Rock2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rock2
Entrez Gene ID	25537
Full Name	Rho-associated coiled-coil containing protein kinase 2
Synonyms	ROCK-II,ROK
General protein information	Preferred Names Rho-associated coiled-coil containing protein kinase 2 Names rho-associated protein kinase 2 ROKalpha Rho-associated coiled-coil forming kinase 2 RhoA - binding serine/threosine kinase alpha (ROK - alpha) p150 ROK-alpha p164 ROCK-2 rho-associated, coiled-coil-containing protein kinase 2 rho-associated, coiled-coil-containing protein kinase II rhoA-binding kinase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q16
Summary	a Rho kinase that is involved in inducing membrane blebbing and chromatin condensation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006239909.3	XP_006239971.1	rho-associated protein kinase 2 isoform X3
XM_008764574.1	XP_008762796.1	rho-associated protein kinase 2 isoform X1
XM_008764575.2	XP_008762797.1	rho-associated protein kinase 2 isoform X2
NM_013022.2	NP_037154.2	rho-associated protein kinase 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rock2	NP_033098.2
Drosophila melanogaster (fruit fly)	rok	NP_536796.2
Caenorhabditis elegans (roundworm)	let-502	NP_491440.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000406	XP_310694.5
Pan troglodytes (chimpanzee)	ROCK2	XP_525689.3
Rattus norvegicus (Norway rat)	Rock2	NP_037154.2
Xenopus tropicalis (tropical clawed frog)	rock2	XP_004914525.1
Homo sapiens (human)	ROCK2	NP_004841.2
Macaca mulatta (Rhesus monkey)	ROCK2	XP_001096931.2
Canis lupus familiaris (dog)	ROCK2	XP_540083.4
Danio rerio (zebrafish)	rock2a	NP_777288.1
Bos taurus (cattle)	ROCK2	NP_776877.1
Gallus gallus (chicken)	ROCK2	XP_004935820.1

Gene Ontology
Bibliography

Related articles in PubMed

miR‑335 promotes stress granule formation to inhibit apoptosis by targeting ROCK2 in acute ischemic stroke.
Si W, Ye S, Ren Z, Liu X, Wu Z, Li Y, Zhou J, Zhang S, Li Y, Deng R, Chen D
International journal of molecular medicine43(3)1452-1466(2019 Mar)

ROCK2 regulates autophagy in the hippocampus of rats after subarachnoid hemorrhage.
Sun L, Ma Y, Zhang Z, Li X, Chen Y, Liu G, Fu A
Neuroreport29(18)1571-1577(2018 12)

RhoA/ROCK signaling regulates smooth muscle phenotypic modulation and vascular remodeling via the JNK pathway and vimentin cytoskeleton.
Tang L, Dai F, Liu Y, Yu X, Huang C, Wang Y, Yao W
Pharmacological research133201-212(2018 07)

Exercise increases Rho-kinase activity and insulin signaling in skeletal muscle.
Muñoz VR, Gaspar RC, Kuga GK, da Rocha AL, Crisol BM, Botezelli JD, Baptista IL, Mekary RA, da Silva ASR, Cintra DE, de Moura LP, Ropelle ER, Pauli JR
Journal of cellular physiology233(6)4791-4800(2018 06)

Neuroprotection Mediated by Upregulation of Endothelial Nitric Oxide Synthase in Rho-Associated, Coiled-Coil-Containing Kinase 2 Deficient Mice.
Hiroi Y, Noma K, Kim HH, Sladojevic N, Tabit CE, Li Y, Soydan G, Salomone S, Moskowitz MA, Liao JK
Circulation journal : official journal of the Japanese Circulation Society82(4)1195-1204(2018 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rock2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rock2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01674	NM_013022.2 Latest version!	Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa29219	XM_006239909.3 Latest version!	Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa43135	XM_008764574.1 Latest version!	Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa43136	XM_008764575.2 Latest version!	Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rock2 ( NM_013022.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037154.2 Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), mRNA.

Price & Availability↑

CloneID	ORa01674
Clone ID Related Accession (Same CDS sequence)	NM_013022.2
Accession Version	NM_013022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Rattus norvegicus(Norway rat)
Product	rho-associated protein kinase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07063692.1, U38481.1, CB691694.1, AABR07063695.1, CK475383.1 and BF398321.1. On Jul 31, 2012 this sequence version replaced NM_013022.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U38481.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGGCCGC GCCGGGGGAC 
GGGGCGGGCG CGGGTCGTCA GAGGAAGCTG GAGGCGCTCA TCCGAGACCC TCGCTCGCCC 
ATCAACGTGG AAAGCCTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAATAAAAA TATAGATAAT TTCCTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTTTACAGAT GAAAGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTTCA GTTGGTTCGT CATAAGGCAT CACAGAAGGT TTATGCAATG 
AAGCTTCTTA GTAAATTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAAGAA 
AGAGATATTA TGGCTTTTGC TAACAGTCCG TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGACAGGT ATCTGTACAT GGTGATGGAG TACATGCCAG GTGGAGACCT TGTTAACCTG 
ATGAGCAATT ATGATGTGCC TGAGAAGTGG GCCAAGTTTT ATACTGCTGA AGTCGTGCTT 
GCACTGGATG CAATACACTC CATGGGCTTA ATACACAGAG ATGTGAAGCC CGATAACATG 
CTATTGGATA AACACGGACA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAAATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACTC CAGACTACAT ATCACCTGAG 
GTTCTGAAAT CACAAGGAGG TGATGGTTAC TATGGACGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTTTGAGAT GTTAGTAGGG GACACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACCTACA GCAAAATTAT GGATCATAAA AATTCACTGT GTTTTCCTGA AGATACAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATATGT GCCTTCTTAA CAGACAGGGA GGTACGACTT 
GGAAGAAATG GGGTAGAAGA AATCAAACAG CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC GGCAGCTCCA GTGGTGCCTG AGCTCAGCAG TGACATAGAC 
AGCAGCAACT TTGATGACAT TGAGGATGAC AAAGGCGATG TAGAGACCTT CCCGATTCCT 
AAAGCTTTTG TGGGAAATCA GCTGCCTTTT ATAGGATTTA CCTACTTTAG AGAAAATTTG 
TTACTAAGTG ATTCTCCACC TTGTAGAGAA AATGATGCAA TACAAACAAG GAAAAGTGAA 
GAAAGTCAGG AGATTCAGAA AAAATTATAT GCACTAGAAG AACACCTTAG CAGTGAGGTA 
CAAGCCAAGG AGGAACTGGA ACAGAAGTGC AAGTCTATTA ATACTCGTCT AGAGAAAACA 
GCAAAGGAAC TAGAAGAAGA GATTACCTTT AGGAAAAATG TGGAATCAAC ATTAAGACAG 
CTAGAAAGAG AAAAGGCCCT TCTTCAGCAC AAAAATGCAG AATATCAACG GAAAGCTGAT 
CATGAAGCTG ACAAGAAACG GAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATCTGA AGAAAAGAAA CCAGAGCTCT CAGATATCCA CTGAGAAGGT CAATCAGCTC 
CAGAAACAAC TGGATGAAGC TAATGCTTTG CTAAGAACAG AGTCTGATAC TGCAGCACGG 
TTAAGAAAAA CCCAGGCAGA AAGTTCCAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTTGAA ACTGCCAAGT TAAAACTTGA GAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGAAGGGACC GGACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATCTGGG TTGGAAGAAG ATTTGAAGAC TGGCAAAACC 
TTACTAGCAA AAGTAGAGCT GGAGAAGAGA CAGCTGCAGG AGAAGCTCAC TGACTTAGAG 
AAGGAAAAGA GCAACATGGA AATAGATATG ACATATCAAC TGAAAGTTAT ACAGCAGAGT 
TTAGAACAAG AAGAAGCTGA ACACAAGACC ACAAAAGCAC GACTAGCGGA TAAAAATAAG 
ATCTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTG 
TTGGAAGAGA GGTCTTTAAA GCAGAAAGTG GAGAACCTAC TCCTGGAAGC CGAGAAGAGA 
TGTTCTATAT TGGATTGTGA CCTCAAACAG TCTCAGCAGA AACTGAATGA GCTGCTGAAG 
CAGAAAGATG TGCTGAATGA GGATGTTAGA AATTTGACAT TAAAAATAGA GCAGGAAACT 
CAGAAGCGTT GTCTTATGCA AAATGACCTG AAGATGCAGA CCCAGCAAGT TAACACCCTA 
AAAATGTCAG AAAAGCAAAT AAAACAAGAA AATAATCATC TTATGGAAAT GAAAATGAAC 
TTGGAAAAGC AAAATGCTGA ACTTCGAAAA GAGCGGCAGG ATGCAGATGG GCAGATGAAG 
GAACTGCAAG ACCAACTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAAGTT 
AGAGAACTTA AAGAAGAAAA TGAAGAGAAA ACTAAACTTT GTAAAGAGTT GCAGCAGAAA 
AAGCAGGATT TACAGGATGA AAGAGACTCC TTGGCTGCCC AGCTGGAGAT CACCCTCACC 
AAAGCAGACT CTGAGCAGCT GGCTCGCTCC ATTGCTGAGG AGCAATACTC TGACTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAAATC AAAGAGATGA TGGCTAGACA CAAACAAGAG 
CTTACTGAAA AGGACGCTAC AATTGCATCC CTTGAAGAAA CAAATAGAAC ACTAACTAGT 
GATGTTGCAA ATCTTGCAAA CGAGAAAGAA GAACTAAACA ACAAGTTGAA AGATACCCAA 
GAACAACTGT CAAAGTTGAA AGATGAAGAG ATCAGTGCAG CGGCTATTAA AGCACAGTTT 
GAGAAGCAGC TGCTGACTGA GCGAACACTC AAGACTCAAG CTGTGAATAA GTTGGCAGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAGTGACA CAGATGTGCG AAGAAAAGAA 
AAGGAAAACA GAAAATTACA TATGGAGCTT AAGTCTGAAC GTGAAAAATT GACACAACAG 
ATGATCAAAT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGCCAA 
ATTCGAATAG AACTGCAGAT GACCCTGGAC AGTAAAGACA GTGACATTGA ACAGCTTCGG 
TCGCAGCTCC AGGCCTTGCA TATTGGTATG GACAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGC CTGATGACGG ATTTCCAGAA TCACGATTAG AAGGATGGCT GTCGTTGCCT 
GTGCGGAACA ACACTAAAAA GTTTGGATGG GTTAAGAAGT ATGTGATTGT AAGCAGCAAG 
AAGATTCTTT TCTATGATAG TGAACAAGAT AAAGAGCAGT CTAACCCTTA CATGGTGTTA 
GACATAGACA AGCTATTTCA TGTCCGACCT GTTACTCAGA CAGATGTATA CAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTTCAGATC CTGTATGCCA ATGAAGGAGA AAGTAAAAAG 
GAACCAGAAT TTCCAGTGGA ACCAGTGGGA GAAAAATCTA ATTATATTTG CCACAAAGGA 
CATGAGTTTA TTCCTACTCT TTATCACTTC CCAACCAACT GTGAGGCCTG TATGAAGCCA 
CTGTGGCACA TGTTTAAACC TCCTCCTGCT CTAGAGTGCC GTAGATGCCA CATTAAATGT 
CATAAAGATC ACATGGACAA AAAGGAAGAG ATCATAGCCC CCTGCAAAGT TTATTATGAT 
ATATCATCGG CAAAGAATCT GTTGTTATTA GCAAATTCAA CGGAAGAACA GCAGAAATGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCTCCAG CTCCAGACCC TTTTGCCCGA 
TCATCCCCTA GAACCTCAAT GAAAATACAA CAAAACCAGT CCATTCGGCG GCCCAGTCGA 
CAGCTTGCTC CAAACAAACC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037154.2
CDS	49..4215
Translation	MSRPPPTGKMPGAPEAAPGDGAGAGRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITFRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQLQKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRISGLEEDLKTGKTLLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQSQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDTQEQLSKLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGMDSSSIGSGPGDAEPDDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	NM_013022.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013022.2

