Pole cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pole
Entrez Gene ID	18973
Full Name	polymerase (DNA directed), epsilon
General protein information	Preferred Names polymerase (DNA directed), epsilon Names DNA polymerase epsilon catalytic subunit A DNA polymerase II subunit A DNA-directed DNA polymerase epsilon pol-epsilon
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 F\|5 53.45 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011132.2	NP_035262.2	DNA polymerase epsilon catalytic subunit A
XM_017320715.1	XP_017176204.1	DNA polymerase epsilon catalytic subunit A isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

Polymerase-mediated ultramutagenesis in mice produces diverse cancers with high mutational load.
Li HD, Cuevas I, Zhang M, Lu C, Alam MM, Fu YX, You MJ, Akbay EA, Zhang H, Castrillon DH
The Journal of clinical investigation128(9)4179-4191(2018 08)

Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis.
Bellelli R, Borel V, Logan C, Svendsen J, Cox DE, Nye E, Metcalfe K, O'Connell SM, Stamp G, Flynn HR, Snijders AP, Lassailly F, Jackson A, Boulton SJ
Molecular cell70(4)707-721.e7(2018 05)

A missense mutation in Rev7 disrupts formation of Polζ, impairing mouse development and repair of genotoxic agent-induced DNA lesions.
Khalaj M, Abbasi A, Yamanishi H, Akiyama K, Wakitani S, Kikuchi S, Hirose M, Yuzuriha M, Magari M, Degheidy HA, Abe K, Ogura A, Hashimoto H, Kunieda T
The Journal of biological chemistry289(6)3811-24(2014 Feb)

DNA polymerase epsilon and delta proofreading suppress discrete mutator and cancer phenotypes in mice.
Albertson TM, Ogawa M, Bugni JM, Hays LE, Chen Y, Wang Y, Treuting PM, Heddle JA, Goldsby RE, Preston BD
Proceedings of the National Academy of Sciences of the United States of America106(40)17101-4(2009 Oct)

Mammalian TIMELESS and Tipin are evolutionarily conserved replication fork-associated factors.
Gotter AL, Suppa C, Emanuel BS
Journal of molecular biology366(1)36-52(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pole gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pole cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17798	NM_011132.2 Latest version!	Mus musculus polymerase (DNA directed), epsilon (Pole), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu81271	XM_017320715.1 Latest version!	Mus musculus polymerase (DNA directed), epsilon (Pole), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pole ( NM_011132.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035262.2 Mus musculus polymerase (DNA directed), epsilon (Pole), mRNA.

