ROCK2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROCK2
Entrez Gene ID	374182
Full Name	Rho associated coiled-coil containing protein kinase 2
General protein information	Preferred Names Rho associated coiled-coil containing protein kinase 2 Names rho-associated protein kinase 2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015276087.1	XP_015131573.1	rho-associated protein kinase 2 isoform X3
XM_015276087.2	XP_015131573.1	rho-associated protein kinase 2 isoform X3
XM_015276085.1	XP_015131571.1	rho-associated protein kinase 2 isoform X1
XM_015276086.1	XP_015131572.1	rho-associated protein kinase 2 isoform X2
XM_015276086.2	XP_015131572.1	rho-associated protein kinase 2 isoform X2
XM_015276085.2	XP_015131571.1	rho-associated protein kinase 2 isoform X1
XM_025149412.1	XP_025005180.1	rho-associated protein kinase 2 isoform X4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rock2	NP_033098.2
Drosophila melanogaster (fruit fly)	rok	NP_536796.2
Caenorhabditis elegans (roundworm)	let-502	NP_491440.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000406	XP_310694.5
Pan troglodytes (chimpanzee)	ROCK2	XP_525689.3
Rattus norvegicus (Norway rat)	Rock2	NP_037154.2
Xenopus tropicalis (tropical clawed frog)	rock2	XP_004914525.1
Homo sapiens (human)	ROCK2	NP_004841.2
Macaca mulatta (Rhesus monkey)	ROCK2	XP_001096931.2
Canis lupus familiaris (dog)	ROCK2	XP_540083.4
Danio rerio (zebrafish)	rock2a	NP_777288.1
Bos taurus (cattle)	ROCK2	NP_776877.1
Gallus gallus (chicken)	ROCK2	XP_004935820.1

Gene Ontology

The following ROCK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROCK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa149176	XM_025149412.1 Latest version!	Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa34282	XM_015276085.2 Latest version!	Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa34283	XM_015276086.2 Latest version!	Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa34284	XM_015276087.2 Latest version!	Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OGa34284	XM_015276087.1	Gallus gallus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa34283	XM_015276086.1	Gallus gallus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OGa34282	XM_015276085.1	Gallus gallus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROCK2 ( XM_025149412.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025005180.1 Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa149176
Clone ID Related Accession (Same CDS sequence)	XM_025149412.1
Accession Version	XM_025149412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGAAT CAAGATTGGA AGGCTGGTTA 
TCACTACCTG TTCGAAATAA CACTAAAAAA TTTGGTTGGG TTAAAAAGTA TGTAATTGTA 
AGCAGTAAGA AGATCCTGTT CTATGACAGT GAACAAGATA AAGAACAATC TAATCCCTAT 
ATGGTATTAG ACATAGACAA ACTGTTCCAT GTCCGACCAG TCACCCAGAC TGATGTGTAC 
AGAGCAGATG CCAAGGAAAT TCCAAGGATA TTCCAGATTC TGTATGCTAA TGAAGGAGAG 
AGCAAAAAGG AGCAGGAATT TCCTGTGGAG CCTATGGGAG AGAAGTCAAA TTATATTTGT 
CACAAAGGAC ATGAGTTTAT ACCTACTCTT TATCACTTTC CAACCAATTG TGAAGCTTGT 
ATGAAGCCTC TTTGGCATAT GTTTAAACCT CCTCCTGCTC TAGAATGTCG CCGTTGCCAT 
ATCAAATGTC ACAAAGATCA TATGGATAAA AAAGAAGAGA TTATAGCACC TTGCAAAGTG 
TATTACGATA TTTCAACGGC AAAGAATCTA CTACTGTTAG CTAATTCTAC AGAAGAACAG 
CAAAAATGGG TGAGCCGACT GGTGAAAAAA ATACCCAAGA AACCTCCAGC ACCAGACCCC 
TTTGCTCGAT CTTCTCCCAG AACTTCTATG AAGGTACAGC CAAACCAGTC CATCAGACGA 
CCAAGTCGAC AGCTTCCTCC AAACAAACCA AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025005180.1
CDS	543..4718
Translation	MSQQLQAGKMSAAPLAPQAPDSAAAAGMLEGRQRKLESMIRDPRSPVNVESLLDGLNSLVLDLDYPALRKNKNIDNFLNRYEKIVEKIRALQMKAEDYDVVKVIGRGAFGEVQLVRHKMTQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLHFPDDVEISKHAKNLICAFLTDRDVRLGRNGVEEIKHHPFFKSDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRDNLLLSDSSQSCRENESVQSSKNEFQKKVSKLEEQLSNELQAKDELEQKYRSANTRLEKIVKELDEEITSRKNVESAVRQLEREKALLQHKNTEYQRKAEHEADKKRNLENEVNSLKDQLEDLKKRNQNSQISNEKINQLQRQLDEANSLLRSESETAARLRKNQTESTKQIQQLEANNRELQDKNCLLENAKLKLEKDFLNLQSALESERRDRSHGSEIISDLQGRISSLEEEVKNGKSALAKLEMEKRQLQEKLTDLEKEKSNMEIDMTYKFKVMQQNLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERALKQKVENRLLEAEKQRSMLDCDLKQSQQKINELLRQKDKLNEDVKNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLQEIKLSLEKQNNELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKEMQQKIQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELAEKDATIASLEEANRTLTSDVANLANEKEELNNKLKEEQEQITKLKEEEANIGNIKAQFEKQLLNERTLKTQAVNKLAEIMNRKGPVKRGADTDVRRKEKENRKLHMELKSEREKLTQMMIKYQKEINEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQSLHIGLDNSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPMGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKVQPNQSIRRPSRQLPPNKPS

Target ORF information:

RefSeq Version	XM_025149412.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149412.1

ORF Insert Sequence:

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGAAT CAAGATTGGA AGGCTGGTTA 
TCACTACCTG TTCGAAATAA CACTAAAAAA TTTGGTTGGG TTAAAAAGTA TGTAATTGTA 
AGCAGTAAGA AGATCCTGTT CTATGACAGT GAACAAGATA AAGAACAATC TAATCCCTAT 
ATGGTATTAG ACATAGACAA ACTGTTCCAT GTCCGACCAG TCACCCAGAC TGATGTGTAC 
AGAGCAGATG CCAAGGAAAT TCCAAGGATA TTCCAGATTC TGTATGCTAA TGAAGGAGAG 
AGCAAAAAGG AGCAGGAATT TCCTGTGGAG CCTATGGGAG AGAAGTCAAA TTATATTTGT 
CACAAAGGAC ATGAGTTTAT ACCTACTCTT TATCACTTTC CAACCAATTG TGAAGCTTGT 
ATGAAGCCTC TTTGGCATAT GTTTAAACCT CCTCCTGCTC TAGAATGTCG CCGTTGCCAT 
ATCAAATGTC ACAAAGATCA TATGGATAAA AAAGAAGAGA TTATAGCACC TTGCAAAGTG 
TATTACGATA TTTCAACGGC AAAGAATCTA CTACTGTTAG CTAATTCTAC AGAAGAACAG 
CAAAAATGGG TGAGCCGACT GGTGAAAAAA ATACCCAAGA AACCTCCAGC ACCAGACCCC 
TTTGCTCGAT CTTCTCCCAG AACTTCTATG AAGGTACAGC CAAACCAGTC CATCAGACGA 
CCAAGTCGAC AGCTTCCTCC AAACAAACCA AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_015276085.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131571.1 Gallus gallus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa34282
Clone ID Related Accession (Same CDS sequence)	XM_015276085.1 , XM_015276085.2
Accession Version	XM_015276085.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471673.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGTCC ATATCACTCA ATCCCACACT 
ATGGAATCCA TGTCTTTTAC CTACAAGCAC TCTTCCACCT CTGTCAGTAT TGCCACTAAG 
CCTTCCAGTT CTCGCATGCT TCTCGATTCT GATTCTGACT CAGAAGAAGA ACCTTTGTCT 
TGTTCTCACA GCCCTACAGA AGTTGATAGC ACAGAATCAA GATTGGAAGG CTGGTTATCA 
CTACCTGTTC GAAATAACAC TAAAAAATTT GGTTGGGTTA AAAAGTATGT AATTGTAAGC 
AGTAAGAAGA TCCTGTTCTA TGACAGTGAA CAAGATAAAG AACAATCTAA TCCCTATATG 
GTATTAGACA TAGACAAACT GTTCCATGTC CGACCAGTCA CCCAGACTGA TGTGTACAGA 
GCAGATGCCA AGGAAATTCC AAGGATATTC CAGATTCTGT ATGCTAATGA AGGAGAGAGC 
AAAAAGGAGC AGGAATTTCC TGTGGAGCCT ATGGGAGAGA AGTCAAATTA TATTTGTCAC 
AAAGGACATG AGTTTATACC TACTCTTTAT CACTTTCCAA CCAATTGTGA AGCTTGTATG 
AAGCCTCTTT GGCATATGTT TAAACCTCCT CCTGCTCTAG AATGTCGCCG TTGCCATATC 
AAATGTCACA AAGATCATAT GGATAAAAAA GAAGAGATTA TAGCACCTTG CAAAGTGTAT 
TACGATATTT CAACGGCAAA GAATCTACTA CTGTTAGCTA ATTCTACAGA AGAACAGCAA 
AAATGGGTGA GCCGACTGGT GAAAAAAATA CCCAAGAAAC CTCCAGCACC AGACCCCTTT 
GCTCGATCTT CTCCCAGAAC TTCTATGAAG GTACAGCCAA ACCAGTCCAT CAGACGACCA 
AGTCGACAGC TTCCTCCAAA CAAACCAAGA TTGCTTGATC TGGCATTTAA AGACTGGGAT 
TGGTCATTTG ATGATGTTGA TGATATTGAT GATGATGATG ATGATGATGA TGACGATGTT 
TTTGATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131571.1
CDS	21..4472
Translation	MSQQLQAGKMSAAPLAPQAPDSAAAAGMLEGRQRKLESMIRDPRSPVNVESLLDGLNSLVLDLDYPALRKNKNIDNFLNRYEKIVEKIRALQMKAEDYDVVKVIGRGAFGEVQLVRHKMTQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLHFPDDVEISKHAKNLICAFLTDRDVRLGRNGVEEIKHHPFFKSDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRDNLLLSDSSQSCRENESVQSSKNEFQKKVSKLEEQLSNELQAKDELEQKYRSANTRLEKIVKELDEEITSRKNVESAVRQLEREKALLQHKNTEYQRKAEHEADKKRNLENEVNSLKDQLEDLKKRNQNSQISNEKINQLQRQLDEANSLLRSESETAARLRKNQTESTKQIQQLEANNRELQDKNCLLENAKLKLEKDFLNLQSALESERRDRSHGSEIISDLQGRISSLEEEVKNGKSALAKLEMEKRQLQEKLTDLEKEKSNMEIDMTYKFKVMQQNLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERALKQKVENRLLEAEKQRSMLDCDLKQSQQKINELLRQKDKLNEDVKNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLQEIKLSLEKQNNELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKEMQQKIQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELAEKDATIASLEEANRTLTSDVANLANEKEELNNKLKEEQEQITKLKEEEANIGNIKAQFEKQLLNERTLKTQAVNKLAEIMNRKGPVKRGADTDVRRKEKENRKLHMELKSEREKLTQMMIKYQKEINEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQSLHIGLDNSSIGSGPGDAEADDGFPVHITQSHTMESMSFTYKHSSTSVSIATKPSSSRMLLDSDSDSEEEPLSCSHSPTEVDSTESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPMGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKVQPNQSIRRPSRQLPPNKPRLLDLAFKDWDWSFDDVDDIDDDDDDDDDDVFDF

Target ORF information:

RefSeq Version	XM_015276085.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276085.1

ORF Insert Sequence:

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGTCC ATATCACTCA ATCCCACACT 
ATGGAATCCA TGTCTTTTAC CTACAAGCAC TCTTCCACCT CTGTCAGTAT TGCCACTAAG 
CCTTCCAGTT CTCGCATGCT TCTCGATTCT GATTCTGACT CAGAAGAAGA ACCTTTGTCT 
TGTTCTCACA GCCCTACAGA AGTTGATAGC ACAGAATCAA GATTGGAAGG CTGGTTATCA 
CTACCTGTTC GAAATAACAC TAAAAAATTT GGTTGGGTTA AAAAGTATGT AATTGTAAGC 
AGTAAGAAGA TCCTGTTCTA TGACAGTGAA CAAGATAAAG AACAATCTAA TCCCTATATG 
GTATTAGACA TAGACAAACT GTTCCATGTC CGACCAGTCA CCCAGACTGA TGTGTACAGA 
GCAGATGCCA AGGAAATTCC AAGGATATTC CAGATTCTGT ATGCTAATGA AGGAGAGAGC 
AAAAAGGAGC AGGAATTTCC TGTGGAGCCT ATGGGAGAGA AGTCAAATTA TATTTGTCAC 
AAAGGACATG AGTTTATACC TACTCTTTAT CACTTTCCAA CCAATTGTGA AGCTTGTATG 
AAGCCTCTTT GGCATATGTT TAAACCTCCT CCTGCTCTAG AATGTCGCCG TTGCCATATC 
AAATGTCACA AAGATCATAT GGATAAAAAA GAAGAGATTA TAGCACCTTG CAAAGTGTAT 
TACGATATTT CAACGGCAAA GAATCTACTA CTGTTAGCTA ATTCTACAGA AGAACAGCAA 
AAATGGGTGA GCCGACTGGT GAAAAAAATA CCCAAGAAAC CTCCAGCACC AGACCCCTTT 
GCTCGATCTT CTCCCAGAAC TTCTATGAAG GTACAGCCAA ACCAGTCCAT CAGACGACCA 
AGTCGACAGC TTCCTCCAAA CAAACCAAGA TTGCTTGATC TGGCATTTAA AGACTGGGAT 
TGGTCATTTG ATGATGTTGA TGATATTGAT GATGATGATG ATGATGATGA TGACGATGTT 
TTTGATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_015276085.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131571.1 Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

