Siah2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Siah2
Entrez Gene ID	140593
Full Name	siah E3 ubiquitin protein ligase 2
General protein information	Preferred Names siah E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase SIAH2 RING-type E3 ubiquitin transferase SIAH2 seven in absentia 2 seven in absentia homolog 2 siah-2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q26
Summary	a synaptophysin binding protein and also interact with E2 ubiquitin-conjugating enzyme UbcH8; involved in the regulating the ubiquitination and degradation of synaptophysin [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_134457.1	NP_604452.1	E3 ubiquitin-protein ligase SIAH2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SIAH2	XP_516819.2
Macaca mulatta (Rhesus monkey)	SIAH2	XP_001107642.1
Mus musculus (house mouse)	Siah2	NP_033200.2
Rattus norvegicus (Norway rat)	Siah2	NP_604452.1
Gallus gallus (chicken)	SIAH2	XP_426719.2
Danio rerio (zebrafish)	siah2l	NP_956721.1
Canis lupus familiaris (dog)	SIAH2	XP_005634711.1
Bos taurus (cattle)	SIAH2	NP_001193983.1
Homo sapiens (human)	SIAH2	NP_005058.3
Xenopus tropicalis (tropical clawed frog)	siah2	NP_001095281.1

Gene Ontology
Bibliography

Related articles in PubMed

Ubiquitin ligase Siah2 regulates RevErbα degradation and the mammalian circadian clock.
DeBruyne JP, Baggs JE, Sato TK, Hogenesch JB
Proceedings of the National Academy of Sciences of the United States of America112(40)12420-5(2015 Oct)

Exchange Factor TBL1 and Arginine Methyltransferase PRMT6 Cooperate in Protecting G Protein Pathway Suppressor 2 (GPS2) from Proteasomal Degradation.
Huang J, Cardamone MD, Johnson HE, Neault M, Chan M, Floyd ZE, Mallette FA, Perissi V
The Journal of biological chemistry290(31)19044-54(2015 Jul)

The Nrf2 regulatory network provides an interface between redox and intermediary metabolism.
Hayes JD, Dinkova-Kostova AT
Trends in biochemical sciences39(4)199-218(2014 Apr)

Possible role of death receptor-mediated apoptosis by the E3 ubiquitin ligases Siah2 and POSH.
Christian PA, Fiandalo MV, Schwarze SR
Molecular cancer1057(2011 May)

Maturation of human dendritic cells is accompanied by functional remodelling of the ubiquitin-proteasome system.
Ebstein F, Lange N, Urban S, Seifert U, Krüger E, Kloetzel PM
The international journal of biochemistry & cell biology41(5)1205-15(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Siah2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Siah2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01422	NM_134457.1 Latest version!	Rattus norvegicus siah E3 ubiquitin protein ligase 2 (Siah2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Siah2 ( NM_134457.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_604452.1 Rattus norvegicus siah E3 ubiquitin protein ligase 2 (Siah2), mRNA.

Price & Availability↑

CloneID	ORa01422
Clone ID Related Accession (Same CDS sequence)	NM_134457.1
Accession Version	NM_134457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1555516800000
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase SIAH2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB067815.1. ##Evidence-Data-START## Transcript exon combination :: AB067815.1, AF389477.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCGCC CGTCCTCCAC CGGCCCCAGC GCTAACAAAC CCTGCAGCAA GCAGCCGCCG 
CCGCCTCAGA CCCCGCACGC CCCGTCCCCG GCTGCGCCCC CGGCTGCCGC CACCATCTCG 
GCTGCGGGCC CCGGCTCGTC CGCGGTGCCC GCCGCGGCAG CGGTGATCTC AGGCCCCGGC 
GCCGGCGGCG GGGCCGGCCC AGTGTCCCCG CAGCACCACG AGCTGACCTC GCTGTTCGAG 
TGCCCGGTCT GTTTTGACTA TGTCCTGCCC CCCATTCTGC AGTGCCAGGC CGGGCACCTG 
GTGTGTAACC AATGCCGCCA GAAGTTGAGC TGCTGCCCGA CGTGCAGGGG CGCCCTAACG 
CCCAGCATCA GGAACCTGGC TATGGAGAAG GTCGCCTCGG CAGTTCTGTT TCCCTGTAAG 
TATGCCACCA CGGGCTGTTC CCTGACTCTA CACCATACAG AGAAACCAGA GCATGAAGAC 
ATCTGTGAAT ACCGTCCTTA TTCCTGTCCC TGTCCTGGTG CCTCCTGCAA GTGGCAAGGA 
TCCCTAGAAG CTGTGATGTC CCATCTCATG CATGCCCACA AGAGCATCAC TACCCTTCAG 
GGAGAGGACA TAGTCTTTCT AGCTACAGAC ATTAACCTGC CAGGGGCTGT GGACTGGGTG 
ATGATGCAGT CCTGTTTTGG CCACCACTTC ATGCTGGTAC TCGAGAAGCA AGAGAAGTAC 
GAAGGTCACC AGCAGTTCTT TGCCATCGTC CTCCTCATTG GCACCCGCAA GCAAGCAGAG 
AACTTTGCCT ACAGACTGGA GTTGAATGGG AACCGGAGGA GACTGACCTG GGAGGCCACA 
CCCCGGTCCA TTCATGATGG TGTGGCTGCA GCCATCATGA ACAGTGACTG CCTTGTTTTT 
GACACAGCCA TAGCACATCT CTTTGCAGAT AATGGGAACC TTGGAATCAA TGTCACAATA 
TCTACATGCT GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_604452.1
CDS	239..1216
Translation	MSRPSSTGPSANKPCSKQPPPPQTPHAPSPAAPPAAATISAAGPGSSAVPAAAAVISGPGAGGGAGPVSPQHHELTSLFECPVCFDYVLPPILQCQAGHLVCNQCRQKLSCCPTCRGALTPSIRNLAMEKVASAVLFPCKYATTGCSLTLHHTEKPEHEDICEYRPYSCPCPGASCKWQGSLEAVMSHLMHAHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGHHFMLVLEKQEKYEGHQQFFAIVLLIGTRKQAENFAYRLELNGNRRRLTWEATPRSIHDGVAAAIMNSDCLVFDTAIAHLFADNGNLGINVTISTCCQ