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGGCCGC GCCGGGGGAC 
GGGGCGGGCG CGGGTCGTCA GAGGAAGCTG GAGGCGCTCA TCCGAGACCC TCGCTCGCCC 
ATCAACGTGG AAAGCCTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAATAAAAA TATAGATAAT TTCCTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTTTACAGAT GAAAGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTTCA GTTGGTTCGT CATAAGGCAT CACAGAAGGT TTATGCAATG 
AAGCTTCTTA GTAAATTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAAGAA 
AGAGATATTA TGGCTTTTGC TAACAGTCCG TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGACAGGT ATCTGTACAT GGTGATGGAG TACATGCCAG GTGGAGACCT TGTTAACCTG 
ATGAGCAATT ATGATGTGCC TGAGAAGTGG GCCAAGTTTT ATACTGCTGA AGTCGTGCTT 
GCACTGGATG CAATACACTC CATGGGCTTA ATACACAGAG ATGTGAAGCC CGATAACATG 
CTATTGGATA AACACGGACA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAAATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACTC CAGACTACAT ATCACCTGAG 
GTTCTGAAAT CACAAGGAGG TGATGGTTAC TATGGACGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTTTGAGAT GTTAGTAGGG GACACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACCTACA GCAAAATTAT GGATCATAAA AATTCACTGT GTTTTCCTGA AGATACAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATATGT GCCTTCTTAA CAGACAGGGA GGTACGACTT 
GGAAGAAATG GGGTAGAAGA AATCAAACAG CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC GGCAGCTCCA GTGGTGCCTG AGCTCAGCAG TGACATAGAC 
AGCAGCAACT TTGATGACAT TGAGGATGAC AAAGGCGATG TAGAGACCTT CCCGATTCCT 
AAAGCTTTTG TGGGAAATCA GCTGCCTTTT ATAGGATTTA CCTACTTTAG AGAAAATTTG 
TTACTAAGTG ATTCTCCACC TTGTAGAGAA AATGATGCAA TACAAACAAG GAAAAGTGAA 
GAAAGTCAGG AGATTCAGAA AAAATTATAT GCACTAGAAG AACACCTTAG CAGTGAGGTA 
CAAGCCAAGG AGGAACTGGA ACAGAAGTGC AAGTCTATTA ATACTCGTCT AGAGAAAACA 
GCAAAGGAAC TAGAAGAAGA GATTACCTTT AGGAAAAATG TGGAATCAAC ATTAAGACAG 
CTAGAAAGAG AAAAGGCCCT TCTTCAGCAC AAAAATGCAG AATATCAACG GAAAGCTGAT 
CATGAAGCTG ACAAGAAACG GAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATCTGA AGAAAAGAAA CCAGAGCTCT CAGATATCCA CTGAGAAGGT CAATCAGCTC 
CAGAAACAAC TGGATGAAGC TAATGCTTTG CTAAGAACAG AGTCTGATAC TGCAGCACGG 
TTAAGAAAAA CCCAGGCAGA AAGTTCCAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTTGAA ACTGCCAAGT TAAAACTTGA GAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGAAGGGACC GGACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATCTGGG TTGGAAGAAG ATTTGAAGAC TGGCAAAACC 
TTACTAGCAA AAGTAGAGCT GGAGAAGAGA CAGCTGCAGG AGAAGCTCAC TGACTTAGAG 
AAGGAAAAGA GCAACATGGA AATAGATATG ACATATCAAC TGAAAGTTAT ACAGCAGAGT 
TTAGAACAAG AAGAAGCTGA ACACAAGACC ACAAAAGCAC GACTAGCGGA TAAAAATAAG 
ATCTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTG 
TTGGAAGAGA GGTCTTTAAA GCAGAAAGTG GAGAACCTAC TCCTGGAAGC CGAGAAGAGA 
TGTTCTATAT TGGATTGTGA CCTCAAACAG TCTCAGCAGA AACTGAATGA GCTGCTGAAG 
CAGAAAGATG TGCTGAATGA GGATGTTAGA AATTTGACAT TAAAAATAGA GCAGGAAACT 
CAGAAGCGTT GTCTTATGCA AAATGACCTG AAGATGCAGA CCCAGCAAGT TAACACCCTA 
AAAATGTCAG AAAAGCAAAT AAAACAAGAA AATAATCATC TTATGGAAAT GAAAATGAAC 
TTGGAAAAGC AAAATGCTGA ACTTCGAAAA GAGCGGCAGG ATGCAGATGG GCAGATGAAG 
GAACTGCAAG ACCAACTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAAGTT 
AGAGAACTTA AAGAAGAAAA TGAAGAGAAA ACTAAACTTT GTAAAGAGTT GCAGCAGAAA 
AAGCAGGATT TACAGGATGA AAGAGACTCC TTGGCTGCCC AGCTGGAGAT CACCCTCACC 
AAAGCAGACT CTGAGCAGCT GGCTCGCTCC ATTGCTGAGG AGCAATACTC TGACTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAAATC AAAGAGATGA TGGCTAGACA CAAACAAGAG 
CTTACTGAAA AGGACGCTAC AATTGCATCC CTTGAAGAAA CAAATAGAAC ACTAACTAGT 
GATGTTGCAA ATCTTGCAAA CGAGAAAGAA GAACTAAACA ACAAGTTGAA AGATACCCAA 
GAACAACTGT CAAAGTTGAA AGATGAAGAG ATCAGTGCAG CGGCTATTAA AGCACAGTTT 
GAGAAGCAGC TGCTGACTGA GCGAACACTC AAGACTCAAG CTGTGAATAA GTTGGCAGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAGTGACA CAGATGTGCG AAGAAAAGAA 
AAGGAAAACA GAAAATTACA TATGGAGCTT AAGTCTGAAC GTGAAAAATT GACACAACAG 
ATGATCAAAT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGCCAA 
ATTCGAATAG AACTGCAGAT GACCCTGGAC AGTAAAGACA GTGACATTGA ACAGCTTCGG 
TCGCAGCTCC AGGCCTTGCA TATTGGTATG GACAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGC CTGATGACGG ATTTCCAGAA TCACGATTAG AAGGATGGCT GTCGTTGCCT 
GTGCGGAACA ACACTAAAAA GTTTGGATGG GTTAAGAAGT ATGTGATTGT AAGCAGCAAG 
AAGATTCTTT TCTATGATAG TGAACAAGAT AAAGAGCAGT CTAACCCTTA CATGGTGTTA 
GACATAGACA AGCTATTTCA TGTCCGACCT GTTACTCAGA CAGATGTATA CAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTTCAGATC CTGTATGCCA ATGAAGGAGA AAGTAAAAAG 
GAACCAGAAT TTCCAGTGGA ACCAGTGGGA GAAAAATCTA ATTATATTTG CCACAAAGGA 
CATGAGTTTA TTCCTACTCT TTATCACTTC CCAACCAACT GTGAGGCCTG TATGAAGCCA 
CTGTGGCACA TGTTTAAACC TCCTCCTGCT CTAGAGTGCC GTAGATGCCA CATTAAATGT 
CATAAAGATC ACATGGACAA AAAGGAAGAG ATCATAGCCC CCTGCAAAGT TTATTATGAT 
ATATCATCGG CAAAGAATCT GTTGTTATTA GCAAATTCAA CGGAAGAACA GCAGAAATGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCTCCAG CTCCAGACCC TTTTGCCCGA 
TCATCCCCTA GAACCTCAAT GAAAATACAA CAAAACCAGT CCATTCGGCG GCCCAGTCGA 
CAGCTTGCTC CAAACAAACC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rock2 ( XM_006239909.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239971.1 Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa29219
Clone ID Related Accession (Same CDS sequence)	XM_006239909.3
Accession Version	XM_006239909.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	rho-associated protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGGCCGC GCCGGGGGAC 
GGGGCGGGCG CGGGTCGTCA GAGGAAGCTG GAGGCGCTCA TCCGAGACCC TCGCTCGCCC 
