POLE cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLE
Entrez Gene ID	785457
Full Name	DNA polymerase epsilon, catalytic subunit
General protein information	Preferred Names DNA polymerase epsilon, catalytic subunit Names DNA polymerase epsilon catalytic subunit A polymerase (DNA directed), epsilon, catalytic subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005217684.4	XP_005217741.1	DNA polymerase epsilon catalytic subunit A isoform X1
NM_001191429.1	NP_001178358.1	DNA polymerase epsilon catalytic subunit A
XM_024977498.1	XP_024833266.1	DNA polymerase epsilon catalytic subunit A isoform X2
XM_005217684.3	XP_005217741.1	DNA polymerase epsilon catalytic subunit A isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36974	NM_001191429.1 Latest version!	Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa224428	XM_005217684.4 Latest version!	Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa224429	XM_024977498.1 Latest version!	Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa27766	XM_005217684.3 Latest version!	Bos taurus polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLE ( NM_001191429.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178358.1 Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), mRNA.

Price & Availability↑

CloneID	OBa36974
Clone ID Related Accession (Same CDS sequence)	NM_001191429.1
Accession Version	NM_001191429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1508601600000
Organism	Bos taurus(cattle)
Product	DNA polymerase epsilon catalytic subunit A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02044945.1 and DAAA02044946.1. On Apr 21, 2011 this sequence version replaced XM_002701687.1. Sequence Note: This RefSeq record was created from genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on full-length homologous protein alignment with human NP_006222.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822095 [ECO:0000348] ##Evidence-Data-END##

ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG 
ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT 
GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT 
TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC 
TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG 
GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG 
AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA 
ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC 
ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG 
GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT 
GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA 
CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT 
GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC 
GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG 
GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC 
ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA 
CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC 
CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC 
AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC 
CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG 
TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC 
CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG 
TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG 
GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG 
GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC 
CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT 
GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT 
GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG 
CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA 
GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC 
AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC 
GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG 
CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT 
TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC 
GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG 
TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG 
CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC 
ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG 
GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC 
TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT 
GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG 
CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA 
GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG 
GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG 
GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC 
TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG 
GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG 
GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC 
ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT 
GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT 
TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA 
CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG 
GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA 
CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC 
ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA 
AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC 
CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG 
AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC 
TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC 
TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG 
CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG 
CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA 
CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT 
GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC 
AACCAGATGC CCAGCCTCAG TGCCTTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA 
GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC 
TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT 
GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC 
CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG 
GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC 
ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG 
CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC 
GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC 
TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG 
CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC 
AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG 
GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG 
CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG 
GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC 
CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT 
AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GCAGGACTCC 
CCAGAAGAGG GCAGAGCACA GCAGGGGGAA CCTGAGGAGG AGGAGGACGA GGACGAGGAG 
GAGGAGGAGG AGGAGGACGA GGGAGGCACA CAGGAACTGG ATGTCGAGGA CTTGCTGGAA 
AACAACTGGA ACATCCTGCA GTTCCTGCCC CAGGCAGCCT CCTGCCAGAG CTACTTCCTC 
ATGATCGTTT CAGCGTACAT CGTGGCCGTG TACCACAGCA TGAAGGAGGA ACTGAGGCGC 
AGCACCCCAG GGAGCACCCC CGTGAGGAGG AGGGGAGCCA GCCAGCTCTC CCAGGAGGCC 
CAGGGGGAGG CCGGAGCCCT TCCTGGAATG ATCACCTTCT CACAAGATTA TGTGGCAAAT 
GAACTCACTC AGAACTTCTT CACCATCACT CAGAAGATTC AGAAGAAAGT CACAGGCTCT 
CGGAACTCTA CTGAGCTCTC AGAAATGTTT CCTGTTCTCC CCGGTTCTCA CCTGCTGCTC 
AATAACCCTG CTCTGGAGTT CATCAAATAC GTGTGCAAGG TCCTGTCTCT GGACACCAAC 
ATCACAAACC AGGTGAACAA GCTAACCCGA GACCTGCTGC GCCTGGTAGA CGTAGGCGAG 
TTCTCAGAGG AGGCCCAGTT CCGAGACCCC TGCCGTTCCT ACGTGCTCCC TGAGGTCATC 
TGCCACAACT GTAACTTCTG CCGGGACCTG GACCTGTGCA AAGACCCCTC CTTCTCTCAG 
GATGGGACCG TGCTGCCTCA GTGGCTCTGC TCCAACTGTC AGGTTGCCTA CGACTCATCA 
GTCATTGAGA TGGCCCTGGT GGAAGCCCTA CAGAAGAAGC TGATGGCCTT TACCCTGCAG 
GACCTGGTCT GCCTGAAGTG CCGGGGTGTG AAGGAGACCA ACATGGCCGT GTACTGCAGC 
TGTGCGGGGG GCTTCTCCCT CACCATCCAC ACTAAGGTCT TCATGGAACA GATCGGAATC 
TTCCAGAACA TCGCCCAGCA CTATGGCATG TCATACCTCA AGGAGACCCT GGATTGGCTG 
CAGCTTCAGA ACCCCCAAGT GGGCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178358.1
CDS	1..6870
Translation	MVLRNTGRLRAEPGADGEPSRDDGSSSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIKLSFNTVEDLVKVRKEISPAVKKNRDQSHTSDAYTTMLSSVLQGGSVIFDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGSAFPVEIARRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEHIQEAKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKSPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEQVINFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCRNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFVLEGKRQVGMAQASEGSQSLGSPDMEDFGLTKPPHSAVPIATKRKRIHCERQEGSQDLELTMPWQEVLGQPPALGTTQEEWLVWLQFHKKKWQLQAQQRLAHKKRRRLEGAEGTPRPGAIREGPSTGLGGFFRRTARSILDLPWQIVQISETSQAGLFRVWAVIGSDLHCIKLNIPRVFYVNQRVAKAEEGPSYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELAAPDIEGVYETQVPLVFRALVQLGCMCVVSKQLVRHLSGWEAETFTLEHLEMRSLAQFNYLEPGSIRHIYLYHHAQGHKALFGIFVPSQRKAAVFVLDTVRSNQMPSLSALYSTEHSLLLEKVGPELLPPQKHAFEVRAETDLKIVCRAIQRFLLTYKEERRGPTLIAVQSNWDLKRLTGEIPVLEEFPLVPVRVADKISYGVLDWQRHGARRMIRHYLNLDTYLSQAFEMSRYFHVPVGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDQAPVEINSSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGADSIGISFDVIQQASLEDMITGNQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYRNVYADNQVMHFYRWLRSPSSLLHDPALHRTLHSMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRVEDALAYVEYITNSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGTVPSRIHCGQQDSPEEGRAQQGEPEEEEDEDEEEEEEEDEGGTQELDVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSTPGSTPVRRRGASQLSQEAQGEAGALPGMITFSQDYVANELTQNFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLTRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICHNCNFCRDLDLCKDPSFSQDGTVLPQWLCSNCQVAYDSSVIEMALVEALQKKLMAFTLQDLVCLKCRGVKETNMAVYCSCAGGFSLTIHTKVFMEQIGIFQNIAQHYGMSYLKETLDWLQLQNPQVGH

Target ORF information:

RefSeq Version	NM_001191429.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191429.1

ORF Insert Sequence:

ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG 
ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT 
GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT 
TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC 
TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG 
GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG 
AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA 
ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC 
ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG 
GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT 
GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA 
CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT 
GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC 
GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG 
GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC 
ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA 
CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC 
CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC 
AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC 
CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG 
TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC 
CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG 
TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG 
GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG 
GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC 
CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT 
GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT 
GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG 
CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA 
GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC 
AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC 
GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG 
CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT 
TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC 
GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG 
TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG 
CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC 
ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG 
GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC 
TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT 
GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG 
CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA 
GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG 
GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG 
GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC 
TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG 
GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG 
GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC 
ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT 
GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT 
TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA 
CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG 
GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA 
CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC 
ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA 
AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC 
CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG 
AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC 
TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC 
TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG 
CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG 
CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA 
CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT 
GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC 
AACCAGATGC CCAGCCTCAG TGCCTTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA 
GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC 
TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT 
GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC 
CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG 
GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC 
ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG 
CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC 
GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC 
TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG 
CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC 
AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG 
GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG 
CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG 
GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC 
CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT 
AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GCAGGACTCC 
CCAGAAGAGG GCAGAGCACA GCAGGGGGAA CCTGAGGAGG AGGAGGACGA GGACGAGGAG 
GAGGAGGAGG AGGAGGACGA GGGAGGCACA CAGGAACTGG ATGTCGAGGA CTTGCTGGAA 
AACAACTGGA ACATCCTGCA GTTCCTGCCC CAGGCAGCCT CCTGCCAGAG CTACTTCCTC 
ATGATCGTTT CAGCGTACAT CGTGGCCGTG TACCACAGCA TGAAGGAGGA ACTGAGGCGC 
AGCACCCCAG GGAGCACCCC CGTGAGGAGG AGGGGAGCCA GCCAGCTCTC CCAGGAGGCC 
CAGGGGGAGG CCGGAGCCCT TCCTGGAATG ATCACCTTCT CACAAGATTA TGTGGCAAAT 
GAACTCACTC AGAACTTCTT CACCATCACT CAGAAGATTC AGAAGAAAGT CACAGGCTCT 
CGGAACTCTA CTGAGCTCTC AGAAATGTTT CCTGTTCTCC CCGGTTCTCA CCTGCTGCTC 
AATAACCCTG CTCTGGAGTT CATCAAATAC GTGTGCAAGG TCCTGTCTCT GGACACCAAC 
ATCACAAACC AGGTGAACAA GCTAACCCGA GACCTGCTGC GCCTGGTAGA CGTAGGCGAG 
TTCTCAGAGG AGGCCCAGTT CCGAGACCCC TGCCGTTCCT ACGTGCTCCC TGAGGTCATC 
TGCCACAACT GTAACTTCTG CCGGGACCTG GACCTGTGCA AAGACCCCTC CTTCTCTCAG 
GATGGGACCG TGCTGCCTCA GTGGCTCTGC TCCAACTGTC AGGTTGCCTA CGACTCATCA 
GTCATTGAGA TGGCCCTGGT GGAAGCCCTA CAGAAGAAGC TGATGGCCTT TACCCTGCAG 
GACCTGGTCT GCCTGAAGTG CCGGGGTGTG AAGGAGACCA ACATGGCCGT GTACTGCAGC 
TGTGCGGGGG GCTTCTCCCT CACCATCCAC ACTAAGGTCT TCATGGAACA GATCGGAATC 
TTCCAGAACA TCGCCCAGCA CTATGGCATG TCATACCTCA AGGAGACCCT GGATTGGCTG 
CAGCTTCAGA ACCCCCAAGT GGGCCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_005217684.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217741.1 Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

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CloneID	OBa224428
Clone ID Related Accession (Same CDS sequence)	XM_005217684.4
Accession Version	XM_005217684.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	DNA polymerase epsilon catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217684.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG 
ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT 
GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT 
TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC 
TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG 
GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG 
AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA 
ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC 
ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG 
GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT 
GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA 
CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT 
GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC 
GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG 
GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC 
ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA 
CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC 
CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC 
AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC 
CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG 
TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC 
CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG 
TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG 
GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG 
GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC 
CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT 
GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT 
GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG 
CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA 
GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC 
AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC 
GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG 
CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT 
TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC 
GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG 
TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG 
CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC 
ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG 
GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC 
TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT 
GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG 
CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA 
GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG 
GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG 
GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC 
TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG 
GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG 
GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC 
ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT 
GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT 
TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA 
CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG 
GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA 
CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC 
ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA 
AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC 
CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG 
AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC 
TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC 
TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG 
CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG 
CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA 
CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT 
GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC 
AACCAGATGC CCAGCCTCAG TGCCCTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA 
GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC 
TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT 
GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC 
CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG 
GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC 
ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG 
CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC 
GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC 
TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG 
CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC 
AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG 
GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG 
CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG 
GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC 
CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT 
AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GGACTCCCCA 
GAAGAGGGCA GAGCACAGCA GGGGGAACCT GAGGAGGAGG AGGACGAGGA CGAGGAGGAG 
GAGGAGGAGG AGGACGAGGG AGGCACACAG GAACTGGATG TCGAGGACTT GCTGGAAAAC 
AACTGGAACA TCCTGCAGTT CCTGCCCCAG GCAGCCTCCT GCCAGAGCTA CTTCCTCATG 
ATCGTTTCAG CGTACATCGT GGCCGTGTAC CACAGCATGA AGGAGGAACT GAGGCGCAGC 
ACCCCAGGGA GCACCCCCGT GAGGAGGAGG GGAGCCAGCC AGCTCTCCCA GGAGGCCCAG 
GGGGAGGCCG GAGCCCTTCC TGGAATGATC ACCTTCTCAC AAGATTATGT GGCAAATGAA 
CTCACTCAGA ACTTCTTCAC CATCACTCAG AAGATTCAGA AGAAAGTCAC AGGCTCTCGG 
AACTCTACTG AGCTCTCAGA AATGTTTCCT GTTCTCCCCG GTTCTCACCT GCTGCTCAAT 
AACCCTGCTC TGGAGTTCAT CAAATACGTG TGCAAGGTCC TGTCTCTGGA CACCAACATC 
ACAAACCAGG TGAACAAGCT AACCCGAGAC CTGCTGCGCC TGGTAGACGT AGGCGAGTTC 
TCAGAGGAGG CCCAGTTCCG AGACCCCTGC CGTTCCTACG TGCTCCCTGA GGTCATCTGC 
CACAACTGTA ACTTCTGCCG GGACCTGGAC CTGTGCAAAG ACCCCTCCTT CTCTCAGGAT 
GGGACCGTGC TGCCTCAGTG GCTCTGCTCC AACTGTCAGG TTGCCTACGA CTCATCAGTC 
ATTGAGATGG CCCTGGTGGA AGCCCTACAG AAGAAGCTGA TGGCCTTTAC CCTGCAGGAC 
CTGGTCTGCC TGAAGTGCCG GGGTGTGAAG GAGACCAACA TGGCCGTGTA CTGCAGCTGT 
GCGGGGGGCT TCTCCCTCAC CATCCACACT AAGGTCTTCA TGGAACAGAT CGGAATCTTC 
CAGAACATCG CCCAGCACTA TGGCATGTCA TACCTCAAGG AGACCCTGGA TTGGCTGCAG 
CTTCAGAACC CCCAAGTGGG CCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217741.1
CDS	80..6946
Translation	MVLRNTGRLRAEPGADGEPSRDDGSSSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIKLSFNTVEDLVKVRKEISPAVKKNRDQSHTSDAYTTMLSSVLQGGSVIFDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGSAFPVEIARRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEHIQEAKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKSPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEQVINFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCRNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFVLEGKRQVGMAQASEGSQSLGSPDMEDFGLTKPPHSAVPIATKRKRIHCERQEGSQDLELTMPWQEVLGQPPALGTTQEEWLVWLQFHKKKWQLQAQQRLAHKKRRRLEGAEGTPRPGAIREGPSTGLGGFFRRTARSILDLPWQIVQISETSQAGLFRVWAVIGSDLHCIKLNIPRVFYVNQRVAKAEEGPSYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELAAPDIEGVYETQVPLVFRALVQLGCMCVVSKQLVRHLSGWEAETFTLEHLEMRSLAQFNYLEPGSIRHIYLYHHAQGHKALFGIFVPSQRKAAVFVLDTVRSNQMPSLSALYSTEHSLLLEKVGPELLPPQKHAFEVRAETDLKIVCRAIQRFLLTYKEERRGPTLIAVQSNWDLKRLTGEIPVLEEFPLVPVRVADKISYGVLDWQRHGARRMIRHYLNLDTYLSQAFEMSRYFHVPVGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDQAPVEINSSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGADSIGISFDVIQQASLEDMITGNQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYRNVYADNQVMHFYRWLRSPSSLLHDPALHRTLHSMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRVEDALAYVEYITNSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGTVPSRIHCGQDSPEEGRAQQGEPEEEEDEDEEEEEEEDEGGTQELDVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSTPGSTPVRRRGASQLSQEAQGEAGALPGMITFSQDYVANELTQNFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLTRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICHNCNFCRDLDLCKDPSFSQDGTVLPQWLCSNCQVAYDSSVIEMALVEALQKKLMAFTLQDLVCLKCRGVKETNMAVYCSCAGGFSLTIHTKVFMEQIGIFQNIAQHYGMSYLKETLDWLQLQNPQVGH

