Pole cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pole
Entrez Gene ID	304573
Full Name	DNA polymerase epsilon, catalytic subunit
Synonyms	POLE1
General protein information	Preferred Names DNA polymerase epsilon, catalytic subunit Names DNA polymerase epsilon catalytic subunit A DNA-directed DNA polymerase epsilon polymerase (DNA directed), epsilon, catalytic subunit polymerase (DNA) epsilon, catalytic subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16

mRNA and Protein(s)

mRNA	Protein	Name
XM_008769335.2	XP_008767557.1	DNA polymerase epsilon catalytic subunit A isoform X1
XM_006249562.3	XP_006249624.1	DNA polymerase epsilon catalytic subunit A isoform X2
NM_001107152.2	NP_001100622.2	DNA polymerase epsilon catalytic subunit A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

DNA polymerase δ and ζ switch by sharing accessory subunits of DNA polymerase δ.
Baranovskiy AG, Lada AG, Siebler HM, Zhang Y, Pavlov YI, Tahirov TH
The Journal of biological chemistry287(21)17281-7(2012 May)

DNA polymerases and repair synthesis in NER in human cells.
Lehmann AR
DNA repair10(7)730-3(2011 Jul)

Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells.
Ogi T, Limsirichaikul S, Overmeer RM, Volker M, Takenaka K, Cloney R, Nakazawa Y, Niimi A, Miki Y, Jaspers NG, Mullenders LH, Yamashita S, Fousteri MI, Lehmann AR
Molecular cell37(5)714-27(2010 Mar)

Distinctive activities of DNA polymerases during human DNA replication.
Rytkönen AK, Vaara M, Nethanel T, Kaufmann G, Sormunen R, Läärä E, Nasheuer HP, Rahmeh A, Lee MY, Syväoja JE, Pospiech H
The FEBS journal273(13)2984-3001(2006 Jul)

DNA polymerase epsilon - more than a polymerase.
Pospiech H, Syväoja JE
TheScientificWorldJournal387-104(2003 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pole gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pole cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa35176	XM_006249562.3 Latest version!	Rattus norvegicus polymerase (DNA) epsilon, catalytic subunit (Pole), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa46173	XM_008769335.2 Latest version!	Rattus norvegicus polymerase (DNA) epsilon, catalytic subunit (Pole), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa05209	NM_001107152.2 Latest version!	Rattus norvegicus DNA polymerase epsilon, catalytic subunit (Pole), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pole ( XM_006249562.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249624.1 Rattus norvegicus polymerase (DNA) epsilon, catalytic subunit (Pole), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa35176
Clone ID Related Accession (Same CDS sequence)	XM_006249562.3
Accession Version	XM_006249562.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DNA polymerase epsilon catalytic subunit A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249562.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA GGAACAGTGG ACGGAGGCAT CCCGAGCCGG GCGCGGACAG CGAAGGCAGC 
CGGGATGATG GTCCCTCCTC TTCAGTCTCA GCACTCAAGC GTCTGGAACG GAGCCAGTGG 
ACGGATAAGA TGGACTTACG GTTTGGTTTT GAAAGGTTGA AAGAGCCTGG AGAAAGGACT 
GGCTGGCTTA TCAACATGCA CCCTACAGAG ATTTTAGATG AAGACAAACG CTTAGTCAGT 
GCAGTGGATT ACTACTTCAT TCAAGATGAT GGAAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC AGCTAGAAAG GGTTGTGATC GAGAAGTTTC ATCTTTTCTA 
TCCAAGAAGT TTCAGGGTAA AATTGCAAAG TTAGAGAATG TGCCCAAAGA AGACCTGGAC 
TTGCCAAACC ACTTGGTGGG CTTGAAGCGG AGTTACATCA AGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG AAAGGAGATC TCTCCTGCTG TGAAGAAGAA CCGAGAGCAG 
GACCATGCTA GTGACGAGTA CACAACAATG CTCTCCAGTA TTCTGCAAGG AGGCAGTCTA 
ATTACTGATG AGGAGGAAAC CTCTAAGAAG ATAGCTGACC AATTGGACAA CATAGTGGAC 
ATGCGGGAGT ATGATGTTCC CTACCACATT CGCCTCTCCA TTGACCTCAG AATCCATGTG 
GCCCACTGGT ACAATGTTAG ATTTCGAGGA AATGCTTTTC CTGTGGAAAT TGCCCGACGA 
GATGATCTCG TCGAACGACC TGATCCTGTG GTTTTGGCAT TTGACATTGA GACAACCAAA 
CTACCTCTCA AATTCCCTGA TGCTGAGACA GATCAGATTA TGATGATCTC CTATATGATT 
GATGGCCAGG GCTACCTCAT CACTAACAGG GAGATTGTTT CAGAAGACAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGG CCTTTCTGTG TCTTCAATGA ACCTGATGAG 
GTCCATCTGA TCCAGAGATG GTTTGAGCAT ATCCAGGAGA CCAAACCTAC CATTATGGTC 
ACCTACAATG GGGATTTTTT TGACTGGCCG TTTGTGGAGG CCAGGGCAGC AATTCATGGC 
CTAAGCATGT ACCAGGAGAT AGGCTTCCAG AAGGATAGCC AGGGGGAATA TAAGGCACCA 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCAGC TGCCAAAGCC AAACTTGGCT ATGACCCTGT GGAGCTGGAC 
CCTGAGGACA TGTGCCGTAT GGCCACTGAA CAGCCCCAGA CTCTGGCCAC TTACTCAGTG 
TCAGATGCTG TTGCTACTTA TTACCTGTAC ATGAAATATG TGCACCCCTT CATATTCGCC 
CTCTGCACCA TTATTCCCAT GGAACCTGAT GAGGTGCTGC GGAAGGGCTC TGGAACATTG 
TGCGAAGCCT TGCTGATGGT GCAAGCCTTC CATGCCAACA TTATCTTTCC CAATAAGCAA 
GAGCAGGAGT TCAACAAGCT GACAGATGAT GGCCACGTGC TAGATGCTGA GACCTACGTT 
GGGGGCCATG TGGAGGCCCT AGAGTCTGGT GTCTTCCGAA GTGATATCCC CTGCCGGTTT 
AGGATGAATC CTGCAGCCTT TGATTTCCTG CTGCAGCGAG TTGAGAAGAC TATGCGCCAT 
GCCATTGAAG AAGAAGAGAA GGTGCCTGTG GAACAAGCCA CCAACTTCCA AGAGGTGTGT 
GAGCAGATTA AGACCAAACT CACCTCCCTA AAAGATGTTC CTAACAGAAT TGAATGTCCT 
CTAATCTACC ATCTAGATGT GGGGGCCATG TATCCTAACA TAATCCTCAC CAACCGCCTG 
CAGCCTTCTG CCATAGTGGA TGAGGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAACTTGTC AGAGGAAAAT GGCCTGGCAA TGGAGGGGAG AATTCATGCC AGCCAGTCGT 
AGTGAGTACC ATCGAATCCA GCATCAGCTA GAGTCGGAGA AGTTCCCCCC TTTGTTCCCA 
GAGGGGCCAG CAAGGGCCTT TCATGAGCTG TCCCGTGAAG AACAGGCTAA ATACGAGAAA 
CGGAGGCTGG CAGATTATTG CCGGAAAGCC TATAAGAAGA TCCATATGAC CAAGGTAGAA 
GAGCGCCTGA CTACCATCTG CCAGCGGGAA AACTCATTTT ATGTGGACAC AGTGCGGGCC 
TTCAGAGACA GGCGCTATGA ATTCAAAGGA CTGCACAAGG TGTGGAAGAA GAAGCTCTCG 
GCGGCTGTAG AGGTGGGCGA TGCATCAGAG GTGAAGCGCT GCAAGAACAT GGAGATCCTT 