ATCAACGTGG AAAGCCTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAATAAAAA TATAGATAAT TTCCTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTTTACAGAT GAAAGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTTCA GTTGGTTCGT CATAAGGCAT CACAGAAGGT TTATGCAATG 
AAGCTTCTTA GTAAATTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAAGAA 
AGAGATATTA TGGCTTTTGC TAACAGTCCG TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGACAGGT ATCTGTACAT GGTGATGGAG TACATGCCAG GTGGAGACCT TGTTAACCTG 
ATGAGCAATT ATGATGTGCC TGAGAAGTGG GCCAAGTTTT ATACTGCTGA AGTCGTGCTT 
GCACTGGATG CAATACACTC CATGGGCTTA ATACACAGAG ATGTGAAGCC CGATAACATG 
CTATTGGATA AACACGGACA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAAATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACTC CAGACTACAT ATCACCTGAG 
GTTCTGAAAT CACAAGGAGG TGATGGTTAC TATGGACGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTTTGAGAT GTTAGTAGGG GACACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACCTACA GCAAAATTAT GGATCATAAA AATTCACTGT GTTTTCCTGA AGATACAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATATGT GCCTTCTTAA CAGACAGGGA GGTACGACTT 
GGAAGAAATG GGGTAGAAGA AATCAAACAG CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC GGCAGCTCCA GTGGTGCCTG AGCTCAGCAG TGACATAGAC 
AGCAGCAACT TTGATGACAT TGAGGATGAC AAAGGCGATG TAGAGACCTT CCCGATTCCT 
AAAGCTTTTG TGGGAAATCA GCTGCCTTTT ATAGGATTTA CCTACTTTAG AGAAAATTTG 
TTACTAAGTG ATTCTCCACC TTGTAGAGAA AATGATGCAA TACAAACAAG GAAAAGTGAA 
GAAAGTCAGG AGATTCAGAA AAAATTATAT GCACTAGAAG AACACCTTAG CAGTGAGGTA 
CAAGCCAAGG AGGAACTGGA ACAGAAGTGC AAGTCTATTA ATACTCGTCT AGAGAAAACA 
GCAAAGGAAC TAGAAGAAGA GATTACCTTT AGGAAAAATG TGGAATCAAC ATTAAGACAG 
CTAGAAAGAG AAAAGGCCCT TCTTCAGCAC AAAAATGCAG AATATCAACG GAAAGCTGAT 
CATGAAGCTG ACAAGAAACG GAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATCTGA AGAAAAGAAA CCAGAGCTCT CAGATATCCA CTGAGAAGGT CAATCAGCTC 
CAGAAACAAC TGGATGAAGC TAATGCTTTG CTAAGAACAG AGTCTGATAC TGCAGCACGG 
TTAAGAAAAA CCCAGGCAGA AAGTTCCAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTTGAA ACTGCCAAGT TAAAACTTGA GAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGAAGGGACC GGACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATCTGGG TTGGAAGAAG ATTTGAAGAC TGGCAAAACC 
TTACTAGCAA AAGTAGAGCT GGAGAAGAGA CAGCTGCAGG AGAAGCTCAC TGACTTAGAG 
AAGGAAAAGA GCAACATGGA AATAGATATG ACATATCAAC TGAAAGTTAT ACAGCAGAGT 
TTAGAACAAG AAGAAGCTGA ACACAAGACC ACAAAAGCAC GACTAGCGGA TAAAAATAAG 
ATCTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTG 
TTGGAAGAGA GGTCTTTAAA GCAGAAAGTG GAGAACCTAC TCCTGGAAGC CGAGAAGAGA 
TGTTCTATAT TGGATTGTGA CCTCAAACAG TCTCAGCAGA AACTGAATGA GCTGCTGAAG 
CAGAAAGATG TGCTGAATGA GGATGTTAGA AATTTGACAT TAAAAATAGA GCAGGAAACT 
CAGAAGCGTT GTCTTATGCA AAATGACCTG AAGATGCAGA CCCAGCAAGT TAACACCCTA 
AAAATGTCAG AAAAGCAAAT AAAACAAGAA AATAATCATC TTATGGAAAT GAAAATGAAC 
TTGGAAAAGC AAAATGCTGA ACTTCGAAAA GAGCGGCAGG ATGCAGATGG GCAGATGAAG 
GAACTGCAAG ACCAACTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAAGTT 
AGAGAACTTA AAGAAGAAAA TGAAGAGAAA ACTAAACTTT GTAAAGAGTT GCAGCAGAAA 
AAGCAGGATT TACAGGATGA AAGAGACTCC TTGGCTGCCC AGCTGGAGAT CACCCTCACC 
AAAGCAGACT CTGAGCAGCT GGCTCGCTCC ATTGCTGAGG AGCAATACTC TGACTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAAATC AAAGAGATGA TGGCTAGACA CAAACAAGAG 
CTTACTGAAA AGGACGCTAC AATTGCATCC CTTGAAGAAA CAAATAGAAC ACTAACTAGT 
GATGTTGCAA ATCTTGCAAA CGAGAAAGAA GAACTAAACA ACAAGTTGAA AGATACCCAA 
GAACAACTGT CAAAGTTGAA AGATGAAGAG ATCAGTGCAG CGGCTATTAA AGCACAGTTT 
GAGAAGCAGC TGCTGACTGA GCGAACACTC AAGACTCAAG CTGTGAATAA GTTGGCAGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAGTGACA CAGATGTGCG AAGAAAAGAA 
AAGGAAAACA GAAAATTACA TATGGAGCTT AAGTCTGAAC GTGAAAAATT GACACAACAG 
ATGATCAAAT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGCCAA 
ATTCGAATAG AACTGCAGAT GACCCTGGAC AGTAAAGACA GTGACATTGA ACAGCTTCGG 
TCGCAGCTCC AGGCCTTGCA TATTGGTATG GACAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGC CTGATGACGG ATTTCCAGAA TCACGATTAG AAGGATGGCT GTCGTTGCCT 
GTGCGGAACA ACACTAAAAA GTTTGGATGG GTTAAGAAGT ATGTGATTGT AAGCAGCAAG 
AAGATTCTTT TCTATGATAG TGAACAAGAT AAAGAGCAGT CTAACCCTTA CATGGTGTTA 
GACATAGACA AGCTATTTCA TGTCCGACCT GTTACTCAGA CAGATGTATA CAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTTCAGATC CTGTATGCCA ATGAAGGAGA AAGTAAAAAG 
GAACCAGAAT TTCCAGTGGA ACCAGTGGGA GAAAAATCTA ATTATATTTG CCACAAAGGA 
CATGAGTTTA TTCCTACTCT TTATCACTTC CCAACCAACT GTGAGGCCTG TATGAAGCCA 
CTGTGGCACA TGTTTAAACC TCCTCCTGCT CTAGAGTGCC GTAGATGCCA CATTAAATGT 
CATAAAGATC ACATGGACAA AAAGGAAGAG ATCATAGCCC CCTGCAAAGT TTATTATGAT 
ATATCATCGG CAAAGAATCT GTTGTTATTA GCAAATTCAA CGGAAGAACA GCAGAAATGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCTCCAG CTCCAGACCC TTTTGCCCGA 
TCATCCCCTA GAACCTCAAT GAAAATACAA CAAAACCAGT CCATTCGGCG GCCCAGTCGA 
CAGCTTGCTC CAAACAAACC AAGATTGCTT GATTTGGCAT TTAAAGACTG GGATTGGTCA 
TTTGATGATG TTGATGGTGA TGATGATGAT GACGATGTTT TTGATTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239971.1
CDS	1..4251
Translation	MSRPPPTGKMPGAPEAAPGDGAGAGRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITFRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQLQKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRISGLEEDLKTGKTLLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQSQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDTQEQLSKLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGMDSSSIGSGPGDAEPDDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRLLDLAFKDWDWSFDDVDGDDDDDDVFDF

