGAAT TCAGCGGCAA CATCGCAGCG GGAGCAGGAA AAGGAGTTTC TCCGTCCCCT 
ACATCTCTCC ACGACCGCTA CAGTTCTCCC CCTACTGGCA CAGCAGTTCG TCGTCTTTTT 
GTGGATGGAG ACACGACGCC AGAACCTGCT CCACCTGTCA AAGTGGCACC GCCGTCTATA 
GTCAGCTCCA TCCCAGCAGG ACAGACTGTG GTTACGATGG CAACAGCTAC TGTCACAGCG 
AACAACGGCC AGACTGTTAC CATACCTGTT CAAGGAATAG CGAATGAGAG TGGAGGAATC 
ACATTTATCC CAGTGCAAGT GAGTGTGACG AGTCAGGCTG GAGCGACTCT GCCAACCCTC 
AGTGCGCAGA CTCTGACAGG CACCCTGACC ACTCAGCAGC TCACAACAAC ACCTATGGGC 
GCCGTGCAAA ACAACCCCAG CCCTCCCAAA CAGGACAAAA AGACACCTCA GCAGGGAACA 
CCCACCAAAC GGGCCCAGAA AACAGGCTCT CTCTGCCTGT TCTTCCGCAA GGTGTATCAT 
CTAGCTAGTG TGCGTCTCAG AGACTTGTGT GCGAAACTGG ACATCAGTGC GGATCTGCGC 
AGGAAGATCT GGACCTGTTT CGAGTATTCG CTTGTCCACT GCACCGACCT CATGATGGAC 
CGACATCTTG ACCAGCTCCT CATGTGCGCC ATCTACGTCA TGACTAAGGT GACGAAAGAG 
GACAAGTCGT TCCAGAACAT CATGAAGTGC TACAGATCTC AGCCTCAGGC CAGCAGCAGT 
GTGTACAGAA GTGTGCTGAT CTCTGGGAGA AAGAGACGAC ACTCTGGAAA CACTGAAAAC 
ACCCACAGAC AGAGCTCACC CACTGAGGGA GCACAAGAAC AAGCAAGCGG TGAGAGCAGT 
CCCGTGTCCA TGCGCTCCAG CAGTACGCTG CCCATTCCCC AGCCTAGCAG CGCTCCATCC 
ACCCCGACCC GGGCACCGGG AGGGCCCCAG GAGCAGGAGG AGGAGCGGGG GGACCTCATC 
CGCTTCTACA ACCACGTCTA CATCAAACAG ATCAAACCCT TCGCTCTGCG CTACTCCAGC 
AACTCGCCCA AAAATGGGCA GGCTGAAACT CCTCCGCTCT GTCCGTATCC GTCGCTGCGC 
ATCGGCTCTC CACGCCGCGT CCTCCTATCC CACAATCACT CCATCTACAT CTCTCCGCAC 
AAGACCGGCA GCCCCATATC TCCACGAGAC AAAATCTTCT ACTACGTCAG CTCCAGCCCG 
TCACACCGGC TCCGAGAGAT CAACACCATG ATCCGAACCG GAGAGACGCC CACCAAGAAG 
CGCAGCATCG CTCTGGAGGA AGAGCAGCAG TCTCCTGCTA AAAGACTGTG TCAGGAGAAC 
CAGACGGCGC TGCTCAGACG CCTGCAGGAC GTGGCTAATG ACCGCAGCTC CTCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBL2 ( XM_009298208.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296483.1 Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49546
Clone ID Related Accession (Same CDS sequence)	XM_009298208.3 , XM_009298208.2
Accession Version	XM_009298208.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	retinoblastoma-like protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298208.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGG AGGAAGACAC ATTGGGGAAA ACTAAACATG CATTTGAAGA ACTGTGTCGA 
GATCTGAATA TGGATGAGGA GTCGAGTAAC CGAGCCTGGA GGAGTTATGA AAATATCAGC 
AAGAATTACA CTCTGGAGGG CAGTGAGCTG CACTGGTTGG CGTGTTCTCT GTATGTTGCG 
TGTCGCTCAT CAGTGCCCAC TATGAGTAAA GGCACGGCGG AGGGAAACTA CGTGTCTCTG 
ACCAGGATCC TGCGCTCATC ACAGCAGAGC CTGATTGAGT TCTTCAGTAA GATGAAGAAG 
TGGCAGGACA TGGCGAATCT GCCCAGAGAC TTCCAGCAGA GCACAGAGAA ACTGGAGAGG 