TATGATTCGC TGCAGCTGGC TCACAAATGC ATCCTGAACT CATTCTACGG CTATGTTATG 
CGCAAAGGAG CTCGCTGGTA CTCCATGGAG ATGGCTGGTA TCGTCTGCTT TACAGGAGCC 
AACATCATCA CCCAGGCAAG AGAGCTGATT GAGCAGATCG GGAGGCCTTT AGAACTGGAC 
ACGGACGGAA TATGGTGCGT TCTACCCAAT AGCTTTCCTG AAAATTTTGT CATCAAGACA 
ACCAATGTGA AAAAACCCAA ACTGACCATC TCCTATCCTG GTGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTTACCAA CCACCAGTAC CAGGAACTAA CAGAGCCTTC GTCTCTCACC 
TATGTCACCC GCTCTGAGAA CAGCATCTTT TTTGAAGTTG ATGGACCATA CCTTGCTATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAGCTGAAGA AAAGATATGC TGTATTCAAT 
GAAGATGGCT CCTTGGCTGA ACTGAAAGGT TTTGAGGTGA AACGCCGAGG GGAGTTGCAG 
TTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTCC TCAAGGGCAG CACACTAGAG 
GAGGTGTATG CCTCTGTGGC CAAAGTGGCT GACTACTGGC TAGATGTGCT CTATAGCAAG 
GCTGCTAATA TGCCCGATTC TGAATTATTC GAACTGATTT CTGAGAACCG CTCCATGTCA 
CGGAAGCTAG AAGATTATGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCTGAGTTCC TGGGAGACCA GATGGTCAAA GATGCTGGAC TGAGCTGCCG CTACATTATC 
TCCCGAAAGC CAGAGGGATC TCCTGTCACT GAGAGGGCCA TTCCACTTGC CATTTTCCAA 
GCAGAGCCTA CAGTGAGGAA ACATTTTCTC CGGAAATGGT TAAAGAGTTC ATCACTTCAA 
GACTTTGATA TTCGGACAAT TCTGGACTGG GACTACTACA TAGAGCGGCT GGGGAGTGCC 
ATCCAGAAAA TCATCACCAT CCCTGCAGCT CTACAGCAGG TGAAGAACCC AGTTCCACGT 
GTCAAACATC CAGACTGGCT GCACAAAAAG CTACTAGAGA AGAATGATAT CTACAAACAG 
AAGAAGATCA GTGAGCTCTT TGTGCTTGAA GGAAAGAGAC AGATTGTGAT GGCCCAGGCT 
TCAGAAAACA GTCTGAGTCT CTGCACTCCA GACATGGAGG ACTTTGGACT CACAAAGCCA 
TATCACTCTA CAGTTCCAGT TGCTACTAAG AGGAAGCGAG TCTGGGAGAG CCAAAAGGAG 
TCTCAGGATA TTACACTAAC TGTGCCCTGG CAAGAGGTCT TAGGGCAGCC TCCCTCCCTT 
GGAACCACAC AGGAAGAGTG GTTGGTCTGG CTCCAGTTCC ACAAGAAAAA GTGGCAGCTG 
CAGGCTCAAC AGCGCCTAGC CCGCAGGAAG AAACAGCGCT TAGAGTCAGC AGAAGATATG 
CCAAGGCTTG GGGCTATCCG AGAGGGGCCT GCCACAGGAC TGGGGAGCTT TCTGCGACGG 
ACCGCCCGCA GCATCATGGA CCTCCCCTGG CAGATCATAC AGATCAGTGA GACCAGACAG 
GCTGGTCTGT TCCGGCTGTG GGCTATCATT GGCAGCGACT TGCACTGCAT CAAGCTGAGT 
ATCCCTCGAG TATTCTATGT AAACCAGCGG GTAGCCAAAG CAGAGGATGG GCCTTCGTAT 
CGAAAGGTGA ATCGGGCTCT TCCTCGTTCC AACATCGTCT ACAATCTCTA TGAGTACTCA 
GTACCAGAGG ACATGTACCA AGAACACATC AACGAGATCA ACACTGAGTT GTCAGCACCA 
GACATTGAGG GCGTGTATGA GACTCAGGTC CCATTGTTAT TCCGGGCCCT GGTGCAGCTG 
GGCTGTGTGT GTGTGGTCAA CAAACAGCTG GCAAGGCAAC TTTCAGGCTG GGAAGCTGAA 
ACCTTTGCCC TCGAGCACCT TGAAATGCGT TCTCTGGCCC AGTTCAGCTA CTTGGAACCA 
GGGAGTATCC GCCACATCTA CCTGTACCAT CACACACAGG GCCACAAGGC ACTCTTTGGG 
GTCTTTATCC CCTCACAGCG AAGAGCATCT GTGTTTGTGT TGGATACTGT GCGCAGCAAC 
CAAATGCCAG GGCTCAGTGC CCTATACTCA GCAGAACACA GCCTGCTGCT GGACAAGGTG 
GGCCCCAACC TCCTGCCTCC CCCAAAACAC ACCTTTGAAG TTCGTGCTGA AACCAACCTG 
AAGACCATCT GCAGAGCCAT CCAACGCTTC CTGCTTGCCT ACAAGGAAGA GCGCCGGGGG 
CCCACGCTCA TCGCTATCCA GTCTAGCTGG GAACTGTGTA GGCTGACCAG TGAGATTCCA 
GTCTTAGAGG AGTTCCCACT AGTGCCTGTC CGAGTGGCTG ACAAGATCAG CTATGCAGTC 
CTGGACTGGC AGCGCCACGG AGCTCGCCGA ATGATCCGGC ACTACCTCAA TTTAGACATT 
TGCCTTTCAC AGGCCTTTGA GATGAGCAGG TACTTCCACA TCCCCGTTGG AAACCTGCCA 
GAAGACATCT CCACCTTTGG CTCTGACCTC TTTTTTGCAC GCCATCTCCA GCGCCATAAC 
CACCTACTTT GGTTGTCCCC TACCTCTCGG CCTGACCTGG GTGGGAAGGA AGCTGATGAC 
AACCGCCTTG TCATGGAGTT TGATGACCGA GCCACTGTGG AGATCAATAA TTCCGGCTGT 
TACTCTACTG TGTGCGTGGA GCTGGACATT CAAAACCTGG CAGTCAACAC CATCCTCCAG 
TCCCACCATG TCAATGACAT GGAGGGGGCT GGCAGCATGG GCATCAGCTT CGATGTGATC 
CAGCAGGCCT CCCTAGAGGA CATGGTTACA GGCAATCAAG CTGCCAGTGC CCTGGCCAGC 
TACGATGAGA CAGCCCTCTG CTCCAGTACG TTCAGGATCC TGAAGAGCAT GGTGGTTGGC 
TGGGTAAAGG AAATCACACA GTACCACAAC ATCTATGCTG ACAACCAGGT GATGCACTTC 
TACCGCTGGC TCCAGTCGCC GTGCTCTCTG CTCCACGACC CAGCCCTTCA CCGGACACTG 
CACAATATGA TGAAGAAGCT CTTCCTGCAG CTCATTGCTG AGTTCAAGCG CCTGGGGTCA 
TCGGTCATCT ATGCCAACTT CAATCGCATC ATCCTCTGTA CAAAGAAGCG CCGGATAGAG 
GATGCCCTTG CCTATGTGGA ATATATCACC AACAGCATCC ATTCTAAAGA GATCTTTCAT 
TCCCTGACCA TCTCTTTCTC TCGATGCTGG GAATTCCTTC TCTGGATGGA TCCATCCAAC 
TATGGTGGAA TCAAAGGAAA AGTCCCATCC AGTATTCACT GTGGACAAGT AAGGGAGCAT 
ACATCGTGGC TGTGTACCAA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249624.1
CDS	76..5922
Translation	MVLRNSGRRHPEPGADSEGSRDDGPSSSVSALKRLERSQWTDKMDLRFGFERLKEPGERTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYKPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLDLPNHLVGLKRSYIKLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSLITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEIARRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEHIQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAIVDEATCAACDFNKPGATCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHMTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNVKKPKLTISYPGAMLNIMVKEGFTNHQYQELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYASVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDIYKQKKISELFVLEGKRQIVMAQASENSLSLCTPDMEDFGLTKPYHSTVPVATKRKRVWESQKESQDITLTVPWQEVLGQPPSLGTTQEEWLVWLQFHKKKWQLQAQQRLARRKKQRLESAEDMPRLGAIREGPATGLGSFLRRTARSIMDLPWQIIQISETRQAGLFRLWAIIGSDLHCIKLSIPRVFYVNQRVAKAEDGPSYRKVNRALPRSNIVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLARQLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHTQGHKALFGVFIPSQRRASVFVLDTVRSNQMPGLSALYSAEHSLLLDKVGPNLLPPPKHTFEVRAETNLKTICRAIQRFLLAYKEERRGPTLIAIQSSWELCRLTSEIPVLEEFPLVPVRVADKISYAVLDWQRHGARRMIRHYLNLDICLSQAFEMSRYFHIPVGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDRATVEINNSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGAGSMGISFDVIQQASLEDMVTGNQAASALASYDETALCSSTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLQSPCSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRIEDALAYVEYITNSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGKVPSSIHCGQVREHTSWLCTKA

