Polr2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr2a
Entrez Gene ID	363633
Full Name	RNA polymerase II subunit A
Synonyms	Rpo2-1
General protein information	Preferred Names RNA polymerase II subunit A Names DNA-directed RNA polymerase II subunit RPB1 Polymerase (RNA II (DNA directed), large polypeptide polymerase (RNA) II (DNA directed) polypeptide A, 220kDa polymerase (RNA) II subunit A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24

mRNA and Protein(s)

mRNA	Protein	Name
XM_343922.7	XP_343923.3	DNA-directed RNA polymerase II subunit RPB1
XM_001079162.5	XP_001079162.1	DNA-directed RNA polymerase II subunit RPB1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rpb1	NP_595673.1
Pan troglodytes (chimpanzee)	POLR2A	XP_511300.3
Homo sapiens (human)	POLR2A	NP_000928.1
Neurospora crassa	NCU02103	XP_964097.1
Oryza sativa (rice)	Os05g0151000	NP_001054670.2
Canis lupus familiaris (dog)	POLR2A	XP_857844.2
Bos taurus (cattle)	POLR2A	NP_001193242.1
Rattus norvegicus (Norway rat)	Polr2a	XP_343923.3
Caenorhabditis elegans (roundworm)	ama-1	NP_500523.4
Xenopus tropicalis (tropical clawed frog)	polr2a	XP_002943327.2
Macaca mulatta (Rhesus monkey)	POLR2A	XP_001118060.2
Mus musculus (house mouse)	Polr2a	NP_033115.1
Saccharomyces cerevisiae (baker's yeast)	RPO21	NP_010141.1
Arabidopsis thaliana (thale cress)	NRPB1	NP_195305.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007810	XP_317690.3
Magnaporthe oryzae (rice blast fungus)	MGG_04652	XP_003713642.1
Kluyveromyces lactis	KLLA0F05071g	XP_455310.1
Danio rerio (zebrafish)	polr2a	XP_005156282.1
Drosophila melanogaster (fruit fly)	RpII215	NP_511124.1
Eremothecium gossypii	AGOS_ADR086C	NP_984182.2

Gene Ontology

The following Polr2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa18746	XM_343922.7 Latest version!	Rattus norvegicus polymerase (RNA) II subunit A (Polr2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa18746	XM_001079162.5 Latest version!	Rattus norvegicus polymerase (RNA) II subunit A (Polr2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr2a ( XM_343922.7 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_343923.3 Rattus norvegicus polymerase (RNA) II subunit A (Polr2a), mRNA.

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CloneID	ORa18746
Clone ID Related Accession (Same CDS sequence)	XM_343922.7 , XM_001079162.5
Accession Version	XM_343922.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DNA-directed RNA polymerase II subunit RPB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_343922.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGGG GTGGCCCTCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA 
GTGCAGTTCG GCGTCCTGAG TCCGGATGAG TTGAAGCGGA TGTCTGTGAC AGAGGGTGGT 
ATCAAATACC CAGAGACAAC AGAGGGTGGT CGCCCCAAAC TTGGGGGACT AATGGATCCA 
CGACAGGGGG TGATTGAGCG GACTGGCCGT TGTCAAACAT GTGCAGGAAA CATGACCGAG 
TGTCCTGGCC ACTTTGGCCA CATTGAACTG GCCAAGCCTG TGTTTCATGT GGGCTTCCTG 
GTGAAGACAA TGAAGGTTTT GCGCTGTGTC TGCTTCTTTT GCTCCAAACT TCTTGTAGAT 
TCTAACAACC CGAAGATTAA GGACATCCTG GCCAAATCTA AGGGGCAGCC CAAGAAACGG 
CTTACACATG TCTATGACCT TTGCAAGGGC AAAAACATCT GTGAAGGTGG AGAGGAGATG 
GACAACAAGT TTGGTGTGGA GCAGCCTGAG GGGGATGAGG ATCTGACCAA AGAAAAGGGC 
CATGGTGGCT GTGGGCGTTA TCAGCCCCGG ATCCGGCGCT CTGGCCTGGA GTTGTATGCT 
GAATGGAAAC ATGTTAATGA GGACTCTCAG GAGAAGAAGA TCTTGCTGAG TCCAGAACGA 
GTGCATGAAA TCTTCAAACG CATCTCAGAT GAGGAGTGTT TTGTGCTGGG CATGGAGCCT 
CGCTATGCCC GGCCTGAGTG GATGATTGTC ACGGTGTTGC CTGTGCCTCC TCTCTCTGTG 
CGACCTGCTG TGGTGATGCA AGGTTCTGCT CGAAACCAGG ATGATCTGAC TCACAAACTG 
GCTGACATTG TGAAGATCAA CAATCAGCTG CGGCGGAACG AACAGAATGG TGCAGCTGCC 
CATGTCATCG CAGAAGATGT GAAACTTCTC CAGTTCCATG TGGCCACCAT GGTGGACAAC 
GAACTGCCCG GCTTACCCCG TGCCATGCAG AAGTCTGGCC GTCCCCTCAA GTCCCTGAAG 
CAACGGTTGA AGGGCAAGGA AGGACGGGTT CGAGGGAATC TAATGGGCAA GCGAGTGGAC 
TTCTCAGCCC GCACTGTCAT CACCCCTGAC CCTAACCTAT CCATTGACCA AGTTGGTGTG 
CCCCGCTCCA TTGCTGCCAA CATGACCTTT GCAGAGATTG TCACACCTTT CAACATTGAC 
AGACTTCAGG AATTAGTACG CAGGGGAAAC AGCCAGTATC CAGGAGCCAA GTACATCATC 
CGAGACAACG GTGATCGAAT TGACTTACGG TTCCATCCTA AGCCCAGTGA CCTTCACCTA 
CAGACTGGCT ATAAGGTGGA ACGGCACATG TGTGATGGGG ACATTGTTAT CTTCAATCGT 
