Rock2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rock2
Entrez Gene ID	19878
Full Name	Rho-associated coiled-coil containing protein kinase 2
Synonyms	B230113H15Rik,ROKalpha,Rho-kinase,Rock-II,Rock2m,mKIAA0619
General protein information	Preferred Names Rho-associated coiled-coil containing protein kinase 2 Names rho-associated protein kinase 2 Rho-associated coiled-coil forming kinage 2 p164 ROCK-2 rho-associated, coiled-coil-containing protein kinase 2 rho-associated, coiled-coil-containing protein kinase II
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 A1.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515019.4	XP_006515082.1	rho-associated protein kinase 2 isoform X1
XM_006515020.4	XP_006515083.1	rho-associated protein kinase 2 isoform X1
XM_006515019.3	XP_006515082.1	rho-associated protein kinase 2 isoform X1
NM_009072.2	NP_033098.2	rho-associated protein kinase 2
XM_006515020.3	XP_006515083.1	rho-associated protein kinase 2 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rock2	NP_033098.2
Drosophila melanogaster (fruit fly)	rok	NP_536796.2
Caenorhabditis elegans (roundworm)	let-502	NP_491440.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000406	XP_310694.5
Pan troglodytes (chimpanzee)	ROCK2	XP_525689.3
Rattus norvegicus (Norway rat)	Rock2	NP_037154.2
Xenopus tropicalis (tropical clawed frog)	rock2	XP_004914525.1
Homo sapiens (human)	ROCK2	NP_004841.2
Macaca mulatta (Rhesus monkey)	ROCK2	XP_001096931.2
Canis lupus familiaris (dog)	ROCK2	XP_540083.4
Danio rerio (zebrafish)	rock2a	NP_777288.1
Bos taurus (cattle)	ROCK2	NP_776877.1
Gallus gallus (chicken)	ROCK2	XP_004935820.1

Gene Ontology
Bibliography

Related articles in PubMed

Fasudil or genetic depletion of ROCK1 or ROCK2 induces anxiety-like behaviors.
Greathouse KM, Henderson BW, Gentry EG, Herskowitz JH
Behavioural brain research373112083(2019 Nov)

ROCK2 promotes ryanodine receptor phosphorylation and arrhythmic calcium release in diabetic cardiomyocytes.
Soliman H, Nyamandi V, Garcia-Patino M, Zhang PC, Lin E, Jia ZP, Tibbits GF, Hove-Madsen L, MacLeod KM
International journal of cardiology28190-98(2019 Apr)

Distinct and complementary functions of rho kinase isoforms ROCK1 and ROCK2 in prefrontal cortex structural plasticity.
Greathouse KM, Boros BD, Deslauriers JF, Henderson BW, Curtis KA, Gentry EG, Herskowitz JH
Brain structure & function223(9)4227-4241(2018 Dec)

Different roles of myocardial ROCK1 and ROCK2 in cardiac dysfunction and postcapillary pulmonary hypertension in mice.
Sunamura S, Satoh K, Kurosawa R, Ohtsuki T, Kikuchi N, Elias-Al-Mamun M, Shimizu T, Ikeda S, Suzuki K, Satoh T, Omura J, Nogi M, Numano K, Siddique MAH, Miyata S, Miura M, Shimokawa H
Proceedings of the National Academy of Sciences of the United States of America115(30)E7129-E7138(2018 07)

Toll-like receptor signaling in macrophages is regulated by extracellular substrate stiffness and Rho-associated coiled-coil kinase (ROCK1/2).
Gruber E, Heyward C, Cameron J, Leifer C
International immunology30(6)267-278(2018 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rock2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rock2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20909	NM_009072.2 Latest version!	Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu47266	XM_006515020.4 Latest version!	Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu47266	XM_006515019.4 Latest version!	Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OMu47266	XM_006515019.3	Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu47266	XM_006515020.3	Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rock2 ( NM_009072.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033098.2 Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), mRNA.

Price & Availability↑

CloneID	OMu20909
Clone ID Related Accession (Same CDS sequence)	NM_009072.2
Accession Version	NM_009072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577808000000
Organism	Mus musculus(house mouse)
Product	rho-associated protein kinase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ127012.1, AK163082.1, U58513.1, AK045517.1, CN536850.1, AK048955.1 and AK153280.1. On Mar 31, 2007 this sequence version replaced NM_009072.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCGGGCG CCGGCCGTCA GAGGAAGCTG GAGGCGCTCA TCCGAGACCC TCGCTCGCCC 
ATCAACGTGG AGAGTCTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAATAAAAA TATAGATAAC TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTTTACAGAT GAAAGCGGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTTCA GTTGGTTCGT CATAAGGCAT CACAGAAGGT TTATGCAATG 
AAGCTTCTTA GTAAATTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTAGTTC AGCTCTTCTG TGCCTTTCAA 
GATGACAGGT ACCTGTACAT GGTGATGGAG TACATGCCAG GCGGAGACCT CGTCAACCTT 
ATGAGTAATT ATGATGTACC TGAAAAGTGG GCCAAGTTTT ACACTGCTGA AGTAGTGCTT 
GCACTGGATG CAATACACTC CATGGGCTTA ATACACAGAG ATGTGAAGCC TGATAACATG 
CTATTGGATA AACATGGACA TCTAAAGTTA GCAGATTTTG GCACATGTAT GAAAATGGAT 
GAAACAGGCA TGGTGCATTG CGACACAGCA GTTGGAACTC CCGACTACAT ATCGCCCGAG 
GTTCTGAAAT CACAAGGAGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTTTGAGAT GTTAGTAGGG GACACTCCAT TTTACGCAGA TTCACTTGTA 
GGAACCTACA GCAAAATTAT GGATCATAAA AATTCACTGT GTTTTCCTGA AGATACAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATATGT GCCTTCTTAA CAGACAGGGA GGTACGACTT 
GGAAGAAATG GGGTAGAAGA AATCAAACAG CATCCTTTCT TTAAGAATGA TCAATGGAAT 
TGGGATAACA TAAGAGAGAC GGCAGCTCCA GTGGTACCTG AGCTCAGCAG TGACATAGAC 
AGCAGCAACT TTGACGACAT TGAGGATGAC AAAGGCGATG TAGAGACCTT CCCGATTCCT 
AAAGCTTTTG TGGGAAATCA GCTGCCTTTT ATAGGATTTA CCTACTTTAG AGAAAATTTG 
TTATTAAGTG ACTCTCCACC TTGTAGAGAA AATGATGCAA TACAAACAAG GAAAAGTGAA 
GAAAGTCAGG AGATTCAGAA AAAATTATAT GCACTAGAAG AACACCTTAG CAGTGAGGTG 
CAAGCCAAGG AGGAGCTGGA ACAGAAGTGC AAATCTATTA ATACTCGCCT AGAGAAAACA 
GCAAAGGAGC TAGAAGAAGA GATTACCTTA CGGAAAAGTG TGGAATCAAC ATTAAGACAG 
CTGGAAAGAG AAAAGGCCCT TCTTCAGCAC AAAAATGCAG AGTATCAGCG GAAAGCTGAT 
CATGAAGCCG ACAAGAAGCG GAACTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATCTGA AGAAAAGGAA CCAGAGCTCT CAGATATCCA CTGAGAAGGT CAATCAGCTC 
CAGAAACAAC TGGATGAAGC TAATGCTTTG CTGAGAACAG AGTCTGATAC TGCAGCACGG 
TTAAGAAAAA CCCAGGCAGA AAGTTCCAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTTGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTTT AGAATCTGAA AGAAGGGACC GGACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATCTGGG TTGGAAGAAG ATTTGAAGAC TGGCAAAGCC 
TTACTAGCAA AAGTAGAGCT GGAGAAGAGA CAGCTGCAGG AGAAGCTCAC TGACTTAGAG 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATATCAAC TGAAAGTTAT ACAGCAGAGT 
TTAGAACAAG AAGAAGCTGA ACACAAGACC ACAAAAGCAC GGCTAGCGGA TAAAAATAAG 
ATCTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTG 
TTGGAAGAGA GGTCTTTAAA GCAGAAAGTG GAGAACCTGC TCCTGGAAGC CGAGAAAAGA 
TGCTCTATAT TGGACTGTGA CCTCAAACAG TCTCAGCAGA AGCTAAATGA GCTGCTGAAG 
CAGAAAGATG TGCTGAACGA GGATGTTAGA AATTTGACAT TAAAAATAGA GCAGGAAACT 
CAGAAGCGTT GTCTTATGCA AAATGACCTG AAGATGCAGA CCCAACAAGT TAACACCCTA 
AAAATGTCAG AAAAGCAAAT AAAACAAGAA AATAATCATC TTATGGAAAT GAAAATGAAC 
TTGGAAAAGC AAAATACTGA ACTTCGAAAA GAGCGGCAGG ATGCAGATGG GCAGATGAAG 
GAACTGCAAG ACCAACTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAAGTT 
AGAGAACTTA AAGAAGAAAA TGAAGAGAAG ACAAAACTTT GTAAAGAGTT GCAACAGAAG 
AAGCAGGATT TACAGGATGA AAGAGACTCT TTGGCTGCCC AGCTGGAAAT TACCCTTACC 
AAAGCAGATT CTGAGCAGCT GGCTCGTTCC ATTGCTGAAG AACAATACTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAAGAA 
CTTACTGAAA AGGATACTAC AATTGCATCT CTTGAAGAAA CAAATAGGAC ACTAACTAGC 
GATGTTGCAA ATCTTGCAAA TGAGAAAGAA GAATTAAACA ACAAGCTGAA AGATAGCCAA 
GAACAACTGT CCAAGTTGAA AGATGAAGAG ATGAGTGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TTCTGAATGA GAGAACTCTC AAGACTCAAG CTGTGAATAA GTTGGCGGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAGTGACA CAGATGTGCG AAGAAAAGAG 
AAGGAAAACA GAAAGCTACA TATGGAGCTT AAGTCTGAAC GTGAAAAATT GACACAACAG 
ATGATCAAAT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGCCAA 
ATTCGAATAG AACTCCAGAT GACCCTGGAC AGTAAAGACA GCGACATTGA ACAGCTGCGG 
TCACAGCTCC AGGCCTTGCA TATTGGTATG GACAGTTCCA GTATAGGCAG TGGACCAGGC 
GATGCTGAGC CTGATGATGG ATTTCCAGAA TCAAGATTAG AAGGATGGTT GTCATTGCCT 
GTGCGCAACA ACACTAAAAA GTTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGCAAG 
AAGATTCTTT TTTATGACAG CGAACAAGAT AAAGAGCAGT CCAACCCTTA CATGGTGTTA 
GACATAGACA AGCTATTTCA CGTCCGACCT GTTACCCAGA CAGATGTATA CAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTTCAGATT CTATATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACCAGAGT TTCCAGTGGA ACCAGTGGGA GAAAAATCTA ATTATATTTG TCACAAAGGA 
CATGAGTTTA TTCCTACTCT TTATCACTTC CCAACCAACT GTGAGGCATG TATGAAGCCG 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT CTAGAGTGCC GTAGGTGCCA CATTAAATGT 
CATAAAGATC ACATGGACAA GAAGGAAGAG ATTATAGCGC CCTGCAAAGT TTATTATGAT 
ATATCGTCGG CAAAGAATCT GTTGTTATTA GCAAATTCAA CAGAAGAACA GCAGAAATGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCTCCAG CTCCAGACCC TTTTGCCCGA 
TCATCCCCTA GAACTTCAAT GAAAATACAA CAAAACCAGT CCATTCGGCG GCCCAGTCGA 
CAGCTTGCTC CAAACAAGCC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033098.2
CDS	213..4379
Translation	MSRPPPTGKMPGAPETAPGDGAGAGRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITLRKSVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQLQKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRISGLEEDLKTGKALLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQSQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMNLEKQNTELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDTTIASLEETNRTLTSDVANLANEKEELNNKLKDSQEQLSKLKDEEMSAAAIKAQFEKQLLNERTLKTQAVNKLAEIMNRKEPVKRGSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGMDSSSIGSGPGDAEPDDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	NM_009072.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009072.2