Target ORF information:

RefSeq Version	XM_006239909.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239909.3

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGGCCGC GCCGGGGGAC 
GGGGCGGGCG CGGGTCGTCA GAGGAAGCTG GAGGCGCTCA TCCGAGACCC TCGCTCGCCC 
ATCAACGTGG AAAGCCTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAATAAAAA TATAGATAAT TTCCTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTTTACAGAT GAAAGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTTCA GTTGGTTCGT CATAAGGCAT CACAGAAGGT TTATGCAATG 
AAGCTTCTTA GTAAATTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAAGAA 
AGAGATATTA TGGCTTTTGC TAACAGTCCG TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGACAGGT ATCTGTACAT GGTGATGGAG TACATGCCAG GTGGAGACCT TGTTAACCTG 
ATGAGCAATT ATGATGTGCC TGAGAAGTGG GCCAAGTTTT ATACTGCTGA AGTCGTGCTT 
GCACTGGATG CAATACACTC CATGGGCTTA ATACACAGAG ATGTGAAGCC CGATAACATG 
CTATTGGATA AACACGGACA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAAATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACTC CAGACTACAT ATCACCTGAG 
GTTCTGAAAT CACAAGGAGG TGATGGTTAC TATGGACGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTTTGAGAT GTTAGTAGGG GACACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACCTACA GCAAAATTAT GGATCATAAA AATTCACTGT GTTTTCCTGA AGATACAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATATGT GCCTTCTTAA CAGACAGGGA GGTACGACTT 
GGAAGAAATG GGGTAGAAGA AATCAAACAG CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC GGCAGCTCCA GTGGTGCCTG AGCTCAGCAG TGACATAGAC 
AGCAGCAACT TTGATGACAT TGAGGATGAC AAAGGCGATG TAGAGACCTT CCCGATTCCT 
AAAGCTTTTG TGGGAAATCA GCTGCCTTTT ATAGGATTTA CCTACTTTAG AGAAAATTTG 
TTACTAAGTG ATTCTCCACC TTGTAGAGAA AATGATGCAA TACAAACAAG GAAAAGTGAA 
GAAAGTCAGG AGATTCAGAA AAAATTATAT GCACTAGAAG AACACCTTAG CAGTGAGGTA 
CAAGCCAAGG AGGAACTGGA ACAGAAGTGC AAGTCTATTA ATACTCGTCT AGAGAAAACA 
GCAAAGGAAC TAGAAGAAGA GATTACCTTT AGGAAAAATG TGGAATCAAC ATTAAGACAG 
CTAGAAAGAG AAAAGGCCCT TCTTCAGCAC AAAAATGCAG AATATCAACG GAAAGCTGAT 
CATGAAGCTG ACAAGAAACG GAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATCTGA AGAAAAGAAA CCAGAGCTCT CAGATATCCA CTGAGAAGGT CAATCAGCTC 
CAGAAACAAC TGGATGAAGC TAATGCTTTG CTAAGAACAG AGTCTGATAC TGCAGCACGG 
TTAAGAAAAA CCCAGGCAGA AAGTTCCAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTTGAA ACTGCCAAGT TAAAACTTGA GAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGAAGGGACC GGACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATCTGGG TTGGAAGAAG ATTTGAAGAC TGGCAAAACC 
TTACTAGCAA AAGTAGAGCT GGAGAAGAGA CAGCTGCAGG AGAAGCTCAC TGACTTAGAG 
AAGGAAAAGA GCAACATGGA AATAGATATG ACATATCAAC TGAAAGTTAT ACAGCAGAGT 
TTAGAACAAG AAGAAGCTGA ACACAAGACC ACAAAAGCAC GACTAGCGGA TAAAAATAAG 
ATCTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTG 
TTGGAAGAGA GGTCTTTAAA GCAGAAAGTG GAGAACCTAC TCCTGGAAGC CGAGAAGAGA 
TGTTCTATAT TGGATTGTGA CCTCAAACAG TCTCAGCAGA AACTGAATGA GCTGCTGAAG 
CAGAAAGATG TGCTGAATGA GGATGTTAGA AATTTGACAT TAAAAATAGA GCAGGAAACT 
CAGAAGCGTT GTCTTATGCA AAATGACCTG AAGATGCAGA CCCAGCAAGT TAACACCCTA 
AAAATGTCAG AAAAGCAAAT AAAACAAGAA AATAATCATC TTATGGAAAT GAAAATGAAC 
TTGGAAAAGC AAAATGCTGA ACTTCGAAAA GAGCGGCAGG ATGCAGATGG GCAGATGAAG 
GAACTGCAAG ACCAACTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAAGTT 
AGAGAACTTA AAGAAGAAAA TGAAGAGAAA ACTAAACTTT GTAAAGAGTT GCAGCAGAAA 
AAGCAGGATT TACAGGATGA AAGAGACTCC TTGGCTGCCC AGCTGGAGAT CACCCTCACC 
AAAGCAGACT CTGAGCAGCT GGCTCGCTCC ATTGCTGAGG AGCAATACTC TGACTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAAATC AAAGAGATGA TGGCTAGACA CAAACAAGAG 
CTTACTGAAA AGGACGCTAC AATTGCATCC CTTGAAGAAA CAAATAGAAC ACTAACTAGT 
GATGTTGCAA ATCTTGCAAA CGAGAAAGAA GAACTAAACA ACAAGTTGAA AGATACCCAA 
GAACAACTGT CAAAGTTGAA AGATGAAGAG ATCAGTGCAG CGGCTATTAA AGCACAGTTT 
GAGAAGCAGC TGCTGACTGA GCGAACACTC AAGACTCAAG CTGTGAATAA GTTGGCAGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAGTGACA CAGATGTGCG AAGAAAAGAA 
AAGGAAAACA GAAAATTACA TATGGAGCTT AAGTCTGAAC GTGAAAAATT GACACAACAG 
ATGATCAAAT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGCCAA 
ATTCGAATAG AACTGCAGAT GACCCTGGAC AGTAAAGACA GTGACATTGA ACAGCTTCGG 
TCGCAGCTCC AGGCCTTGCA TATTGGTATG GACAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGC CTGATGACGG ATTTCCAGAA TCACGATTAG AAGGATGGCT GTCGTTGCCT 
GTGCGGAACA ACACTAAAAA GTTTGGATGG GTTAAGAAGT ATGTGATTGT AAGCAGCAAG 
AAGATTCTTT TCTATGATAG TGAACAAGAT AAAGAGCAGT CTAACCCTTA CATGGTGTTA 
GACATAGACA AGCTATTTCA TGTCCGACCT GTTACTCAGA CAGATGTATA CAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTTCAGATC CTGTATGCCA ATGAAGGAGA AAGTAAAAAG 
GAACCAGAAT TTCCAGTGGA ACCAGTGGGA GAAAAATCTA ATTATATTTG CCACAAAGGA 
CATGAGTTTA TTCCTACTCT TTATCACTTC CCAACCAACT GTGAGGCCTG TATGAAGCCA 
CTGTGGCACA TGTTTAAACC TCCTCCTGCT CTAGAGTGCC GTAGATGCCA CATTAAATGT 
CATAAAGATC ACATGGACAA AAAGGAAGAG ATCATAGCCC CCTGCAAAGT TTATTATGAT 
ATATCATCGG CAAAGAATCT GTTGTTATTA GCAAATTCAA CGGAAGAACA GCAGAAATGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCTCCAG CTCCAGACCC TTTTGCCCGA 
TCATCCCCTA GAACCTCAAT GAAAATACAA CAAAACCAGT CCATTCGGCG GCCCAGTCGA 
CAGCTTGCTC CAAACAAACC AAGATTGCTT GATTTGGCAT TTAAAGACTG GGATTGGTCA 
TTTGATGATG TTGATGGTGA TGATGATGAT GACGATGTTT TTGATTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rock2 ( XM_008764574.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762796.1 Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X1, mRNA.