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CloneID	OGa34282
Clone ID Related Accession (Same CDS sequence)	XM_015276085.1 , XM_015276085.2
Accession Version	XM_015276085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGTCC ATATCACTCA ATCCCACACT 
ATGGAATCCA TGTCTTTTAC CTACAAGCAC TCTTCCACCT CTGTCAGTAT TGCCACTAAG 
CCTTCCAGTT CTCGCATGCT TCTCGATTCT GATTCTGACT CAGAAGAAGA ACCTTTGTCT 
TGTTCTCACA GCCCTACAGA AGTTGATAGC ACAGAATCAA GATTGGAAGG CTGGTTATCA 
CTACCTGTTC GAAATAACAC TAAAAAATTT GGTTGGGTTA AAAAGTATGT AATTGTAAGC 
AGTAAGAAGA TCCTGTTCTA TGACAGTGAA CAAGATAAAG AACAATCTAA TCCCTATATG 
GTATTAGACA TAGACAAACT GTTCCATGTC CGACCAGTCA CCCAGACTGA TGTGTACAGA 
GCAGATGCCA AGGAAATTCC AAGGATATTC CAGATTCTGT ATGCTAATGA AGGAGAGAGC 
AAAAAGGAGC AGGAATTTCC TGTGGAGCCT ATGGGAGAGA AGTCAAATTA TATTTGTCAC 
AAAGGACATG AGTTTATACC TACTCTTTAT CACTTTCCAA CCAATTGTGA AGCTTGTATG 
AAGCCTCTTT GGCATATGTT TAAACCTCCT CCTGCTCTAG AATGTCGCCG TTGCCATATC 
AAATGTCACA AAGATCATAT GGATAAAAAA GAAGAGATTA TAGCACCTTG CAAAGTGTAT 
TACGATATTT CAACGGCAAA GAATCTACTA CTGTTAGCTA ATTCTACAGA AGAACAGCAA 
AAATGGGTGA GCCGACTGGT GAAAAAAATA CCCAAGAAAC CTCCAGCACC AGACCCCTTT 
GCTCGATCTT CTCCCAGAAC TTCTATGAAG GTACAGCCAA ACCAGTCCAT CAGACGACCA 
AGTCGACAGC TTCCTCCAAA CAAACCAAGA TTGCTTGATC TGGCATTTAA AGACTGGGAT 
TGGTCATTTG ATGATGTTGA TGATATTGAT GATGATGATG ATGATGATGA TGACGATGTT 
TTTGATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131571.1
CDS	544..4995
Translation	MSQQLQAGKMSAAPLAPQAPDSAAAAGMLEGRQRKLESMIRDPRSPVNVESLLDGLNSLVLDLDYPALRKNKNIDNFLNRYEKIVEKIRALQMKAEDYDVVKVIGRGAFGEVQLVRHKMTQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLHFPDDVEISKHAKNLICAFLTDRDVRLGRNGVEEIKHHPFFKSDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRDNLLLSDSSQSCRENESVQSSKNEFQKKVSKLEEQLSNELQAKDELEQKYRSANTRLEKIVKELDEEITSRKNVESAVRQLEREKALLQHKNTEYQRKAEHEADKKRNLENEVNSLKDQLEDLKKRNQNSQISNEKINQLQRQLDEANSLLRSESETAARLRKNQTESTKQIQQLEANNRELQDKNCLLENAKLKLEKDFLNLQSALESERRDRSHGSEIISDLQGRISSLEEEVKNGKSALAKLEMEKRQLQEKLTDLEKEKSNMEIDMTYKFKVMQQNLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERALKQKVENRLLEAEKQRSMLDCDLKQSQQKINELLRQKDKLNEDVKNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLQEIKLSLEKQNNELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKEMQQKIQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELAEKDATIASLEEANRTLTSDVANLANEKEELNNKLKEEQEQITKLKEEEANIGNIKAQFEKQLLNERTLKTQAVNKLAEIMNRKGPVKRGADTDVRRKEKENRKLHMELKSEREKLTQMMIKYQKEINEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQSLHIGLDNSSIGSGPGDAEADDGFPVHITQSHTMESMSFTYKHSSTSVSIATKPSSSRMLLDSDSDSEEEPLSCSHSPTEVDSTESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPMGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKVQPNQSIRRPSRQLPPNKPRLLDLAFKDWDWSFDDVDDIDDDDDDDDDDVFDF

Target ORF information:

RefSeq Version	XM_015276085.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276085.2

ORF Insert Sequence:

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGTCC ATATCACTCA ATCCCACACT 
ATGGAATCCA TGTCTTTTAC CTACAAGCAC TCTTCCACCT CTGTCAGTAT TGCCACTAAG 
CCTTCCAGTT CTCGCATGCT TCTCGATTCT GATTCTGACT CAGAAGAAGA ACCTTTGTCT 
TGTTCTCACA GCCCTACAGA AGTTGATAGC ACAGAATCAA GATTGGAAGG CTGGTTATCA 
CTACCTGTTC GAAATAACAC TAAAAAATTT GGTTGGGTTA AAAAGTATGT AATTGTAAGC 
AGTAAGAAGA TCCTGTTCTA TGACAGTGAA CAAGATAAAG AACAATCTAA TCCCTATATG 
GTATTAGACA TAGACAAACT GTTCCATGTC CGACCAGTCA CCCAGACTGA TGTGTACAGA 
GCAGATGCCA AGGAAATTCC AAGGATATTC CAGATTCTGT ATGCTAATGA AGGAGAGAGC 
AAAAAGGAGC AGGAATTTCC TGTGGAGCCT ATGGGAGAGA AGTCAAATTA TATTTGTCAC 
AAAGGACATG AGTTTATACC TACTCTTTAT CACTTTCCAA CCAATTGTGA AGCTTGTATG 
AAGCCTCTTT GGCATATGTT TAAACCTCCT CCTGCTCTAG AATGTCGCCG TTGCCATATC 
AAATGTCACA AAGATCATAT GGATAAAAAA GAAGAGATTA TAGCACCTTG CAAAGTGTAT 
TACGATATTT CAACGGCAAA GAATCTACTA CTGTTAGCTA ATTCTACAGA AGAACAGCAA 
AAATGGGTGA GCCGACTGGT GAAAAAAATA CCCAAGAAAC CTCCAGCACC AGACCCCTTT 
GCTCGATCTT CTCCCAGAAC TTCTATGAAG GTACAGCCAA ACCAGTCCAT CAGACGACCA 
AGTCGACAGC TTCCTCCAAA CAAACCAAGA TTGCTTGATC TGGCATTTAA AGACTGGGAT 
TGGTCATTTG ATGATGTTGA TGATATTGAT GATGATGATG ATGATGATGA TGACGATGTT 
TTTGATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_015276086.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131572.1 Gallus gallus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