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAAATG CCCGGCGCCC CCGAGACCGC GCCGGGGGAC 
GGGGCGGGCG CCGGCCGTCA GAGGAAGCTG GAGGCGCTCA TCCGAGACCC TCGCTCGCCC 
ATCAACGTGG AGAGTCTGCT GGATGGCTTA AATTCCTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAATAAAAA TATAGATAAC TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATCAGAG GTTTACAGAT GAAAGCGGAA GACTATGATG TTGTAAAAGT TATTGGAAGA 
GGTGCTTTTG GTGAAGTTCA GTTGGTTCGT CATAAGGCAT CACAGAAGGT TTATGCAATG 
AAGCTTCTTA GTAAATTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAAGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTAGTTC AGCTCTTCTG TGCCTTTCAA 
GATGACAGGT ACCTGTACAT GGTGATGGAG TACATGCCAG GCGGAGACCT CGTCAACCTT 
ATGAGTAATT ATGATGTACC TGAAAAGTGG GCCAAGTTTT ACACTGCTGA AGTAGTGCTT 
GCACTGGATG CAATACACTC CATGGGCTTA ATACACAGAG ATGTGAAGCC TGATAACATG 
CTATTGGATA AACATGGACA TCTAAAGTTA GCAGATTTTG GCACATGTAT GAAAATGGAT 
GAAACAGGCA TGGTGCATTG CGACACAGCA GTTGGAACTC CCGACTACAT ATCGCCCGAG 
GTTCTGAAAT CACAAGGAGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCTGTA 
GGTGTTTTCC TTTTTGAGAT GTTAGTAGGG GACACTCCAT TTTACGCAGA TTCACTTGTA 
GGAACCTACA GCAAAATTAT GGATCATAAA AATTCACTGT GTTTTCCTGA AGATACAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATATGT GCCTTCTTAA CAGACAGGGA GGTACGACTT 
GGAAGAAATG GGGTAGAAGA AATCAAACAG CATCCTTTCT TTAAGAATGA TCAATGGAAT 
TGGGATAACA TAAGAGAGAC GGCAGCTCCA GTGGTACCTG AGCTCAGCAG TGACATAGAC 
AGCAGCAACT TTGACGACAT TGAGGATGAC AAAGGCGATG TAGAGACCTT CCCGATTCCT 
AAAGCTTTTG TGGGAAATCA GCTGCCTTTT ATAGGATTTA CCTACTTTAG AGAAAATTTG 
TTATTAAGTG ACTCTCCACC TTGTAGAGAA AATGATGCAA TACAAACAAG GAAAAGTGAA 
GAAAGTCAGG AGATTCAGAA AAAATTATAT GCACTAGAAG AACACCTTAG CAGTGAGGTG 
CAAGCCAAGG AGGAGCTGGA ACAGAAGTGC AAATCTATTA ATACTCGCCT AGAGAAAACA 
GCAAAGGAGC TAGAAGAAGA GATTACCTTA CGGAAAAGTG TGGAATCAAC ATTAAGACAG 
CTGGAAAGAG AAAAGGCCCT TCTTCAGCAC AAAAATGCAG AGTATCAGCG GAAAGCTGAT 
CATGAAGCCG ACAAGAAGCG GAACTTGGAA AATGATGTTA ACAGCTTAAA AGATCAACTT 
GAAGATCTGA AGAAAAGGAA CCAGAGCTCT CAGATATCCA CTGAGAAGGT CAATCAGCTC 
CAGAAACAAC TGGATGAAGC TAATGCTTTG CTGAGAACAG AGTCTGATAC TGCAGCACGG 
TTAAGAAAAA CCCAGGCAGA AAGTTCCAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ATAAAAACTG CCTGCTTGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGCTTT AGAATCTGAA AGAAGGGACC GGACCCATGG ATCAGAGATA 
ATTAATGATT TACAAGGTAG AATATCTGGG TTGGAAGAAG ATTTGAAGAC TGGCAAAGCC 
TTACTAGCAA AAGTAGAGCT GGAGAAGAGA CAGCTGCAGG AGAAGCTCAC TGACTTAGAG 
AAGGAAAAAA GCAACATGGA AATAGATATG ACATATCAAC TGAAAGTTAT ACAGCAGAGT 
TTAGAACAAG AAGAAGCTGA ACACAAGACC ACAAAAGCAC GGCTAGCGGA TAAAAATAAG 
ATCTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAGAAGCTG 
TTGGAAGAGA GGTCTTTAAA GCAGAAAGTG GAGAACCTGC TCCTGGAAGC CGAGAAAAGA 
TGCTCTATAT TGGACTGTGA CCTCAAACAG TCTCAGCAGA AGCTAAATGA GCTGCTGAAG 
CAGAAAGATG TGCTGAACGA GGATGTTAGA AATTTGACAT TAAAAATAGA GCAGGAAACT 
CAGAAGCGTT GTCTTATGCA AAATGACCTG AAGATGCAGA CCCAACAAGT TAACACCCTA 
AAAATGTCAG AAAAGCAAAT AAAACAAGAA AATAATCATC TTATGGAAAT GAAAATGAAC 
TTGGAAAAGC AAAATACTGA ACTTCGAAAA GAGCGGCAGG ATGCAGATGG GCAGATGAAG 
GAACTGCAAG ACCAACTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAAGTT 
AGAGAACTTA AAGAAGAAAA TGAAGAGAAG ACAAAACTTT GTAAAGAGTT GCAACAGAAG 
AAGCAGGATT TACAGGATGA AAGAGACTCT TTGGCTGCCC AGCTGGAAAT TACCCTTACC 
AAAGCAGATT CTGAGCAGCT GGCTCGTTCC ATTGCTGAAG AACAATACTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTAGACA CAAACAAGAA 
CTTACTGAAA AGGATACTAC AATTGCATCT CTTGAAGAAA CAAATAGGAC ACTAACTAGC 
GATGTTGCAA ATCTTGCAAA TGAGAAAGAA GAATTAAACA ACAAGCTGAA AGATAGCCAA 
GAACAACTGT CCAAGTTGAA AGATGAAGAG ATGAGTGCAG CAGCTATTAA AGCACAGTTT 
GAGAAGCAGC TTCTGAATGA GAGAACTCTC AAGACTCAAG CTGTGAATAA GTTGGCGGAG 
ATCATGAATC GAAAAGAACC TGTCAAGCGT GGTAGTGACA CAGATGTGCG AAGAAAAGAG 
AAGGAAAACA GAAAGCTACA TATGGAGCTT AAGTCTGAAC GTGAAAAATT GACACAACAG 
ATGATCAAAT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGCCAA 
ATTCGAATAG AACTCCAGAT GACCCTGGAC AGTAAAGACA GCGACATTGA ACAGCTGCGG 
TCACAGCTCC AGGCCTTGCA TATTGGTATG GACAGTTCCA GTATAGGCAG TGGACCAGGC 
GATGCTGAGC CTGATGATGG ATTTCCAGAA TCAAGATTAG AAGGATGGTT GTCATTGCCT 
GTGCGCAACA ACACTAAAAA GTTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGCAAG 
AAGATTCTTT TTTATGACAG CGAACAAGAT AAAGAGCAGT CCAACCCTTA CATGGTGTTA 
GACATAGACA AGCTATTTCA CGTCCGACCT GTTACCCAGA CAGATGTATA CAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTTCAGATT CTATATGCCA ATGAAGGAGA AAGTAAGAAG 
GAACCAGAGT TTCCAGTGGA ACCAGTGGGA GAAAAATCTA ATTATATTTG TCACAAAGGA 
CATGAGTTTA TTCCTACTCT TTATCACTTC CCAACCAACT GTGAGGCATG TATGAAGCCG 
CTGTGGCACA TGTTTAAGCC TCCTCCTGCT CTAGAGTGCC GTAGGTGCCA CATTAAATGT 
CATAAAGATC ACATGGACAA GAAGGAAGAG ATTATAGCGC CCTGCAAAGT TTATTATGAT 
ATATCGTCGG CAAAGAATCT GTTGTTATTA GCAAATTCAA CAGAAGAACA GCAGAAATGG 
GTTAGTCGGT TGGTGAAAAA GATACCTAAA AAGCCTCCAG CTCCAGACCC TTTTGCCCGA 
TCATCCCCTA GAACTTCAAT GAAAATACAA CAAAACCAGT CCATTCGGCG GCCCAGTCGA 
CAGCTTGCTC CAAACAAGCC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rock2 ( XM_006515019.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515082.1 Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47266
Clone ID Related Accession (Same CDS sequence)	XM_006515019.4 , XM_006515020.4 , XM_006515019.3 , XM_006515020.3
