POLR2A cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR2A
Entrez Gene ID	479483
Full Name	RNA polymerase II subunit A
General protein information	Preferred Names RNA polymerase II subunit A Names DNA-directed RNA polymerase II subunit RPB1 220 kDa DNA-directed RNA polymerase polypeptide A polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_852751.4	XP_857844.3	DNA-directed RNA polymerase II subunit RPB1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rpb1	NP_595673.1
Pan troglodytes (chimpanzee)	POLR2A	XP_511300.3
Homo sapiens (human)	POLR2A	NP_000928.1
Neurospora crassa	NCU02103	XP_964097.1
Oryza sativa (rice)	Os05g0151000	NP_001054670.2
Canis lupus familiaris (dog)	POLR2A	XP_857844.2
Bos taurus (cattle)	POLR2A	NP_001193242.1
Rattus norvegicus (Norway rat)	Polr2a	XP_343923.3
Caenorhabditis elegans (roundworm)	ama-1	NP_500523.4
Xenopus tropicalis (tropical clawed frog)	polr2a	XP_002943327.2
Macaca mulatta (Rhesus monkey)	POLR2A	XP_001118060.2
Mus musculus (house mouse)	Polr2a	NP_033115.1
Saccharomyces cerevisiae (baker's yeast)	RPO21	NP_010141.1
Arabidopsis thaliana (thale cress)	NRPB1	NP_195305.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007810	XP_317690.3
Magnaporthe oryzae (rice blast fungus)	MGG_04652	XP_003713642.1
Kluyveromyces lactis	KLLA0F05071g	XP_455310.1
Danio rerio (zebrafish)	polr2a	XP_005156282.1
Drosophila melanogaster (fruit fly)	RpII215	NP_511124.1
Eremothecium gossypii	AGOS_ADR086C	NP_984182.2

Gene Ontology

The following POLR2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe36416	XM_852751.4 Latest version!	Canis lupus familiaris RNA polymerase II subunit A (POLR2A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR2A ( XM_852751.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_857844.3 Canis lupus familiaris RNA polymerase II subunit A (POLR2A), mRNA.

Price & Availability↑

CloneID	OCe36416
Clone ID Related Accession (Same CDS sequence)	XM_852751.4
Accession Version	XM_852751.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	DNA-directed RNA polymerase II subunit RPB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_852751.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGGG GTGGCCCCCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGCGG 
