Dpyd cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpyd
Entrez Gene ID	99586
Full Name	dihydropyrimidine dehydrogenase
Synonyms	AI315208,DPD,E330028L06Rik
General protein information	Preferred Names dihydropyrimidine dehydrogenase Names dihydropyrimidine dehydrogenase [NADP(+)] DHPDHase dihydrothymine dehydrogenase dihydrouracil dehydrogenase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 G1

mRNA and Protein(s)

mRNA	Protein	Name
XM_011240318.2	XP_011238620.1	dihydropyrimidine dehydrogenase [NADP(+)] isoform X1
NM_170778.2	NP_740748.1	dihydropyrimidine dehydrogenase [NADP(+)]
NM_170778.3	NP_740748.1	dihydropyrimidine dehydrogenase [NADP(+)]

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DPYD	XP_513583.3
Gallus gallus (chicken)	DPYD	XP_426639.3
Xenopus tropicalis (tropical clawed frog)	dpyd	XP_002933845.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001021	XP_003437170.1
Homo sapiens (human)	DPYD	NP_000101.2
Canis lupus familiaris (dog)	DPYD	XP_005621932.1
Mus musculus (house mouse)	Dpyd	NP_740748.1
Danio rerio (zebrafish)	dpydb	NP_998058.1
Drosophila melanogaster (fruit fly)	su(r)	NP_572538.1
Caenorhabditis elegans (roundworm)	dpyd-1	NP_508927.2
Macaca mulatta (Rhesus monkey)	DPYD	XP_001106007.1
Bos taurus (cattle)	DPYD	NP_776466.1
Rattus norvegicus (Norway rat)	Dpyd	NP_112289.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bućan M, Murray SA
Nature537(7621)508-514(2016 09)

Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis.
Maddala R, Nagendran T, Lang RA, Morozov A, Rao PV
Developmental biology406(1)74-91(2015 Oct)

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Tamplin OJ, Cox BJ, Rossant J
Developmental biology360(2)415-25(2011 Dec)

Dihydropyrimidine dehydrogenase circadian rhythm in mouse liver: comparison between enzyme activity and gene expression.
Porsin B, Formento JL, Filipski E, Etienne MC, Francoual M, Renée N, Magné N, Lévi F, Milano G
European journal of cancer (Oxford, England : 1990)39(6)822-8(2003 Apr)

Regulation of dihydropyrimidine dehydrogenase gene expression in regenerating mouse liver.
Yamada H, Fukushima M, Koizumi K, Katakura H, Nakanishi H, Teshima S, Kamiyama Y, Ikenaka K
International journal of oncology22(2)359-64(2003 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpyd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpyd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu71976	XM_011240318.2 Latest version!	Mus musculus dihydropyrimidine dehydrogenase (Dpyd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu02870	NM_170778.3 Latest version!	Mus musculus dihydropyrimidine dehydrogenase (Dpyd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$601.30 $859.00
View Old Accession Versions >>
OMu02870	NM_170778.2	Mus musculus dihydropyrimidine dehydrogenase (Dpyd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$429.50-$601.30 $859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpyd ( NM_170778.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_740748.1 Mus musculus dihydropyrimidine dehydrogenase (Dpyd), mRNA.