Target ORF information:

RefSeq Version	XM_005217684.4
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217684.4

ORF Insert Sequence:

ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG 
ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT 
GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT 
TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC 
TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG 
GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG 
AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA 
ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC 
ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG 
GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT 
GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA 
CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT 
GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC 
GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG 
GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC 
ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA 
CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC 
CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC 
AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC 
CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG 
TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC 
CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG 
TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG 
GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG 
GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC 
CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT 
GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT 
GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG 
CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA 
GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC 
AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC 
GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG 
CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT 
TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC 
GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG 
TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG 
CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC 
ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG 
GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC 
TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT 
GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG 
CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA 
GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG 
GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG 
GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC 
TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG 
GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG 
GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC 
ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT 
GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT 
TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA 
CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG 
GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA 
CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC 
ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA 
AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC 
CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG 
AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC 
TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC 
TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG 
CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG 
CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA 
CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT 
GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC 
AACCAGATGC CCAGCCTCAG TGCCCTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA 
GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC 
TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT 
GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC 
CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG 
GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC 
ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG 
CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC 
GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC 
TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG 
CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC 
AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG 
GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG 
CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG 
GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC 
CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT 
AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GGACTCCCCA 
GAAGAGGGCA GAGCACAGCA GGGGGAACCT GAGGAGGAGG AGGACGAGGA CGAGGAGGAG 
GAGGAGGAGG AGGACGAGGG AGGCACACAG GAACTGGATG TCGAGGACTT GCTGGAAAAC 
AACTGGAACA TCCTGCAGTT CCTGCCCCAG GCAGCCTCCT GCCAGAGCTA CTTCCTCATG 
ATCGTTTCAG CGTACATCGT GGCCGTGTAC CACAGCATGA AGGAGGAACT GAGGCGCAGC 
ACCCCAGGGA GCACCCCCGT GAGGAGGAGG GGAGCCAGCC AGCTCTCCCA GGAGGCCCAG 
GGGGAGGCCG GAGCCCTTCC TGGAATGATC ACCTTCTCAC AAGATTATGT GGCAAATGAA 
CTCACTCAGA ACTTCTTCAC CATCACTCAG AAGATTCAGA AGAAAGTCAC AGGCTCTCGG 
AACTCTACTG AGCTCTCAGA AATGTTTCCT GTTCTCCCCG GTTCTCACCT GCTGCTCAAT 
AACCCTGCTC TGGAGTTCAT CAAATACGTG TGCAAGGTCC TGTCTCTGGA CACCAACATC 
ACAAACCAGG TGAACAAGCT AACCCGAGAC CTGCTGCGCC TGGTAGACGT AGGCGAGTTC 
TCAGAGGAGG CCCAGTTCCG AGACCCCTGC CGTTCCTACG TGCTCCCTGA GGTCATCTGC 
CACAACTGTA ACTTCTGCCG GGACCTGGAC CTGTGCAAAG ACCCCTCCTT CTCTCAGGAT 
GGGACCGTGC TGCCTCAGTG GCTCTGCTCC AACTGTCAGG TTGCCTACGA CTCATCAGTC 
ATTGAGATGG CCCTGGTGGA AGCCCTACAG AAGAAGCTGA TGGCCTTTAC CCTGCAGGAC 
CTGGTCTGCC TGAAGTGCCG GGGTGTGAAG GAGACCAACA TGGCCGTGTA CTGCAGCTGT 
GCGGGGGGCT TCTCCCTCAC CATCCACACT AAGGTCTTCA TGGAACAGAT CGGAATCTTC 
CAGAACATCG CCCAGCACTA TGGCATGTCA TACCTCAAGG AGACCCTGGA TTGGCTGCAG 
CTTCAGAACC CCCAAGTGGG CCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_024977498.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833266.1 Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

