POLE cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLE
Entrez Gene ID	416997
Full Name	DNA polymerase epsilon, catalytic subunit
General protein information	Preferred Names DNA polymerase epsilon, catalytic subunit Names DNA polymerase epsilon catalytic subunit A polymerase (DNA directed), epsilon, catalytic subunit polymerase (DNA) epsilon, catalytic subunit
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001233984.4	XP_001233985.3	DNA polymerase epsilon catalytic subunit A
XM_001233984.5	XP_001233985.3	DNA polymerase epsilon catalytic subunit A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology

The following POLE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa15806	XM_001233984.5 Latest version!	Gallus gallus DNA polymerase epsilon, catalytic subunit (POLE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OGa15806	XM_001233984.4	Gallus gallus polymerase (DNA directed), epsilon, catalytic subunit (POLE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLE ( XM_001233984.4 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_001233985.3 Gallus gallus polymerase (DNA directed), epsilon, catalytic subunit (POLE), mRNA.

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CloneID	OGa15806
Clone ID Related Accession (Same CDS sequence)	XM_001233984.4 , XM_001233984.5
Accession Version	XM_001233984.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	DNA polymerase epsilon catalytic subunit A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471461.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_001233984.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGC GGGGCGGCGG CGGCGGCGAC CGTGACCCCG AGGGCACCCG GGATGATGGC 
CCCGCGCTGT CTGCCCTCAA GCGCCTGGAG CGGTCGCAGC GCACGGACCA GTTGGACGCG 
CTGTTCGGCT TCGAGCGACC CGCCGAGCCC TGCGAGCGGA CGGGCTGGCT CATCAACATG 
CACCCGACCG AGGTGCTGGA TGAGGACCGG CGGGCAGTGA GCGCTGTGGA CTATTACTTC 
ATCCAGGAGG ATGGCAGCCG CTTCAAGGTG GCCCTGCCCT ACAGACCCTA CTTCTATGTG 
GCCACCCAGC AGGGCTGCGA GCGGGAGGTG TCCTCCTTCC TGGCCAAGAA GTTCCAAGGG 
AAGATTGCGA AGCTGGAAAC TGTCCCAAAG GAAGATTTGG ATCTGCCCAA CCACCTGGTG 
GGGCTGAAGC GGCACTACCT CAAGCTGTCC TTCAGCACAG TGGATGAGTT GGTGAAGGTG 
AGGAAAGAGA TCTCCCCTGC TGTAAGGAAG AACAGGGAGC GCGACCAGGC CAATGATGTG 
TACACATCCA TGCTGTCAAG TGCTCTGGCT GGTGGCAGCC TGACCACATC GGAGGAGGGA 
GCCTCCAAAA AGGTCACCAA TCAAATGGAC AACATTGTGG ACATGCGGGA GTACGACGTG 
CCCTACCACA TGCGTCTCTC CATCGACCTC AAGATCCACG TGGCTCACTG GTACAACGTG 
CGGTACCGCG GCAGCACCTT CCCCCCCGAG ATCACCCGCA GGGATGACCT GGTGGAGCGG 
CCGGATCCTG TTGTCCTCGC CTTTGACATT GAAACCACAA AACTCCCCTT GAAGTTTCCT 
GATCCAGAGA CGGACCAGAT CATGATGATC TCCTACATGA TTGATGGGCA GGGCTACCTG 
ATCACAAATA GGGAGATCGT CTCTGAGGAT ATCGAAGACT TTGAGTTCAC TCCCAAGCCG 
GAGTACGAGG GTCCCTTTTG TGTCTTTAAC GAGCCAGATG AAGCTCATTT AATCCAGAGA 
TGGTTTGAAC ACGTCCAGGA GACCAAACCT ACCATCATTG TCACATACAA TGGAGACTTC 
TTTGACTGGC CCTTTGTAGA AGCAAGAGCC GCAGCTCATG GAATTAACAT GTACCAGGAA 
ATTGGATTTC AGAAGGACAG CCAGGGAGAG TACAAAGCAT CACAGTGCAT CCACATGGAC 
TGCCTAAGGT GGGTGAAGAG AGACAGCTAC CTCCCAGTGG GAAGTCACAA CCTGAAGGCA 
GCAGCTAAAG CAAAACTGGG CTATGACCCA GTGGAGCTGG ACCCTGAAGA GATGTGCAGG 
ATGGCTACAG AGGAGCCTCA GACCCTGGCC ACCTATTCAG TGTCCGATGC TGTTGCCACC 
TATTACATGT ATATGAAGTA TGTGCACCCT TTCATTTTTG CCTTGTGCAC CATCATTCCC 
ATGGAGCCCG ATGAGGTGTT ACGAAAAGGT TCTGGGACGC TGTGCGAGGC GCTGCTGATG 
GTTCAAGCCT ATCATGCCAA CATCATCTTC CCCAACAAGC AGGAGCAGGA ATTCAACAAG 
CTCACAGAGG ATGGCCACGT GCTGGACTCG GAGACGTACG TTGGAGGCCA CGTGGAAGCA 
CTGGAGTCGG GGGTGTTCCG CAGTGACATC CCCTGCCGCT TCAAAATGAA CCCTGCTGCC 
TTTGACTACT TGGTGCAGAA CGTGGAGAAG ACGCTCCGGC ACGCTATTGA GGAGGAGGAG 
CGTTTACCGT TGGATCAAGT CACCAACTTC CAGGAGGTCT GTGATGAAAT CAAAGTCAAA 
CTGAATTCCC TGAAGGATGT TCCTAACCGA ATTGAGTGTC CCCTTATCTA CCATTTAGAT 
GTGGGAGCCA TGTACCCCAA CATCATTCTG ACCAACAGAT TGCAGCCCTC AGCAATGGTG 
GATGAGGCCA CATGTGCTGC CTGTGACTTC AACAAGCCAG GGGCCAACTG CCAGCGCCGG 
ATGACGTGGC AATGGAGAGG AGAATTCATG TCTGCGAGCC GCAGTGAGTA CCACCGCATC 
CAGCAGCAAT TGGAGTCGGA GAAGTTCCCT CCACTGTACC CTGATGGAGC ACCCCGTGCT 
TTCCACGAGC TCTCCCGAGA AGAACAAGCC AAGTATGAGA AGAAACGGCT GGCAGATTAC 
TGTCGCAAAG CCTACAAGAA GATCCATGTC ACCAAGGTGG AGGAGAGGGT CACCACCATC 
TGCCAACGAG AGAACTCTTT CTATGTGGAC ACGGTGCGAG CTTTCAGGGA CCGCCGCTAT 
GAGTTCAAGG GGCTGCACAA GGTATGGAAG AAGAAGCTGT CTGCAGCCAT GGAGACAGGA 
GATGCCTCTG AAGTGAAACG CTGCAAGAAC ATGGAGATCC TCTATGACTC CCTGCAGTTG 
GCACACAAGT GCATTCTCAA TTCTTTCTAT GGCTATGTCA TGCGGAAGGG AGCTCGATGG 
TACTCCATGG AAATGGCCGG GATTGTCTGC TTCACCGGAG CCAATATCAT CACACAGGCC 
AGGGAACTGA TTGAGCAGAT TGGGAGACCT CTGGAACTGG ATACAGATGG GATTTGGTGT 
GTCCTCCCCA ACAGCTTCCC AGAAAACTTT GTCATCAAGT CAACCAATGC CAAGAAGCCA 
AAGGTGACAA TCTCCTACCC AGGTGCTATG CTGAACATCC TGGTGAAGGA AGGCTTCACA 
AACGATCAGT ACCAGGAGCT GCAGGACCCA GCCTCTCTCA CCTACGTCAC ACGCTCGGAG 
AACAGCATCT TCTTTGAAGT GGATGGGCCA TACCTGGCCA TGATTCTCCC AGCTTCCAAG 
GAGGAGGGAA AGAAGCTGAA GAAAAGGTAT GCGGTGTTCA ATGAGGATGG GTCCCTGGCT 
GAGCTGAAGG GCTTTGAGGT GAAGCGCCGG GGCGAGCTGC AGCTGGTGAA GATATTCCAG 