Price & Availability↑

CloneID	OMu17798
Clone ID Related Accession (Same CDS sequence)	NM_011132.2
Accession Version	NM_011132.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Mus musculus(house mouse)
Product	DNA polymerase epsilon catalytic subunit A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ101052.1, AK141674.1 and BC048166.1. On Aug 7, 2008 this sequence version replaced NM_011132.1. ##Evidence-Data-START## Transcript exon combination :: BC048166.1, AF123502.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGGTCCTGA GGAACAGTGG ACGGAGGCAC CCCGAGCCGG GCGCGGATGG CGAAGGCAGC 
CGGGATGATG GTCCCTCTTC CTCAGTCTCA GCACTCAAGC GTCTGGAACG GAGCCAGTGG 
ACAGACAAGA TGGACTTACG GTTTGGTTTC GAAAGGCTGA AAGAGCCTGG AGAAAGGACT 
GGCTGGCTGA TCAACATGCA CCCTACTGAG ATCTTAGATG AAGACAAACG CTTAGTCAGC 
GCGGTGGATT ACTACTTCAT TCAAGATGAT GGAAGCAGAT TTAAGGTGGC CTTGCCCTAT 
ATGCCGTATT TCTACATTGC AGCGAGAAAG GGTTGTGATC GAGAAGTTTC ATCTTTTCTA 
TCCAAGAAGT TTCAGGGAAA AATTGCAAAG TTAGAGAATG TGCCCAAAGA AGATCTGGAC 
TTGCCAAATC ACTTGGTGGG CTTGAAGCGG AGTTACATCA AGCTGTCCTT CCACACTGTG 
GAGGACCTTG TCAAAGTGAG AAAGGAGATC TCTCCTGCTG TGAAGAAGAA CCGAGAGCAG 
GACCATGCTA GTGATGAGTA TACAACAATG CTCTCCAGTA TTCTGCAAGG TGGCAGTGTA 
ATTACTGATG AGGACGAAAC CTCTAAGAAG ATAGCTGACC AATTGGACAA CATAGTGGAC 
ATGCGAGAGT ATGATGTTCC CTACCACATT CGCCTCTCCA TTGACCTCAG AATCCATGTG 
GCCCACTGGT ACAATGTTAG ATTTCGAGGA AATGCTTTTC CTGTGGAAAT CACCCGACGA 
GATGATCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATCGA GACGACCAAA 
CTGCCTCTCA AATTCCCTGA TGCTGAGACC GATCAGATCA TGATGATCTC CTATATGATT 
GATGGCCAGG GCTACCTCAT CACTAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CTAAGCCAGA ATATGAAGGG CCCTTTTGTG TCTTCAATGA ACCCGACGAG 
GTCCATCTGA TCCAGAGATG GTTTGAGCAT ATCCAGGAAA CCAAACCTAC CATTATGGTC 
ACCTACAATG GGGATTTTTT TGACTGGCCA TTTGTGGAGG CTAGGGCAGC AATTCATGGC 
CTCAGCATGT ACCAGGAGAT AGGCTTCCAG AAGGATAGCC AGGGGGAATA TAAGGCACCA 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCAGC TGCCAAGGCC AAACTTGGCT ATGACCCTGT AGAGCTGGAC 
CCTGAGGACA TGTGTCGTAT GGCCACTGAA CAGCCCCAGA CTCTGGCCAC TTACTCAGTG 
TCAGATGCTG TGGCTACTTA CTACCTGTAC ATGAAATACG TCCACCCCTT CATATTCGCC 
CTGTGCACCA TTATTCCCAT GGAACCTGAT GAGGTGCTGC GGAAGGGCTC CGGGACACTG 
TGTGAAGCCT TGCTGATGGT GCAAGCTTTC CATGCCAACA TTATCTTCCC CAATAAGCAA 
GAGCAGGAGT TCAACAAGCT GACAGATGAT GGCCACATGC TAGATGCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCACT AGAGTCTGGT GTCTTCAGAA GTGATATCCC CTGCCGGTTT 
AGGATGAATC CTGCAGCCTT TGATTTCCTG CTGCAACGAG TCGAGAAGAC TATGCGCCAC 
GCCATTGAAG AAGAAGAGAA GGTGCCTGTG GAACAAGCCA CCAACTTTCA AGAGGTGTGT 
GAGCAGATTA AGACCAAGCT CACCTCCCTA AAAGATGTTC CTAACAGAAT TGAATGTCCT 
CTAATCTACC ATCTAGATGT GGGGGCCATG TATCCTAACA TAATTCTTAC CAACCGCCTA 
CAGCCTTCTG CCATAGTGGA TGAGGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAGTTGTC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AATTCATGCC AGCCAGTCGC 
AGTGAATACC ATCGGATTCA GCATCAGCTG GAGTCGGAGA AGTTTCCCCC TTTGTTTCCA 
GAGGGGCCAG CACGGGCCTT TCACGAGCTG TCCCGTGAAG AACAGGCTAA ATACGAGAAG 
AGGAGGCTGG CAGATTATTG CCGGAAAGCC TATAAGAAGA TCCATGTGAC CAAGGTAGAA 
GAACGTCTAA CTACCATCTG CCAGCGGGAA AACTCATTTT ATGTGGACAC AGTGCGGGCC 
TTCAGAGACA GGCGCTATGA GTTCAAAGGA CTGCACAAGG TGTGGAAGAA GAAGCTCTCG 
GCAGCTGTAG AGGTGGGCGA TGCATCAGAG GTGAAGCGCT GCAAGAACAT GGAGATCCTT 
TACGATTCAC TGCAGCTGGC TCACAAGTGC ATCCTGAACT CCTTCTACGG CTATGTCATG 
CGCAAAGGAG CTCGCTGGTA TTCCATGGAG ATGGCTGGTA TCGTCTGCTT TACAGGAGCC 
AACATCATCA CCCAAGCAAG AGAACTGATT GAGCAGATCG GGAGGCCTTT AGAATTGGAC 
ACGGACGGAA TATGGTGCGT CCTACCCAAT AGCTTTCCTG AAAATTTTGT CATCAAGACA 
ACCAATGCGA AGAAACCCAA ACTGACCATC TCCTATCCTG GTGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTTACCAA CCACCAGTAC CAGGAACTAA CAGAGCCTTC GTCTCTCACC 
TATGTCACCC ACTCTGAGAA TAGTATCTTT TTTGAAGTCG ATGGACCATA CCTTGCTATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAGCTGAAGA AAAGATATGC TGTGTTCAAT 
GAAGATGGTT CCTTGGCTGA ACTGAAAGGT TTTGAGGTGA AACGCCGAGG GGAGTTGCAG 
CTGATTAAAA TATTCCAGTC CTCAGTTTTT GAGGCCTTCC TCAAGGGCAG CACACTGGAG 
GAAGTGTATG GCTCGGTGGC CAAAGTGGCT GACTACTGGC TAGATGTGCT CTATAGCAAG 
GCTGCTAATA TGCCCGATTC TGAATTGTTT GAGCTGATTT CTGAGAACCG CTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACATCCA TCAGCACAGC AAAGCGCCTG 
GCTGAGTTCC TGGGAGACCA GATGGTCAAA GATGCTGGAC TGAGCTGCCG CTATATCATC 
TCCCGAAAGC CAGAGGGGTC TCCTGTCACT GAGAGGGCCA TTCCACTTGC CATTTTCCAA 
GCAGAGCCTA CAGTGAGGAA ACATTTTCTC CGGAAATGGC TAAAGAGTTC ATCACTTCAA 
GACTTTGATA TTCGGACAAT TCTGGACTGG GACTACTACA TAGAGAGGCT GGGGAGTGCC 
ATCCAGAAAA TCATCACCAT CCCCGCAGCT CTGCAGCAGG TGAAGAACCC AGTTCCACGT 
GTCAAACATC CAGACTGGCT ACACAAAAAA CTACTAGAGA AGAATGATAT CTACAAACAG 
AAGAAGATCA GTGAGCTCTT TGTGCTTGAA GGAAAGAGAC AGATTGTGAT GGCCCAGGCT 
TCAGAAAACA GTCTGAGTCT CTGCACTCCA GACATGGAGG ACATTGGACT CACAAAGCCA 
CACCACTCTA CAGTCCCAGT TGCTACTAAG AGGAAGCGAG TCTGGGAGAC CCAAAAGGAG 
TCTCAGGATA TTGCACTAAC TGTGCCCTGG CAAGAGGTCT TAGGGCAGCC TCCCTCCCTT 
GGAACCACAC AGGAAGAGTG GTTGGTCTGG CTCCAGTTCC ACAAGAAAAA GTGGCAGCTG 
CAGGCCCAAC AGCGCCTAGC CCTCAGGAAG AAGCAACGCT TAGAGTCAGC AGAAGATATG 
CCAAGGCTTG GGCCTATCCG AGAGGGGCCT TCCACAGGAC TGGGGAGCTT TTTGCGAAGG 
ACTGCCCGCA GCATCATGGA CCTTCCATGG CAGATAATAC AGATCAGTGA GACCAGACAG 