CAGCCAACTT TGCACAAAAT GTCCATGATG GGGCATCGGG TCCGCATCCT CCCCTGGTCT 
ACATTTCGCT TGAATCTTAG TGTGACAACT CCATACAATG CAGACTTTGA TGGAGATGAG 
ATGAATTTGC ACCTGCCTCA GTCTCTGGAG ACACGGGCAG AGATCCAAGA GTTGGCCATG 
GTACCTCGAA TGATTGTCAC TCCCCAGAGC AATCGACCTG TCATGGGTAT TGTGCAAGAC 
ACACTCACAG CGGTCCGCAA ATTCACTAAG AGAGATGTCT TCCTGGAGCG GGGTGAAGTG 
ATGAACCTCT TAATGTTCCT GTCCACGTGG GATGGGAAGG TCCCACAGCC AGCCATCCTC 
AAGCCCCGGC CCCTGTGGAC AGGCAAGCAA ATCTTCTCCC TCATCATACC TGGTCACATC 
AATTGTATCC GTACTCACAG CACCCATCCT GATGATGAAG ATAGTGGCCC TTACAAGCAC 
ATCTCTCCTG GGGACACCAA AGTGGTGGTG GAGAATGGGG AGCTTATCAT GGGCATCCTT 
TGTAAGAAGT CTCTGGGCAC ATCAGCAGGC TCTCTGGTCC ACATCTCCTA CTTAGAGATG 
GGTCATGACG TTACCCGCCT CTTCTACTCC AACATTCAGA CTGTCATTAA CAACTGGCTT 
CTCATCGAGG GTCATACCAT TGGCATTGGG GACTCCATTG CAGATTCCAA GACTTACCAG 
GACATTCAGA ACACTATTAA GAAAGCTAAA CAGGATGTAA TAGAGGTCAT TGAGAAGGCT 
CATAACAATG AGCTAGAACC CACCCCAGGA AACACATTGC GCCAGACATT TGAGAATCAA 
GTGAATCGCA TTCTCAATGA TGCTCGAGAC AAAACTGGCT CCTCTGCACA GAAATCCCTC 
TCTGAATATA ACAACTTCAA GTCTATGGTG GTGTCTGGAG CTAAAGGGTC CAAGATCAAC 
ATCTCCCAGG TCATTGCCGT TGTCGGGCAG CAGAACGTGG AGGGCAAGCG GATCCCATTT 
GGATTTAAGC ATCGGACTCT TCCTCACTTT ATCAAGGATG ATTATGGTCC CGAGAGCCGA 
GGCTTTGTAG AAAACTCCTA CTTGGCTGGC CTCACTCCCA CTGAGTTCTT CTTCCATGCC 
ATGGGAGGAC GAGAAGGTCT CATTGACACA GCTGTTAAGA CTGCTGAGAC TGGGTATATT 
CAGCGAAGGC TGATAAAATC CATGGAGTCG GTGATGGTGA AATATGATGC AACTGTGAGG 
AACTCCATCA ACCAGGTGGT ACAGCTACGC TATGGAGAGG ACGGCCTAGC AGGCGAGAGC 
GTTGAGTTCC AGAATCTGGC TACACTTAAG CCTTCTAATA AAGCTTTTGA GAAGAAGTTC 
CGTTTTGATT ATACCAATGA GAGGGCCCTG CGGCGGACTC TTCAGGAGGA TCTGGTAAAG 
GATGTGCTGA GCAATGCCCA CATACAGAAT GAGCTAGAAC GGGAATTTGA ACGGATGCGG 
GAAGATCGGG AAGTGCTCAG GGTCATCTTC CCAACTGGTG ACAGCAAGGT GGTCCTCCCT 
TGTAACCTTC TGCGGATGAT CTGGAATGCA CAGAAAATCT TCCATATCAA CCCTCGCCTT 
CCCTCTGACC TGCACCCCAT CAAAGTGGTA GAGGGAGTCA AAGAATTGAG CAAGAAGTTG 
GTAATTGTGA ATGGAGATGA CCCACTAAGT CGGCAGGCAC AGGAGAATGC CACTTTGCTC 
TTCAACATCC ACCTAAGATC CACACTTTGC TCTCGCCGAA TGGCTGAGGA GTTTCGGCTC 
AGTGGAGAGG CCTTTGACTG GCTGCTCGGG GAGATAGAGT CCAAGTTCAA CCAAGCCATT 
GCCCATCCTG GAGAAATGGT GGGAGCTTTG GCTGCTCAGT CTCTTGGAGA ACCTGCCACT 
CAGATGACCC TGAACACTTT CCACTATGCT GGTGTATCTG CCAAGAATGT GACGCTGGGT 
GTACCTCGGC TTAAGGAGCT CATCAACATC TCTAAGAAGC CAAAGACCCC TTCACTCACT 
GTCTTCCTGT TAGGCCAATC TGCCCGAGAC GCTGAGCGGG CCAAGGACAT TCTTTGCCGC 
TTGGAACATA CAACATTGAG GAAGGTGACT GCCAACACAG CCATCTACTA TGACCCCAAC 
CCTCAGAGCA CAGTGGTGGC AGAAGATCAG GAGTGGGTGA ATGTGTACTA TGAGATGCCT 
GACTTTGATG TAGCCCGAAT ATCCCCCTGG CTGCTGCGTG TGGAACTGGA CCGAAAGCAC 
ATGACTGACC GGAAGCTTAC CATGGAACAG ATTGCTGAAA AAATCAATGC AGGCTTTGGC 
GATGATTTGA ATTGTATCTT CAATGATGAT AATGCAGAGA AGCTGGTCCT TCGTATCCGC 
ATCATGAACA GTGATGAAAA CAAGATGCAG GAGGAGGAAG AGGTGGTGGA TAAGATGGAT 
GATGATGTTT TCCTGCGGTG CATTGAGTCC AACATGCTGA CAGATATGAC CCTGCAGGGT 
ATTGAGCAGA TCAGCAAGGT ATACATGCAC TTACCTCAGA CAGACAACAA GAAGAAGATC 
ATCATCACAG AAGATGGGGA GTTCAAGGCC CTGCAAGAAT GGATCTTGGA GACAGATGGT 
GTGAGCCTGA TGCGGGTGCT GAGCGAGAAG GATGTAGACC CTGTGCGCAC TACGTCCAAT 
GACATCGTGG AGATCTTCAC GGTACTGGGC ATTGAGGCTG TGCGGAAGGC CCTGGAGCGA 
GAACTGTACC ATGTCATCTC CTTTGATGGT TCCTACGTCA ATTATCGGCA TTTGGCTCTT 
CTGTGTGACA CAATGACATG CCGTGGCCAC TTGATGGCCA TCACTCGTCA TGGAGTCAAC 
CGCCAGGACA CTGGACCACT CATGAAATGC TCCTTTGAGG AAACGGTGGA TGTCCTTATG 
GAAGCAGCTG CACATGGGGA GAGTGACCCC ATGAAGGGAG TCTCTGAGAA TATTATGCTG 
GGCCAGCTCG CTCCAGCTGG TACTGGCTGT TTTGACCTCC TGCTTGATGC TGAGAAATGC 
AAATACGGCA TGGAAATCCC CACCAATATC CCCGGACTGG GGGCCGCTGG ACCTACTGGC 
ATGTTCTTTG GCTCTGCACC CAGTCCGATG GGAGGAATCT CTCCTGCAAT GACACCCTGG 
AACCAGGGTG CAACTCCAGC CTATGGTGCC TGGTCCCCAA GTGTTGGGAG TGGGATGACC 
CCAGGAGCAG CTGGCTTCTC TCCCAGTGCT GCATCTGATG CCAGTGGCTT CAGCCCAGGT 
TACTCGCCTG CATGGTCTCC CACACCAGGC TCTCCAGGCT CCCCTGGACC CTCAAGCCCA 
TATATCCCCT CACCAGGTGG TGCTATGTCT CCCAGCTACT CACCAACATC ACCAGCATAT 
GAGCCGCGCT CCCCTGGGGG CTATACACCG CAGAGCCCCT CATACTCCCC TACTTCTCCT 
TCCTACTCCC CAACGTCTCC GTCTTATTCT CCAACCAGTC CCAACTATAG TCCTACCTCA 
CCTAGCTACT CCCCAACCTC TCCTAGCTAT TCCCCAACCT CTCCATCCTA CTCACCAACC 
TCTCCATCCT ACTCACCAAC CTCTCCCAGC TACTCCCCAA CCTCTCCCAG CTACTCCCCA 