TCTTCTGTAT TCGAGGCCTT CCTGAAAGGC ACCACCCTGG AGGAGGTCTA TGCTTCTGTG 
GCCAAGGTGG CTGACTACTG GCTGGATGTG CTGTACAGCA AGGCTGCCAA CATGCCCGAT 
TCGGAGCTGT TTGAGCTCAT CTCAGAGAAC CGTTCCATGT CTCGCAAGCT GGAGGACTAT 
GGGGAGCAGA AATCCACCTC CATCAGCACT GCCAAGCGCC TGGCAGAGTT CCTGGGGGAC 
CAGATGGTGA AGGATGCAGG GCTGAGCTGC CGCTTCATCA TCTCCAAGAA ACCTGAGGGC 
TCTCCTGTCA CTGAGAGGGC CATCCCCCTT GCCATCTTCC AAGCAGAGCC CAGCGTACGG 
AAGCACTACC TCCGGAAATG GCTGAAGAGC CCCTCGCTGC AGGACTTCAA CATTCGGGCA 
ATTCTGGACT GGGACTACTA CATTGAGAGA CTGGGAAGCA CCATCCAGAA AATCATCACT 
ATTCCTGCAG CCCTGCAGCA GGTAAAGAAC CCAGTGCCAC GGGTCCGACA CCCGGACTGG 
CTGCACAAGA AACTCCTGGA GAAGAATGAT GTGTACAAAC AGAAAAAGAT CAATGAGCTC 
TTCACTTCTG AAGGCAAGAG GCAGGTCCTC GCCAACCAGC CCCAGGAGGG CACACCCAGC 
TCGCAGGTTG GTGACATAGA GGATTTTGGG GCTGCCAAGA GCCTTCATCC ATCCGTTCCC 
ATTGCAAACA AACGGAAGCG AGTCCCTACA GCAGAGGAAA GCCAACAGAT GTCCCAGAGC 
TTGGAGCTGT CCCAGTCCTG GCGGGAGATC CTGGGTCCTC CTCCCTCCAT GGGCACCACC 
AAGGAGGAGC TCGTGGCCTG GCTGCGCTTC CACCAGAAGA AATGGGAGCT GCAGGCCCGG 
CAGCGGCGCG AGCGGCAGAA GAGGCGGCGC CTGGAGGACG GTGGGACGGC GGCGGGCGGC 
GGGGTGGTCC GTGATGCCCT CTCCAGGGGG CTGGGCAGCT ACTTGCGCCA CACTGCGCGC 
AGCATCCTGG ACTTGCCCTG GCAGATCGTG CAGATCGCTG AGACCAGCCA GCCTGGCTTG 
TTCAGGCTAT GGGCGGTGAT CGGGAGCGAC CTGCACTGCA TCAAGCTGAG CATCCCCAGG 
GTGTTCTACG TCAACCAGCG CGTCGCAAAG CCGGAGGAGG GAGCGGTCTA TAGGAAGGTG 
AACAGGATCC TGCCCCGATC AAACCTGGTG TACAACCTGT ACGAATACTC AGTCCCTGAG 
GACATGTACC AGGAGCACAT CAACGAGATC AATGCTGACC TCTCTGCTCC CGACATCGAG 
GGAGTGTATG AGACTCAGGT GCCACTGCTG CTGCGAGCCC TGATCCAGCT GGGCTGTGTC 
TGCATGGTCA GCAGGCAGCT CGTCCGGCAC CTGACGGGCC GTGAGGCGGA CACCTTCGAC 
ATCGAGCACC TGGAGATGCG CTCGCTGGCC CAGTTCCCCT ACCTGGAGCC AGGGAGCATC 
CGCCACATCT ACCTCTACCA TAGCTCCCAG GGCAGCAGGG CCCTCTTCGG CCTCTTCATC 
CCCGCGCAGC GCAAGGCTGC CGTCTTTGTG CTGGACACGG TGCGCAGCAA CCAGATGCCA 
AACCTCACCA CCATGTTCTC AGCAGAGCGC AGCGCGCTGC TGGAAAGGGT GGGTGAGGAG 
CTGCTGCCTC CAGACAAGCA CACCTTCGAA GTGCGCGCCG AGACTGACCC CAAGGCCATC 
TGCAGAGCCA TCCAGCGGCT GCTGCTTGGT TACAAGGACG AGCGCCGGGG TCCCACGCTG 
ATCGCTGTGC AGTCCAACTG GGAGCTGAAG CGGCTGGCGA GCGGTATTCC CATCCTCGAC 
GAGTTCCCCC TGGTCCCCAT CCGGGTGACA GATGACATCA GCTACTCGGT CCTGGACTGG 
CAGCGCCACG CAGCCCGACG CATGATCCGC CATTACCTCA ACCTGGACAC ATGCCTGTCG 
CAGGCCTTTG AGATGAGCAG ATATTACCAC ATTCCCATCG GGAACCTCCC TGATGACATC 
TCCACCTTTG GCTCTGACCT GTTCTTCTCA CGGCACCTCC GTCGTCACAA CCATTTGCTG 
TGGCTCTCAC CCACGGCCCG CCCAGACCTG GGGGGGAAGG AGGCTGATGA CAACCGCCTC 
GTCATGGAGT TCGATGAGAG AGCCTCAGTG GAGATAAACA ACCCCGGCTG CTATTCCACG 
GTGTGTCTGG AGCTGGACAT CCAGAGCCTG GCTGTGAACA CAGTGCTGCA ATCTCATCAT 
GTGAACGATA TGGAAGGATG TTCCAGCATG AGCATCAGCT TCGACGTAAT TCAGCAGGCA 
TCCTTGGAAG ATATGGTGAC AGGGAACCAA GCTGCCAACA TCCCAGCCAG CTATGATGAA 
ACCGCCCTCT GCTCCAACAC ATTCAGGATT CTGAAGAGCA TGGTGGTGAG CTGGGTGAAG 
GAGATCACAC AGTACCACAA CATCTATGCA GACAACCAGG TCATTCACTT CTACCGCTGG 
CTTCGCTCTC CTTCCTCGCT GCTCTACGAC CCGGCGCTGC ACCGCACGTT GCACAACATG 
ATGAAGAAGC TTTTCCTTCA GTTGGTGGCT GAGTTCAGGC GCCTCGGCTC TTCGGTGGTA 
TACGCCAACT TCAACCGGAT CATTCTGTGC ACCAAGAAGC GGCGTATCGA GGACGCCATC 
AGCTACATGG AGTACATTAT CAACAGCATC CACTCAAAGG AGATCTTCCA CTCTCTGACT 
ATTTCCTTCT CCCGCTGCTG GGAGTTCCTG CTCTGGATGG ATCCAGCCAA TTATGGAGGT 
ATCAAGGGAA AAGTGCATTC TCGCATCCAC TGCGGGGAGG ATACCAGCAA GAAGCAAGCA 
CCAGAGGAGG GAGACAGTGA GGAAGAGGAG GAGGCAGGTG AAGATGATGA GGAAGAAGGG 
GAGTCCAATG TGGAGGAGCT GCTGGAGAAC AGCTGGAACA TCGTGCAGTT CCTGCCGCAG 
GCTGCCTCCT GCCAGAACTA CTTCCTGATG ATTGTGTCAG CCTACATCGT GGCCGTGTAC 
CATAGCATGA AGGAGGAGCT GCGGCGCAAT GCCCCCGGCA GCACTCCCGT CAAGAGAAGA 
GGCACCAGCC AGGTGACCCA GGAGGCACAG GGCGAGACAG GAGCCATGCC TGGGATGATC 
ACCTTCTCAC AGGATTACGT GGCCAATGAG CTCACTCAGA GCTTCTTCAC CATCACACAG 
AAGATCCAGA AGAAGATGTC TGGCTCCCGA CACACCACTG AGCCATCAGA CTTGTTCCCG 
GTGCTGCCCG GCTCCTACTT GCCGCTCAAC AACCCTGCTC TGGAGTTCAT CAAATACGTT 
TGCCAGGTGC TGTCCCTGGA TGCCAACGTC ACCAACCAGG TGAACAAGCT GCGCAGGGAC 
CTGCTGCGCC TCATCGAGGT GGGCGAGTTC TCAGATGAAG CCCAGTTTCG GGACCCTTGC 
CGCTCCTATG TGCTCCCTGA AGTCATCTGC AGAAACTGCA ACTTCTGCAG GGACCTGGAC 
CTGTGCAAGG ACCCAGCCCT CTCCCAGGAC GGATCGCTGC TGCCCAGCTG GGTGTGCTCC 
AACTGCCAGG CACAGTACGA CTCCGACTCC ATAGAAATGG CCTTGGTGGA AGCCCTGCAG 
AAGAAGCTGA TGGCCTTCAT GCTGCAGGAC CTGGTGTGCA AGAAGTGCCA CGGTGTGAAG 
GACACACACA TGCCTTTGTA CTGCAGCTGT GCTGGAGACT TTGCCCTCAC CATCTCCTCC 
CAGACCTTCA TGGAGCACAT CACCGTCTTC CAGAACATCG CCAGGCACTA CGGCATGGCC 
CATCTGCTGG AAACCATCGA ATGGCTGCTG CACACCAACC CCCAGCTCCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001233985.3
CDS	62..6898