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CloneID	OBa224429
Clone ID Related Accession (Same CDS sequence)	XM_024977498.1
Accession Version	XM_024977498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	DNA polymerase epsilon catalytic subunit A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTGC GGTTTGGTTT TGAGAGACTC AAGGAGCCTG GTGAGAAGAC AGGCTGGCTC 
ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGATAAAC GCTTAGTTAG TGCTGTGGAT 
TACTACTTTA TTCAAGACGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CAAGCCATAT 
TTCTACATTG CGACCAGAAA GGGTTGTGAG CGAGAAGTTT CATCTTTTCT TTCTAAGAAG 
TTTCAGGGTA AAATTGCCAA AGTAGAGACT GTCCCTAAGG AGGATCTGGA CTTGCCAAAT 
CACTTGGTTG GTTTGAAACG AAATTACATT AAGCTGTCCT TCAACACTGT GGAGGACCTC 
GTCAAAGTGA GGAAGGAGAT CTCCCCAGCT GTGAAGAAAA ACCGGGACCA GAGTCATACC 
AGTGATGCCT ACACAACTAT GCTCTCCAGT GTTCTGCAAG GGGGCAGTGT AATTTTTGAT 
GAAGAGGAAA CCTCTAAGAA GATTGCCGAC CAGTTGGACA ACATTGTGGA CATGCGAGAG 
TATGATGTAC CTTACCACAT CCGTCTCTCC ATCGACCTGA AGATCCACGT GGCTCACTGG 
TACAATGTCC GGTACCGAGG AAGCGCCTTT CCAGTGGAAA TTGCCCGCCG TGATGACCTC 
GTTGAACGAC CAGACCCCGT GGTTTTAGCA TTTGACATTG AGACAACCAA ACTGCCCCTC 
AAGTTTCCAG ATGCTGAGAC CGACCAGATT ATGATGATTT CCTATATGAT TGATGGTCAG 
GGCTACCTCA TCACCAACAG GGAGATTGTC TCAGAAGATA TAGAAGATTT CGAATTTACC 
CCCAAGCCAG AATATGAAGG ACCCTTCTGT GTCTTCAATG AGCCCGATGA GGTTCATCTA 
ATCCAAAGAT GGTTTGAACA CATCCAGGAG GCCAAACCCA CCATCATGGT CACCTATAAT 
GGGGACTTTT TTGACTGGCC ATTTGTGGAG GCCAGAGCAG CGGTCCATGG ACTGAGCATG 
TACCAGGAGA TAGGGTTCCA GAAGGACAGC CAGGGGGAGT ACAAATCACC CCAGTGTATC 
CACATGGACT GTCTCAGGTG GGTGAAAAGG GACAGTTACC TGCCCGTGGG CAGCCACAAC 
CTCAAGGCGG CGGCCAAAGC CAAGCTGGGC TACGACCCAG TGGAACTGGA CCCTGAAGAC 
ATGTGCCGGA TGGCCACTGA GCAGCCCCAG ACTCTGGCGA CCTACTCGGT GTCGGATGCA 
GTGGCCACAT ACTACCTGTA CATGAAGTAT GTCCACCCGT TTATATTCGC CCTGTGTACC 
ATCATCCCCA TGGAGCCCGA TGAGGTGCTG CGGAAAGGTT CTGGGACGCT GTGCGAGGCC 
TTGCTAATGG TGCAGGCCTT CCATGCCAAC ATCATCTTCC CCAACAAGCA GGAGCAGGAG 
TTCAACAAGC TGACAGATGA CGGCCACGTG CTGGATGCTG AGACCTACGT GGGTGGCCAC 
GTAGAAGCCC TGGAGTCCGG CGTGTTCCGC AGTGACATCC CCTGCCGGTT CCGGATGAAT 
CCTGCTGCCT TTGACTTCCT GCTGCAGCGG GTGGAGAAAA CCATGCGTCA TGCCATCGAG 
GAAGAGGAGA AGGTGCCCAT GGAGCAGGTC ATCAACTTCC AAGAGGTGTG TGATCAGATT 
AAGACCAAGC TCACCTCCCT GAAAGATGTT CCTAACCGAA TTGAGTGTCC CCTCATCTAC 
CACCTGGACG TGGGGGCCAT GTACCCCAAC ATCATCTTGA CGAACCGCCT GCAGCCCTCT 
GCCATGGTGG ATGAGGCCAC CTGCGCAGCC TGTGACTTCA ACAAGCCTGG AGCGAACTGC 
CAGAGGAAGA TGGCCTGGCA GTGGAGGGGA GAGTTCATGC CAGCCAGTCG CAGTGAGTAT 
CATCGGATCC AGCACCAACT GGAGTCAGAG AAGTTCCCTC CCTTGACCCC CGAGGGCCCA 
GCCCGGGCCT TTCACGAGCT GTCCCGTGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG 
GCAGATTACT GCCGGAAGGC ATACAAGAAA ATCCACGTGA CCAAGGTGGA AGAGCGTCTC 
ACCACCATCT GCCAGCGGGA GAACTCCTTC TATGTGGACA CTGTACGTGC TTTTCGGGAC 
AGGCGCTATG AGTTCAAAGG TCTCCACAAG GTGTGGAAAA AGAAGCTCTC CGCAGCCGTG 
GAGGTGGGGG ACGCGGCGGA GGTGAAGCGC TGCAGGAACA TGGAGGTCCT GTACGACTCC 
CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAAGGGG 
GCCCGCTGGT ACTCCATGGA GATGGCTGGC ATCGTCTGCT TCACAGGGGC CAACATCATC 
ACCCAGGCAC GGGAGCTGAT CGAGCAAATC GGGAGGCCCT TAGAGCTGGA CACAGATGGA 
ATATGGTGTG TCCTGCCCAA CAGCTTCCCC GAAAACTTTG TCATCAAGAC CACCAATGTG 
AAAAAGCCCA AGGTGACCAT CTCTTACCCT GGGGCTATGT TAAACATCAT GGTCAAGGAA 
GGCTTCACCA ATGATCAGTA CCAGGAGCTG GCTGAGCCTT CTTCACTAAC CTATGTCACT 
CGCTCAGAGA ACAGCATCTT TTTCGAGGTT GATGGGCCCT ACCTTGCCAT GATCCTTCCA 
GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGGTATG CCGTGTTCAA TGAGGATGGT 
TCCCTGGCTG AGCTCAAGGG CTTTGAGGTC AAACGCCGTG GGGAGCTGCA GCTGATTAAA 
ATCTTCCAGT CCTCAGTGTT TGAGGCCTTT CTTAAGGGCA GCACCCTAGA AGAAGTGTAT 
GGCTCTGTCG CCAAGGTGGC TGATTACTGG CTGGACGTAC TATACAGCAA GGCAGCTAAC 
ATGCCTGATT CCGAACTGTT CGAGCTGATC TCCGAGAACC GTTCCATGTC TCGGAAGCTG 
GAAGATTATG GGGAGCAGAA GTCGACATCC ATCAGCACGG CCAAGCGCCT GGCTGAGTTC 
CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GCTACATCAT CTCCCGGAAG 
CCTGAGGGGT CTCCAGTCAC AGAGAGAGCC ATTCCACTGG CCATTTTCCA GGCAGAGCCC 
ACCGTGAGGA AGCACTTTCT CCGAAAATGG CTAAAGAGTT CTTCCCTTCA GGACTTTGAT 
ATTCGGACAA TTCTGGATTG GGACTACTAC ATTGAGCGGC TGGGGAGTGC CATACAGAAG 
ATCATCACGA TCCCTGCGGC CCTGCAGCAG GTGAAGAACC CAGTACCGCG TGTCAAACAC 
CCTGACTGGC TGCACAAAAA ACTGTTAGAG AAAAATGACG TCTACAAGCA GAAGAAGATC 
AGTGAGCTCT TCGTCCTTGA GGGCAAGAGA CAGGTGGGCA TGGCCCAAGC TTCAGAAGGT 
TCCCAAAGCC TTGGCTCCCC CGACATGGAG GACTTTGGCC TCACAAAGCC ACCTCACTCG 
GCAGTGCCCA TTGCTACCAA GAGAAAGCGT ATCCACTGTG AGAGGCAAGA GGGATCGCAG 
GACCTGGAGC TGACGATGCC CTGGCAGGAG GTTTTGGGGC AGCCTCCGGC ACTCGGAACC 
ACCCAGGAAG AGTGGCTGGT CTGGCTCCAA TTCCACAAGA AGAAGTGGCA GCTGCAGGCT 
CAACAGCGCC TTGCTCACAA GAAGAGGCGG CGTCTGGAGG GGGCAGAGGG CACACCAAGG 
CCTGGGGCCA TCCGAGAGGG GCCCTCCACA GGACTGGGGG GCTTCTTTCG AAGAACAGCT 
CGCAGCATTC TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAGACCAG CCAGGCTGGC 
TTGTTCAGGG TATGGGCTGT CATCGGCAGT GACCTGCACT GCATCAAGCT GAACATTCCC 
CGGGTGTTCT ACGTGAACCA GCGGGTGGCC AAAGCGGAGG AGGGCCCTTC CTATCGGAAG 