GTGCAGTTCG GAGTCCTCAG TCCGGATGAA CTGAAACGAA TGTCTGTGAC AGAGGGCGGT 
ATCAAATACC CCGAGACGAC GGAGGGAGGC CGCCCCAAGC TTGGGGGGCT GATGGACCCA 
AGGCAGGGGG TGATTGAGCG GACTGGCCGC TGCCAAACCT GTGCAGGAAA CATGACAGAG 
TGTCCTGGCC ACTTTGGCCA CATCGAGCTG GCCAAGCCCG TGTTTCACGT GGGCTTCCTG 
GTGAAGACGA TGAAGGTTTT GCGCTGTGTC TGCTTCTTCT GCTCCAAGTT GCTTGTGGAC 
TCTAACAACC CAAAGATCAA GGACATCCTG GCCAAGTCCA AGGGGCAGCC CAAGAAGCGG 
CTCACCCACG TGTATGACCT GTGCAAGGGC AAGAACATCT GCGAAGGCGG TGAGGAGATG 
GATAACAAGT TCGGGGTGGA GCAGCCCGAG GGTGATGAGG ACTTGACCAA AGAAAAGGGC 
CATGGGGGCT GCGGACGATA CCAGCCCCGC ATCCGGCGCT CAGGCCTGGA GCTGTATGCC 
GAGTGGAAGC ATGTCAATGA GGACTCCCAG GAGAAGAAGA TCCTGCTGAG TCCTGAGCGA 
GTACATGAAA TCTTCAAGCG CATCTCAGAC GAAGAGTGTT TTGTGCTGGG CATGGAGCCC 
CGCTACGCCC GGCCTGAGTG GATGATTGTC ACTGTGCTGC CCGTGCCCCC GCTGTCTGTG 
CGGCCTGCCG TGGTGATGCA GGGCTCTGCC CGCAACCAGG ATGACTTGAC CCACAAGCTG 
GCGGATATCG TGAAGATCAA CAATCAGTTG CGGCGCAATG AGCAAAACGG TGCAGCGGCC 
CACGTCATCG CAGAGGATGT GAAGCTCCTC CAGTTCCATG TGGCCACGAT GGTGGACAAT 
GAGCTGCCTG GCCTGCCCCG GGCCATGCAG AAGTCTGGGC GTCCCCTCAA GTCCCTGAAG 
CAGCGGTTGA AGGGCAAGGA AGGCCGAGTC CGAGGGAACC TGATGGGCAA GCGAGTGGAC 
TTCTCAGCCC GCACCGTCAT CACTCCCGAC CCCAACCTTT CCATCGACCA GGTCGGTGTG 
CCTCGCTCCA TTGCTGCCAA CATGACCTTT GCAGAGATCG TCACGCCCTT CAACATCGAC 
AGACTTCAGG AATTGGTGCG CAGGGGGAAC AGCCAGTACC CAGGGGCCAA GTACATCATC 
CGAGACAATG GCGATCGCAT TGACCTGCGT TTCCACCCAA AGCCCAGTGA CCTTCATTTG 
CAGACTGGCT ATAAGGTGGA ACGGCACATG TGCGATGGAG ACATTGTCAT CTTCAACCGG 
CAGCCGACTT TGCACAAGAT GTCCATGATG GGGCATCGGG TCCGCATCCT CCCCTGGTCT 
ACTTTTCGCT TAAATCTGAG TGTAACAACT CCATACAATG CTGACTTTGA TGGGGATGAG 
ATGAACTTGC ACCTGCCACA GTCTCTGGAG ACTCGGGCAG AGATCCAGGA GCTCGCCATG 
GTGCCGCGCA TGATTGTCAC CCCCCAGAGC AATCGCCCCG TCATGGGCAT CGTGCAGGAC 
ACGCTCACAG CTGTGCGCAA ATTCACAAAG AGAGATGTCT TCCTGGAGCG GGGGGAAGTT 
ATGAACCTTT TGATGTTCTT GTCCACGTGG GATGGGAAGG TCCCGCAGCC GGCCATCCTG 
AAGCCTCGTC CCCTGTGGAC AGGCAAACAG ATCTTCTCCC TCATCATACC AGGCCACATC 
AACTGCATCC GCACCCACAG CACCCATCCC GACGACGAGG ACAGTGGTCC TTACAAACAC 