GCTGGTCTGT TCCGGCTGTG GGCTATCATT GGCAATGACT TGCACTGCAT CAAGCTGAGT 
ATCCCTCGAG TATTCTATGT AAACCAGCGG GTTGCCAAAG CAGAGGATGG ACCTGCATAT 
CGGAAGGTGA ATCGGGCTCT TCCTCGTTCC AACATTGTCT ACAATCTCTA TGAGTATTCA 
GTACCAGAGG ACATGTACCA AGAACACATC AACGAGATCA ACACTGAGTT GTCAGTACCA 
GACATTGAGG GCGTGTATGA GACACAGGTC CCATTGTTAT TCCGGGCCCT CGTGCAGCTG 
GGCTGTGTGT GTGTGGTCAA CAAGCAGCTG ACAAGGCACC TTTCGGGCTG GGAAGCTGAA 
ACTTTTGCCC TCGAGCACCT TGAAATGCGT TCTCTGGCCC AGTTCAGCTA CTTGGAACCA 
GGGAGTATCC GCCATATCTA CCTGTACCAT CACACTCAGG GCCACAAGGC ACTCTTTGGG 
GTCTTTATCC CCTCACAGCG AAGAGCATCT GTGTTTGTGT TGGATACTGT ACGAAGCAAC 
CAAATGCCAG GGCTCAGTGC CCTGTACTCA TCAGAACACA GCCTGCTGCT GGACAAGGTG 
GACCCCAAGC TCCTGCCTCC CCCAAAACAC ACCTTTGAAG TTCGTGCTGA AACCAACCTG 
AAGACTATCT GCAGAGCCAT CCAGCGCTTC CTGCTTGCCT ACAAGGAAGA GCGCCGAGGG 
CCCACACTCA TCGCTGTCCA GTCTAGCTGG GAGCTGTGTA GGCTGACCAG TGAGATTCCA 
GTCTTAGAAG AGTTCCCACT AGTGCCTATC CGAGTGGCTG ACAAGATCAG CTATGCAGTC 
CTGGACTGGC AGCGCCATGG AGCTCGCCGA ATGATCCGGC ACTACCTCAA TTTAGACTTG 
TGCCTGTCGC AGGCCTTTGA GATGAGCAGG TACTTCCACA TCCCTGTTGG AAACCTGCCG 
GAAGACATCT CCACCTTTGG CTCAGACCTC TTTTTTGCAC GCCACCTCCA GCACCATAAC 
CACCTGCTTT GGCTATCCCC TACCTCTCGG CCTGACCTGG GTGGGAAGGA AGCTGATGAC 
AACCGCCTTG TCATGGAGTT TGATGACCGA GCCACTGTGG AGATCAATAG TTCTGGCTGT 
TACTCTACTG TGTGCGTGGA ACTGGACATT CAAAATCTGG CAGTCAACAC CATCCTCCAG 
TCCCATCATG TCAATGACAT GGAGGGGGCT GGCAGCATGG GCATCAGCTT CGATGTGATC 
CAGCAGGCCT CCCTAGAGGA CATGGTAACA GGCAATCAAG CTGCCAGTGC CCTGGCCAAC 
TACGATGAGA CAGCCCTCTG CTCTAGTACC TTCAGGATCC TGAAGAGCAT GGTGGTTGGC 
TGGGTAAAGG AAATCACACA GTACCACAAC ATCTATGCTG ACAACCAGGT AATGCACTTC 
TACCGCTGGC TCCAGTCACC GTGCTCTCTG CTCCACGACC CAGCCCTTCA CCGGACGCTG 
CACAACATGA TGAAGAAGCT CTTCCTGCAG CTCATTGCTG AGTTCAAGCG CCTGGGGTCA 
TCAGTCGTCT ATGCCAACTT CAATCGCATC ATTCTCTGTA CAAAGAAGCG CCGAATAGAG 
GATGCCCTTG CCTATGTGGA ATATATTACC AACAGCATCC ATTCTAAAGA GATCTTCCAT 
TCCCTGACCA TCTCTTTCTC TCGATGCTGG GAATTCCTTC TCTGGATGGA TCCATCCAAC 
TATGGTGGAA TCAAAGGAAA AGTTCCATCT AGTATTCACT GTGGACAGGT AAAAGAGCAA 
GACTCCCAGG CAAGAGAGGA AACTGATGAA GAGGAGGAGG ACAAGGAAAA GGACGAGGAG 
GAAGAGGGCA TGGGAGAGTC CGAGGTTGAG GACTTACTGG AGAACAACTG GAACATTCTA 
CAGTTCTTGC CCCAGGCAGC CTCTTGCCAG AGCTACTTCC TCATGATTGT TTCAGCATAC 
ATCGTAGCTG TGTACCAAAG CATGAAGGAG GAGTTGAGAC ACAGTGCCCC GGGCAGTACC 
CCTGTGAAGA GGAAGGGGGC CAGCCAGTTC TCCCAGGAGT CTGAAGGGGC AACTGGATCT 
CTTCCTGGAA TGATCACTTT CTCTCAAGAT TATGTGGCAA ATGAGCTCAC TCAGAGCTTC 
TTCACCATTA CTCAGAAAAT TCAGAAGAAA GTCACAGGTT CTCGGAACAC CACTGAGCCC 
TCAGAGATGT TCCCCGTCCT CCCTGGTTCA CACTTGCTGC TCAATAATCC TGCTCTGGAG 
TTCATCAAAT ATGTGTGCAA GGTACTATCT CTGGATACAA ACATCACAAA TCAGGTGAAT 
AAGCTGAACA GAGACCTTCT TCGCCTGGTA GACGTTGGTG AATTCTCTGA GGAGGCCCAG 
TTCAGAGACC CCTGCCACTC CTACGTGCTC CCTGAGGTAA TCTGCCACAG CTGTAATTTC 
TGCCGAGACC TGGACCTGTG CAAAGATTCC TCTTTCTCTC AGGATGGAGC CATCCTGCCT 
CAGTGGCTCT GCTCCAATTG TCAAGCCCCC TATGACTCCT CTGCCATTGA GTCAGCCTTG 
GTGGAAGCCC TGCAGAGGAA ACTGATGGCC TTCACACTTC AGGACCTGGT ATGCCTCAAG 
TGCCGTGGTA TGAAAGAGAC CCATATGCCT GTGTACTGCA GCTGCGCAGG GGACTTTACT 
CTCACCATCC GCACTGAGGT CTTCATGGAA CAGATTAGAA TCTTCCAGAA CATTGCCAAG 
TACTACAGCA TGTCATATCT CCAGGAGACC ATAGAATGGC TGTTACAGAC AAGCCCTGTA 
TCAAACTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035262.2
CDS	70..6921
Translation	MVLRNSGRRHPEPGADGEGSRDDGPSSSVSALKRLERSQWTDKMDLRFGFERLKEPGERTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYMPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLDLPNHLVGLKRSYIKLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSVITDEDETSKKIADQLDNIVDMREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEHIQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHMLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAIVDEATCAACDFNKPGASCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNAKKPKLTISYPGAMLNIMVKEGFTNHQYQELTEPSSLTYVTHSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDIYKQKKISELFVLEGKRQIVMAQASENSLSLCTPDMEDIGLTKPHHSTVPVATKRKRVWETQKESQDIALTVPWQEVLGQPPSLGTTQEEWLVWLQFHKKKWQLQAQQRLALRKKQRLESAEDMPRLGPIREGPSTGLGSFLRRTARSIMDLPWQIIQISETRQAGLFRLWAIIGNDLHCIKLSIPRVFYVNQRVAKAEDGPAYRKVNRALPRSNIVYNLYEYSVPEDMYQEHINEINTELSVPDIEGVYETQVPLLFRALVQLGCVCVVNKQLTRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHTQGHKALFGVFIPSQRRASVFVLDTVRSNQMPGLSALYSSEHSLLLDKVDPKLLPPPKHTFEVRAETNLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELCRLTSEIPVLEEFPLVPIRVADKISYAVLDWQRHGARRMIRHYLNLDLCLSQAFEMSRYFHIPVGNLPEDISTFGSDLFFARHLQHHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDRATVEINSSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGAGSMGISFDVIQQASLEDMVTGNQAASALANYDETALCSSTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLQSPCSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDALAYVEYITNSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGKVPSSIHCGQVKEQDSQAREETDEEEEDKEKDEEEEGMGESEVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYQSMKEELRHSAPGSTPVKRKGASQFSQESEGATGSLPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNTTEPSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCHSYVLPEVICHSCNFCRDLDLCKDSSFSQDGAILPQWLCSNCQAPYDSSAIESALVEALQRKLMAFTLQDLVCLKCRGMKETHMPVYCSCAGDFTLTIRTEVFMEQIRIFQNIAKYYSMSYLQETIEWLLQTSPVSNC

