NRPB1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NRPB1
Entrez Gene ID	829734
Full Name	RNA polymerase II large subunit
Synonyms	F4B14.70,F4B14_70,RNA POLYMERASE II LARGE SUBUNIT,RNA polymerase II large subunit,RNA_POL_II_LS,RNA_POL_II_LSRNA_POL_II_LS,RPB1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_119746.4	NP_195305.2	RNA polymerase II large subunit
NM_001342374.1	NP_001329346.1	RNA polymerase II large subunit

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rpb1	NP_595673.1
Pan troglodytes (chimpanzee)	POLR2A	XP_511300.3
Homo sapiens (human)	POLR2A	NP_000928.1
Neurospora crassa	NCU02103	XP_964097.1
Oryza sativa (rice)	Os05g0151000	NP_001054670.2
Canis lupus familiaris (dog)	POLR2A	XP_857844.2
Bos taurus (cattle)	POLR2A	NP_001193242.1
Rattus norvegicus (Norway rat)	Polr2a	XP_343923.3
Caenorhabditis elegans (roundworm)	ama-1	NP_500523.4
Xenopus tropicalis (tropical clawed frog)	polr2a	XP_002943327.2
Macaca mulatta (Rhesus monkey)	POLR2A	XP_001118060.2
Mus musculus (house mouse)	Polr2a	NP_033115.1
Saccharomyces cerevisiae (baker's yeast)	RPO21	NP_010141.1
Arabidopsis thaliana (thale cress)	NRPB1	NP_195305.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007810	XP_317690.3
Magnaporthe oryzae (rice blast fungus)	MGG_04652	XP_003713642.1
Kluyveromyces lactis	KLLA0F05071g	XP_455310.1
Danio rerio (zebrafish)	polr2a	XP_005156282.1
Drosophila melanogaster (fruit fly)	RpII215	NP_511124.1
Eremothecium gossypii	AGOS_ADR086C	NP_984182.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Schüller C, Wambutt R, Murphy G, Volckaert G, Pohl T, Düsterhöft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, Müller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, Kötter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Blöcker H, Scharfe M, Grimm M, Löhnert TH, Dose S, de Haan M, Maarse A, Schäfer M, Müller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, Mündlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NRPB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NRPB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb32049	NM_119746.4 Latest version!	Arabidopsis thaliana RNA polymerase II large subunit (NRPB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAb32049	NM_001342374.1 Latest version!	Arabidopsis thaliana RNA polymerase II large subunit (NRPB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NRPB1 ( NM_119746.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_195305.2 Arabidopsis thaliana RNA polymerase II large subunit (NRPB1), mRNA.

Price & Availability↑

CloneID	OAb32049
Clone ID Related Accession (Same CDS sequence)	NM_119746.4 , NM_001342374.1
Accession Version	NM_119746.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549987200000
Organism	Arabidopsis thaliana(thale cress)
Product	RNA polymerase II large subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119746.3.

ATGGATACGA GGTTTCCGTT CTCTCCGGCC GAGGTCTCTA AAGTCCGGGT GGTCCAGTTT 
GGCATACTCA GCCCCGATGA GATCAGGCAA ATGTCTGTTA TACATGTTGA GCATAGTGAG 
ACGACCGAGA AGGGTAAACC TAAGGTGGGA GGATTGAGTG ATACCCGTCT TGGTACGATT 
GATCGGAAGG TGAAGTGTGA GACATGTATG GCTAATATGG CTGAGTGTCC GGGACATTTT 
GGCTATCTTG AGCTCGCTAA GCCAATGTAT CATGTCGGTT TTATGAAGAC AGTGTTAAGT 
ATCATGAGAT GTGTCTGTTT CAATTGCTCC AAGATTTTAG CTGATGAGGA GGAGCATAAG 
TTTAAGCAGG CTATGAAGAT CAAGAATCCT AAGAATAGGC TTAAGAAGAT TCTGGATGCC 
TGCAAAAACA AGACCAAATG TGATGGTGGT GATGACATTG ACGATGTCCA AAGCCACAGC 
ACGGATGAAC CAGTAAAAAA GAGCCGAGGT GGATGTGGTG CACAACAACC AAAACTGACT 
ATTGAGGGTA TGAAGATGAT TGCAGAATAC AAAATTCAAA GGAAGAAAAA TGATGAGCCA 
GATCAGCTTC CCGAGCCTGC AGAAAGGAAA CAGACACTTG GTGCTGATAG GGTTTTGAGT 
GTTTTGAAAA GGATTAGTGA CGCGGATTGT CAACTCCTAG GTTTCAACCC TAAGTTTGCT 
CGTCCTGACT GGATGATTCT TGAAGTCCTT CCTATTCCTC CACCCCCTGT CAGACCATCT 
GTAATGATGG ACGCCACTTC CAGGAGTGAG GATGACTTGA CCCATCAGCT AGCTATGATT 
ATTCGACACA ATGAAAACTT GAAAAGGCAG GAAAAAAATG GAGCGCCAGC TCATATTATA 
TCAGAGTTTA CACAACTCTT GCAGTTTCAT ATAGCTACGT ATTTTGATAA CGAGTTGCCT 
GGACAGCCAA GGGCTACTCA GAAATCAGGG AGGCCTATTA AATCAATATG TAGTAGGCTG 
AAGGCAAAGG AAGGCAGAAT CAGGGGTAAC TTGATGGGAA AACGTGTTGA TTTCTCGGCA 
CGTACTGTTA TTACTCCAGA TCCAACAATA AATATTGATG AACTTGGTGT TCCGTGGAGT 
ATTGCTCTGA ATCTCACATA CCCAGAAACA GTTACTCCCT ATAACATTGA AAGATTAAAG 
GAGCTTGTTG ATTATGGACC ACATCCTCCA CCTGGGAAGA CTGGAGCGAA ATATATCATA 
AGAGATGATG GCCAAAGACT AGATCTTCGG TATCTTAAGA AGAGCAGTGA TCAACATTTG 