ATCTCTCCAG GGGACACCAA GGTGGTAGTG GAGAACGGGG AGCTGATCAT GGGCATCCTG 
TGTAAGAAAT CTCTGGGTAC GTCAGCCGGC TCGCTGGTCC ACATTTCTTA CCTGGAGATG 
GGCCATGACG TCACTCGTCT CTTCTACTCC AACATTCAGA CAGTCATTAA CAACTGGCTT 
CTCATCGAGG GTCATACCAT TGGCATTGGG GACTCCATTG CGGACTCAAA GACCTACCAG 
GACATTCAGA ACACTATTAA GAAGGCTAAG CAGGATGTGA TAGAGGTCAT TGAGAAGGCT 
CATAACAATG AGCTGGAGCC CACCCCCGGG AACACTCTGC GGCAGACCTT TGAGAACCAG 
GTGAACCGCA TTCTCAACGA TGCCCGAGAT AAGACTGGCT CCTCTGCCCA GAAGTCCTTG 
TCTGAGTACA ACAACTTTAA GTCCATGGTC GTGTCTGGGG CCAAAGGTTC CAAGATCAAC 
ATCTCCCAGG TCATCGCTGT AGTCGGGCAG CAGAACGTGG AGGGCAAGCG GATCCCATTT 
GGATTCAAGC ACCGGACCCT GCCTCATTTC ATCAAGGATG ACTACGGGCC AGAGAGCCGA 
GGCTTTGTGG AGAATTCCTA CCTGGCCGGC CTCACGCCTA CCGAGTTCTT CTTCCATGCC 
ATGGGGGGTC GCGAGGGGCT TATCGACACG GCCGTCAAGA CTGCTGAAAC TGGGTACATC 
CAGCGGCGGC TCATCAAGTC CATGGAGTCG GTGATGGTGA AGTATGATGC TACTGTGCGA 
AACTCCATCA ACCAGGTGGT GCAGCTGCGC TATGGCGAGG ACGGCCTGGC TGGCGAGAGC 
GTCGAGTTCC AGAACCTGGC TACCCTTAAG CCATCCAACA AGGCTTTTGA GAAGAAGTTC 
CGCTTTGACT ACACAAACGA GAGGGCCCTG CGGCGCACTC TGCAGGAGGA CCTGGTGAAG 
GACGTGCTGA GCAATGCGCA CATTCAGAAC GAGTTAGAGC GAGAATTCGA GCGCATGCGT 
GAGGACAGGG AGGTGCTCAG GGTCATCTTC CCGACTGGCG ACAGCAAGGT CGTCCTCCCC 
TGTAACCTGC TGCGTATGAT CTGGAACGCT CAGAAAATCT TCCACATCAA CCCTCGCCTT 
CCCTCCGACT TGCACCCCAT CAAGGTGGTA GAGGGAGTCA AGGAATTGAG CAAGAAACTC 
GTAATTGTGA ACGGGGATGA CCCCCTGAGC AGGCAAGCTC AGGAGAATGC CACACTTCTC 
TTCAACATCC ACTTGCGCTC CACACTGTGC TCCCGCCGCA TGGCTGAGGA GTTCCGGCTC 
AGCGGGGAGG CCTTCGACTG GCTGCTTGGA GAGATCGAGT CCAAGTTCAA TCAAGCCATT 
GCCCATCCTG GGGAGATGGT GGGAGCCCTG GCTGCACAGT CCCTTGGAGA ACCTGCCACC 
CAGATGACCT TGAATACCTT TCACTATGCT GGTGTGTCTG CCAAAAACGT GACCCTGGGT 
GTGCCCCGAC TCAAGGAACT CATCAACATT TCCAAGAAGC CAAAGACGCC TTCACTTACT 
GTCTTCCTGC TGGGCCAGTC TGCTCGAGAT GCTGAGAGAG CCAAGGACAT TCTGTGCCGT 
CTGGAACATA CAACATTGAG GAAGGTGACC GCCAACACAG CCATCTACTA CGACCCCAAC 
CCCCAGAGCA CTGTCGTGGC AGAGGATCAG GAATGGGTGA ATGTCTACTA TGAGATGCCG 
GACTTTGACG TGGCCCGAAT CTCGCCCTGG CTCTTGCGGG TGGAGCTGGA CCGGAAGCAC 
ATGACAGACC GGAAGCTGAC CATGGAGCAG ATTGCTGAGA AGATCAATGC TGGTTTCGGC 