Target ORF information:

RefSeq Version	NM_011132.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), epsilon (Pole), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011132.2

ORF Insert Sequence:

ATGGTCCTGA GGAACAGTGG ACGGAGGCAC CCCGAGCCGG GCGCGGATGG CGAAGGCAGC 
CGGGATGATG GTCCCTCTTC CTCAGTCTCA GCACTCAAGC GTCTGGAACG GAGCCAGTGG 
ACAGACAAGA TGGACTTACG GTTTGGTTTC GAAAGGCTGA AAGAGCCTGG AGAAAGGACT 
GGCTGGCTGA TCAACATGCA CCCTACTGAG ATCTTAGATG AAGACAAACG CTTAGTCAGC 
GCGGTGGATT ACTACTTCAT TCAAGATGAT GGAAGCAGAT TTAAGGTGGC CTTGCCCTAT 
ATGCCGTATT TCTACATTGC AGCGAGAAAG GGTTGTGATC GAGAAGTTTC ATCTTTTCTA 
TCCAAGAAGT TTCAGGGAAA AATTGCAAAG TTAGAGAATG TGCCCAAAGA AGATCTGGAC 
TTGCCAAATC ACTTGGTGGG CTTGAAGCGG AGTTACATCA AGCTGTCCTT CCACACTGTG 
GAGGACCTTG TCAAAGTGAG AAAGGAGATC TCTCCTGCTG TGAAGAAGAA CCGAGAGCAG 
GACCATGCTA GTGATGAGTA TACAACAATG CTCTCCAGTA TTCTGCAAGG TGGCAGTGTA 
ATTACTGATG AGGACGAAAC CTCTAAGAAG ATAGCTGACC AATTGGACAA CATAGTGGAC 
ATGCGAGAGT ATGATGTTCC CTACCACATT CGCCTCTCCA TTGACCTCAG AATCCATGTG 
GCCCACTGGT ACAATGTTAG ATTTCGAGGA AATGCTTTTC CTGTGGAAAT CACCCGACGA 
GATGATCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATCGA GACGACCAAA 
CTGCCTCTCA AATTCCCTGA TGCTGAGACC GATCAGATCA TGATGATCTC CTATATGATT 
GATGGCCAGG GCTACCTCAT CACTAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CTAAGCCAGA ATATGAAGGG CCCTTTTGTG TCTTCAATGA ACCCGACGAG 
GTCCATCTGA TCCAGAGATG GTTTGAGCAT ATCCAGGAAA CCAAACCTAC CATTATGGTC 
ACCTACAATG GGGATTTTTT TGACTGGCCA TTTGTGGAGG CTAGGGCAGC AATTCATGGC 
CTCAGCATGT ACCAGGAGAT AGGCTTCCAG AAGGATAGCC AGGGGGAATA TAAGGCACCA 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCAGC TGCCAAGGCC AAACTTGGCT ATGACCCTGT AGAGCTGGAC 
CCTGAGGACA TGTGTCGTAT GGCCACTGAA CAGCCCCAGA CTCTGGCCAC TTACTCAGTG 
TCAGATGCTG TGGCTACTTA CTACCTGTAC ATGAAATACG TCCACCCCTT CATATTCGCC 
CTGTGCACCA TTATTCCCAT GGAACCTGAT GAGGTGCTGC GGAAGGGCTC CGGGACACTG 
TGTGAAGCCT TGCTGATGGT GCAAGCTTTC CATGCCAACA TTATCTTCCC CAATAAGCAA 
GAGCAGGAGT TCAACAAGCT GACAGATGAT GGCCACATGC TAGATGCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCACT AGAGTCTGGT GTCTTCAGAA GTGATATCCC CTGCCGGTTT 
AGGATGAATC CTGCAGCCTT TGATTTCCTG CTGCAACGAG TCGAGAAGAC TATGCGCCAC 
GCCATTGAAG AAGAAGAGAA GGTGCCTGTG GAACAAGCCA CCAACTTTCA AGAGGTGTGT 
GAGCAGATTA AGACCAAGCT CACCTCCCTA AAAGATGTTC CTAACAGAAT TGAATGTCCT 
CTAATCTACC ATCTAGATGT GGGGGCCATG TATCCTAACA TAATTCTTAC CAACCGCCTA 
CAGCCTTCTG CCATAGTGGA TGAGGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAGTTGTC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AATTCATGCC AGCCAGTCGC 
AGTGAATACC ATCGGATTCA GCATCAGCTG GAGTCGGAGA AGTTTCCCCC TTTGTTTCCA 
GAGGGGCCAG CACGGGCCTT TCACGAGCTG TCCCGTGAAG AACAGGCTAA ATACGAGAAG 
AGGAGGCTGG CAGATTATTG CCGGAAAGCC TATAAGAAGA TCCATGTGAC CAAGGTAGAA 
GAACGTCTAA CTACCATCTG CCAGCGGGAA AACTCATTTT ATGTGGACAC AGTGCGGGCC 
TTCAGAGACA GGCGCTATGA GTTCAAAGGA CTGCACAAGG TGTGGAAGAA GAAGCTCTCG 
GCAGCTGTAG AGGTGGGCGA TGCATCAGAG GTGAAGCGCT GCAAGAACAT GGAGATCCTT 
TACGATTCAC TGCAGCTGGC TCACAAGTGC ATCCTGAACT CCTTCTACGG CTATGTCATG 
CGCAAAGGAG CTCGCTGGTA TTCCATGGAG ATGGCTGGTA TCGTCTGCTT TACAGGAGCC 
AACATCATCA CCCAAGCAAG AGAACTGATT GAGCAGATCG GGAGGCCTTT AGAATTGGAC 
ACGGACGGAA TATGGTGCGT CCTACCCAAT AGCTTTCCTG AAAATTTTGT CATCAAGACA 
ACCAATGCGA AGAAACCCAA ACTGACCATC TCCTATCCTG GTGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTTACCAA CCACCAGTAC CAGGAACTAA CAGAGCCTTC GTCTCTCACC 
TATGTCACCC ACTCTGAGAA TAGTATCTTT TTTGAAGTCG ATGGACCATA CCTTGCTATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAGCTGAAGA AAAGATATGC TGTGTTCAAT 
GAAGATGGTT CCTTGGCTGA ACTGAAAGGT TTTGAGGTGA AACGCCGAGG GGAGTTGCAG 
CTGATTAAAA TATTCCAGTC CTCAGTTTTT GAGGCCTTCC TCAAGGGCAG CACACTGGAG 
GAAGTGTATG GCTCGGTGGC CAAAGTGGCT GACTACTGGC TAGATGTGCT CTATAGCAAG 
GCTGCTAATA TGCCCGATTC TGAATTGTTT GAGCTGATTT CTGAGAACCG CTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACATCCA TCAGCACAGC AAAGCGCCTG 
GCTGAGTTCC TGGGAGACCA GATGGTCAAA GATGCTGGAC TGAGCTGCCG CTATATCATC 
TCCCGAAAGC CAGAGGGGTC TCCTGTCACT GAGAGGGCCA TTCCACTTGC CATTTTCCAA 
GCAGAGCCTA CAGTGAGGAA ACATTTTCTC CGGAAATGGC TAAAGAGTTC ATCACTTCAA 
GACTTTGATA TTCGGACAAT TCTGGACTGG GACTACTACA TAGAGAGGCT GGGGAGTGCC 
ATCCAGAAAA TCATCACCAT CCCCGCAGCT CTGCAGCAGG TGAAGAACCC AGTTCCACGT 
GTCAAACATC CAGACTGGCT ACACAAAAAA CTACTAGAGA AGAATGATAT CTACAAACAG 
AAGAAGATCA GTGAGCTCTT TGTGCTTGAA GGAAAGAGAC AGATTGTGAT GGCCCAGGCT 
TCAGAAAACA GTCTGAGTCT CTGCACTCCA GACATGGAGG ACATTGGACT CACAAAGCCA 
CACCACTCTA CAGTCCCAGT TGCTACTAAG AGGAAGCGAG TCTGGGAGAC CCAAAAGGAG 
TCTCAGGATA TTGCACTAAC TGTGCCCTGG CAAGAGGTCT TAGGGCAGCC TCCCTCCCTT 
GGAACCACAC AGGAAGAGTG GTTGGTCTGG CTCCAGTTCC ACAAGAAAAA GTGGCAGCTG 
CAGGCCCAAC AGCGCCTAGC CCTCAGGAAG AAGCAACGCT TAGAGTCAGC AGAAGATATG 
CCAAGGCTTG GGCCTATCCG AGAGGGGCCT TCCACAGGAC TGGGGAGCTT TTTGCGAAGG 
ACTGCCCGCA GCATCATGGA CCTTCCATGG CAGATAATAC AGATCAGTGA GACCAGACAG 
GCTGGTCTGT TCCGGCTGTG GGCTATCATT GGCAATGACT TGCACTGCAT CAAGCTGAGT 
ATCCCTCGAG TATTCTATGT AAACCAGCGG GTTGCCAAAG CAGAGGATGG ACCTGCATAT 
CGGAAGGTGA ATCGGGCTCT TCCTCGTTCC AACATTGTCT ACAATCTCTA TGAGTATTCA 
GTACCAGAGG ACATGTACCA AGAACACATC AACGAGATCA ACACTGAGTT GTCAGTACCA 
GACATTGAGG GCGTGTATGA GACACAGGTC CCATTGTTAT TCCGGGCCCT CGTGCAGCTG 
GGCTGTGTGT GTGTGGTCAA CAAGCAGCTG ACAAGGCACC TTTCGGGCTG GGAAGCTGAA 
ACTTTTGCCC TCGAGCACCT TGAAATGCGT TCTCTGGCCC AGTTCAGCTA CTTGGAACCA 
GGGAGTATCC GCCATATCTA CCTGTACCAT CACACTCAGG GCCACAAGGC ACTCTTTGGG 
GTCTTTATCC CCTCACAGCG AAGAGCATCT GTGTTTGTGT TGGATACTGT ACGAAGCAAC 
CAAATGCCAG GGCTCAGTGC CCTGTACTCA TCAGAACACA GCCTGCTGCT GGACAAGGTG 
GACCCCAAGC TCCTGCCTCC CCCAAAACAC ACCTTTGAAG TTCGTGCTGA AACCAACCTG 
AAGACTATCT GCAGAGCCAT CCAGCGCTTC CTGCTTGCCT ACAAGGAAGA GCGCCGAGGG 
CCCACACTCA TCGCTGTCCA GTCTAGCTGG GAGCTGTGTA GGCTGACCAG TGAGATTCCA 
GTCTTAGAAG AGTTCCCACT AGTGCCTATC CGAGTGGCTG ACAAGATCAG CTATGCAGTC 
CTGGACTGGC AGCGCCATGG AGCTCGCCGA ATGATCCGGC ACTACCTCAA TTTAGACTTG 
TGCCTGTCGC AGGCCTTTGA GATGAGCAGG TACTTCCACA TCCCTGTTGG AAACCTGCCG 
GAAGACATCT CCACCTTTGG CTCAGACCTC TTTTTTGCAC GCCACCTCCA GCACCATAAC 
CACCTGCTTT GGCTATCCCC TACCTCTCGG CCTGACCTGG GTGGGAAGGA AGCTGATGAC 
AACCGCCTTG TCATGGAGTT TGATGACCGA GCCACTGTGG AGATCAATAG TTCTGGCTGT 
TACTCTACTG TGTGCGTGGA ACTGGACATT CAAAATCTGG CAGTCAACAC CATCCTCCAG 
TCCCATCATG TCAATGACAT GGAGGGGGCT GGCAGCATGG GCATCAGCTT CGATGTGATC 
CAGCAGGCCT CCCTAGAGGA CATGGTAACA GGCAATCAAG CTGCCAGTGC CCTGGCCAAC 
TACGATGAGA CAGCCCTCTG CTCTAGTACC TTCAGGATCC TGAAGAGCAT GGTGGTTGGC 
TGGGTAAAGG AAATCACACA GTACCACAAC ATCTATGCTG ACAACCAGGT AATGCACTTC 
TACCGCTGGC TCCAGTCACC GTGCTCTCTG CTCCACGACC CAGCCCTTCA CCGGACGCTG 
CACAACATGA TGAAGAAGCT CTTCCTGCAG CTCATTGCTG AGTTCAAGCG CCTGGGGTCA 
TCAGTCGTCT ATGCCAACTT CAATCGCATC ATTCTCTGTA CAAAGAAGCG CCGAATAGAG 
GATGCCCTTG CCTATGTGGA ATATATTACC AACAGCATCC ATTCTAAAGA GATCTTCCAT 
TCCCTGACCA TCTCTTTCTC TCGATGCTGG GAATTCCTTC TCTGGATGGA TCCATCCAAC 
TATGGTGGAA TCAAAGGAAA AGTTCCATCT AGTATTCACT GTGGACAGGT AAAAGAGCAA 
GACTCCCAGG CAAGAGAGGA AACTGATGAA GAGGAGGAGG ACAAGGAAAA GGACGAGGAG 
GAAGAGGGCA TGGGAGAGTC CGAGGTTGAG GACTTACTGG AGAACAACTG GAACATTCTA 
CAGTTCTTGC CCCAGGCAGC CTCTTGCCAG AGCTACTTCC TCATGATTGT TTCAGCATAC 
ATCGTAGCTG TGTACCAAAG CATGAAGGAG GAGTTGAGAC ACAGTGCCCC GGGCAGTACC 
CCTGTGAAGA GGAAGGGGGC CAGCCAGTTC TCCCAGGAGT CTGAAGGGGC AACTGGATCT 
CTTCCTGGAA TGATCACTTT CTCTCAAGAT TATGTGGCAA ATGAGCTCAC TCAGAGCTTC 
TTCACCATTA CTCAGAAAAT TCAGAAGAAA GTCACAGGTT CTCGGAACAC CACTGAGCCC 
TCAGAGATGT TCCCCGTCCT CCCTGGTTCA CACTTGCTGC TCAATAATCC TGCTCTGGAG 
TTCATCAAAT ATGTGTGCAA GGTACTATCT CTGGATACAA ACATCACAAA TCAGGTGAAT 
AAGCTGAACA GAGACCTTCT TCGCCTGGTA GACGTTGGTG AATTCTCTGA GGAGGCCCAG 
TTCAGAGACC CCTGCCACTC CTACGTGCTC CCTGAGGTAA TCTGCCACAG CTGTAATTTC 
TGCCGAGACC TGGACCTGTG CAAAGATTCC TCTTTCTCTC AGGATGGAGC CATCCTGCCT 
CAGTGGCTCT GCTCCAATTG TCAAGCCCCC TATGACTCCT CTGCCATTGA GTCAGCCTTG 
GTGGAAGCCC TGCAGAGGAA ACTGATGGCC TTCACACTTC AGGACCTGGT ATGCCTCAAG 
TGCCGTGGTA TGAAAGAGAC CCATATGCCT GTGTACTGCA GCTGCGCAGG GGACTTTACT 
CTCACCATCC GCACTGAGGT CTTCATGGAA CAGATTAGAA TCTTCCAGAA CATTGCCAAG 
TACTACAGCA TGTCATATCT CCAGGAGACC ATAGAATGGC TGTTACAGAC AAGCCCTGTA 
TCAAACTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pole ( XM_017320715.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176204.1 Mus musculus polymerase (DNA directed), epsilon (Pole), transcript variant X1, mRNA.