Translation	MVLRGGGGGDRDPEGTRDDGPALSALKRLERSQRTDQLDALFGFERPAEPCERTGWLINMHPTEVLDEDRRAVSAVDYYFIQEDGSRFKVALPYRPYFYVATQQGCEREVSSFLAKKFQGKIAKLETVPKEDLDLPNHLVGLKRHYLKLSFSTVDELVKVRKEISPAVRKNRERDQANDVYTSMLSSALAGGSLTTSEEGASKKVTNQMDNIVDMREYDVPYHMRLSIDLKIHVAHWYNVRYRGSTFPPEITRRDDLVERPDPVVLAFDIETTKLPLKFPDPETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIIVTYNGDFFDWPFVEARAAAHGINMYQEIGFQKDSQGEYKASQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEEMCRMATEEPQTLATYSVSDAVATYYMYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAYHANIIFPNKQEQEFNKLTEDGHVLDSETYVGGHVEALESGVFRSDIPCRFKMNPAAFDYLVQNVEKTLRHAIEEEERLPLDQVTNFQEVCDEIKVKLNSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRRMTWQWRGEFMSASRSEYHRIQQQLESEKFPPLYPDGAPRAFHELSREEQAKYEKKRLADYCRKAYKKIHVTKVEERVTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAMETGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKSTNAKKPKVTISYPGAMLNILVKEGFTNDQYQELQDPASLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLVKIFQSSVFEAFLKGTTLEEVYASVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRFIISKKPEGSPVTERAIPLAIFQAEPSVRKHYLRKWLKSPSLQDFNIRAILDWDYYIERLGSTIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLEKNDVYKQKKINELFTSEGKRQVLANQPQEGTPSSQVGDIEDFGAAKSLHPSVPIANKRKRVPTAEESQQMSQSLELSQSWREILGPPPSMGTTKEELVAWLRFHQKKWELQARQRRERQKRRRLEDGGTAAGGGVVRDALSRGLGSYLRHTARSILDLPWQIVQIAETSQPGLFRLWAVIGSDLHCIKLSIPRVFYVNQRVAKPEEGAVYRKVNRILPRSNLVYNLYEYSVPEDMYQEHINEINADLSAPDIEGVYETQVPLLLRALIQLGCVCMVSRQLVRHLTGREADTFDIEHLEMRSLAQFPYLEPGSIRHIYLYHSSQGSRALFGLFIPAQRKAAVFVLDTVRSNQMPNLTTMFSAERSALLERVGEELLPPDKHTFEVRAETDPKAICRAIQRLLLGYKDERRGPTLIAVQSNWELKRLASGIPILDEFPLVPIRVTDDISYSVLDWQRHAARRMIRHYLNLDTCLSQAFEMSRYYHIPIGNLPDDISTFGSDLFFSRHLRRHNHLLWLSPTARPDLGGKEADDNRLVMEFDERASVEINNPGCYSTVCLELDIQSLAVNTVLQSHHVNDMEGCSSMSISFDVIQQASLEDMVTGNQAANIPASYDETALCSNTFRILKSMVVSWVKEITQYHNIYADNQVIHFYRWLRSPSSLLYDPALHRTLHNMMKKLFLQLVAEFRRLGSSVVYANFNRIILCTKKRRIEDAISYMEYIINSIHSKEIFHSLTISFSRCWEFLLWMDPANYGGIKGKVHSRIHCGEDTSKKQAPEEGDSEEEEEAGEDDEEEGESNVEELLENSWNIVQFLPQAASCQNYFLMIVSAYIVAVYHSMKEELRRNAPGSTPVKRRGTSQVTQEAQGETGAMPGMITFSQDYVANELTQSFFTITQKIQKKMSGSRHTTEPSDLFPVLPGSYLPLNNPALEFIKYVCQVLSLDANVTNQVNKLRRDLLRLIEVGEFSDEAQFRDPCRSYVLPEVICRNCNFCRDLDLCKDPALSQDGSLLPSWVCSNCQAQYDSDSIEMALVEALQKKLMAFMLQDLVCKKCHGVKDTHMPLYCSCAGDFALTISSQTFMEHITVFQNIARHYGMAHLLETIEWLLHTNPQLQQ

Target ORF information:

RefSeq Version	XM_001233984.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus polymerase (DNA directed), epsilon, catalytic subunit (POLE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001233984.4

ORF Insert Sequence:

ATGGTGCTGC GGGGCGGCGG CGGCGGCGAC CGTGACCCCG AGGGCACCCG GGATGATGGC 
CCCGCGCTGT CTGCCCTCAA GCGCCTGGAG CGGTCGCAGC GCACGGACCA GTTGGACGCG 
CTGTTCGGCT TCGAGCGACC CGCCGAGCCC TGCGAGCGGA CGGGCTGGCT CATCAACATG 
CACCCGACCG AGGTGCTGGA TGAGGACCGG CGGGCAGTGA GCGCTGTGGA CTATTACTTC 
ATCCAGGAGG ATGGCAGCCG CTTCAAGGTG GCCCTGCCCT ACAGACCCTA CTTCTATGTG 
GCCACCCAGC AGGGCTGCGA GCGGGAGGTG TCCTCCTTCC TGGCCAAGAA GTTCCAAGGG 
AAGATTGCGA AGCTGGAAAC TGTCCCAAAG GAAGATTTGG ATCTGCCCAA CCACCTGGTG 
GGGCTGAAGC GGCACTACCT CAAGCTGTCC TTCAGCACAG TGGATGAGTT GGTGAAGGTG 
AGGAAAGAGA TCTCCCCTGC TGTAAGGAAG AACAGGGAGC GCGACCAGGC CAATGATGTG 
TACACATCCA TGCTGTCAAG TGCTCTGGCT GGTGGCAGCC TGACCACATC GGAGGAGGGA 
GCCTCCAAAA AGGTCACCAA TCAAATGGAC AACATTGTGG ACATGCGGGA GTACGACGTG 
CCCTACCACA TGCGTCTCTC CATCGACCTC AAGATCCACG TGGCTCACTG GTACAACGTG 
CGGTACCGCG GCAGCACCTT CCCCCCCGAG ATCACCCGCA GGGATGACCT GGTGGAGCGG 
CCGGATCCTG TTGTCCTCGC CTTTGACATT GAAACCACAA AACTCCCCTT GAAGTTTCCT 
GATCCAGAGA CGGACCAGAT CATGATGATC TCCTACATGA TTGATGGGCA GGGCTACCTG 
ATCACAAATA GGGAGATCGT CTCTGAGGAT ATCGAAGACT TTGAGTTCAC TCCCAAGCCG 
GAGTACGAGG GTCCCTTTTG TGTCTTTAAC GAGCCAGATG AAGCTCATTT AATCCAGAGA 
TGGTTTGAAC ACGTCCAGGA GACCAAACCT ACCATCATTG TCACATACAA TGGAGACTTC 
TTTGACTGGC CCTTTGTAGA AGCAAGAGCC GCAGCTCATG GAATTAACAT GTACCAGGAA 
ATTGGATTTC AGAAGGACAG CCAGGGAGAG TACAAAGCAT CACAGTGCAT CCACATGGAC 
TGCCTAAGGT GGGTGAAGAG AGACAGCTAC CTCCCAGTGG GAAGTCACAA CCTGAAGGCA 
GCAGCTAAAG CAAAACTGGG CTATGACCCA GTGGAGCTGG ACCCTGAAGA GATGTGCAGG 
ATGGCTACAG AGGAGCCTCA GACCCTGGCC ACCTATTCAG TGTCCGATGC TGTTGCCACC 
TATTACATGT ATATGAAGTA TGTGCACCCT TTCATTTTTG CCTTGTGCAC CATCATTCCC 
ATGGAGCCCG ATGAGGTGTT ACGAAAAGGT TCTGGGACGC TGTGCGAGGC GCTGCTGATG 
GTTCAAGCCT ATCATGCCAA CATCATCTTC CCCAACAAGC AGGAGCAGGA ATTCAACAAG 
CTCACAGAGG ATGGCCACGT GCTGGACTCG GAGACGTACG TTGGAGGCCA CGTGGAAGCA 
CTGGAGTCGG GGGTGTTCCG CAGTGACATC CCCTGCCGCT TCAAAATGAA CCCTGCTGCC 
TTTGACTACT TGGTGCAGAA CGTGGAGAAG ACGCTCCGGC ACGCTATTGA GGAGGAGGAG 
CGTTTACCGT TGGATCAAGT CACCAACTTC CAGGAGGTCT GTGATGAAAT CAAAGTCAAA 
CTGAATTCCC TGAAGGATGT TCCTAACCGA ATTGAGTGTC CCCTTATCTA CCATTTAGAT 
GTGGGAGCCA TGTACCCCAA CATCATTCTG ACCAACAGAT TGCAGCCCTC AGCAATGGTG 
GATGAGGCCA CATGTGCTGC CTGTGACTTC AACAAGCCAG GGGCCAACTG CCAGCGCCGG 
ATGACGTGGC AATGGAGAGG AGAATTCATG TCTGCGAGCC GCAGTGAGTA CCACCGCATC 
CAGCAGCAAT TGGAGTCGGA GAAGTTCCCT CCACTGTACC CTGATGGAGC ACCCCGTGCT 
TTCCACGAGC TCTCCCGAGA AGAACAAGCC AAGTATGAGA AGAAACGGCT GGCAGATTAC 
TGTCGCAAAG CCTACAAGAA GATCCATGTC ACCAAGGTGG AGGAGAGGGT CACCACCATC 
TGCCAACGAG AGAACTCTTT CTATGTGGAC ACGGTGCGAG CTTTCAGGGA CCGCCGCTAT 
GAGTTCAAGG GGCTGCACAA GGTATGGAAG AAGAAGCTGT CTGCAGCCAT GGAGACAGGA 
GATGCCTCTG AAGTGAAACG CTGCAAGAAC ATGGAGATCC TCTATGACTC CCTGCAGTTG 
GCACACAAGT GCATTCTCAA TTCTTTCTAT GGCTATGTCA TGCGGAAGGG AGCTCGATGG 
TACTCCATGG AAATGGCCGG GATTGTCTGC TTCACCGGAG CCAATATCAT CACACAGGCC 
AGGGAACTGA TTGAGCAGAT TGGGAGACCT CTGGAACTGG ATACAGATGG GATTTGGTGT 