Target ORF information:

RefSeq Version	XM_006249562.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polymerase (DNA) epsilon, catalytic subunit (Pole), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249562.3

ORF Insert Sequence:

ATGGTGCTGA GGAACAGTGG ACGGAGGCAT CCCGAGCCGG GCGCGGACAG CGAAGGCAGC 
CGGGATGATG GTCCCTCCTC TTCAGTCTCA GCACTCAAGC GTCTGGAACG GAGCCAGTGG 
ACGGATAAGA TGGACTTACG GTTTGGTTTT GAAAGGTTGA AAGAGCCTGG AGAAAGGACT 
GGCTGGCTTA TCAACATGCA CCCTACAGAG ATTTTAGATG AAGACAAACG CTTAGTCAGT 
GCAGTGGATT ACTACTTCAT TCAAGATGAT GGAAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC AGCTAGAAAG GGTTGTGATC GAGAAGTTTC ATCTTTTCTA 
TCCAAGAAGT TTCAGGGTAA AATTGCAAAG TTAGAGAATG TGCCCAAAGA AGACCTGGAC 
TTGCCAAACC ACTTGGTGGG CTTGAAGCGG AGTTACATCA AGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG AAAGGAGATC TCTCCTGCTG TGAAGAAGAA CCGAGAGCAG 
GACCATGCTA GTGACGAGTA CACAACAATG CTCTCCAGTA TTCTGCAAGG AGGCAGTCTA 
ATTACTGATG AGGAGGAAAC CTCTAAGAAG ATAGCTGACC AATTGGACAA CATAGTGGAC 
ATGCGGGAGT ATGATGTTCC CTACCACATT CGCCTCTCCA TTGACCTCAG AATCCATGTG 
GCCCACTGGT ACAATGTTAG ATTTCGAGGA AATGCTTTTC CTGTGGAAAT TGCCCGACGA 
GATGATCTCG TCGAACGACC TGATCCTGTG GTTTTGGCAT TTGACATTGA GACAACCAAA 
CTACCTCTCA AATTCCCTGA TGCTGAGACA GATCAGATTA TGATGATCTC CTATATGATT 
GATGGCCAGG GCTACCTCAT CACTAACAGG GAGATTGTTT CAGAAGACAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGG CCTTTCTGTG TCTTCAATGA ACCTGATGAG 
GTCCATCTGA TCCAGAGATG GTTTGAGCAT ATCCAGGAGA CCAAACCTAC CATTATGGTC 
ACCTACAATG GGGATTTTTT TGACTGGCCG TTTGTGGAGG CCAGGGCAGC AATTCATGGC 
CTAAGCATGT ACCAGGAGAT AGGCTTCCAG AAGGATAGCC AGGGGGAATA TAAGGCACCA 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCAGC TGCCAAAGCC AAACTTGGCT ATGACCCTGT GGAGCTGGAC 
CCTGAGGACA TGTGCCGTAT GGCCACTGAA CAGCCCCAGA CTCTGGCCAC TTACTCAGTG 
TCAGATGCTG TTGCTACTTA TTACCTGTAC ATGAAATATG TGCACCCCTT CATATTCGCC 
CTCTGCACCA TTATTCCCAT GGAACCTGAT GAGGTGCTGC GGAAGGGCTC TGGAACATTG 
TGCGAAGCCT TGCTGATGGT GCAAGCCTTC CATGCCAACA TTATCTTTCC CAATAAGCAA 
GAGCAGGAGT TCAACAAGCT GACAGATGAT GGCCACGTGC TAGATGCTGA GACCTACGTT 
GGGGGCCATG TGGAGGCCCT AGAGTCTGGT GTCTTCCGAA GTGATATCCC CTGCCGGTTT 
AGGATGAATC CTGCAGCCTT TGATTTCCTG CTGCAGCGAG TTGAGAAGAC TATGCGCCAT 
GCCATTGAAG AAGAAGAGAA GGTGCCTGTG GAACAAGCCA CCAACTTCCA AGAGGTGTGT 
GAGCAGATTA AGACCAAACT CACCTCCCTA AAAGATGTTC CTAACAGAAT TGAATGTCCT 
CTAATCTACC ATCTAGATGT GGGGGCCATG TATCCTAACA TAATCCTCAC CAACCGCCTG 
CAGCCTTCTG CCATAGTGGA TGAGGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAACTTGTC AGAGGAAAAT GGCCTGGCAA TGGAGGGGAG AATTCATGCC AGCCAGTCGT 
AGTGAGTACC ATCGAATCCA GCATCAGCTA GAGTCGGAGA AGTTCCCCCC TTTGTTCCCA 
GAGGGGCCAG CAAGGGCCTT TCATGAGCTG TCCCGTGAAG AACAGGCTAA ATACGAGAAA 
CGGAGGCTGG CAGATTATTG CCGGAAAGCC TATAAGAAGA TCCATATGAC CAAGGTAGAA 
GAGCGCCTGA CTACCATCTG CCAGCGGGAA AACTCATTTT ATGTGGACAC AGTGCGGGCC 
TTCAGAGACA GGCGCTATGA ATTCAAAGGA CTGCACAAGG TGTGGAAGAA GAAGCTCTCG 
GCGGCTGTAG AGGTGGGCGA TGCATCAGAG GTGAAGCGCT GCAAGAACAT GGAGATCCTT 
TATGATTCGC TGCAGCTGGC TCACAAATGC ATCCTGAACT CATTCTACGG CTATGTTATG 
CGCAAAGGAG CTCGCTGGTA CTCCATGGAG ATGGCTGGTA TCGTCTGCTT TACAGGAGCC 
AACATCATCA CCCAGGCAAG AGAGCTGATT GAGCAGATCG GGAGGCCTTT AGAACTGGAC 
ACGGACGGAA TATGGTGCGT TCTACCCAAT AGCTTTCCTG AAAATTTTGT CATCAAGACA 
ACCAATGTGA AAAAACCCAA ACTGACCATC TCCTATCCTG GTGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTTACCAA CCACCAGTAC CAGGAACTAA CAGAGCCTTC GTCTCTCACC 
TATGTCACCC GCTCTGAGAA CAGCATCTTT TTTGAAGTTG ATGGACCATA CCTTGCTATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAGCTGAAGA AAAGATATGC TGTATTCAAT 
GAAGATGGCT CCTTGGCTGA ACTGAAAGGT TTTGAGGTGA AACGCCGAGG GGAGTTGCAG 
TTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTCC TCAAGGGCAG CACACTAGAG 
GAGGTGTATG CCTCTGTGGC CAAAGTGGCT GACTACTGGC TAGATGTGCT CTATAGCAAG 
GCTGCTAATA TGCCCGATTC TGAATTATTC GAACTGATTT CTGAGAACCG CTCCATGTCA 
CGGAAGCTAG AAGATTATGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCTGAGTTCC TGGGAGACCA GATGGTCAAA GATGCTGGAC TGAGCTGCCG CTACATTATC 
TCCCGAAAGC CAGAGGGATC TCCTGTCACT GAGAGGGCCA TTCCACTTGC CATTTTCCAA 
GCAGAGCCTA CAGTGAGGAA ACATTTTCTC CGGAAATGGT TAAAGAGTTC ATCACTTCAA 
GACTTTGATA TTCGGACAAT TCTGGACTGG GACTACTACA TAGAGCGGCT GGGGAGTGCC 
ATCCAGAAAA TCATCACCAT CCCTGCAGCT CTACAGCAGG TGAAGAACCC AGTTCCACGT 
GTCAAACATC CAGACTGGCT GCACAAAAAG CTACTAGAGA AGAATGATAT CTACAAACAG 
AAGAAGATCA GTGAGCTCTT TGTGCTTGAA GGAAAGAGAC AGATTGTGAT GGCCCAGGCT 
TCAGAAAACA GTCTGAGTCT CTGCACTCCA GACATGGAGG ACTTTGGACT CACAAAGCCA 
TATCACTCTA CAGTTCCAGT TGCTACTAAG AGGAAGCGAG TCTGGGAGAG CCAAAAGGAG 
TCTCAGGATA TTACACTAAC TGTGCCCTGG CAAGAGGTCT TAGGGCAGCC TCCCTCCCTT 
GGAACCACAC AGGAAGAGTG GTTGGTCTGG CTCCAGTTCC ACAAGAAAAA GTGGCAGCTG 
CAGGCTCAAC AGCGCCTAGC CCGCAGGAAG AAACAGCGCT TAGAGTCAGC AGAAGATATG 
CCAAGGCTTG GGGCTATCCG AGAGGGGCCT GCCACAGGAC TGGGGAGCTT TCTGCGACGG 
ACCGCCCGCA GCATCATGGA CCTCCCCTGG CAGATCATAC AGATCAGTGA GACCAGACAG 
GCTGGTCTGT TCCGGCTGTG GGCTATCATT GGCAGCGACT TGCACTGCAT CAAGCTGAGT 
ATCCCTCGAG TATTCTATGT AAACCAGCGG GTAGCCAAAG CAGAGGATGG GCCTTCGTAT 
CGAAAGGTGA ATCGGGCTCT TCCTCGTTCC AACATCGTCT ACAATCTCTA TGAGTACTCA 
GTACCAGAGG ACATGTACCA AGAACACATC AACGAGATCA ACACTGAGTT GTCAGCACCA 
GACATTGAGG GCGTGTATGA GACTCAGGTC CCATTGTTAT TCCGGGCCCT GGTGCAGCTG 
GGCTGTGTGT GTGTGGTCAA CAAACAGCTG GCAAGGCAAC TTTCAGGCTG GGAAGCTGAA 
ACCTTTGCCC TCGAGCACCT TGAAATGCGT TCTCTGGCCC AGTTCAGCTA CTTGGAACCA 
GGGAGTATCC GCCACATCTA CCTGTACCAT CACACACAGG GCCACAAGGC ACTCTTTGGG 
GTCTTTATCC CCTCACAGCG AAGAGCATCT GTGTTTGTGT TGGATACTGT GCGCAGCAAC 
CAAATGCCAG GGCTCAGTGC CCTATACTCA GCAGAACACA GCCTGCTGCT GGACAAGGTG 
GGCCCCAACC TCCTGCCTCC CCCAAAACAC ACCTTTGAAG TTCGTGCTGA AACCAACCTG 
AAGACCATCT GCAGAGCCAT CCAACGCTTC CTGCTTGCCT ACAAGGAAGA GCGCCGGGGG 
CCCACGCTCA TCGCTATCCA GTCTAGCTGG GAACTGTGTA GGCTGACCAG TGAGATTCCA 
GTCTTAGAGG AGTTCCCACT AGTGCCTGTC CGAGTGGCTG ACAAGATCAG CTATGCAGTC 
CTGGACTGGC AGCGCCACGG AGCTCGCCGA ATGATCCGGC ACTACCTCAA TTTAGACATT 
TGCCTTTCAC AGGCCTTTGA GATGAGCAGG TACTTCCACA TCCCCGTTGG AAACCTGCCA 
GAAGACATCT CCACCTTTGG CTCTGACCTC TTTTTTGCAC GCCATCTCCA GCGCCATAAC 
CACCTACTTT GGTTGTCCCC TACCTCTCGG CCTGACCTGG GTGGGAAGGA AGCTGATGAC 
AACCGCCTTG TCATGGAGTT TGATGACCGA GCCACTGTGG AGATCAATAA TTCCGGCTGT 
TACTCTACTG TGTGCGTGGA GCTGGACATT CAAAACCTGG CAGTCAACAC CATCCTCCAG 
TCCCACCATG TCAATGACAT GGAGGGGGCT GGCAGCATGG GCATCAGCTT CGATGTGATC 
CAGCAGGCCT CCCTAGAGGA CATGGTTACA GGCAATCAAG CTGCCAGTGC CCTGGCCAGC 
TACGATGAGA CAGCCCTCTG CTCCAGTACG TTCAGGATCC TGAAGAGCAT GGTGGTTGGC 
TGGGTAAAGG AAATCACACA GTACCACAAC ATCTATGCTG ACAACCAGGT GATGCACTTC 
TACCGCTGGC TCCAGTCGCC GTGCTCTCTG CTCCACGACC CAGCCCTTCA CCGGACACTG 
CACAATATGA TGAAGAAGCT CTTCCTGCAG CTCATTGCTG AGTTCAAGCG CCTGGGGTCA 
TCGGTCATCT ATGCCAACTT CAATCGCATC ATCCTCTGTA CAAAGAAGCG CCGGATAGAG 
GATGCCCTTG CCTATGTGGA ATATATCACC AACAGCATCC ATTCTAAAGA GATCTTTCAT 
TCCCTGACCA TCTCTTTCTC TCGATGCTGG GAATTCCTTC TCTGGATGGA TCCATCCAAC 
TATGGTGGAA TCAAAGGAAA AGTCCCATCC AGTATTCACT GTGGACAAGT AAGGGAGCAT 
ACATCGTGGC TGTGTACCAA AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pole ( XM_008769335.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767557.1 Rattus norvegicus polymerase (DNA) epsilon, catalytic subunit (Pole), transcript variant X1, mRNA.