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CloneID	OMu81271
Clone ID Related Accession (Same CDS sequence)	XM_017320715.1
Accession Version	XM_017320715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	DNA polymerase epsilon catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTATT TCTACATTGC AGCGAGAAAG GGTTGTGATC GAGAAGTTTC ATCTTTTCTA 
TCCAAGAAGT TTCAGGGAAA AATTGCAAAG TTAGAGAATG TGCCCAAAGA AGATCTGGAC 
TTGCCAAATC ACTTGGTGGG CTTGAAGCGG AGTTACATCA AGCTGTCCTT CCACACTGTG 
GAGGACCTTG TCAAAGTGAG AAAGGAGATC TCTCCTGCTG TGAAGAAGAA CCGAGAGCAG 
GACCATGCTA GTGATGAGTA TACAACAATG CTCTCCAGTA TTCTGCAAGG TGGCAGTGTA 
ATTACTGATG AGGACGAAAC CTCTAAGAAG ATAGCTGACC AATTGGACAA CATAGTGGAC 
ATGCGAGAGT ATGATGTTCC CTACCACATT CGCCTCTCCA TTGACCTCAG AATCCATGTG 
GCCCACTGGT ACAATGTTAG ATTTCGAGGA AATGCTTTTC CTGTGGAAAT CACCCGACGA 
GATGATCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATCGA GACGACCAAA 
CTGCCTCTCA AATTCCCTGA TGCTGAGACC GATCAGATCA TGATGATCTC CTATATGATT 
GATGGCCAGG GCTACCTCAT CACTAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CTAAGCCAGA ATATGAAGGG CCCTTTTGTG TCTTCAATGA ACCCGACGAG 
GTCCATCTGA TCCAGAGATG GTTTGAGCAT ATCCAGGAAA CCAAACCTAC CATTATGGTC 
ACCTACAATG GGGATTTTTT TGACTGGCCA TTTGTGGAGG CTAGGGCAGC AATTCATGGC 
CTCAGCATGT ACCAGGAGAT AGGCTTCCAG AAGGATAGCC AGGGGGAATA TAAGGCACCA 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCAGC TGCCAAGGCC AAACTTGGCT ATGACCCTGT AGAGCTGGAC 
CCTGAGGACA TGTGTCGTAT GGCCACTGAA CAGCCCCAGA CTCTGGCCAC TTACTCAGTG 
TCAGATGCTG TGGCTACTTA CTACCTGTAC ATGAAATACG TCCACCCCTT CATATTCGCC 
CTGTGCACCA TTATTCCCAT GGAACCTGAT GAGGTGCTGC GGAAGGGCTC CGGGACACTG 
TGTGAAGCCT TGCTGATGGT GCAAGCTTTC CATGCCAACA TTATCTTCCC CAATAAGCAA 
GAGCAGGAGT TCAACAAGCT GACAGATGAT GGCCACATGC TAGATGCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCACT AGAGTCTGGT GTCTTCAGAA GTGATATCCC CTGCCGGTTT 
AGGATGAATC CTGCAGCCTT TGATTTCCTG CTGCAACGAG TCGAGAAGAC TATGCGCCAC 
GCCATTGAAG AAGAAGAGAA GGTGCCTGTG GAACAAGCCA CCAACTTTCA AGAGGTGTGT 
GAGCAGATTA AGACCAAGCT CACCTCCCTA AAAGATGTTC CTAACAGAAT TGAATGTCCT 
CTAATCTACC ATCTAGATGT GGGGGCCATG TATCCTAACA TAATTCTTAC CAACCGCCTA 
CAGCCTTCTG CCATAGTGGA TGAGGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAGTTGTC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AATTCATGCC AGCCAGTCGC 
AGTGAATACC ATCGGATTCA GCATCAGCTG GAGTCGGAGA AGTTTCCCCC TTTGTTTCCA 
GAGGGGCCAG CACGGGCCTT TCACGAGCTG TCCCGTGAAG AACAGGCTAA ATACGAGAAG 
AGGAGGCTGG CAGATTATTG CCGGAAAGCC TATAAGAAGA TCCATGTGAC CAAGGTAGAA 
GAACGTCTAA CTACCATCTG CCAGCGGGAA AACTCATTTT ATGTGGACAC AGTGCGGGCC 
TTCAGAGACA GGCGCTATGA GTTCAAAGGA CTGCACAAGG TGTGGAAGAA GAAGCTCTCG 
GCAGCTGTAG AGGTGGGCGA TGCATCAGAG GTGAAGCGCT GCAAGAACAT GGAGATCCTT 
TACGATTCAC TGCAGCTGGC TCACAAGTGC ATCCTGAACT CCTTCTACGG CTATGTCATG 
CGCAAAGGAG CTCGCTGGTA TTCCATGGAG ATGGCTGGTA TCGTCTGCTT TACAGGAGCC 
AACATCATCA CCCAAGCAAG AGAACTGATT GAGCAGATCG GGAGGCCTTT AGAATTGGAC 
ACGGACGGAA TATGGTGCGT CCTACCCAAT AGCTTTCCTG AAAATTTTGT CATCAAGACA 
ACCAATGCGA AGAAACCCAA ACTGACCATC TCCTATCCTG GTGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTTACCAA CCACCAGTAC CAGGAACTAA CAGAGCCTTC GTCTCTCACC 
TATGTCACCC ACTCTGAGAA TAGTATCTTT TTTGAAGTCG ATGGACCATA CCTTGCTATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAGCTGAAGA AAAGATATGC TGTGTTCAAT 
GAAGATGGTT CCTTGGCTGA ACTGAAAGGT TTTGAGGTGA AACGCCGAGG GGAGTTGCAG 
CTGATTAAAA TATTCCAGTC CTCAGTTTTT GAGGCCTTCC TCAAGGGCAG CACACTGGAG 
GAAGTGTATG GCTCGGTGGC CAAAGTGGCT GACTACTGGC TAGATGTGCT CTATAGCAAG 
GCTGCTAATA TGCCCGATTC TGAATTGTTT GAGCTGATTT CTGAGAACCG CTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACATCCA TCAGCACAGC AAAGCGCCTG 
GCTGAGTTCC TGGGAGACCA GATGGTCAAA GATGCTGGAC TGAGCTGCCG CTATATCATC 
TCCCGAAAGC CAGAGGGGTC TCCTGTCACT GAGAGGGCCA TTCCACTTGC CATTTTCCAA 
GCAGAGCCTA CAGTGAGGAA ACATTTTCTC CGGAAATGGC TAAAGAGTTC ATCACTTCAA 
GACTTTGATA TTCGGACAAT TCTGGACTGG GACTACTACA TAGAGAGGCT GGGGAGTGCC 
ATCCAGAAAA TCATCACCAT CCCCGCAGCT CTGCAGCAGG TGAAGAACCC AGTTCCACGT 
GTCAAACATC CAGACTGGCT ACACAAAAAA CTACTAGAGA AGAATGATAT CTACAAACAG 
AAGAAGATCA GTGAGCTCTT TGTGCTTGAA GGAAAGAGAC AGATTGTGAT GGCCCAGGCT 
TCAGAAAACA GTCTGAGTCT CTGCACTCCA GACATGGAGG ACATTGGACT CACAAAGCCA 
CACCACTCTA CAGTCCCAGT TGCTACTAAG AGGAAGCGAG TCTGGGAGAC CCAAAAGGAG 
TCTCAGGATA TTGCACTAAC TGTGCCCTGG CAAGAGGTCT TAGGGCAGCC TCCCTCCCTT 
GGAACCACAC AGGAAGAGTG GTTGGTCTGG CTCCAGTTCC ACAAGAAAAA GTGGCAGCTG 
CAGGCCCAAC AGCGCCTAGC CCTCAGGAAG AAGCAACGCT TAGAGTCAGC AGAAGATATG 
CCAAGGCTTG GGCCTATCCG AGAGGGGCCT TCCACAGGAC TGGGGAGCTT TTTGCGAAGG 
ACTGCCCGCA GCATCATGGA CCTTCCATGG CAGATAATAC AGATCAGTGA GACCAGACAG 
GCTGGTCTGT TCCGGCTGTG GGCTATCATT GGCAATGACT TGCACTGCAT CAAGCTGAGT 
ATCCCTCGAG TATTCTATGT AAACCAGCGG GTTGCCAAAG CAGAGGATGG ACCTGCATAT 
CGGAAGGTGA ATCGGGCTCT TCCTCGTTCC AACATTGTCT ACAATCTCTA TGAGTATTCA 