Accession Version	XM_006515019.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515019.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTAGTAAATT TGAAATGATA AAAAGATCAG ATTCTGCTTT TTTCTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAACAGT CCCTGGGTAG TTCAGCTCTT CTGTGCCTTT 
CAAGATGACA GGTACCTGTA CATGGTGATG GAGTACATGC CAGGCGGAGA CCTCGTCAAC 
CTTATGAGTA ATTATGATGT ACCTGAAAAG TGGGCCAAGT TTTACACTGC TGAAGTAGTG 
CTTGCACTGG ATGCAATACA CTCCATGGGC TTAATACACA GAGATGTGAA GCCTGATAAC 
ATGCTATTGG ATAAACATGG ACATCTAAAG TTAGCAGATT TTGGCACATG TATGAAAATG 
GATGAAACAG GCATGGTGCA TTGCGACACA GCAGTTGGAA CTCCCGACTA CATATCGCCC 
GAGGTTCTGA AATCACAAGG AGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTTTGA GATGTTAGTA GGGGACACTC CATTTTACGC AGATTCACTT 
GTAGGAACCT ACAGCAAAAT TATGGATCAT AAAAATTCAC TGTGTTTTCC TGAAGATACA 
GAAATTTCTA AACATGCGAA GAATCTCATA TGTGCCTTCT TAACAGACAG GGAGGTACGA 
CTTGGAAGAA ATGGGGTAGA AGAAATCAAA CAGCATCCTT TCTTTAAGAA TGATCAATGG 
AATTGGGATA ACATAAGAGA GACGGCAGCT CCAGTGGTAC CTGAGCTCAG CAGTGACATA 
GACAGCAGCA ACTTTGACGA CATTGAGGAT GACAAAGGCG ATGTAGAGAC CTTCCCGATT 
CCTAAAGCTT TTGTGGGAAA TCAGCTGCCT TTTATAGGAT TTACCTACTT TAGAGAAAAT 
TTGTTATTAA GTGACTCTCC ACCTTGTAGA GAAAATGATG CAATACAAAC AAGGAAAAGT 
GAAGAAAGTC AGGAGATTCA GAAAAAATTA TATGCACTAG AAGAACACCT TAGCAGTGAG 
GTGCAAGCCA AGGAGGAGCT GGAACAGAAG TGCAAATCTA TTAATACTCG CCTAGAGAAA 
ACAGCAAAGG AGCTAGAAGA AGAGATTACC TTACGGAAAA GTGTGGAATC AACATTAAGA 
CAGCTGGAAA GAGAAAAGGC CCTTCTTCAG CACAAAAATG CAGAGTATCA GCGGAAAGCT 
GATCATGAAG CCGACAAGAA GCGGAACTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATC TGAAGAAAAG GAACCAGAGC TCTCAGATAT CCACTGAGAA GGTCAATCAG 
CTCCAGAAAC AACTGGATGA AGCTAATGCT TTGCTGAGAA CAGAGTCTGA TACTGCAGCA 
CGGTTAAGAA AAACCCAGGC AGAAAGTTCC AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTT GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TTTAGAATCT GAAAGAAGGG ACCGGACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATCT GGGTTGGAAG AAGATTTGAA GACTGGCAAA 
GCCTTACTAG CAAAAGTAGA GCTGGAGAAG AGACAGCTGC AGGAGAAGCT CACTGACTTA 
GAGAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATATC AACTGAAAGT TATACAGCAG 
AGTTTAGAAC AAGAAGAAGC TGAACACAAG ACCACAAAAG CACGGCTAGC GGATAAAAAT 
AAGATCTATG AATCCATTGA AGAAGCTAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTGTTGGAAG AGAGGTCTTT AAAGCAGAAA GTGGAGAACC TGCTCCTGGA AGCCGAGAAA 
AGATGCTCTA TATTGGACTG TGACCTCAAA CAGTCTCAGC AGAAGCTAAA TGAGCTGCTG 
AAGCAGAAAG ATGTGCTGAA CGAGGATGTT AGAAATTTGA CATTAAAAAT AGAGCAGGAA 
ACTCAGAAGC GTTGTCTTAT GCAAAATGAC CTGAAGATGC AGACCCAACA AGTTAACACC 
CTAAAAATGT CAGAAAAGCA AATAAAACAA GAAAATAATC ATCTTATGGA AATGAAAATG 
AACTTGGAAA AGCAAAATAC TGAACTTCGA AAAGAGCGGC AGGATGCAGA TGGGCAGATG 
AAGGAACTGC AAGACCAACT TGAAGCAGAG CAGTATTTCT CAACCCTCTA TAAAACACAA 
GTTAGAGAAC TTAAAGAAGA AAATGAAGAG AAGACAAAAC TTTGTAAAGA GTTGCAACAG 
AAGAAGCAGG ATTTACAGGA TGAAAGAGAC TCTTTGGCTG CCCAGCTGGA AATTACCCTT 
ACCAAAGCAG ATTCTGAGCA GCTGGCTCGT TCCATTGCTG AAGAACAATA CTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAA 
GAACTTACTG AAAAGGATAC TACAATTGCA TCTCTTGAAG AAACAAATAG GACACTAACT 
AGCGATGTTG CAAATCTTGC AAATGAGAAA GAAGAATTAA ACAACAAGCT GAAAGATAGC 
CAAGAACAAC TGTCCAAGTT GAAAGATGAA GAGATGAGTG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTTCTGAA TGAGAGAACT CTCAAGACTC AAGCTGTGAA TAAGTTGGCG 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAGTG ACACAGATGT GCGAAGAAAA 
GAGAAGGAAA ACAGAAAGCT ACATATGGAG CTTAAGTCTG AACGTGAAAA ATTGACACAA 
CAGATGATCA AATATCAGAA AGAACTGAAT GAAATGCAGG CTCAAATAGC TGAAGAGAGC 
CAAATTCGAA TAGAACTCCA GATGACCCTG GACAGTAAAG ACAGCGACAT TGAACAGCTG 
CGGTCACAGC TCCAGGCCTT GCATATTGGT ATGGACAGTT CCAGTATAGG CAGTGGACCA 
GGCGATGCTG AGCCTGATGA TGGATTTCCA GAATCAAGAT TAGAAGGATG GTTGTCATTG 
CCTGTGCGCA ACAACACTAA AAAGTTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGC 
AAGAAGATTC TTTTTTATGA CAGCGAACAA GATAAAGAGC AGTCCAACCC TTACATGGTG 
TTAGACATAG ACAAGCTATT TCACGTCCGA CCTGTTACCC AGACAGATGT ATACAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTTCAG ATTCTATATG CCAATGAAGG AGAAAGTAAG 
AAGGAACCAG AGTTTCCAGT GGAACCAGTG GGAGAAAAAT CTAATTATAT TTGTCACAAA 
GGACATGAGT TTATTCCTAC TCTTTATCAC TTCCCAACCA ACTGTGAGGC ATGTATGAAG 
CCGCTGTGGC ACATGTTTAA GCCTCCTCCT GCTCTAGAGT GCCGTAGGTG CCACATTAAA 
TGTCATAAAG ATCACATGGA CAAGAAGGAA GAGATTATAG CGCCCTGCAA AGTTTATTAT 
GATATATCGT CGGCAAAGAA TCTGTTGTTA TTAGCAAATT CAACAGAAGA ACAGCAGAAA 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCTC CAGCTCCAGA CCCTTTTGCC 
CGATCATCCC CTAGAACTTC AATGAAAATA CAACAAAACC AGTCCATTCG GCGGCCCAGT 
CGACAGCTTG CTCCAAACAA GCCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515082.1
CDS	398..4207
Translation	MKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITLRKSVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQLQKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRISGLEEDLKTGKALLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQSQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMNLEKQNTELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDTTIASLEETNRTLTSDVANLANEKEELNNKLKDSQEQLSKLKDEEMSAAAIKAQFEKQLLNERTLKTQAVNKLAEIMNRKEPVKRGSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGMDSSSIGSGPGDAEPDDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	XM_006515019.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515019.4