GTGAATCGAG TCCTTCCTCG CTCCAATATG GTCTACAACC TCTACGAATA CTCGGTGCCA 
GAGGACATGT ACCAAGAACA TATCAACGAG ATCAACACTG AGCTGGCGGC GCCAGACATC 
GAGGGCGTGT ATGAGACTCA GGTTCCATTA GTGTTTCGGG CCCTGGTGCA GCTGGGCTGC 
ATGTGTGTGG TCAGTAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTC 
ACTCTTGAGC ACCTGGAGAT GCGTTCCTTG GCCCAGTTCA ACTACCTGGA ACCAGGCAGC 
ATCCGCCACA TCTACCTGTA CCACCACGCA CAGGGCCACA AGGCTCTCTT TGGCATCTTC 
GTACCCTCAC AGCGCAAGGC AGCTGTGTTT GTGCTGGATA CGGTGCGAAG CAACCAGATG 
CCCAGCCTCA GTGCCCTGTA CTCAACCGAG CACAGCCTCC TGCTGGAGAA AGTGGGCCCT 
GAGCTTCTGC CCCCACAGAA ACACGCTTTT GAAGTTCGTG CTGAAACAGA CTTGAAGATC 
GTCTGCAGAG CCATCCAGCG CTTCCTGTTG ACCTACAAAG AGGAGCGCCG TGGGCCAACT 
CTCATTGCTG TTCAGTCCAA CTGGGACCTG AAGAGGCTGA CTGGCGAGAT CCCTGTCCTG 
GAGGAATTCC CACTGGTGCC TGTCCGCGTG GCTGACAAGA TCAGCTACGG GGTCCTGGAC 
TGGCAGCGCC ACGGAGCCCG GCGCATGATC CGGCACTACC TCAACCTGGA CACGTATCTG 
TCACAGGCCT TCGAGATGAG CAGGTACTTC CACGTCCCCG TTGGGAATCT GCCTGAGGAC 
ATCTCTACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCACC TCCAGCGCCA CAACCACCTG 
CTCTGGCTCT CTCCTACGTC TCGCCCAGAC CTGGGTGGGA AGGAGGCCGA CGACAACAGG 
CTCGTCATGG AGTTTGATGA CCAAGCCCCT GTGGAGATCA ACAGTTCCGG CTGTTACTCC 
ACAGTGTGCG TGGAACTGGA TATTCAGAAC CTGGCCGTCA ACACCATCCT GCAGTCTCAC 
CACGTCAATG ATATGGAGGG GGCCGACAGC ATTGGCATCA GCTTCGATGT GATCCAGCAG 
GCCTCCCTGG AGGACATGAT CACAGGCAAT CAGGCTGCCA GCGCCCCTGC CAGCTATGAC 
GAGACAGCCC TCTGCTCCAA CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG 
AAGGAGATCA CGCAGTACCG CAACGTCTAC GCCGACAACC AGGTGATGCA CTTCTACCGC 
TGGCTCCGGT CGCCGTCCTC GCTGCTGCAC GACCCTGCCC TGCACCGCAC GCTGCACAGC 
ATGATGAAGA AGCTCTTCCT TCAACTCATC GCTGAGTTCA AGCGCCTGGG GTCATCGGTC 
GTCTATGCCA ACTTCAACCG CATCATCCTC TGCACCAAGA AGCGGCGGGT GGAGGACGCC 
CTGGCCTACG TGGAGTACAT CACCAACAGC ATCCACTCTA AAGAGATCTT CCATTCTTTG 
ACAATTTCCT TTTCTCGATG CTGGGAATTT CTTCTCTGGA TGGATCCCTC TAATTATGGT 
GGAATCAAAG GAACTGTTCC ATCTAGGATT CACTGTGGAC AGCAGGACTC CCCAGAAGAG 
GGCAGAGCAC AGCAGGGGGA ACCTGAGGAG GAGGAGGACG AGGACGAGGA GGAGGAGGAG 
GAGGAGGACG AGGGAGGCAC ACAGGAACTG GATGTCGAGG ACTTGCTGGA AAACAACTGG 
AACATCCTGC AGTTCCTGCC CCAGGCAGCC TCCTGCCAGA GCTACTTCCT CATGATCGTT 
TCAGCGTACA TCGTGGCCGT GTACCACAGC ATGAAGGAGG AACTGAGGCG CAGCACCCCA 
GGGAGCACCC CCGTGAGGAG GAGGGGAGCC AGCCAGCTCT CCCAGGAGGC CCAGGGGGAG 
GCCGGAGCCC TTCCTGGAAT GATCACCTTC TCACAAGATT ATGTGGCAAA TGAACTCACT 
CAGAACTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCT 
ACTGAGCTCT CAGAAATGTT TCCTGTTCTC CCCGGTTCTC ACCTGCTGCT CAATAACCCT 
GCTCTGGAGT TCATCAAATA CGTGTGCAAG GTCCTGTCTC TGGACACCAA CATCACAAAC 
CAGGTGAACA AGCTAACCCG AGACCTGCTG CGCCTGGTAG ACGTAGGCGA GTTCTCAGAG 
GAGGCCCAGT TCCGAGACCC CTGCCGTTCC TACGTGCTCC CTGAGGTCAT CTGCCACAAC 
TGTAACTTCT GCCGGGACCT GGACCTGTGC AAAGACCCCT CCTTCTCTCA GGATGGGACC 
GTGCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGTTGCCT ACGACTCATC AGTCATTGAG 
ATGGCCCTGG TGGAAGCCCT ACAGAAGAAG CTGATGGCCT TTACCCTGCA GGACCTGGTC 
TGCCTGAAGT GCCGGGGTGT GAAGGAGACC AACATGGCCG TGTACTGCAG CTGTGCGGGG 
GGCTTCTCCC TCACCATCCA CACTAAGGTC TTCATGGAAC AGATCGGAAT CTTCCAGAAC 
ATCGCCCAGC ACTATGGCAT GTCATACCTC AAGGAGACCC TGGATTGGCT GCAGCTTCAG 
AACCCCCAAG TGGGCCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833266.1
CDS	153..6893
Translation	MDLRFGFERLKEPGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIKLSFNTVEDLVKVRKEISPAVKKNRDQSHTSDAYTTMLSSVLQGGSVIFDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGSAFPVEIARRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEHIQEAKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKSPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEQVINFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCRNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFVLEGKRQVGMAQASEGSQSLGSPDMEDFGLTKPPHSAVPIATKRKRIHCERQEGSQDLELTMPWQEVLGQPPALGTTQEEWLVWLQFHKKKWQLQAQQRLAHKKRRRLEGAEGTPRPGAIREGPSTGLGGFFRRTARSILDLPWQIVQISETSQAGLFRVWAVIGSDLHCIKLNIPRVFYVNQRVAKAEEGPSYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELAAPDIEGVYETQVPLVFRALVQLGCMCVVSKQLVRHLSGWEAETFTLEHLEMRSLAQFNYLEPGSIRHIYLYHHAQGHKALFGIFVPSQRKAAVFVLDTVRSNQMPSLSALYSTEHSLLLEKVGPELLPPQKHAFEVRAETDLKIVCRAIQRFLLTYKEERRGPTLIAVQSNWDLKRLTGEIPVLEEFPLVPVRVADKISYGVLDWQRHGARRMIRHYLNLDTYLSQAFEMSRYFHVPVGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDQAPVEINSSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGADSIGISFDVIQQASLEDMITGNQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYRNVYADNQVMHFYRWLRSPSSLLHDPALHRTLHSMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRVEDALAYVEYITNSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGTVPSRIHCGQQDSPEEGRAQQGEPEEEEDEDEEEEEEEDEGGTQELDVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSTPGSTPVRRRGASQLSQEAQGEAGALPGMITFSQDYVANELTQNFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLTRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICHNCNFCRDLDLCKDPSFSQDGTVLPQWLCSNCQVAYDSSVIEMALVEALQKKLMAFTLQDLVCLKCRGVKETNMAVYCSCAGGFSLTIHTKVFMEQIGIFQNIAQHYGMSYLKETLDWLQLQNPQVGH