GAACTTGGAT ACAAGGTGGA GCGGCATTTA CAGGATGGTG ATTTTGTTCT GTTTAATCGT 
CAACCAAGTC TGCACAAAAT GTCTATCATG GGTCACAGGA TTAGGATTAT GCCATATTCC 
ACTTTCCGTC TGAATTTGTC TGTCACGTCT CCGTACAATG CTGATTTTGA TGGGGATGAG 
ATGAACATGC ATGTACCACA ATCATTCGAG ACCAGAGCCG AGGTGTTAGA GCTGATGATG 
GTTCCTAAAT GTATTGTCTC CCCCCAGGCG AATCGTCCTG TGATGGGAAT TGTGCAGGAT 
ACCCTCTTGG GGTGCCGTAA AATTACAAAG AGAGATACTT TCATAGAGAA GGATGTATTC 
ATGAACACAC TGATGTGGTG GGAAGACTTC GATGGGAAAG TTCCGGCTCC TGCAATCTTG 
AAGCCTCGTC CTCTTTGGAC TGGCAAACAA GTTTTTAATC TTATCATACC AAAACAGATA 
AATCTGTTGA GGTACTCTGC TTGGCACGCA GATACAGAGA CTGGATTTAT AACTCCGGGG 
GATACTCAAG TGCGAATTGA AAGAGGGGAA CTTCTTGCCG GAACTCTTTG CAAAAAGACC 
CTTGGTACAT CTAATGGAAG TCTCGTGCAT GTCATTTGGG AAGAGGTTGG TCCTGATGCA 
GCTAGAAAAT TCCTCGGTCA TACTCAATGG CTTGTCAATT ACTGGCTTCT GCAGAATGGT 
TTTACCATCG GAATTGGTGA CACAATTGCC GATTCATCAA CAATGGAGAA AATTAATGAA 
ACTATTTCCA ATGCAAAAAC TGCTGTGAAA GATCTTATCC GGCAGTTCCA GGGAAAGGAA 
TTGGACCCTG AGCCTGGCCG AACTATGAGA GATACATTTG AGAACAGGGT TAACCAGGTT 
TTGAATAAAG CTCGTGATGA TGCTGGAAGT AGTGCTCAAA AGAGTTTAGC AGAAACCAAT 
AACCTTAAGG CCATGGTGAC AGCAGGATCC AAAGGAAGTT TCATCAATAT TTCTCAAATG 
ACAGCGTGTG TCGGTCAGCA AAATGTTGAA GGGAAGCGAA TTCCATTTGG ATTTGATGGG 
CGGACATTGC CACATTTCAC CAAAGATGAT TATGGTCCTG AAAGTCGTGG TTTTGTTGAG 
AATTCGTACC TGCGTGGCTT GACTCCTCAA GAGTTCTTTT TCCATGCTAT GGGAGGACGA 
GAAGGTCTTA TTGATACTGC TGTGAAGACA TCAGAAACTG GATACATTCA GAGGCGATTG 
GTAAAGGCTA TGGAGGATAT TATGGTTAAG TATGATGGGA CAGTCAGAAA CTCTTTGGGT 
GATGTTATTC AATTTCTCTA TGGAGAAGAT GGTATGGATG CTGTATGGAT AGAATCACAG 
AAGCTGGATT CCTTGAAAAT GAAGAAATCA GAGTTTGATA GGACTTTTAA GTATGAGATT 
GACGACGAAA ACTGGAATCC TACTTACCTA AGTGATGAAC ATCTTGAAGA CTTGAAGGGG 
ATTCGGGAGT TGCGTGATGT ATTCGATGCG GAATATTCGA AACTTGAGAC TGACAGATTC 
CAACTCGGGA CAGAAATTGC AACAAATGGT GATAGCACTT GGCCATTGCC TGTTAACATC 
AAGAGGCATA TCTGGAATGC GCAGAAGACT TTCAAAATTG ACTTGCGCAA AATTTCAGAT 
ATGCACCCTG TTGAAATTGT TGATGCTGTT GATAAACTAC AGGAGAGGCT GTTGGTTGTT 
CCTGGTGATG ATGCGTTGAG TGTGGAAGCA CAGAAAAACG CAACATTGTT CTTTAACATT 
TTGCTTCGCA GCACTCTTGC TAGTAAAAGA GTGTTGGAAG AATACAAGCT CAGCCGCGAG 
GCTTTTGAGT GGGTCATTGG TGAGATTGAA TCAAGGTTTT TACAATCGCT AGTGGCCCCA 
GGGGAAATGA TCGGTTGTGT TGCTGCTCAA TCAATTGGAG AACCTGCTAC GCAGATGACT 
CTGAATACCT TCCATTATGC TGGTGTCAGT GCAAAGAACG TTACGCTCGG AGTTCCCAGG 
TTGCGTGAAA TTATTAATGT AGCTAAGAGG ATCAAAACAC CATCCCTATC AGTCTATCTC 
ACTCCGGAAG CTAGCAAATC AAAAGAGGGG GCTAAGACTG TTCAGTGTGC TTTGGAGTAT 
ACTACTCTCA GGAGTGTTAC TCAAGCTACG GAAGTCTGGT ATGACCCAGA TCCAATGAGT 
ACAATAATTG AAGAGGACTT TGAATTTGTG AGGTCCTACT ATGAAATGCC AGATGAAGAT 
GTTTCCCCAG ATAAGATATC TCCGTGGCTA CTTCGTATAG AGTTGAATCG CGAGATGATG 
GTTGATAAGA AATTGAGTAT GGCGGATATT GCGGAGAAGA TCAACCTTGA GTTCGATGAT 
GACCTAACTT GCATATTCAA TGATGATAAT GCTCAAAAAC TGATCCTTCG AATTCGCATT 
ATGAACGATG AGGGCCCAAA GGGAGAGTTG CAAGATGAAT CGGCTGAAGA TGATGTTTTC 
CTCAAAAAGA TTGAGAGCAA CATGCTGACA GAAATGGCAC TCAGAGGTAT TCCAGACATC 
AACAAGGTTT TTATAAAACA GGTTAGAAAG AGCAGGTTTG ATGAGGAGGG AGGCTTCAAG 
ACATCTGAGG AGTGGATGTT GGATACAGAA GGTGTGAACC TCTTAGCTGT CATGTGTCAC 
GAAGATGTGG ATCCAAAGAG GACAACAAGC AATCACTTGA TTGAAATTAT TGAAGTTCTC 
GGAATTGAGG CAGTTCGTCG TGCTTTGCTT GATGAACTCC GTGTTGTGAT ATCCTTTGAT 
GGTTCTTATG TGAATTACCG TCATCTTGCC ATCTTGTGTG ATACTATGAC CTATCGCGGT 
CATCTGATGG CTATCACTCG ACACGGTATC AATAGAAATG ACACTGGGCC TCTGATGAGA 
TGCTCTTTTG AAGAAACAGT TGATATTCTG CTAGATGCTG CGGCTTATGC TGAGACAGAC 
TGCTTACGGG GTGTTACTGA GAATATAATG TTGGGTCAAC TTGCACCAAT TGGGACAGGA 
GATTGTGAGT TGTATCTGAA TGATGAGATG CTGAAGAATG CAATTGAACT TCAGCTCCCT 
AGCTATATGG ATGGTCTTGA ATTTGGAATG ACTCCTGCTC GTTCACCAGT GTCAGGCACT 
CCTTACCATG AAGGCATGAT GTCTCCAAAC TACCTGTTAA GTCCAAATAT GCGTTTATCC 
CCAATGTCAG ATGCACAGTT TTCTCCATAT GTTGGTGGAA TGGCCTTTTC GCCTTCTTCT 
TCTCCAGGAT ATAGTCCATC ATCGCCTGGA TACAGTCCTA CTTCTCCCGG TTACAGTCCA 
ACTTCGCCTG GATATAGCCC GACTTCTCCC GGTTACAGTC CAACTTCGCC TACCTACAGT 
CCCAGTTCTC CTGGCTATAG CCCAACAAGC CCTGCTTATT CTCCTACAAG TCCTTCCTAT 
TCTCCTACCT CTCCGAGCTA CAGCCCAACG TCTCCAAGCT ATAGCCCAAC GTCGCCAAGC 
TACAGCCCGA CATCTCCGAG CTACAGTCCT ACTTCCCCAA GTTACAGCCC GACTTCGCCT 
GCTTACAGCC CGACTTCACC TGCTTACAGC CCAACTTCAC CAGCATACAG CCCAACCTCT 
CCTTCTTACA GCCCAACTTC ACCTTCTTAC AGCCCAACAT CGCCTTCTTA CAGCCCTACT 
TCACCATCTT ACAGCCCAAC ATCTCCGTCT TACAGCCCTA CTTCACCCGC ATATAGCCCC 
ACATCTCCTG GCTACAGCCC TACTTCACCA AGTTACAGTC CAACATCACC AAGCTACGGT 
CCTACGTCTC CAAGCTACAA CCCTCAGTCT GCTAAATATA GCCCATCTAT AGCTTACTCT 