ORF Insert Sequence:

ATGAAGCTTC TTAGTAAATT TGAAATGATA AAAAGATCAG ATTCTGCTTT TTTCTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAACAGT CCCTGGGTAG TTCAGCTCTT CTGTGCCTTT 
CAAGATGACA GGTACCTGTA CATGGTGATG GAGTACATGC CAGGCGGAGA CCTCGTCAAC 
CTTATGAGTA ATTATGATGT ACCTGAAAAG TGGGCCAAGT TTTACACTGC TGAAGTAGTG 
CTTGCACTGG ATGCAATACA CTCCATGGGC TTAATACACA GAGATGTGAA GCCTGATAAC 
ATGCTATTGG ATAAACATGG ACATCTAAAG TTAGCAGATT TTGGCACATG TATGAAAATG 
GATGAAACAG GCATGGTGCA TTGCGACACA GCAGTTGGAA CTCCCGACTA CATATCGCCC 
GAGGTTCTGA AATCACAAGG AGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTTTGA GATGTTAGTA GGGGACACTC CATTTTACGC AGATTCACTT 
GTAGGAACCT ACAGCAAAAT TATGGATCAT AAAAATTCAC TGTGTTTTCC TGAAGATACA 
GAAATTTCTA AACATGCGAA GAATCTCATA TGTGCCTTCT TAACAGACAG GGAGGTACGA 
CTTGGAAGAA ATGGGGTAGA AGAAATCAAA CAGCATCCTT TCTTTAAGAA TGATCAATGG 
AATTGGGATA ACATAAGAGA GACGGCAGCT CCAGTGGTAC CTGAGCTCAG CAGTGACATA 
GACAGCAGCA ACTTTGACGA CATTGAGGAT GACAAAGGCG ATGTAGAGAC CTTCCCGATT 
CCTAAAGCTT TTGTGGGAAA TCAGCTGCCT TTTATAGGAT TTACCTACTT TAGAGAAAAT 
TTGTTATTAA GTGACTCTCC ACCTTGTAGA GAAAATGATG CAATACAAAC AAGGAAAAGT 
GAAGAAAGTC AGGAGATTCA GAAAAAATTA TATGCACTAG AAGAACACCT TAGCAGTGAG 
GTGCAAGCCA AGGAGGAGCT GGAACAGAAG TGCAAATCTA TTAATACTCG CCTAGAGAAA 
ACAGCAAAGG AGCTAGAAGA AGAGATTACC TTACGGAAAA GTGTGGAATC AACATTAAGA 
CAGCTGGAAA GAGAAAAGGC CCTTCTTCAG CACAAAAATG CAGAGTATCA GCGGAAAGCT 
GATCATGAAG CCGACAAGAA GCGGAACTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATC TGAAGAAAAG GAACCAGAGC TCTCAGATAT CCACTGAGAA GGTCAATCAG 
CTCCAGAAAC AACTGGATGA AGCTAATGCT TTGCTGAGAA CAGAGTCTGA TACTGCAGCA 
CGGTTAAGAA AAACCCAGGC AGAAAGTTCC AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTT GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TTTAGAATCT GAAAGAAGGG ACCGGACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATCT GGGTTGGAAG AAGATTTGAA GACTGGCAAA 
GCCTTACTAG CAAAAGTAGA GCTGGAGAAG AGACAGCTGC AGGAGAAGCT CACTGACTTA 
GAGAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATATC AACTGAAAGT TATACAGCAG 
AGTTTAGAAC AAGAAGAAGC TGAACACAAG ACCACAAAAG CACGGCTAGC GGATAAAAAT 
AAGATCTATG AATCCATTGA AGAAGCTAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTGTTGGAAG AGAGGTCTTT AAAGCAGAAA GTGGAGAACC TGCTCCTGGA AGCCGAGAAA 
AGATGCTCTA TATTGGACTG TGACCTCAAA CAGTCTCAGC AGAAGCTAAA TGAGCTGCTG 
AAGCAGAAAG ATGTGCTGAA CGAGGATGTT AGAAATTTGA CATTAAAAAT AGAGCAGGAA 
ACTCAGAAGC GTTGTCTTAT GCAAAATGAC CTGAAGATGC AGACCCAACA AGTTAACACC 
CTAAAAATGT CAGAAAAGCA AATAAAACAA GAAAATAATC ATCTTATGGA AATGAAAATG 
AACTTGGAAA AGCAAAATAC TGAACTTCGA AAAGAGCGGC AGGATGCAGA TGGGCAGATG 
AAGGAACTGC AAGACCAACT TGAAGCAGAG CAGTATTTCT CAACCCTCTA TAAAACACAA 
GTTAGAGAAC TTAAAGAAGA AAATGAAGAG AAGACAAAAC TTTGTAAAGA GTTGCAACAG 
AAGAAGCAGG ATTTACAGGA TGAAAGAGAC TCTTTGGCTG CCCAGCTGGA AATTACCCTT 
ACCAAAGCAG ATTCTGAGCA GCTGGCTCGT TCCATTGCTG AAGAACAATA CTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAA 
GAACTTACTG AAAAGGATAC TACAATTGCA TCTCTTGAAG AAACAAATAG GACACTAACT 
AGCGATGTTG CAAATCTTGC AAATGAGAAA GAAGAATTAA ACAACAAGCT GAAAGATAGC 
CAAGAACAAC TGTCCAAGTT GAAAGATGAA GAGATGAGTG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTTCTGAA TGAGAGAACT CTCAAGACTC AAGCTGTGAA TAAGTTGGCG 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAGTG ACACAGATGT GCGAAGAAAA 
GAGAAGGAAA ACAGAAAGCT ACATATGGAG CTTAAGTCTG AACGTGAAAA ATTGACACAA 
CAGATGATCA AATATCAGAA AGAACTGAAT GAAATGCAGG CTCAAATAGC TGAAGAGAGC 
CAAATTCGAA TAGAACTCCA GATGACCCTG GACAGTAAAG ACAGCGACAT TGAACAGCTG 
CGGTCACAGC TCCAGGCCTT GCATATTGGT ATGGACAGTT CCAGTATAGG CAGTGGACCA 
GGCGATGCTG AGCCTGATGA TGGATTTCCA GAATCAAGAT TAGAAGGATG GTTGTCATTG 
CCTGTGCGCA ACAACACTAA AAAGTTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGC 
AAGAAGATTC TTTTTTATGA CAGCGAACAA GATAAAGAGC AGTCCAACCC TTACATGGTG 
TTAGACATAG ACAAGCTATT TCACGTCCGA CCTGTTACCC AGACAGATGT ATACAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTTCAG ATTCTATATG CCAATGAAGG AGAAAGTAAG 
AAGGAACCAG AGTTTCCAGT GGAACCAGTG GGAGAAAAAT CTAATTATAT TTGTCACAAA 
GGACATGAGT TTATTCCTAC TCTTTATCAC TTCCCAACCA ACTGTGAGGC ATGTATGAAG 
CCGCTGTGGC ACATGTTTAA GCCTCCTCCT GCTCTAGAGT GCCGTAGGTG CCACATTAAA 
TGTCATAAAG ATCACATGGA CAAGAAGGAA GAGATTATAG CGCCCTGCAA AGTTTATTAT 
GATATATCGT CGGCAAAGAA TCTGTTGTTA TTAGCAAATT CAACAGAAGA ACAGCAGAAA 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCTC CAGCTCCAGA CCCTTTTGCC 
CGATCATCCC CTAGAACTTC AATGAAAATA CAACAAAACC AGTCCATTCG GCGGCCCAGT 
CGACAGCTTG CTCCAAACAA GCCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rock2 ( XM_006515020.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515083.1 Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X2, mRNA.

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CloneID	OMu47266
Clone ID Related Accession (Same CDS sequence)	XM_006515019.4 , XM_006515020.4 , XM_006515019.3 , XM_006515020.3
Accession Version	XM_006515020.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515020.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTAGTAAATT TGAAATGATA AAAAGATCAG ATTCTGCTTT TTTCTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAACAGT CCCTGGGTAG TTCAGCTCTT CTGTGCCTTT 
CAAGATGACA GGTACCTGTA CATGGTGATG GAGTACATGC CAGGCGGAGA CCTCGTCAAC 
CTTATGAGTA ATTATGATGT ACCTGAAAAG TGGGCCAAGT TTTACACTGC TGAAGTAGTG 
CTTGCACTGG ATGCAATACA CTCCATGGGC TTAATACACA GAGATGTGAA GCCTGATAAC 
ATGCTATTGG ATAAACATGG ACATCTAAAG TTAGCAGATT TTGGCACATG TATGAAAATG 
GATGAAACAG GCATGGTGCA TTGCGACACA GCAGTTGGAA CTCCCGACTA CATATCGCCC 
GAGGTTCTGA AATCACAAGG AGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTTTGA GATGTTAGTA GGGGACACTC CATTTTACGC AGATTCACTT 
GTAGGAACCT ACAGCAAAAT TATGGATCAT AAAAATTCAC TGTGTTTTCC TGAAGATACA 
GAAATTTCTA AACATGCGAA GAATCTCATA TGTGCCTTCT TAACAGACAG GGAGGTACGA 
CTTGGAAGAA ATGGGGTAGA AGAAATCAAA CAGCATCCTT TCTTTAAGAA TGATCAATGG 
AATTGGGATA ACATAAGAGA GACGGCAGCT CCAGTGGTAC CTGAGCTCAG CAGTGACATA 
GACAGCAGCA ACTTTGACGA CATTGAGGAT GACAAAGGCG ATGTAGAGAC CTTCCCGATT 
CCTAAAGCTT TTGTGGGAAA TCAGCTGCCT TTTATAGGAT TTACCTACTT TAGAGAAAAT 
TTGTTATTAA GTGACTCTCC ACCTTGTAGA GAAAATGATG CAATACAAAC AAGGAAAAGT 
GAAGAAAGTC AGGAGATTCA GAAAAAATTA TATGCACTAG AAGAACACCT TAGCAGTGAG 
GTGCAAGCCA AGGAGGAGCT GGAACAGAAG TGCAAATCTA TTAATACTCG CCTAGAGAAA 
ACAGCAAAGG AGCTAGAAGA AGAGATTACC TTACGGAAAA GTGTGGAATC AACATTAAGA 
CAGCTGGAAA GAGAAAAGGC CCTTCTTCAG CACAAAAATG CAGAGTATCA GCGGAAAGCT 
GATCATGAAG CCGACAAGAA GCGGAACTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATC TGAAGAAAAG GAACCAGAGC TCTCAGATAT CCACTGAGAA GGTCAATCAG 
CTCCAGAAAC AACTGGATGA AGCTAATGCT TTGCTGAGAA CAGAGTCTGA TACTGCAGCA 
CGGTTAAGAA AAACCCAGGC AGAAAGTTCC AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTT GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TTTAGAATCT GAAAGAAGGG ACCGGACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATCT GGGTTGGAAG AAGATTTGAA GACTGGCAAA 
GCCTTACTAG CAAAAGTAGA GCTGGAGAAG AGACAGCTGC AGGAGAAGCT CACTGACTTA 
GAGAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATATC AACTGAAAGT TATACAGCAG 
AGTTTAGAAC AAGAAGAAGC TGAACACAAG ACCACAAAAG CACGGCTAGC GGATAAAAAT 
AAGATCTATG AATCCATTGA AGAAGCTAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTGTTGGAAG AGAGGTCTTT AAAGCAGAAA GTGGAGAACC TGCTCCTGGA AGCCGAGAAA 
AGATGCTCTA TATTGGACTG TGACCTCAAA CAGTCTCAGC AGAAGCTAAA TGAGCTGCTG 
AAGCAGAAAG ATGTGCTGAA CGAGGATGTT AGAAATTTGA CATTAAAAAT AGAGCAGGAA 
ACTCAGAAGC GTTGTCTTAT GCAAAATGAC CTGAAGATGC AGACCCAACA AGTTAACACC 
CTAAAAATGT CAGAAAAGCA AATAAAACAA GAAAATAATC ATCTTATGGA AATGAAAATG 
AACTTGGAAA AGCAAAATAC TGAACTTCGA AAAGAGCGGC AGGATGCAGA TGGGCAGATG 
AAGGAACTGC AAGACCAACT TGAAGCAGAG CAGTATTTCT CAACCCTCTA TAAAACACAA 
GTTAGAGAAC TTAAAGAAGA AAATGAAGAG AAGACAAAAC TTTGTAAAGA GTTGCAACAG 
AAGAAGCAGG ATTTACAGGA TGAAAGAGAC TCTTTGGCTG CCCAGCTGGA AATTACCCTT 
ACCAAAGCAG ATTCTGAGCA GCTGGCTCGT TCCATTGCTG AAGAACAATA CTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAA 
GAACTTACTG AAAAGGATAC TACAATTGCA TCTCTTGAAG AAACAAATAG GACACTAACT 
AGCGATGTTG CAAATCTTGC AAATGAGAAA GAAGAATTAA ACAACAAGCT GAAAGATAGC 
CAAGAACAAC TGTCCAAGTT GAAAGATGAA GAGATGAGTG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTTCTGAA TGAGAGAACT CTCAAGACTC AAGCTGTGAA TAAGTTGGCG 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAGTG ACACAGATGT GCGAAGAAAA 
GAGAAGGAAA ACAGAAAGCT ACATATGGAG CTTAAGTCTG AACGTGAAAA ATTGACACAA 
CAGATGATCA AATATCAGAA AGAACTGAAT GAAATGCAGG CTCAAATAGC TGAAGAGAGC 
CAAATTCGAA TAGAACTCCA GATGACCCTG GACAGTAAAG ACAGCGACAT TGAACAGCTG 
CGGTCACAGC TCCAGGCCTT GCATATTGGT ATGGACAGTT CCAGTATAGG CAGTGGACCA 
GGCGATGCTG AGCCTGATGA TGGATTTCCA GAATCAAGAT TAGAAGGATG GTTGTCATTG 
CCTGTGCGCA ACAACACTAA AAAGTTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGC 
AAGAAGATTC TTTTTTATGA CAGCGAACAA GATAAAGAGC AGTCCAACCC TTACATGGTG 
TTAGACATAG ACAAGCTATT TCACGTCCGA CCTGTTACCC AGACAGATGT ATACAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTTCAG ATTCTATATG CCAATGAAGG AGAAAGTAAG 
AAGGAACCAG AGTTTCCAGT GGAACCAGTG GGAGAAAAAT CTAATTATAT TTGTCACAAA 
GGACATGAGT TTATTCCTAC TCTTTATCAC TTCCCAACCA ACTGTGAGGC ATGTATGAAG 
CCGCTGTGGC ACATGTTTAA GCCTCCTCCT GCTCTAGAGT GCCGTAGGTG CCACATTAAA 
TGTCATAAAG ATCACATGGA CAAGAAGGAA GAGATTATAG CGCCCTGCAA AGTTTATTAT 
GATATATCGT CGGCAAAGAA TCTGTTGTTA TTAGCAAATT CAACAGAAGA ACAGCAGAAA 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCTC CAGCTCCAGA CCCTTTTGCC 
CGATCATCCC CTAGAACTTC AATGAAAATA CAACAAAACC AGTCCATTCG GCGGCCCAGT 
CGACAGCTTG CTCCAAACAA GCCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515083.1
CDS	230..4039
Translation	MKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITLRKSVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQLQKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRISGLEEDLKTGKALLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQSQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMNLEKQNTELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDTTIASLEETNRTLTSDVANLANEKEELNNKLKDSQEQLSKLKDEEMSAAAIKAQFEKQLLNERTLKTQAVNKLAEIMNRKEPVKRGSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGMDSSSIGSGPGDAEPDDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	XM_006515020.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515020.4