Target ORF information:

RefSeq Version	XM_024977498.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977498.1

ORF Insert Sequence:

ATGGATCTGC GGTTTGGTTT TGAGAGACTC AAGGAGCCTG GTGAGAAGAC AGGCTGGCTC 
ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGATAAAC GCTTAGTTAG TGCTGTGGAT 
TACTACTTTA TTCAAGACGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CAAGCCATAT 
TTCTACATTG CGACCAGAAA GGGTTGTGAG CGAGAAGTTT CATCTTTTCT TTCTAAGAAG 
TTTCAGGGTA AAATTGCCAA AGTAGAGACT GTCCCTAAGG AGGATCTGGA CTTGCCAAAT 
CACTTGGTTG GTTTGAAACG AAATTACATT AAGCTGTCCT TCAACACTGT GGAGGACCTC 
GTCAAAGTGA GGAAGGAGAT CTCCCCAGCT GTGAAGAAAA ACCGGGACCA GAGTCATACC 
AGTGATGCCT ACACAACTAT GCTCTCCAGT GTTCTGCAAG GGGGCAGTGT AATTTTTGAT 
GAAGAGGAAA CCTCTAAGAA GATTGCCGAC CAGTTGGACA ACATTGTGGA CATGCGAGAG 
TATGATGTAC CTTACCACAT CCGTCTCTCC ATCGACCTGA AGATCCACGT GGCTCACTGG 
TACAATGTCC GGTACCGAGG AAGCGCCTTT CCAGTGGAAA TTGCCCGCCG TGATGACCTC 
GTTGAACGAC CAGACCCCGT GGTTTTAGCA TTTGACATTG AGACAACCAA ACTGCCCCTC 
AAGTTTCCAG ATGCTGAGAC CGACCAGATT ATGATGATTT CCTATATGAT TGATGGTCAG 
GGCTACCTCA TCACCAACAG GGAGATTGTC TCAGAAGATA TAGAAGATTT CGAATTTACC 
CCCAAGCCAG AATATGAAGG ACCCTTCTGT GTCTTCAATG AGCCCGATGA GGTTCATCTA 
ATCCAAAGAT GGTTTGAACA CATCCAGGAG GCCAAACCCA CCATCATGGT CACCTATAAT 
GGGGACTTTT TTGACTGGCC ATTTGTGGAG GCCAGAGCAG CGGTCCATGG ACTGAGCATG 
TACCAGGAGA TAGGGTTCCA GAAGGACAGC CAGGGGGAGT ACAAATCACC CCAGTGTATC 
CACATGGACT GTCTCAGGTG GGTGAAAAGG GACAGTTACC TGCCCGTGGG CAGCCACAAC 
CTCAAGGCGG CGGCCAAAGC CAAGCTGGGC TACGACCCAG TGGAACTGGA CCCTGAAGAC 
ATGTGCCGGA TGGCCACTGA GCAGCCCCAG ACTCTGGCGA CCTACTCGGT GTCGGATGCA 
GTGGCCACAT ACTACCTGTA CATGAAGTAT GTCCACCCGT TTATATTCGC CCTGTGTACC 
ATCATCCCCA TGGAGCCCGA TGAGGTGCTG CGGAAAGGTT CTGGGACGCT GTGCGAGGCC 
TTGCTAATGG TGCAGGCCTT CCATGCCAAC ATCATCTTCC CCAACAAGCA GGAGCAGGAG 
TTCAACAAGC TGACAGATGA CGGCCACGTG CTGGATGCTG AGACCTACGT GGGTGGCCAC 
GTAGAAGCCC TGGAGTCCGG CGTGTTCCGC AGTGACATCC CCTGCCGGTT CCGGATGAAT 
CCTGCTGCCT TTGACTTCCT GCTGCAGCGG GTGGAGAAAA CCATGCGTCA TGCCATCGAG 
GAAGAGGAGA AGGTGCCCAT GGAGCAGGTC ATCAACTTCC AAGAGGTGTG TGATCAGATT 
AAGACCAAGC TCACCTCCCT GAAAGATGTT CCTAACCGAA TTGAGTGTCC CCTCATCTAC 
CACCTGGACG TGGGGGCCAT GTACCCCAAC ATCATCTTGA CGAACCGCCT GCAGCCCTCT 
GCCATGGTGG ATGAGGCCAC CTGCGCAGCC TGTGACTTCA ACAAGCCTGG AGCGAACTGC 
CAGAGGAAGA TGGCCTGGCA GTGGAGGGGA GAGTTCATGC CAGCCAGTCG CAGTGAGTAT 
CATCGGATCC AGCACCAACT GGAGTCAGAG AAGTTCCCTC CCTTGACCCC CGAGGGCCCA 
GCCCGGGCCT TTCACGAGCT GTCCCGTGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG 
GCAGATTACT GCCGGAAGGC ATACAAGAAA ATCCACGTGA CCAAGGTGGA AGAGCGTCTC 
ACCACCATCT GCCAGCGGGA GAACTCCTTC TATGTGGACA CTGTACGTGC TTTTCGGGAC 
AGGCGCTATG AGTTCAAAGG TCTCCACAAG GTGTGGAAAA AGAAGCTCTC CGCAGCCGTG 
GAGGTGGGGG ACGCGGCGGA GGTGAAGCGC TGCAGGAACA TGGAGGTCCT GTACGACTCC 
CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAAGGGG 
GCCCGCTGGT ACTCCATGGA GATGGCTGGC ATCGTCTGCT TCACAGGGGC CAACATCATC 
ACCCAGGCAC GGGAGCTGAT CGAGCAAATC GGGAGGCCCT TAGAGCTGGA CACAGATGGA 
ATATGGTGTG TCCTGCCCAA CAGCTTCCCC GAAAACTTTG TCATCAAGAC CACCAATGTG 
AAAAAGCCCA AGGTGACCAT CTCTTACCCT GGGGCTATGT TAAACATCAT GGTCAAGGAA 
GGCTTCACCA ATGATCAGTA CCAGGAGCTG GCTGAGCCTT CTTCACTAAC CTATGTCACT 
CGCTCAGAGA ACAGCATCTT TTTCGAGGTT GATGGGCCCT ACCTTGCCAT GATCCTTCCA 
GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGGTATG CCGTGTTCAA TGAGGATGGT 
TCCCTGGCTG AGCTCAAGGG CTTTGAGGTC AAACGCCGTG GGGAGCTGCA GCTGATTAAA 
ATCTTCCAGT CCTCAGTGTT TGAGGCCTTT CTTAAGGGCA GCACCCTAGA AGAAGTGTAT 
GGCTCTGTCG CCAAGGTGGC TGATTACTGG CTGGACGTAC TATACAGCAA GGCAGCTAAC 
ATGCCTGATT CCGAACTGTT CGAGCTGATC TCCGAGAACC GTTCCATGTC TCGGAAGCTG 
GAAGATTATG GGGAGCAGAA GTCGACATCC ATCAGCACGG CCAAGCGCCT GGCTGAGTTC 
CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GCTACATCAT CTCCCGGAAG 
CCTGAGGGGT CTCCAGTCAC AGAGAGAGCC ATTCCACTGG CCATTTTCCA GGCAGAGCCC 
ACCGTGAGGA AGCACTTTCT CCGAAAATGG CTAAAGAGTT CTTCCCTTCA GGACTTTGAT 
ATTCGGACAA TTCTGGATTG GGACTACTAC ATTGAGCGGC TGGGGAGTGC CATACAGAAG 
ATCATCACGA TCCCTGCGGC CCTGCAGCAG GTGAAGAACC CAGTACCGCG TGTCAAACAC 
CCTGACTGGC TGCACAAAAA ACTGTTAGAG AAAAATGACG TCTACAAGCA GAAGAAGATC 
AGTGAGCTCT TCGTCCTTGA GGGCAAGAGA CAGGTGGGCA TGGCCCAAGC TTCAGAAGGT 
TCCCAAAGCC TTGGCTCCCC CGACATGGAG GACTTTGGCC TCACAAAGCC ACCTCACTCG 
GCAGTGCCCA TTGCTACCAA GAGAAAGCGT ATCCACTGTG AGAGGCAAGA GGGATCGCAG 
GACCTGGAGC TGACGATGCC CTGGCAGGAG GTTTTGGGGC AGCCTCCGGC ACTCGGAACC 
ACCCAGGAAG AGTGGCTGGT CTGGCTCCAA TTCCACAAGA AGAAGTGGCA GCTGCAGGCT 
CAACAGCGCC TTGCTCACAA GAAGAGGCGG CGTCTGGAGG GGGCAGAGGG CACACCAAGG 
CCTGGGGCCA TCCGAGAGGG GCCCTCCACA GGACTGGGGG GCTTCTTTCG AAGAACAGCT 
CGCAGCATTC TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAGACCAG CCAGGCTGGC 
TTGTTCAGGG TATGGGCTGT CATCGGCAGT GACCTGCACT GCATCAAGCT GAACATTCCC 
CGGGTGTTCT ACGTGAACCA GCGGGTGGCC AAAGCGGAGG AGGGCCCTTC CTATCGGAAG 
GTGAATCGAG TCCTTCCTCG CTCCAATATG GTCTACAACC TCTACGAATA CTCGGTGCCA 
GAGGACATGT ACCAAGAACA TATCAACGAG ATCAACACTG AGCTGGCGGC GCCAGACATC 
GAGGGCGTGT ATGAGACTCA GGTTCCATTA GTGTTTCGGG CCCTGGTGCA GCTGGGCTGC 
ATGTGTGTGG TCAGTAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTC 
ACTCTTGAGC ACCTGGAGAT GCGTTCCTTG GCCCAGTTCA ACTACCTGGA ACCAGGCAGC 
ATCCGCCACA TCTACCTGTA CCACCACGCA CAGGGCCACA AGGCTCTCTT TGGCATCTTC 
GTACCCTCAC AGCGCAAGGC AGCTGTGTTT GTGCTGGATA CGGTGCGAAG CAACCAGATG 
CCCAGCCTCA GTGCCCTGTA CTCAACCGAG CACAGCCTCC TGCTGGAGAA AGTGGGCCCT 
GAGCTTCTGC CCCCACAGAA ACACGCTTTT GAAGTTCGTG CTGAAACAGA CTTGAAGATC 
GTCTGCAGAG CCATCCAGCG CTTCCTGTTG ACCTACAAAG AGGAGCGCCG TGGGCCAACT 
CTCATTGCTG TTCAGTCCAA CTGGGACCTG AAGAGGCTGA CTGGCGAGAT CCCTGTCCTG 
GAGGAATTCC CACTGGTGCC TGTCCGCGTG GCTGACAAGA TCAGCTACGG GGTCCTGGAC 
TGGCAGCGCC ACGGAGCCCG GCGCATGATC CGGCACTACC TCAACCTGGA CACGTATCTG 
TCACAGGCCT TCGAGATGAG CAGGTACTTC CACGTCCCCG TTGGGAATCT GCCTGAGGAC 
ATCTCTACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCACC TCCAGCGCCA CAACCACCTG 
CTCTGGCTCT CTCCTACGTC TCGCCCAGAC CTGGGTGGGA AGGAGGCCGA CGACAACAGG 
CTCGTCATGG AGTTTGATGA CCAAGCCCCT GTGGAGATCA ACAGTTCCGG CTGTTACTCC 
ACAGTGTGCG TGGAACTGGA TATTCAGAAC CTGGCCGTCA ACACCATCCT GCAGTCTCAC 
CACGTCAATG ATATGGAGGG GGCCGACAGC ATTGGCATCA GCTTCGATGT GATCCAGCAG 
GCCTCCCTGG AGGACATGAT CACAGGCAAT CAGGCTGCCA GCGCCCCTGC CAGCTATGAC 
GAGACAGCCC TCTGCTCCAA CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG 
AAGGAGATCA CGCAGTACCG CAACGTCTAC GCCGACAACC AGGTGATGCA CTTCTACCGC 
TGGCTCCGGT CGCCGTCCTC GCTGCTGCAC GACCCTGCCC TGCACCGCAC GCTGCACAGC 
ATGATGAAGA AGCTCTTCCT TCAACTCATC GCTGAGTTCA AGCGCCTGGG GTCATCGGTC 
GTCTATGCCA ACTTCAACCG CATCATCCTC TGCACCAAGA AGCGGCGGGT GGAGGACGCC 
CTGGCCTACG TGGAGTACAT CACCAACAGC ATCCACTCTA AAGAGATCTT CCATTCTTTG 
ACAATTTCCT TTTCTCGATG CTGGGAATTT CTTCTCTGGA TGGATCCCTC TAATTATGGT 
GGAATCAAAG GAACTGTTCC ATCTAGGATT CACTGTGGAC AGCAGGACTC CCCAGAAGAG 
GGCAGAGCAC AGCAGGGGGA ACCTGAGGAG GAGGAGGACG AGGACGAGGA GGAGGAGGAG 
GAGGAGGACG AGGGAGGCAC ACAGGAACTG GATGTCGAGG ACTTGCTGGA AAACAACTGG 
AACATCCTGC AGTTCCTGCC CCAGGCAGCC TCCTGCCAGA GCTACTTCCT CATGATCGTT 
TCAGCGTACA TCGTGGCCGT GTACCACAGC ATGAAGGAGG AACTGAGGCG CAGCACCCCA 
GGGAGCACCC CCGTGAGGAG GAGGGGAGCC AGCCAGCTCT CCCAGGAGGC CCAGGGGGAG 
GCCGGAGCCC TTCCTGGAAT GATCACCTTC TCACAAGATT ATGTGGCAAA TGAACTCACT 
CAGAACTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCT 
ACTGAGCTCT CAGAAATGTT TCCTGTTCTC CCCGGTTCTC ACCTGCTGCT CAATAACCCT 
GCTCTGGAGT TCATCAAATA CGTGTGCAAG GTCCTGTCTC TGGACACCAA CATCACAAAC 
CAGGTGAACA AGCTAACCCG AGACCTGCTG CGCCTGGTAG ACGTAGGCGA GTTCTCAGAG 
GAGGCCCAGT TCCGAGACCC CTGCCGTTCC TACGTGCTCC CTGAGGTCAT CTGCCACAAC 
TGTAACTTCT GCCGGGACCT GGACCTGTGC AAAGACCCCT CCTTCTCTCA GGATGGGACC 
GTGCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGTTGCCT ACGACTCATC AGTCATTGAG 
ATGGCCCTGG TGGAAGCCCT ACAGAAGAAG CTGATGGCCT TTACCCTGCA GGACCTGGTC 
TGCCTGAAGT GCCGGGGTGT GAAGGAGACC AACATGGCCG TGTACTGCAG CTGTGCGGGG 
GGCTTCTCCC TCACCATCCA CACTAAGGTC TTCATGGAAC AGATCGGAAT CTTCCAGAAC 
ATCGCCCAGC ACTATGGCAT GTCATACCTC AAGGAGACCC TGGATTGGCT GCAGCTTCAG 
AACCCCCAAG TGGGCCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_005217684.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217741.1 Bos taurus polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