GATGACTTGA ACTGCATCTT TAACGACGAC AACGCTGAGA AGCTGGTGCT CCGAATCCGC 
ATCATGAACA GCGATGAAAA CAAGATGCAA GAGGAGGAAG AGGTGGTGGA CAAAATGGAT 
GATGACGTCT TCCTGCGCTG CATCGAGTCC AATATGCTGA CTGACATGAC CCTGCAGGGC 
ATTGAGCAGA TTAGCAAGGT GTATATGCAC CTGCCACAGA CGGACAACAA GAAGAAGATC 
ATTATCACCG AAGACGGGGA ATTCAAGGCT CTGCAGGAGT GGATCCTTGA GACGGACGGC 
GTGAGCCTGA TGCGGGTGCT GAGCGAGAAG GACGTGGACC CTGTCCGTAC CACGTCCAAC 
GACATTGTGG AGATCTTCAC GGTGCTGGGC ATTGAAGCTG TGCGGAAGGC CCTGGAGCGG 
GAGCTGTACC ACGTCATCTC CTTTGACGGC TCCTACGTCA ATTACCGCCA CTTGGCACTC 
CTGTGTGATA CCATGACCTG CCGGGGCCAC TTGATGGCCA TCACCCGCCA TGGTGTCAAT 
CGCCAGGATA CTGGGCCTCT CATGAAGTGC TCCTTTGAGG AAACGGTGGA CGTGCTTATG 
GAAGCAGCTG CTCACGGAGA GAGTGACCCC ATGAAGGGGG TGTCTGAGAA CATCATGCTG 
GGTCAGCTGG CCCCGGCTGG CACCGGCTGC TTTGATCTCC TGCTCGATGC TGAGAAGTGC 
AAGTATGGCA TGGAGATCCC CACCAACATC CCTGGCCTGG GGGCTGCCGG ACCCACTGGC 
ATGTTCTTTG GATCGGCACC TAGTCCCATG GGAGGAATTT CTCCTGCCAT GACTCCCTGG 
AACCAGGGGG CAACTCCAGC CTATGGTGCC TGGTCCCCTA GTGTCGGAAG CGGAATGACG 
CCGGGGGCAG CCGGCTTCTC TCCCAGTGCT GCGTCAGATG CCAGTGGCTT CAGCCCAGGC 
TACTCTCCTG CCTGGTCTCC CACGCCGGGC TCGCCTGGGT CACCGGGCCC CTCCAGCCCC 
TACATCCCCT CACCAGGTGG CGCTATGTCT CCCAGCTACT CACCGACATC GCCTGCCTAT 
GAGCCTCGCT CCCCTGGGGG CTATACACCC CAGAGTCCCT CCTATTCCCC TACTTCGCCC 
TCCTACTCCC CCACCTCTCC GTCCTACTCT CCAACTAGTC CCAACTATAG TCCCACATCA 
CCCAGCTACT CCCCAACTTC TCCCAGCTAC TCCCCAACTT CTCCCAGCTA CTCCCCAACT 
TCTCCCAGCT ACTCCCCAAC TTCTCCCAGC TACTCCCCAA CCTCTCCCAG CTACTCCCCC 
ACCTCGCCCA GCTATTCCCC CACCTCGCCC AGCTACTCCC CCACCTCGCC CAGCTACTCT 
CCCACCTCAC CCAGCTACTC CCCCACCTCT CCCAGCTACT CCCCCACATC CCCAAGCTAC 
TCGCCCACAT CCCCAAGCTA CTCGCCCACA TCCCCAAGCT ATTCCCCGAC ATCTCCGAGC 
TACTCGCCAA CCAGCCCTAA CTATTCTCCA TCCAGGCCCA ATTATACCCC GACGTCACCC 
AGCTATAGCC CAACGTCACC CAGCTACTCC CCAACTAGTC CCAACTACAC GCCCATGAGC 
CCTAACTACA GCCCAACGTC TCCGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_857844.3
CDS	422..5791
Translation	MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDVTRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPSRPNYTPTSPSYSPTSPSYSPTSPNYTPMSPNYSPTSPN

Target ORF information:

RefSeq Version	XM_852751.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris RNA polymerase II subunit A (POLR2A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_852751.4

ORF Insert Sequence:

ATGCACGGGG GTGGCCCCCC CTCCGGGGAC AGCGCATGCC CGCTGCGCAC CATCAAGCGG 
GTGCAGTTCG GAGTCCTCAG TCCGGATGAA CTGAAACGAA TGTCTGTGAC AGAGGGCGGT 
ATCAAATACC CCGAGACGAC GGAGGGAGGC CGCCCCAAGC TTGGGGGGCT GATGGACCCA 
AGGCAGGGGG TGATTGAGCG GACTGGCCGC TGCCAAACCT GTGCAGGAAA CATGACAGAG 
TGTCCTGGCC ACTTTGGCCA CATCGAGCTG GCCAAGCCCG TGTTTCACGT GGGCTTCCTG 
GTGAAGACGA TGAAGGTTTT GCGCTGTGTC TGCTTCTTCT GCTCCAAGTT GCTTGTGGAC 
TCTAACAACC CAAAGATCAA GGACATCCTG GCCAAGTCCA AGGGGCAGCC CAAGAAGCGG 
CTCACCCACG TGTATGACCT GTGCAAGGGC AAGAACATCT GCGAAGGCGG TGAGGAGATG 
GATAACAAGT TCGGGGTGGA GCAGCCCGAG GGTGATGAGG ACTTGACCAA AGAAAAGGGC 
CATGGGGGCT GCGGACGATA CCAGCCCCGC ATCCGGCGCT CAGGCCTGGA GCTGTATGCC 
GAGTGGAAGC ATGTCAATGA GGACTCCCAG GAGAAGAAGA TCCTGCTGAG TCCTGAGCGA 
GTACATGAAA TCTTCAAGCG CATCTCAGAC GAAGAGTGTT TTGTGCTGGG CATGGAGCCC 
CGCTACGCCC GGCCTGAGTG GATGATTGTC ACTGTGCTGC CCGTGCCCCC GCTGTCTGTG 
CGGCCTGCCG TGGTGATGCA GGGCTCTGCC CGCAACCAGG ATGACTTGAC CCACAAGCTG 
GCGGATATCG TGAAGATCAA CAATCAGTTG CGGCGCAATG AGCAAAACGG TGCAGCGGCC 
CACGTCATCG CAGAGGATGT GAAGCTCCTC CAGTTCCATG TGGCCACGAT GGTGGACAAT 
GAGCTGCCTG GCCTGCCCCG GGCCATGCAG AAGTCTGGGC GTCCCCTCAA GTCCCTGAAG 
CAGCGGTTGA AGGGCAAGGA AGGCCGAGTC CGAGGGAACC TGATGGGCAA GCGAGTGGAC 
TTCTCAGCCC GCACCGTCAT CACTCCCGAC CCCAACCTTT CCATCGACCA GGTCGGTGTG 
CCTCGCTCCA TTGCTGCCAA CATGACCTTT GCAGAGATCG TCACGCCCTT CAACATCGAC 
AGACTTCAGG AATTGGTGCG CAGGGGGAAC AGCCAGTACC CAGGGGCCAA GTACATCATC 
CGAGACAATG GCGATCGCAT TGACCTGCGT TTCCACCCAA AGCCCAGTGA CCTTCATTTG 
CAGACTGGCT ATAAGGTGGA ACGGCACATG TGCGATGGAG ACATTGTCAT CTTCAACCGG 
CAGCCGACTT TGCACAAGAT GTCCATGATG GGGCATCGGG TCCGCATCCT CCCCTGGTCT 
ACTTTTCGCT TAAATCTGAG TGTAACAACT CCATACAATG CTGACTTTGA TGGGGATGAG 
ATGAACTTGC ACCTGCCACA GTCTCTGGAG ACTCGGGCAG AGATCCAGGA GCTCGCCATG 
GTGCCGCGCA TGATTGTCAC CCCCCAGAGC AATCGCCCCG TCATGGGCAT CGTGCAGGAC 
ACGCTCACAG CTGTGCGCAA ATTCACAAAG AGAGATGTCT TCCTGGAGCG GGGGGAAGTT 
ATGAACCTTT TGATGTTCTT GTCCACGTGG GATGGGAAGG TCCCGCAGCC GGCCATCCTG 