AACTTCACCG TCACCTCCGT CATCTTCAAG AAATACCTGC CCTTATTCAG AGACATCTTC 
AAGACACCCA CGGACGAGTT ACCCAGAACA CACCGGGGCA GAAAACAGAG ACGTCATCCC 
TGCACCGTCA CTGAGGTCTT CAACTTCTGC TGGATCCTCT TCATACACGC TAAAGGAAAC 
TTCCCAATGA TCAGCGATGA CCTGGTGAAC TCGTATCATC TGCTGCTCTG TGCTCTGGAT 
CTGGTTTATT GTAACGCGCT GCTGTGCTCG AGCCGGAAAG ATCTGCTCAA CCCAGCCTTT 
AAAGGGTTGC CTGAGGACTT CAACAGTAAA GATTATAAGC CGGGCCCGGG TTCGTTATGT 
TTCATAGAGC AGCTTTGTGA GCTTCACGAT GGTCTGGTGC TGGAGGCCAA AGGAGCTAAA 
GAGCATTTCT GGAAACCCTT CATTAAGAAA TTGTTTGAGC GAAAGATTCT GAAAGGAAAG 
GAGGACACGC TGACAGGATT TTTGGAGCCA GTGAATTTCG GTGACAGTCT GGCGTCTCTG 
AACCGTGTGT ATGAAGAACA TGTCCTGTCA TCCGGTTCTC TGGATGAGCG AATATTTCTG 
GGAGATGGAG CTAGAGAAGA CATCGGCACA CCAGGACCTT ACGAAGGGAT AGAGAACCAG 
GACAGAGCGA ACAACCCACT GCAGAACTCG ATGACGGCGT CTGCCCTCAG AGTGTCCACT 
CCATTGACAG GCAGGAGGTA CGTGCCGGAG AGCATTGTGG GGACTCCTGT TTCCAGCGCC 
ATGCAGAGTG TGGGCCGACT GCACACCCTG CTGACGGGAT TCAAATACAG GCCCAGCGTC 
AAACTCAGAG ATATATTTAG ATCCTGTGTA AGAGACCCCA CAGAGTCAGT AGCAGCCAGA 
CTGAAGGAGA TGTCCGAGAT TTTCCTTCAG AATTATGAGG GAACCGGAGA CGACAACAAG 
AGCCTGGCGA GAGATATCGC AGTGAAGTAT TTTTGCTTGG CGGAGGCACT TTACTATAAA 
TCCCTGGAGG TGATCATCAA CCAGGAGAAG AAACGTCTTG GAGACGTGGA CCTGTCTGGC 
GTTCTGGAGC AGGATGTGTT TCACCGCTCG CTGATCGCCT GCTGTCTGGA GATCGTCATC 
TTCTCCTATA GACCACCAGG AGAGTTTCCC AGAGTCCTGC AAATCTTTGA CCTCCCCGCT 
TATCACTTTT ACAAGGTGAT CGAGGTGTTG GTACGAGCTG AGGAGGGTCT TTTCCGGGAG 
GTGGTCAAAC ATCTGAACCA TGTCGAGGAG CAGGTGCTGG AGTCTTTGGC ATGGAAAGGA 
AACTCCCCGC TGTGGGAGCG AATCAGAGAG GCCAAAAACT GTGTTCCCAC CTGCCAGGAG 
GTAATGCCAC CTCAACACCT AGAGGACGGT TCAGGAAGCA ACGCAAACAC CCCAAGCAGC 
AAGACTGAAT TCAGCGGCAA CATCGCAGCG GGAGCAGGAA AAGGAGTTTC TCCGTCCCCT 
ACATCTCTCC ACGACCGCTA CAGTTCTCCC CCTACTGGCA CAGCAGTTCG TCGTCTTTTT 
GTGGATGGAG ACACGACGCC AGAACCTGCT CCACCTGTCA AAGTGGCACC GCCGTCTATA 
GTCAGCTCCA TCCCAGCAGG ACAGACTGTG GTTACGATGG CAACAGCTAC TGTCACAGCG 
AACAACGGCC AGACTGTTAC CATACCTGTT CAAGGAATAG CGAATGAGAG TGGAGGAATC 
ACATTTATCC CAGTGCAAGT GAGTGTGACG AGTCAGGCTG GAGCGACTCT GCCAACCCTC 
AGTGCGCAGA CTCTGACAGG CACCCTGACC ACTCAGCAGC TCACAACAAC ACCTATGGGC 
GCCGTGCAAA ACAACCCCAG CCCTCCCAAA CAGGACAAAA AGACACCTCA GCAGGGAACA 
CCCACCAAAC GGGCCCAGAA AACAGGCTCT CTCTGCCTGT TCTTCCGCAA GGTGTATCAT 
CTAGCTAGTG TGCGTCTCAG AGACTTGTGT GCGAAACTGG ACATCAGTGC GGATCTGCGC 
AGGAAGATCT GGACCTGTTT CGAGTATTCG CTTGTCCACT GCACCGACCT CATGATGGAC 
CGACATCTTG ACCAGCTCCT CATGTGCGCC ATCTACGTCA TGACTAAGGT GACGAAAGAG 
GACAAGTCGT TCCAGAACAT CATGAAGTGC TACAGATCTC AGCCTCAGGC CAGCAGCAGT 