ORF Insert Sequence:

ATGAAGCTTC TTAGTAAATT TGAAATGATA AAAAGATCAG ATTCTGCTTT TTTCTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAACAGT CCCTGGGTAG TTCAGCTCTT CTGTGCCTTT 
CAAGATGACA GGTACCTGTA CATGGTGATG GAGTACATGC CAGGCGGAGA CCTCGTCAAC 
CTTATGAGTA ATTATGATGT ACCTGAAAAG TGGGCCAAGT TTTACACTGC TGAAGTAGTG 
CTTGCACTGG ATGCAATACA CTCCATGGGC TTAATACACA GAGATGTGAA GCCTGATAAC 
ATGCTATTGG ATAAACATGG ACATCTAAAG TTAGCAGATT TTGGCACATG TATGAAAATG 
GATGAAACAG GCATGGTGCA TTGCGACACA GCAGTTGGAA CTCCCGACTA CATATCGCCC 
GAGGTTCTGA AATCACAAGG AGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTTTGA GATGTTAGTA GGGGACACTC CATTTTACGC AGATTCACTT 
GTAGGAACCT ACAGCAAAAT TATGGATCAT AAAAATTCAC TGTGTTTTCC TGAAGATACA 
GAAATTTCTA AACATGCGAA GAATCTCATA TGTGCCTTCT TAACAGACAG GGAGGTACGA 
CTTGGAAGAA ATGGGGTAGA AGAAATCAAA CAGCATCCTT TCTTTAAGAA TGATCAATGG 
AATTGGGATA ACATAAGAGA GACGGCAGCT CCAGTGGTAC CTGAGCTCAG CAGTGACATA 
GACAGCAGCA ACTTTGACGA CATTGAGGAT GACAAAGGCG ATGTAGAGAC CTTCCCGATT 
CCTAAAGCTT TTGTGGGAAA TCAGCTGCCT TTTATAGGAT TTACCTACTT TAGAGAAAAT 
TTGTTATTAA GTGACTCTCC ACCTTGTAGA GAAAATGATG CAATACAAAC AAGGAAAAGT 
GAAGAAAGTC AGGAGATTCA GAAAAAATTA TATGCACTAG AAGAACACCT TAGCAGTGAG 
GTGCAAGCCA AGGAGGAGCT GGAACAGAAG TGCAAATCTA TTAATACTCG CCTAGAGAAA 
ACAGCAAAGG AGCTAGAAGA AGAGATTACC TTACGGAAAA GTGTGGAATC AACATTAAGA 
CAGCTGGAAA GAGAAAAGGC CCTTCTTCAG CACAAAAATG CAGAGTATCA GCGGAAAGCT 
GATCATGAAG CCGACAAGAA GCGGAACTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATC TGAAGAAAAG GAACCAGAGC TCTCAGATAT CCACTGAGAA GGTCAATCAG 
CTCCAGAAAC AACTGGATGA AGCTAATGCT TTGCTGAGAA CAGAGTCTGA TACTGCAGCA 
CGGTTAAGAA AAACCCAGGC AGAAAGTTCC AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTT GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TTTAGAATCT GAAAGAAGGG ACCGGACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATCT GGGTTGGAAG AAGATTTGAA GACTGGCAAA 
GCCTTACTAG CAAAAGTAGA GCTGGAGAAG AGACAGCTGC AGGAGAAGCT CACTGACTTA 
GAGAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATATC AACTGAAAGT TATACAGCAG 
AGTTTAGAAC AAGAAGAAGC TGAACACAAG ACCACAAAAG CACGGCTAGC GGATAAAAAT 
AAGATCTATG AATCCATTGA AGAAGCTAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTGTTGGAAG AGAGGTCTTT AAAGCAGAAA GTGGAGAACC TGCTCCTGGA AGCCGAGAAA 
AGATGCTCTA TATTGGACTG TGACCTCAAA CAGTCTCAGC AGAAGCTAAA TGAGCTGCTG 
AAGCAGAAAG ATGTGCTGAA CGAGGATGTT AGAAATTTGA CATTAAAAAT AGAGCAGGAA 
ACTCAGAAGC GTTGTCTTAT GCAAAATGAC CTGAAGATGC AGACCCAACA AGTTAACACC 
CTAAAAATGT CAGAAAAGCA AATAAAACAA GAAAATAATC ATCTTATGGA AATGAAAATG 
AACTTGGAAA AGCAAAATAC TGAACTTCGA AAAGAGCGGC AGGATGCAGA TGGGCAGATG 
AAGGAACTGC AAGACCAACT TGAAGCAGAG CAGTATTTCT CAACCCTCTA TAAAACACAA 
GTTAGAGAAC TTAAAGAAGA AAATGAAGAG AAGACAAAAC TTTGTAAAGA GTTGCAACAG 
AAGAAGCAGG ATTTACAGGA TGAAAGAGAC TCTTTGGCTG CCCAGCTGGA AATTACCCTT 
ACCAAAGCAG ATTCTGAGCA GCTGGCTCGT TCCATTGCTG AAGAACAATA CTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAA 
GAACTTACTG AAAAGGATAC TACAATTGCA TCTCTTGAAG AAACAAATAG GACACTAACT 
AGCGATGTTG CAAATCTTGC AAATGAGAAA GAAGAATTAA ACAACAAGCT GAAAGATAGC 
CAAGAACAAC TGTCCAAGTT GAAAGATGAA GAGATGAGTG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTTCTGAA TGAGAGAACT CTCAAGACTC AAGCTGTGAA TAAGTTGGCG 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAGTG ACACAGATGT GCGAAGAAAA 
GAGAAGGAAA ACAGAAAGCT ACATATGGAG CTTAAGTCTG AACGTGAAAA ATTGACACAA 
CAGATGATCA AATATCAGAA AGAACTGAAT GAAATGCAGG CTCAAATAGC TGAAGAGAGC 
CAAATTCGAA TAGAACTCCA GATGACCCTG GACAGTAAAG ACAGCGACAT TGAACAGCTG 
CGGTCACAGC TCCAGGCCTT GCATATTGGT ATGGACAGTT CCAGTATAGG CAGTGGACCA 
GGCGATGCTG AGCCTGATGA TGGATTTCCA GAATCAAGAT TAGAAGGATG GTTGTCATTG 
CCTGTGCGCA ACAACACTAA AAAGTTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGC 
AAGAAGATTC TTTTTTATGA CAGCGAACAA GATAAAGAGC AGTCCAACCC TTACATGGTG 
TTAGACATAG ACAAGCTATT TCACGTCCGA CCTGTTACCC AGACAGATGT ATACAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTTCAG ATTCTATATG CCAATGAAGG AGAAAGTAAG 
AAGGAACCAG AGTTTCCAGT GGAACCAGTG GGAGAAAAAT CTAATTATAT TTGTCACAAA 
GGACATGAGT TTATTCCTAC TCTTTATCAC TTCCCAACCA ACTGTGAGGC ATGTATGAAG 
CCGCTGTGGC ACATGTTTAA GCCTCCTCCT GCTCTAGAGT GCCGTAGGTG CCACATTAAA 
TGTCATAAAG ATCACATGGA CAAGAAGGAA GAGATTATAG CGCCCTGCAA AGTTTATTAT 
GATATATCGT CGGCAAAGAA TCTGTTGTTA TTAGCAAATT CAACAGAAGA ACAGCAGAAA 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCTC CAGCTCCAGA CCCTTTTGCC 
CGATCATCCC CTAGAACTTC AATGAAAATA CAACAAAACC AGTCCATTCG GCGGCCCAGT 
CGACAGCTTG CTCCAAACAA GCCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rock2 ( XM_006515019.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515082.1 Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47266
Clone ID Related Accession (Same CDS sequence)	XM_006515019.4 , XM_006515020.4 , XM_006515019.3 , XM_006515020.3
Accession Version	XM_006515019.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515019.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTAGTAAATT TGAAATGATA AAAAGATCAG ATTCTGCTTT TTTCTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAACAGT CCCTGGGTAG TTCAGCTCTT CTGTGCCTTT 
CAAGATGACA GGTACCTGTA CATGGTGATG GAGTACATGC CAGGCGGAGA CCTCGTCAAC 
CTTATGAGTA ATTATGATGT ACCTGAAAAG TGGGCCAAGT TTTACACTGC TGAAGTAGTG 
CTTGCACTGG ATGCAATACA CTCCATGGGC TTAATACACA GAGATGTGAA GCCTGATAAC 
ATGCTATTGG ATAAACATGG ACATCTAAAG TTAGCAGATT TTGGCACATG TATGAAAATG 
GATGAAACAG GCATGGTGCA TTGCGACACA GCAGTTGGAA CTCCCGACTA CATATCGCCC 
GAGGTTCTGA AATCACAAGG AGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTTTGA GATGTTAGTA GGGGACACTC CATTTTACGC AGATTCACTT 
GTAGGAACCT ACAGCAAAAT TATGGATCAT AAAAATTCAC TGTGTTTTCC TGAAGATACA 
GAAATTTCTA AACATGCGAA GAATCTCATA TGTGCCTTCT TAACAGACAG GGAGGTACGA 
CTTGGAAGAA ATGGGGTAGA AGAAATCAAA CAGCATCCTT TCTTTAAGAA TGATCAATGG 
AATTGGGATA ACATAAGAGA GACGGCAGCT CCAGTGGTAC CTGAGCTCAG CAGTGACATA 
GACAGCAGCA ACTTTGACGA CATTGAGGAT GACAAAGGCG ATGTAGAGAC CTTCCCGATT 
CCTAAAGCTT TTGTGGGAAA TCAGCTGCCT TTTATAGGAT TTACCTACTT TAGAGAAAAT 
TTGTTATTAA GTGACTCTCC ACCTTGTAGA GAAAATGATG CAATACAAAC AAGGAAAAGT 
GAAGAAAGTC AGGAGATTCA GAAAAAATTA TATGCACTAG AAGAACACCT TAGCAGTGAG 
GTGCAAGCCA AGGAGGAGCT GGAACAGAAG TGCAAATCTA TTAATACTCG CCTAGAGAAA 
ACAGCAAAGG AGCTAGAAGA AGAGATTACC TTACGGAAAA GTGTGGAATC AACATTAAGA 
CAGCTGGAAA GAGAAAAGGC CCTTCTTCAG CACAAAAATG CAGAGTATCA GCGGAAAGCT 
GATCATGAAG CCGACAAGAA GCGGAACTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATC TGAAGAAAAG GAACCAGAGC TCTCAGATAT CCACTGAGAA GGTCAATCAG 
CTCCAGAAAC AACTGGATGA AGCTAATGCT TTGCTGAGAA CAGAGTCTGA TACTGCAGCA 
CGGTTAAGAA AAACCCAGGC AGAAAGTTCC AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTT GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TTTAGAATCT GAAAGAAGGG ACCGGACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATCT GGGTTGGAAG AAGATTTGAA GACTGGCAAA 
GCCTTACTAG CAAAAGTAGA GCTGGAGAAG AGACAGCTGC AGGAGAAGCT CACTGACTTA 
GAGAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATATC AACTGAAAGT TATACAGCAG 
AGTTTAGAAC AAGAAGAAGC TGAACACAAG ACCACAAAAG CACGGCTAGC GGATAAAAAT 
AAGATCTATG AATCCATTGA AGAAGCTAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTGTTGGAAG AGAGGTCTTT AAAGCAGAAA GTGGAGAACC TGCTCCTGGA AGCCGAGAAA 
AGATGCTCTA TATTGGACTG TGACCTCAAA CAGTCTCAGC AGAAGCTAAA TGAGCTGCTG 
AAGCAGAAAG ATGTGCTGAA CGAGGATGTT AGAAATTTGA CATTAAAAAT AGAGCAGGAA 
ACTCAGAAGC GTTGTCTTAT GCAAAATGAC CTGAAGATGC AGACCCAACA AGTTAACACC 
CTAAAAATGT CAGAAAAGCA AATAAAACAA GAAAATAATC ATCTTATGGA AATGAAAATG 
AACTTGGAAA AGCAAAATAC TGAACTTCGA AAAGAGCGGC AGGATGCAGA TGGGCAGATG 
AAGGAACTGC AAGACCAACT TGAAGCAGAG CAGTATTTCT CAACCCTCTA TAAAACACAA 
GTTAGAGAAC TTAAAGAAGA AAATGAAGAG AAGACAAAAC TTTGTAAAGA GTTGCAACAG 
AAGAAGCAGG ATTTACAGGA TGAAAGAGAC TCTTTGGCTG CCCAGCTGGA AATTACCCTT 
ACCAAAGCAG ATTCTGAGCA GCTGGCTCGT TCCATTGCTG AAGAACAATA CTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAA 
GAACTTACTG AAAAGGATAC TACAATTGCA TCTCTTGAAG AAACAAATAG GACACTAACT 
AGCGATGTTG CAAATCTTGC AAATGAGAAA GAAGAATTAA ACAACAAGCT GAAAGATAGC 
CAAGAACAAC TGTCCAAGTT GAAAGATGAA GAGATGAGTG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTTCTGAA TGAGAGAACT CTCAAGACTC AAGCTGTGAA TAAGTTGGCG 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAGTG ACACAGATGT GCGAAGAAAA 
GAGAAGGAAA ACAGAAAGCT ACATATGGAG CTTAAGTCTG AACGTGAAAA ATTGACACAA 
CAGATGATCA AATATCAGAA AGAACTGAAT GAAATGCAGG CTCAAATAGC TGAAGAGAGC 
CAAATTCGAA TAGAACTCCA GATGACCCTG GACAGTAAAG ACAGCGACAT TGAACAGCTG 
CGGTCACAGC TCCAGGCCTT GCATATTGGT ATGGACAGTT CCAGTATAGG CAGTGGACCA 
GGCGATGCTG AGCCTGATGA TGGATTTCCA GAATCAAGAT TAGAAGGATG GTTGTCATTG 
CCTGTGCGCA ACAACACTAA AAAGTTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGC 
AAGAAGATTC TTTTTTATGA CAGCGAACAA GATAAAGAGC AGTCCAACCC TTACATGGTG 
TTAGACATAG ACAAGCTATT TCACGTCCGA CCTGTTACCC AGACAGATGT ATACAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTTCAG ATTCTATATG CCAATGAAGG AGAAAGTAAG 
AAGGAACCAG AGTTTCCAGT GGAACCAGTG GGAGAAAAAT CTAATTATAT TTGTCACAAA 
GGACATGAGT TTATTCCTAC TCTTTATCAC TTCCCAACCA ACTGTGAGGC ATGTATGAAG 
CCGCTGTGGC ACATGTTTAA GCCTCCTCCT GCTCTAGAGT GCCGTAGGTG CCACATTAAA 
TGTCATAAAG ATCACATGGA CAAGAAGGAA GAGATTATAG CGCCCTGCAA AGTTTATTAT 
GATATATCGT CGGCAAAGAA TCTGTTGTTA TTAGCAAATT CAACAGAAGA ACAGCAGAAA 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCTC CAGCTCCAGA CCCTTTTGCC 
CGATCATCCC CTAGAACTTC AATGAAAATA CAACAAAACC AGTCCATTCG GCGGCCCAGT 
CGACAGCTTG CTCCAAACAA GCCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515082.1
CDS	398..4207
Translation	MKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITLRKSVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQLQKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRISGLEEDLKTGKALLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQSQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMNLEKQNTELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDTTIASLEETNRTLTSDVANLANEKEELNNKLKDSQEQLSKLKDEEMSAAAIKAQFEKQLLNERTLKTQAVNKLAEIMNRKEPVKRGSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGMDSSSIGSGPGDAEPDDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	XM_006515019.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515019.3