CCTAGCAATG CAAGACTATC ACCAGCTAGC CCCTACAGTC CTACATCTCC CAACTACAGC 
CCGACATCTC CATCATACTC ACCCACATCT CCATCTTATT CACCTTCAAG TCCAACATAC 
AGTCCCAGCA GCCCATACAG CTCAGGAGCA AGCCCAGACT ACAGCCCAAG CGCAGGCTAC 
TCGCCAACAC TTCCCGGTTA TTCACCGTCA TCAACGGGTC AGTATACCCC ACATGAGGGC 
GATAAAAAGG ACAAGACTGG AAAAAAAGAT GCCAGTAAGG ATGATAAAGG CAACCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_195305.2
CDS	237..5756
Translation	MDTRFPFSPAEVSKVRVVQFGILSPDEIRQMSVIHVEHSETTEKGKPKVGGLSDTRLGTIDRKVKCETCMANMAECPGHFGYLELAKPMYHVGFMKTVLSIMRCVCFNCSKILADEEEHKFKQAMKIKNPKNRLKKILDACKNKTKCDGGDDIDDVQSHSTDEPVKKSRGGCGAQQPKLTIEGMKMIAEYKIQRKKNDEPDQLPEPAERKQTLGADRVLSVLKRISDADCQLLGFNPKFARPDWMILEVLPIPPPPVRPSVMMDATSRSEDDLTHQLAMIIRHNENLKRQEKNGAPAHIISEFTQLLQFHIATYFDNELPGQPRATQKSGRPIKSICSRLKAKEGRIRGNLMGKRVDFSARTVITPDPTINIDELGVPWSIALNLTYPETVTPYNIERLKELVDYGPHPPPGKTGAKYIIRDDGQRLDLRYLKKSSDQHLELGYKVERHLQDGDFVLFNRQPSLHKMSIMGHRIRIMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSFETRAEVLELMMVPKCIVSPQANRPVMGIVQDTLLGCRKITKRDTFIEKDVFMNTLMWWEDFDGKVPAPAILKPRPLWTGKQVFNLIIPKQINLLRYSAWHADTETGFITPGDTQVRIERGELLAGTLCKKTLGTSNGSLVHVIWEEVGPDAARKFLGHTQWLVNYWLLQNGFTIGIGDTIADSSTMEKINETISNAKTAVKDLIRQFQGKELDPEPGRTMRDTFENRVNQVLNKARDDAGSSAQKSLAETNNLKAMVTAGSKGSFINISQMTACVGQQNVEGKRIPFGFDGRTLPHFTKDDYGPESRGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTSETGYIQRRLVKAMEDIMVKYDGTVRNSLGDVIQFLYGEDGMDAVWIESQKLDSLKMKKSEFDRTFKYEIDDENWNPTYLSDEHLEDLKGIRELRDVFDAEYSKLETDRFQLGTEIATNGDSTWPLPVNIKRHIWNAQKTFKIDLRKISDMHPVEIVDAVDKLQERLLVVPGDDALSVEAQKNATLFFNILLRSTLASKRVLEEYKLSREAFEWVIGEIESRFLQSLVAPGEMIGCVAAQSIGEPATQMTLNTFHYAGVSAKNVTLGVPRLREIINVAKRIKTPSLSVYLTPEASKSKEGAKTVQCALEYTTLRSVTQATEVWYDPDPMSTIIEEDFEFVRSYYEMPDEDVSPDKISPWLLRIELNREMMVDKKLSMADIAEKINLEFDDDLTCIFNDDNAQKLILRIRIMNDEGPKGELQDESAEDDVFLKKIESNMLTEMALRGIPDINKVFIKQVRKSRFDEEGGFKTSEEWMLDTEGVNLLAVMCHEDVDPKRTTSNHLIEIIEVLGIEAVRRALLDELRVVISFDGSYVNYRHLAILCDTMTYRGHLMAITRHGINRNDTGPLMRCSFEETVDILLDAAAYAETDCLRGVTENIMLGQLAPIGTGDCELYLNDEMLKNAIELQLPSYMDGLEFGMTPARSPVSGTPYHEGMMSPNYLLSPNMRLSPMSDAQFSPYVGGMAFSPSSSPGYSPSSPGYSPTSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYSPSIAYSPSNARLSPASPYSPTSPNYSPTSPSYSPTSPSYSPSSPTYSPSSPYSSGASPDYSPSAGYSPTLPGYSPSSTGQYTPHEGDKKDKTGKKDASKDDKGNP

Target ORF information:

RefSeq Version	NM_119746.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA polymerase II large subunit (NRPB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119746.4

ORF Insert Sequence:

ATGGATACGA GGTTTCCGTT CTCTCCGGCC GAGGTCTCTA AAGTCCGGGT GGTCCAGTTT 
GGCATACTCA GCCCCGATGA GATCAGGCAA ATGTCTGTTA TACATGTTGA GCATAGTGAG 
ACGACCGAGA AGGGTAAACC TAAGGTGGGA GGATTGAGTG ATACCCGTCT TGGTACGATT 
GATCGGAAGG TGAAGTGTGA GACATGTATG GCTAATATGG CTGAGTGTCC GGGACATTTT 
GGCTATCTTG AGCTCGCTAA GCCAATGTAT CATGTCGGTT TTATGAAGAC AGTGTTAAGT 
ATCATGAGAT GTGTCTGTTT CAATTGCTCC AAGATTTTAG CTGATGAGGA GGAGCATAAG 
TTTAAGCAGG CTATGAAGAT CAAGAATCCT AAGAATAGGC TTAAGAAGAT TCTGGATGCC 
TGCAAAAACA AGACCAAATG TGATGGTGGT GATGACATTG ACGATGTCCA AAGCCACAGC 
ACGGATGAAC CAGTAAAAAA GAGCCGAGGT GGATGTGGTG CACAACAACC AAAACTGACT 
ATTGAGGGTA TGAAGATGAT TGCAGAATAC AAAATTCAAA GGAAGAAAAA TGATGAGCCA 
GATCAGCTTC CCGAGCCTGC AGAAAGGAAA CAGACACTTG GTGCTGATAG GGTTTTGAGT 
GTTTTGAAAA GGATTAGTGA CGCGGATTGT CAACTCCTAG GTTTCAACCC TAAGTTTGCT 
CGTCCTGACT GGATGATTCT TGAAGTCCTT CCTATTCCTC CACCCCCTGT CAGACCATCT 
GTAATGATGG ACGCCACTTC CAGGAGTGAG GATGACTTGA CCCATCAGCT AGCTATGATT 
ATTCGACACA ATGAAAACTT GAAAAGGCAG GAAAAAAATG GAGCGCCAGC TCATATTATA 
TCAGAGTTTA CACAACTCTT GCAGTTTCAT ATAGCTACGT ATTTTGATAA CGAGTTGCCT 
GGACAGCCAA GGGCTACTCA GAAATCAGGG AGGCCTATTA AATCAATATG TAGTAGGCTG 
AAGGCAAAGG AAGGCAGAAT CAGGGGTAAC TTGATGGGAA AACGTGTTGA TTTCTCGGCA 
CGTACTGTTA TTACTCCAGA TCCAACAATA AATATTGATG AACTTGGTGT TCCGTGGAGT 
ATTGCTCTGA ATCTCACATA CCCAGAAACA GTTACTCCCT ATAACATTGA AAGATTAAAG 
GAGCTTGTTG ATTATGGACC ACATCCTCCA CCTGGGAAGA CTGGAGCGAA ATATATCATA 
AGAGATGATG GCCAAAGACT AGATCTTCGG TATCTTAAGA AGAGCAGTGA TCAACATTTG 
GAACTTGGAT ACAAGGTGGA GCGGCATTTA CAGGATGGTG ATTTTGTTCT GTTTAATCGT 
CAACCAAGTC TGCACAAAAT GTCTATCATG GGTCACAGGA TTAGGATTAT GCCATATTCC 
ACTTTCCGTC TGAATTTGTC TGTCACGTCT CCGTACAATG CTGATTTTGA TGGGGATGAG 
ATGAACATGC ATGTACCACA ATCATTCGAG ACCAGAGCCG AGGTGTTAGA GCTGATGATG 
GTTCCTAAAT GTATTGTCTC CCCCCAGGCG AATCGTCCTG TGATGGGAAT TGTGCAGGAT 
ACCCTCTTGG GGTGCCGTAA AATTACAAAG AGAGATACTT TCATAGAGAA GGATGTATTC 
ATGAACACAC TGATGTGGTG GGAAGACTTC GATGGGAAAG TTCCGGCTCC TGCAATCTTG 
AAGCCTCGTC CTCTTTGGAC TGGCAAACAA GTTTTTAATC TTATCATACC AAAACAGATA 