GTGTACAGAA GTGTGCTGAT CTCTGGGAGA AAGAGACGAC ACTCTGGAAA CACTGAAAAC 
ACCCACAGAC AGAGCTCACC CACTGAGGGA GCACAAGAAC AAGCAAGCGG TGAGAGCAGT 
CCCGTGTCCA TGCGCTCCAG CAGTACGCTG CCCATTCCCC AGCCTAGCAG CGCTCCATCC 
ACCCCGACCC GGGCACCGGG AGGGCCCCAG GAGCAGGAGG AGGAGCGGGG GGACCTCATC 
CGCTTCTACA ACCACGTCTA CATCAAACAG ATCAAACCCT TCGCTCTGCG CTACTCCAGC 
AACTCGCCCA AAAATGGGGC TGAAACTCCT CCGCTCTGTC CGTATCCGTC GCTGCGCATC 
GGCTCTCCAC GCCGCGTCCT CCTATCCCAC AATCACTCCA TCTACATCTC TCCGCACAAG 
ACCGGCAGCC CCATATCTCC ACGAGACAAA ATCTTCTACT ACGTCAGCTC CAGCCCGTCA 
CACCGGCTCC GAGAGATCAA CACCATGATC CGAACCGGAG AGACGCCCAC CAAGAAGCGC 
AGCATCGCTC TGGAGGAAGA GCAGCAGTCT CCTGCTAAAA GACTGTGTCA GGAGAACCAG 
ACGGCGCTGC TCAGACGCCT GCAGGACGTG GCTAATGACC GCAGCTCCTC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296483.1
CDS	167..3403
Translation	MSEEEDTLGKTKHAFEELCRDLNMDEESSNRAWRSYENISKNYTLEGSELHWLACSLYVACRSSVPTMSKGTAEGNYVSLTRILRSSQQSLIEFFSKMKKWQDMANLPRDFQQSTEKLERNFTVTSVIFKKYLPLFRDIFKTPTDELPRTHRGRKQRRHPCTVTEVFNFCWILFIHAKGNFPMISDDLVNSYHLLLCALDLVYCNALLCSSRKDLLNPAFKGLPEDFNSKDYKPGPGSLCFIEQLCELHDGLVLEAKGAKEHFWKPFIKKLFERKILKGKEDTLTGFLEPVNFGDSLASLNRVYEEHVLSSGSLDERIFLGDGAREDIGTPGPYEGIENQDRANNPLQNSMTASALRVSTPLTGRRYVPESIVGTPVSSAMQSVGRLHTLLTGFKYRPSVKLRDIFRSCVRDPTESVAARLKEMSEIFLQNYEGTGDDNKSLARDIAVKYFCLAEALYYKSLEVIINQEKKRLGDVDLSGVLEQDVFHRSLIACCLEIVIFSYRPPGEFPRVLQIFDLPAYHFYKVIEVLVRAEEGLFREVVKHLNHVEEQVLESLAWKGNSPLWERIREAKNCVPTCQEVMPPQHLEDGSGSNANTPSSKTEFSGNIAAGAGKGVSPSPTSLHDRYSSPPTGTAVRRLFVDGDTTPEPAPPVKVAPPSIVSSIPAGQTVVTMATATVTANNGQTVTIPVQGIANESGGITFIPVQVSVTSQAGATLPTLSAQTLTGTLTTQQLTTTPMGAVQNNPSPPKQDKKTPQQGTPTKRAQKTGSLCLFFRKVYHLASVRLRDLCAKLDISADLRRKIWTCFEYSLVHCTDLMMDRHLDQLLMCAIYVMTKVTKEDKSFQNIMKCYRSQPQASSSVYRSVLISGRKRRHSGNTENTHRQSSPTEGAQEQASGESSPVSMRSSSTLPIPQPSSAPSTPTRAPGGPQEQEEERGDLIRFYNHVYIKQIKPFALRYSSNSPKNGAETPPLCPYPSLRIGSPRRVLLSHNHSIYISPHKTGSPISPRDKIFYYVSSSPSHRLREINTMIRTGETPTKKRSIALEEEQQSPAKRLCQENQTALLRRLQDVANDRSSSH

Target ORF information:

RefSeq Version	XM_009298208.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298208.3

ORF Insert Sequence:

ATGAGCGAGG AGGAAGACAC ATTGGGGAAA ACTAAACATG CATTTGAAGA ACTGTGTCGA 
GATCTGAATA TGGATGAGGA GTCGAGTAAC CGAGCCTGGA GGAGTTATGA AAATATCAGC 
AAGAATTACA CTCTGGAGGG CAGTGAGCTG CACTGGTTGG CGTGTTCTCT GTATGTTGCG 
TGTCGCTCAT CAGTGCCCAC TATGAGTAAA GGCACGGCGG AGGGAAACTA CGTGTCTCTG 
ACCAGGATCC TGCGCTCATC ACAGCAGAGC CTGATTGAGT TCTTCAGTAA GATGAAGAAG 
TGGCAGGACA TGGCGAATCT GCCCAGAGAC TTCCAGCAGA GCACAGAGAA ACTGGAGAGG 
AACTTCACCG TCACCTCCGT CATCTTCAAG AAATACCTGC CCTTATTCAG AGACATCTTC 