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CloneID	ORa43135
Clone ID Related Accession (Same CDS sequence)	XM_008764574.1
Accession Version	XM_008764574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGGCCGC GCCGGGGGAC 
GGGGCGGGCG CGGGTCGTCA GAGGAAGCTG GAGGCGCTCA TCCGAGACCC TCGCTCGCCC 
ATCAACGTGG AAAGCCTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAATAAAAA TATAGATAAT TTCCTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTTTACAGAT GAAAGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTTCA GTTGGTTCGT CATAAGGCAT CACAGAAGGT TTATGCAATG 
AAGCTTCTTA GTAAATTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAAGAA 
AGAGATATTA TGGCTTTTGC TAACAGTCCG TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGACAGGT ATCTGTACAT GGTGATGGAG TACATGCCAG GTGGAGACCT TGTTAACCTG 
ATGAGCAATT ATGATGTGCC TGAGAAGTGG GCCAAGTTTT ATACTGCTGA AGTCGTGCTT 
GCACTGGATG CAATACACTC CATGGGCTTA ATACACAGAG ATGTGAAGCC CGATAACATG 
CTATTGGATA AACACGGACA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAAATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACTC CAGACTACAT ATCACCTGAG 
GTTCTGAAAT CACAAGGAGG TGATGGTTAC TATGGACGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTTTGAGAT GTTAGTAGGG GACACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACCTACA GCAAAATTAT GGATCATAAA AATTCACTGT GTTTTCCTGA AGATACAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATATGT GCCTTCTTAA CAGACAGGGA GGTACGACTT 
GGAAGAAATG GGGTAGAAGA AATCAAACAG CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC GGCAGCTCCA GTGGTGCCTG AGCTCAGCAG TGACATAGAC 
AGCAGCAACT TTGATGACAT TGAGGATGAC AAAGGCGATG TAGAGACCTT CCCGATTCCT 
AAAGCTTTTG TGGGAAATCA GCTGCCTTTT ATAGGATTTA CCTACTTTAG AGAAAATTTG 
TTACTAAGTG ATTCTCCACC TTGTAGAGAA AATGATGCAA TACAAACAAG GAAAAGTGAA 
GAAAGTCAGG AGATTCAGAA AAAATTATAT GCACTAGAAG AACACCTTAG CAGTGAGGTA 
CAAGCCAAGG AGGAACTGGA ACAGAAGTGC AAGTCTATTA ATACTCGTCT AGAGAAAACA 
GCAAAGGAAC TAGAAGAAGA GATTACCTTT AGGAAAAATG TGGAATCAAC ATTAAGACAG 
CTAGAAAGAG AAAAGGCCCT TCTTCAGCAC AAAAATGCAG AATATCAACG GAAAGCTGAT 
CATGAAGCTG ACAAGAAACG GAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATCTGA AGAAAAGAAA CCAGAGCTCT CAGATATCCA CTGAGAAGGT CAATCAGCTC 
CAGAAACAAC TGGATGAAGC TAATGCTTTG CTAAGAACAG AGTCTGATAC TGCAGCACGG 
TTAAGAAAAA CCCAGGCAGA AAGTTCCAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTTGAA ACTGCCAAGT TAAAACTTGA GAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGAAGGGACC GGACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATCTGGG TTGGAAGAAG ATTTGAAGAC TGGCAAAACC 
TTACTAGCAA AAGTAGAGCT GGAGAAGAGA CAGCTGCAGG AGAAGCTCAC TGACTTAGAG 
AAGGAAAAGA GCAACATGGA AATAGATATG ACATATCAAC TGAAAGTTAT ACAGCAGAGT 
TTAGAACAAG AAGAAGCTGA ACACAAGACC ACAAAAGCAC GACTAGCGGA TAAAAATAAG 
ATCTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTG 
TTGGAAGAGA GGTCTTTAAA GCAGAAAGTG GAGAACCTAC TCCTGGAAGC CGAGAAGAGA 
TGTTCTATAT TGGATTGTGA CCTCAAACAG TCTCAGCAGA AACTGAATGA GCTGCTGAAG 
CAGAAAGATG TGCTGAATGA GGATGTTAGA AATTTGACAT TAAAAATAGA GCAGGAAACT 
CAGAAGCGTT GTCTTATGCA AAATGACCTG AAGATGCAGA CCCAGCAAGT TAACACCCTA 
AAAATGTCAG AAAAGCAAAT AAAACAAGAA AATAATCATC TTATGGAAAT GAAAATGAAC 
TTGGAAAAGC AAAATGCTGA ACTTCGAAAA GAGCGGCAGG ATGCAGATGG GCAGATGAAG 
GAACTGCAAG ACCAACTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAAGTT 
AGAGAACTTA AAGAAGAAAA TGAAGAGAAA ACTAAACTTT GTAAAGAGTT GCAGCAGAAA 
AAGCAGGATT TACAGGATGA AAGAGACTCC TTGGCTGCCC AGCTGGAGAT CACCCTCACC 
AAAGCAGACT CTGAGCAGCT GGCTCGCTCC ATTGCTGAGG AGCAATACTC TGACTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAAATC AAAGAGATGA TGGCTAGACA CAAACAAGAG 
CTTACTGAAA AGGACGCTAC AATTGCATCC CTTGAAGAAA CAAATAGAAC ACTAACTAGT 
GATGTTGCAA ATCTTGCAAA CGAGAAAGAA GAACTAAACA ACAAGTTGAA AGATACCCAA 
GAACAACTGT CAAAGTTGAA AGATGAAGAG ATCAGTGCAG CGGCTATTAA AGCACAGTTT 
GAGAAGCAGC TGCTGACTGA GCGAACACTC AAGACTCAAG CTGTGAATAA GTTGGCAGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAGTGACA CAGATGTGCG AAGAAAAGAA 
AAGGAAAACA GAAAATTACA TATGGAGCTT AAGTCTGAAC GTGAAAAATT GACACAACAG 
ATGATCAAAT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGCCAA 
ATTCGAATAG AACTGCAGAT GACCCTGGAC AGTAAAGACA GTGACATTGA ACAGCTTCGG 
TCGCAGCTCC AGGCCTTGCA TATTGGTATG GACAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGC CTGATGACGG ATTTCCAGTC CATATCACTC AGTCACACAC TATGGAATCC 
ATGTCCTTCA CCTACCAGCG GTCCTCTACC TCTCTCAATA TTGCCACTAA GCCCTCCAGT 
TCTCACATGC TTCTTGACTC TGACTCTGAA GAAGATTCCT TGCCTTACTT ACCTAGTTCA 
TCGGAGCCTA TTAGTACAGA ATCACGATTA GAAGGATGGC TGTCGTTGCC TGTGCGGAAC 
AACACTAAAA AGTTTGGATG GGTTAAGAAG TATGTGATTG TAAGCAGCAA GAAGATTCTT 
TTCTATGATA GTGAACAAGA TAAAGAGCAG TCTAACCCTT ACATGGTGTT AGACATAGAC 
AAGCTATTTC ATGTCCGACC TGTTACTCAG ACAGATGTAT ACAGAGCAGA TGCTAAAGAA 
ATTCCAAGGA TATTTCAGAT CCTGTATGCC AATGAAGGAG AAAGTAAAAA GGAACCAGAA 
TTTCCAGTGG AACCAGTGGG AGAAAAATCT AATTATATTT GCCACAAAGG ACATGAGTTT 
ATTCCTACTC TTTATCACTT CCCAACCAAC TGTGAGGCCT GTATGAAGCC ACTGTGGCAC 
ATGTTTAAAC CTCCTCCTGC TCTAGAGTGC CGTAGATGCC ACATTAAATG TCATAAAGAT 
CACATGGACA AAAAGGAAGA GATCATAGCC CCCTGCAAAG TTTATTATGA TATATCATCG 
GCAAAGAATC TGTTGTTATT AGCAAATTCA ACGGAAGAAC AGCAGAAATG GGTTAGTCGG 
TTGGTGAAAA AGATACCTAA AAAGCCTCCA GCTCCAGACC CTTTTGCCCG ATCATCCCCT 
AGAACCTCAA TGAAAATACA ACAAAACCAG TCCATTCGGC GGCCCAGTCG ACAGCTTGCT 
CCAAACAAAC CAAGATTGCT TGATTTGGCA TTTAAAGACT GGGATTGGTC ATTTGATGAT 
GTTGATGGTG ATGATGATGA TGACGATGTT TTTGATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762796.1
CDS	79..4500
Translation	MSRPPPTGKMPGAPEAAPGDGAGAGRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITFRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQLQKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRISGLEEDLKTGKTLLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQSQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDTQEQLSKLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGMDSSSIGSGPGDAEPDDGFPVHITQSHTMESMSFTYQRSSTSLNIATKPSSSHMLLDSDSEEDSLPYLPSSSEPISTESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRLLDLAFKDWDWSFDDVDGDDDDDDVFDF

Target ORF information:

RefSeq Version	XM_008764574.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764574.1