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CloneID	OGa34283
Clone ID Related Accession (Same CDS sequence)	XM_015276086.1 , XM_015276086.2
Accession Version	XM_015276086.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471673.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGTCC ATATCACTCA ATCCCACACT 
ATGGAATCCA TGTCTTTTAC CTACAAGCAC TCTTCCACCT CTGTCAGTAT TGCCACTAAG 
CCTTCCAGTT CTCGCATGCT TCTCGATTCT GATTCTGACT CAGAAGAAGA ACCTTTGTCT 
TGTTCTCACA GCCCTACAGA AGTTGATAGC ACAGAATCAA GATTGGAAGG CTGGTTATCA 
CTACCTGTTC GAAATAACAC TAAAAAATTT GGTTGGGTTA AAAAGTATGT AATTGTAAGC 
AGTAAGAAGA TCCTGTTCTA TGACAGTGAA CAAGATAAAG AACAATCTAA TCCCTATATG 
GTATTAGACA TAGACAAACT GTTCCATGTC CGACCAGTCA CCCAGACTGA TGTGTACAGA 
GCAGATGCCA AGGAAATTCC AAGGATATTC CAGATTCTGT ATGCTAATGA AGGAGAGAGC 
AAAAAGGAGC AGGAATTTCC TGTGGAGCCT ATGGGAGAGA AGTCAAATTA TATTTGTCAC 
AAAGGACATG AGTTTATACC TACTCTTTAT CACTTTCCAA CCAATTGTGA AGCTTGTATG 
AAGCCTCTTT GGCATATGTT TAAACCTCCT CCTGCTCTAG AATGTCGCCG TTGCCATATC 
AAATGTCACA AAGATCATAT GGATAAAAAA GAAGAGATTA TAGCACCTTG CAAAGTGTAT 
TACGATATTT CAACGGCAAA GAATCTACTA CTGTTAGCTA ATTCTACAGA AGAACAGCAA 
AAATGGGTGA GCCGACTGGT GAAAAAAATA CCCAAGAAAC CTCCAGCACC AGACCCCTTT 
GCTCGATCTT CTCCCAGAAC TTCTATGAAG GTACAGCCAA ACCAGTCCAT CAGACGACCA 
AGTCGACAGC TTCCTCCAAA CAAACCAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131572.1
CDS	21..4373
Translation	MSQQLQAGKMSAAPLAPQAPDSAAAAGMLEGRQRKLESMIRDPRSPVNVESLLDGLNSLVLDLDYPALRKNKNIDNFLNRYEKIVEKIRALQMKAEDYDVVKVIGRGAFGEVQLVRHKMTQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLHFPDDVEISKHAKNLICAFLTDRDVRLGRNGVEEIKHHPFFKSDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRDNLLLSDSSQSCRENESVQSSKNEFQKKVSKLEEQLSNELQAKDELEQKYRSANTRLEKIVKELDEEITSRKNVESAVRQLEREKALLQHKNTEYQRKAEHEADKKRNLENEVNSLKDQLEDLKKRNQNSQISNEKINQLQRQLDEANSLLRSESETAARLRKNQTESTKQIQQLEANNRELQDKNCLLENAKLKLEKDFLNLQSALESERRDRSHGSEIISDLQGRISSLEEEVKNGKSALAKLEMEKRQLQEKLTDLEKEKSNMEIDMTYKFKVMQQNLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERALKQKVENRLLEAEKQRSMLDCDLKQSQQKINELLRQKDKLNEDVKNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLQEIKLSLEKQNNELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKEMQQKIQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELAEKDATIASLEEANRTLTSDVANLANEKEELNNKLKEEQEQITKLKEEEANIGNIKAQFEKQLLNERTLKTQAVNKLAEIMNRKGPVKRGADTDVRRKEKENRKLHMELKSEREKLTQMMIKYQKEINEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQSLHIGLDNSSIGSGPGDAEADDGFPVHITQSHTMESMSFTYKHSSTSVSIATKPSSSRMLLDSDSDSEEEPLSCSHSPTEVDSTESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPMGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKVQPNQSIRRPSRQLPPNKPS

Target ORF information:

RefSeq Version	XM_015276086.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276086.1

ORF Insert Sequence:

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGTCC ATATCACTCA ATCCCACACT 
ATGGAATCCA TGTCTTTTAC CTACAAGCAC TCTTCCACCT CTGTCAGTAT TGCCACTAAG 
CCTTCCAGTT CTCGCATGCT TCTCGATTCT GATTCTGACT CAGAAGAAGA ACCTTTGTCT 
TGTTCTCACA GCCCTACAGA AGTTGATAGC ACAGAATCAA GATTGGAAGG CTGGTTATCA 
CTACCTGTTC GAAATAACAC TAAAAAATTT GGTTGGGTTA AAAAGTATGT AATTGTAAGC 
AGTAAGAAGA TCCTGTTCTA TGACAGTGAA CAAGATAAAG AACAATCTAA TCCCTATATG 
GTATTAGACA TAGACAAACT GTTCCATGTC CGACCAGTCA CCCAGACTGA TGTGTACAGA 
GCAGATGCCA AGGAAATTCC AAGGATATTC CAGATTCTGT ATGCTAATGA AGGAGAGAGC 
AAAAAGGAGC AGGAATTTCC TGTGGAGCCT ATGGGAGAGA AGTCAAATTA TATTTGTCAC 
AAAGGACATG AGTTTATACC TACTCTTTAT CACTTTCCAA CCAATTGTGA AGCTTGTATG 
AAGCCTCTTT GGCATATGTT TAAACCTCCT CCTGCTCTAG AATGTCGCCG TTGCCATATC 
AAATGTCACA AAGATCATAT GGATAAAAAA GAAGAGATTA TAGCACCTTG CAAAGTGTAT 
TACGATATTT CAACGGCAAA GAATCTACTA CTGTTAGCTA ATTCTACAGA AGAACAGCAA 
AAATGGGTGA GCCGACTGGT GAAAAAAATA CCCAAGAAAC CTCCAGCACC AGACCCCTTT 
GCTCGATCTT CTCCCAGAAC TTCTATGAAG GTACAGCCAA ACCAGTCCAT CAGACGACCA 
AGTCGACAGC TTCCTCCAAA CAAACCAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_015276086.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131572.1 Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