AAGACACCCA CGGACGAGTT ACCCAGAACA CACCGGGGCA GAAAACAGAG ACGTCATCCC 
TGCACCGTCA CTGAGGTCTT CAACTTCTGC TGGATCCTCT TCATACACGC TAAAGGAAAC 
TTCCCAATGA TCAGCGATGA CCTGGTGAAC TCGTATCATC TGCTGCTCTG TGCTCTGGAT 
CTGGTTTATT GTAACGCGCT GCTGTGCTCG AGCCGGAAAG ATCTGCTCAA CCCAGCCTTT 
AAAGGGTTGC CTGAGGACTT CAACAGTAAA GATTATAAGC CGGGCCCGGG TTCGTTATGT 
TTCATAGAGC AGCTTTGTGA GCTTCACGAT GGTCTGGTGC TGGAGGCCAA AGGAGCTAAA 
GAGCATTTCT GGAAACCCTT CATTAAGAAA TTGTTTGAGC GAAAGATTCT GAAAGGAAAG 
GAGGACACGC TGACAGGATT TTTGGAGCCA GTGAATTTCG GTGACAGTCT GGCGTCTCTG 
AACCGTGTGT ATGAAGAACA TGTCCTGTCA TCCGGTTCTC TGGATGAGCG AATATTTCTG 
GGAGATGGAG CTAGAGAAGA CATCGGCACA CCAGGACCTT ACGAAGGGAT AGAGAACCAG 
GACAGAGCGA ACAACCCACT GCAGAACTCG ATGACGGCGT CTGCCCTCAG AGTGTCCACT 
CCATTGACAG GCAGGAGGTA CGTGCCGGAG AGCATTGTGG GGACTCCTGT TTCCAGCGCC 
ATGCAGAGTG TGGGCCGACT GCACACCCTG CTGACGGGAT TCAAATACAG GCCCAGCGTC 
AAACTCAGAG ATATATTTAG ATCCTGTGTA AGAGACCCCA CAGAGTCAGT AGCAGCCAGA 
CTGAAGGAGA TGTCCGAGAT TTTCCTTCAG AATTATGAGG GAACCGGAGA CGACAACAAG 
AGCCTGGCGA GAGATATCGC AGTGAAGTAT TTTTGCTTGG CGGAGGCACT TTACTATAAA 
TCCCTGGAGG TGATCATCAA CCAGGAGAAG AAACGTCTTG GAGACGTGGA CCTGTCTGGC 
GTTCTGGAGC AGGATGTGTT TCACCGCTCG CTGATCGCCT GCTGTCTGGA GATCGTCATC 
TTCTCCTATA GACCACCAGG AGAGTTTCCC AGAGTCCTGC AAATCTTTGA CCTCCCCGCT 
TATCACTTTT ACAAGGTGAT CGAGGTGTTG GTACGAGCTG AGGAGGGTCT TTTCCGGGAG 
GTGGTCAAAC ATCTGAACCA TGTCGAGGAG CAGGTGCTGG AGTCTTTGGC ATGGAAAGGA 
AACTCCCCGC TGTGGGAGCG AATCAGAGAG GCCAAAAACT GTGTTCCCAC CTGCCAGGAG 
GTAATGCCAC CTCAACACCT AGAGGACGGT TCAGGAAGCA ACGCAAACAC CCCAAGCAGC 
AAGACTGAAT TCAGCGGCAA CATCGCAGCG GGAGCAGGAA AAGGAGTTTC TCCGTCCCCT 
ACATCTCTCC ACGACCGCTA CAGTTCTCCC CCTACTGGCA CAGCAGTTCG TCGTCTTTTT 
GTGGATGGAG ACACGACGCC AGAACCTGCT CCACCTGTCA AAGTGGCACC GCCGTCTATA 
GTCAGCTCCA TCCCAGCAGG ACAGACTGTG GTTACGATGG CAACAGCTAC TGTCACAGCG 
AACAACGGCC AGACTGTTAC CATACCTGTT CAAGGAATAG CGAATGAGAG TGGAGGAATC 
ACATTTATCC CAGTGCAAGT GAGTGTGACG AGTCAGGCTG GAGCGACTCT GCCAACCCTC 
AGTGCGCAGA CTCTGACAGG CACCCTGACC ACTCAGCAGC TCACAACAAC ACCTATGGGC 
GCCGTGCAAA ACAACCCCAG CCCTCCCAAA CAGGACAAAA AGACACCTCA GCAGGGAACA 
CCCACCAAAC GGGCCCAGAA AACAGGCTCT CTCTGCCTGT TCTTCCGCAA GGTGTATCAT 
CTAGCTAGTG TGCGTCTCAG AGACTTGTGT GCGAAACTGG ACATCAGTGC GGATCTGCGC 
AGGAAGATCT GGACCTGTTT CGAGTATTCG CTTGTCCACT GCACCGACCT CATGATGGAC 
CGACATCTTG ACCAGCTCCT CATGTGCGCC ATCTACGTCA TGACTAAGGT GACGAAAGAG 
GACAAGTCGT TCCAGAACAT CATGAAGTGC TACAGATCTC AGCCTCAGGC CAGCAGCAGT 
GTGTACAGAA GTGTGCTGAT CTCTGGGAGA AAGAGACGAC ACTCTGGAAA CACTGAAAAC 