ACATCACCCA GCTATTCTCC AACTTCTCCC AGCTACTCAC CAACATCTCC TAGCTATTCC 
CCAACATCTC CCAGCTACTC ACCAACCTCT CCAAGCTATT CTCCCACCTC CCCCAGTTAC 
TCACCGACAT CTCCAAGCTA CTCACCAACT TCTCCAAGTT ACTCACCAAC TTCCCCAAGT 
TACTCACCCA CTAGCCCTAA CTATTCCCCA ACTAGTCCCA ACTATACCCC AACCTCACCC 
AGCTACAGCC CAACCTCACC CAGCTACTCA CCTACTAGTC CAAACTATAC ACCTACCAGC 
CCTAACTACA GCCCAACCTC TCCAAGCTAT TCCCCAACCT CACCCAGTTA CTCTCCCACC 
TCACCCAGCT ACTCTCCCTC GAGCCCACGG TATACACCTC AGTCTCCAAC CTACACACCG 
AGTTCACCAA GCTACAGCCC TAGCTCGCCA AGCTACAGCC CTACTTCCCC CAAGTATACC 
CCAACTAGTC CTTCCTACAG TCCTAGCTCA CCAGAGTATA CCCCAACTTC TCCCAAATAC 
TCACCTACAA GCCCCAAATA TTCACCCACT TCTCCCAAGT ATTCTCCTAC CAGCCCCACT 
TACTCACCCA CCACCCCAAA ATACTCGCCA ACCTCTCCTA CATATTCACC AACCTCTCCA 
GTCTACACCC CGACCTCTCC CAAGTACTCC CCTACTAGTC CTACCTACTC CCCAACTTCT 
CCCAAGTACT CGCCCACCAG TCCCACCTAC TCACCCACCT CTCCCAAGGG CTCCACCTAC 
TCTCCCACTT CTCCTGGCTA CTCCCCCACC AGCCCCACCT ACAGCCTCAC CAGTCCAGCC 
ATCAGCCCAG AAGACAGCGA TGAGGAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_343923.3
CDS	1..5913
Translation	MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDVTRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPTSPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPEDSDEEN

Target ORF information:

RefSeq Version	XM_343922.7
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polymerase (RNA) II subunit A (Polr2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_343922.7

ORF Insert Sequence:

ATGCACGGGG GTGGCCCTCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA 
GTGCAGTTCG GCGTCCTGAG TCCGGATGAG TTGAAGCGGA TGTCTGTGAC AGAGGGTGGT 
ATCAAATACC CAGAGACAAC AGAGGGTGGT CGCCCCAAAC TTGGGGGACT AATGGATCCA 
CGACAGGGGG TGATTGAGCG GACTGGCCGT TGTCAAACAT GTGCAGGAAA CATGACCGAG 
TGTCCTGGCC ACTTTGGCCA CATTGAACTG GCCAAGCCTG TGTTTCATGT GGGCTTCCTG 
GTGAAGACAA TGAAGGTTTT GCGCTGTGTC TGCTTCTTTT GCTCCAAACT TCTTGTAGAT 
TCTAACAACC CGAAGATTAA GGACATCCTG GCCAAATCTA AGGGGCAGCC CAAGAAACGG 
CTTACACATG TCTATGACCT TTGCAAGGGC AAAAACATCT GTGAAGGTGG AGAGGAGATG 
GACAACAAGT TTGGTGTGGA GCAGCCTGAG GGGGATGAGG ATCTGACCAA AGAAAAGGGC 
CATGGTGGCT GTGGGCGTTA TCAGCCCCGG ATCCGGCGCT CTGGCCTGGA GTTGTATGCT 
GAATGGAAAC ATGTTAATGA GGACTCTCAG GAGAAGAAGA TCTTGCTGAG TCCAGAACGA 
GTGCATGAAA TCTTCAAACG CATCTCAGAT GAGGAGTGTT TTGTGCTGGG CATGGAGCCT 
CGCTATGCCC GGCCTGAGTG GATGATTGTC ACGGTGTTGC CTGTGCCTCC TCTCTCTGTG 
CGACCTGCTG TGGTGATGCA AGGTTCTGCT CGAAACCAGG ATGATCTGAC TCACAAACTG 
GCTGACATTG TGAAGATCAA CAATCAGCTG CGGCGGAACG AACAGAATGG TGCAGCTGCC 
CATGTCATCG CAGAAGATGT GAAACTTCTC CAGTTCCATG TGGCCACCAT GGTGGACAAC 
GAACTGCCCG GCTTACCCCG TGCCATGCAG AAGTCTGGCC GTCCCCTCAA GTCCCTGAAG 
CAACGGTTGA AGGGCAAGGA AGGACGGGTT CGAGGGAATC TAATGGGCAA GCGAGTGGAC 
TTCTCAGCCC GCACTGTCAT CACCCCTGAC CCTAACCTAT CCATTGACCA AGTTGGTGTG 
CCCCGCTCCA TTGCTGCCAA CATGACCTTT GCAGAGATTG TCACACCTTT CAACATTGAC 
AGACTTCAGG AATTAGTACG CAGGGGAAAC AGCCAGTATC CAGGAGCCAA GTACATCATC 
CGAGACAACG GTGATCGAAT TGACTTACGG TTCCATCCTA AGCCCAGTGA CCTTCACCTA 
CAGACTGGCT ATAAGGTGGA ACGGCACATG TGTGATGGGG ACATTGTTAT CTTCAATCGT 
CAGCCAACTT TGCACAAAAT GTCCATGATG GGGCATCGGG TCCGCATCCT CCCCTGGTCT 
ACATTTCGCT TGAATCTTAG TGTGACAACT CCATACAATG CAGACTTTGA TGGAGATGAG 
ATGAATTTGC ACCTGCCTCA GTCTCTGGAG ACACGGGCAG AGATCCAAGA GTTGGCCATG 
GTACCTCGAA TGATTGTCAC TCCCCAGAGC AATCGACCTG TCATGGGTAT TGTGCAAGAC 
ACACTCACAG CGGTCCGCAA ATTCACTAAG AGAGATGTCT TCCTGGAGCG GGGTGAAGTG 
ATGAACCTCT TAATGTTCCT GTCCACGTGG GATGGGAAGG TCCCACAGCC AGCCATCCTC 
AAGCCCCGGC CCCTGTGGAC AGGCAAGCAA ATCTTCTCCC TCATCATACC TGGTCACATC 
AATTGTATCC GTACTCACAG CACCCATCCT GATGATGAAG ATAGTGGCCC TTACAAGCAC 
ATCTCTCCTG GGGACACCAA AGTGGTGGTG GAGAATGGGG AGCTTATCAT GGGCATCCTT 
TGTAAGAAGT CTCTGGGCAC ATCAGCAGGC TCTCTGGTCC ACATCTCCTA CTTAGAGATG 
GGTCATGACG TTACCCGCCT CTTCTACTCC AACATTCAGA CTGTCATTAA CAACTGGCTT 
CTCATCGAGG GTCATACCAT TGGCATTGGG GACTCCATTG CAGATTCCAA GACTTACCAG 
GACATTCAGA ACACTATTAA GAAAGCTAAA CAGGATGTAA TAGAGGTCAT TGAGAAGGCT 
CATAACAATG AGCTAGAACC CACCCCAGGA AACACATTGC GCCAGACATT TGAGAATCAA 
GTGAATCGCA TTCTCAATGA TGCTCGAGAC AAAACTGGCT CCTCTGCACA GAAATCCCTC 
TCTGAATATA ACAACTTCAA GTCTATGGTG GTGTCTGGAG CTAAAGGGTC CAAGATCAAC 
ATCTCCCAGG TCATTGCCGT TGTCGGGCAG CAGAACGTGG AGGGCAAGCG GATCCCATTT 