ORF Insert Sequence:

ATGAAGCTTC TTAGTAAATT TGAAATGATA AAAAGATCAG ATTCTGCTTT TTTCTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAACAGT CCCTGGGTAG TTCAGCTCTT CTGTGCCTTT 
CAAGATGACA GGTACCTGTA CATGGTGATG GAGTACATGC CAGGCGGAGA CCTCGTCAAC 
CTTATGAGTA ATTATGATGT ACCTGAAAAG TGGGCCAAGT TTTACACTGC TGAAGTAGTG 
CTTGCACTGG ATGCAATACA CTCCATGGGC TTAATACACA GAGATGTGAA GCCTGATAAC 
ATGCTATTGG ATAAACATGG ACATCTAAAG TTAGCAGATT TTGGCACATG TATGAAAATG 
GATGAAACAG GCATGGTGCA TTGCGACACA GCAGTTGGAA CTCCCGACTA CATATCGCCC 
GAGGTTCTGA AATCACAAGG AGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTTTGA GATGTTAGTA GGGGACACTC CATTTTACGC AGATTCACTT 
GTAGGAACCT ACAGCAAAAT TATGGATCAT AAAAATTCAC TGTGTTTTCC TGAAGATACA 
GAAATTTCTA AACATGCGAA GAATCTCATA TGTGCCTTCT TAACAGACAG GGAGGTACGA 
CTTGGAAGAA ATGGGGTAGA AGAAATCAAA CAGCATCCTT TCTTTAAGAA TGATCAATGG 
AATTGGGATA ACATAAGAGA GACGGCAGCT CCAGTGGTAC CTGAGCTCAG CAGTGACATA 
GACAGCAGCA ACTTTGACGA CATTGAGGAT GACAAAGGCG ATGTAGAGAC CTTCCCGATT 
CCTAAAGCTT TTGTGGGAAA TCAGCTGCCT TTTATAGGAT TTACCTACTT TAGAGAAAAT 
TTGTTATTAA GTGACTCTCC ACCTTGTAGA GAAAATGATG CAATACAAAC AAGGAAAAGT 
GAAGAAAGTC AGGAGATTCA GAAAAAATTA TATGCACTAG AAGAACACCT TAGCAGTGAG 
GTGCAAGCCA AGGAGGAGCT GGAACAGAAG TGCAAATCTA TTAATACTCG CCTAGAGAAA 
ACAGCAAAGG AGCTAGAAGA AGAGATTACC TTACGGAAAA GTGTGGAATC AACATTAAGA 
CAGCTGGAAA GAGAAAAGGC CCTTCTTCAG CACAAAAATG CAGAGTATCA GCGGAAAGCT 
GATCATGAAG CCGACAAGAA GCGGAACTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATC TGAAGAAAAG GAACCAGAGC TCTCAGATAT CCACTGAGAA GGTCAATCAG 
CTCCAGAAAC AACTGGATGA AGCTAATGCT TTGCTGAGAA CAGAGTCTGA TACTGCAGCA 
CGGTTAAGAA AAACCCAGGC AGAAAGTTCC AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTT GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TTTAGAATCT GAAAGAAGGG ACCGGACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATCT GGGTTGGAAG AAGATTTGAA GACTGGCAAA 
GCCTTACTAG CAAAAGTAGA GCTGGAGAAG AGACAGCTGC AGGAGAAGCT CACTGACTTA 
GAGAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATATC AACTGAAAGT TATACAGCAG 
AGTTTAGAAC AAGAAGAAGC TGAACACAAG ACCACAAAAG CACGGCTAGC GGATAAAAAT 
AAGATCTATG AATCCATTGA AGAAGCTAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTGTTGGAAG AGAGGTCTTT AAAGCAGAAA GTGGAGAACC TGCTCCTGGA AGCCGAGAAA 
AGATGCTCTA TATTGGACTG TGACCTCAAA CAGTCTCAGC AGAAGCTAAA TGAGCTGCTG 
AAGCAGAAAG ATGTGCTGAA CGAGGATGTT AGAAATTTGA CATTAAAAAT AGAGCAGGAA 
ACTCAGAAGC GTTGTCTTAT GCAAAATGAC CTGAAGATGC AGACCCAACA AGTTAACACC 
CTAAAAATGT CAGAAAAGCA AATAAAACAA GAAAATAATC ATCTTATGGA AATGAAAATG 
AACTTGGAAA AGCAAAATAC TGAACTTCGA AAAGAGCGGC AGGATGCAGA TGGGCAGATG 
AAGGAACTGC AAGACCAACT TGAAGCAGAG CAGTATTTCT CAACCCTCTA TAAAACACAA 
GTTAGAGAAC TTAAAGAAGA AAATGAAGAG AAGACAAAAC TTTGTAAAGA GTTGCAACAG 
AAGAAGCAGG ATTTACAGGA TGAAAGAGAC TCTTTGGCTG CCCAGCTGGA AATTACCCTT 
ACCAAAGCAG ATTCTGAGCA GCTGGCTCGT TCCATTGCTG AAGAACAATA CTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAA 
GAACTTACTG AAAAGGATAC TACAATTGCA TCTCTTGAAG AAACAAATAG GACACTAACT 
AGCGATGTTG CAAATCTTGC AAATGAGAAA GAAGAATTAA ACAACAAGCT GAAAGATAGC 
CAAGAACAAC TGTCCAAGTT GAAAGATGAA GAGATGAGTG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTTCTGAA TGAGAGAACT CTCAAGACTC AAGCTGTGAA TAAGTTGGCG 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAGTG ACACAGATGT GCGAAGAAAA 
GAGAAGGAAA ACAGAAAGCT ACATATGGAG CTTAAGTCTG AACGTGAAAA ATTGACACAA 
CAGATGATCA AATATCAGAA AGAACTGAAT GAAATGCAGG CTCAAATAGC TGAAGAGAGC 
CAAATTCGAA TAGAACTCCA GATGACCCTG GACAGTAAAG ACAGCGACAT TGAACAGCTG 
CGGTCACAGC TCCAGGCCTT GCATATTGGT ATGGACAGTT CCAGTATAGG CAGTGGACCA 
GGCGATGCTG AGCCTGATGA TGGATTTCCA GAATCAAGAT TAGAAGGATG GTTGTCATTG 
CCTGTGCGCA ACAACACTAA AAAGTTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGC 
AAGAAGATTC TTTTTTATGA CAGCGAACAA GATAAAGAGC AGTCCAACCC TTACATGGTG 
TTAGACATAG ACAAGCTATT TCACGTCCGA CCTGTTACCC AGACAGATGT ATACAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTTCAG ATTCTATATG CCAATGAAGG AGAAAGTAAG 
AAGGAACCAG AGTTTCCAGT GGAACCAGTG GGAGAAAAAT CTAATTATAT TTGTCACAAA 
GGACATGAGT TTATTCCTAC TCTTTATCAC TTCCCAACCA ACTGTGAGGC ATGTATGAAG 
CCGCTGTGGC ACATGTTTAA GCCTCCTCCT GCTCTAGAGT GCCGTAGGTG CCACATTAAA 
TGTCATAAAG ATCACATGGA CAAGAAGGAA GAGATTATAG CGCCCTGCAA AGTTTATTAT 
GATATATCGT CGGCAAAGAA TCTGTTGTTA TTAGCAAATT CAACAGAAGA ACAGCAGAAA 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCTC CAGCTCCAGA CCCTTTTGCC 
CGATCATCCC CTAGAACTTC AATGAAAATA CAACAAAACC AGTCCATTCG GCGGCCCAGT 
CGACAGCTTG CTCCAAACAA GCCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rock2 ( XM_006515020.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515083.1 Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu47266
Clone ID Related Accession (Same CDS sequence)	XM_006515019.4 , XM_006515020.4 , XM_006515019.3 , XM_006515020.3
Accession Version	XM_006515020.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515020.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTC TTAGTAAATT TGAAATGATA AAAAGATCAG ATTCTGCTTT TTTCTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAACAGT CCCTGGGTAG TTCAGCTCTT CTGTGCCTTT 
CAAGATGACA GGTACCTGTA CATGGTGATG GAGTACATGC CAGGCGGAGA CCTCGTCAAC 
CTTATGAGTA ATTATGATGT ACCTGAAAAG TGGGCCAAGT TTTACACTGC TGAAGTAGTG 