ACCCACAGAC AGAGCTCACC CACTGAGGGA GCACAAGAAC AAGCAAGCGG TGAGAGCAGT 
CCCGTGTCCA TGCGCTCCAG CAGTACGCTG CCCATTCCCC AGCCTAGCAG CGCTCCATCC 
ACCCCGACCC GGGCACCGGG AGGGCCCCAG GAGCAGGAGG AGGAGCGGGG GGACCTCATC 
CGCTTCTACA ACCACGTCTA CATCAAACAG ATCAAACCCT TCGCTCTGCG CTACTCCAGC 
AACTCGCCCA AAAATGGGGC TGAAACTCCT CCGCTCTGTC CGTATCCGTC GCTGCGCATC 
GGCTCTCCAC GCCGCGTCCT CCTATCCCAC AATCACTCCA TCTACATCTC TCCGCACAAG 
ACCGGCAGCC CCATATCTCC ACGAGACAAA ATCTTCTACT ACGTCAGCTC CAGCCCGTCA 
CACCGGCTCC GAGAGATCAA CACCATGATC CGAACCGGAG AGACGCCCAC CAAGAAGCGC 
AGCATCGCTC TGGAGGAAGA GCAGCAGTCT CCTGCTAAAA GACTGTGTCA GGAGAACCAG 
ACGGCGCTGC TCAGACGCCT GCAGGACGTG GCTAATGACC GCAGCTCCTC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RBL2 ( XM_009298208.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296483.1 Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X2, mRNA.

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CloneID	ODa49546
Clone ID Related Accession (Same CDS sequence)	XM_009298208.3 , XM_009298208.2
Accession Version	XM_009298208.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	retinoblastoma-like protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGG AGGAAGACAC ATTGGGGAAA ACTAAACATG CATTTGAAGA ACTGTGTCGA 
GATCTGAATA TGGATGAGGA GTCGAGTAAC CGAGCCTGGA GGAGTTATGA AAATATCAGC 
AAGAATTACA CTCTGGAGGG CAGTGAGCTG CACTGGTTGG CGTGTTCTCT GTATGTTGCG 
TGTCGCTCAT CAGTGCCCAC TATGAGTAAA GGCACGGCGG AGGGAAACTA CGTGTCTCTG 
ACCAGGATCC TGCGCTCATC ACAGCAGAGC CTGATTGAGT TCTTCAGTAA GATGAAGAAG 
TGGCAGGACA TGGCGAATCT GCCCAGAGAC TTCCAGCAGA GCACAGAGAA ACTGGAGAGG 
AACTTCACCG TCACCTCCGT CATCTTCAAG AAATACCTGC CCTTATTCAG AGACATCTTC 
AAGACACCCA CGGACGAGTT ACCCAGAACA CACCGGGGCA GAAAACAGAG ACGTCATCCC 
TGCACCGTCA CTGAGGTCTT CAACTTCTGC TGGATCCTCT TCATACACGC TAAAGGAAAC 
TTCCCAATGA TCAGCGATGA CCTGGTGAAC TCGTATCATC TGCTGCTCTG TGCTCTGGAT 
CTGGTTTATT GTAACGCGCT GCTGTGCTCG AGCCGGAAAG ATCTGCTCAA CCCAGCCTTT 
AAAGGGTTGC CTGAGGACTT CAACAGTAAA GATTATAAGC CGGGCCCGGG TTCGTTATGT 
TTCATAGAGC AGCTTTGTGA GCTTCACGAT GGTCTGGTGC TGGAGGCCAA AGGAGCTAAA 
GAGCATTTCT GGAAACCCTT CATTAAGAAA TTGTTTGAGC GAAAGATTCT GAAAGGAAAG 
GAGGACACGC TGACAGGATT TTTGGAGCCA GTGAATTTCG GTGACAGTCT GGCGTCTCTG 
AACCGTGTGT ATGAAGAACA TGTCCTGTCA TCCGGTTCTC TGGATGAGCG AATATTTCTG 
GGAGATGGAG CTAGAGAAGA CATCGGCACA CCAGGACCTT ACGAAGGGAT AGAGAACCAG 
GACAGAGCGA ACAACCCACT GCAGAACTCG ATGACGGCGT CTGCCCTCAG AGTGTCCACT 
CCATTGACAG GCAGGAGGTA CGTGCCGGAG AGCATTGTGG GGACTCCTGT TTCCAGCGCC 
ATGCAGAGTG TGGGCCGACT GCACACCCTG CTGACGGGAT TCAAATACAG GCCCAGCGTC 