GTCCTCCCCA ACAGCTTCCC AGAAAACTTT GTCATCAAGT CAACCAATGC CAAGAAGCCA 
AAGGTGACAA TCTCCTACCC AGGTGCTATG CTGAACATCC TGGTGAAGGA AGGCTTCACA 
AACGATCAGT ACCAGGAGCT GCAGGACCCA GCCTCTCTCA CCTACGTCAC ACGCTCGGAG 
AACAGCATCT TCTTTGAAGT GGATGGGCCA TACCTGGCCA TGATTCTCCC AGCTTCCAAG 
GAGGAGGGAA AGAAGCTGAA GAAAAGGTAT GCGGTGTTCA ATGAGGATGG GTCCCTGGCT 
GAGCTGAAGG GCTTTGAGGT GAAGCGCCGG GGCGAGCTGC AGCTGGTGAA GATATTCCAG 
TCTTCTGTAT TCGAGGCCTT CCTGAAAGGC ACCACCCTGG AGGAGGTCTA TGCTTCTGTG 
GCCAAGGTGG CTGACTACTG GCTGGATGTG CTGTACAGCA AGGCTGCCAA CATGCCCGAT 
TCGGAGCTGT TTGAGCTCAT CTCAGAGAAC CGTTCCATGT CTCGCAAGCT GGAGGACTAT 
GGGGAGCAGA AATCCACCTC CATCAGCACT GCCAAGCGCC TGGCAGAGTT CCTGGGGGAC 
CAGATGGTGA AGGATGCAGG GCTGAGCTGC CGCTTCATCA TCTCCAAGAA ACCTGAGGGC 
TCTCCTGTCA CTGAGAGGGC CATCCCCCTT GCCATCTTCC AAGCAGAGCC CAGCGTACGG 
AAGCACTACC TCCGGAAATG GCTGAAGAGC CCCTCGCTGC AGGACTTCAA CATTCGGGCA 
ATTCTGGACT GGGACTACTA CATTGAGAGA CTGGGAAGCA CCATCCAGAA AATCATCACT 
ATTCCTGCAG CCCTGCAGCA GGTAAAGAAC CCAGTGCCAC GGGTCCGACA CCCGGACTGG 
CTGCACAAGA AACTCCTGGA GAAGAATGAT GTGTACAAAC AGAAAAAGAT CAATGAGCTC 
TTCACTTCTG AAGGCAAGAG GCAGGTCCTC GCCAACCAGC CCCAGGAGGG CACACCCAGC 
TCGCAGGTTG GTGACATAGA GGATTTTGGG GCTGCCAAGA GCCTTCATCC ATCCGTTCCC 
ATTGCAAACA AACGGAAGCG AGTCCCTACA GCAGAGGAAA GCCAACAGAT GTCCCAGAGC 
TTGGAGCTGT CCCAGTCCTG GCGGGAGATC CTGGGTCCTC CTCCCTCCAT GGGCACCACC 
AAGGAGGAGC TCGTGGCCTG GCTGCGCTTC CACCAGAAGA AATGGGAGCT GCAGGCCCGG 
CAGCGGCGCG AGCGGCAGAA GAGGCGGCGC CTGGAGGACG GTGGGACGGC GGCGGGCGGC 
GGGGTGGTCC GTGATGCCCT CTCCAGGGGG CTGGGCAGCT ACTTGCGCCA CACTGCGCGC 
AGCATCCTGG ACTTGCCCTG GCAGATCGTG CAGATCGCTG AGACCAGCCA GCCTGGCTTG 
TTCAGGCTAT GGGCGGTGAT CGGGAGCGAC CTGCACTGCA TCAAGCTGAG CATCCCCAGG 
GTGTTCTACG TCAACCAGCG CGTCGCAAAG CCGGAGGAGG GAGCGGTCTA TAGGAAGGTG 
AACAGGATCC TGCCCCGATC AAACCTGGTG TACAACCTGT ACGAATACTC AGTCCCTGAG 
GACATGTACC AGGAGCACAT CAACGAGATC AATGCTGACC TCTCTGCTCC CGACATCGAG 
GGAGTGTATG AGACTCAGGT GCCACTGCTG CTGCGAGCCC TGATCCAGCT GGGCTGTGTC 
TGCATGGTCA GCAGGCAGCT CGTCCGGCAC CTGACGGGCC GTGAGGCGGA CACCTTCGAC 
ATCGAGCACC TGGAGATGCG CTCGCTGGCC CAGTTCCCCT ACCTGGAGCC AGGGAGCATC 
CGCCACATCT ACCTCTACCA TAGCTCCCAG GGCAGCAGGG CCCTCTTCGG CCTCTTCATC 
CCCGCGCAGC GCAAGGCTGC CGTCTTTGTG CTGGACACGG TGCGCAGCAA CCAGATGCCA 
AACCTCACCA CCATGTTCTC AGCAGAGCGC AGCGCGCTGC TGGAAAGGGT GGGTGAGGAG 
CTGCTGCCTC CAGACAAGCA CACCTTCGAA GTGCGCGCCG AGACTGACCC CAAGGCCATC 
TGCAGAGCCA TCCAGCGGCT GCTGCTTGGT TACAAGGACG AGCGCCGGGG TCCCACGCTG 
ATCGCTGTGC AGTCCAACTG GGAGCTGAAG CGGCTGGCGA GCGGTATTCC CATCCTCGAC 
GAGTTCCCCC TGGTCCCCAT CCGGGTGACA GATGACATCA GCTACTCGGT CCTGGACTGG 
CAGCGCCACG CAGCCCGACG CATGATCCGC CATTACCTCA ACCTGGACAC ATGCCTGTCG 
CAGGCCTTTG AGATGAGCAG ATATTACCAC ATTCCCATCG GGAACCTCCC TGATGACATC 
TCCACCTTTG GCTCTGACCT GTTCTTCTCA CGGCACCTCC GTCGTCACAA CCATTTGCTG 
TGGCTCTCAC CCACGGCCCG CCCAGACCTG GGGGGGAAGG AGGCTGATGA CAACCGCCTC 
GTCATGGAGT TCGATGAGAG AGCCTCAGTG GAGATAAACA ACCCCGGCTG CTATTCCACG 
GTGTGTCTGG AGCTGGACAT CCAGAGCCTG GCTGTGAACA CAGTGCTGCA ATCTCATCAT 
GTGAACGATA TGGAAGGATG TTCCAGCATG AGCATCAGCT TCGACGTAAT TCAGCAGGCA 
TCCTTGGAAG ATATGGTGAC AGGGAACCAA GCTGCCAACA TCCCAGCCAG CTATGATGAA 
ACCGCCCTCT GCTCCAACAC ATTCAGGATT CTGAAGAGCA TGGTGGTGAG CTGGGTGAAG 
GAGATCACAC AGTACCACAA CATCTATGCA GACAACCAGG TCATTCACTT CTACCGCTGG 
CTTCGCTCTC CTTCCTCGCT GCTCTACGAC CCGGCGCTGC ACCGCACGTT GCACAACATG 
ATGAAGAAGC TTTTCCTTCA GTTGGTGGCT GAGTTCAGGC GCCTCGGCTC TTCGGTGGTA 
TACGCCAACT TCAACCGGAT CATTCTGTGC ACCAAGAAGC GGCGTATCGA GGACGCCATC 
AGCTACATGG AGTACATTAT CAACAGCATC CACTCAAAGG AGATCTTCCA CTCTCTGACT 
ATTTCCTTCT CCCGCTGCTG GGAGTTCCTG CTCTGGATGG ATCCAGCCAA TTATGGAGGT 
ATCAAGGGAA AAGTGCATTC TCGCATCCAC TGCGGGGAGG ATACCAGCAA GAAGCAAGCA 
CCAGAGGAGG GAGACAGTGA GGAAGAGGAG GAGGCAGGTG AAGATGATGA GGAAGAAGGG 
GAGTCCAATG TGGAGGAGCT GCTGGAGAAC AGCTGGAACA TCGTGCAGTT CCTGCCGCAG 
GCTGCCTCCT GCCAGAACTA CTTCCTGATG ATTGTGTCAG CCTACATCGT GGCCGTGTAC 
CATAGCATGA AGGAGGAGCT GCGGCGCAAT GCCCCCGGCA GCACTCCCGT CAAGAGAAGA 
GGCACCAGCC AGGTGACCCA GGAGGCACAG GGCGAGACAG GAGCCATGCC TGGGATGATC 
ACCTTCTCAC AGGATTACGT GGCCAATGAG CTCACTCAGA GCTTCTTCAC CATCACACAG 
AAGATCCAGA AGAAGATGTC TGGCTCCCGA CACACCACTG AGCCATCAGA CTTGTTCCCG 
GTGCTGCCCG GCTCCTACTT GCCGCTCAAC AACCCTGCTC TGGAGTTCAT CAAATACGTT 
TGCCAGGTGC TGTCCCTGGA TGCCAACGTC ACCAACCAGG TGAACAAGCT GCGCAGGGAC 
CTGCTGCGCC TCATCGAGGT GGGCGAGTTC TCAGATGAAG CCCAGTTTCG GGACCCTTGC 
CGCTCCTATG TGCTCCCTGA AGTCATCTGC AGAAACTGCA ACTTCTGCAG GGACCTGGAC 
CTGTGCAAGG ACCCAGCCCT CTCCCAGGAC GGATCGCTGC TGCCCAGCTG GGTGTGCTCC 
AACTGCCAGG CACAGTACGA CTCCGACTCC ATAGAAATGG CCTTGGTGGA AGCCCTGCAG 
AAGAAGCTGA TGGCCTTCAT GCTGCAGGAC CTGGTGTGCA AGAAGTGCCA CGGTGTGAAG 
GACACACACA TGCCTTTGTA CTGCAGCTGT GCTGGAGACT TTGCCCTCAC CATCTCCTCC 
CAGACCTTCA TGGAGCACAT CACCGTCTTC CAGAACATCG CCAGGCACTA CGGCATGGCC 
CATCTGCTGG AAACCATCGA ATGGCTGCTG CACACCAACC CCCAGCTCCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_001233984.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_001233985.3 Gallus gallus DNA polymerase epsilon, catalytic subunit (POLE), mRNA.