AATCTGTTGA GGTACTCTGC TTGGCACGCA GATACAGAGA CTGGATTTAT AACTCCGGGG 
GATACTCAAG TGCGAATTGA AAGAGGGGAA CTTCTTGCCG GAACTCTTTG CAAAAAGACC 
CTTGGTACAT CTAATGGAAG TCTCGTGCAT GTCATTTGGG AAGAGGTTGG TCCTGATGCA 
GCTAGAAAAT TCCTCGGTCA TACTCAATGG CTTGTCAATT ACTGGCTTCT GCAGAATGGT 
TTTACCATCG GAATTGGTGA CACAATTGCC GATTCATCAA CAATGGAGAA AATTAATGAA 
ACTATTTCCA ATGCAAAAAC TGCTGTGAAA GATCTTATCC GGCAGTTCCA GGGAAAGGAA 
TTGGACCCTG AGCCTGGCCG AACTATGAGA GATACATTTG AGAACAGGGT TAACCAGGTT 
TTGAATAAAG CTCGTGATGA TGCTGGAAGT AGTGCTCAAA AGAGTTTAGC AGAAACCAAT 
AACCTTAAGG CCATGGTGAC AGCAGGATCC AAAGGAAGTT TCATCAATAT TTCTCAAATG 
ACAGCGTGTG TCGGTCAGCA AAATGTTGAA GGGAAGCGAA TTCCATTTGG ATTTGATGGG 
CGGACATTGC CACATTTCAC CAAAGATGAT TATGGTCCTG AAAGTCGTGG TTTTGTTGAG 
AATTCGTACC TGCGTGGCTT GACTCCTCAA GAGTTCTTTT TCCATGCTAT GGGAGGACGA 
GAAGGTCTTA TTGATACTGC TGTGAAGACA TCAGAAACTG GATACATTCA GAGGCGATTG 
GTAAAGGCTA TGGAGGATAT TATGGTTAAG TATGATGGGA CAGTCAGAAA CTCTTTGGGT 
GATGTTATTC AATTTCTCTA TGGAGAAGAT GGTATGGATG CTGTATGGAT AGAATCACAG 
AAGCTGGATT CCTTGAAAAT GAAGAAATCA GAGTTTGATA GGACTTTTAA GTATGAGATT 
GACGACGAAA ACTGGAATCC TACTTACCTA AGTGATGAAC ATCTTGAAGA CTTGAAGGGG 
ATTCGGGAGT TGCGTGATGT ATTCGATGCG GAATATTCGA AACTTGAGAC TGACAGATTC 
CAACTCGGGA CAGAAATTGC AACAAATGGT GATAGCACTT GGCCATTGCC TGTTAACATC 
AAGAGGCATA TCTGGAATGC GCAGAAGACT TTCAAAATTG ACTTGCGCAA AATTTCAGAT 
ATGCACCCTG TTGAAATTGT TGATGCTGTT GATAAACTAC AGGAGAGGCT GTTGGTTGTT 
CCTGGTGATG ATGCGTTGAG TGTGGAAGCA CAGAAAAACG CAACATTGTT CTTTAACATT 
TTGCTTCGCA GCACTCTTGC TAGTAAAAGA GTGTTGGAAG AATACAAGCT CAGCCGCGAG 
GCTTTTGAGT GGGTCATTGG TGAGATTGAA TCAAGGTTTT TACAATCGCT AGTGGCCCCA 
GGGGAAATGA TCGGTTGTGT TGCTGCTCAA TCAATTGGAG AACCTGCTAC GCAGATGACT 
CTGAATACCT TCCATTATGC TGGTGTCAGT GCAAAGAACG TTACGCTCGG AGTTCCCAGG 
TTGCGTGAAA TTATTAATGT AGCTAAGAGG ATCAAAACAC CATCCCTATC AGTCTATCTC 
ACTCCGGAAG CTAGCAAATC AAAAGAGGGG GCTAAGACTG TTCAGTGTGC TTTGGAGTAT 
ACTACTCTCA GGAGTGTTAC TCAAGCTACG GAAGTCTGGT ATGACCCAGA TCCAATGAGT 
ACAATAATTG AAGAGGACTT TGAATTTGTG AGGTCCTACT ATGAAATGCC AGATGAAGAT 
GTTTCCCCAG ATAAGATATC TCCGTGGCTA CTTCGTATAG AGTTGAATCG CGAGATGATG 
GTTGATAAGA AATTGAGTAT GGCGGATATT GCGGAGAAGA TCAACCTTGA GTTCGATGAT 
GACCTAACTT GCATATTCAA TGATGATAAT GCTCAAAAAC TGATCCTTCG AATTCGCATT 
ATGAACGATG AGGGCCCAAA GGGAGAGTTG CAAGATGAAT CGGCTGAAGA TGATGTTTTC 
CTCAAAAAGA TTGAGAGCAA CATGCTGACA GAAATGGCAC TCAGAGGTAT TCCAGACATC 
AACAAGGTTT TTATAAAACA GGTTAGAAAG AGCAGGTTTG ATGAGGAGGG AGGCTTCAAG 
ACATCTGAGG AGTGGATGTT GGATACAGAA GGTGTGAACC TCTTAGCTGT CATGTGTCAC 
GAAGATGTGG ATCCAAAGAG GACAACAAGC AATCACTTGA TTGAAATTAT TGAAGTTCTC 
GGAATTGAGG CAGTTCGTCG TGCTTTGCTT GATGAACTCC GTGTTGTGAT ATCCTTTGAT 
GGTTCTTATG TGAATTACCG TCATCTTGCC ATCTTGTGTG ATACTATGAC CTATCGCGGT 
CATCTGATGG CTATCACTCG ACACGGTATC AATAGAAATG ACACTGGGCC TCTGATGAGA 
TGCTCTTTTG AAGAAACAGT TGATATTCTG CTAGATGCTG CGGCTTATGC TGAGACAGAC 
TGCTTACGGG GTGTTACTGA GAATATAATG TTGGGTCAAC TTGCACCAAT TGGGACAGGA 
GATTGTGAGT TGTATCTGAA TGATGAGATG CTGAAGAATG CAATTGAACT TCAGCTCCCT 
AGCTATATGG ATGGTCTTGA ATTTGGAATG ACTCCTGCTC GTTCACCAGT GTCAGGCACT 
CCTTACCATG AAGGCATGAT GTCTCCAAAC TACCTGTTAA GTCCAAATAT GCGTTTATCC 
CCAATGTCAG ATGCACAGTT TTCTCCATAT GTTGGTGGAA TGGCCTTTTC GCCTTCTTCT 
TCTCCAGGAT ATAGTCCATC ATCGCCTGGA TACAGTCCTA CTTCTCCCGG TTACAGTCCA 
ACTTCGCCTG GATATAGCCC GACTTCTCCC GGTTACAGTC CAACTTCGCC TACCTACAGT 
CCCAGTTCTC CTGGCTATAG CCCAACAAGC CCTGCTTATT CTCCTACAAG TCCTTCCTAT 
TCTCCTACCT CTCCGAGCTA CAGCCCAACG TCTCCAAGCT ATAGCCCAAC GTCGCCAAGC 
TACAGCCCGA CATCTCCGAG CTACAGTCCT ACTTCCCCAA GTTACAGCCC GACTTCGCCT 
GCTTACAGCC CGACTTCACC TGCTTACAGC CCAACTTCAC CAGCATACAG CCCAACCTCT 
CCTTCTTACA GCCCAACTTC ACCTTCTTAC AGCCCAACAT CGCCTTCTTA CAGCCCTACT 
TCACCATCTT ACAGCCCAAC ATCTCCGTCT TACAGCCCTA CTTCACCCGC ATATAGCCCC 
ACATCTCCTG GCTACAGCCC TACTTCACCA AGTTACAGTC CAACATCACC AAGCTACGGT 
CCTACGTCTC CAAGCTACAA CCCTCAGTCT GCTAAATATA GCCCATCTAT AGCTTACTCT 
CCTAGCAATG CAAGACTATC ACCAGCTAGC CCCTACAGTC CTACATCTCC CAACTACAGC 
CCGACATCTC CATCATACTC ACCCACATCT CCATCTTATT CACCTTCAAG TCCAACATAC 
AGTCCCAGCA GCCCATACAG CTCAGGAGCA AGCCCAGACT ACAGCCCAAG CGCAGGCTAC 
TCGCCAACAC TTCCCGGTTA TTCACCGTCA TCAACGGGTC AGTATACCCC ACATGAGGGC 
GATAAAAAGG ACAAGACTGG AAAAAAAGAT GCCAGTAAGG ATGATAAAGG CAACCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NRPB1 ( NM_001342374.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001329346.1 Arabidopsis thaliana RNA polymerase II large subunit (NRPB1), mRNA.