GTACCAGAGG ACATGTACCA AGAACACATC AACGAGATCA ACACTGAGTT GTCAGTACCA 
GACATTGAGG GCGTGTATGA GACACAGGTC CCATTGTTAT TCCGGGCCCT CGTGCAGCTG 
GGCTGTGTGT GTGTGGTCAA CAAGCAGCTG ACAAGGCACC TTTCGGGCTG GGAAGCTGAA 
ACTTTTGCCC TCGAGCACCT TGAAATGCGT TCTCTGGCCC AGTTCAGCTA CTTGGAACCA 
GGGAGTATCC GCCATATCTA CCTGTACCAT CACACTCAGG GCCACAAGGC ACTCTTTGGG 
GTCTTTATCC CCTCACAGCG AAGAGCATCT GTGTTTGTGT TGGATACTGT ACGAAGCAAC 
CAAATGCCAG GGCTCAGTGC CCTGTACTCA TCAGAACACA GCCTGCTGCT GGACAAGGTG 
GACCCCAAGC TCCTGCCTCC CCCAAAACAC ACCTTTGAAG TTCGTGCTGA AACCAACCTG 
AAGACTATCT GCAGAGCCAT CCAGCGCTTC CTGCTTGCCT ACAAGGAAGA GCGCCGAGGG 
CCCACACTCA TCGCTGTCCA GTCTAGCTGG GAGCTGTGTA GGCTGACCAG TGAGATTCCA 
GTCTTAGAAG AGTTCCCACT AGTGCCTATC CGAGTGGCTG ACAAGATCAG CTATGCAGTC 
CTGGACTGGC AGCGCCATGG AGCTCGCCGA ATGATCCGGC ACTACCTCAA TTTAGACTTG 
TGCCTGTCGC AGGCCTTTGA GATGAGCAGG TACTTCCACA TCCCTGTTGG AAACCTGCCG 
GAAGACATCT CCACCTTTGG CTCAGACCTC TTTTTTGCAC GCCACCTCCA GCACCATAAC 
CACCTGCTTT GGCTATCCCC TACCTCTCGG CCTGACCTGG GTGGGAAGGA AGCTGATGAC 
AACCGCCTTG TCATGGAGTT TGATGACCGA GCCACTGTGG AGATCAATAG TTCTGGCTGT 
TACTCTACTG TGTGCGTGGA ACTGGACATT CAAAATCTGG CAGTCAACAC CATCCTCCAG 
TCCCATCATG TCAATGACAT GGAGGGGGCT GGCAGCATGG GCATCAGCTT CGATGTGATC 
CAGCAGGCCT CCCTAGAGGA CATGGTAACA GGCAATCAAG CTGCCAGTGC CCTGGCCAAC 
TACGATGAGA CAGCCCTCTG CTCTAGTACC TTCAGGATCC TGAAGAGCAT GGTGGTTGGC 
TGGGTAAAGG AAATCACACA GTACCACAAC ATCTATGCTG ACAACCAGGT AATGCACTTC 
TACCGCTGGC TCCAGTCACC GTGCTCTCTG CTCCACGACC CAGCCCTTCA CCGGACGCTG 
CACAACATGA TGAAGAAGCT CTTCCTGCAG CTCATTGCTG AGTTCAAGCG CCTGGGGTCA 
TCAGTCGTCT ATGCCAACTT CAATCGCATC ATTCTCTGTA CAAAGAAGCG CCGAATAGAG 
GATGCCCTTG CCTATGTGGA ATATATTACC AACAGCATCC ATTCTAAAGA GATCTTCCAT 
TCCCTGACCA TCTCTTTCTC TCGATGCTGG GAATTCCTTC TCTGGATGGA TCCATCCAAC 
TATGGTGGAA TCAAAGGAAA AGTTCCATCT AGTATTCACT GTGGACAGGT AAAAGAGCAA 
GACTCCCAGG CAAGAGAGGA AACTGATGAA GAGGAGGAGG ACAAGGAAAA GGACGAGGAG 
GAAGAGGGCA TGGGAGAGTC CGAGGTTGAG GACTTACTGG AGAACAACTG GAACATTCTA 
CAGTTCTTGC CCCAGGCAGC CTCTTGCCAG AGCTACTTCC TCATGATTGT TTCAGCATAC 
ATCGTAGCTG TGTACCAAAG CATGAAGGAG GAGTTGAGAC ACAGTGCCCC GGGCAGTACC 
CCTGTGAAGA GGAAGGGGGC CAGCCAGTTC TCCCAGGAGT CTGAAGGGGC AACTGGATCT 
CTTCCTGGAA TGATCACTTT CTCTCAAGAT TATGTGGCAA ATGAGCTCAC TCAGAGCTTC 
TTCACCATTA CTCAGAAAAT TCAGAAGAAA GTCACAGGTT CTCGGAACAC CACTGAGCCC 
TCAGAGATGT TCCCCGTCCT CCCTGGTTCA CACTTGCTGC TCAATAATCC TGCTCTGGAG 
TTCATCAAAT ATGTGTGCAA GGTACTATCT CTGGATACAA ACATCACAAA TCAGGTGAAT 
AAGCTGAACA GAGACCTTCT TCGCCTGGTA GACGTTGGTG AATTCTCTGA GGAGGCCCAG 
TTCAGAGACC CCTGCCACTC CTACGTGCTC CCTGAGGTAA TCTGCCACAG CTGTAATTTC 
TGCCGAGACC TGGACCTGTG CAAAGATTCC TCTTTCTCTC AGGATGGAGC CATCCTGCCT 
CAGTGGCTCT GCTCCAATTG TCAAGCCCCC TATGACTCCT CTGCCATTGA GTCAGCCTTG 
GTGGAAGCCC TGCAGAGGAA ACTGATGGCC TTCACACTTC AGGACCTGGT ATGCCTCAAG 
TGCCGTGGTA TGAAAGAGAC CCATATGCCT GTGTACTGCA GCTGCGCAGG GGACTTTACT 
CTCACCATCC GCACTGAGGT CTTCATGGAA CAGATTAGAA TCTTCCAGAA CATTGCCAAG 
TACTACAGCA TGTCATATCT CCAGGAGACC ATAGAATGGC TGTTACAGAC AAGCCCTGTA 
TCAAACTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176204.1
CDS	344..6895
Translation	MPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLDLPNHLVGLKRSYIKLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSVITDEDETSKKIADQLDNIVDMREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEHIQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHMLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAIVDEATCAACDFNKPGASCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNAKKPKLTISYPGAMLNIMVKEGFTNHQYQELTEPSSLTYVTHSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDIYKQKKISELFVLEGKRQIVMAQASENSLSLCTPDMEDIGLTKPHHSTVPVATKRKRVWETQKESQDIALTVPWQEVLGQPPSLGTTQEEWLVWLQFHKKKWQLQAQQRLALRKKQRLESAEDMPRLGPIREGPSTGLGSFLRRTARSIMDLPWQIIQISETRQAGLFRLWAIIGNDLHCIKLSIPRVFYVNQRVAKAEDGPAYRKVNRALPRSNIVYNLYEYSVPEDMYQEHINEINTELSVPDIEGVYETQVPLLFRALVQLGCVCVVNKQLTRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHTQGHKALFGVFIPSQRRASVFVLDTVRSNQMPGLSALYSSEHSLLLDKVDPKLLPPPKHTFEVRAETNLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELCRLTSEIPVLEEFPLVPIRVADKISYAVLDWQRHGARRMIRHYLNLDLCLSQAFEMSRYFHIPVGNLPEDISTFGSDLFFARHLQHHNHLLWLSPTSRPDLGGKEADDNRLVMEFDDRATVEINSSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGAGSMGISFDVIQQASLEDMVTGNQAASALANYDETALCSSTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLQSPCSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDALAYVEYITNSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGKVPSSIHCGQVKEQDSQAREETDEEEEDKEKDEEEEGMGESEVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYQSMKEELRHSAPGSTPVKRKGASQFSQESEGATGSLPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNTTEPSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCHSYVLPEVICHSCNFCRDLDLCKDSSFSQDGAILPQWLCSNCQAPYDSSAIESALVEALQRKLMAFTLQDLVCLKCRGMKETHMPVYCSCAGDFTLTIRTEVFMEQIRIFQNIAKYYSMSYLQETIEWLLQTSPVSNC