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CloneID	OGa15806
Clone ID Related Accession (Same CDS sequence)	XM_001233984.4 , XM_001233984.5
Accession Version	XM_001233984.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	DNA polymerase epsilon catalytic subunit A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_001233984.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTGC GGGGCGGCGG CGGCGGCGAC CGTGACCCCG AGGGCACCCG GGATGATGGC 
CCCGCGCTGT CTGCCCTCAA GCGCCTGGAG CGGTCGCAGC GCACGGACCA GTTGGACGCG 
CTGTTCGGCT TCGAGCGACC CGCCGAGCCC TGCGAGCGGA CGGGCTGGCT CATCAACATG 
CACCCGACCG AGGTGCTGGA TGAGGACCGG CGGGCAGTGA GCGCTGTGGA CTATTACTTC 
ATCCAGGAGG ATGGCAGCCG CTTCAAGGTG GCCCTGCCCT ACAGACCCTA CTTCTATGTG 
GCCACCCAGC AGGGCTGCGA GCGGGAGGTG TCCTCCTTCC TGGCCAAGAA GTTCCAAGGG 
AAGATTGCGA AGCTGGAAAC TGTCCCAAAG GAAGATTTGG ATCTGCCCAA CCACCTGGTG 
GGGCTGAAGC GGCACTACCT CAAGCTGTCC TTCAGCACAG TGGATGAGTT GGTGAAGGTG 
AGGAAAGAGA TCTCCCCTGC TGTAAGGAAG AACAGGGAGC GCGACCAGGC CAATGATGTG 
TACACATCCA TGCTGTCAAG TGCTCTGGCT GGTGGCAGCC TGACCACATC GGAGGAGGGA 
GCCTCCAAAA AGGTCACCAA TCAAATGGAC AACATTGTGG ACATGCGGGA GTACGACGTG 
CCCTACCACA TGCGTCTCTC CATCGACCTC AAGATCCACG TGGCTCACTG GTACAACGTG 
CGGTACCGCG GCAGCACCTT CCCCCCCGAG ATCACCCGCA GGGATGACCT GGTGGAGCGG 
CCGGATCCTG TTGTCCTCGC CTTTGACATT GAAACCACAA AACTCCCCTT GAAGTTTCCT 
GATCCAGAGA CGGACCAGAT CATGATGATC TCCTACATGA TTGATGGGCA GGGCTACCTG 
ATCACAAATA GGGAGATCGT CTCTGAGGAT ATCGAAGACT TTGAGTTCAC TCCCAAGCCG 
GAGTACGAGG GTCCCTTTTG TGTCTTTAAC GAGCCAGATG AAGCTCATTT AATCCAGAGA 
TGGTTTGAAC ACGTCCAGGA GACCAAACCT ACCATCATTG TCACATACAA TGGAGACTTC 
TTTGACTGGC CCTTTGTAGA AGCAAGAGCC GCAGCTCATG GAATTAACAT GTACCAGGAA 
ATTGGATTTC AGAAGGACAG CCAGGGAGAG TACAAAGCAT CACAGTGCAT CCACATGGAC 
TGCCTAAGGT GGGTGAAGAG AGACAGCTAC CTCCCAGTGG GAAGTCACAA CCTGAAGGCA 
GCAGCTAAAG CAAAACTGGG CTATGACCCA GTGGAGCTGG ACCCTGAAGA GATGTGCAGG 
ATGGCTACAG AGGAGCCTCA GACCCTGGCC ACCTATTCAG TGTCCGATGC TGTTGCCACC 
TATTACATGT ATATGAAGTA TGTGCACCCT TTCATTTTTG CCTTGTGCAC CATCATTCCC 
ATGGAGCCCG ATGAGGTGTT ACGAAAAGGT TCTGGGACGC TGTGCGAGGC GCTGCTGATG 
GTTCAAGCCT ATCATGCCAA CATCATCTTC CCCAACAAGC AGGAGCAGGA ATTCAACAAG 
CTCACAGAGG ATGGCCACGT GCTGGACTCG GAGACGTACG TTGGAGGCCA CGTGGAAGCA 
CTGGAGTCGG GGGTGTTCCG CAGTGACATC CCCTGCCGCT TCAAAATGAA CCCTGCTGCC 
TTTGACTACT TGGTGCAGAA CGTGGAGAAG ACGCTCCGGC ACGCTATTGA GGAGGAGGAG 
CGTTTACCGT TGGATCAAGT CACCAACTTC CAGGAGGTCT GTGATGAAAT CAAAGTCAAA 
CTGAATTCCC TGAAGGATGT TCCTAACCGA ATTGAGTGTC CCCTTATCTA CCATTTAGAT 
GTGGGAGCCA TGTACCCCAA CATCATTCTG ACCAACAGAT TGCAGCCCTC AGCAATGGTG 
GATGAGGCCA CATGTGCTGC CTGTGACTTC AACAAGCCAG GGGCCAACTG CCAGCGCCGG 
ATGACGTGGC AATGGAGAGG AGAATTCATG TCTGCGAGCC GCAGTGAGTA CCACCGCATC 
CAGCAGCAAT TGGAGTCGGA GAAGTTCCCT CCACTGTACC CTGATGGAGC ACCCCGTGCT 
TTCCACGAGC TCTCCCGAGA AGAACAAGCC AAGTATGAGA AGAAACGGCT GGCAGATTAC 
TGTCGCAAAG CCTACAAGAA GATCCATGTC ACCAAGGTGG AGGAGAGGGT CACCACCATC 
TGCCAACGAG AGAACTCTTT CTATGTGGAC ACGGTGCGAG CTTTCAGGGA CCGCCGCTAT 
GAGTTCAAGG GGCTGCACAA GGTATGGAAG AAGAAGCTGT CTGCAGCCAT GGAGACAGGA 
GATGCCTCTG AAGTGAAACG CTGCAAGAAC ATGGAGATCC TCTATGACTC CCTGCAGTTG 
GCACACAAGT GCATTCTCAA TTCTTTCTAT GGCTATGTCA TGCGGAAGGG AGCTCGATGG 
TACTCCATGG AAATGGCCGG GATTGTCTGC TTCACCGGAG CCAATATCAT CACACAGGCC 
AGGGAACTGA TTGAGCAGAT TGGGAGACCT CTGGAACTGG ATACAGATGG GATTTGGTGT 
GTCCTCCCCA ACAGCTTCCC AGAAAACTTT GTCATCAAGT CAACCAATGC CAAGAAGCCA 
AAGGTGACAA TCTCCTACCC AGGTGCTATG CTGAACATCC TGGTGAAGGA AGGCTTCACA 
AACGATCAGT ACCAGGAGCT GCAGGACCCA GCCTCTCTCA CCTACGTCAC ACGCTCGGAG 
AACAGCATCT TCTTTGAAGT GGATGGGCCA TACCTGGCCA TGATTCTCCC AGCTTCCAAG 
GAGGAGGGAA AGAAGCTGAA GAAAAGGTAT GCGGTGTTCA ATGAGGATGG GTCCCTGGCT 
GAGCTGAAGG GCTTTGAGGT GAAGCGCCGG GGCGAGCTGC AGCTGGTGAA GATATTCCAG 
TCTTCTGTAT TCGAGGCCTT CCTGAAAGGC ACCACCCTGG AGGAGGTCTA TGCTTCTGTG 
GCCAAGGTGG CTGACTACTG GCTGGATGTG CTGTACAGCA AGGCTGCCAA CATGCCCGAT 
TCGGAGCTGT TTGAGCTCAT CTCAGAGAAC CGTTCCATGT CTCGCAAGCT GGAGGACTAT 
GGGGAGCAGA AATCCACCTC CATCAGCACT GCCAAGCGCC TGGCAGAGTT CCTGGGGGAC 
CAGATGGTGA AGGATGCAGG GCTGAGCTGC CGCTTCATCA TCTCCAAGAA ACCTGAGGGC 
TCTCCTGTCA CTGAGAGGGC CATCCCCCTT GCCATCTTCC AAGCAGAGCC CAGCGTACGG 
AAGCACTACC TCCGGAAATG GCTGAAGAGC CCCTCGCTGC AGGACTTCAA CATTCGGGCA 
ATTCTGGACT GGGACTACTA CATTGAGAGA CTGGGAAGCA CCATCCAGAA AATCATCACT 
ATTCCTGCAG CCCTGCAGCA GGTAAAGAAC CCAGTGCCAC GGGTCCGACA CCCGGACTGG 
CTGCACAAGA AACTCCTGGA GAAGAATGAT GTGTACAAAC AGAAAAAGAT CAATGAGCTC 
TTCACTTCTG AAGGCAAGAG GCAGGTCCTC GCCAACCAGC CCCAGGAGGG CACACCCAGC 
TCGCAGGTTG GTGACATAGA GGATTTTGGG GCTGCCAAGA GCCTTCATCC ATCCGTTCCC 
ATTGCAAACA AACGGAAGCG AGTCCCTACA GCAGAGGAAA GCCAACAGAT GTCCCAGAGC 
TTGGAGCTGT CCCAGTCCTG GCGGGAGATC CTGGGTCCTC CTCCCTCCAT GGGCACCACC 