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CloneID	ORa46173
Clone ID Related Accession (Same CDS sequence)	XM_008769335.2
Accession Version	XM_008769335.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	DNA polymerase epsilon catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769335.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGA GGAACAGTGG ACGGAGGCAT CCCGAGCCGG GCGCGGACAG CGAAGGCAGC 
CGGGATGATG GTCCCTCCTC TTCAGTCTCA GCACTCAAGC GTCTGGAACG GAGCCAGTGG 
ACGGATAAGA TGGACTTACG GTTTGGTTTT GAAAGGTTGA AAGAGCCTGG AGAAAGGACT 
GGCTGGCTTA TCAACATGCA CCCTACAGAG ATTTTAGATG AAGACAAACG CTTAGTCAGT 
GCAGTGGATT ACTACTTCAT TCAAGATGAT GGAAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC AGCTAGAAAG GGTTGTGATC GAGAAGTTTC ATCTTTTCTA 
TCCAAGAAGT TTCAGGGTAA AATTGCAAAG TTAGAGAATG TGCCCAAAGA AGACCTGGAC 
TTGCCAAACC ACTTGGTGGG CTTGAAGCGG AGTTACATCA AGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG AAAGGAGATC TCTCCTGCTG TGAAGAAGAA CCGAGAGCAG 
GACCATGCTA GTGACGAGTA CACAACAATG CTCTCCAGTA TTCTGCAAGG AGGCAGTCTA 
ATTACTGATG AGGAGGAAAC CTCTAAGAAG ATAGCTGACC AATTGGACAA CATAGTGGAC 
ATGCGGGAGT ATGATGTTCC CTACCACATT CGCCTCTCCA TTGACCTCAG AATCCATGTG 
GCCCACTGGT ACAATGTTAG ATTTCGAGGA AATGCTTTTC CTGTGGAAAT TGCCCGACGA 
GATGATCTCG TCGAACGACC TGATCCTGTG GTTTTGGCAT TTGACATTGA GACAACCAAA 
CTACCTCTCA AATTCCCTGA TGCTGAGACA GATCAGATTA TGATGATCTC CTATATGATT 
GATGGCCAGG GCTACCTCAT CACTAACAGG GAGATTGTTT CAGAAGACAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGG CCTTTCTGTG TCTTCAATGA ACCTGATGAG 
GTCCATCTGA TCCAGAGATG GTTTGAGCAT ATCCAGGAGA CCAAACCTAC CATTATGGTC 
ACCTACAATG GGGATTTTTT TGACTGGCCG TTTGTGGAGG CCAGGGCAGC AATTCATGGC 
CTAAGCATGT ACCAGGAGAT AGGCTTCCAG AAGGATAGCC AGGGGGAATA TAAGGCACCA 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCAGC TGCCAAAGCC AAACTTGGCT ATGACCCTGT GGAGCTGGAC 
CCTGAGGACA TGTGCCGTAT GGCCACTGAA CAGCCCCAGA CTCTGGCCAC TTACTCAGTG 
TCAGATGCTG TTGCTACTTA TTACCTGTAC ATGAAATATG TGCACCCCTT CATATTCGCC 
CTCTGCACCA TTATTCCCAT GGAACCTGAT GAGGTGCTGC GGAAGGGCTC TGGAACATTG 
TGCGAAGCCT TGCTGATGGT GCAAGCCTTC CATGCCAACA TTATCTTTCC CAATAAGCAA 
GAGCAGGAGT TCAACAAGCT GACAGATGAT GGCCACGTGC TAGATGCTGA GACCTACGTT 
GGGGGCCATG TGGAGGCCCT AGAGTCTGGT GTCTTCCGAA GTGATATCCC CTGCCGGTTT 
AGGATGAATC CTGCAGCCTT TGATTTCCTG CTGCAGCGAG TTGAGAAGAC TATGCGCCAT 
GCCATTGAAG AAGAAGAGAA GGTGCCTGTG GAACAAGCCA CCAACTTCCA AGAGGTGTGT 
GAGCAGATTA AGACCAAACT CACCTCCCTA AAAGATGTTC CTAACAGAAT TGAATGTCCT 
CTAATCTACC ATCTAGATGT GGGGGCCATG TATCCTAACA TAATCCTCAC CAACCGCCTG 
CAGCCTTCTG CCATAGTGGA TGAGGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAACTTGTC AGAGGAAAAT GGCCTGGCAA TGGAGGGGAG AATTCATGCC AGCCAGTCGT 
AGTGAGTACC ATCGAATCCA GCATCAGCTA GAGTCGGAGA AGTTCCCCCC TTTGTTCCCA 
GAGGGGCCAG CAAGGGCCTT TCATGAGCTG TCCCGTGAAG AACAGGCTAA ATACGAGAAA 
CGGAGGCTGG CAGATTATTG CCGGAAAGCC TATAAGAAGA TCCATATGAC CAAGGTAGAA 
GAGCGCCTGA CTACCATCTG CCAGCGGGAA AACTCATTTT ATGTGGACAC AGTGCGGGCC 
TTCAGAGACA GGCGCTATGA ATTCAAAGGA CTGCACAAGG TGTGGAAGAA GAAGCTCTCG 
GCGGCTGTAG AGGTGGGCGA TGCATCAGAG GTGAAGCGCT GCAAGAACAT GGAGATCCTT 
TATGATTCGC TGCAGCTGGC TCACAAATGC ATCCTGAACT CATTCTACGG CTATGTTATG 
CGCAAAGGAG CTCGCTGGTA CTCCATGGAG ATGGCTGGTA TCGTCTGCTT TACAGGAGCC 
AACATCATCA CCCAGGCAAG AGAGCTGATT GAGCAGATCG GGAGGCCTTT AGAACTGGAC 
ACGGACGGAA TATGGTGCGT TCTACCCAAT AGCTTTCCTG AAAATTTTGT CATCAAGACA 
ACCAATGTGA AAAAACCCAA ACTGACCATC TCCTATCCTG GTGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTTACCAA CCACCAGTAC CAGGAACTAA CAGAGCCTTC GTCTCTCACC 
TATGTCACCC GCTCTGAGAA CAGCATCTTT TTTGAAGTTG ATGGACCATA CCTTGCTATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAGCTGAAGA AAAGATATGC TGTATTCAAT 
GAAGATGGCT CCTTGGCTGA ACTGAAAGGT TTTGAGGTGA AACGCCGAGG GGAGTTGCAG 
TTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTCC TCAAGGGCAG CACACTAGAG 
GAGGTGTATG CCTCTGTGGC CAAAGTGGCT GACTACTGGC TAGATGTGCT CTATAGCAAG 
GCTGCTAATA TGCCCGATTC TGAATTATTC GAACTGATTT CTGAGAACCG CTCCATGTCA 
CGGAAGCTAG AAGATTATGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCTGAGTTCC TGGGAGACCA GATGGTCAAA GATGCTGGAC TGAGCTGCCG CTACATTATC 
TCCCGAAAGC CAGAGGGATC TCCTGTCACT GAGAGGGCCA TTCCACTTGC CATTTTCCAA 
GCAGAGCCTA CAGTGAGGAA ACATTTTCTC CGGAAATGGT TAAAGAGTTC ATCACTTCAA 
GACTTTGATA TTCGGACAAT TCTGGACTGG GACTACTACA TAGAGCGGCT GGGGAGTGCC 
ATCCAGAAAA TCATCACCAT CCCTGCAGCT CTACAGCAGG TGAAGAACCC AGTTCCACGT 
GTCAAACATC CAGACTGGCT GCACAAAAAG CTACTAGAGA AGAATGATAT CTACAAACAG 
AAGAAGATCA GTGAGCTCTT TGTGCTTGAA GGAAAGAGAC AGATTGTGAT GGCCCAGGCT 
TCAGAAAACA GTCTGAGTCT CTGCACTCCA GACATGGAGG ACTTTGGACT CACAAAGCCA 
TATCACTCTA CAGTTCCAGT TGCTACTAAG AGGAAGCGAG TCTGGGAGAG CCAAAAGGAG 
TCTCAGGATA TTACACTAAC TGTGCCCTGG CAAGAGGTCT TAGGGCAGCC TCCCTCCCTT 
GGAACCACAC AGGAAGAGTG GTTGGTCTGG CTCCAGTTCC ACAAGAAAAA GTGGCAGCTG 
CAGGCTCAAC AGCGCCTAGC CCGCAGGAAG AAACAGCGCT TAGAGTCAGC AGAAGATATG 
CCAAGGCTTG GGGCTATCCG AGAGGGGCCT GCCACAGGAC TGGGGAGCTT TCTGCGACGG 
ACCGCCCGCA GCATCATGGA CCTCCCCTGG CAGATCATAC AGATCAGTGA GACCAGACAG 
GCTGGTCTGT TCCGGCTGTG GGCTATCATT GGCAGCGACT TGCACTGCAT CAAGCTGAGT 
ATCCCTCGAG TATTCTATGT AAACCAGCGG GTAGCCAAAG CAGAGGATGG GCCTTCGTAT 
CGAAAGGTGA ATCGGGCTCT TCCTCGTTCC AACATCGTCT ACAATCTCTA TGAGTACTCA 
GTACCAGAGG ACATGTACCA AGAACACATC AACGAGATCA ACACTGAGTT GTCAGCACCA 
GACATTGAGG GCGTGTATGA GACTCAGGTC CCATTGTTAT TCCGGGCCCT GGTGCAGCTG 
GGCTGTGTGT GTGTGGTCAA CAAACAGCTG GCAAGGCAAC TTTCAGGCTG GGAAGCTGAA 
ACCTTTGCCC TCGAGCACCT TGAAATGCGT TCTCTGGCCC AGTTCAGCTA CTTGGAACCA 
GGGAGTATCC GCCACATCTA CCTGTACCAT CACACACAGG GCCACAAGGC ACTCTTTGGG 
GTCTTTATCC CCTCACAGCG AAGAGCATCT GTGTTTGTGT TGGATACTGT GCGCAGCAAC 
CAAATGCCAG GGCTCAGTGC CCTATACTCA GCAGAACACA GCCTGCTGCT GGACAAGGTG 
GGCCCCAACC TCCTGCCTCC CCCAAAACAC ACCTTTGAAG TTCGTGCTGA AACCAACCTG 
AAGACCATCT GCAGAGCCAT CCAACGCTTC CTGCTTGCCT ACAAGGAAGA GCGCCGGGGG 
CCCACGCTCA TCGCTATCCA GTCTAGCTGG GAACTGTGTA GGCTGACCAG TGAGATTCCA 
GTCTTAGAGG AGTTCCCACT AGTGCCTGTC CGAGTGGCTG ACAAGATCAG CTATGCAGTC 
CTGGACTGGC AGCGCCACGG AGCTCGCCGA ATGATCCGGC ACTACCTCAA TTTAGACATT 
TGCCTTTCAC AGGCCTTTGA GATGAGCAGG TACTTCCACA TCCCCGTTGG AAACCTGCCA 
GAAGACATCT CCACCTTTGG CTCTGACCTC TTTTTTGCAC GCCATCTCCA GCGCCATAAC 
CACCTACTTT GGTTGTCCCC TACCTCTCGG CCTGACCTGG GTGGGAAGGA AGCTGATGAC 
AACCGCCTTG TCATGGAGTT TGATGACCGA GCCACTGTGG AGATCAATAA TTCCGGCTGT 
TACTCTACTG TGTGCGTGGA GCTGGACATT CAAAACCTGG CAGTCAACAC CATCCTCCAG 
TCCCACCATG TCAATGACAT GGAGGGGGCT GGCAGCATGG GCATCAGCTT CGATGTGATC 
CAGCAGGCCT CCCTAGAGGA CATGGTTACA GGCAATCAAG CTGCCAGTGC CCTGGCCAGC 
TACGATGAGA CAGCCCTCTG CTCCAGTACG TTCAGGATCC TGAAGAGCAT GGTGGTTGGC 
TGGGTAAAGG AAATCACACA GTACCACAAC ATCTATGCTG ACAACCAGGT GATGCACTTC 
TACCGCTGGC TCCAGTCGCC GTGCTCTCTG CTCCACGACC CAGCCCTTCA CCGGACACTG 
CACAATATGA TGAAGAAGCT CTTCCTGCAG CTCATTGCTG AGTTCAAGCG CCTGGGGTCA 
TCGGTCATCT ATGCCAACTT CAATCGCATC ATCCTCTGTA CAAAGAAGCG CCGGATAGAG 
GATGCCCTTG CCTATGTGGA ATATATCACC AACAGCATCC ATTCTAAAGA GATCTTTCAT 
TCCCTGACCA TCTCTTTCTC TCGATGCTGG GAATTCCTTC TCTGGATGGA TCCATCCAAC 
TATGGTGGAA TCAAAGGAAA AGTCCCATCC AGTATTCACT GTGGACAAGT AAGGGAGCAA 
GACACCCTGG GAACTGGGCA GCTCAGGCAC ATTGGGTGTT TGGGCAGTAG TTTCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767557.1
CDS	77..5956
Translation	MVLRNSGRRHPEPGADSEGSRDDGPSSSVSALKRLERSQWTDKMDLRFGFERLKEPGERTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYKPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLDLPNHLVGLKRSYIKLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSLITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEIARRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEHIQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAIVDEATCAACDFNKPGATCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHMTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNVKKPKLTISYPGAMLNIMVKEGFTNHQYQELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYASVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDIYKQKKISELFVLEGKRQIVMAQASENSLSLCTPDMEDFGLTKPYHSTVPVATKRKRVWESQKESQDITLTVPWQEVLGQPPSLGTTQEEWLVWLQFHKKKWQLQAQQRLARRKKQRLESAEDMPRLGAIREGPATGLGSFLRRTARSIMDLPWQIIQISETRQAGLFRLWAIIGSDLHCIKLSIPRVFYVNQRVAKAEDGPSYRKVNRALPRSNIVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLARQLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHTQGHKALFGVFIPSQRRASVFVLDTVRSNQMPGLSALYSAEHSLLLDKVGPNLLPPPKHTFEVRAETNLKTICRAIQRFLLAYKEERRGPTLIAIQSSWELCRLTSEIPVLEEFPLVPVRVADKISYAVLDWQRHGARRMIRHYLNLDICLSQAFEMSRYFHIPVGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDRATVEINNSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGAGSMGISFDVIQQASLEDMVTGNQAASALASYDETALCSSTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLQSPCSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRIEDALAYVEYITNSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGKVPSSIHCGQVREQDTLGTGQLRHIGCLGSSFR

Target ORF information:

RefSeq Version	XM_008769335.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polymerase (DNA) epsilon, catalytic subunit (Pole), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769335.2

ORF Insert Sequence:

ATGGTGCTGA GGAACAGTGG ACGGAGGCAT CCCGAGCCGG GCGCGGACAG CGAAGGCAGC 
CGGGATGATG GTCCCTCCTC TTCAGTCTCA GCACTCAAGC GTCTGGAACG GAGCCAGTGG 
ACGGATAAGA TGGACTTACG GTTTGGTTTT GAAAGGTTGA AAGAGCCTGG AGAAAGGACT 
GGCTGGCTTA TCAACATGCA CCCTACAGAG ATTTTAGATG AAGACAAACG CTTAGTCAGT 
GCAGTGGATT ACTACTTCAT TCAAGATGAT GGAAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC AGCTAGAAAG GGTTGTGATC GAGAAGTTTC ATCTTTTCTA 
TCCAAGAAGT TTCAGGGTAA AATTGCAAAG TTAGAGAATG TGCCCAAAGA AGACCTGGAC 
TTGCCAAACC ACTTGGTGGG CTTGAAGCGG AGTTACATCA AGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG AAAGGAGATC TCTCCTGCTG TGAAGAAGAA CCGAGAGCAG 
GACCATGCTA GTGACGAGTA CACAACAATG CTCTCCAGTA TTCTGCAAGG AGGCAGTCTA 
ATTACTGATG AGGAGGAAAC CTCTAAGAAG ATAGCTGACC AATTGGACAA CATAGTGGAC 
ATGCGGGAGT ATGATGTTCC CTACCACATT CGCCTCTCCA TTGACCTCAG AATCCATGTG 
GCCCACTGGT ACAATGTTAG ATTTCGAGGA AATGCTTTTC CTGTGGAAAT TGCCCGACGA 
GATGATCTCG TCGAACGACC TGATCCTGTG GTTTTGGCAT TTGACATTGA GACAACCAAA 
CTACCTCTCA AATTCCCTGA TGCTGAGACA GATCAGATTA TGATGATCTC CTATATGATT 
GATGGCCAGG GCTACCTCAT CACTAACAGG GAGATTGTTT CAGAAGACAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGG CCTTTCTGTG TCTTCAATGA ACCTGATGAG 
GTCCATCTGA TCCAGAGATG GTTTGAGCAT ATCCAGGAGA CCAAACCTAC CATTATGGTC 
ACCTACAATG GGGATTTTTT TGACTGGCCG TTTGTGGAGG CCAGGGCAGC AATTCATGGC 
CTAAGCATGT ACCAGGAGAT AGGCTTCCAG AAGGATAGCC AGGGGGAATA TAAGGCACCA 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCAGC TGCCAAAGCC AAACTTGGCT ATGACCCTGT GGAGCTGGAC 
CCTGAGGACA TGTGCCGTAT GGCCACTGAA CAGCCCCAGA CTCTGGCCAC TTACTCAGTG 
TCAGATGCTG TTGCTACTTA TTACCTGTAC ATGAAATATG TGCACCCCTT CATATTCGCC 
CTCTGCACCA TTATTCCCAT GGAACCTGAT GAGGTGCTGC GGAAGGGCTC TGGAACATTG 
TGCGAAGCCT TGCTGATGGT GCAAGCCTTC CATGCCAACA TTATCTTTCC CAATAAGCAA 
GAGCAGGAGT TCAACAAGCT GACAGATGAT GGCCACGTGC TAGATGCTGA GACCTACGTT 
GGGGGCCATG TGGAGGCCCT AGAGTCTGGT GTCTTCCGAA GTGATATCCC CTGCCGGTTT 
AGGATGAATC CTGCAGCCTT TGATTTCCTG CTGCAGCGAG TTGAGAAGAC TATGCGCCAT 
GCCATTGAAG AAGAAGAGAA GGTGCCTGTG GAACAAGCCA CCAACTTCCA AGAGGTGTGT 
GAGCAGATTA AGACCAAACT CACCTCCCTA AAAGATGTTC CTAACAGAAT TGAATGTCCT 
CTAATCTACC ATCTAGATGT GGGGGCCATG TATCCTAACA TAATCCTCAC CAACCGCCTG 
CAGCCTTCTG CCATAGTGGA TGAGGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAACTTGTC AGAGGAAAAT GGCCTGGCAA TGGAGGGGAG AATTCATGCC AGCCAGTCGT 
AGTGAGTACC ATCGAATCCA GCATCAGCTA GAGTCGGAGA AGTTCCCCCC TTTGTTCCCA 
GAGGGGCCAG CAAGGGCCTT TCATGAGCTG TCCCGTGAAG AACAGGCTAA ATACGAGAAA 
CGGAGGCTGG CAGATTATTG CCGGAAAGCC TATAAGAAGA TCCATATGAC CAAGGTAGAA 
GAGCGCCTGA CTACCATCTG CCAGCGGGAA AACTCATTTT ATGTGGACAC AGTGCGGGCC 
TTCAGAGACA GGCGCTATGA ATTCAAAGGA CTGCACAAGG TGTGGAAGAA GAAGCTCTCG 
GCGGCTGTAG AGGTGGGCGA TGCATCAGAG GTGAAGCGCT GCAAGAACAT GGAGATCCTT 
TATGATTCGC TGCAGCTGGC TCACAAATGC ATCCTGAACT CATTCTACGG CTATGTTATG 
CGCAAAGGAG CTCGCTGGTA CTCCATGGAG ATGGCTGGTA TCGTCTGCTT TACAGGAGCC 
AACATCATCA CCCAGGCAAG AGAGCTGATT GAGCAGATCG GGAGGCCTTT AGAACTGGAC 
ACGGACGGAA TATGGTGCGT TCTACCCAAT AGCTTTCCTG AAAATTTTGT CATCAAGACA 
ACCAATGTGA AAAAACCCAA ACTGACCATC TCCTATCCTG GTGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTTACCAA CCACCAGTAC CAGGAACTAA CAGAGCCTTC GTCTCTCACC 
TATGTCACCC GCTCTGAGAA CAGCATCTTT TTTGAAGTTG ATGGACCATA CCTTGCTATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAGCTGAAGA AAAGATATGC TGTATTCAAT 
GAAGATGGCT CCTTGGCTGA ACTGAAAGGT TTTGAGGTGA AACGCCGAGG GGAGTTGCAG 
TTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTCC TCAAGGGCAG CACACTAGAG 
GAGGTGTATG CCTCTGTGGC CAAAGTGGCT GACTACTGGC TAGATGTGCT CTATAGCAAG 
GCTGCTAATA TGCCCGATTC TGAATTATTC GAACTGATTT CTGAGAACCG CTCCATGTCA 
CGGAAGCTAG AAGATTATGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCTGAGTTCC TGGGAGACCA GATGGTCAAA GATGCTGGAC TGAGCTGCCG CTACATTATC 
TCCCGAAAGC CAGAGGGATC TCCTGTCACT GAGAGGGCCA TTCCACTTGC CATTTTCCAA 
GCAGAGCCTA CAGTGAGGAA ACATTTTCTC CGGAAATGGT TAAAGAGTTC ATCACTTCAA 
GACTTTGATA TTCGGACAAT TCTGGACTGG GACTACTACA TAGAGCGGCT GGGGAGTGCC 
ATCCAGAAAA TCATCACCAT CCCTGCAGCT CTACAGCAGG TGAAGAACCC AGTTCCACGT 
GTCAAACATC CAGACTGGCT GCACAAAAAG CTACTAGAGA AGAATGATAT CTACAAACAG 
AAGAAGATCA GTGAGCTCTT TGTGCTTGAA GGAAAGAGAC AGATTGTGAT GGCCCAGGCT 
TCAGAAAACA GTCTGAGTCT CTGCACTCCA GACATGGAGG ACTTTGGACT CACAAAGCCA 
TATCACTCTA CAGTTCCAGT TGCTACTAAG AGGAAGCGAG TCTGGGAGAG CCAAAAGGAG 
TCTCAGGATA TTACACTAAC TGTGCCCTGG CAAGAGGTCT TAGGGCAGCC TCCCTCCCTT 
GGAACCACAC AGGAAGAGTG GTTGGTCTGG CTCCAGTTCC ACAAGAAAAA GTGGCAGCTG 
CAGGCTCAAC AGCGCCTAGC CCGCAGGAAG AAACAGCGCT TAGAGTCAGC AGAAGATATG 
CCAAGGCTTG GGGCTATCCG AGAGGGGCCT GCCACAGGAC TGGGGAGCTT TCTGCGACGG 
ACCGCCCGCA GCATCATGGA CCTCCCCTGG CAGATCATAC AGATCAGTGA GACCAGACAG 
GCTGGTCTGT TCCGGCTGTG GGCTATCATT GGCAGCGACT TGCACTGCAT CAAGCTGAGT 
ATCCCTCGAG TATTCTATGT AAACCAGCGG GTAGCCAAAG CAGAGGATGG GCCTTCGTAT 
CGAAAGGTGA ATCGGGCTCT TCCTCGTTCC AACATCGTCT ACAATCTCTA TGAGTACTCA 
GTACCAGAGG ACATGTACCA AGAACACATC AACGAGATCA ACACTGAGTT GTCAGCACCA 
GACATTGAGG GCGTGTATGA GACTCAGGTC CCATTGTTAT TCCGGGCCCT GGTGCAGCTG 
GGCTGTGTGT GTGTGGTCAA CAAACAGCTG GCAAGGCAAC TTTCAGGCTG GGAAGCTGAA 
ACCTTTGCCC TCGAGCACCT TGAAATGCGT TCTCTGGCCC AGTTCAGCTA CTTGGAACCA 
GGGAGTATCC GCCACATCTA CCTGTACCAT CACACACAGG GCCACAAGGC ACTCTTTGGG 
GTCTTTATCC CCTCACAGCG AAGAGCATCT GTGTTTGTGT TGGATACTGT GCGCAGCAAC 
CAAATGCCAG GGCTCAGTGC CCTATACTCA GCAGAACACA GCCTGCTGCT GGACAAGGTG 
GGCCCCAACC TCCTGCCTCC CCCAAAACAC ACCTTTGAAG TTCGTGCTGA AACCAACCTG 
AAGACCATCT GCAGAGCCAT CCAACGCTTC CTGCTTGCCT ACAAGGAAGA GCGCCGGGGG 
CCCACGCTCA TCGCTATCCA GTCTAGCTGG GAACTGTGTA GGCTGACCAG TGAGATTCCA 
GTCTTAGAGG AGTTCCCACT AGTGCCTGTC CGAGTGGCTG ACAAGATCAG CTATGCAGTC 
CTGGACTGGC AGCGCCACGG AGCTCGCCGA ATGATCCGGC ACTACCTCAA TTTAGACATT 
TGCCTTTCAC AGGCCTTTGA GATGAGCAGG TACTTCCACA TCCCCGTTGG AAACCTGCCA 
GAAGACATCT CCACCTTTGG CTCTGACCTC TTTTTTGCAC GCCATCTCCA GCGCCATAAC 
CACCTACTTT GGTTGTCCCC TACCTCTCGG CCTGACCTGG GTGGGAAGGA AGCTGATGAC 
AACCGCCTTG TCATGGAGTT TGATGACCGA GCCACTGTGG AGATCAATAA TTCCGGCTGT 
TACTCTACTG TGTGCGTGGA GCTGGACATT CAAAACCTGG CAGTCAACAC CATCCTCCAG 
TCCCACCATG TCAATGACAT GGAGGGGGCT GGCAGCATGG GCATCAGCTT CGATGTGATC 
CAGCAGGCCT CCCTAGAGGA CATGGTTACA GGCAATCAAG CTGCCAGTGC CCTGGCCAGC 
TACGATGAGA CAGCCCTCTG CTCCAGTACG TTCAGGATCC TGAAGAGCAT GGTGGTTGGC 
TGGGTAAAGG AAATCACACA GTACCACAAC ATCTATGCTG ACAACCAGGT GATGCACTTC 
TACCGCTGGC TCCAGTCGCC GTGCTCTCTG CTCCACGACC CAGCCCTTCA CCGGACACTG 
CACAATATGA TGAAGAAGCT CTTCCTGCAG CTCATTGCTG AGTTCAAGCG CCTGGGGTCA 
TCGGTCATCT ATGCCAACTT CAATCGCATC ATCCTCTGTA CAAAGAAGCG CCGGATAGAG 
GATGCCCTTG CCTATGTGGA ATATATCACC AACAGCATCC ATTCTAAAGA GATCTTTCAT 
TCCCTGACCA TCTCTTTCTC TCGATGCTGG GAATTCCTTC TCTGGATGGA TCCATCCAAC 
TATGGTGGAA TCAAAGGAAA AGTCCCATCC AGTATTCACT GTGGACAAGT AAGGGAGCAA 
GACACCCTGG GAACTGGGCA GCTCAGGCAC ATTGGGTGTT TGGGCAGTAG TTTCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pole ( NM_001107152.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100622.2 Rattus norvegicus DNA polymerase epsilon, catalytic subunit (Pole), mRNA.