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CloneID	OAb32049
Clone ID Related Accession (Same CDS sequence)	NM_119746.4 , NM_001342374.1
Accession Version	NM_001342374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1549987200000
Organism	Arabidopsis thaliana(thale cress)
Product	RNA polymerase II large subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGATACGA GGTTTCCGTT CTCTCCGGCC GAGGTCTCTA AAGTCCGGGT GGTCCAGTTT 
GGCATACTCA GCCCCGATGA GATCAGGCAA ATGTCTGTTA TACATGTTGA GCATAGTGAG 
ACGACCGAGA AGGGTAAACC TAAGGTGGGA GGATTGAGTG ATACCCGTCT TGGTACGATT 
GATCGGAAGG TGAAGTGTGA GACATGTATG GCTAATATGG CTGAGTGTCC GGGACATTTT 
GGCTATCTTG AGCTCGCTAA GCCAATGTAT CATGTCGGTT TTATGAAGAC AGTGTTAAGT 
ATCATGAGAT GTGTCTGTTT CAATTGCTCC AAGATTTTAG CTGATGAGGA GGAGCATAAG 
TTTAAGCAGG CTATGAAGAT CAAGAATCCT AAGAATAGGC TTAAGAAGAT TCTGGATGCC 
TGCAAAAACA AGACCAAATG TGATGGTGGT GATGACATTG ACGATGTCCA AAGCCACAGC 
ACGGATGAAC CAGTAAAAAA GAGCCGAGGT GGATGTGGTG CACAACAACC AAAACTGACT 
ATTGAGGGTA TGAAGATGAT TGCAGAATAC AAAATTCAAA GGAAGAAAAA TGATGAGCCA 
GATCAGCTTC CCGAGCCTGC AGAAAGGAAA CAGACACTTG GTGCTGATAG GGTTTTGAGT 
GTTTTGAAAA GGATTAGTGA CGCGGATTGT CAACTCCTAG GTTTCAACCC TAAGTTTGCT 
CGTCCTGACT GGATGATTCT TGAAGTCCTT CCTATTCCTC CACCCCCTGT CAGACCATCT 
GTAATGATGG ACGCCACTTC CAGGAGTGAG GATGACTTGA CCCATCAGCT AGCTATGATT 
ATTCGACACA ATGAAAACTT GAAAAGGCAG GAAAAAAATG GAGCGCCAGC TCATATTATA 
TCAGAGTTTA CACAACTCTT GCAGTTTCAT ATAGCTACGT ATTTTGATAA CGAGTTGCCT 
GGACAGCCAA GGGCTACTCA GAAATCAGGG AGGCCTATTA AATCAATATG TAGTAGGCTG 
AAGGCAAAGG AAGGCAGAAT CAGGGGTAAC TTGATGGGAA AACGTGTTGA TTTCTCGGCA 
CGTACTGTTA TTACTCCAGA TCCAACAATA AATATTGATG AACTTGGTGT TCCGTGGAGT 
ATTGCTCTGA ATCTCACATA CCCAGAAACA GTTACTCCCT ATAACATTGA AAGATTAAAG 
GAGCTTGTTG ATTATGGACC ACATCCTCCA CCTGGGAAGA CTGGAGCGAA ATATATCATA 
AGAGATGATG GCCAAAGACT AGATCTTCGG TATCTTAAGA AGAGCAGTGA TCAACATTTG 
GAACTTGGAT ACAAGGTGGA GCGGCATTTA CAGGATGGTG ATTTTGTTCT GTTTAATCGT 
CAACCAAGTC TGCACAAAAT GTCTATCATG GGTCACAGGA TTAGGATTAT GCCATATTCC 
ACTTTCCGTC TGAATTTGTC TGTCACGTCT CCGTACAATG CTGATTTTGA TGGGGATGAG 
ATGAACATGC ATGTACCACA ATCATTCGAG ACCAGAGCCG AGGTGTTAGA GCTGATGATG 
GTTCCTAAAT GTATTGTCTC CCCCCAGGCG AATCGTCCTG TGATGGGAAT TGTGCAGGAT 
ACCCTCTTGG GGTGCCGTAA AATTACAAAG AGAGATACTT TCATAGAGAA GGATGTATTC 
ATGAACACAC TGATGTGGTG GGAAGACTTC GATGGGAAAG TTCCGGCTCC TGCAATCTTG 
AAGCCTCGTC CTCTTTGGAC TGGCAAACAA GTTTTTAATC TTATCATACC AAAACAGATA 
AATCTGTTGA GGTACTCTGC TTGGCACGCA GATACAGAGA CTGGATTTAT AACTCCGGGG 
GATACTCAAG TGCGAATTGA AAGAGGGGAA CTTCTTGCCG GAACTCTTTG CAAAAAGACC 
CTTGGTACAT CTAATGGAAG TCTCGTGCAT GTCATTTGGG AAGAGGTTGG TCCTGATGCA 
GCTAGAAAAT TCCTCGGTCA TACTCAATGG CTTGTCAATT ACTGGCTTCT GCAGAATGGT 
TTTACCATCG GAATTGGTGA CACAATTGCC GATTCATCAA CAATGGAGAA AATTAATGAA 
ACTATTTCCA ATGCAAAAAC TGCTGTGAAA GATCTTATCC GGCAGTTCCA GGGAAAGGAA 
TTGGACCCTG AGCCTGGCCG AACTATGAGA GATACATTTG AGAACAGGGT TAACCAGGTT 
TTGAATAAAG CTCGTGATGA TGCTGGAAGT AGTGCTCAAA AGAGTTTAGC AGAAACCAAT 
AACCTTAAGG CCATGGTGAC AGCAGGATCC AAAGGAAGTT TCATCAATAT TTCTCAAATG 
ACAGCGTGTG TCGGTCAGCA AAATGTTGAA GGGAAGCGAA TTCCATTTGG ATTTGATGGG 
CGGACATTGC CACATTTCAC CAAAGATGAT TATGGTCCTG AAAGTCGTGG TTTTGTTGAG 
AATTCGTACC TGCGTGGCTT GACTCCTCAA GAGTTCTTTT TCCATGCTAT GGGAGGACGA 
GAAGGTCTTA TTGATACTGC TGTGAAGACA TCAGAAACTG GATACATTCA GAGGCGATTG 
GTAAAGGCTA TGGAGGATAT TATGGTTAAG TATGATGGGA CAGTCAGAAA CTCTTTGGGT 
GATGTTATTC AATTTCTCTA TGGAGAAGAT GGTATGGATG CTGTATGGAT AGAATCACAG 
AAGCTGGATT CCTTGAAAAT GAAGAAATCA GAGTTTGATA GGACTTTTAA GTATGAGATT 
GACGACGAAA ACTGGAATCC TACTTACCTA AGTGATGAAC ATCTTGAAGA CTTGAAGGGG 
ATTCGGGAGT TGCGTGATGT ATTCGATGCG GAATATTCGA AACTTGAGAC TGACAGATTC 
CAACTCGGGA CAGAAATTGC AACAAATGGT GATAGCACTT GGCCATTGCC TGTTAACATC 
AAGAGGCATA TCTGGAATGC GCAGAAGACT TTCAAAATTG ACTTGCGCAA AATTTCAGAT 
ATGCACCCTG TTGAAATTGT TGATGCTGTT GATAAACTAC AGGAGAGGCT GTTGGTTGTT 
CCTGGTGATG ATGCGTTGAG TGTGGAAGCA CAGAAAAACG CAACATTGTT CTTTAACATT 