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGGCCGC GCCGGGGGAC 
GGGGCGGGCG CGGGTCGTCA GAGGAAGCTG GAGGCGCTCA TCCGAGACCC TCGCTCGCCC 
ATCAACGTGG AAAGCCTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAATAAAAA TATAGATAAT TTCCTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTTTACAGAT GAAAGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTTCA GTTGGTTCGT CATAAGGCAT CACAGAAGGT TTATGCAATG 
AAGCTTCTTA GTAAATTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAAGAA 
AGAGATATTA TGGCTTTTGC TAACAGTCCG TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGACAGGT ATCTGTACAT GGTGATGGAG TACATGCCAG GTGGAGACCT TGTTAACCTG 
ATGAGCAATT ATGATGTGCC TGAGAAGTGG GCCAAGTTTT ATACTGCTGA AGTCGTGCTT 
GCACTGGATG CAATACACTC CATGGGCTTA ATACACAGAG ATGTGAAGCC CGATAACATG 
CTATTGGATA AACACGGACA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAAATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACTC CAGACTACAT ATCACCTGAG 
GTTCTGAAAT CACAAGGAGG TGATGGTTAC TATGGACGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTTTGAGAT GTTAGTAGGG GACACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACCTACA GCAAAATTAT GGATCATAAA AATTCACTGT GTTTTCCTGA AGATACAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATATGT GCCTTCTTAA CAGACAGGGA GGTACGACTT 
GGAAGAAATG GGGTAGAAGA AATCAAACAG CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC GGCAGCTCCA GTGGTGCCTG AGCTCAGCAG TGACATAGAC 
AGCAGCAACT TTGATGACAT TGAGGATGAC AAAGGCGATG TAGAGACCTT CCCGATTCCT 
AAAGCTTTTG TGGGAAATCA GCTGCCTTTT ATAGGATTTA CCTACTTTAG AGAAAATTTG 
TTACTAAGTG ATTCTCCACC TTGTAGAGAA AATGATGCAA TACAAACAAG GAAAAGTGAA 
GAAAGTCAGG AGATTCAGAA AAAATTATAT GCACTAGAAG AACACCTTAG CAGTGAGGTA 
CAAGCCAAGG AGGAACTGGA ACAGAAGTGC AAGTCTATTA ATACTCGTCT AGAGAAAACA 
GCAAAGGAAC TAGAAGAAGA GATTACCTTT AGGAAAAATG TGGAATCAAC ATTAAGACAG 
CTAGAAAGAG AAAAGGCCCT TCTTCAGCAC AAAAATGCAG AATATCAACG GAAAGCTGAT 
CATGAAGCTG ACAAGAAACG GAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATCTGA AGAAAAGAAA CCAGAGCTCT CAGATATCCA CTGAGAAGGT CAATCAGCTC 
CAGAAACAAC TGGATGAAGC TAATGCTTTG CTAAGAACAG AGTCTGATAC TGCAGCACGG 
TTAAGAAAAA CCCAGGCAGA AAGTTCCAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTTGAA ACTGCCAAGT TAAAACTTGA GAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGAAGGGACC GGACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATCTGGG TTGGAAGAAG ATTTGAAGAC TGGCAAAACC 
TTACTAGCAA AAGTAGAGCT GGAGAAGAGA CAGCTGCAGG AGAAGCTCAC TGACTTAGAG 
AAGGAAAAGA GCAACATGGA AATAGATATG ACATATCAAC TGAAAGTTAT ACAGCAGAGT 
TTAGAACAAG AAGAAGCTGA ACACAAGACC ACAAAAGCAC GACTAGCGGA TAAAAATAAG 
ATCTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTG 
TTGGAAGAGA GGTCTTTAAA GCAGAAAGTG GAGAACCTAC TCCTGGAAGC CGAGAAGAGA 
TGTTCTATAT TGGATTGTGA CCTCAAACAG TCTCAGCAGA AACTGAATGA GCTGCTGAAG 
CAGAAAGATG TGCTGAATGA GGATGTTAGA AATTTGACAT TAAAAATAGA GCAGGAAACT 
CAGAAGCGTT GTCTTATGCA AAATGACCTG AAGATGCAGA CCCAGCAAGT TAACACCCTA 
AAAATGTCAG AAAAGCAAAT AAAACAAGAA AATAATCATC TTATGGAAAT GAAAATGAAC 
TTGGAAAAGC AAAATGCTGA ACTTCGAAAA GAGCGGCAGG ATGCAGATGG GCAGATGAAG 
GAACTGCAAG ACCAACTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAAGTT 
AGAGAACTTA AAGAAGAAAA TGAAGAGAAA ACTAAACTTT GTAAAGAGTT GCAGCAGAAA 
AAGCAGGATT TACAGGATGA AAGAGACTCC TTGGCTGCCC AGCTGGAGAT CACCCTCACC 
AAAGCAGACT CTGAGCAGCT GGCTCGCTCC ATTGCTGAGG AGCAATACTC TGACTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAAATC AAAGAGATGA TGGCTAGACA CAAACAAGAG 
CTTACTGAAA AGGACGCTAC AATTGCATCC CTTGAAGAAA CAAATAGAAC ACTAACTAGT 
GATGTTGCAA ATCTTGCAAA CGAGAAAGAA GAACTAAACA ACAAGTTGAA AGATACCCAA 
GAACAACTGT CAAAGTTGAA AGATGAAGAG ATCAGTGCAG CGGCTATTAA AGCACAGTTT 
GAGAAGCAGC TGCTGACTGA GCGAACACTC AAGACTCAAG CTGTGAATAA GTTGGCAGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAGTGACA CAGATGTGCG AAGAAAAGAA 
AAGGAAAACA GAAAATTACA TATGGAGCTT AAGTCTGAAC GTGAAAAATT GACACAACAG 
ATGATCAAAT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGCCAA 
ATTCGAATAG AACTGCAGAT GACCCTGGAC AGTAAAGACA GTGACATTGA ACAGCTTCGG 
TCGCAGCTCC AGGCCTTGCA TATTGGTATG GACAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGC CTGATGACGG ATTTCCAGTC CATATCACTC AGTCACACAC TATGGAATCC 
ATGTCCTTCA CCTACCAGCG GTCCTCTACC TCTCTCAATA TTGCCACTAA GCCCTCCAGT 
TCTCACATGC TTCTTGACTC TGACTCTGAA GAAGATTCCT TGCCTTACTT ACCTAGTTCA 
TCGGAGCCTA TTAGTACAGA ATCACGATTA GAAGGATGGC TGTCGTTGCC TGTGCGGAAC 
AACACTAAAA AGTTTGGATG GGTTAAGAAG TATGTGATTG TAAGCAGCAA GAAGATTCTT 
TTCTATGATA GTGAACAAGA TAAAGAGCAG TCTAACCCTT ACATGGTGTT AGACATAGAC 
AAGCTATTTC ATGTCCGACC TGTTACTCAG ACAGATGTAT ACAGAGCAGA TGCTAAAGAA 
ATTCCAAGGA TATTTCAGAT CCTGTATGCC AATGAAGGAG AAAGTAAAAA GGAACCAGAA 
TTTCCAGTGG AACCAGTGGG AGAAAAATCT AATTATATTT GCCACAAAGG ACATGAGTTT 
ATTCCTACTC TTTATCACTT CCCAACCAAC TGTGAGGCCT GTATGAAGCC ACTGTGGCAC 
ATGTTTAAAC CTCCTCCTGC TCTAGAGTGC CGTAGATGCC ACATTAAATG TCATAAAGAT 
CACATGGACA AAAAGGAAGA GATCATAGCC CCCTGCAAAG TTTATTATGA TATATCATCG 
GCAAAGAATC TGTTGTTATT AGCAAATTCA ACGGAAGAAC AGCAGAAATG GGTTAGTCGG 
TTGGTGAAAA AGATACCTAA AAAGCCTCCA GCTCCAGACC CTTTTGCCCG ATCATCCCCT 
AGAACCTCAA TGAAAATACA ACAAAACCAG TCCATTCGGC GGCCCAGTCG ACAGCTTGCT 
CCAAACAAAC CAAGATTGCT TGATTTGGCA TTTAAAGACT GGGATTGGTC ATTTGATGAT 
GTTGATGGTG ATGATGATGA TGACGATGTT TTTGATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rock2 ( XM_008764575.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762797.1 Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X2, mRNA.