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CloneID	OBa27766
Clone ID Related Accession (Same CDS sequence)	XM_005217684.3
Accession Version	XM_005217684.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	DNA polymerase epsilon catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104452.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG 
ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT 
GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT 
TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC 
TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG 
GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG 
AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA 
ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC 
ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG 
GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT 
GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA 
CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT 
GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC 
GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG 
GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC 
ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA 
CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC 
CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC 
AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC 
CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG 
TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC 
CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG 
TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG 
GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG 
GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC 
CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT 
GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT 
GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG 
CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA 
GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC 
AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC 
GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG 
CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT 
TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC 
GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG 
TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG 
CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC 
ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG 
GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC 
TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT 
GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG 
CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA 
GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG 
GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG 
GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC 
TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG 
GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG 
GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC 
ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT 
GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT 
TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA 
CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG 
GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA 
CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC 
ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA 
AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC 
CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG 
AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC 
TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC 
TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG 
CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG 
CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA 
CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT 
GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC 
AACCAGATGC CCAGCCTCAG TGCCTTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA 
GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC 
TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT 
GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC 
CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG 
GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC 
ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG 
CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC 
GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC 
TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG 
CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC 
AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG 
GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG 
CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG 
GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC 
CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT 
AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GGACTCCCCA 
GAAGAGGGCA GAGCACAGCA GGGGGAACCT GAGGAGGAGG AGGACGAGGA CGAGGAGGAG 
GAGGAGGAGG AGGACGAGGG AGGCACACAG GAACTGGATG TCGAGGACTT GCTGGAAAAC 
AACTGGAACA TCCTGCAGTT CCTGCCCCAG GCAGCCTCCT GCCAGAGCTA CTTCCTCATG 
ATCGTTTCAG CGTACATCGT GGCCGTGTAC CACAGCATGA AGGAGGAACT GAGGCGCAGC 
ACCCCAGGGA GCACCCCCGT GAGGAGGAGG GGAGCCAGCC AGCTCTCCCA GGAGGCCCAG 
GGGGAGGCCG GAGCCCTTCC TGGAATGATC ACCTTCTCAC AAGATTATGT GGCAAATGAA 
CTCACTCAGA ACTTCTTCAC CATCACTCAG AAGATTCAGA AGAAAGTCAC AGGCTCTCGG 
AACTCTACTG AGCTCTCAGA AATGTTTCCT GTTCTCCCCG GTTCTCACCT GCTGCTCAAT 
AACCCTGCTC TGGAGTTCAT CAAATACGTG TGCAAGGTCC TGTCTCTGGA CACCAACATC 
ACAAACCAGG TGAACAAGCT AACCCGAGAC CTGCTGCGCC TGGTAGACGT AGGCGAGTTC 
TCAGAGGAGG CCCAGTTCCG AGACCCCTGC CGTTCCTACG TGCTCCCTGA GGTCATCTGC 
CACAACTGTA ACTTCTGCCG GGACCTGGAC CTGTGCAAAG ACCCCTCCTT CTCTCAGGAT 
GGGACCGTGC TGCCTCAGTG GCTCTGCTCC AACTGTCAGG TTGCCTACGA CTCATCAGTC 
ATTGAGATGG CCCTGGTGGA AGCCCTACAG AAGAAGCTGA TGGCCTTTAC CCTGCAGGAC 
CTGGTCTGCC TGAAGTGCCG GGGTGTGAAG GAGACCAACA TGGCCGTGTA CTGCAGCTGT 
GCGGGGGGCT TCTCCCTCAC CATCCACACT AAGGTCTTCA TGGAACAGAT CGGAATCTTC 
CAGAACATCG CCCAGCACTA TGGCATGTCA TACCTCAAGG AGACCCTGGA TTGGCTGCAG 
CTTCAGAACC CCCAAGTGGG CCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217741.1
CDS	54..6920
Translation	MVLRNTGRLRAEPGADGEPSRDDGSSSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIKLSFNTVEDLVKVRKEISPAVKKNRDQSHTSDAYTTMLSSVLQGGSVIFDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGSAFPVEIARRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEHIQEAKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKSPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEQVINFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCRNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFVLEGKRQVGMAQASEGSQSLGSPDMEDFGLTKPPHSAVPIATKRKRIHCERQEGSQDLELTMPWQEVLGQPPALGTTQEEWLVWLQFHKKKWQLQAQQRLAHKKRRRLEGAEGTPRPGAIREGPSTGLGGFFRRTARSILDLPWQIVQISETSQAGLFRVWAVIGSDLHCIKLNIPRVFYVNQRVAKAEEGPSYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELAAPDIEGVYETQVPLVFRALVQLGCMCVVSKQLVRHLSGWEAETFTLEHLEMRSLAQFNYLEPGSIRHIYLYHHAQGHKALFGIFVPSQRKAAVFVLDTVRSNQMPSLSALYSTEHSLLLEKVGPELLPPQKHAFEVRAETDLKIVCRAIQRFLLTYKEERRGPTLIAVQSNWDLKRLTGEIPVLEEFPLVPVRVADKISYGVLDWQRHGARRMIRHYLNLDTYLSQAFEMSRYFHVPVGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDQAPVEINSSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGADSIGISFDVIQQASLEDMITGNQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYRNVYADNQVMHFYRWLRSPSSLLHDPALHRTLHSMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRVEDALAYVEYITNSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGTVPSRIHCGQDSPEEGRAQQGEPEEEEDEDEEEEEEEDEGGTQELDVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSTPGSTPVRRRGASQLSQEAQGEAGALPGMITFSQDYVANELTQNFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLTRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICHNCNFCRDLDLCKDPSFSQDGTVLPQWLCSNCQVAYDSSVIEMALVEALQKKLMAFTLQDLVCLKCRGVKETNMAVYCSCAGGFSLTIHTKVFMEQIGIFQNIAQHYGMSYLKETLDWLQLQNPQVGH