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CloneID	OGa34283
Clone ID Related Accession (Same CDS sequence)	XM_015276086.1 , XM_015276086.2
Accession Version	XM_015276086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGTCC ATATCACTCA ATCCCACACT 
ATGGAATCCA TGTCTTTTAC CTACAAGCAC TCTTCCACCT CTGTCAGTAT TGCCACTAAG 
CCTTCCAGTT CTCGCATGCT TCTCGATTCT GATTCTGACT CAGAAGAAGA ACCTTTGTCT 
TGTTCTCACA GCCCTACAGA AGTTGATAGC ACAGAATCAA GATTGGAAGG CTGGTTATCA 
CTACCTGTTC GAAATAACAC TAAAAAATTT GGTTGGGTTA AAAAGTATGT AATTGTAAGC 
AGTAAGAAGA TCCTGTTCTA TGACAGTGAA CAAGATAAAG AACAATCTAA TCCCTATATG 
GTATTAGACA TAGACAAACT GTTCCATGTC CGACCAGTCA CCCAGACTGA TGTGTACAGA 
GCAGATGCCA AGGAAATTCC AAGGATATTC CAGATTCTGT ATGCTAATGA AGGAGAGAGC 
AAAAAGGAGC AGGAATTTCC TGTGGAGCCT ATGGGAGAGA AGTCAAATTA TATTTGTCAC 
AAAGGACATG AGTTTATACC TACTCTTTAT CACTTTCCAA CCAATTGTGA AGCTTGTATG 
AAGCCTCTTT GGCATATGTT TAAACCTCCT CCTGCTCTAG AATGTCGCCG TTGCCATATC 
AAATGTCACA AAGATCATAT GGATAAAAAA GAAGAGATTA TAGCACCTTG CAAAGTGTAT 
TACGATATTT CAACGGCAAA GAATCTACTA CTGTTAGCTA ATTCTACAGA AGAACAGCAA 
AAATGGGTGA GCCGACTGGT GAAAAAAATA CCCAAGAAAC CTCCAGCACC AGACCCCTTT 
GCTCGATCTT CTCCCAGAAC TTCTATGAAG GTACAGCCAA ACCAGTCCAT CAGACGACCA 
AGTCGACAGC TTCCTCCAAA CAAACCAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131572.1
CDS	544..4896
Translation	MSQQLQAGKMSAAPLAPQAPDSAAAAGMLEGRQRKLESMIRDPRSPVNVESLLDGLNSLVLDLDYPALRKNKNIDNFLNRYEKIVEKIRALQMKAEDYDVVKVIGRGAFGEVQLVRHKMTQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLHFPDDVEISKHAKNLICAFLTDRDVRLGRNGVEEIKHHPFFKSDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRDNLLLSDSSQSCRENESVQSSKNEFQKKVSKLEEQLSNELQAKDELEQKYRSANTRLEKIVKELDEEITSRKNVESAVRQLEREKALLQHKNTEYQRKAEHEADKKRNLENEVNSLKDQLEDLKKRNQNSQISNEKINQLQRQLDEANSLLRSESETAARLRKNQTESTKQIQQLEANNRELQDKNCLLENAKLKLEKDFLNLQSALESERRDRSHGSEIISDLQGRISSLEEEVKNGKSALAKLEMEKRQLQEKLTDLEKEKSNMEIDMTYKFKVMQQNLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERALKQKVENRLLEAEKQRSMLDCDLKQSQQKINELLRQKDKLNEDVKNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLQEIKLSLEKQNNELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKEMQQKIQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELAEKDATIASLEEANRTLTSDVANLANEKEELNNKLKEEQEQITKLKEEEANIGNIKAQFEKQLLNERTLKTQAVNKLAEIMNRKGPVKRGADTDVRRKEKENRKLHMELKSEREKLTQMMIKYQKEINEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQSLHIGLDNSSIGSGPGDAEADDGFPVHITQSHTMESMSFTYKHSSTSVSIATKPSSSRMLLDSDSDSEEEPLSCSHSPTEVDSTESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPMGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKVQPNQSIRRPSRQLPPNKPS

Target ORF information:

RefSeq Version	XM_015276086.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276086.2

ORF Insert Sequence:

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGTCC ATATCACTCA ATCCCACACT 
ATGGAATCCA TGTCTTTTAC CTACAAGCAC TCTTCCACCT CTGTCAGTAT TGCCACTAAG 
CCTTCCAGTT CTCGCATGCT TCTCGATTCT GATTCTGACT CAGAAGAAGA ACCTTTGTCT 
TGTTCTCACA GCCCTACAGA AGTTGATAGC ACAGAATCAA GATTGGAAGG CTGGTTATCA 
CTACCTGTTC GAAATAACAC TAAAAAATTT GGTTGGGTTA AAAAGTATGT AATTGTAAGC 
AGTAAGAAGA TCCTGTTCTA TGACAGTGAA CAAGATAAAG AACAATCTAA TCCCTATATG 
GTATTAGACA TAGACAAACT GTTCCATGTC CGACCAGTCA CCCAGACTGA TGTGTACAGA 
GCAGATGCCA AGGAAATTCC AAGGATATTC CAGATTCTGT ATGCTAATGA AGGAGAGAGC 
AAAAAGGAGC AGGAATTTCC TGTGGAGCCT ATGGGAGAGA AGTCAAATTA TATTTGTCAC 
AAAGGACATG AGTTTATACC TACTCTTTAT CACTTTCCAA CCAATTGTGA AGCTTGTATG 
AAGCCTCTTT GGCATATGTT TAAACCTCCT CCTGCTCTAG AATGTCGCCG TTGCCATATC 
AAATGTCACA AAGATCATAT GGATAAAAAA GAAGAGATTA TAGCACCTTG CAAAGTGTAT 
TACGATATTT CAACGGCAAA GAATCTACTA CTGTTAGCTA ATTCTACAGA AGAACAGCAA 
AAATGGGTGA GCCGACTGGT GAAAAAAATA CCCAAGAAAC CTCCAGCACC AGACCCCTTT 
GCTCGATCTT CTCCCAGAAC TTCTATGAAG GTACAGCCAA ACCAGTCCAT CAGACGACCA 
AGTCGACAGC TTCCTCCAAA CAAACCAAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_015276087.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131573.1 Gallus gallus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa34284
Clone ID Related Accession (Same CDS sequence)	XM_015276087.1 , XM_015276087.2
Accession Version	XM_015276087.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1451750400000
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471673.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGAAT CAAGATTGGA AGGCTGGTTA 
TCACTACCTG TTCGAAATAA CACTAAAAAA TTTGGTTGGG TTAAAAAGTA TGTAATTGTA 
AGCAGTAAGA AGATCCTGTT CTATGACAGT GAACAAGATA AAGAACAATC TAATCCCTAT 
ATGGTATTAG ACATAGACAA ACTGTTCCAT GTCCGACCAG TCACCCAGAC TGATGTGTAC 
AGAGCAGATG CCAAGGAAAT TCCAAGGATA TTCCAGATTC TGTATGCTAA TGAAGGAGAG 
AGCAAAAAGG AGCAGGAATT TCCTGTGGAG CCTATGGGAG AGAAGTCAAA TTATATTTGT 
CACAAAGGAC ATGAGTTTAT ACCTACTCTT TATCACTTTC CAACCAATTG TGAAGCTTGT 
ATGAAGCCTC TTTGGCATAT GTTTAAACCT CCTCCTGCTC TAGAATGTCG CCGTTGCCAT 
ATCAAATGTC ACAAAGATCA TATGGATAAA AAAGAAGAGA TTATAGCACC TTGCAAAGTG 
TATTACGATA TTTCAACGGC AAAGAATCTA CTACTGTTAG CTAATTCTAC AGAAGAACAG 
CAAAAATGGG TGAGCCGACT GGTGAAAAAA ATACCCAAGA AACCTCCAGC ACCAGACCCC 
TTTGCTCGAT CTTCTCCCAG AACTTCTATG AAGGTACAGC CAAACCAGTC CATCAGACGA 
CCAAGTCGAC AGCTTCCTCC AAACAAACCA AGATTGCTTG ATCTGGCATT TAAAGACTGG 
GATTGGTCAT TTGATGATGT TGATGATATT GATGATGATG ATGATGATGA TGATGACGAT 
GTTTTTGATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131573.1
CDS	21..4295
Translation	MSQQLQAGKMSAAPLAPQAPDSAAAAGMLEGRQRKLESMIRDPRSPVNVESLLDGLNSLVLDLDYPALRKNKNIDNFLNRYEKIVEKIRALQMKAEDYDVVKVIGRGAFGEVQLVRHKMTQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLHFPDDVEISKHAKNLICAFLTDRDVRLGRNGVEEIKHHPFFKSDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRDNLLLSDSSQSCRENESVQSSKNEFQKKVSKLEEQLSNELQAKDELEQKYRSANTRLEKIVKELDEEITSRKNVESAVRQLEREKALLQHKNTEYQRKAEHEADKKRNLENEVNSLKDQLEDLKKRNQNSQISNEKINQLQRQLDEANSLLRSESETAARLRKNQTESTKQIQQLEANNRELQDKNCLLENAKLKLEKDFLNLQSALESERRDRSHGSEIISDLQGRISSLEEEVKNGKSALAKLEMEKRQLQEKLTDLEKEKSNMEIDMTYKFKVMQQNLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERALKQKVENRLLEAEKQRSMLDCDLKQSQQKINELLRQKDKLNEDVKNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLQEIKLSLEKQNNELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKEMQQKIQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELAEKDATIASLEEANRTLTSDVANLANEKEELNNKLKEEQEQITKLKEEEANIGNIKAQFEKQLLNERTLKTQAVNKLAEIMNRKGPVKRGADTDVRRKEKENRKLHMELKSEREKLTQMMIKYQKEINEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQSLHIGLDNSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPMGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKVQPNQSIRRPSRQLPPNKPRLLDLAFKDWDWSFDDVDDIDDDDDDDDDDVFDF

Target ORF information:

RefSeq Version	XM_015276087.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276087.1

ORF Insert Sequence:

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGAAT CAAGATTGGA AGGCTGGTTA 
TCACTACCTG TTCGAAATAA CACTAAAAAA TTTGGTTGGG TTAAAAAGTA TGTAATTGTA 
AGCAGTAAGA AGATCCTGTT CTATGACAGT GAACAAGATA AAGAACAATC TAATCCCTAT 
ATGGTATTAG ACATAGACAA ACTGTTCCAT GTCCGACCAG TCACCCAGAC TGATGTGTAC 
AGAGCAGATG CCAAGGAAAT TCCAAGGATA TTCCAGATTC TGTATGCTAA TGAAGGAGAG 
AGCAAAAAGG AGCAGGAATT TCCTGTGGAG CCTATGGGAG AGAAGTCAAA TTATATTTGT 
CACAAAGGAC ATGAGTTTAT ACCTACTCTT TATCACTTTC CAACCAATTG TGAAGCTTGT 
ATGAAGCCTC TTTGGCATAT GTTTAAACCT CCTCCTGCTC TAGAATGTCG CCGTTGCCAT 
ATCAAATGTC ACAAAGATCA TATGGATAAA AAAGAAGAGA TTATAGCACC TTGCAAAGTG 
TATTACGATA TTTCAACGGC AAAGAATCTA CTACTGTTAG CTAATTCTAC AGAAGAACAG 
CAAAAATGGG TGAGCCGACT GGTGAAAAAA ATACCCAAGA AACCTCCAGC ACCAGACCCC 
TTTGCTCGAT CTTCTCCCAG AACTTCTATG AAGGTACAGC CAAACCAGTC CATCAGACGA 
CCAAGTCGAC AGCTTCCTCC AAACAAACCA AGATTGCTTG ATCTGGCATT TAAAGACTGG 
GATTGGTCAT TTGATGATGT TGATGATATT GATGATGATG ATGATGATGA TGATGACGAT 
GTTTTTGATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_015276087.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015131573.1 Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.