GGATTTAAGC ATCGGACTCT TCCTCACTTT ATCAAGGATG ATTATGGTCC CGAGAGCCGA 
GGCTTTGTAG AAAACTCCTA CTTGGCTGGC CTCACTCCCA CTGAGTTCTT CTTCCATGCC 
ATGGGAGGAC GAGAAGGTCT CATTGACACA GCTGTTAAGA CTGCTGAGAC TGGGTATATT 
CAGCGAAGGC TGATAAAATC CATGGAGTCG GTGATGGTGA AATATGATGC AACTGTGAGG 
AACTCCATCA ACCAGGTGGT ACAGCTACGC TATGGAGAGG ACGGCCTAGC AGGCGAGAGC 
GTTGAGTTCC AGAATCTGGC TACACTTAAG CCTTCTAATA AAGCTTTTGA GAAGAAGTTC 
CGTTTTGATT ATACCAATGA GAGGGCCCTG CGGCGGACTC TTCAGGAGGA TCTGGTAAAG 
GATGTGCTGA GCAATGCCCA CATACAGAAT GAGCTAGAAC GGGAATTTGA ACGGATGCGG 
GAAGATCGGG AAGTGCTCAG GGTCATCTTC CCAACTGGTG ACAGCAAGGT GGTCCTCCCT 
TGTAACCTTC TGCGGATGAT CTGGAATGCA CAGAAAATCT TCCATATCAA CCCTCGCCTT 
CCCTCTGACC TGCACCCCAT CAAAGTGGTA GAGGGAGTCA AAGAATTGAG CAAGAAGTTG 
GTAATTGTGA ATGGAGATGA CCCACTAAGT CGGCAGGCAC AGGAGAATGC CACTTTGCTC 
TTCAACATCC ACCTAAGATC CACACTTTGC TCTCGCCGAA TGGCTGAGGA GTTTCGGCTC 
AGTGGAGAGG CCTTTGACTG GCTGCTCGGG GAGATAGAGT CCAAGTTCAA CCAAGCCATT 
GCCCATCCTG GAGAAATGGT GGGAGCTTTG GCTGCTCAGT CTCTTGGAGA ACCTGCCACT 
CAGATGACCC TGAACACTTT CCACTATGCT GGTGTATCTG CCAAGAATGT GACGCTGGGT 
GTACCTCGGC TTAAGGAGCT CATCAACATC TCTAAGAAGC CAAAGACCCC TTCACTCACT 
GTCTTCCTGT TAGGCCAATC TGCCCGAGAC GCTGAGCGGG CCAAGGACAT TCTTTGCCGC 
TTGGAACATA CAACATTGAG GAAGGTGACT GCCAACACAG CCATCTACTA TGACCCCAAC 
CCTCAGAGCA CAGTGGTGGC AGAAGATCAG GAGTGGGTGA ATGTGTACTA TGAGATGCCT 
GACTTTGATG TAGCCCGAAT ATCCCCCTGG CTGCTGCGTG TGGAACTGGA CCGAAAGCAC 
ATGACTGACC GGAAGCTTAC CATGGAACAG ATTGCTGAAA AAATCAATGC AGGCTTTGGC 
GATGATTTGA ATTGTATCTT CAATGATGAT AATGCAGAGA AGCTGGTCCT TCGTATCCGC 
ATCATGAACA GTGATGAAAA CAAGATGCAG GAGGAGGAAG AGGTGGTGGA TAAGATGGAT 
GATGATGTTT TCCTGCGGTG CATTGAGTCC AACATGCTGA CAGATATGAC CCTGCAGGGT 
ATTGAGCAGA TCAGCAAGGT ATACATGCAC TTACCTCAGA CAGACAACAA GAAGAAGATC 
ATCATCACAG AAGATGGGGA GTTCAAGGCC CTGCAAGAAT GGATCTTGGA GACAGATGGT 
GTGAGCCTGA TGCGGGTGCT GAGCGAGAAG GATGTAGACC CTGTGCGCAC TACGTCCAAT 
GACATCGTGG AGATCTTCAC GGTACTGGGC ATTGAGGCTG TGCGGAAGGC CCTGGAGCGA 
GAACTGTACC ATGTCATCTC CTTTGATGGT TCCTACGTCA ATTATCGGCA TTTGGCTCTT 
CTGTGTGACA CAATGACATG CCGTGGCCAC TTGATGGCCA TCACTCGTCA TGGAGTCAAC 
CGCCAGGACA CTGGACCACT CATGAAATGC TCCTTTGAGG AAACGGTGGA TGTCCTTATG 
GAAGCAGCTG CACATGGGGA GAGTGACCCC ATGAAGGGAG TCTCTGAGAA TATTATGCTG 
GGCCAGCTCG CTCCAGCTGG TACTGGCTGT TTTGACCTCC TGCTTGATGC TGAGAAATGC 
AAATACGGCA TGGAAATCCC CACCAATATC CCCGGACTGG GGGCCGCTGG ACCTACTGGC 
ATGTTCTTTG GCTCTGCACC CAGTCCGATG GGAGGAATCT CTCCTGCAAT GACACCCTGG 
AACCAGGGTG CAACTCCAGC CTATGGTGCC TGGTCCCCAA GTGTTGGGAG TGGGATGACC 
CCAGGAGCAG CTGGCTTCTC TCCCAGTGCT GCATCTGATG CCAGTGGCTT CAGCCCAGGT 
TACTCGCCTG CATGGTCTCC CACACCAGGC TCTCCAGGCT CCCCTGGACC CTCAAGCCCA 
TATATCCCCT CACCAGGTGG TGCTATGTCT CCCAGCTACT CACCAACATC ACCAGCATAT 
GAGCCGCGCT CCCCTGGGGG CTATACACCG CAGAGCCCCT CATACTCCCC TACTTCTCCT 
TCCTACTCCC CAACGTCTCC GTCTTATTCT CCAACCAGTC CCAACTATAG TCCTACCTCA 
CCTAGCTACT CCCCAACCTC TCCTAGCTAT TCCCCAACCT CTCCATCCTA CTCACCAACC 
TCTCCATCCT ACTCACCAAC CTCTCCCAGC TACTCCCCAA CCTCTCCCAG CTACTCCCCA 
ACATCACCCA GCTATTCTCC AACTTCTCCC AGCTACTCAC CAACATCTCC TAGCTATTCC 
CCAACATCTC CCAGCTACTC ACCAACCTCT CCAAGCTATT CTCCCACCTC CCCCAGTTAC 
TCACCGACAT CTCCAAGCTA CTCACCAACT TCTCCAAGTT ACTCACCAAC TTCCCCAAGT 
TACTCACCCA CTAGCCCTAA CTATTCCCCA ACTAGTCCCA ACTATACCCC AACCTCACCC 
AGCTACAGCC CAACCTCACC CAGCTACTCA CCTACTAGTC CAAACTATAC ACCTACCAGC 
CCTAACTACA GCCCAACCTC TCCAAGCTAT TCCCCAACCT CACCCAGTTA CTCTCCCACC 
TCACCCAGCT ACTCTCCCTC GAGCCCACGG TATACACCTC AGTCTCCAAC CTACACACCG 
AGTTCACCAA GCTACAGCCC TAGCTCGCCA AGCTACAGCC CTACTTCCCC CAAGTATACC 
CCAACTAGTC CTTCCTACAG TCCTAGCTCA CCAGAGTATA CCCCAACTTC TCCCAAATAC 
TCACCTACAA GCCCCAAATA TTCACCCACT TCTCCCAAGT ATTCTCCTAC CAGCCCCACT 
TACTCACCCA CCACCCCAAA ATACTCGCCA ACCTCTCCTA CATATTCACC AACCTCTCCA 
GTCTACACCC CGACCTCTCC CAAGTACTCC CCTACTAGTC CTACCTACTC CCCAACTTCT 
CCCAAGTACT CGCCCACCAG TCCCACCTAC TCACCCACCT CTCCCAAGGG CTCCACCTAC 
TCTCCCACTT CTCCTGGCTA CTCCCCCACC AGCCCCACCT ACAGCCTCAC CAGTCCAGCC 
ATCAGCCCAG AAGACAGCGA TGAGGAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Polr2a ( XM_001079162.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001079162.1 Rattus norvegicus polymerase (RNA) II subunit A (Polr2a), mRNA.