AAGCCTCGTC CCCTGTGGAC AGGCAAACAG ATCTTCTCCC TCATCATACC AGGCCACATC 
AACTGCATCC GCACCCACAG CACCCATCCC GACGACGAGG ACAGTGGTCC TTACAAACAC 
ATCTCTCCAG GGGACACCAA GGTGGTAGTG GAGAACGGGG AGCTGATCAT GGGCATCCTG 
TGTAAGAAAT CTCTGGGTAC GTCAGCCGGC TCGCTGGTCC ACATTTCTTA CCTGGAGATG 
GGCCATGACG TCACTCGTCT CTTCTACTCC AACATTCAGA CAGTCATTAA CAACTGGCTT 
CTCATCGAGG GTCATACCAT TGGCATTGGG GACTCCATTG CGGACTCAAA GACCTACCAG 
GACATTCAGA ACACTATTAA GAAGGCTAAG CAGGATGTGA TAGAGGTCAT TGAGAAGGCT 
CATAACAATG AGCTGGAGCC CACCCCCGGG AACACTCTGC GGCAGACCTT TGAGAACCAG 
GTGAACCGCA TTCTCAACGA TGCCCGAGAT AAGACTGGCT CCTCTGCCCA GAAGTCCTTG 
TCTGAGTACA ACAACTTTAA GTCCATGGTC GTGTCTGGGG CCAAAGGTTC CAAGATCAAC 
ATCTCCCAGG TCATCGCTGT AGTCGGGCAG CAGAACGTGG AGGGCAAGCG GATCCCATTT 
GGATTCAAGC ACCGGACCCT GCCTCATTTC ATCAAGGATG ACTACGGGCC AGAGAGCCGA 
GGCTTTGTGG AGAATTCCTA CCTGGCCGGC CTCACGCCTA CCGAGTTCTT CTTCCATGCC 
ATGGGGGGTC GCGAGGGGCT TATCGACACG GCCGTCAAGA CTGCTGAAAC TGGGTACATC 
CAGCGGCGGC TCATCAAGTC CATGGAGTCG GTGATGGTGA AGTATGATGC TACTGTGCGA 
AACTCCATCA ACCAGGTGGT GCAGCTGCGC TATGGCGAGG ACGGCCTGGC TGGCGAGAGC 
GTCGAGTTCC AGAACCTGGC TACCCTTAAG CCATCCAACA AGGCTTTTGA GAAGAAGTTC 
CGCTTTGACT ACACAAACGA GAGGGCCCTG CGGCGCACTC TGCAGGAGGA CCTGGTGAAG 
GACGTGCTGA GCAATGCGCA CATTCAGAAC GAGTTAGAGC GAGAATTCGA GCGCATGCGT 
GAGGACAGGG AGGTGCTCAG GGTCATCTTC CCGACTGGCG ACAGCAAGGT CGTCCTCCCC 
TGTAACCTGC TGCGTATGAT CTGGAACGCT CAGAAAATCT TCCACATCAA CCCTCGCCTT 
CCCTCCGACT TGCACCCCAT CAAGGTGGTA GAGGGAGTCA AGGAATTGAG CAAGAAACTC 
GTAATTGTGA ACGGGGATGA CCCCCTGAGC AGGCAAGCTC AGGAGAATGC CACACTTCTC 
TTCAACATCC ACTTGCGCTC CACACTGTGC TCCCGCCGCA TGGCTGAGGA GTTCCGGCTC 
AGCGGGGAGG CCTTCGACTG GCTGCTTGGA GAGATCGAGT CCAAGTTCAA TCAAGCCATT 
GCCCATCCTG GGGAGATGGT GGGAGCCCTG GCTGCACAGT CCCTTGGAGA ACCTGCCACC 
CAGATGACCT TGAATACCTT TCACTATGCT GGTGTGTCTG CCAAAAACGT GACCCTGGGT 
GTGCCCCGAC TCAAGGAACT CATCAACATT TCCAAGAAGC CAAAGACGCC TTCACTTACT 
GTCTTCCTGC TGGGCCAGTC TGCTCGAGAT GCTGAGAGAG CCAAGGACAT TCTGTGCCGT 
CTGGAACATA CAACATTGAG GAAGGTGACC GCCAACACAG CCATCTACTA CGACCCCAAC 
CCCCAGAGCA CTGTCGTGGC AGAGGATCAG GAATGGGTGA ATGTCTACTA TGAGATGCCG 
GACTTTGACG TGGCCCGAAT CTCGCCCTGG CTCTTGCGGG TGGAGCTGGA CCGGAAGCAC 