TTGCTTCGCA GCACTCTTGC TAGTAAAAGA GTGTTGGAAG AATACAAGCT CAGCCGCGAG 
GCTTTTGAGT GGGTCATTGG TGAGATTGAA TCAAGGTTTT TACAATCGCT AGTGGCCCCA 
GGGGAAATGA TCGGTTGTGT TGCTGCTCAA TCAATTGGAG AACCTGCTAC GCAGATGACT 
CTGAATACCT TCCATTATGC TGGTGTCAGT GCAAAGAACG TTACGCTCGG AGTTCCCAGG 
TTGCGTGAAA TTATTAATGT AGCTAAGAGG ATCAAAACAC CATCCCTATC AGTCTATCTC 
ACTCCGGAAG CTAGCAAATC AAAAGAGGGG GCTAAGACTG TTCAGTGTGC TTTGGAGTAT 
ACTACTCTCA GGAGTGTTAC TCAAGCTACG GAAGTCTGGT ATGACCCAGA TCCAATGAGT 
ACAATAATTG AAGAGGACTT TGAATTTGTG AGGTCCTACT ATGAAATGCC AGATGAAGAT 
GTTTCCCCAG ATAAGATATC TCCGTGGCTA CTTCGTATAG AGTTGAATCG CGAGATGATG 
GTTGATAAGA AATTGAGTAT GGCGGATATT GCGGAGAAGA TCAACCTTGA GTTCGATGAT 
GACCTAACTT GCATATTCAA TGATGATAAT GCTCAAAAAC TGATCCTTCG AATTCGCATT 
ATGAACGATG AGGGCCCAAA GGGAGAGTTG CAAGATGAAT CGGCTGAAGA TGATGTTTTC 
CTCAAAAAGA TTGAGAGCAA CATGCTGACA GAAATGGCAC TCAGAGGTAT TCCAGACATC 
AACAAGGTTT TTATAAAACA GGTTAGAAAG AGCAGGTTTG ATGAGGAGGG AGGCTTCAAG 
ACATCTGAGG AGTGGATGTT GGATACAGAA GGTGTGAACC TCTTAGCTGT CATGTGTCAC 
GAAGATGTGG ATCCAAAGAG GACAACAAGC AATCACTTGA TTGAAATTAT TGAAGTTCTC 
GGAATTGAGG CAGTTCGTCG TGCTTTGCTT GATGAACTCC GTGTTGTGAT ATCCTTTGAT 
GGTTCTTATG TGAATTACCG TCATCTTGCC ATCTTGTGTG ATACTATGAC CTATCGCGGT 
CATCTGATGG CTATCACTCG ACACGGTATC AATAGAAATG ACACTGGGCC TCTGATGAGA 
TGCTCTTTTG AAGAAACAGT TGATATTCTG CTAGATGCTG CGGCTTATGC TGAGACAGAC 
TGCTTACGGG GTGTTACTGA GAATATAATG TTGGGTCAAC TTGCACCAAT TGGGACAGGA 
GATTGTGAGT TGTATCTGAA TGATGAGATG CTGAAGAATG CAATTGAACT TCAGCTCCCT 
AGCTATATGG ATGGTCTTGA ATTTGGAATG ACTCCTGCTC GTTCACCAGT GTCAGGCACT 
CCTTACCATG AAGGCATGAT GTCTCCAAAC TACCTGTTAA GTCCAAATAT GCGTTTATCC 
CCAATGTCAG ATGCACAGTT TTCTCCATAT GTTGGTGGAA TGGCCTTTTC GCCTTCTTCT 
TCTCCAGGAT ATAGTCCATC ATCGCCTGGA TACAGTCCTA CTTCTCCCGG TTACAGTCCA 
ACTTCGCCTG GATATAGCCC GACTTCTCCC GGTTACAGTC CAACTTCGCC TACCTACAGT 
CCCAGTTCTC CTGGCTATAG CCCAACAAGC CCTGCTTATT CTCCTACAAG TCCTTCCTAT 
TCTCCTACCT CTCCGAGCTA CAGCCCAACG TCTCCAAGCT ATAGCCCAAC GTCGCCAAGC 
TACAGCCCGA CATCTCCGAG CTACAGTCCT ACTTCCCCAA GTTACAGCCC GACTTCGCCT 
GCTTACAGCC CGACTTCACC TGCTTACAGC CCAACTTCAC CAGCATACAG CCCAACCTCT 
CCTTCTTACA GCCCAACTTC ACCTTCTTAC AGCCCAACAT CGCCTTCTTA CAGCCCTACT 
TCACCATCTT ACAGCCCAAC ATCTCCGTCT TACAGCCCTA CTTCACCCGC ATATAGCCCC 
ACATCTCCTG GCTACAGCCC TACTTCACCA AGTTACAGTC CAACATCACC AAGCTACGGT 
CCTACGTCTC CAAGCTACAA CCCTCAGTCT GCTAAATATA GCCCATCTAT AGCTTACTCT 
CCTAGCAATG CAAGACTATC ACCAGCTAGC CCCTACAGTC CTACATCTCC CAACTACAGC 
CCGACATCTC CATCATACTC ACCCACATCT CCATCTTATT CACCTTCAAG TCCAACATAC 
AGTCCCAGCA GCCCATACAG CTCAGGAGCA AGCCCAGACT ACAGCCCAAG CGCAGGCTAC 
TCGCCAACAC TTCCCGGTTA TTCACCGTCA TCAACGGGTC AGTATACCCC ACATGAGGGC 
GATAAAAAGG ACAAGACTGG AAAAAAAGAT GCCAGTAAGG ATGATAAAGG CAACCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001329346.1
CDS	237..5756
Translation	MDTRFPFSPAEVSKVRVVQFGILSPDEIRQMSVIHVEHSETTEKGKPKVGGLSDTRLGTIDRKVKCETCMANMAECPGHFGYLELAKPMYHVGFMKTVLSIMRCVCFNCSKILADEEEHKFKQAMKIKNPKNRLKKILDACKNKTKCDGGDDIDDVQSHSTDEPVKKSRGGCGAQQPKLTIEGMKMIAEYKIQRKKNDEPDQLPEPAERKQTLGADRVLSVLKRISDADCQLLGFNPKFARPDWMILEVLPIPPPPVRPSVMMDATSRSEDDLTHQLAMIIRHNENLKRQEKNGAPAHIISEFTQLLQFHIATYFDNELPGQPRATQKSGRPIKSICSRLKAKEGRIRGNLMGKRVDFSARTVITPDPTINIDELGVPWSIALNLTYPETVTPYNIERLKELVDYGPHPPPGKTGAKYIIRDDGQRLDLRYLKKSSDQHLELGYKVERHLQDGDFVLFNRQPSLHKMSIMGHRIRIMPYSTFRLNLSVTSPYNADFDGDEMNMHVPQSFETRAEVLELMMVPKCIVSPQANRPVMGIVQDTLLGCRKITKRDTFIEKDVFMNTLMWWEDFDGKVPAPAILKPRPLWTGKQVFNLIIPKQINLLRYSAWHADTETGFITPGDTQVRIERGELLAGTLCKKTLGTSNGSLVHVIWEEVGPDAARKFLGHTQWLVNYWLLQNGFTIGIGDTIADSSTMEKINETISNAKTAVKDLIRQFQGKELDPEPGRTMRDTFENRVNQVLNKARDDAGSSAQKSLAETNNLKAMVTAGSKGSFINISQMTACVGQQNVEGKRIPFGFDGRTLPHFTKDDYGPESRGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTSETGYIQRRLVKAMEDIMVKYDGTVRNSLGDVIQFLYGEDGMDAVWIESQKLDSLKMKKSEFDRTFKYEIDDENWNPTYLSDEHLEDLKGIRELRDVFDAEYSKLETDRFQLGTEIATNGDSTWPLPVNIKRHIWNAQKTFKIDLRKISDMHPVEIVDAVDKLQERLLVVPGDDALSVEAQKNATLFFNILLRSTLASKRVLEEYKLSREAFEWVIGEIESRFLQSLVAPGEMIGCVAAQSIGEPATQMTLNTFHYAGVSAKNVTLGVPRLREIINVAKRIKTPSLSVYLTPEASKSKEGAKTVQCALEYTTLRSVTQATEVWYDPDPMSTIIEEDFEFVRSYYEMPDEDVSPDKISPWLLRIELNREMMVDKKLSMADIAEKINLEFDDDLTCIFNDDNAQKLILRIRIMNDEGPKGELQDESAEDDVFLKKIESNMLTEMALRGIPDINKVFIKQVRKSRFDEEGGFKTSEEWMLDTEGVNLLAVMCHEDVDPKRTTSNHLIEIIEVLGIEAVRRALLDELRVVISFDGSYVNYRHLAILCDTMTYRGHLMAITRHGINRNDTGPLMRCSFEETVDILLDAAAYAETDCLRGVTENIMLGQLAPIGTGDCELYLNDEMLKNAIELQLPSYMDGLEFGMTPARSPVSGTPYHEGMMSPNYLLSPNMRLSPMSDAQFSPYVGGMAFSPSSSPGYSPSSPGYSPTSPGYSPTSPGYSPTSPGYSPTSPTYSPSSPGYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPAYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPGYSPTSPSYSPTSPSYGPTSPSYNPQSAKYSPSIAYSPSNARLSPASPYSPTSPNYSPTSPSYSPTSPSYSPSSPTYSPSSPYSSGASPDYSPSAGYSPTLPGYSPSSTGQYTPHEGDKKDKTGKKDASKDDKGNP

Target ORF information:

RefSeq Version	NM_001342374.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana RNA polymerase II large subunit (NRPB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342374.1

ORF Insert Sequence:

ATGGATACGA GGTTTCCGTT CTCTCCGGCC GAGGTCTCTA AAGTCCGGGT GGTCCAGTTT 
GGCATACTCA GCCCCGATGA GATCAGGCAA ATGTCTGTTA TACATGTTGA GCATAGTGAG 
ACGACCGAGA AGGGTAAACC TAAGGTGGGA GGATTGAGTG ATACCCGTCT TGGTACGATT 
GATCGGAAGG TGAAGTGTGA GACATGTATG GCTAATATGG CTGAGTGTCC GGGACATTTT 
GGCTATCTTG AGCTCGCTAA GCCAATGTAT CATGTCGGTT TTATGAAGAC AGTGTTAAGT 
ATCATGAGAT GTGTCTGTTT CAATTGCTCC AAGATTTTAG CTGATGAGGA GGAGCATAAG 
TTTAAGCAGG CTATGAAGAT CAAGAATCCT AAGAATAGGC TTAAGAAGAT TCTGGATGCC 
TGCAAAAACA AGACCAAATG TGATGGTGGT GATGACATTG ACGATGTCCA AAGCCACAGC 
ACGGATGAAC CAGTAAAAAA GAGCCGAGGT GGATGTGGTG CACAACAACC AAAACTGACT 
ATTGAGGGTA TGAAGATGAT TGCAGAATAC AAAATTCAAA GGAAGAAAAA TGATGAGCCA 
GATCAGCTTC CCGAGCCTGC AGAAAGGAAA CAGACACTTG GTGCTGATAG GGTTTTGAGT 
GTTTTGAAAA GGATTAGTGA CGCGGATTGT CAACTCCTAG GTTTCAACCC TAAGTTTGCT 
CGTCCTGACT GGATGATTCT TGAAGTCCTT CCTATTCCTC CACCCCCTGT CAGACCATCT 
GTAATGATGG ACGCCACTTC CAGGAGTGAG GATGACTTGA CCCATCAGCT AGCTATGATT 
ATTCGACACA ATGAAAACTT GAAAAGGCAG GAAAAAAATG GAGCGCCAGC TCATATTATA 
TCAGAGTTTA CACAACTCTT GCAGTTTCAT ATAGCTACGT ATTTTGATAA CGAGTTGCCT 
GGACAGCCAA GGGCTACTCA GAAATCAGGG AGGCCTATTA AATCAATATG TAGTAGGCTG 
AAGGCAAAGG AAGGCAGAAT CAGGGGTAAC TTGATGGGAA AACGTGTTGA TTTCTCGGCA 
CGTACTGTTA TTACTCCAGA TCCAACAATA AATATTGATG AACTTGGTGT TCCGTGGAGT 
ATTGCTCTGA ATCTCACATA CCCAGAAACA GTTACTCCCT ATAACATTGA AAGATTAAAG 
GAGCTTGTTG ATTATGGACC ACATCCTCCA CCTGGGAAGA CTGGAGCGAA ATATATCATA 
AGAGATGATG GCCAAAGACT AGATCTTCGG TATCTTAAGA AGAGCAGTGA TCAACATTTG 
GAACTTGGAT ACAAGGTGGA GCGGCATTTA CAGGATGGTG ATTTTGTTCT GTTTAATCGT 
CAACCAAGTC TGCACAAAAT GTCTATCATG GGTCACAGGA TTAGGATTAT GCCATATTCC 
ACTTTCCGTC TGAATTTGTC TGTCACGTCT CCGTACAATG CTGATTTTGA TGGGGATGAG 
ATGAACATGC ATGTACCACA ATCATTCGAG ACCAGAGCCG AGGTGTTAGA GCTGATGATG 
GTTCCTAAAT GTATTGTCTC CCCCCAGGCG AATCGTCCTG TGATGGGAAT TGTGCAGGAT 
ACCCTCTTGG GGTGCCGTAA AATTACAAAG AGAGATACTT TCATAGAGAA GGATGTATTC 
ATGAACACAC TGATGTGGTG GGAAGACTTC GATGGGAAAG TTCCGGCTCC TGCAATCTTG 
AAGCCTCGTC CTCTTTGGAC TGGCAAACAA GTTTTTAATC TTATCATACC AAAACAGATA 
AATCTGTTGA GGTACTCTGC TTGGCACGCA GATACAGAGA CTGGATTTAT AACTCCGGGG 
GATACTCAAG TGCGAATTGA AAGAGGGGAA CTTCTTGCCG GAACTCTTTG CAAAAAGACC 
CTTGGTACAT CTAATGGAAG TCTCGTGCAT GTCATTTGGG AAGAGGTTGG TCCTGATGCA 
GCTAGAAAAT TCCTCGGTCA TACTCAATGG CTTGTCAATT ACTGGCTTCT GCAGAATGGT 
TTTACCATCG GAATTGGTGA CACAATTGCC GATTCATCAA CAATGGAGAA AATTAATGAA 
ACTATTTCCA ATGCAAAAAC TGCTGTGAAA GATCTTATCC GGCAGTTCCA GGGAAAGGAA 
TTGGACCCTG AGCCTGGCCG AACTATGAGA GATACATTTG AGAACAGGGT TAACCAGGTT 
TTGAATAAAG CTCGTGATGA TGCTGGAAGT AGTGCTCAAA AGAGTTTAGC AGAAACCAAT 
AACCTTAAGG CCATGGTGAC AGCAGGATCC AAAGGAAGTT TCATCAATAT TTCTCAAATG 
ACAGCGTGTG TCGGTCAGCA AAATGTTGAA GGGAAGCGAA TTCCATTTGG ATTTGATGGG 
CGGACATTGC CACATTTCAC CAAAGATGAT TATGGTCCTG AAAGTCGTGG TTTTGTTGAG 
AATTCGTACC TGCGTGGCTT GACTCCTCAA GAGTTCTTTT TCCATGCTAT GGGAGGACGA 
GAAGGTCTTA TTGATACTGC TGTGAAGACA TCAGAAACTG GATACATTCA GAGGCGATTG 
GTAAAGGCTA TGGAGGATAT TATGGTTAAG TATGATGGGA CAGTCAGAAA CTCTTTGGGT 