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CloneID	ORa18746
Clone ID Related Accession (Same CDS sequence)	XM_343922.7 , XM_001079162.5
Accession Version	XM_001079162.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DNA-directed RNA polymerase II subunit RPB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001079162.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGGG GTGGCCCTCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA 
GTGCAGTTCG GCGTCCTGAG TCCGGATGAG TTGAAGCGGA TGTCTGTGAC AGAGGGTGGT 
ATCAAATACC CAGAGACAAC AGAGGGTGGT CGCCCCAAAC TTGGGGGACT AATGGATCCA 
CGACAGGGGG TGATTGAGCG GACTGGCCGT TGTCAAACAT GTGCAGGAAA CATGACCGAG 
TGTCCTGGCC ACTTTGGCCA CATTGAACTG GCCAAGCCTG TGTTTCATGT GGGCTTCCTG 
GTGAAGACAA TGAAGGTTTT GCGCTGTGTC TGCTTCTTTT GCTCCAAACT TCTTGTAGAT 
TCTAACAACC CGAAGATTAA GGACATCCTG GCCAAATCTA AGGGGCAGCC CAAGAAACGG 
CTTACACATG TCTATGACCT TTGCAAGGGC AAAAACATCT GTGAAGGTGG AGAGGAGATG 
GACAACAAGT TTGGTGTGGA GCAGCCTGAG GGGGATGAGG ATCTGACCAA AGAAAAGGGC 
CATGGTGGCT GTGGGCGTTA TCAGCCCCGG ATCCGGCGCT CTGGCCTGGA GTTGTATGCT 
GAATGGAAAC ATGTTAATGA GGACTCTCAG GAGAAGAAGA TCTTGCTGAG TCCAGAACGA 
GTGCATGAAA TCTTCAAACG CATCTCAGAT GAGGAGTGTT TTGTGCTGGG CATGGAGCCT 
CGCTATGCCC GGCCTGAGTG GATGATTGTC ACGGTGTTGC CTGTGCCTCC TCTCTCTGTG 
CGACCTGCTG TGGTGATGCA AGGTTCTGCT CGAAACCAGG ATGATCTGAC TCACAAACTG 
GCTGACATTG TGAAGATCAA CAATCAGCTG CGGCGGAACG AACAGAATGG TGCAGCTGCC 
CATGTCATCG CAGAAGATGT GAAACTTCTC CAGTTCCATG TGGCCACCAT GGTGGACAAC 
GAACTGCCCG GCTTACCCCG TGCCATGCAG AAGTCTGGCC GTCCCCTCAA GTCCCTGAAG 
CAACGGTTGA AGGGCAAGGA AGGACGGGTT CGAGGGAATC TAATGGGCAA GCGAGTGGAC 
TTCTCAGCCC GCACTGTCAT CACCCCTGAC CCTAACCTAT CCATTGACCA AGTTGGTGTG 
CCCCGCTCCA TTGCTGCCAA CATGACCTTT GCAGAGATTG TCACACCTTT CAACATTGAC 
AGACTTCAGG AATTAGTACG CAGGGGAAAC AGCCAGTATC CAGGAGCCAA GTACATCATC 
CGAGACAACG GTGATCGAAT TGACTTACGG TTCCATCCTA AGCCCAGTGA CCTTCACCTA 
CAGACTGGCT ATAAGGTGGA ACGGCACATG TGTGATGGGG ACATTGTTAT CTTCAATCGT 
CAGCCAACTT TGCACAAAAT GTCCATGATG GGGCATCGGG TCCGCATCCT CCCCTGGTCT 
ACATTTCGCT TGAATCTTAG TGTGACAACT CCATACAATG CAGACTTTGA TGGAGATGAG 
ATGAATTTGC ACCTGCCTCA GTCTCTGGAG ACACGGGCAG AGATCCAAGA GTTGGCCATG 
GTACCTCGAA TGATTGTCAC TCCCCAGAGC AATCGACCTG TCATGGGTAT TGTGCAAGAC 
ACACTCACAG CGGTCCGCAA ATTCACTAAG AGAGATGTCT TCCTGGAGCG GGGTGAAGTG 
ATGAACCTCT TAATGTTCCT GTCCACGTGG GATGGGAAGG TCCCACAGCC AGCCATCCTC 
AAGCCCCGGC CCCTGTGGAC AGGCAAGCAA ATCTTCTCCC TCATCATACC TGGTCACATC 
AATTGTATCC GTACTCACAG CACCCATCCT GATGATGAAG ATAGTGGCCC TTACAAGCAC 
ATCTCTCCTG GGGACACCAA AGTGGTGGTG GAGAATGGGG AGCTTATCAT GGGCATCCTT 
TGTAAGAAGT CTCTGGGCAC ATCAGCAGGC TCTCTGGTCC ACATCTCCTA CTTAGAGATG 
GGTCATGACG TTACCCGCCT CTTCTACTCC AACATTCAGA CTGTCATTAA CAACTGGCTT 
CTCATCGAGG GTCATACCAT TGGCATTGGG GACTCCATTG CAGATTCCAA GACTTACCAG 
GACATTCAGA ACACTATTAA GAAAGCTAAA CAGGATGTAA TAGAGGTCAT TGAGAAGGCT 
CATAACAATG AGCTAGAACC CACCCCAGGA AACACATTGC GCCAGACATT TGAGAATCAA 
GTGAATCGCA TTCTCAATGA TGCTCGAGAC AAAACTGGCT CCTCTGCACA GAAATCCCTC 
TCTGAATATA ACAACTTCAA GTCTATGGTG GTGTCTGGAG CTAAAGGGTC CAAGATCAAC 
ATCTCCCAGG TCATTGCCGT TGTCGGGCAG CAGAACGTGG AGGGCAAGCG GATCCCATTT 
GGATTTAAGC ATCGGACTCT TCCTCACTTT ATCAAGGATG ATTATGGTCC CGAGAGCCGA 
GGCTTTGTAG AAAACTCCTA CTTGGCTGGC CTCACTCCCA CTGAGTTCTT CTTCCATGCC 
ATGGGAGGAC GAGAAGGTCT CATTGACACA GCTGTTAAGA CTGCTGAGAC TGGGTATATT 
CAGCGAAGGC TGATAAAATC CATGGAGTCG GTGATGGTGA AATATGATGC AACTGTGAGG 
AACTCCATCA ACCAGGTGGT ACAGCTACGC TATGGAGAGG ACGGCCTAGC AGGCGAGAGC 
GTTGAGTTCC AGAATCTGGC TACACTTAAG CCTTCTAATA AAGCTTTTGA GAAGAAGTTC 
CGTTTTGATT ATACCAATGA GAGGGCCCTG CGGCGGACTC TTCAGGAGGA TCTGGTAAAG 