ATGACAGACC GGAAGCTGAC CATGGAGCAG ATTGCTGAGA AGATCAATGC TGGTTTCGGC 
GATGACTTGA ACTGCATCTT TAACGACGAC AACGCTGAGA AGCTGGTGCT CCGAATCCGC 
ATCATGAACA GCGATGAAAA CAAGATGCAA GAGGAGGAAG AGGTGGTGGA CAAAATGGAT 
GATGACGTCT TCCTGCGCTG CATCGAGTCC AATATGCTGA CTGACATGAC CCTGCAGGGC 
ATTGAGCAGA TTAGCAAGGT GTATATGCAC CTGCCACAGA CGGACAACAA GAAGAAGATC 
ATTATCACCG AAGACGGGGA ATTCAAGGCT CTGCAGGAGT GGATCCTTGA GACGGACGGC 
GTGAGCCTGA TGCGGGTGCT GAGCGAGAAG GACGTGGACC CTGTCCGTAC CACGTCCAAC 
GACATTGTGG AGATCTTCAC GGTGCTGGGC ATTGAAGCTG TGCGGAAGGC CCTGGAGCGG 
GAGCTGTACC ACGTCATCTC CTTTGACGGC TCCTACGTCA ATTACCGCCA CTTGGCACTC 
CTGTGTGATA CCATGACCTG CCGGGGCCAC TTGATGGCCA TCACCCGCCA TGGTGTCAAT 
CGCCAGGATA CTGGGCCTCT CATGAAGTGC TCCTTTGAGG AAACGGTGGA CGTGCTTATG 
GAAGCAGCTG CTCACGGAGA GAGTGACCCC ATGAAGGGGG TGTCTGAGAA CATCATGCTG 
GGTCAGCTGG CCCCGGCTGG CACCGGCTGC TTTGATCTCC TGCTCGATGC TGAGAAGTGC 
AAGTATGGCA TGGAGATCCC CACCAACATC CCTGGCCTGG GGGCTGCCGG ACCCACTGGC 
ATGTTCTTTG GATCGGCACC TAGTCCCATG GGAGGAATTT CTCCTGCCAT GACTCCCTGG 
AACCAGGGGG CAACTCCAGC CTATGGTGCC TGGTCCCCTA GTGTCGGAAG CGGAATGACG 
CCGGGGGCAG CCGGCTTCTC TCCCAGTGCT GCGTCAGATG CCAGTGGCTT CAGCCCAGGC 
TACTCTCCTG CCTGGTCTCC CACGCCGGGC TCGCCTGGGT CACCGGGCCC CTCCAGCCCC 
TACATCCCCT CACCAGGTGG CGCTATGTCT CCCAGCTACT CACCGACATC GCCTGCCTAT 
GAGCCTCGCT CCCCTGGGGG CTATACACCC CAGAGTCCCT CCTATTCCCC TACTTCGCCC 
TCCTACTCCC CCACCTCTCC GTCCTACTCT CCAACTAGTC CCAACTATAG TCCCACATCA 
CCCAGCTACT CCCCAACTTC TCCCAGCTAC TCCCCAACTT CTCCCAGCTA CTCCCCAACT 
TCTCCCAGCT ACTCCCCAAC TTCTCCCAGC TACTCCCCAA CCTCTCCCAG CTACTCCCCC 
ACCTCGCCCA GCTATTCCCC CACCTCGCCC AGCTACTCCC CCACCTCGCC CAGCTACTCT 
CCCACCTCAC CCAGCTACTC CCCCACCTCT CCCAGCTACT CCCCCACATC CCCAAGCTAC 
TCGCCCACAT CCCCAAGCTA CTCGCCCACA TCCCCAAGCT ATTCCCCGAC ATCTCCGAGC 
TACTCGCCAA CCAGCCCTAA CTATTCTCCA TCCAGGCCCA ATTATACCCC GACGTCACCC 
AGCTATAGCC CAACGTCACC CAGCTACTCC CCAACTAGTC CCAACTACAC GCCCATGAGC 
CCTAACTACA GCCCAACGTC TCCGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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POLR2A cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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