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CloneID	OGa34284
Clone ID Related Accession (Same CDS sequence)	XM_015276087.1 , XM_015276087.2
Accession Version	XM_015276087.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1526400000000
Organism	Gallus gallus(chicken)
Product	rho-associated protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015276087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGAAT CAAGATTGGA AGGCTGGTTA 
TCACTACCTG TTCGAAATAA CACTAAAAAA TTTGGTTGGG TTAAAAAGTA TGTAATTGTA 
AGCAGTAAGA AGATCCTGTT CTATGACAGT GAACAAGATA AAGAACAATC TAATCCCTAT 
ATGGTATTAG ACATAGACAA ACTGTTCCAT GTCCGACCAG TCACCCAGAC TGATGTGTAC 
AGAGCAGATG CCAAGGAAAT TCCAAGGATA TTCCAGATTC TGTATGCTAA TGAAGGAGAG 
AGCAAAAAGG AGCAGGAATT TCCTGTGGAG CCTATGGGAG AGAAGTCAAA TTATATTTGT 
CACAAAGGAC ATGAGTTTAT ACCTACTCTT TATCACTTTC CAACCAATTG TGAAGCTTGT 
ATGAAGCCTC TTTGGCATAT GTTTAAACCT CCTCCTGCTC TAGAATGTCG CCGTTGCCAT 
ATCAAATGTC ACAAAGATCA TATGGATAAA AAAGAAGAGA TTATAGCACC TTGCAAAGTG 
TATTACGATA TTTCAACGGC AAAGAATCTA CTACTGTTAG CTAATTCTAC AGAAGAACAG 
CAAAAATGGG TGAGCCGACT GGTGAAAAAA ATACCCAAGA AACCTCCAGC ACCAGACCCC 
TTTGCTCGAT CTTCTCCCAG AACTTCTATG AAGGTACAGC CAAACCAGTC CATCAGACGA 
CCAAGTCGAC AGCTTCCTCC AAACAAACCA AGATTGCTTG ATCTGGCATT TAAAGACTGG 
GATTGGTCAT TTGATGATGT TGATGATATT GATGATGATG ATGATGATGA TGATGACGAT 
GTTTTTGATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015131573.1
CDS	543..4817
Translation	MSQQLQAGKMSAAPLAPQAPDSAAAAGMLEGRQRKLESMIRDPRSPVNVESLLDGLNSLVLDLDYPALRKNKNIDNFLNRYEKIVEKIRALQMKAEDYDVVKVIGRGAFGEVQLVRHKMTQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLHFPDDVEISKHAKNLICAFLTDRDVRLGRNGVEEIKHHPFFKSDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRDNLLLSDSSQSCRENESVQSSKNEFQKKVSKLEEQLSNELQAKDELEQKYRSANTRLEKIVKELDEEITSRKNVESAVRQLEREKALLQHKNTEYQRKAEHEADKKRNLENEVNSLKDQLEDLKKRNQNSQISNEKINQLQRQLDEANSLLRSESETAARLRKNQTESTKQIQQLEANNRELQDKNCLLENAKLKLEKDFLNLQSALESERRDRSHGSEIISDLQGRISSLEEEVKNGKSALAKLEMEKRQLQEKLTDLEKEKSNMEIDMTYKFKVMQQNLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERALKQKVENRLLEAEKQRSMLDCDLKQSQQKINELLRQKDKLNEDVKNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLQEIKLSLEKQNNELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKEMQQKIQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELAEKDATIASLEEANRTLTSDVANLANEKEELNNKLKEEQEQITKLKEEEANIGNIKAQFEKQLLNERTLKTQAVNKLAEIMNRKGPVKRGADTDVRRKEKENRKLHMELKSEREKLTQMMIKYQKEINEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQSLHIGLDNSSIGSGPGDAEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPMGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISTAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKVQPNQSIRRPSRQLPPNKPRLLDLAFKDWDWSFDDVDDIDDDDDDDDDDVFDF

Target ORF information:

RefSeq Version	XM_015276087.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015276087.2

ORF Insert Sequence:

ATGAGCCAGC AGCTGCAGGC GGGGAAGATG TCGGCGGCTC CCCTCGCGCC GCAGGCTCCG 
GACTCGGCGG CGGCGGCGGG GATGCTCGAG GGCCGCCAGA GAAAGCTGGA GTCTATGATC 
CGCGACCCGC GCTCTCCGGT CAACGTGGAG AGCCTACTGG ATGGCTTGAA TTCTTTGGTC 
CTTGATCTGG ATTATCCGGC GCTGAGGAAG AACAAGAACA TTGATAATTT CCTAAATAGA 
TATGAGAAGA TTGTGGAAAA AATACGAGCT TTACAAATGA AAGCTGAAGA CTATGATGTT 
GTTAAGGTGA TCGGAAGAGG GGCTTTTGGA GAAGTGCAGC TGGTTCGCCA TAAAATGACA 
CAGAAGGTTT ATGCAATGAA GCTGCTTAGT AAATTTGAAA TGATCAAGAG ATCAGATTCA 
GCCTTTTTCT GGGAAGAAAG AGATATCATG GCCTTTGCCA ATAGTCCATG GGTGGTTCAG 
CTTTTTTGTG CCTTTCAAGA TGACAAATAC TTGTATATGG TAATGGAATA CATGCCAGGA 
GGAGATCTTG TAAACCTTAT GAGCAATTAT GATGTGCCTG AGAAATGGGC CAAGTTCTAT 
ACAGCCGAAG TTGTTCTTGC TCTTGATGCA ATTCACTCCA TGGGCTTGAT ACACAGAGAT 
GTGAAGCCTG ACAATATGCT ATTAGATAAA CATGGGCACC TGAAGCTGGC AGATTTTGGC 
ACTTGCATGA AAATGGATGA AACAGGTATG GTGCGCTGTG ACACGGCTGT TGGAACCCCC 
GACTACATAT CCCCCGAAGT TCTGAAATCA CAAGGGGGTG ATGGTTATTA TGGACGGGAG 
TGTGACTGGT GGTCCGTAGG AGTTTTCCTT TTTGAGATGC TAGTTGGTGA TACTCCTTTT 
TATGCAGACT CTTTAGTAGG GACATACAGT AAAATTATGG ATCACAAGAA CTCATTACAT 
TTCCCAGATG ATGTAGAAAT CTCTAAGCAT GCAAAGAACC TCATCTGTGC CTTCTTAACT 
GACAGGGACG TGCGACTTGG GAGAAATGGG GTAGAAGAAA TAAAGCATCA CCCTTTCTTT 
AAGAGTGATC AGTGGAATTG GGACAACATC CGAGAGACTG CTGCTCCTGT TGTTCCTGAG 
CTTAGCAGTG ATATAGACAG CAGTAATTTT GATGACATTG AGGATGACAA GGGAGACGTG 
GAAACCTTTC CAATCCCCAA AGCCTTTGTG GGAAACCAGC TGCCTTTTAT AGGATTTACC 
TACTATAGAG ATAATTTGTT GCTAAGTGAC TCCTCTCAGT CTTGCAGAGA AAATGAATCG 
GTGCAATCTA GTAAAAATGA GTTTCAGAAA AAAGTAAGCA AGTTAGAAGA ACAACTCAGC 
AATGAATTGC AAGCCAAAGA TGAACTAGAA CAGAAATACA GGTCTGCTAA TACTCGTTTA 
GAGAAGATAG TGAAAGAGCT AGATGAAGAG ATAACTTCAA GGAAAAACGT AGAGTCTGCA 
GTCAGACAGC TAGAAAGGGA GAAGGCTCTT CTTCAGCATA AGAATACAGA ATACCAGAGA 
AAAGCAGAAC ATGAAGCAGA TAAGAAACGC AATTTGGAAA ATGAGGTTAA CAGTTTGAAA 
GATCAGCTTG AAGATTTAAA AAAGAGGAAT CAGAACTCTC AAATATCCAA TGAGAAAATC 
AATCAACTAC AAAGACAGTT GGATGAGGCC AACTCTTTGC TGCGATCGGA GTCTGAAACT 
GCAGCCAGGT TAAGGAAAAA CCAGACCGAA AGTACAAAGC AAATCCAGCA GTTGGAAGCT 
AATAATCGAG AACTACAGGA TAAGAACTGC CTGCTAGAGA ACGCTAAACT CAAACTGGAG 
AAGGACTTTC TCAATCTTCA GTCAGCTCTA GAATCAGAAA GGAGAGATCG AAGTCATGGA 
TCAGAGATTA TCAGTGATTT ACAAGGTCGA ATATCGAGCC TGGAAGAAGA AGTGAAAAAT 
GGAAAGAGTG CATTAGCCAA ACTAGAAATG GAGAAAAGGC AATTGCAGGA AAAACTTACA 
GATTTAGAAA AGGAAAAGAG CAACATGGAA ATAGATATGA CATACAAATT CAAAGTTATG 
CAGCAGAACC TTGAGCAAGA GGAAGCTGAA CATAAAGCCA CAAAAGCACG ATTGGCAGAC 
AAAAATAAGA TTTATGAATC TATAGAGGAG GCAAAATCTG AAGCCATGAA AGAAATGGAG 
AAGAAACTTT TGGAAGAGAG AGCTTTGAAG CAAAAAGTAG AAAATCGGTT GTTAGAAGCT 
GAAAAGCAGC GTTCCATGTT GGATTGTGAT CTCAAACAAT CACAGCAGAA AATAAATGAA 
CTCCTTAGGC AAAAGGATAA ACTAAATGAA GATGTTAAAA ACTTGACTTT AAAAATAGAA 
CAAGAAACAC AAAAGCGTTG TCTCACTCAA AACGACCTCA AGATGCAAAC ACAACAGGTC 
AACACCTTAA AAATGTCAGA GAAGCAGCTA AAACAGGAGA ATAATCATCT TCAGGAAATC 
AAATTAAGCC TGGAGAAACA AAACAATGAA CTCCGCAAAG AACGTCAAGA TGCAGATGGA 
CAAATGAAAG AGCTTCAAGA CCAACTTGAA GCTGAACAGT ATTTCTCGAC TCTGTATAAG 
ACGCAAGTTC GAGAACTTAA AGAAGAATGT GAGGAAAAGA CCAAACTTTG TAAAGAGATG 
CAGCAAAAGA TACAAGAGTT ACAGGACGAG AGAGATTCCT TGGCAGCACA GCTAGAAATC 
ACTTTGACAA AAGCAGATTC AGAACAACTT GCACGTTCCA TTGCTGAAGA ACAGTACTCC 
GATTTGGAAA AAGAGAAGAT TATGAAGGAA CTGGAGATTA AAGAAATGAT GGCTCGGCAT 
AAACAGGAAC TTGCTGAGAA GGATGCCACC ATAGCCTCTT TGGAGGAAGC AAACAGAACA 
CTAACAAGCG ATGTGGCTAA CCTTGCTAAT GAGAAAGAAG AACTAAACAA CAAACTGAAG 
GAAGAACAAG AACAAATTAC AAAGTTGAAA GAAGAAGAAG CAAATATAGG AAATATTAAA 
GCACAGTTTG AGAAACAACT GCTGAATGAA AGAACACTGA AAACCCAGGC TGTGAATAAA 
TTGGCTGAGA TCATGAATCG GAAGGGTCCA GTCAAACGTG GAGCTGACAC CGATGTACGG 
CGGAAGGAAA AGGAAAATAG GAAACTGCAT ATGGAACTGA AATCTGAACG AGAGAAGTTA 
ACACAAATGA TGATCAAATA TCAGAAAGAA ATCAATGAAA TGCAGGCACA AATAGCAGAA 
GAGAGTCAGA TACGAATTGA ACTCCAGATG ACTCTGGACA GCAAAGACAG TGACATTGAA 
CAGCTTCGGT CACAACTACA ATCACTACAT ATTGGCCTAG ACAACAGTAG CATAGGCAGC 
GGACCTGGAG ATGCTGAGGC AGATGATGGA TTCCCTGAAT CAAGATTGGA AGGCTGGTTA 
TCACTACCTG TTCGAAATAA CACTAAAAAA TTTGGTTGGG TTAAAAAGTA TGTAATTGTA 
AGCAGTAAGA AGATCCTGTT CTATGACAGT GAACAAGATA AAGAACAATC TAATCCCTAT 
ATGGTATTAG ACATAGACAA ACTGTTCCAT GTCCGACCAG TCACCCAGAC TGATGTGTAC 
AGAGCAGATG CCAAGGAAAT TCCAAGGATA TTCCAGATTC TGTATGCTAA TGAAGGAGAG 
AGCAAAAAGG AGCAGGAATT TCCTGTGGAG CCTATGGGAG AGAAGTCAAA TTATATTTGT 
CACAAAGGAC ATGAGTTTAT ACCTACTCTT TATCACTTTC CAACCAATTG TGAAGCTTGT 
ATGAAGCCTC TTTGGCATAT GTTTAAACCT CCTCCTGCTC TAGAATGTCG CCGTTGCCAT 
ATCAAATGTC ACAAAGATCA TATGGATAAA AAAGAAGAGA TTATAGCACC TTGCAAAGTG 
TATTACGATA TTTCAACGGC AAAGAATCTA CTACTGTTAG CTAATTCTAC AGAAGAACAG 
CAAAAATGGG TGAGCCGACT GGTGAAAAAA ATACCCAAGA AACCTCCAGC ACCAGACCCC 
TTTGCTCGAT CTTCTCCCAG AACTTCTATG AAGGTACAGC CAAACCAGTC CATCAGACGA 
CCAAGTCGAC AGCTTCCTCC AAACAAACCA AGATTGCTTG ATCTGGCATT TAAAGACTGG 
GATTGGTCAT TTGATGATGT TGATGATATT GATGATGATG ATGATGATGA TGATGACGAT 
GTTTTTGATT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ROCK2 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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