Target ORF information:

RefSeq Version	NM_134457.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus siah E3 ubiquitin protein ligase 2 (Siah2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134457.1

ORF Insert Sequence:

ATGAGCCGCC CGTCCTCCAC CGGCCCCAGC GCTAACAAAC CCTGCAGCAA GCAGCCGCCG 
CCGCCTCAGA CCCCGCACGC CCCGTCCCCG GCTGCGCCCC CGGCTGCCGC CACCATCTCG 
GCTGCGGGCC CCGGCTCGTC CGCGGTGCCC GCCGCGGCAG CGGTGATCTC AGGCCCCGGC 
GCCGGCGGCG GGGCCGGCCC AGTGTCCCCG CAGCACCACG AGCTGACCTC GCTGTTCGAG 
TGCCCGGTCT GTTTTGACTA TGTCCTGCCC CCCATTCTGC AGTGCCAGGC CGGGCACCTG 
GTGTGTAACC AATGCCGCCA GAAGTTGAGC TGCTGCCCGA CGTGCAGGGG CGCCCTAACG 
CCCAGCATCA GGAACCTGGC TATGGAGAAG GTCGCCTCGG CAGTTCTGTT TCCCTGTAAG 
TATGCCACCA CGGGCTGTTC CCTGACTCTA CACCATACAG AGAAACCAGA GCATGAAGAC 
ATCTGTGAAT ACCGTCCTTA TTCCTGTCCC TGTCCTGGTG CCTCCTGCAA GTGGCAAGGA 
TCCCTAGAAG CTGTGATGTC CCATCTCATG CATGCCCACA AGAGCATCAC TACCCTTCAG 
GGAGAGGACA TAGTCTTTCT AGCTACAGAC ATTAACCTGC CAGGGGCTGT GGACTGGGTG 
ATGATGCAGT CCTGTTTTGG CCACCACTTC ATGCTGGTAC TCGAGAAGCA AGAGAAGTAC 
GAAGGTCACC AGCAGTTCTT TGCCATCGTC CTCCTCATTG GCACCCGCAA GCAAGCAGAG 
AACTTTGCCT ACAGACTGGA GTTGAATGGG AACCGGAGGA GACTGACCTG GGAGGCCACA 
CCCCGGTCCA TTCATGATGG TGTGGCTGCA GCCATCATGA ACAGTGACTG CCTTGTTTTT 
GACACAGCCA TAGCACATCT CTTTGCAGAT AATGGGAACC TTGGAATCAA TGTCACAATA 
TCTACATGCT GTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ubiquitin ligase Siah2 regulates RevErbα degradation and the mammalian circadian clock.
Proceedings of the National Academy of Sciences of the United States of America112(40)12420-5(2015 Oct)
DeBruyne JP,Baggs JE,Sato TK,Hogenesch JB

Exchange Factor TBL1 and Arginine Methyltransferase PRMT6 Cooperate in Protecting G Protein Pathway Suppressor 2 (GPS2) from Proteasomal Degradation.
The Journal of biological chemistry290(31)19044-54(2015 Jul)
Huang J,Cardamone MD,Johnson HE,Neault M,Chan M,Floyd ZE,Mallette FA,Perissi V

The Nrf2 regulatory network provides an interface between redox and intermediary metabolism.
Trends in biochemical sciences39(4)199-218(2014 Apr)
Hayes JD,Dinkova-Kostova AT

Possible role of death receptor-mediated apoptosis by the E3 ubiquitin ligases Siah2 and POSH.
Molecular cancer1057(2011 May)
Christian PA,Fiandalo MV,Schwarze SR

Maturation of human dendritic cells is accompanied by functional remodelling of the ubiquitin-proteasome system.
The international journal of biochemistry & cell biology41(5)1205-15(2009 May)
Ebstein F,Lange N,Urban S,Seifert U,Krüger E,Kloetzel PM