Price & Availability↑

CloneID	OMu02870
Clone ID Related Accession (Same CDS sequence)	NM_170778.2 , NM_170778.3
Accession Version	NM_170778.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509379200000
Organism	Mus musculus(house mouse)
Product	dihydropyrimidine dehydrogenase [NADP(+)]
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC122183.4, AC152401.6, AC121544.9, AC161223.4, AC115935.9, AC116409.9, AC115017.11, AC115012.9 and AC110513.6. On Nov 17, 2006 this sequence version replaced NM_170778.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC039699.1, SRR1660821.132334.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGTG TGCTGAGCAG GGACGCGCCG GACATCGAGA GTATCCTGGC TTTAAACCCT 
CGAGTACAAG CTCATGCAAC TCTGCGTTCC ACCGCAGCCA AGAAACTAGA CAAGAAACAC 
TGGAAAAGGA ACACGGACAA GAACTGCTTT ACCTGTGAGA AGCTGGAGAG TAATTTTGAT 
GACATCAAGC ACACGACTCT TGGTGAGCGA GGAGCTCTCC GAGAAGCAGT GAGATGCCTG 
AAGTGCGCAG ATGCTCCCTG CCAGAAGAGC TGTCCCACAT CTCTTGACAT TAAATCATTT 
ATCACAAGTA TCGCCAACAA GAACTACTAT GGTGCAGCTA AGCTGATTTT TTCTGATAAC 
CCTCTTGGTC TTACTTGTGG AATGGTTTGT CCAACATCTG ACCTCTGTGT TGGAGGATGC 
AACTTACATG CTGCTGAAGA GGGACCAATT AATATTGGTG GGCTGCAGCA GTTTGCTACT 
GAGGTGTTCA AAGCAATGAA CATCCCACAG ATCAGAAACC CATCGCTGCC TCCTCCGGAA 
CATATGCCGG AAGCCTACTC AGCAAAAATT GCACTTTTTG GTGCAGGGCC TGCGAGTATA 
AGCTGTGCCT CCTTTTTGGC TCGGTTGGGC TATTCCAACA TTACTATATT CGAAAAACAA 
GAATATGTTG GTGGTTTAAG CACTTCTGAA ATCCCTCAGT TTCGACTCCC ATATGACGTC 
GTGAATTTTG AGATTGAGCT CATGAAGGAC CTTGGTGTAA AGATAATTTG TGGTAAAAGC 
CTTTCCACCG ATGAAATGAC TCTTAGCTCT TTGAAAGAAA ATGGCTACAG AGCTGCATTT 
ATTGGAATAG GCTTGCCAGA ACCCAAAAAG GACCATATTT TCCAAGGCTT GACACAAGTC 
CAGGGATTTT ACACATCTAA AGACTTTCTG CCACTTGTCG CCAAAAGCAG TAAAACAGGA 
ATGTGCGCCT GTCACTCTCC ATTGCCATCC ATAAGGGGAG CCGTGATTGT ACTCGGAGCT 
GGAGATACTG CGTTTGACTG TGCAACATCC GCTCTGCGCT GTGGAGCCCT TCGCGTGTTC 
ATCGTCTTCA GAAAGGGCTT TGTTAATATT CGAGCTGTTC CAGAGGAGAT GGAGCTCGCT 
AAGGAAGAGA AATGTGAATT TTTGCCTTTC CTCTCGCCAC GGAAGGTTAT AGTCAAAGAT 
GGAAAGATTG TGGCAATGCA ATTTGTTCGA ACTGAGCAAG ATGAAACCGG AAACTGGGTG 
GAAGATGAAG AGCAGACGGT GCGGCTAAAG GCTGATGTGG TTATTAGTGC CTTTGGATCT 
GTCCTGGAGG ATCCCAAAGT GAAAGAAGCA TTGAGTCCCA TCAAGTTTAA CAGATGGGGT 
CTCCCAGAAG TAAATCCAGA AACCATGCAA ACCAGTGAAC CATGGGTGTT TGCAGGTGGT 
GATGTTGTGG GTATGGCTAA CACCACAGTG GAATCTGTCA ACGATGGAAA GCAAGCTTCA 
TGGTACATTC ACAAGCACAT ACAGGCACAA TATGGAACCT CAGTGCCTTC TCAGCCTACA 
ATGCCCCTAT TTTACACTCC GGTTGACTTA GTGGACATCA GCGTGGAAAT GGCAGGGTTG 
AGGTTTCCCA ATCCCTTTGG CCTTGCCAGT GCGACACCAG CCACTAGCAC ACCAATGATT 
CGAAGGGCCT TTGAAGCTGG CTGGGGTTTT GCTCTGACCA AAACTTTCTC TCTTGATAAG 
GACATCGTGA CAAACGTCTC ACCAAGAATC ATCCGGGGGA CAACTTCTGG CCCCTTGTAT 
GGCCCTGGAC AAAGCTCCTT TCTTAACATT GAGCTCATCA GTGAGAAAAC AGCTGCATAT 
TGGTGTCACA GTGTCACCGA ACTAAAGGCT GACTTCCCGG ACAATATCCT GATCGCCAGC 
ATCATGTGCA GTTACAACAA GAGTGACTGG ATGGAACTCT CCAAAATGGC TGAGGCTTCT 