AAACTCAGAG ATATATTTAG ATCCTGTGTA AGAGACCCCA CAGAGTCAGT AGCAGCCAGA 
CTGAAGGAGA TGTCCGAGAT TTTCCTTCAG AATTATGAGG GAACCGGAGA CGACAACAAG 
AGCCTGGCGA GAGATATCGC AGTGAAGTAT TTTTGCTTGG CGGAGGCACT TTACTATAAA 
TCCCTGGAGG TGATCATCAA CCAGGAGAAG AAACGTCTTG GAGACGTGGA CCTGTCTGGC 
GTTCTGGAGC AGGATGTGTT TCACCGCTCG CTGATCGCCT GCTGTCTGGA GATCGTCATC 
TTCTCCTATA GACCACCAGG AGAGTTTCCC AGAGTCCTGC AAATCTTTGA CCTCCCCGCT 
TATCACTTTT ACAAGGTGAT CGAGGTGTTG GTACGAGCTG AGGAGGGTCT TTTCCGGGAG 
GTGGTCAAAC ATCTGAACCA TGTCGAGGAG CAGGTGCTGG AGTCTTTGGC ATGGAAAGGA 
AACTCCCCGC TGTGGGAGCG AATCAGAGAG GCCAAAAACT GTGTTCCCAC CTGCCAGGAG 
GTAATGCCAC CTCAACACCT AGAGGACGGT TCAGGAAGCA ACGCAAACAC CCCAAGCAGC 
AAGACTGAAT TCAGCGGCAA CATCGCAGCG GGAGCAGGAA AAGGAGTTTC TCCGTCCCCT 
ACATCTCTCC ACGACCGCTA CAGTTCTCCC CCTACTGGCA CAGCAGTTCG TCGTCTTTTT 
GTGGATGGAG ACACGACGCC AGAACCTGCT CCACCTGTCA AAGTGGCACC GCCGTCTATA 
GTCAGCTCCA TCCCAGCAGG ACAGACTGTG GTTACGATGG CAACAGCTAC TGTCACAGCG 
AACAACGGCC AGACTGTTAC CATACCTGTT CAAGGAATAG CGAATGAGAG TGGAGGAATC 
ACATTTATCC CAGTGCAAGT GAGTGTGACG AGTCAGGCTG GAGCGACTCT GCCAACCCTC 
AGTGCGCAGA CTCTGACAGG CACCCTGACC ACTCAGCAGC TCACAACAAC ACCTATGGGC 
GCCGTGCAAA ACAACCCCAG CCCTCCCAAA CAGGACAAAA AGACACCTCA GCAGGGAACA 
CCCACCAAAC GGGCCCAGAA AACAGGCTCT CTCTGCCTGT TCTTCCGCAA GGTGTATCAT 
CTAGCTAGTG TGCGTCTCAG AGACTTGTGT GCGAAACTGG ACATCAGTGC GGATCTGCGC 
AGGAAGATCT GGACCTGTTT CGAGTATTCG CTTGTCCACT GCACCGACCT CATGATGGAC 
CGACATCTTG ACCAGCTCCT CATGTGCGCC ATCTACGTCA TGACTAAGGT GACGAAAGAG 
GACAAGTCGT TCCAGAACAT CATGAAGTGC TACAGATCTC AGCCTCAGGC CAGCAGCAGT 
GTGTACAGAA GTGTGCTGAT CTCTGGGAGA AAGAGACGAC ACTCTGGAAA CACTGAAAAC 
ACCCACAGAC AGAGCTCACC CACTGAGGGA GCACAAGAAC AAGCAAGCGG TGAGAGCAGT 
CCCGTGTCCA TGCGCTCCAG CAGTACGCTG CCCATTCCCC AGCCTAGCAG CGCTCCATCC 
ACCCCGACCC GGGCACCGGG AGGGCCCCAG GAGCAGGAGG AGGAGCGGGG GGACCTCATC 
CGCTTCTACA ACCACGTCTA CATCAAACAG ATCAAACCCT TCGCTCTGCG CTACTCCAGC 
AACTCGCCCA AAAATGGGGC TGAAACTCCT CCGCTCTGTC CGTATCCGTC GCTGCGCATC 
GGCTCTCCAC GCCGCGTCCT CCTATCCCAC AATCACTCCA TCTACATCTC TCCGCACAAG 
ACCGGCAGCC CCATATCTCC ACGAGACAAA ATCTTCTACT ACGTCAGCTC CAGCCCGTCA 
CACCGGCTCC GAGAGATCAA CACCATGATC CGAACCGGAG AGACGCCCAC CAAGAAGCGC 
AGCATCGCTC TGGAGGAAGA GCAGCAGTCT CCTGCTAAAA GACTGTGTCA GGAGAACCAG 
ACGGCGCTGC TCAGACGCCT GCAGGACGTG GCTAATGACC GCAGCTCCTC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296483.1
CDS	219..3455