GATGTGCTGA GCAATGCCCA CATACAGAAT GAGCTAGAAC GGGAATTTGA ACGGATGCGG 
GAAGATCGGG AAGTGCTCAG GGTCATCTTC CCAACTGGTG ACAGCAAGGT GGTCCTCCCT 
TGTAACCTTC TGCGGATGAT CTGGAATGCA CAGAAAATCT TCCATATCAA CCCTCGCCTT 
CCCTCTGACC TGCACCCCAT CAAAGTGGTA GAGGGAGTCA AAGAATTGAG CAAGAAGTTG 
GTAATTGTGA ATGGAGATGA CCCACTAAGT CGGCAGGCAC AGGAGAATGC CACTTTGCTC 
TTCAACATCC ACCTAAGATC CACACTTTGC TCTCGCCGAA TGGCTGAGGA GTTTCGGCTC 
AGTGGAGAGG CCTTTGACTG GCTGCTCGGG GAGATAGAGT CCAAGTTCAA CCAAGCCATT 
GCCCATCCTG GAGAAATGGT GGGAGCTTTG GCTGCTCAGT CTCTTGGAGA ACCTGCCACT 
CAGATGACCC TGAACACTTT CCACTATGCT GGTGTATCTG CCAAGAATGT GACGCTGGGT 
GTACCTCGGC TTAAGGAGCT CATCAACATC TCTAAGAAGC CAAAGACCCC TTCACTCACT 
GTCTTCCTGT TAGGCCAATC TGCCCGAGAC GCTGAGCGGG CCAAGGACAT TCTTTGCCGC 
TTGGAACATA CAACATTGAG GAAGGTGACT GCCAACACAG CCATCTACTA TGACCCCAAC 
CCTCAGAGCA CAGTGGTGGC AGAAGATCAG GAGTGGGTGA ATGTGTACTA TGAGATGCCT 
GACTTTGATG TAGCCCGAAT ATCCCCCTGG CTGCTGCGTG TGGAACTGGA CCGAAAGCAC 
ATGACTGACC GGAAGCTTAC CATGGAACAG ATTGCTGAAA AAATCAATGC AGGCTTTGGC 
GATGATTTGA ATTGTATCTT CAATGATGAT AATGCAGAGA AGCTGGTCCT TCGTATCCGC 
ATCATGAACA GTGATGAAAA CAAGATGCAG GAGGAGGAAG AGGTGGTGGA TAAGATGGAT 
GATGATGTTT TCCTGCGGTG CATTGAGTCC AACATGCTGA CAGATATGAC CCTGCAGGGT 
ATTGAGCAGA TCAGCAAGGT ATACATGCAC TTACCTCAGA CAGACAACAA GAAGAAGATC 
ATCATCACAG AAGATGGGGA GTTCAAGGCC CTGCAAGAAT GGATCTTGGA GACAGATGGT 
GTGAGCCTGA TGCGGGTGCT GAGCGAGAAG GATGTAGACC CTGTGCGCAC TACGTCCAAT 
GACATCGTGG AGATCTTCAC GGTACTGGGC ATTGAGGCTG TGCGGAAGGC CCTGGAGCGA 
GAACTGTACC ATGTCATCTC CTTTGATGGT TCCTACGTCA ATTATCGGCA TTTGGCTCTT 
CTGTGTGACA CAATGACATG CCGTGGCCAC TTGATGGCCA TCACTCGTCA TGGAGTCAAC 
CGCCAGGACA CTGGACCACT CATGAAATGC TCCTTTGAGG AAACGGTGGA TGTCCTTATG 
GAAGCAGCTG CACATGGGGA GAGTGACCCC ATGAAGGGAG TCTCTGAGAA TATTATGCTG 
GGCCAGCTCG CTCCAGCTGG TACTGGCTGT TTTGACCTCC TGCTTGATGC TGAGAAATGC 
AAATACGGCA TGGAAATCCC CACCAATATC CCCGGACTGG GGGCCGCTGG ACCTACTGGC 
ATGTTCTTTG GCTCTGCACC CAGTCCGATG GGAGGAATCT CTCCTGCAAT GACACCCTGG 
AACCAGGGTG CAACTCCAGC CTATGGTGCC TGGTCCCCAA GTGTTGGGAG TGGGATGACC 
CCAGGAGCAG CTGGCTTCTC TCCCAGTGCT GCATCTGATG CCAGTGGCTT CAGCCCAGGT 
TACTCGCCTG CATGGTCTCC CACACCAGGC TCTCCAGGCT CCCCTGGACC CTCAAGCCCA 
TATATCCCCT CACCAGGTGG TGCTATGTCT CCCAGCTACT CACCAACATC ACCAGCATAT 
GAGCCGCGCT CCCCTGGGGG CTATACACCG CAGAGCCCCT CATACTCCCC TACTTCTCCT 
TCCTACTCCC CAACGTCTCC GTCTTATTCT CCAACCAGTC CCAACTATAG TCCTACCTCA 
CCTAGCTACT CCCCAACCTC TCCTAGCTAT TCCCCAACCT CTCCATCCTA CTCACCAACC 
TCTCCATCCT ACTCACCAAC CTCTCCCAGC TACTCCCCAA CCTCTCCCAG CTACTCCCCA 
ACATCACCCA GCTATTCTCC AACTTCTCCC AGCTACTCAC CAACATCTCC TAGCTATTCC 
CCAACATCTC CCAGCTACTC ACCAACCTCT CCAAGCTATT CTCCCACCTC CCCCAGTTAC 
TCACCGACAT CTCCAAGCTA CTCACCAACT TCTCCAAGTT ACTCACCAAC TTCCCCAAGT 
TACTCACCCA CTAGCCCTAA CTATTCCCCA ACTAGTCCCA ACTATACCCC AACCTCACCC 
AGCTACAGCC CAACCTCACC CAGCTACTCA CCTACTAGTC CAAACTATAC ACCTACCAGC 
CCTAACTACA GCCCAACCTC TCCAAGCTAT TCCCCAACCT CACCCAGTTA CTCTCCCACC 
TCACCCAGCT ACTCTCCCTC GAGCCCACGG TATACACCTC AGTCTCCAAC CTACACACCG 
AGTTCACCAA GCTACAGCCC TAGCTCGCCA AGCTACAGCC CTACTTCCCC CAAGTATACC 
CCAACTAGTC CTTCCTACAG TCCTAGCTCA CCAGAGTATA CCCCAACTTC TCCCAAATAC 
TCACCTACAA GCCCCAAATA TTCACCCACT TCTCCCAAGT ATTCTCCTAC CAGCCCCACT 
TACTCACCCA CCACCCCAAA ATACTCGCCA ACCTCTCCTA CATATTCACC AACCTCTCCA 
GTCTACACCC CGACCTCTCC CAAGTACTCC CCTACTAGTC CTACCTACTC CCCAACTTCT 
CCCAAGTACT CGCCCACCAG TCCCACCTAC TCACCCACCT CTCCCAAGGG CTCCACCTAC 
TCTCCCACTT CTCCTGGCTA CTCCCCCACC AGCCCCACCT ACAGCCTCAC CAGTCCAGCC 
ATCAGCCCAG AAGACAGCGA TGAGGAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001079162.1
CDS	1..5913