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CloneID	ORa05209
Clone ID Related Accession (Same CDS sequence)	NM_001107152.2
Accession Version	NM_001107152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1570636800000
Organism	Rattus norvegicus(Norway rat)
Product	DNA polymerase epsilon catalytic subunit A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC120688.5. On Nov 30, 2010 this sequence version replaced NM_001107152.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based onpartial transcripts and orthologous alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCTGA GGAACAGTGG ACGGAGGCAT CCCGAGCCGG GCGCGGACAG CGAAGGCAGC 
CGGGATGATG GTCCCTCCTC TTCAGTCTCA GCACTCAAGC GTCTGGAACG GAGCCAGTGG 
ACGGATAAGA TGGACTTACG GTTTGGTTTT GAAAGGTTGA AAGAGCCTGG AGAAAGGACT 
GGCTGGCTTA TCAACATGCA CCCTACAGAG ATTTTAGATG AAGACAAACG CTTAGTCAGT 
GCAGTGGATT ACTACTTCAT TCAAGATGAT GGAAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC AGCTAGAAAG GGTTGTGATC GAGAAGTTTC ATCTTTTCTA 
TCCAAGAAGT TTCAGGGTAA AATTGCAAAG TTAGAGAATG TGCCCAAAGA AGACCTGGAC 
TTGCCAAACC ACTTGGTGGG CTTGAAGCGG AGTTACATCA AGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG AAAGGAGATC TCTCCTGCTG TGAAGAAGAA CCGAGAGCAG 
GACCATGCTA GTGACGAGTA CACAACAATG CTCTCCAGTA TTCTGCAAGG AGGCAGTCTA 
ATTACTGATG AGGAGGAAAC CTCTAAGAAG ATAGCTGACC AATTGGACAA CATAGTGGAC 
ATGCGGGAGT ATGATGTTCC CTACCACATT CGCCTCTCCA TTGACCTCAG AATCCATGTG 
GCCCACTGGT ACAATGTTAG ATTTCGAGGA AATGCTTTTC CTGTGGAAAT TGCCCGACGA 
GATGATCTCG TCGAACGACC TGATCCTGTG GTTTTGGCAT TTGACATTGA GACAACCAAA 
CTACCTCTCA AATTCCCTGA TGCTGAGACA GATCAGATTA TGATGATCTC CTATATGATT 
GATGGCCAGG GCTACCTCAT CACTAACAGG GAGATTGTTT CAGAAGACAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGG CCTTTCTGTG TCTTCAATGA ACCTGATGAG 
GTCCATCTGA TCCAGAGATG GTTTGAGCAT ATCCAGGAGA CCAAACCTAC CATTATGGTC 
ACCTACAATG GGGATTTTTT TGACTGGCCG TTTGTGGAGG CCAGGGCAGC AATTCATGGC 
CTAAGCATGT ACCAGGAGAT AGGCTTCCAG AAGGATAGCC AGGGGGAATA TAAGGCACCA 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCAGC TGCCAAAGCC AAACTTGGCT ATGACCCTGT GGAGCTGGAC 
CCTGAGGACA TGTGCCGTAT GGCCACTGAA CAGCCCCAGA CTCTGGCCAC TTACTCAGTG 
TCAGATGCTG TTGCTACTTA TTACCTGTAC ATGAAATATG TGCACCCCTT CATATTCGCC 
CTCTGCACCA TTATTCCCAT GGAACCTGAT GAGGTGCTGC GGAAGGGCTC TGGAACATTG 
TGCGAAGCCT TGCTGATGGT GCAAGCCTTC CATGCCAACA TTATCTTTCC CAATAAGCAA 
GAGCAGGAGT TCAACAAGCT GACAGATGAT GGCCACGTGC TAGATGCTGA GACCTACGTT 
GGGGGCCATG TGGAGGCCCT AGAGTCTGGT GTCTTCCGAA GTGATATCCC CTGCCGGTTT 
AGGATGAATC CTGCAGCCTT TGATTTCCTG CTGCAGCGAG TTGAGAAGAC TATGCGCCAT 
GCCATTGAAG AAGAAGAGAA GGTGCCTGTG GAACAAGCCA CCAACTTCCA AGAGGTGTGT 
GAGCAGATTA AGACCAAACT CACCTCCCTA AAAGATGTTC CTAACAGAAT TGAATGTCCT 
CTAATCTACC ATCTAGATGT GGGGGCCATG TATCCTAACA TAATCCTCAC CAACCGCCTG 
CAGCCTTCTG CCATAGTGGA TGAGGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAACTTGTC AGAGGAAAAT GGCCTGGCAA TGGAGGGGAG AATTCATGCC AGCCAGTCGT 
AGTGAGTACC ATCGAATCCA GCATCAGCTA GAGTCGGAGA AGTTCCCCCC TTTGTTCCCA 
GAGGGGCCAG CAAGGGCCTT TCATGAGCTG TCCCGTGAAG AACAGGCTAA ATACGAGAAA 
CGGAGGCTGG CAGATTATTG CCGGAAAGCC TATAAGAAGA TCCATATGAC CAAGGTAGAA 
GAGCGCCTGA CTACCATCTG CCAGCGGGAA AACTCATTTT ATGTGGACAC AGTGCGGGCC 
TTCAGAGACA GGCGCTATGA ATTCAAAGGA CTGCACAAGG TGTGGAAGAA GAAGCTCTCG 
GCGGCTGTAG AGGTGGGCGA TGCATCAGAG GTGAAGCGCT GCAAGAACAT GGAGATCCTT 
TATGATTCGC TGCAGCTGGC TCACAAATGC ATCCTGAACT CATTCTACGG CTATGTTATG 
CGCAAAGGAG CTCGCTGGTA CTCCATGGAG ATGGCTGGTA TCGTCTGCTT TACAGGAGCC 
AACATCATCA CCCAGGCAAG AGAGCTGATT GAGCAGATCG GGAGGCCTTT AGAACTGGAC 
ACGGACGGAA TATGGTGCGT TCTACCCAAT AGCTTTCCTG AAAATTTTGT CATCAAGACA 
ACCAATGTGA AAAAACCCAA ACTGACCATC TCCTATCCTG GTGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTTACCAA CCACCAGTAC CAGGAACTAA CAGAGCCTTC GTCTCTCACC 
TATGTCACCC GCTCTGAGAA CAGCATCTTT TTTGAAGTTG ATGGACCATA CCTTGCTATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAGCTGAAGA AAAGATATGC TGTATTCAAT 
GAAGATGGCT CCTTGGCTGA ACTGAAAGGT TTTGAGGTGA AACGCCGAGG GGAGTTGCAG 
TTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTCC TCAAGGGCAG CACACTAGAG 
GAGGTGTATG CCTCTGTGGC CAAAGTGGCT GACTACTGGC TAGATGTGCT CTATAGCAAG 
GCTGCTAATA TGCCCGATTC TGAATTATTC GAACTGATTT CTGAGAACCG CTCCATGTCA 
CGGAAGCTAG AAGATTATGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCTGAGTTCC TGGGAGACCA GATGGTCAAA GATGCTGGAC TGAGCTGCCG CTACATTATC 
TCCCGAAAGC CAGAGGGATC TCCTGTCACT GAGAGGGCCA TTCCACTTGC CATTTTCCAA 
GCAGAGCCTA CAGTGAGGAA ACATTTTCTC CGGAAATGGT TAAAGAGTTC ATCACTTCAA 
GACTTTGATA TTCGGACAAT TCTGGACTGG GACTACTACA TAGAGCGGCT GGGGAGTGCC 
ATCCAGAAAA TCATCACCAT CCCTGCAGCT CTACAGCAGG TGAAGAACCC AGTTCCACGT 
GTCAAACATC CAGACTGGCT GCACAAAAAG CTACTAGAGA AGAATGATAT CTACAAACAG 
AAGAAGATCA GTGAGCTCTT TGTGCTTGAA GGAAAGAGAC AGATTGTGAT GGCCCAGGCT 
TCAGAAAACA GTCTGAGTCT CTGCACTCCA GACATGGAGG ACTTTGGACT CACAAAGCCA 
TATCACTCTA CAGTTCCAGT TGCTACTAAG AGGAAGCGAG TCTGGGAGAG CCAAAAGGAG 
TCTCAGGATA TTACACTAAC TGTGCCCTGG CAAGAGGTCT TAGGGCAGCC TCCCTCCCTT 
GGAACCACAC AGGAAGAGTG GTTGGTCTGG CTCCAGTTCC ACAAGAAAAA GTGGCAGCTG 
CAGGCTCAAC AGCGCCTAGC CCGCAGGAAG AAACAGCGCT TAGAGTCAGC AGAAGATATG 
CCAAGGCTTG GGGCTATCCG AGAGGGGCCT GCCACAGGAC TGGGGAGCTT TCTGCGACGG 
ACCGCCCGCA GCATCATGGA CCTCCCCTGG CAGATCATAC AGATCAGTGA GACCAGACAG 