CTTGCACTGG ATGCAATACA CTCCATGGGC TTAATACACA GAGATGTGAA GCCTGATAAC 
ATGCTATTGG ATAAACATGG ACATCTAAAG TTAGCAGATT TTGGCACATG TATGAAAATG 
GATGAAACAG GCATGGTGCA TTGCGACACA GCAGTTGGAA CTCCCGACTA CATATCGCCC 
GAGGTTCTGA AATCACAAGG AGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTTTGA GATGTTAGTA GGGGACACTC CATTTTACGC AGATTCACTT 
GTAGGAACCT ACAGCAAAAT TATGGATCAT AAAAATTCAC TGTGTTTTCC TGAAGATACA 
GAAATTTCTA AACATGCGAA GAATCTCATA TGTGCCTTCT TAACAGACAG GGAGGTACGA 
CTTGGAAGAA ATGGGGTAGA AGAAATCAAA CAGCATCCTT TCTTTAAGAA TGATCAATGG 
AATTGGGATA ACATAAGAGA GACGGCAGCT CCAGTGGTAC CTGAGCTCAG CAGTGACATA 
GACAGCAGCA ACTTTGACGA CATTGAGGAT GACAAAGGCG ATGTAGAGAC CTTCCCGATT 
CCTAAAGCTT TTGTGGGAAA TCAGCTGCCT TTTATAGGAT TTACCTACTT TAGAGAAAAT 
TTGTTATTAA GTGACTCTCC ACCTTGTAGA GAAAATGATG CAATACAAAC AAGGAAAAGT 
GAAGAAAGTC AGGAGATTCA GAAAAAATTA TATGCACTAG AAGAACACCT TAGCAGTGAG 
GTGCAAGCCA AGGAGGAGCT GGAACAGAAG TGCAAATCTA TTAATACTCG CCTAGAGAAA 
ACAGCAAAGG AGCTAGAAGA AGAGATTACC TTACGGAAAA GTGTGGAATC AACATTAAGA 
CAGCTGGAAA GAGAAAAGGC CCTTCTTCAG CACAAAAATG CAGAGTATCA GCGGAAAGCT 
GATCATGAAG CCGACAAGAA GCGGAACTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATC TGAAGAAAAG GAACCAGAGC TCTCAGATAT CCACTGAGAA GGTCAATCAG 
CTCCAGAAAC AACTGGATGA AGCTAATGCT TTGCTGAGAA CAGAGTCTGA TACTGCAGCA 
CGGTTAAGAA AAACCCAGGC AGAAAGTTCC AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTT GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TTTAGAATCT GAAAGAAGGG ACCGGACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATCT GGGTTGGAAG AAGATTTGAA GACTGGCAAA 
GCCTTACTAG CAAAAGTAGA GCTGGAGAAG AGACAGCTGC AGGAGAAGCT CACTGACTTA 
GAGAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATATC AACTGAAAGT TATACAGCAG 
AGTTTAGAAC AAGAAGAAGC TGAACACAAG ACCACAAAAG CACGGCTAGC GGATAAAAAT 
AAGATCTATG AATCCATTGA AGAAGCTAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTGTTGGAAG AGAGGTCTTT AAAGCAGAAA GTGGAGAACC TGCTCCTGGA AGCCGAGAAA 
AGATGCTCTA TATTGGACTG TGACCTCAAA CAGTCTCAGC AGAAGCTAAA TGAGCTGCTG 
AAGCAGAAAG ATGTGCTGAA CGAGGATGTT AGAAATTTGA CATTAAAAAT AGAGCAGGAA 
ACTCAGAAGC GTTGTCTTAT GCAAAATGAC CTGAAGATGC AGACCCAACA AGTTAACACC 
CTAAAAATGT CAGAAAAGCA AATAAAACAA GAAAATAATC ATCTTATGGA AATGAAAATG 
AACTTGGAAA AGCAAAATAC TGAACTTCGA AAAGAGCGGC AGGATGCAGA TGGGCAGATG 
AAGGAACTGC AAGACCAACT TGAAGCAGAG CAGTATTTCT CAACCCTCTA TAAAACACAA 
GTTAGAGAAC TTAAAGAAGA AAATGAAGAG AAGACAAAAC TTTGTAAAGA GTTGCAACAG 
AAGAAGCAGG ATTTACAGGA TGAAAGAGAC TCTTTGGCTG CCCAGCTGGA AATTACCCTT 
ACCAAAGCAG ATTCTGAGCA GCTGGCTCGT TCCATTGCTG AAGAACAATA CTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAA 
GAACTTACTG AAAAGGATAC TACAATTGCA TCTCTTGAAG AAACAAATAG GACACTAACT 
AGCGATGTTG CAAATCTTGC AAATGAGAAA GAAGAATTAA ACAACAAGCT GAAAGATAGC 
CAAGAACAAC TGTCCAAGTT GAAAGATGAA GAGATGAGTG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTTCTGAA TGAGAGAACT CTCAAGACTC AAGCTGTGAA TAAGTTGGCG 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAGTG ACACAGATGT GCGAAGAAAA 
GAGAAGGAAA ACAGAAAGCT ACATATGGAG CTTAAGTCTG AACGTGAAAA ATTGACACAA 
CAGATGATCA AATATCAGAA AGAACTGAAT GAAATGCAGG CTCAAATAGC TGAAGAGAGC 
CAAATTCGAA TAGAACTCCA GATGACCCTG GACAGTAAAG ACAGCGACAT TGAACAGCTG 
CGGTCACAGC TCCAGGCCTT GCATATTGGT ATGGACAGTT CCAGTATAGG CAGTGGACCA 
GGCGATGCTG AGCCTGATGA TGGATTTCCA GAATCAAGAT TAGAAGGATG GTTGTCATTG 
CCTGTGCGCA ACAACACTAA AAAGTTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGC 
AAGAAGATTC TTTTTTATGA CAGCGAACAA GATAAAGAGC AGTCCAACCC TTACATGGTG 
TTAGACATAG ACAAGCTATT TCACGTCCGA CCTGTTACCC AGACAGATGT ATACAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTTCAG ATTCTATATG CCAATGAAGG AGAAAGTAAG 
AAGGAACCAG AGTTTCCAGT GGAACCAGTG GGAGAAAAAT CTAATTATAT TTGTCACAAA 
GGACATGAGT TTATTCCTAC TCTTTATCAC TTCCCAACCA ACTGTGAGGC ATGTATGAAG 
CCGCTGTGGC ACATGTTTAA GCCTCCTCCT GCTCTAGAGT GCCGTAGGTG CCACATTAAA 
TGTCATAAAG ATCACATGGA CAAGAAGGAA GAGATTATAG CGCCCTGCAA AGTTTATTAT 
GATATATCGT CGGCAAAGAA TCTGTTGTTA TTAGCAAATT CAACAGAAGA ACAGCAGAAA 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCTC CAGCTCCAGA CCCTTTTGCC 
CGATCATCCC CTAGAACTTC AATGAAAATA CAACAAAACC AGTCCATTCG GCGGCCCAGT 
CGACAGCTTG CTCCAAACAA GCCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515083.1
CDS	230..4039
Translation	MKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENLLLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITLRKSVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQLQKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESERRDRTHGSEIINDLQGRISGLEEDLKTGKALLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQSLEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQSQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMNLEKQNTELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDTTIASLEETNRTLTSDVANLANEKEELNNKLKDSQEQLSKLKDEEMSAAAIKAQFEKQLLNERTLKTQAVNKLAEIMNRKEPVKRGSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGMDSSSIGSGPGDAEPDDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	XM_006515020.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho-associated coiled-coil containing protein kinase 2 (Rock2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515020.3