Translation	MSEEEDTLGKTKHAFEELCRDLNMDEESSNRAWRSYENISKNYTLEGSELHWLACSLYVACRSSVPTMSKGTAEGNYVSLTRILRSSQQSLIEFFSKMKKWQDMANLPRDFQQSTEKLERNFTVTSVIFKKYLPLFRDIFKTPTDELPRTHRGRKQRRHPCTVTEVFNFCWILFIHAKGNFPMISDDLVNSYHLLLCALDLVYCNALLCSSRKDLLNPAFKGLPEDFNSKDYKPGPGSLCFIEQLCELHDGLVLEAKGAKEHFWKPFIKKLFERKILKGKEDTLTGFLEPVNFGDSLASLNRVYEEHVLSSGSLDERIFLGDGAREDIGTPGPYEGIENQDRANNPLQNSMTASALRVSTPLTGRRYVPESIVGTPVSSAMQSVGRLHTLLTGFKYRPSVKLRDIFRSCVRDPTESVAARLKEMSEIFLQNYEGTGDDNKSLARDIAVKYFCLAEALYYKSLEVIINQEKKRLGDVDLSGVLEQDVFHRSLIACCLEIVIFSYRPPGEFPRVLQIFDLPAYHFYKVIEVLVRAEEGLFREVVKHLNHVEEQVLESLAWKGNSPLWERIREAKNCVPTCQEVMPPQHLEDGSGSNANTPSSKTEFSGNIAAGAGKGVSPSPTSLHDRYSSPPTGTAVRRLFVDGDTTPEPAPPVKVAPPSIVSSIPAGQTVVTMATATVTANNGQTVTIPVQGIANESGGITFIPVQVSVTSQAGATLPTLSAQTLTGTLTTQQLTTTPMGAVQNNPSPPKQDKKTPQQGTPTKRAQKTGSLCLFFRKVYHLASVRLRDLCAKLDISADLRRKIWTCFEYSLVHCTDLMMDRHLDQLLMCAIYVMTKVTKEDKSFQNIMKCYRSQPQASSSVYRSVLISGRKRRHSGNTENTHRQSSPTEGAQEQASGESSPVSMRSSSTLPIPQPSSAPSTPTRAPGGPQEQEEERGDLIRFYNHVYIKQIKPFALRYSSNSPKNGAETPPLCPYPSLRIGSPRRVLLSHNHSIYISPHKTGSPISPRDKIFYYVSSSPSHRLREINTMIRTGETPTKKRSIALEEEQQSPAKRLCQENQTALLRRLQDVANDRSSSH

Target ORF information:

RefSeq Version	XM_009298208.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio retinoblastoma-like 2 (p130) (rbl2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298208.2

ORF Insert Sequence:

ATGAGCGAGG AGGAAGACAC ATTGGGGAAA ACTAAACATG CATTTGAAGA ACTGTGTCGA 
GATCTGAATA TGGATGAGGA GTCGAGTAAC CGAGCCTGGA GGAGTTATGA AAATATCAGC 
AAGAATTACA CTCTGGAGGG CAGTGAGCTG CACTGGTTGG CGTGTTCTCT GTATGTTGCG 
TGTCGCTCAT CAGTGCCCAC TATGAGTAAA GGCACGGCGG AGGGAAACTA CGTGTCTCTG 
ACCAGGATCC TGCGCTCATC ACAGCAGAGC CTGATTGAGT TCTTCAGTAA GATGAAGAAG 
TGGCAGGACA TGGCGAATCT GCCCAGAGAC TTCCAGCAGA GCACAGAGAA ACTGGAGAGG 
AACTTCACCG TCACCTCCGT CATCTTCAAG AAATACCTGC CCTTATTCAG AGACATCTTC 
AAGACACCCA CGGACGAGTT ACCCAGAACA CACCGGGGCA GAAAACAGAG ACGTCATCCC 
TGCACCGTCA CTGAGGTCTT CAACTTCTGC TGGATCCTCT TCATACACGC TAAAGGAAAC 
TTCCCAATGA TCAGCGATGA CCTGGTGAAC TCGTATCATC TGCTGCTCTG TGCTCTGGAT 
CTGGTTTATT GTAACGCGCT GCTGTGCTCG AGCCGGAAAG ATCTGCTCAA CCCAGCCTTT 
AAAGGGTTGC CTGAGGACTT CAACAGTAAA GATTATAAGC CGGGCCCGGG TTCGTTATGT 
TTCATAGAGC AGCTTTGTGA GCTTCACGAT GGTCTGGTGC TGGAGGCCAA AGGAGCTAAA 
GAGCATTTCT GGAAACCCTT CATTAAGAAA TTGTTTGAGC GAAAGATTCT GAAAGGAAAG 
GAGGACACGC TGACAGGATT TTTGGAGCCA GTGAATTTCG GTGACAGTCT GGCGTCTCTG 
AACCGTGTGT ATGAAGAACA TGTCCTGTCA TCCGGTTCTC TGGATGAGCG AATATTTCTG 
GGAGATGGAG CTAGAGAAGA CATCGGCACA CCAGGACCTT ACGAAGGGAT AGAGAACCAG 
GACAGAGCGA ACAACCCACT GCAGAACTCG ATGACGGCGT CTGCCCTCAG AGTGTCCACT 
CCATTGACAG GCAGGAGGTA CGTGCCGGAG AGCATTGTGG GGACTCCTGT TTCCAGCGCC 