GCTGGTCTGT TCCGGCTGTG GGCTATCATT GGCAGCGACT TGCACTGCAT CAAGCTGAGT 
ATCCCTCGAG TATTCTATGT AAACCAGCGG GTAGCCAAAG CAGAGGATGG GCCTTCGTAT 
CGAAAGGTGA ATCGGGCTCT TCCTCGTTCC AACATCGTCT ACAATCTCTA TGAGTACTCA 
GTACCAGAGG ACATGTACCA AGAACACATC AACGAGATCA ACACTGAGTT GTCAGCACCA 
GACATTGAGG GCGTGTATGA GACTCAGGTC CCATTGTTAT TCCGGGCCCT GGTGCAGCTG 
GGCTGTGTGT GTGTGGTCAA CAAACAGCTG GCAAGGCAAC TTTCAGGCTG GGAAGCTGAA 
ACCTTTGCCC TCGAGCACCT TGAAATGCGT TCTCTGGCCC AGTTCAGCTA CTTGGAACCA 
GGGAGTATCC GCCACATCTA CCTGTACCAT CACACACAGG GCCACAAGGC ACTCTTTGGG 
GTCTTTATCC CCTCACAGCG AAGAGCATCT GTGTTTGTGT TGGATACTGT GCGCAGCAAC 
CAAATGCCAG GGCTCAGTGC CCTATACTCA GCAGAACACA GCCTGCTGCT GGACAAGGTG 
GGCCCCAACC TCCTGCCTCC CCCAAAACAC ACCTTTGAAG TTCGTGCTGA AACCAACCTG 
AAGACCATCT GCAGAGCCAT CCAACGCTTC CTGCTTGCCT ACAAGGAAGA GCGCCGGGGG 
CCCACGCTCA TCGCTATCCA GTCTAGCTGG GAACTGTGTA GGCTGACCAG TGAGATTCCA 
GTCTTAGAGG AGTTCCCACT AGTGCCTGTC CGAGTGGCTG ACAAGATCAG CTATGCAGTC 
CTGGACTGGC AGCGCCACGG AGCTCGCCGA ATGATCCGGC ACTACCTCAA TTTAGACATT 
TGCCTTTCAC AGGCCTTTGA GATGAGCAGG TACTTCCACA TCCCCGTTGG AAACCTGCCA 
GAAGACATCT CCACCTTTGG CTCTGACCTC TTTTTTGCAC GCCATCTCCA GCGCCATAAC 
CACCTACTTT GGTTGTCCCC TACCTCTCGG CCTGACCTGG GTGGGAAGGA AGCTGATGAC 
AACCGCCTTG TCATGGAGTT TGATGACCGA GCCACTGTGG AGATCAATAA TTCCGGCTGT 
TACTCTACTG TGTGCGTGGA GCTGGACATT CAAAACCTGG CAGTCAACAC CATCCTCCAG 
TCCCACCATG TCAATGACAT GGAGGGGGCT GGCAGCATGG GCATCAGCTT CGATGTGATC 
CAGCAGGCCT CCCTAGAGGA CATGGTTACA GGCAATCAAG CTGCCAGTGC CCTGGCCAGC 
TACGATGAGA CAGCCCTCTG CTCCAGTACG TTCAGGATCC TGAAGAGCAT GGTGGTTGGC 
TGGGTAAAGG AAATCACACA GTACCACAAC ATCTATGCTG ACAACCAGGT GATGCACTTC 
TACCGCTGGC TCCAGTCGCC GTGCTCTCTG CTCCACGACC CAGCCCTTCA CCGGACACTG 
CACAATATGA TGAAGAAGCT CTTCCTGCAG CTCATTGCTG AGTTCAAGCG CCTGGGGTCA 
TCGGTCATCT ATGCCAACTT CAATCGCATC ATCCTCTGTA CAAAGAAGCG CCGGATAGAG 
GATGCCCTTG CCTATGTGGA ATATATCACC AACAGCATCC ATTCTAAAGA GATCTTTCAT 
TCCCTGACCA TCTCTTTCTC TCGATGCTGG GAATTCCTTC TCTGGATGGA TCCATCCAAC 
TATGGTGGAA TCAAAGGAAA AGTCCCATCC AGTATTCACT GTGGACAAGT AAGGGAGCAA 
GACTCCCAGA CAAGGGAGGA AACTGAGGAT GAGGAGGACA ATGAAAAGGA TGAGGAGGAA 
GAGGAGGACA TGGGGCAATC TGAGGTTGAG GACTTACTGG AGAACAACTG GAACATTCTA 
CAGTTCTTGC CACAGGCAGC CTCTTGCCAG AGCTACTTCC TTATGATCGT TTCAGCATAC 
ATCGTGGCTG TGTACCAAAG CATGAAGGAT GAGTTGAGAC ACAGTGCCCC AGGCAGTACC 
CCTGTGAAGA GGAAGGGGGC CAGCCAGTTC TCCCAGGAGG CTGAAGGGGC AGCCGGGGCC 
CTTCCTGGAA TGATCACTTT CTCTCAAGAT TATGTGGCAA ATGAGCTCAC TCAGACCTTC 
TTCACCATTA CTCAGAAAAT TCAGAAGAAA GTCACAGGCT CTCGGAACAC CGCTGAGCCC 
TCAGAGATGT TCCCTGTCCT CCCTGGTTCA CATTTGCTGC TCAATAACCC TGCTCTGGAG 
TTCATCAAAT ACGTGTGCAA GGTACTATCT CTGGATACAA ACATCACAAA TCAGGTGAAT 
AAGCTAAACA GAGACCTGCT TCGCCTGGTA GACGTTGGTG AATTCTCTGA GGAGGCCCAG 
TTCAGAGACC CCTGCCACTC CTACGTGCTC CCTGAGGTAA TCTGCCACAG CTGTAATTTC 
TGCCGAGACC TGGACCTATG CAAAGATTCT TCTTTCTCTC AGGACGGAGC CATCCTGCCT 
CAGTGGCTTT GCTCCAATTG TCAGGCCCCC TACGACTCAT CTGCCATTGA GTCAGCCTTG 
GTGGAAGCCC TGCAGAGGAA ACTGATGGCC TTCACACTTC AGGACCTGGT GTGCCTCAAG 
TGCCGTGGTA TGAAAGAGAC CCATATGCCT GTGTACTGCG GCTGCGCAGG GGACTTTGTT 
CTCACCATCC ATACTGAGGT CTTCATGGAA CAGATTAGAA TCTTCCAGAA CATTGCCAAG 
TACTACAACA TGTCATATCT CCAAGAGGCC ATAGAATGGC TGCTACAGAC AAGCCCTGTA 
TCAAACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100622.2
CDS	44..6895
Translation	MVLRNSGRRHPEPGADSEGSRDDGPSSSVSALKRLERSQWTDKMDLRFGFERLKEPGERTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYKPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLDLPNHLVGLKRSYIKLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSLITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEIARRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEHIQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAIVDEATCAACDFNKPGATCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHMTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNVKKPKLTISYPGAMLNIMVKEGFTNHQYQELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYASVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDIYKQKKISELFVLEGKRQIVMAQASENSLSLCTPDMEDFGLTKPYHSTVPVATKRKRVWESQKESQDITLTVPWQEVLGQPPSLGTTQEEWLVWLQFHKKKWQLQAQQRLARRKKQRLESAEDMPRLGAIREGPATGLGSFLRRTARSIMDLPWQIIQISETRQAGLFRLWAIIGSDLHCIKLSIPRVFYVNQRVAKAEDGPSYRKVNRALPRSNIVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLARQLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHTQGHKALFGVFIPSQRRASVFVLDTVRSNQMPGLSALYSAEHSLLLDKVGPNLLPPPKHTFEVRAETNLKTICRAIQRFLLAYKEERRGPTLIAIQSSWELCRLTSEIPVLEEFPLVPVRVADKISYAVLDWQRHGARRMIRHYLNLDICLSQAFEMSRYFHIPVGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDRATVEINNSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGAGSMGISFDVIQQASLEDMVTGNQAASALASYDETALCSSTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLQSPCSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRIEDALAYVEYITNSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGKVPSSIHCGQVREQDSQTREETEDEEDNEKDEEEEEDMGQSEVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYQSMKDELRHSAPGSTPVKRKGASQFSQEAEGAAGALPGMITFSQDYVANELTQTFFTITQKIQKKVTGSRNTAEPSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCHSYVLPEVICHSCNFCRDLDLCKDSSFSQDGAILPQWLCSNCQAPYDSSAIESALVEALQRKLMAFTLQDLVCLKCRGMKETHMPVYCGCAGDFVLTIHTEVFMEQIRIFQNIAKYYNMSYLQEAIEWLLQTSPVSNF