GATGTTATTC AATTTCTCTA TGGAGAAGAT GGTATGGATG CTGTATGGAT AGAATCACAG 
AAGCTGGATT CCTTGAAAAT GAAGAAATCA GAGTTTGATA GGACTTTTAA GTATGAGATT 
GACGACGAAA ACTGGAATCC TACTTACCTA AGTGATGAAC ATCTTGAAGA CTTGAAGGGG 
ATTCGGGAGT TGCGTGATGT ATTCGATGCG GAATATTCGA AACTTGAGAC TGACAGATTC 
CAACTCGGGA CAGAAATTGC AACAAATGGT GATAGCACTT GGCCATTGCC TGTTAACATC 
AAGAGGCATA TCTGGAATGC GCAGAAGACT TTCAAAATTG ACTTGCGCAA AATTTCAGAT 
ATGCACCCTG TTGAAATTGT TGATGCTGTT GATAAACTAC AGGAGAGGCT GTTGGTTGTT 
CCTGGTGATG ATGCGTTGAG TGTGGAAGCA CAGAAAAACG CAACATTGTT CTTTAACATT 
TTGCTTCGCA GCACTCTTGC TAGTAAAAGA GTGTTGGAAG AATACAAGCT CAGCCGCGAG 
GCTTTTGAGT GGGTCATTGG TGAGATTGAA TCAAGGTTTT TACAATCGCT AGTGGCCCCA 
GGGGAAATGA TCGGTTGTGT TGCTGCTCAA TCAATTGGAG AACCTGCTAC GCAGATGACT 
CTGAATACCT TCCATTATGC TGGTGTCAGT GCAAAGAACG TTACGCTCGG AGTTCCCAGG 
TTGCGTGAAA TTATTAATGT AGCTAAGAGG ATCAAAACAC CATCCCTATC AGTCTATCTC 
ACTCCGGAAG CTAGCAAATC AAAAGAGGGG GCTAAGACTG TTCAGTGTGC TTTGGAGTAT 
ACTACTCTCA GGAGTGTTAC TCAAGCTACG GAAGTCTGGT ATGACCCAGA TCCAATGAGT 
ACAATAATTG AAGAGGACTT TGAATTTGTG AGGTCCTACT ATGAAATGCC AGATGAAGAT 
GTTTCCCCAG ATAAGATATC TCCGTGGCTA CTTCGTATAG AGTTGAATCG CGAGATGATG 
GTTGATAAGA AATTGAGTAT GGCGGATATT GCGGAGAAGA TCAACCTTGA GTTCGATGAT 
GACCTAACTT GCATATTCAA TGATGATAAT GCTCAAAAAC TGATCCTTCG AATTCGCATT 
ATGAACGATG AGGGCCCAAA GGGAGAGTTG CAAGATGAAT CGGCTGAAGA TGATGTTTTC 
CTCAAAAAGA TTGAGAGCAA CATGCTGACA GAAATGGCAC TCAGAGGTAT TCCAGACATC 
AACAAGGTTT TTATAAAACA GGTTAGAAAG AGCAGGTTTG ATGAGGAGGG AGGCTTCAAG 
ACATCTGAGG AGTGGATGTT GGATACAGAA GGTGTGAACC TCTTAGCTGT CATGTGTCAC 
GAAGATGTGG ATCCAAAGAG GACAACAAGC AATCACTTGA TTGAAATTAT TGAAGTTCTC 
GGAATTGAGG CAGTTCGTCG TGCTTTGCTT GATGAACTCC GTGTTGTGAT ATCCTTTGAT 
GGTTCTTATG TGAATTACCG TCATCTTGCC ATCTTGTGTG ATACTATGAC CTATCGCGGT 
CATCTGATGG CTATCACTCG ACACGGTATC AATAGAAATG ACACTGGGCC TCTGATGAGA 
TGCTCTTTTG AAGAAACAGT TGATATTCTG CTAGATGCTG CGGCTTATGC TGAGACAGAC 
TGCTTACGGG GTGTTACTGA GAATATAATG TTGGGTCAAC TTGCACCAAT TGGGACAGGA 
GATTGTGAGT TGTATCTGAA TGATGAGATG CTGAAGAATG CAATTGAACT TCAGCTCCCT 
AGCTATATGG ATGGTCTTGA ATTTGGAATG ACTCCTGCTC GTTCACCAGT GTCAGGCACT 
CCTTACCATG AAGGCATGAT GTCTCCAAAC TACCTGTTAA GTCCAAATAT GCGTTTATCC 
CCAATGTCAG ATGCACAGTT TTCTCCATAT GTTGGTGGAA TGGCCTTTTC GCCTTCTTCT 
TCTCCAGGAT ATAGTCCATC ATCGCCTGGA TACAGTCCTA CTTCTCCCGG TTACAGTCCA 
ACTTCGCCTG GATATAGCCC GACTTCTCCC GGTTACAGTC CAACTTCGCC TACCTACAGT 
CCCAGTTCTC CTGGCTATAG CCCAACAAGC CCTGCTTATT CTCCTACAAG TCCTTCCTAT 
TCTCCTACCT CTCCGAGCTA CAGCCCAACG TCTCCAAGCT ATAGCCCAAC GTCGCCAAGC 
TACAGCCCGA CATCTCCGAG CTACAGTCCT ACTTCCCCAA GTTACAGCCC GACTTCGCCT 
GCTTACAGCC CGACTTCACC TGCTTACAGC CCAACTTCAC CAGCATACAG CCCAACCTCT 
CCTTCTTACA GCCCAACTTC ACCTTCTTAC AGCCCAACAT CGCCTTCTTA CAGCCCTACT 
TCACCATCTT ACAGCCCAAC ATCTCCGTCT TACAGCCCTA CTTCACCCGC ATATAGCCCC 
ACATCTCCTG GCTACAGCCC TACTTCACCA AGTTACAGTC CAACATCACC AAGCTACGGT 
CCTACGTCTC CAAGCTACAA CCCTCAGTCT GCTAAATATA GCCCATCTAT AGCTTACTCT 
CCTAGCAATG CAAGACTATC ACCAGCTAGC CCCTACAGTC CTACATCTCC CAACTACAGC 
CCGACATCTC CATCATACTC ACCCACATCT CCATCTTATT CACCTTCAAG TCCAACATAC 
AGTCCCAGCA GCCCATACAG CTCAGGAGCA AGCCCAGACT ACAGCCCAAG CGCAGGCTAC 
TCGCCAACAC TTCCCGGTTA TTCACCGTCA TCAACGGGTC AGTATACCCC ACATGAGGGC 
GATAAAAAGG ACAAGACTGG AAAAAAAGAT GCCAGTAAGG ATGATAAAGG CAACCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Schüller C,Wambutt R,Murphy G,Volckaert G,Pohl T,Düsterhöft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,Müller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,Kötter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Blöcker H,Scharfe M,Grimm M,Löhnert TH,Dose S,de Haan M,Maarse A,Schäfer M,Müller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,Mündlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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NRPB1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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