AAGGAGGAGC TCGTGGCCTG GCTGCGCTTC CACCAGAAGA AATGGGAGCT GCAGGCCCGG 
CAGCGGCGCG AGCGGCAGAA GAGGCGGCGC CTGGAGGACG GTGGGACGGC GGCGGGCGGC 
GGGGTGGTCC GTGATGCCCT CTCCAGGGGG CTGGGCAGCT ACTTGCGCCA CACTGCGCGC 
AGCATCCTGG ACTTGCCCTG GCAGATCGTG CAGATCGCTG AGACCAGCCA GCCTGGCTTG 
TTCAGGCTAT GGGCGGTGAT CGGGAGCGAC CTGCACTGCA TCAAGCTGAG CATCCCCAGG 
GTGTTCTACG TCAACCAGCG CGTCGCAAAG CCGGAGGAGG GAGCGGTCTA TAGGAAGGTG 
AACAGGATCC TGCCCCGATC AAACCTGGTG TACAACCTGT ACGAATACTC AGTCCCTGAG 
GACATGTACC AGGAGCACAT CAACGAGATC AATGCTGACC TCTCTGCTCC CGACATCGAG 
GGAGTGTATG AGACTCAGGT GCCACTGCTG CTGCGAGCCC TGATCCAGCT GGGCTGTGTC 
TGCATGGTCA GCAGGCAGCT CGTCCGGCAC CTGACGGGCC GTGAGGCGGA CACCTTCGAC 
ATCGAGCACC TGGAGATGCG CTCGCTGGCC CAGTTCCCCT ACCTGGAGCC AGGGAGCATC 
CGCCACATCT ACCTCTACCA TAGCTCCCAG GGCAGCAGGG CCCTCTTCGG CCTCTTCATC 
CCCGCGCAGC GCAAGGCTGC CGTCTTTGTG CTGGACACGG TGCGCAGCAA CCAGATGCCA 
AACCTCACCA CCATGTTCTC AGCAGAGCGC AGCGCGCTGC TGGAAAGGGT GGGTGAGGAG 
CTGCTGCCTC CAGACAAGCA CACCTTCGAA GTGCGCGCCG AGACTGACCC CAAGGCCATC 
TGCAGAGCCA TCCAGCGGCT GCTGCTTGGT TACAAGGACG AGCGCCGGGG TCCCACGCTG 
ATCGCTGTGC AGTCCAACTG GGAGCTGAAG CGGCTGGCGA GCGGTATTCC CATCCTCGAC 
GAGTTCCCCC TGGTCCCCAT CCGGGTGACA GATGACATCA GCTACTCGGT CCTGGACTGG 
CAGCGCCACG CAGCCCGACG CATGATCCGC CATTACCTCA ACCTGGACAC ATGCCTGTCG 
CAGGCCTTTG AGATGAGCAG ATATTACCAC ATTCCCATCG GGAACCTCCC TGATGACATC 
TCCACCTTTG GCTCTGACCT GTTCTTCTCA CGGCACCTCC GTCGTCACAA CCATTTGCTG 
TGGCTCTCAC CCACGGCCCG CCCAGACCTG GGGGGGAAGG AGGCTGATGA CAACCGCCTC 
GTCATGGAGT TCGATGAGAG AGCCTCAGTG GAGATAAACA ACCCCGGCTG CTATTCCACG 
GTGTGTCTGG AGCTGGACAT CCAGAGCCTG GCTGTGAACA CAGTGCTGCA ATCTCATCAT 
GTGAACGATA TGGAAGGATG TTCCAGCATG AGCATCAGCT TCGACGTAAT TCAGCAGGCA 
TCCTTGGAAG ATATGGTGAC AGGGAACCAA GCTGCCAACA TCCCAGCCAG CTATGATGAA 
ACCGCCCTCT GCTCCAACAC ATTCAGGATT CTGAAGAGCA TGGTGGTGAG CTGGGTGAAG 
GAGATCACAC AGTACCACAA CATCTATGCA GACAACCAGG TCATTCACTT CTACCGCTGG 
CTTCGCTCTC CTTCCTCGCT GCTCTACGAC CCGGCGCTGC ACCGCACGTT GCACAACATG 
ATGAAGAAGC TTTTCCTTCA GTTGGTGGCT GAGTTCAGGC GCCTCGGCTC TTCGGTGGTA 
TACGCCAACT TCAACCGGAT CATTCTGTGC ACCAAGAAGC GGCGTATCGA GGACGCCATC 
AGCTACATGG AGTACATTAT CAACAGCATC CACTCAAAGG AGATCTTCCA CTCTCTGACT 
ATTTCCTTCT CCCGCTGCTG GGAGTTCCTG CTCTGGATGG ATCCAGCCAA TTATGGAGGT 
ATCAAGGGAA AAGTGCATTC TCGCATCCAC TGCGGGGAGG ATACCAGCAA GAAGCAAGCA 
CCAGAGGAGG GAGACAGTGA GGAAGAGGAG GAGGCAGGTG AAGATGATGA GGAAGAAGGG 
GAGTCCAATG TGGAGGAGCT GCTGGAGAAC AGCTGGAACA TCGTGCAGTT CCTGCCGCAG 
GCTGCCTCCT GCCAGAACTA CTTCCTGATG ATTGTGTCAG CCTACATCGT GGCCGTGTAC 
CATAGCATGA AGGAGGAGCT GCGGCGCAAT GCCCCCGGCA GCACTCCCGT CAAGAGAAGA 
GGCACCAGCC AGGTGACCCA GGAGGCACAG GGCGAGACAG GAGCCATGCC TGGGATGATC 
ACCTTCTCAC AGGATTACGT GGCCAATGAG CTCACTCAGA GCTTCTTCAC CATCACACAG 
AAGATCCAGA AGAAGATGTC TGGCTCCCGA CACACCACTG AGCCATCAGA CTTGTTCCCG 
GTGCTGCCCG GCTCCTACTT GCCGCTCAAC AACCCTGCTC TGGAGTTCAT CAAATACGTT 
TGCCAGGTGC TGTCCCTGGA TGCCAACGTC ACCAACCAGG TGAACAAGCT GCGCAGGGAC 
CTGCTGCGCC TCATCGAGGT GGGCGAGTTC TCAGATGAAG CCCAGTTTCG GGACCCTTGC 
CGCTCCTATG TGCTCCCTGA AGTCATCTGC AGAAACTGCA ACTTCTGCAG GGACCTGGAC 
CTGTGCAAGG ACCCAGCCCT CTCCCAGGAC GGATCGCTGC TGCCCAGCTG GGTGTGCTCC 
AACTGCCAGG CACAGTACGA CTCCGACTCC ATAGAAATGG CCTTGGTGGA AGCCCTGCAG 
AAGAAGCTGA TGGCCTTCAT GCTGCAGGAC CTGGTGTGCA AGAAGTGCCA CGGTGTGAAG 
GACACACACA TGCCTTTGTA CTGCAGCTGT GCTGGAGACT TTGCCCTCAC CATCTCCTCC 
CAGACCTTCA TGGAGCACAT CACCGTCTTC CAGAACATCG CCAGGCACTA CGGCATGGCC 
CATCTGCTGG AAACCATCGA ATGGCTGCTG CACACCAACC CCCAGCTCCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001233985.3
CDS	108..6944
Translation	MVLRGGGGGDRDPEGTRDDGPALSALKRLERSQRTDQLDALFGFERPAEPCERTGWLINMHPTEVLDEDRRAVSAVDYYFIQEDGSRFKVALPYRPYFYVATQQGCEREVSSFLAKKFQGKIAKLETVPKEDLDLPNHLVGLKRHYLKLSFSTVDELVKVRKEISPAVRKNRERDQANDVYTSMLSSALAGGSLTTSEEGASKKVTNQMDNIVDMREYDVPYHMRLSIDLKIHVAHWYNVRYRGSTFPPEITRRDDLVERPDPVVLAFDIETTKLPLKFPDPETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIIVTYNGDFFDWPFVEARAAAHGINMYQEIGFQKDSQGEYKASQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEEMCRMATEEPQTLATYSVSDAVATYYMYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAYHANIIFPNKQEQEFNKLTEDGHVLDSETYVGGHVEALESGVFRSDIPCRFKMNPAAFDYLVQNVEKTLRHAIEEEERLPLDQVTNFQEVCDEIKVKLNSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRRMTWQWRGEFMSASRSEYHRIQQQLESEKFPPLYPDGAPRAFHELSREEQAKYEKKRLADYCRKAYKKIHVTKVEERVTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAMETGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKSTNAKKPKVTISYPGAMLNILVKEGFTNDQYQELQDPASLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLVKIFQSSVFEAFLKGTTLEEVYASVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRFIISKKPEGSPVTERAIPLAIFQAEPSVRKHYLRKWLKSPSLQDFNIRAILDWDYYIERLGSTIQKIITIPAALQQVKNPVPRVRHPDWLHKKLLEKNDVYKQKKINELFTSEGKRQVLANQPQEGTPSSQVGDIEDFGAAKSLHPSVPIANKRKRVPTAEESQQMSQSLELSQSWREILGPPPSMGTTKEELVAWLRFHQKKWELQARQRRERQKRRRLEDGGTAAGGGVVRDALSRGLGSYLRHTARSILDLPWQIVQIAETSQPGLFRLWAVIGSDLHCIKLSIPRVFYVNQRVAKPEEGAVYRKVNRILPRSNLVYNLYEYSVPEDMYQEHINEINADLSAPDIEGVYETQVPLLLRALIQLGCVCMVSRQLVRHLTGREADTFDIEHLEMRSLAQFPYLEPGSIRHIYLYHSSQGSRALFGLFIPAQRKAAVFVLDTVRSNQMPNLTTMFSAERSALLERVGEELLPPDKHTFEVRAETDPKAICRAIQRLLLGYKDERRGPTLIAVQSNWELKRLASGIPILDEFPLVPIRVTDDISYSVLDWQRHAARRMIRHYLNLDTCLSQAFEMSRYYHIPIGNLPDDISTFGSDLFFSRHLRRHNHLLWLSPTARPDLGGKEADDNRLVMEFDERASVEINNPGCYSTVCLELDIQSLAVNTVLQSHHVNDMEGCSSMSISFDVIQQASLEDMVTGNQAANIPASYDETALCSNTFRILKSMVVSWVKEITQYHNIYADNQVIHFYRWLRSPSSLLYDPALHRTLHNMMKKLFLQLVAEFRRLGSSVVYANFNRIILCTKKRRIEDAISYMEYIINSIHSKEIFHSLTISFSRCWEFLLWMDPANYGGIKGKVHSRIHCGEDTSKKQAPEEGDSEEEEEAGEDDEEEGESNVEELLENSWNIVQFLPQAASCQNYFLMIVSAYIVAVYHSMKEELRRNAPGSTPVKRRGTSQVTQEAQGETGAMPGMITFSQDYVANELTQSFFTITQKIQKKMSGSRHTTEPSDLFPVLPGSYLPLNNPALEFIKYVCQVLSLDANVTNQVNKLRRDLLRLIEVGEFSDEAQFRDPCRSYVLPEVICRNCNFCRDLDLCKDPALSQDGSLLPSWVCSNCQAQYDSDSIEMALVEALQKKLMAFMLQDLVCKKCHGVKDTHMPLYCSCAGDFALTISSQTFMEHITVFQNIARHYGMAHLLETIEWLLHTNPQLQQ

Target ORF information:

RefSeq Version	XM_001233984.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus DNA polymerase epsilon, catalytic subunit (POLE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001233984.5

ORF Insert Sequence:

ATGGTGCTGC GGGGCGGCGG CGGCGGCGAC CGTGACCCCG AGGGCACCCG GGATGATGGC 
CCCGCGCTGT CTGCCCTCAA GCGCCTGGAG CGGTCGCAGC GCACGGACCA GTTGGACGCG 
CTGTTCGGCT TCGAGCGACC CGCCGAGCCC TGCGAGCGGA CGGGCTGGCT CATCAACATG 
CACCCGACCG AGGTGCTGGA TGAGGACCGG CGGGCAGTGA GCGCTGTGGA CTATTACTTC 
ATCCAGGAGG ATGGCAGCCG CTTCAAGGTG GCCCTGCCCT ACAGACCCTA CTTCTATGTG 
GCCACCCAGC AGGGCTGCGA GCGGGAGGTG TCCTCCTTCC TGGCCAAGAA GTTCCAAGGG 
AAGATTGCGA AGCTGGAAAC TGTCCCAAAG GAAGATTTGG ATCTGCCCAA CCACCTGGTG 
GGGCTGAAGC GGCACTACCT CAAGCTGTCC TTCAGCACAG TGGATGAGTT GGTGAAGGTG 
AGGAAAGAGA TCTCCCCTGC TGTAAGGAAG AACAGGGAGC GCGACCAGGC CAATGATGTG 
TACACATCCA TGCTGTCAAG TGCTCTGGCT GGTGGCAGCC TGACCACATC GGAGGAGGGA 
GCCTCCAAAA AGGTCACCAA TCAAATGGAC AACATTGTGG ACATGCGGGA GTACGACGTG 
CCCTACCACA TGCGTCTCTC CATCGACCTC AAGATCCACG TGGCTCACTG GTACAACGTG 
CGGTACCGCG GCAGCACCTT CCCCCCCGAG ATCACCCGCA GGGATGACCT GGTGGAGCGG 
CCGGATCCTG TTGTCCTCGC CTTTGACATT GAAACCACAA AACTCCCCTT GAAGTTTCCT 
GATCCAGAGA CGGACCAGAT CATGATGATC TCCTACATGA TTGATGGGCA GGGCTACCTG 
ATCACAAATA GGGAGATCGT CTCTGAGGAT ATCGAAGACT TTGAGTTCAC TCCCAAGCCG 
GAGTACGAGG GTCCCTTTTG TGTCTTTAAC GAGCCAGATG AAGCTCATTT AATCCAGAGA 
TGGTTTGAAC ACGTCCAGGA GACCAAACCT ACCATCATTG TCACATACAA TGGAGACTTC 
TTTGACTGGC CCTTTGTAGA AGCAAGAGCC GCAGCTCATG GAATTAACAT GTACCAGGAA 
ATTGGATTTC AGAAGGACAG CCAGGGAGAG TACAAAGCAT CACAGTGCAT CCACATGGAC 
TGCCTAAGGT GGGTGAAGAG AGACAGCTAC CTCCCAGTGG GAAGTCACAA CCTGAAGGCA 
GCAGCTAAAG CAAAACTGGG CTATGACCCA GTGGAGCTGG ACCCTGAAGA GATGTGCAGG 
ATGGCTACAG AGGAGCCTCA GACCCTGGCC ACCTATTCAG TGTCCGATGC TGTTGCCACC 
TATTACATGT ATATGAAGTA TGTGCACCCT TTCATTTTTG CCTTGTGCAC CATCATTCCC 
ATGGAGCCCG ATGAGGTGTT ACGAAAAGGT TCTGGGACGC TGTGCGAGGC GCTGCTGATG 
GTTCAAGCCT ATCATGCCAA CATCATCTTC CCCAACAAGC AGGAGCAGGA ATTCAACAAG 
CTCACAGAGG ATGGCCACGT GCTGGACTCG GAGACGTACG TTGGAGGCCA CGTGGAAGCA 
CTGGAGTCGG GGGTGTTCCG CAGTGACATC CCCTGCCGCT TCAAAATGAA CCCTGCTGCC 
TTTGACTACT TGGTGCAGAA CGTGGAGAAG ACGCTCCGGC ACGCTATTGA GGAGGAGGAG 
CGTTTACCGT TGGATCAAGT CACCAACTTC CAGGAGGTCT GTGATGAAAT CAAAGTCAAA 
CTGAATTCCC TGAAGGATGT TCCTAACCGA ATTGAGTGTC CCCTTATCTA CCATTTAGAT 
GTGGGAGCCA TGTACCCCAA CATCATTCTG ACCAACAGAT TGCAGCCCTC AGCAATGGTG 
GATGAGGCCA CATGTGCTGC CTGTGACTTC AACAAGCCAG GGGCCAACTG CCAGCGCCGG 
ATGACGTGGC AATGGAGAGG AGAATTCATG TCTGCGAGCC GCAGTGAGTA CCACCGCATC 
CAGCAGCAAT TGGAGTCGGA GAAGTTCCCT CCACTGTACC CTGATGGAGC ACCCCGTGCT 
TTCCACGAGC TCTCCCGAGA AGAACAAGCC AAGTATGAGA AGAAACGGCT GGCAGATTAC 
TGTCGCAAAG CCTACAAGAA GATCCATGTC ACCAAGGTGG AGGAGAGGGT CACCACCATC 
TGCCAACGAG AGAACTCTTT CTATGTGGAC ACGGTGCGAG CTTTCAGGGA CCGCCGCTAT 
GAGTTCAAGG GGCTGCACAA GGTATGGAAG AAGAAGCTGT CTGCAGCCAT GGAGACAGGA 
GATGCCTCTG AAGTGAAACG CTGCAAGAAC ATGGAGATCC TCTATGACTC CCTGCAGTTG 
GCACACAAGT GCATTCTCAA TTCTTTCTAT GGCTATGTCA TGCGGAAGGG AGCTCGATGG 
TACTCCATGG AAATGGCCGG GATTGTCTGC TTCACCGGAG CCAATATCAT CACACAGGCC 
AGGGAACTGA TTGAGCAGAT TGGGAGACCT CTGGAACTGG ATACAGATGG GATTTGGTGT 
GTCCTCCCCA ACAGCTTCCC AGAAAACTTT GTCATCAAGT CAACCAATGC CAAGAAGCCA 
AAGGTGACAA TCTCCTACCC AGGTGCTATG CTGAACATCC TGGTGAAGGA AGGCTTCACA 
AACGATCAGT ACCAGGAGCT GCAGGACCCA GCCTCTCTCA CCTACGTCAC ACGCTCGGAG 
AACAGCATCT TCTTTGAAGT GGATGGGCCA TACCTGGCCA TGATTCTCCC AGCTTCCAAG 
GAGGAGGGAA AGAAGCTGAA GAAAAGGTAT GCGGTGTTCA ATGAGGATGG GTCCCTGGCT 