ATGCAGAGTG TGGGCCGACT GCACACCCTG CTGACGGGAT TCAAATACAG GCCCAGCGTC 
AAACTCAGAG ATATATTTAG ATCCTGTGTA AGAGACCCCA CAGAGTCAGT AGCAGCCAGA 
CTGAAGGAGA TGTCCGAGAT TTTCCTTCAG AATTATGAGG GAACCGGAGA CGACAACAAG 
AGCCTGGCGA GAGATATCGC AGTGAAGTAT TTTTGCTTGG CGGAGGCACT TTACTATAAA 
TCCCTGGAGG TGATCATCAA CCAGGAGAAG AAACGTCTTG GAGACGTGGA CCTGTCTGGC 
GTTCTGGAGC AGGATGTGTT TCACCGCTCG CTGATCGCCT GCTGTCTGGA GATCGTCATC 
TTCTCCTATA GACCACCAGG AGAGTTTCCC AGAGTCCTGC AAATCTTTGA CCTCCCCGCT 
TATCACTTTT ACAAGGTGAT CGAGGTGTTG GTACGAGCTG AGGAGGGTCT TTTCCGGGAG 
GTGGTCAAAC ATCTGAACCA TGTCGAGGAG CAGGTGCTGG AGTCTTTGGC ATGGAAAGGA 
AACTCCCCGC TGTGGGAGCG AATCAGAGAG GCCAAAAACT GTGTTCCCAC CTGCCAGGAG 
GTAATGCCAC CTCAACACCT AGAGGACGGT TCAGGAAGCA ACGCAAACAC CCCAAGCAGC 
AAGACTGAAT TCAGCGGCAA CATCGCAGCG GGAGCAGGAA AAGGAGTTTC TCCGTCCCCT 
ACATCTCTCC ACGACCGCTA CAGTTCTCCC CCTACTGGCA CAGCAGTTCG TCGTCTTTTT 
GTGGATGGAG ACACGACGCC AGAACCTGCT CCACCTGTCA AAGTGGCACC GCCGTCTATA 
GTCAGCTCCA TCCCAGCAGG ACAGACTGTG GTTACGATGG CAACAGCTAC TGTCACAGCG 
AACAACGGCC AGACTGTTAC CATACCTGTT CAAGGAATAG CGAATGAGAG TGGAGGAATC 
ACATTTATCC CAGTGCAAGT GAGTGTGACG AGTCAGGCTG GAGCGACTCT GCCAACCCTC 
AGTGCGCAGA CTCTGACAGG CACCCTGACC ACTCAGCAGC TCACAACAAC ACCTATGGGC 
GCCGTGCAAA ACAACCCCAG CCCTCCCAAA CAGGACAAAA AGACACCTCA GCAGGGAACA 
CCCACCAAAC GGGCCCAGAA AACAGGCTCT CTCTGCCTGT TCTTCCGCAA GGTGTATCAT 
CTAGCTAGTG TGCGTCTCAG AGACTTGTGT GCGAAACTGG ACATCAGTGC GGATCTGCGC 
AGGAAGATCT GGACCTGTTT CGAGTATTCG CTTGTCCACT GCACCGACCT CATGATGGAC 
CGACATCTTG ACCAGCTCCT CATGTGCGCC ATCTACGTCA TGACTAAGGT GACGAAAGAG 
GACAAGTCGT TCCAGAACAT CATGAAGTGC TACAGATCTC AGCCTCAGGC CAGCAGCAGT 
GTGTACAGAA GTGTGCTGAT CTCTGGGAGA AAGAGACGAC ACTCTGGAAA CACTGAAAAC 
ACCCACAGAC AGAGCTCACC CACTGAGGGA GCACAAGAAC AAGCAAGCGG TGAGAGCAGT 
CCCGTGTCCA TGCGCTCCAG CAGTACGCTG CCCATTCCCC AGCCTAGCAG CGCTCCATCC 
ACCCCGACCC GGGCACCGGG AGGGCCCCAG GAGCAGGAGG AGGAGCGGGG GGACCTCATC 
CGCTTCTACA ACCACGTCTA CATCAAACAG ATCAAACCCT TCGCTCTGCG CTACTCCAGC 
AACTCGCCCA AAAATGGGGC TGAAACTCCT CCGCTCTGTC CGTATCCGTC GCTGCGCATC 
GGCTCTCCAC GCCGCGTCCT CCTATCCCAC AATCACTCCA TCTACATCTC TCCGCACAAG 
ACCGGCAGCC CCATATCTCC ACGAGACAAA ATCTTCTACT ACGTCAGCTC CAGCCCGTCA 
CACCGGCTCC GAGAGATCAA CACCATGATC CGAACCGGAG AGACGCCCAC CAAGAAGCGC 
AGCATCGCTC TGGAGGAAGA GCAGCAGTCT CCTGCTAAAA GACTGTGTCA GGAGAACCAG 
ACGGCGCTGC TCAGACGCCT GCAGGACGTG GCTAATGACC GCAGCTCCTC ACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RBL2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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