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CloneID	ORa43136
Clone ID Related Accession (Same CDS sequence)	XM_008764575.2
Accession Version	XM_008764575.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	rho-associated protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGGCCGC GCCGGGGGAC 
GGGGCGGGCG CGGGTCGTCA GAGGAAGCTG GAGGCGCTCA TCCGAGACCC TCGCTCGCCC 
ATCAACGTGG AAAGCCTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAATAAAAA TATAGATAAT TTCCTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTTTACAGAT GAAAGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTTCA GTTGGTTCGT CATAAGGCAT CACAGAAGGT TTATGCAATG 
AAGCTTCTTA GTAAATTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAAGAA 
AGAGATATTA TGGCTTTTGC TAACAGTCCG TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGACAGGT ATCTGTACAT GGTGATGGAG TACATGCCAG GTGGAGACCT TGTTAACCTG 
ATGAGCAATT ATGATGTGCC TGAGAAGTGG GCCAAGTTTT ATACTGCTGA AGTCGTGCTT 
GCACTGGATG CAATACACTC CATGGGCTTA ATACACAGAG ATGTGAAGCC CGATAACATG 
CTATTGGATA AACACGGACA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAAATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACTC CAGACTACAT ATCACCTGAG 
GTTCTGAAAT CACAAGGAGG TGATGGTTAC TATGGACGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTTTGAGAT GTTAGTAGGG GACACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACCTACA GCAAAATTAT GGATCATAAA AATTCACTGT GTTTTCCTGA AGATACAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATATGT GCCTTCTTAA CAGACAGGGA GGTACGACTT 
GGAAGAAATG GGGTAGAAGA AATCAAACAG CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC GGCAGCTCCA GTGGTGCCTG AGCTCAGCAG TGACATAGAC 
AGCAGCAACT TTGATGACAT TGAGGATGAC AAAGGCGATG TAGAGACCTT CCCGATTCCT 
AAAGCTTTTG TGGGAAATCA GCTGCCTTTT ATAGGATTTA CCTACTTTAG AGAAAATTTG 
TTACTAAGTG ATTCTCCACC TTGTAGAGAA AATGATGCAA TACAAACAAG GAAAAGTGAA 
GAAAGTCAGG AGATTCAGAA AAAATTATAT GCACTAGAAG AACACCTTAG CAGTGAGGTA 
CAAGCCAAGG AGGAACTGGA ACAGAAGTGC AAGTCTATTA ATACTCGTCT AGAGAAAACA 
GCAAAGGAAC TAGAAGAAGA GATTACCTTT AGGAAAAATG TGGAATCAAC ATTAAGACAG 
CTAGAAAGAG AAAAGGCCCT TCTTCAGCAC AAAAATGCAG AATATCAACG GAAAGCTGAT 
CATGAAGCTG ACAAGAAACG GAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATCTGA AGAAAAGAAA CCAGAGCTCT CAGATATCCA CTGAGAAGGT CAATCAGCTC 
CAGAAACAAC TGGATGAAGC TAATGCTTTG CTAAGAACAG AGTCTGATAC TGCAGCACGG 
TTAAGAAAAA CCCAGGCAGA AAGTTCCAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTTGAA ACTGCCAAGT TAAAACTTGA GAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGAAGGGACC GGACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATCTGGG TTGGAAGAAG ATTTGAAGAC TGGCAAAACC 
TTACTAGCAA AAGTAGAGCT GGAGAAGAGA CAGCTGCAGG AGAAGCTCAC TGACTTAGAG 
AAGGAAAAGA GCAACATGGA AATAGATATG ACATATCAAC TGAAAGTTAT ACAGCAGAGT 
TTAGAACAAG AAGAAGCTGA ACACAAGACC ACAAAAGCAC GACTAGCGGA TAAAAATAAG 
ATCTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTG 
TTGGAAGAGA GGTCTTTAAA GCAGAAAGTG GAGAACCTAC TCCTGGAAGC CGAGAAGAGA 
TGTTCTATAT TGGATTGTGA CCTCAAACAG TCTCAGCAGA AACTGAATGA GCTGCTGAAG 
CAGAAAGATG TGCTGAATGA GGATGTTAGA AATTTGACAT TAAAAATAGA GCAGGAAACT 
CAGAAGCGTT GTCTTATGCA AAATGACCTG AAGATGCAGA CCCAGCAAGT TAACACCCTA 
AAAATGTCAG AAAAGCAAAT AAAACAAGAA AATAATCATC TTATGGAAAT GAAAATGAAC 
TTGGAAAAGC AAAATGCTGA ACTTCGAAAA GAGCGGCAGG ATGCAGATGG GCAGATGAAG 
GAACTGCAAG ACCAACTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAAGTT 
AGAGAACTTA AAGAAGAAAA TGAAGAGAAA ACTAAACTTT GTAAAGAGTT GCAGCAGAAA 
AAGCAGGATT TACAGGATGA AAGAGACTCC TTGGCTGCCC AGCTGGAGAT CACCCTCACC 
AAAGCAGACT CTGAGCAGCT GGCTCGCTCC ATTGCTGAGG AGCAATACTC TGACTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAAATC AAAGAGATGA TGGCTAGACA CAAACAAGAG 
CTTACTGAAA AGGACGCTAC AATTGCATCC CTTGAAGAAA CAAATAGAAC ACTAACTAGT 
GATGTTGCAA ATCTTGCAAA CGAGAAAGAA GAACTAAACA ACAAGTTGAA AGATACCCAA 
GAACAACTGT CAAAGTTGAA AGATGAAGAG ATCAGTGCAG CGGCTATTAA AGCACAGTTT 
GAGAAGCAGC TGCTGACTGA GCGAACACTC AAGACTCAAG CTGTGAATAA GTTGGCAGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAGTGACA CAGATGTGCG AAGAAAAGAA 
AAGGAAAACA GAAAATTACA TATGGAGCTT AAGTCTGAAC GTGAAAAATT GACACAACAG 
ATGATCAAAT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGCCAA 
ATTCGAATAG AACTGCAGAT GACCCTGGAC AGTAAAGACA GTGACATTGA ACAGCTTCGG 
TCGCAGCTCC AGGCCTTGCA TATTGGTATG GACAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGC CTGATGACGG ATTTCCAGTC CATATCACTC AGTCACACAC TATGGAATCC 
ATGTCCTTCA CCTACCAGCG GTCCTCTACC TCTCTCAATA TTGCCACTAA GCCCTCCAGT 
TCTCACATGC TTCTTGACTC TGACTCTGAA GAAGATTCCT TGCCTTACTT ACCTAGTTCA 
TCGGAGCCTA TTAGTACAGA ATCACGATTA GAAGGATGGC TGTCGTTGCC TGTGCGGAAC 
AACACTAAAA AGTTTGGATG GGTTAAGAAG TATGTGATTG TAAGCAGCAA GAAGATTCTT 
TTCTATGATA GTGAACAAGA TAAAGAGCAG TCTAACCCTT ACATGGTGTT AGACATAGAC 
AAGCTATTTC ATGTCCGACC TGTTACTCAG ACAGATGTAT ACAGAGCAGA TGCTAAAGAA 
ATTCCAAGGA TATTTCAGAT CCTGTATGCC AATGAAGGAG AAAGTAAAAA GGAACCAGAA 
TTTCCAGTGG AACCAGTGGG AGAAAAATCT AATTATATTT GCCACAAAGG ACATGAGTTT 
ATTCCTACTC TTTATCACTT CCCAACCAAC TGTGAGGCCT GTATGAAGCC ACTGTGGCAC 
ATGTTTAAAC CTCCTCCTGC TCTAGAGTGC CGTAGATGCC ACATTAAATG TCATAAAGAT 
CACATGGACA AAAAGGAAGA GATCATAGCC CCCTGCAAAG TTTATTATGA TATATCATCG 
GCAAAGAATC TGTTGTTATT AGCAAATTCA ACGGAAGAAC AGCAGAAATG GGTTAGTCGG 
TTGGTGAAAA AGATACCTAA AAAGCCTCCA GCTCCAGACC CTTTTGCCCG ATCATCCCCT 
AGAACCTCAA TGAAAATACA ACAAAACCAG TCCATTCGGC GGCCCAGTCG ACAGCTTGCT 
CCAAACAAAC CAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762797.1
CDS	1..4338
Translation	MSRPPPTGKMPGAPEAAPGDGAGAGRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITFRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQLQKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRISGLEEDLKTGKTLLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQSQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKDTQEQLSKLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGMDSSSIGSGPGDAEPDDGFPVHITQSHTMESMSFTYQRSSTSLNIATKPSSSHMLLDSDSEEDSLPYLPSSSEPISTESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	XM_008764575.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764575.2