Translation	MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDVTRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPTSPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPEDSDEEN

Target ORF information:

RefSeq Version	XM_001079162.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polymerase (RNA) II subunit A (Polr2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001079162.5

ORF Insert Sequence:

ATGCACGGGG GTGGCCCTCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGAGA 
GTGCAGTTCG GCGTCCTGAG TCCGGATGAG TTGAAGCGGA TGTCTGTGAC AGAGGGTGGT 
ATCAAATACC CAGAGACAAC AGAGGGTGGT CGCCCCAAAC TTGGGGGACT AATGGATCCA 
CGACAGGGGG TGATTGAGCG GACTGGCCGT TGTCAAACAT GTGCAGGAAA CATGACCGAG 
TGTCCTGGCC ACTTTGGCCA CATTGAACTG GCCAAGCCTG TGTTTCATGT GGGCTTCCTG 
GTGAAGACAA TGAAGGTTTT GCGCTGTGTC TGCTTCTTTT GCTCCAAACT TCTTGTAGAT 
TCTAACAACC CGAAGATTAA GGACATCCTG GCCAAATCTA AGGGGCAGCC CAAGAAACGG 
CTTACACATG TCTATGACCT TTGCAAGGGC AAAAACATCT GTGAAGGTGG AGAGGAGATG 
GACAACAAGT TTGGTGTGGA GCAGCCTGAG GGGGATGAGG ATCTGACCAA AGAAAAGGGC 
CATGGTGGCT GTGGGCGTTA TCAGCCCCGG ATCCGGCGCT CTGGCCTGGA GTTGTATGCT 
GAATGGAAAC ATGTTAATGA GGACTCTCAG GAGAAGAAGA TCTTGCTGAG TCCAGAACGA 
GTGCATGAAA TCTTCAAACG CATCTCAGAT GAGGAGTGTT TTGTGCTGGG CATGGAGCCT 
CGCTATGCCC GGCCTGAGTG GATGATTGTC ACGGTGTTGC CTGTGCCTCC TCTCTCTGTG 
CGACCTGCTG TGGTGATGCA AGGTTCTGCT CGAAACCAGG ATGATCTGAC TCACAAACTG 
GCTGACATTG TGAAGATCAA CAATCAGCTG CGGCGGAACG AACAGAATGG TGCAGCTGCC 
CATGTCATCG CAGAAGATGT GAAACTTCTC CAGTTCCATG TGGCCACCAT GGTGGACAAC 
GAACTGCCCG GCTTACCCCG TGCCATGCAG AAGTCTGGCC GTCCCCTCAA GTCCCTGAAG 
CAACGGTTGA AGGGCAAGGA AGGACGGGTT CGAGGGAATC TAATGGGCAA GCGAGTGGAC 
TTCTCAGCCC GCACTGTCAT CACCCCTGAC CCTAACCTAT CCATTGACCA AGTTGGTGTG 
CCCCGCTCCA TTGCTGCCAA CATGACCTTT GCAGAGATTG TCACACCTTT CAACATTGAC 
AGACTTCAGG AATTAGTACG CAGGGGAAAC AGCCAGTATC CAGGAGCCAA GTACATCATC 
CGAGACAACG GTGATCGAAT TGACTTACGG TTCCATCCTA AGCCCAGTGA CCTTCACCTA 
CAGACTGGCT ATAAGGTGGA ACGGCACATG TGTGATGGGG ACATTGTTAT CTTCAATCGT 
CAGCCAACTT TGCACAAAAT GTCCATGATG GGGCATCGGG TCCGCATCCT CCCCTGGTCT 
ACATTTCGCT TGAATCTTAG TGTGACAACT CCATACAATG CAGACTTTGA TGGAGATGAG 
ATGAATTTGC ACCTGCCTCA GTCTCTGGAG ACACGGGCAG AGATCCAAGA GTTGGCCATG 
GTACCTCGAA TGATTGTCAC TCCCCAGAGC AATCGACCTG TCATGGGTAT TGTGCAAGAC 
ACACTCACAG CGGTCCGCAA ATTCACTAAG AGAGATGTCT TCCTGGAGCG GGGTGAAGTG 
ATGAACCTCT TAATGTTCCT GTCCACGTGG GATGGGAAGG TCCCACAGCC AGCCATCCTC 
AAGCCCCGGC CCCTGTGGAC AGGCAAGCAA ATCTTCTCCC TCATCATACC TGGTCACATC 
AATTGTATCC GTACTCACAG CACCCATCCT GATGATGAAG ATAGTGGCCC TTACAAGCAC 
ATCTCTCCTG GGGACACCAA AGTGGTGGTG GAGAATGGGG AGCTTATCAT GGGCATCCTT 
TGTAAGAAGT CTCTGGGCAC ATCAGCAGGC TCTCTGGTCC ACATCTCCTA CTTAGAGATG 
GGTCATGACG TTACCCGCCT CTTCTACTCC AACATTCAGA CTGTCATTAA CAACTGGCTT 
CTCATCGAGG GTCATACCAT TGGCATTGGG GACTCCATTG CAGATTCCAA GACTTACCAG 
GACATTCAGA ACACTATTAA GAAAGCTAAA CAGGATGTAA TAGAGGTCAT TGAGAAGGCT 
CATAACAATG AGCTAGAACC CACCCCAGGA AACACATTGC GCCAGACATT TGAGAATCAA 
GTGAATCGCA TTCTCAATGA TGCTCGAGAC AAAACTGGCT CCTCTGCACA GAAATCCCTC 
TCTGAATATA ACAACTTCAA GTCTATGGTG GTGTCTGGAG CTAAAGGGTC CAAGATCAAC 
ATCTCCCAGG TCATTGCCGT TGTCGGGCAG CAGAACGTGG AGGGCAAGCG GATCCCATTT 
GGATTTAAGC ATCGGACTCT TCCTCACTTT ATCAAGGATG ATTATGGTCC CGAGAGCCGA 
GGCTTTGTAG AAAACTCCTA CTTGGCTGGC CTCACTCCCA CTGAGTTCTT CTTCCATGCC 
ATGGGAGGAC GAGAAGGTCT CATTGACACA GCTGTTAAGA CTGCTGAGAC TGGGTATATT 
CAGCGAAGGC TGATAAAATC CATGGAGTCG GTGATGGTGA AATATGATGC AACTGTGAGG 
AACTCCATCA ACCAGGTGGT ACAGCTACGC TATGGAGAGG ACGGCCTAGC AGGCGAGAGC 
GTTGAGTTCC AGAATCTGGC TACACTTAAG CCTTCTAATA AAGCTTTTGA GAAGAAGTTC 
CGTTTTGATT ATACCAATGA GAGGGCCCTG CGGCGGACTC TTCAGGAGGA TCTGGTAAAG 
GATGTGCTGA GCAATGCCCA CATACAGAAT GAGCTAGAAC GGGAATTTGA ACGGATGCGG 