Target ORF information:

RefSeq Version	NM_001107152.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA polymerase epsilon, catalytic subunit (Pole), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107152.2

ORF Insert Sequence:

ATGGTGCTGA GGAACAGTGG ACGGAGGCAT CCCGAGCCGG GCGCGGACAG CGAAGGCAGC 
CGGGATGATG GTCCCTCCTC TTCAGTCTCA GCACTCAAGC GTCTGGAACG GAGCCAGTGG 
ACGGATAAGA TGGACTTACG GTTTGGTTTT GAAAGGTTGA AAGAGCCTGG AGAAAGGACT 
GGCTGGCTTA TCAACATGCA CCCTACAGAG ATTTTAGATG AAGACAAACG CTTAGTCAGT 
GCAGTGGATT ACTACTTCAT TCAAGATGAT GGAAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC AGCTAGAAAG GGTTGTGATC GAGAAGTTTC ATCTTTTCTA 
TCCAAGAAGT TTCAGGGTAA AATTGCAAAG TTAGAGAATG TGCCCAAAGA AGACCTGGAC 
TTGCCAAACC ACTTGGTGGG CTTGAAGCGG AGTTACATCA AGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG AAAGGAGATC TCTCCTGCTG TGAAGAAGAA CCGAGAGCAG 
GACCATGCTA GTGACGAGTA CACAACAATG CTCTCCAGTA TTCTGCAAGG AGGCAGTCTA 
ATTACTGATG AGGAGGAAAC CTCTAAGAAG ATAGCTGACC AATTGGACAA CATAGTGGAC 
ATGCGGGAGT ATGATGTTCC CTACCACATT CGCCTCTCCA TTGACCTCAG AATCCATGTG 
GCCCACTGGT ACAATGTTAG ATTTCGAGGA AATGCTTTTC CTGTGGAAAT TGCCCGACGA 
GATGATCTCG TCGAACGACC TGATCCTGTG GTTTTGGCAT TTGACATTGA GACAACCAAA 
CTACCTCTCA AATTCCCTGA TGCTGAGACA GATCAGATTA TGATGATCTC CTATATGATT 
GATGGCCAGG GCTACCTCAT CACTAACAGG GAGATTGTTT CAGAAGACAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGG CCTTTCTGTG TCTTCAATGA ACCTGATGAG 
GTCCATCTGA TCCAGAGATG GTTTGAGCAT ATCCAGGAGA CCAAACCTAC CATTATGGTC 
ACCTACAATG GGGATTTTTT TGACTGGCCG TTTGTGGAGG CCAGGGCAGC AATTCATGGC 
CTAAGCATGT ACCAGGAGAT AGGCTTCCAG AAGGATAGCC AGGGGGAATA TAAGGCACCA 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCAGC TGCCAAAGCC AAACTTGGCT ATGACCCTGT GGAGCTGGAC 
CCTGAGGACA TGTGCCGTAT GGCCACTGAA CAGCCCCAGA CTCTGGCCAC TTACTCAGTG 
TCAGATGCTG TTGCTACTTA TTACCTGTAC ATGAAATATG TGCACCCCTT CATATTCGCC 
CTCTGCACCA TTATTCCCAT GGAACCTGAT GAGGTGCTGC GGAAGGGCTC TGGAACATTG 
TGCGAAGCCT TGCTGATGGT GCAAGCCTTC CATGCCAACA TTATCTTTCC CAATAAGCAA 
GAGCAGGAGT TCAACAAGCT GACAGATGAT GGCCACGTGC TAGATGCTGA GACCTACGTT 
GGGGGCCATG TGGAGGCCCT AGAGTCTGGT GTCTTCCGAA GTGATATCCC CTGCCGGTTT 
AGGATGAATC CTGCAGCCTT TGATTTCCTG CTGCAGCGAG TTGAGAAGAC TATGCGCCAT 
GCCATTGAAG AAGAAGAGAA GGTGCCTGTG GAACAAGCCA CCAACTTCCA AGAGGTGTGT 
GAGCAGATTA AGACCAAACT CACCTCCCTA AAAGATGTTC CTAACAGAAT TGAATGTCCT 
CTAATCTACC ATCTAGATGT GGGGGCCATG TATCCTAACA TAATCCTCAC CAACCGCCTG 
CAGCCTTCTG CCATAGTGGA TGAGGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAACTTGTC AGAGGAAAAT GGCCTGGCAA TGGAGGGGAG AATTCATGCC AGCCAGTCGT 
AGTGAGTACC ATCGAATCCA GCATCAGCTA GAGTCGGAGA AGTTCCCCCC TTTGTTCCCA 
GAGGGGCCAG CAAGGGCCTT TCATGAGCTG TCCCGTGAAG AACAGGCTAA ATACGAGAAA 
CGGAGGCTGG CAGATTATTG CCGGAAAGCC TATAAGAAGA TCCATATGAC CAAGGTAGAA 
GAGCGCCTGA CTACCATCTG CCAGCGGGAA AACTCATTTT ATGTGGACAC AGTGCGGGCC 
TTCAGAGACA GGCGCTATGA ATTCAAAGGA CTGCACAAGG TGTGGAAGAA GAAGCTCTCG 
GCGGCTGTAG AGGTGGGCGA TGCATCAGAG GTGAAGCGCT GCAAGAACAT GGAGATCCTT 
TATGATTCGC TGCAGCTGGC TCACAAATGC ATCCTGAACT CATTCTACGG CTATGTTATG 
CGCAAAGGAG CTCGCTGGTA CTCCATGGAG ATGGCTGGTA TCGTCTGCTT TACAGGAGCC 
AACATCATCA CCCAGGCAAG AGAGCTGATT GAGCAGATCG GGAGGCCTTT AGAACTGGAC 
ACGGACGGAA TATGGTGCGT TCTACCCAAT AGCTTTCCTG AAAATTTTGT CATCAAGACA 
ACCAATGTGA AAAAACCCAA ACTGACCATC TCCTATCCTG GTGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTTACCAA CCACCAGTAC CAGGAACTAA CAGAGCCTTC GTCTCTCACC 
TATGTCACCC GCTCTGAGAA CAGCATCTTT TTTGAAGTTG ATGGACCATA CCTTGCTATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAGCTGAAGA AAAGATATGC TGTATTCAAT 
GAAGATGGCT CCTTGGCTGA ACTGAAAGGT TTTGAGGTGA AACGCCGAGG GGAGTTGCAG 
TTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTCC TCAAGGGCAG CACACTAGAG 
GAGGTGTATG CCTCTGTGGC CAAAGTGGCT GACTACTGGC TAGATGTGCT CTATAGCAAG 
GCTGCTAATA TGCCCGATTC TGAATTATTC GAACTGATTT CTGAGAACCG CTCCATGTCA 
CGGAAGCTAG AAGATTATGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCTGAGTTCC TGGGAGACCA GATGGTCAAA GATGCTGGAC TGAGCTGCCG CTACATTATC 
TCCCGAAAGC CAGAGGGATC TCCTGTCACT GAGAGGGCCA TTCCACTTGC CATTTTCCAA 
GCAGAGCCTA CAGTGAGGAA ACATTTTCTC CGGAAATGGT TAAAGAGTTC ATCACTTCAA 
GACTTTGATA TTCGGACAAT TCTGGACTGG GACTACTACA TAGAGCGGCT GGGGAGTGCC 
ATCCAGAAAA TCATCACCAT CCCTGCAGCT CTACAGCAGG TGAAGAACCC AGTTCCACGT 
GTCAAACATC CAGACTGGCT GCACAAAAAG CTACTAGAGA AGAATGATAT CTACAAACAG 
AAGAAGATCA GTGAGCTCTT TGTGCTTGAA GGAAAGAGAC AGATTGTGAT GGCCCAGGCT 
TCAGAAAACA GTCTGAGTCT CTGCACTCCA GACATGGAGG ACTTTGGACT CACAAAGCCA 
TATCACTCTA CAGTTCCAGT TGCTACTAAG AGGAAGCGAG TCTGGGAGAG CCAAAAGGAG 
TCTCAGGATA TTACACTAAC TGTGCCCTGG CAAGAGGTCT TAGGGCAGCC TCCCTCCCTT 
GGAACCACAC AGGAAGAGTG GTTGGTCTGG CTCCAGTTCC ACAAGAAAAA GTGGCAGCTG 
CAGGCTCAAC AGCGCCTAGC CCGCAGGAAG AAACAGCGCT TAGAGTCAGC AGAAGATATG 
CCAAGGCTTG GGGCTATCCG AGAGGGGCCT GCCACAGGAC TGGGGAGCTT TCTGCGACGG 
ACCGCCCGCA GCATCATGGA CCTCCCCTGG CAGATCATAC AGATCAGTGA GACCAGACAG 
GCTGGTCTGT TCCGGCTGTG GGCTATCATT GGCAGCGACT TGCACTGCAT CAAGCTGAGT 
ATCCCTCGAG TATTCTATGT AAACCAGCGG GTAGCCAAAG CAGAGGATGG GCCTTCGTAT 
CGAAAGGTGA ATCGGGCTCT TCCTCGTTCC AACATCGTCT ACAATCTCTA TGAGTACTCA 
GTACCAGAGG ACATGTACCA AGAACACATC AACGAGATCA ACACTGAGTT GTCAGCACCA 
GACATTGAGG GCGTGTATGA GACTCAGGTC CCATTGTTAT TCCGGGCCCT GGTGCAGCTG 
GGCTGTGTGT GTGTGGTCAA CAAACAGCTG GCAAGGCAAC TTTCAGGCTG GGAAGCTGAA 
ACCTTTGCCC TCGAGCACCT TGAAATGCGT TCTCTGGCCC AGTTCAGCTA CTTGGAACCA 
GGGAGTATCC GCCACATCTA CCTGTACCAT CACACACAGG GCCACAAGGC ACTCTTTGGG 
GTCTTTATCC CCTCACAGCG AAGAGCATCT GTGTTTGTGT TGGATACTGT GCGCAGCAAC 
CAAATGCCAG GGCTCAGTGC CCTATACTCA GCAGAACACA GCCTGCTGCT GGACAAGGTG 
GGCCCCAACC TCCTGCCTCC CCCAAAACAC ACCTTTGAAG TTCGTGCTGA AACCAACCTG 
AAGACCATCT GCAGAGCCAT CCAACGCTTC CTGCTTGCCT ACAAGGAAGA GCGCCGGGGG 
CCCACGCTCA TCGCTATCCA GTCTAGCTGG GAACTGTGTA GGCTGACCAG TGAGATTCCA 
GTCTTAGAGG AGTTCCCACT AGTGCCTGTC CGAGTGGCTG ACAAGATCAG CTATGCAGTC 
CTGGACTGGC AGCGCCACGG AGCTCGCCGA ATGATCCGGC ACTACCTCAA TTTAGACATT 
TGCCTTTCAC AGGCCTTTGA GATGAGCAGG TACTTCCACA TCCCCGTTGG AAACCTGCCA 
GAAGACATCT CCACCTTTGG CTCTGACCTC TTTTTTGCAC GCCATCTCCA GCGCCATAAC 
CACCTACTTT GGTTGTCCCC TACCTCTCGG CCTGACCTGG GTGGGAAGGA AGCTGATGAC 
AACCGCCTTG TCATGGAGTT TGATGACCGA GCCACTGTGG AGATCAATAA TTCCGGCTGT 
TACTCTACTG TGTGCGTGGA GCTGGACATT CAAAACCTGG CAGTCAACAC CATCCTCCAG 
TCCCACCATG TCAATGACAT GGAGGGGGCT GGCAGCATGG GCATCAGCTT CGATGTGATC 
CAGCAGGCCT CCCTAGAGGA CATGGTTACA GGCAATCAAG CTGCCAGTGC CCTGGCCAGC 
TACGATGAGA CAGCCCTCTG CTCCAGTACG TTCAGGATCC TGAAGAGCAT GGTGGTTGGC 
TGGGTAAAGG AAATCACACA GTACCACAAC ATCTATGCTG ACAACCAGGT GATGCACTTC 
TACCGCTGGC TCCAGTCGCC GTGCTCTCTG CTCCACGACC CAGCCCTTCA CCGGACACTG 
CACAATATGA TGAAGAAGCT CTTCCTGCAG CTCATTGCTG AGTTCAAGCG CCTGGGGTCA 
TCGGTCATCT ATGCCAACTT CAATCGCATC ATCCTCTGTA CAAAGAAGCG CCGGATAGAG 
GATGCCCTTG CCTATGTGGA ATATATCACC AACAGCATCC ATTCTAAAGA GATCTTTCAT 
TCCCTGACCA TCTCTTTCTC TCGATGCTGG GAATTCCTTC TCTGGATGGA TCCATCCAAC 
TATGGTGGAA TCAAAGGAAA AGTCCCATCC AGTATTCACT GTGGACAAGT AAGGGAGCAA 
GACTCCCAGA CAAGGGAGGA AACTGAGGAT GAGGAGGACA ATGAAAAGGA TGAGGAGGAA 
GAGGAGGACA TGGGGCAATC TGAGGTTGAG GACTTACTGG AGAACAACTG GAACATTCTA 
CAGTTCTTGC CACAGGCAGC CTCTTGCCAG AGCTACTTCC TTATGATCGT TTCAGCATAC 
ATCGTGGCTG TGTACCAAAG CATGAAGGAT GAGTTGAGAC ACAGTGCCCC AGGCAGTACC 
CCTGTGAAGA GGAAGGGGGC CAGCCAGTTC TCCCAGGAGG CTGAAGGGGC AGCCGGGGCC 
CTTCCTGGAA TGATCACTTT CTCTCAAGAT TATGTGGCAA ATGAGCTCAC TCAGACCTTC 
TTCACCATTA CTCAGAAAAT TCAGAAGAAA GTCACAGGCT CTCGGAACAC CGCTGAGCCC 
TCAGAGATGT TCCCTGTCCT CCCTGGTTCA CATTTGCTGC TCAATAACCC TGCTCTGGAG 
TTCATCAAAT ACGTGTGCAA GGTACTATCT CTGGATACAA ACATCACAAA TCAGGTGAAT 
AAGCTAAACA GAGACCTGCT TCGCCTGGTA GACGTTGGTG AATTCTCTGA GGAGGCCCAG 
TTCAGAGACC CCTGCCACTC CTACGTGCTC CCTGAGGTAA TCTGCCACAG CTGTAATTTC 
TGCCGAGACC TGGACCTATG CAAAGATTCT TCTTTCTCTC AGGACGGAGC CATCCTGCCT 
CAGTGGCTTT GCTCCAATTG TCAGGCCCCC TACGACTCAT CTGCCATTGA GTCAGCCTTG 
GTGGAAGCCC TGCAGAGGAA ACTGATGGCC TTCACACTTC AGGACCTGGT GTGCCTCAAG 
TGCCGTGGTA TGAAAGAGAC CCATATGCCT GTGTACTGCG GCTGCGCAGG GGACTTTGTT 
CTCACCATCC ATACTGAGGT CTTCATGGAA CAGATTAGAA TCTTCCAGAA CATTGCCAAG 
TACTACAACA TGTCATATCT CCAAGAGGCC ATAGAATGGC TGCTACAGAC AAGCCCTGTA 
TCAAACTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA polymerase δ and ζ switch by sharing accessory subunits of DNA polymerase δ.
The Journal of biological chemistry287(21)17281-7(2012 May)
Baranovskiy AG,Lada AG,Siebler HM,Zhang Y,Pavlov YI,Tahirov TH

DNA polymerases and repair synthesis in NER in human cells.
DNA repair10(7)730-3(2011 Jul)
Lehmann AR

Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells.
Molecular cell37(5)714-27(2010 Mar)
Ogi T,Limsirichaikul S,Overmeer RM,Volker M,Takenaka K,Cloney R,Nakazawa Y,Niimi A,Miki Y,Jaspers NG,Mullenders LH,Yamashita S,Fousteri MI,Lehmann AR

Distinctive activities of DNA polymerases during human DNA replication.
The FEBS journal273(13)2984-3001(2006 Jul)
Rytkönen AK,Vaara M,Nethanel T,Kaufmann G,Sormunen R,Läärä E,Nasheuer HP,Rahmeh A,Lee MY,Syväoja JE,Pospiech H

DNA polymerase epsilon - more than a polymerase.
TheScientificWorldJournal387-104(2003 Mar)
Pospiech H,Syväoja JE

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Pole cDNA ORF clone, Rattus norvegicus(Norway rat)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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