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGGCCGC GCCGGGGGAC 
GGGGCGGGCG CGGGTCGTCA GAGGAAGCTG GAGGCGCTCA TCCGAGACCC TCGCTCGCCC 
ATCAACGTGG AAAGCCTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAATAAAAA TATAGATAAT TTCCTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTTTACAGAT GAAAGCAGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTTCA GTTGGTTCGT CATAAGGCAT CACAGAAGGT TTATGCAATG 
AAGCTTCTTA GTAAATTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAAGAA 
AGAGATATTA TGGCTTTTGC TAACAGTCCG TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGACAGGT ATCTGTACAT GGTGATGGAG TACATGCCAG GTGGAGACCT TGTTAACCTG 
ATGAGCAATT ATGATGTGCC TGAGAAGTGG GCCAAGTTTT ATACTGCTGA AGTCGTGCTT 
GCACTGGATG CAATACACTC CATGGGCTTA ATACACAGAG ATGTGAAGCC CGATAACATG 
CTATTGGATA AACACGGACA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAAATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACTC CAGACTACAT ATCACCTGAG 
GTTCTGAAAT CACAAGGAGG TGATGGTTAC TATGGACGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTTTGAGAT GTTAGTAGGG GACACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACCTACA GCAAAATTAT GGATCATAAA AATTCACTGT GTTTTCCTGA AGATACAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATATGT GCCTTCTTAA CAGACAGGGA GGTACGACTT 
GGAAGAAATG GGGTAGAAGA AATCAAACAG CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC GGCAGCTCCA GTGGTGCCTG AGCTCAGCAG TGACATAGAC 
AGCAGCAACT TTGATGACAT TGAGGATGAC AAAGGCGATG TAGAGACCTT CCCGATTCCT 
AAAGCTTTTG TGGGAAATCA GCTGCCTTTT ATAGGATTTA CCTACTTTAG AGAAAATTTG 
TTACTAAGTG ATTCTCCACC TTGTAGAGAA AATGATGCAA TACAAACAAG GAAAAGTGAA 
GAAAGTCAGG AGATTCAGAA AAAATTATAT GCACTAGAAG AACACCTTAG CAGTGAGGTA 
CAAGCCAAGG AGGAACTGGA ACAGAAGTGC AAGTCTATTA ATACTCGTCT AGAGAAAACA 
GCAAAGGAAC TAGAAGAAGA GATTACCTTT AGGAAAAATG TGGAATCAAC ATTAAGACAG 
CTAGAAAGAG AAAAGGCCCT TCTTCAGCAC AAAAATGCAG AATATCAACG GAAAGCTGAT 
CATGAAGCTG ACAAGAAACG GAATTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATCTGA AGAAAAGAAA CCAGAGCTCT CAGATATCCA CTGAGAAGGT CAATCAGCTC 
CAGAAACAAC TGGATGAAGC TAATGCTTTG CTAAGAACAG AGTCTGATAC TGCAGCACGG 
TTAAGAAAAA CCCAGGCAGA AAGTTCCAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTTGAA ACTGCCAAGT TAAAACTTGA GAAGGAATTT 
ATCAATCTTC AGTCAGCTCT AGAATCTGAA AGAAGGGACC GGACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATCTGGG TTGGAAGAAG ATTTGAAGAC TGGCAAAACC 
TTACTAGCAA AAGTAGAGCT GGAGAAGAGA CAGCTGCAGG AGAAGCTCAC TGACTTAGAG 
AAGGAAAAGA GCAACATGGA AATAGATATG ACATATCAAC TGAAAGTTAT ACAGCAGAGT 
TTAGAACAAG AAGAAGCTGA ACACAAGACC ACAAAAGCAC GACTAGCGGA TAAAAATAAG 
ATCTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTG 
TTGGAAGAGA GGTCTTTAAA GCAGAAAGTG GAGAACCTAC TCCTGGAAGC CGAGAAGAGA 
TGTTCTATAT TGGATTGTGA CCTCAAACAG TCTCAGCAGA AACTGAATGA GCTGCTGAAG 
CAGAAAGATG TGCTGAATGA GGATGTTAGA AATTTGACAT TAAAAATAGA GCAGGAAACT 
CAGAAGCGTT GTCTTATGCA AAATGACCTG AAGATGCAGA CCCAGCAAGT TAACACCCTA 
AAAATGTCAG AAAAGCAAAT AAAACAAGAA AATAATCATC TTATGGAAAT GAAAATGAAC 
TTGGAAAAGC AAAATGCTGA ACTTCGAAAA GAGCGGCAGG ATGCAGATGG GCAGATGAAG 
GAACTGCAAG ACCAACTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAAGTT 
AGAGAACTTA AAGAAGAAAA TGAAGAGAAA ACTAAACTTT GTAAAGAGTT GCAGCAGAAA 
AAGCAGGATT TACAGGATGA AAGAGACTCC TTGGCTGCCC AGCTGGAGAT CACCCTCACC 
AAAGCAGACT CTGAGCAGCT GGCTCGCTCC ATTGCTGAGG AGCAATACTC TGACTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAAATC AAAGAGATGA TGGCTAGACA CAAACAAGAG 
CTTACTGAAA AGGACGCTAC AATTGCATCC CTTGAAGAAA CAAATAGAAC ACTAACTAGT 
GATGTTGCAA ATCTTGCAAA CGAGAAAGAA GAACTAAACA ACAAGTTGAA AGATACCCAA 
GAACAACTGT CAAAGTTGAA AGATGAAGAG ATCAGTGCAG CGGCTATTAA AGCACAGTTT 
GAGAAGCAGC TGCTGACTGA GCGAACACTC AAGACTCAAG CTGTGAATAA GTTGGCAGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAGTGACA CAGATGTGCG AAGAAAAGAA 
AAGGAAAACA GAAAATTACA TATGGAGCTT AAGTCTGAAC GTGAAAAATT GACACAACAG 
ATGATCAAAT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGCCAA 
ATTCGAATAG AACTGCAGAT GACCCTGGAC AGTAAAGACA GTGACATTGA ACAGCTTCGG 
TCGCAGCTCC AGGCCTTGCA TATTGGTATG GACAGTTCCA GTATAGGCAG TGGACCAGGG 
GATGCTGAGC CTGATGACGG ATTTCCAGTC CATATCACTC AGTCACACAC TATGGAATCC 
ATGTCCTTCA CCTACCAGCG GTCCTCTACC TCTCTCAATA TTGCCACTAA GCCCTCCAGT 
TCTCACATGC TTCTTGACTC TGACTCTGAA GAAGATTCCT TGCCTTACTT ACCTAGTTCA 
TCGGAGCCTA TTAGTACAGA ATCACGATTA GAAGGATGGC TGTCGTTGCC TGTGCGGAAC 
AACACTAAAA AGTTTGGATG GGTTAAGAAG TATGTGATTG TAAGCAGCAA GAAGATTCTT 
TTCTATGATA GTGAACAAGA TAAAGAGCAG TCTAACCCTT ACATGGTGTT AGACATAGAC 
AAGCTATTTC ATGTCCGACC TGTTACTCAG ACAGATGTAT ACAGAGCAGA TGCTAAAGAA 
ATTCCAAGGA TATTTCAGAT CCTGTATGCC AATGAAGGAG AAAGTAAAAA GGAACCAGAA 
TTTCCAGTGG AACCAGTGGG AGAAAAATCT AATTATATTT GCCACAAAGG ACATGAGTTT 
ATTCCTACTC TTTATCACTT CCCAACCAAC TGTGAGGCCT GTATGAAGCC ACTGTGGCAC 
ATGTTTAAAC CTCCTCCTGC TCTAGAGTGC CGTAGATGCC ACATTAAATG TCATAAAGAT 
CACATGGACA AAAAGGAAGA GATCATAGCC CCCTGCAAAG TTTATTATGA TATATCATCG 
GCAAAGAATC TGTTGTTATT AGCAAATTCA ACGGAAGAAC AGCAGAAATG GGTTAGTCGG 
TTGGTGAAAA AGATACCTAA AAAGCCTCCA GCTCCAGACC CTTTTGCCCG ATCATCCCCT 
AGAACCTCAA TGAAAATACA ACAAAACCAG TCCATTCGGC GGCCCAGTCG ACAGCTTGCT 
CCAAACAAAC CAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR‑335 promotes stress granule formation to inhibit apoptosis by targeting ROCK2 in acute ischemic stroke.
International journal of molecular medicine43(3)1452-1466(2019 Mar)
Si W,Ye S,Ren Z,Liu X,Wu Z,Li Y,Zhou J,Zhang S,Li Y,Deng R,Chen D

ROCK2 regulates autophagy in the hippocampus of rats after subarachnoid hemorrhage.
Neuroreport29(18)1571-1577(2018 12)
Sun L,Ma Y,Zhang Z,Li X,Chen Y,Liu G,Fu A

RhoA/ROCK signaling regulates smooth muscle phenotypic modulation and vascular remodeling via the JNK pathway and vimentin cytoskeleton.
Pharmacological research133201-212(2018 07)
Tang L,Dai F,Liu Y,Yu X,Huang C,Wang Y,Yao W

Exercise increases Rho-kinase activity and insulin signaling in skeletal muscle.
Journal of cellular physiology233(6)4791-4800(2018 06)
Muñoz VR,Gaspar RC,Kuga GK,da Rocha AL,Crisol BM,Botezelli JD,Baptista IL,Mekary RA,da Silva ASR,Cintra DE,de Moura LP,Ropelle ER,Pauli JR

Neuroprotection Mediated by Upregulation of Endothelial Nitric Oxide Synthase in Rho-Associated, Coiled-Coil-Containing Kinase 2 Deficient Mice.
Circulation journal : official journal of the Japanese Circulation Society82(4)1195-1204(2018 03)
Hiroi Y,Noma K,Kim HH,Sladojevic N,Tabit CE,Li Y,Soydan G,Salomone S,Moskowitz MA,Liao JK

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Rock2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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