GAAGATCGGG AAGTGCTCAG GGTCATCTTC CCAACTGGTG ACAGCAAGGT GGTCCTCCCT 
TGTAACCTTC TGCGGATGAT CTGGAATGCA CAGAAAATCT TCCATATCAA CCCTCGCCTT 
CCCTCTGACC TGCACCCCAT CAAAGTGGTA GAGGGAGTCA AAGAATTGAG CAAGAAGTTG 
GTAATTGTGA ATGGAGATGA CCCACTAAGT CGGCAGGCAC AGGAGAATGC CACTTTGCTC 
TTCAACATCC ACCTAAGATC CACACTTTGC TCTCGCCGAA TGGCTGAGGA GTTTCGGCTC 
AGTGGAGAGG CCTTTGACTG GCTGCTCGGG GAGATAGAGT CCAAGTTCAA CCAAGCCATT 
GCCCATCCTG GAGAAATGGT GGGAGCTTTG GCTGCTCAGT CTCTTGGAGA ACCTGCCACT 
CAGATGACCC TGAACACTTT CCACTATGCT GGTGTATCTG CCAAGAATGT GACGCTGGGT 
GTACCTCGGC TTAAGGAGCT CATCAACATC TCTAAGAAGC CAAAGACCCC TTCACTCACT 
GTCTTCCTGT TAGGCCAATC TGCCCGAGAC GCTGAGCGGG CCAAGGACAT TCTTTGCCGC 
TTGGAACATA CAACATTGAG GAAGGTGACT GCCAACACAG CCATCTACTA TGACCCCAAC 
CCTCAGAGCA CAGTGGTGGC AGAAGATCAG GAGTGGGTGA ATGTGTACTA TGAGATGCCT 
GACTTTGATG TAGCCCGAAT ATCCCCCTGG CTGCTGCGTG TGGAACTGGA CCGAAAGCAC 
ATGACTGACC GGAAGCTTAC CATGGAACAG ATTGCTGAAA AAATCAATGC AGGCTTTGGC 
GATGATTTGA ATTGTATCTT CAATGATGAT AATGCAGAGA AGCTGGTCCT TCGTATCCGC 
ATCATGAACA GTGATGAAAA CAAGATGCAG GAGGAGGAAG AGGTGGTGGA TAAGATGGAT 
GATGATGTTT TCCTGCGGTG CATTGAGTCC AACATGCTGA CAGATATGAC CCTGCAGGGT 
ATTGAGCAGA TCAGCAAGGT ATACATGCAC TTACCTCAGA CAGACAACAA GAAGAAGATC 
ATCATCACAG AAGATGGGGA GTTCAAGGCC CTGCAAGAAT GGATCTTGGA GACAGATGGT 
GTGAGCCTGA TGCGGGTGCT GAGCGAGAAG GATGTAGACC CTGTGCGCAC TACGTCCAAT 
GACATCGTGG AGATCTTCAC GGTACTGGGC ATTGAGGCTG TGCGGAAGGC CCTGGAGCGA 
GAACTGTACC ATGTCATCTC CTTTGATGGT TCCTACGTCA ATTATCGGCA TTTGGCTCTT 
CTGTGTGACA CAATGACATG CCGTGGCCAC TTGATGGCCA TCACTCGTCA TGGAGTCAAC 
CGCCAGGACA CTGGACCACT CATGAAATGC TCCTTTGAGG AAACGGTGGA TGTCCTTATG 
GAAGCAGCTG CACATGGGGA GAGTGACCCC ATGAAGGGAG TCTCTGAGAA TATTATGCTG 
GGCCAGCTCG CTCCAGCTGG TACTGGCTGT TTTGACCTCC TGCTTGATGC TGAGAAATGC 
AAATACGGCA TGGAAATCCC CACCAATATC CCCGGACTGG GGGCCGCTGG ACCTACTGGC 
ATGTTCTTTG GCTCTGCACC CAGTCCGATG GGAGGAATCT CTCCTGCAAT GACACCCTGG 
AACCAGGGTG CAACTCCAGC CTATGGTGCC TGGTCCCCAA GTGTTGGGAG TGGGATGACC 
CCAGGAGCAG CTGGCTTCTC TCCCAGTGCT GCATCTGATG CCAGTGGCTT CAGCCCAGGT 
TACTCGCCTG CATGGTCTCC CACACCAGGC TCTCCAGGCT CCCCTGGACC CTCAAGCCCA 
TATATCCCCT CACCAGGTGG TGCTATGTCT CCCAGCTACT CACCAACATC ACCAGCATAT 
GAGCCGCGCT CCCCTGGGGG CTATACACCG CAGAGCCCCT CATACTCCCC TACTTCTCCT 
TCCTACTCCC CAACGTCTCC GTCTTATTCT CCAACCAGTC CCAACTATAG TCCTACCTCA 
CCTAGCTACT CCCCAACCTC TCCTAGCTAT TCCCCAACCT CTCCATCCTA CTCACCAACC 
TCTCCATCCT ACTCACCAAC CTCTCCCAGC TACTCCCCAA CCTCTCCCAG CTACTCCCCA 
ACATCACCCA GCTATTCTCC AACTTCTCCC AGCTACTCAC CAACATCTCC TAGCTATTCC 
CCAACATCTC CCAGCTACTC ACCAACCTCT CCAAGCTATT CTCCCACCTC CCCCAGTTAC 
TCACCGACAT CTCCAAGCTA CTCACCAACT TCTCCAAGTT ACTCACCAAC TTCCCCAAGT 
TACTCACCCA CTAGCCCTAA CTATTCCCCA ACTAGTCCCA ACTATACCCC AACCTCACCC 
AGCTACAGCC CAACCTCACC CAGCTACTCA CCTACTAGTC CAAACTATAC ACCTACCAGC 
CCTAACTACA GCCCAACCTC TCCAAGCTAT TCCCCAACCT CACCCAGTTA CTCTCCCACC 
TCACCCAGCT ACTCTCCCTC GAGCCCACGG TATACACCTC AGTCTCCAAC CTACACACCG 
AGTTCACCAA GCTACAGCCC TAGCTCGCCA AGCTACAGCC CTACTTCCCC CAAGTATACC 
CCAACTAGTC CTTCCTACAG TCCTAGCTCA CCAGAGTATA CCCCAACTTC TCCCAAATAC 
TCACCTACAA GCCCCAAATA TTCACCCACT TCTCCCAAGT ATTCTCCTAC CAGCCCCACT 
TACTCACCCA CCACCCCAAA ATACTCGCCA ACCTCTCCTA CATATTCACC AACCTCTCCA 
GTCTACACCC CGACCTCTCC CAAGTACTCC CCTACTAGTC CTACCTACTC CCCAACTTCT 
CCCAAGTACT CGCCCACCAG TCCCACCTAC TCACCCACCT CTCCCAAGGG CTCCACCTAC 
TCTCCCACTT CTCCTGGCTA CTCCCCCACC AGCCCCACCT ACAGCCTCAC CAGTCCAGCC 
ATCAGCCCAG AAGACAGCGA TGAGGAGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Polr2a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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