GAGCTGAAGG GCTTTGAGGT GAAGCGCCGG GGCGAGCTGC AGCTGGTGAA GATATTCCAG 
TCTTCTGTAT TCGAGGCCTT CCTGAAAGGC ACCACCCTGG AGGAGGTCTA TGCTTCTGTG 
GCCAAGGTGG CTGACTACTG GCTGGATGTG CTGTACAGCA AGGCTGCCAA CATGCCCGAT 
TCGGAGCTGT TTGAGCTCAT CTCAGAGAAC CGTTCCATGT CTCGCAAGCT GGAGGACTAT 
GGGGAGCAGA AATCCACCTC CATCAGCACT GCCAAGCGCC TGGCAGAGTT CCTGGGGGAC 
CAGATGGTGA AGGATGCAGG GCTGAGCTGC CGCTTCATCA TCTCCAAGAA ACCTGAGGGC 
TCTCCTGTCA CTGAGAGGGC CATCCCCCTT GCCATCTTCC AAGCAGAGCC CAGCGTACGG 
AAGCACTACC TCCGGAAATG GCTGAAGAGC CCCTCGCTGC AGGACTTCAA CATTCGGGCA 
ATTCTGGACT GGGACTACTA CATTGAGAGA CTGGGAAGCA CCATCCAGAA AATCATCACT 
ATTCCTGCAG CCCTGCAGCA GGTAAAGAAC CCAGTGCCAC GGGTCCGACA CCCGGACTGG 
CTGCACAAGA AACTCCTGGA GAAGAATGAT GTGTACAAAC AGAAAAAGAT CAATGAGCTC 
TTCACTTCTG AAGGCAAGAG GCAGGTCCTC GCCAACCAGC CCCAGGAGGG CACACCCAGC 
TCGCAGGTTG GTGACATAGA GGATTTTGGG GCTGCCAAGA GCCTTCATCC ATCCGTTCCC 
ATTGCAAACA AACGGAAGCG AGTCCCTACA GCAGAGGAAA GCCAACAGAT GTCCCAGAGC 
TTGGAGCTGT CCCAGTCCTG GCGGGAGATC CTGGGTCCTC CTCCCTCCAT GGGCACCACC 
AAGGAGGAGC TCGTGGCCTG GCTGCGCTTC CACCAGAAGA AATGGGAGCT GCAGGCCCGG 
CAGCGGCGCG AGCGGCAGAA GAGGCGGCGC CTGGAGGACG GTGGGACGGC GGCGGGCGGC 
GGGGTGGTCC GTGATGCCCT CTCCAGGGGG CTGGGCAGCT ACTTGCGCCA CACTGCGCGC 
AGCATCCTGG ACTTGCCCTG GCAGATCGTG CAGATCGCTG AGACCAGCCA GCCTGGCTTG 
TTCAGGCTAT GGGCGGTGAT CGGGAGCGAC CTGCACTGCA TCAAGCTGAG CATCCCCAGG 
GTGTTCTACG TCAACCAGCG CGTCGCAAAG CCGGAGGAGG GAGCGGTCTA TAGGAAGGTG 
AACAGGATCC TGCCCCGATC AAACCTGGTG TACAACCTGT ACGAATACTC AGTCCCTGAG 
GACATGTACC AGGAGCACAT CAACGAGATC AATGCTGACC TCTCTGCTCC CGACATCGAG 
GGAGTGTATG AGACTCAGGT GCCACTGCTG CTGCGAGCCC TGATCCAGCT GGGCTGTGTC 
TGCATGGTCA GCAGGCAGCT CGTCCGGCAC CTGACGGGCC GTGAGGCGGA CACCTTCGAC 
ATCGAGCACC TGGAGATGCG CTCGCTGGCC CAGTTCCCCT ACCTGGAGCC AGGGAGCATC 
CGCCACATCT ACCTCTACCA TAGCTCCCAG GGCAGCAGGG CCCTCTTCGG CCTCTTCATC 
CCCGCGCAGC GCAAGGCTGC CGTCTTTGTG CTGGACACGG TGCGCAGCAA CCAGATGCCA 
AACCTCACCA CCATGTTCTC AGCAGAGCGC AGCGCGCTGC TGGAAAGGGT GGGTGAGGAG 
CTGCTGCCTC CAGACAAGCA CACCTTCGAA GTGCGCGCCG AGACTGACCC CAAGGCCATC 
TGCAGAGCCA TCCAGCGGCT GCTGCTTGGT TACAAGGACG AGCGCCGGGG TCCCACGCTG 
ATCGCTGTGC AGTCCAACTG GGAGCTGAAG CGGCTGGCGA GCGGTATTCC CATCCTCGAC 
GAGTTCCCCC TGGTCCCCAT CCGGGTGACA GATGACATCA GCTACTCGGT CCTGGACTGG 
CAGCGCCACG CAGCCCGACG CATGATCCGC CATTACCTCA ACCTGGACAC ATGCCTGTCG 
CAGGCCTTTG AGATGAGCAG ATATTACCAC ATTCCCATCG GGAACCTCCC TGATGACATC 
TCCACCTTTG GCTCTGACCT GTTCTTCTCA CGGCACCTCC GTCGTCACAA CCATTTGCTG 
TGGCTCTCAC CCACGGCCCG CCCAGACCTG GGGGGGAAGG AGGCTGATGA CAACCGCCTC 
GTCATGGAGT TCGATGAGAG AGCCTCAGTG GAGATAAACA ACCCCGGCTG CTATTCCACG 
GTGTGTCTGG AGCTGGACAT CCAGAGCCTG GCTGTGAACA CAGTGCTGCA ATCTCATCAT 
GTGAACGATA TGGAAGGATG TTCCAGCATG AGCATCAGCT TCGACGTAAT TCAGCAGGCA 
TCCTTGGAAG ATATGGTGAC AGGGAACCAA GCTGCCAACA TCCCAGCCAG CTATGATGAA 
ACCGCCCTCT GCTCCAACAC ATTCAGGATT CTGAAGAGCA TGGTGGTGAG CTGGGTGAAG 
GAGATCACAC AGTACCACAA CATCTATGCA GACAACCAGG TCATTCACTT CTACCGCTGG 
CTTCGCTCTC CTTCCTCGCT GCTCTACGAC CCGGCGCTGC ACCGCACGTT GCACAACATG 
ATGAAGAAGC TTTTCCTTCA GTTGGTGGCT GAGTTCAGGC GCCTCGGCTC TTCGGTGGTA 
TACGCCAACT TCAACCGGAT CATTCTGTGC ACCAAGAAGC GGCGTATCGA GGACGCCATC 
AGCTACATGG AGTACATTAT CAACAGCATC CACTCAAAGG AGATCTTCCA CTCTCTGACT 
ATTTCCTTCT CCCGCTGCTG GGAGTTCCTG CTCTGGATGG ATCCAGCCAA TTATGGAGGT 
ATCAAGGGAA AAGTGCATTC TCGCATCCAC TGCGGGGAGG ATACCAGCAA GAAGCAAGCA 
CCAGAGGAGG GAGACAGTGA GGAAGAGGAG GAGGCAGGTG AAGATGATGA GGAAGAAGGG 
GAGTCCAATG TGGAGGAGCT GCTGGAGAAC AGCTGGAACA TCGTGCAGTT CCTGCCGCAG 
GCTGCCTCCT GCCAGAACTA CTTCCTGATG ATTGTGTCAG CCTACATCGT GGCCGTGTAC 
CATAGCATGA AGGAGGAGCT GCGGCGCAAT GCCCCCGGCA GCACTCCCGT CAAGAGAAGA 
GGCACCAGCC AGGTGACCCA GGAGGCACAG GGCGAGACAG GAGCCATGCC TGGGATGATC 
ACCTTCTCAC AGGATTACGT GGCCAATGAG CTCACTCAGA GCTTCTTCAC CATCACACAG 
AAGATCCAGA AGAAGATGTC TGGCTCCCGA CACACCACTG AGCCATCAGA CTTGTTCCCG 
GTGCTGCCCG GCTCCTACTT GCCGCTCAAC AACCCTGCTC TGGAGTTCAT CAAATACGTT 
TGCCAGGTGC TGTCCCTGGA TGCCAACGTC ACCAACCAGG TGAACAAGCT GCGCAGGGAC 
CTGCTGCGCC TCATCGAGGT GGGCGAGTTC TCAGATGAAG CCCAGTTTCG GGACCCTTGC 
CGCTCCTATG TGCTCCCTGA AGTCATCTGC AGAAACTGCA ACTTCTGCAG GGACCTGGAC 
CTGTGCAAGG ACCCAGCCCT CTCCCAGGAC GGATCGCTGC TGCCCAGCTG GGTGTGCTCC 
AACTGCCAGG CACAGTACGA CTCCGACTCC ATAGAAATGG CCTTGGTGGA AGCCCTGCAG 
AAGAAGCTGA TGGCCTTCAT GCTGCAGGAC CTGGTGTGCA AGAAGTGCCA CGGTGTGAAG 
GACACACACA TGCCTTTGTA CTGCAGCTGT GCTGGAGACT TTGCCCTCAC CATCTCCTCC 
CAGACCTTCA TGGAGCACAT CACCGTCTTC CAGAACATCG CCAGGCACTA CGGCATGGCC 
CATCTGCTGG AAACCATCGA ATGGCTGCTG CACACCAACC CCCAGCTCCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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POLE cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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