Target ORF information:

RefSeq Version	XM_017320715.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA directed), epsilon (Pole), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320715.1

ORF Insert Sequence:

ATGCCGTATT TCTACATTGC AGCGAGAAAG GGTTGTGATC GAGAAGTTTC ATCTTTTCTA 
TCCAAGAAGT TTCAGGGAAA AATTGCAAAG TTAGAGAATG TGCCCAAAGA AGATCTGGAC 
TTGCCAAATC ACTTGGTGGG CTTGAAGCGG AGTTACATCA AGCTGTCCTT CCACACTGTG 
GAGGACCTTG TCAAAGTGAG AAAGGAGATC TCTCCTGCTG TGAAGAAGAA CCGAGAGCAG 
GACCATGCTA GTGATGAGTA TACAACAATG CTCTCCAGTA TTCTGCAAGG TGGCAGTGTA 
ATTACTGATG AGGACGAAAC CTCTAAGAAG ATAGCTGACC AATTGGACAA CATAGTGGAC 
ATGCGAGAGT ATGATGTTCC CTACCACATT CGCCTCTCCA TTGACCTCAG AATCCATGTG 
GCCCACTGGT ACAATGTTAG ATTTCGAGGA AATGCTTTTC CTGTGGAAAT CACCCGACGA 
GATGATCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATCGA GACGACCAAA 
CTGCCTCTCA AATTCCCTGA TGCTGAGACC GATCAGATCA TGATGATCTC CTATATGATT 
GATGGCCAGG GCTACCTCAT CACTAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CTAAGCCAGA ATATGAAGGG CCCTTTTGTG TCTTCAATGA ACCCGACGAG 
GTCCATCTGA TCCAGAGATG GTTTGAGCAT ATCCAGGAAA CCAAACCTAC CATTATGGTC 
ACCTACAATG GGGATTTTTT TGACTGGCCA TTTGTGGAGG CTAGGGCAGC AATTCATGGC 
CTCAGCATGT ACCAGGAGAT AGGCTTCCAG AAGGATAGCC AGGGGGAATA TAAGGCACCA 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCAGC TGCCAAGGCC AAACTTGGCT ATGACCCTGT AGAGCTGGAC 
CCTGAGGACA TGTGTCGTAT GGCCACTGAA CAGCCCCAGA CTCTGGCCAC TTACTCAGTG 
TCAGATGCTG TGGCTACTTA CTACCTGTAC ATGAAATACG TCCACCCCTT CATATTCGCC 
CTGTGCACCA TTATTCCCAT GGAACCTGAT GAGGTGCTGC GGAAGGGCTC CGGGACACTG 
TGTGAAGCCT TGCTGATGGT GCAAGCTTTC CATGCCAACA TTATCTTCCC CAATAAGCAA 
GAGCAGGAGT TCAACAAGCT GACAGATGAT GGCCACATGC TAGATGCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCACT AGAGTCTGGT GTCTTCAGAA GTGATATCCC CTGCCGGTTT 
AGGATGAATC CTGCAGCCTT TGATTTCCTG CTGCAACGAG TCGAGAAGAC TATGCGCCAC 
GCCATTGAAG AAGAAGAGAA GGTGCCTGTG GAACAAGCCA CCAACTTTCA AGAGGTGTGT 
GAGCAGATTA AGACCAAGCT CACCTCCCTA AAAGATGTTC CTAACAGAAT TGAATGTCCT 
CTAATCTACC ATCTAGATGT GGGGGCCATG TATCCTAACA TAATTCTTAC CAACCGCCTA 
CAGCCTTCTG CCATAGTGGA TGAGGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAGTTGTC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AATTCATGCC AGCCAGTCGC 
AGTGAATACC ATCGGATTCA GCATCAGCTG GAGTCGGAGA AGTTTCCCCC TTTGTTTCCA 
GAGGGGCCAG CACGGGCCTT TCACGAGCTG TCCCGTGAAG AACAGGCTAA ATACGAGAAG 
AGGAGGCTGG CAGATTATTG CCGGAAAGCC TATAAGAAGA TCCATGTGAC CAAGGTAGAA 
GAACGTCTAA CTACCATCTG CCAGCGGGAA AACTCATTTT ATGTGGACAC AGTGCGGGCC 
TTCAGAGACA GGCGCTATGA GTTCAAAGGA CTGCACAAGG TGTGGAAGAA GAAGCTCTCG 
GCAGCTGTAG AGGTGGGCGA TGCATCAGAG GTGAAGCGCT GCAAGAACAT GGAGATCCTT 
TACGATTCAC TGCAGCTGGC TCACAAGTGC ATCCTGAACT CCTTCTACGG CTATGTCATG 
CGCAAAGGAG CTCGCTGGTA TTCCATGGAG ATGGCTGGTA TCGTCTGCTT TACAGGAGCC 
AACATCATCA CCCAAGCAAG AGAACTGATT GAGCAGATCG GGAGGCCTTT AGAATTGGAC 
ACGGACGGAA TATGGTGCGT CCTACCCAAT AGCTTTCCTG AAAATTTTGT CATCAAGACA 
ACCAATGCGA AGAAACCCAA ACTGACCATC TCCTATCCTG GTGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTTACCAA CCACCAGTAC CAGGAACTAA CAGAGCCTTC GTCTCTCACC 
TATGTCACCC ACTCTGAGAA TAGTATCTTT TTTGAAGTCG ATGGACCATA CCTTGCTATG 
ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAGCTGAAGA AAAGATATGC TGTGTTCAAT 
GAAGATGGTT CCTTGGCTGA ACTGAAAGGT TTTGAGGTGA AACGCCGAGG GGAGTTGCAG 
CTGATTAAAA TATTCCAGTC CTCAGTTTTT GAGGCCTTCC TCAAGGGCAG CACACTGGAG 
GAAGTGTATG GCTCGGTGGC CAAAGTGGCT GACTACTGGC TAGATGTGCT CTATAGCAAG 
GCTGCTAATA TGCCCGATTC TGAATTGTTT GAGCTGATTT CTGAGAACCG CTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACATCCA TCAGCACAGC AAAGCGCCTG 
GCTGAGTTCC TGGGAGACCA GATGGTCAAA GATGCTGGAC TGAGCTGCCG CTATATCATC 
TCCCGAAAGC CAGAGGGGTC TCCTGTCACT GAGAGGGCCA TTCCACTTGC CATTTTCCAA 
GCAGAGCCTA CAGTGAGGAA ACATTTTCTC CGGAAATGGC TAAAGAGTTC ATCACTTCAA 
GACTTTGATA TTCGGACAAT TCTGGACTGG GACTACTACA TAGAGAGGCT GGGGAGTGCC 
ATCCAGAAAA TCATCACCAT CCCCGCAGCT CTGCAGCAGG TGAAGAACCC AGTTCCACGT 
GTCAAACATC CAGACTGGCT ACACAAAAAA CTACTAGAGA AGAATGATAT CTACAAACAG 
AAGAAGATCA GTGAGCTCTT TGTGCTTGAA GGAAAGAGAC AGATTGTGAT GGCCCAGGCT 
TCAGAAAACA GTCTGAGTCT CTGCACTCCA GACATGGAGG ACATTGGACT CACAAAGCCA 
CACCACTCTA CAGTCCCAGT TGCTACTAAG AGGAAGCGAG TCTGGGAGAC CCAAAAGGAG 
TCTCAGGATA TTGCACTAAC TGTGCCCTGG CAAGAGGTCT TAGGGCAGCC TCCCTCCCTT 
GGAACCACAC AGGAAGAGTG GTTGGTCTGG CTCCAGTTCC ACAAGAAAAA GTGGCAGCTG 
CAGGCCCAAC AGCGCCTAGC CCTCAGGAAG AAGCAACGCT TAGAGTCAGC AGAAGATATG 
CCAAGGCTTG GGCCTATCCG AGAGGGGCCT TCCACAGGAC TGGGGAGCTT TTTGCGAAGG 
ACTGCCCGCA GCATCATGGA CCTTCCATGG CAGATAATAC AGATCAGTGA GACCAGACAG 
GCTGGTCTGT TCCGGCTGTG GGCTATCATT GGCAATGACT TGCACTGCAT CAAGCTGAGT 
ATCCCTCGAG TATTCTATGT AAACCAGCGG GTTGCCAAAG CAGAGGATGG ACCTGCATAT 
CGGAAGGTGA ATCGGGCTCT TCCTCGTTCC AACATTGTCT ACAATCTCTA TGAGTATTCA 
GTACCAGAGG ACATGTACCA AGAACACATC AACGAGATCA ACACTGAGTT GTCAGTACCA 
GACATTGAGG GCGTGTATGA GACACAGGTC CCATTGTTAT TCCGGGCCCT CGTGCAGCTG 
GGCTGTGTGT GTGTGGTCAA CAAGCAGCTG ACAAGGCACC TTTCGGGCTG GGAAGCTGAA 
ACTTTTGCCC TCGAGCACCT TGAAATGCGT TCTCTGGCCC AGTTCAGCTA CTTGGAACCA 
GGGAGTATCC GCCATATCTA CCTGTACCAT CACACTCAGG GCCACAAGGC ACTCTTTGGG 
GTCTTTATCC CCTCACAGCG AAGAGCATCT GTGTTTGTGT TGGATACTGT ACGAAGCAAC 
CAAATGCCAG GGCTCAGTGC CCTGTACTCA TCAGAACACA GCCTGCTGCT GGACAAGGTG 
GACCCCAAGC TCCTGCCTCC CCCAAAACAC ACCTTTGAAG TTCGTGCTGA AACCAACCTG 
AAGACTATCT GCAGAGCCAT CCAGCGCTTC CTGCTTGCCT ACAAGGAAGA GCGCCGAGGG 
CCCACACTCA TCGCTGTCCA GTCTAGCTGG GAGCTGTGTA GGCTGACCAG TGAGATTCCA 
GTCTTAGAAG AGTTCCCACT AGTGCCTATC CGAGTGGCTG ACAAGATCAG CTATGCAGTC 
CTGGACTGGC AGCGCCATGG AGCTCGCCGA ATGATCCGGC ACTACCTCAA TTTAGACTTG 
TGCCTGTCGC AGGCCTTTGA GATGAGCAGG TACTTCCACA TCCCTGTTGG AAACCTGCCG 
GAAGACATCT CCACCTTTGG CTCAGACCTC TTTTTTGCAC GCCACCTCCA GCACCATAAC 
CACCTGCTTT GGCTATCCCC TACCTCTCGG CCTGACCTGG GTGGGAAGGA AGCTGATGAC 
AACCGCCTTG TCATGGAGTT TGATGACCGA GCCACTGTGG AGATCAATAG TTCTGGCTGT 
TACTCTACTG TGTGCGTGGA ACTGGACATT CAAAATCTGG CAGTCAACAC CATCCTCCAG 
TCCCATCATG TCAATGACAT GGAGGGGGCT GGCAGCATGG GCATCAGCTT CGATGTGATC 
CAGCAGGCCT CCCTAGAGGA CATGGTAACA GGCAATCAAG CTGCCAGTGC CCTGGCCAAC 
TACGATGAGA CAGCCCTCTG CTCTAGTACC TTCAGGATCC TGAAGAGCAT GGTGGTTGGC 
TGGGTAAAGG AAATCACACA GTACCACAAC ATCTATGCTG ACAACCAGGT AATGCACTTC 
TACCGCTGGC TCCAGTCACC GTGCTCTCTG CTCCACGACC CAGCCCTTCA CCGGACGCTG 
CACAACATGA TGAAGAAGCT CTTCCTGCAG CTCATTGCTG AGTTCAAGCG CCTGGGGTCA 
TCAGTCGTCT ATGCCAACTT CAATCGCATC ATTCTCTGTA CAAAGAAGCG CCGAATAGAG 
GATGCCCTTG CCTATGTGGA ATATATTACC AACAGCATCC ATTCTAAAGA GATCTTCCAT 
TCCCTGACCA TCTCTTTCTC TCGATGCTGG GAATTCCTTC TCTGGATGGA TCCATCCAAC 
TATGGTGGAA TCAAAGGAAA AGTTCCATCT AGTATTCACT GTGGACAGGT AAAAGAGCAA 
GACTCCCAGG CAAGAGAGGA AACTGATGAA GAGGAGGAGG ACAAGGAAAA GGACGAGGAG 
GAAGAGGGCA TGGGAGAGTC CGAGGTTGAG GACTTACTGG AGAACAACTG GAACATTCTA 
CAGTTCTTGC CCCAGGCAGC CTCTTGCCAG AGCTACTTCC TCATGATTGT TTCAGCATAC 
ATCGTAGCTG TGTACCAAAG CATGAAGGAG GAGTTGAGAC ACAGTGCCCC GGGCAGTACC 
CCTGTGAAGA GGAAGGGGGC CAGCCAGTTC TCCCAGGAGT CTGAAGGGGC AACTGGATCT 
CTTCCTGGAA TGATCACTTT CTCTCAAGAT TATGTGGCAA ATGAGCTCAC TCAGAGCTTC 
TTCACCATTA CTCAGAAAAT TCAGAAGAAA GTCACAGGTT CTCGGAACAC CACTGAGCCC 
TCAGAGATGT TCCCCGTCCT CCCTGGTTCA CACTTGCTGC TCAATAATCC TGCTCTGGAG 
TTCATCAAAT ATGTGTGCAA GGTACTATCT CTGGATACAA ACATCACAAA TCAGGTGAAT 
AAGCTGAACA GAGACCTTCT TCGCCTGGTA GACGTTGGTG AATTCTCTGA GGAGGCCCAG 
TTCAGAGACC CCTGCCACTC CTACGTGCTC CCTGAGGTAA TCTGCCACAG CTGTAATTTC 
TGCCGAGACC TGGACCTGTG CAAAGATTCC TCTTTCTCTC AGGATGGAGC CATCCTGCCT 
CAGTGGCTCT GCTCCAATTG TCAAGCCCCC TATGACTCCT CTGCCATTGA GTCAGCCTTG 
GTGGAAGCCC TGCAGAGGAA ACTGATGGCC TTCACACTTC AGGACCTGGT ATGCCTCAAG 
TGCCGTGGTA TGAAAGAGAC CCATATGCCT GTGTACTGCA GCTGCGCAGG GGACTTTACT 
CTCACCATCC GCACTGAGGT CTTCATGGAA CAGATTAGAA TCTTCCAGAA CATTGCCAAG 
TACTACAGCA TGTCATATCT CCAGGAGACC ATAGAATGGC TGTTACAGAC AAGCCCTGTA 
TCAAACTGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polymerase-mediated ultramutagenesis in mice produces diverse cancers with high mutational load.
The Journal of clinical investigation128(9)4179-4191(2018 08)
Li HD,Cuevas I,Zhang M,Lu C,Alam MM,Fu YX,You MJ,Akbay EA,Zhang H,Castrillon DH

Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis.
Molecular cell70(4)707-721.e7(2018 05)
Bellelli R,Borel V,Logan C,Svendsen J,Cox DE,Nye E,Metcalfe K,O'Connell SM,Stamp G,Flynn HR,Snijders AP,Lassailly F,Jackson A,Boulton SJ

A missense mutation in Rev7 disrupts formation of Polζ, impairing mouse development and repair of genotoxic agent-induced DNA lesions.
The Journal of biological chemistry289(6)3811-24(2014 Feb)
Khalaj M,Abbasi A,Yamanishi H,Akiyama K,Wakitani S,Kikuchi S,Hirose M,Yuzuriha M,Magari M,Degheidy HA,Abe K,Ogura A,Hashimoto H,Kunieda T

DNA polymerase epsilon and delta proofreading suppress discrete mutator and cancer phenotypes in mice.
Proceedings of the National Academy of Sciences of the United States of America106(40)17101-4(2009 Oct)
Albertson TM,Ogawa M,Bugni JM,Hays LE,Chen Y,Wang Y,Treuting PM,Heddle JA,Goldsby RE,Preston BD

Mammalian TIMELESS and Tipin are evolutionarily conserved replication fork-associated factors.
Journal of molecular biology366(1)36-52(2007 Feb)
Gotter AL,Suppa C,Emanuel BS

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Pole cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

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