Target ORF information:

RefSeq Version	XM_005217684.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217684.3

ORF Insert Sequence:

ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG 
ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT 
GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC 
AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT 
TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC 
TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG 
GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG 
AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA 
ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC 
ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG 
GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT 
GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA 
CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT 
GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC 
GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG 
GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC 
ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA 
CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC 
CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC 
AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC 
CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG 
TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC 
CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG 
TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG 
GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG 
GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC 
CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT 
GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT 
GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG 
CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA 
GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC 
AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC 
GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG 
CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT 
TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC 
GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG 
TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG 
CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC 
ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG 
GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC 
TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT 
GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG 
CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA 
GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG 
GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG 
GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC 
TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG 
GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG 
GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC 
ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT 
GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT 
TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA 
CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG 
GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA 
CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC 
ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA 
AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC 
CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG 
AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC 
TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC 
TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG 
CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG 
CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA 
CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT 
GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC 
AACCAGATGC CCAGCCTCAG TGCCTTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA 
GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC 
TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT 
GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC 
CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG 
GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC 
ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG 
CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC 
GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC 
TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG 
CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC 
AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG 
GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG 
CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG 
GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC 
CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT 
AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GGACTCCCCA 
GAAGAGGGCA GAGCACAGCA GGGGGAACCT GAGGAGGAGG AGGACGAGGA CGAGGAGGAG 
GAGGAGGAGG AGGACGAGGG AGGCACACAG GAACTGGATG TCGAGGACTT GCTGGAAAAC 
AACTGGAACA TCCTGCAGTT CCTGCCCCAG GCAGCCTCCT GCCAGAGCTA CTTCCTCATG 
ATCGTTTCAG CGTACATCGT GGCCGTGTAC CACAGCATGA AGGAGGAACT GAGGCGCAGC 
ACCCCAGGGA GCACCCCCGT GAGGAGGAGG GGAGCCAGCC AGCTCTCCCA GGAGGCCCAG 
GGGGAGGCCG GAGCCCTTCC TGGAATGATC ACCTTCTCAC AAGATTATGT GGCAAATGAA 
CTCACTCAGA ACTTCTTCAC CATCACTCAG AAGATTCAGA AGAAAGTCAC AGGCTCTCGG 
AACTCTACTG AGCTCTCAGA AATGTTTCCT GTTCTCCCCG GTTCTCACCT GCTGCTCAAT 
AACCCTGCTC TGGAGTTCAT CAAATACGTG TGCAAGGTCC TGTCTCTGGA CACCAACATC 
ACAAACCAGG TGAACAAGCT AACCCGAGAC CTGCTGCGCC TGGTAGACGT AGGCGAGTTC 
TCAGAGGAGG CCCAGTTCCG AGACCCCTGC CGTTCCTACG TGCTCCCTGA GGTCATCTGC 
CACAACTGTA ACTTCTGCCG GGACCTGGAC CTGTGCAAAG ACCCCTCCTT CTCTCAGGAT 
GGGACCGTGC TGCCTCAGTG GCTCTGCTCC AACTGTCAGG TTGCCTACGA CTCATCAGTC 
ATTGAGATGG CCCTGGTGGA AGCCCTACAG AAGAAGCTGA TGGCCTTTAC CCTGCAGGAC 
CTGGTCTGCC TGAAGTGCCG GGGTGTGAAG GAGACCAACA TGGCCGTGTA CTGCAGCTGT 
GCGGGGGGCT TCTCCCTCAC CATCCACACT AAGGTCTTCA TGGAACAGAT CGGAATCTTC 
CAGAACATCG CCCAGCACTA TGGCATGTCA TACCTCAAGG AGACCCTGGA TTGGCTGCAG 
CTTCAGAACC CCCAAGTGGG CCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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POLE cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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