ORF Insert Sequence:

ATGAAGCTTC TTAGTAAATT TGAAATGATA AAAAGATCAG ATTCTGCTTT TTTCTGGGAA 
GAAAGAGATA TTATGGCCTT TGCCAACAGT CCCTGGGTAG TTCAGCTCTT CTGTGCCTTT 
CAAGATGACA GGTACCTGTA CATGGTGATG GAGTACATGC CAGGCGGAGA CCTCGTCAAC 
CTTATGAGTA ATTATGATGT ACCTGAAAAG TGGGCCAAGT TTTACACTGC TGAAGTAGTG 
CTTGCACTGG ATGCAATACA CTCCATGGGC TTAATACACA GAGATGTGAA GCCTGATAAC 
ATGCTATTGG ATAAACATGG ACATCTAAAG TTAGCAGATT TTGGCACATG TATGAAAATG 
GATGAAACAG GCATGGTGCA TTGCGACACA GCAGTTGGAA CTCCCGACTA CATATCGCCC 
GAGGTTCTGA AATCACAAGG AGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCT 
GTAGGTGTTT TCCTTTTTGA GATGTTAGTA GGGGACACTC CATTTTACGC AGATTCACTT 
GTAGGAACCT ACAGCAAAAT TATGGATCAT AAAAATTCAC TGTGTTTTCC TGAAGATACA 
GAAATTTCTA AACATGCGAA GAATCTCATA TGTGCCTTCT TAACAGACAG GGAGGTACGA 
CTTGGAAGAA ATGGGGTAGA AGAAATCAAA CAGCATCCTT TCTTTAAGAA TGATCAATGG 
AATTGGGATA ACATAAGAGA GACGGCAGCT CCAGTGGTAC CTGAGCTCAG CAGTGACATA 
GACAGCAGCA ACTTTGACGA CATTGAGGAT GACAAAGGCG ATGTAGAGAC CTTCCCGATT 
CCTAAAGCTT TTGTGGGAAA TCAGCTGCCT TTTATAGGAT TTACCTACTT TAGAGAAAAT 
TTGTTATTAA GTGACTCTCC ACCTTGTAGA GAAAATGATG CAATACAAAC AAGGAAAAGT 
GAAGAAAGTC AGGAGATTCA GAAAAAATTA TATGCACTAG AAGAACACCT TAGCAGTGAG 
GTGCAAGCCA AGGAGGAGCT GGAACAGAAG TGCAAATCTA TTAATACTCG CCTAGAGAAA 
ACAGCAAAGG AGCTAGAAGA AGAGATTACC TTACGGAAAA GTGTGGAATC AACATTAAGA 
CAGCTGGAAA GAGAAAAGGC CCTTCTTCAG CACAAAAATG CAGAGTATCA GCGGAAAGCT 
GATCATGAAG CCGACAAGAA GCGGAACTTG GAAAATGATG TTAACAGCTT AAAAGATCAA 
CTTGAAGATC TGAAGAAAAG GAACCAGAGC TCTCAGATAT CCACTGAGAA GGTCAATCAG 
CTCCAGAAAC AACTGGATGA AGCTAATGCT TTGCTGAGAA CAGAGTCTGA TACTGCAGCA 
CGGTTAAGAA AAACCCAGGC AGAAAGTTCC AAACAGATTC AGCAGCTGGA ATCTAACAAT 
AGAGATCTAC AAGATAAAAA CTGCCTGCTT GAGACTGCCA AGTTAAAACT TGAAAAGGAA 
TTTATCAATC TTCAGTCAGC TTTAGAATCT GAAAGAAGGG ACCGGACCCA TGGATCAGAG 
ATAATTAATG ATTTACAAGG TAGAATATCT GGGTTGGAAG AAGATTTGAA GACTGGCAAA 
GCCTTACTAG CAAAAGTAGA GCTGGAGAAG AGACAGCTGC AGGAGAAGCT CACTGACTTA 
GAGAAGGAAA AAAGCAACAT GGAAATAGAT ATGACATATC AACTGAAAGT TATACAGCAG 
AGTTTAGAAC AAGAAGAAGC TGAACACAAG ACCACAAAAG CACGGCTAGC GGATAAAAAT 
AAGATCTATG AATCCATTGA AGAAGCTAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG 
CTGTTGGAAG AGAGGTCTTT AAAGCAGAAA GTGGAGAACC TGCTCCTGGA AGCCGAGAAA 
AGATGCTCTA TATTGGACTG TGACCTCAAA CAGTCTCAGC AGAAGCTAAA TGAGCTGCTG 
AAGCAGAAAG ATGTGCTGAA CGAGGATGTT AGAAATTTGA CATTAAAAAT AGAGCAGGAA 
ACTCAGAAGC GTTGTCTTAT GCAAAATGAC CTGAAGATGC AGACCCAACA AGTTAACACC 
CTAAAAATGT CAGAAAAGCA AATAAAACAA GAAAATAATC ATCTTATGGA AATGAAAATG 
AACTTGGAAA AGCAAAATAC TGAACTTCGA AAAGAGCGGC AGGATGCAGA TGGGCAGATG 
AAGGAACTGC AAGACCAACT TGAAGCAGAG CAGTATTTCT CAACCCTCTA TAAAACACAA 
GTTAGAGAAC TTAAAGAAGA AAATGAAGAG AAGACAAAAC TTTGTAAAGA GTTGCAACAG 
AAGAAGCAGG ATTTACAGGA TGAAAGAGAC TCTTTGGCTG CCCAGCTGGA AATTACCCTT 
ACCAAAGCAG ATTCTGAGCA GCTGGCTCGT TCCATTGCTG AAGAACAATA CTCTGATTTG 
GAAAAAGAGA AGATCATGAA AGAGCTGGAG ATCAAAGAGA TGATGGCTAG ACACAAACAA 
GAACTTACTG AAAAGGATAC TACAATTGCA TCTCTTGAAG AAACAAATAG GACACTAACT 
AGCGATGTTG CAAATCTTGC AAATGAGAAA GAAGAATTAA ACAACAAGCT GAAAGATAGC 
CAAGAACAAC TGTCCAAGTT GAAAGATGAA GAGATGAGTG CAGCAGCTAT TAAAGCACAG 
TTTGAGAAGC AGCTTCTGAA TGAGAGAACT CTCAAGACTC AAGCTGTGAA TAAGTTGGCG 
GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAGTG ACACAGATGT GCGAAGAAAA 
GAGAAGGAAA ACAGAAAGCT ACATATGGAG CTTAAGTCTG AACGTGAAAA ATTGACACAA 
CAGATGATCA AATATCAGAA AGAACTGAAT GAAATGCAGG CTCAAATAGC TGAAGAGAGC 
CAAATTCGAA TAGAACTCCA GATGACCCTG GACAGTAAAG ACAGCGACAT TGAACAGCTG 
CGGTCACAGC TCCAGGCCTT GCATATTGGT ATGGACAGTT CCAGTATAGG CAGTGGACCA 
GGCGATGCTG AGCCTGATGA TGGATTTCCA GAATCAAGAT TAGAAGGATG GTTGTCATTG 
CCTGTGCGCA ACAACACTAA AAAGTTTGGA TGGGTTAAAA AGTATGTGAT TGTAAGCAGC 
AAGAAGATTC TTTTTTATGA CAGCGAACAA GATAAAGAGC AGTCCAACCC TTACATGGTG 
TTAGACATAG ACAAGCTATT TCACGTCCGA CCTGTTACCC AGACAGATGT ATACAGAGCA 
GATGCTAAAG AAATTCCAAG GATATTTCAG ATTCTATATG CCAATGAAGG AGAAAGTAAG 
AAGGAACCAG AGTTTCCAGT GGAACCAGTG GGAGAAAAAT CTAATTATAT TTGTCACAAA 
GGACATGAGT TTATTCCTAC TCTTTATCAC TTCCCAACCA ACTGTGAGGC ATGTATGAAG 
CCGCTGTGGC ACATGTTTAA GCCTCCTCCT GCTCTAGAGT GCCGTAGGTG CCACATTAAA 
TGTCATAAAG ATCACATGGA CAAGAAGGAA GAGATTATAG CGCCCTGCAA AGTTTATTAT 
GATATATCGT CGGCAAAGAA TCTGTTGTTA TTAGCAAATT CAACAGAAGA ACAGCAGAAA 
TGGGTTAGTC GGTTGGTGAA AAAGATACCT AAAAAGCCTC CAGCTCCAGA CCCTTTTGCC 
CGATCATCCC CTAGAACTTC AATGAAAATA CAACAAAACC AGTCCATTCG GCGGCCCAGT 
CGACAGCTTG CTCCAAACAA GCCAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fasudil or genetic depletion of ROCK1 or ROCK2 induces anxiety-like behaviors.
Behavioural brain research373112083(2019 Nov)
Greathouse KM,Henderson BW,Gentry EG,Herskowitz JH

ROCK2 promotes ryanodine receptor phosphorylation and arrhythmic calcium release in diabetic cardiomyocytes.
International journal of cardiology28190-98(2019 Apr)
Soliman H,Nyamandi V,Garcia-Patino M,Zhang PC,Lin E,Jia ZP,Tibbits GF,Hove-Madsen L,MacLeod KM

Distinct and complementary functions of rho kinase isoforms ROCK1 and ROCK2 in prefrontal cortex structural plasticity.
Brain structure & function223(9)4227-4241(2018 Dec)
Greathouse KM,Boros BD,Deslauriers JF,Henderson BW,Curtis KA,Gentry EG,Herskowitz JH

Different roles of myocardial ROCK1 and ROCK2 in cardiac dysfunction and postcapillary pulmonary hypertension in mice.
Proceedings of the National Academy of Sciences of the United States of America115(30)E7129-E7138(2018 07)
Sunamura S,Satoh K,Kurosawa R,Ohtsuki T,Kikuchi N,Elias-Al-Mamun M,Shimizu T,Ikeda S,Suzuki K,Satoh T,Omura J,Nogi M,Numano K,Siddique MAH,Miyata S,Miura M,Shimokawa H

Toll-like receptor signaling in macrophages is regulated by extracellular substrate stiffness and Rho-associated coiled-coil kinase (ROCK1/2).
International immunology30(6)267-278(2018 05)
Gruber E,Heyward C,Cameron J,Leifer C

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Rock2 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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