GGAGCAGATG CCCTGGAGTT AAATTTATCG TGTCCACATG GCATGGGGGA GAGAGGAATG 
GGCCTGGCTT GTGGGCAGGA TCCAGAGCTG GTGAGGAACA TCTGTCGCTG GGTTAGGCAA 
GCTGTTCGGG TTCCATTTTT TGCCAAGTTG ACCCCAAATG TCACTGATAT TGTAAGCATC 
GCAAGAGCAG CAAAGGAAGG TGGAGCAGAT GGTGTAACAG CCACCAACAC CGTCTCGGGT 
CTGATGGGAC TGAAAGCTGA TGGCACACCC TGGCCTGCTG TGGGCATTGG AAGGAGGACT 
ACATATGGAG GAGTGTCAGG AACTGCTATC AGGCCCATTG CTTTGAGAGC TGTGACCGCC 
ATTGCCCGTG CTTTGCCTGG GTTTCCTATT TTGGCCACAG GTGGAATTGA CTCAGCTGAA 
AGTGGACTTC AGTTTCTTCA TAGTGGTGCT TCAGTTCTTC AGGTATGCAG CGCTATTCAG 
AATCAGGACT TCACTGTGAT TGAAGATTAC TGCACTGGAC TCAAAGCCCT GCTTTATCTG 
AAGAGCATTG AAGAGTTAGC AGACTGGGAT GGTCAGAGTC CACCCATTAT AAGTCATCAG 
AAAGGGAAAC CAGTTCCACG CGTTGCTGAG CTCATGGGAC AGAAACTGCC CAGCTTTGGA 
CCTTACCTTG AACAGCGCAA GAAAATCATA GCAGCAAGCA AAATCAGACA GAAAGATCAA 
AACACAGCTT GCTCACCTCT CCAGAGAAAG CACTTTAACT CGCAAAAGCC TATTCCTGCC 
ATCAAGGATG TAATTGGAAA ATCACTGCAA TACCTGGGAA CATTTGGTGA GATGAGCATC 
ATGGAACAAG TTGTGGCCCT GATCGATGAG GAAATGTGCA TCAATTGCGG CAAATGTTAC 
ATGACCTGTA ATGACTCTGG CTACCAGGCT ATCCAGTTCG ATCCAGAAAC TCACCTGCCT 
ACCGTTAGCG ACACATGTAC AGGCTGTACT CTCTGCCTCA GCGTCTGCCC TATTATGGAC 
TGTATCAGGA TGGTTTCCAG GGCAACACCT TATCAACCAA AGAGAGGCCT ACCATTAGCC 
GTGAAGCCGG TGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_740748.1
CDS	99..3176
Translation	MAGVLSRDAPDIESILALNPRVQAHATLRSTAAKKLDKKHWKRNTDKNCFTCEKLESNFDDIKHTTLGERGALREAVRCLKCADAPCQKSCPTSLDIKSFITSIANKNYYGAAKLIFSDNPLGLTCGMVCPTSDLCVGGCNLHAAEEGPINIGGLQQFATEVFKAMNIPQIRNPSLPPPEHMPEAYSAKIALFGAGPASISCASFLARLGYSNITIFEKQEYVGGLSTSEIPQFRLPYDVVNFEIELMKDLGVKIICGKSLSTDEMTLSSLKENGYRAAFIGIGLPEPKKDHIFQGLTQVQGFYTSKDFLPLVAKSSKTGMCACHSPLPSIRGAVIVLGAGDTAFDCATSALRCGALRVFIVFRKGFVNIRAVPEEMELAKEEKCEFLPFLSPRKVIVKDGKIVAMQFVRTEQDETGNWVEDEEQTVRLKADVVISAFGSVLEDPKVKEALSPIKFNRWGLPEVNPETMQTSEPWVFAGGDVVGMANTTVESVNDGKQASWYIHKHIQAQYGTSVPSQPTMPLFYTPVDLVDISVEMAGLRFPNPFGLASATPATSTPMIRRAFEAGWGFALTKTFSLDKDIVTNVSPRIIRGTTSGPLYGPGQSSFLNIELISEKTAAYWCHSVTELKADFPDNILIASIMCSYNKSDWMELSKMAEASGADALELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQAVRVPFFAKLTPNVTDIVSIARAAKEGGADGVTATNTVSGLMGLKADGTPWPAVGIGRRTTYGGVSGTAIRPIALRAVTAIARALPGFPILATGGIDSAESGLQFLHSGASVLQVCSAIQNQDFTVIEDYCTGLKALLYLKSIEELADWDGQSPPIISHQKGKPVPRVAELMGQKLPSFGPYLEQRKKIIAASKIRQKDQNTACSPLQRKHFNSQKPIPAIKDVIGKSLQYLGTFGEMSIMEQVVALIDEEMCINCGKCYMTCNDSGYQAIQFDPETHLPTVSDTCTGCTLCLSVCPIMDCIRMVSRATPYQPKRGLPLAVKPVC

Target ORF information:

RefSeq Version	NM_170778.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dihydropyrimidine dehydrogenase (Dpyd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170778.2

ORF Insert Sequence:

ATGGCGGGTG TGCTGAGCAG GGACGCGCCG GACATCGAGA GTATCCTGGC TTTAAACCCT 
CGAGTACAAG CTCATGCAAC TCTGCGTTCC ACCGCAGCCA AGAAACTAGA CAAGAAACAC 
TGGAAAAGGA ACACGGACAA GAACTGCTTT ACCTGTGAGA AGCTGGAGAG TAATTTTGAT 
GACATCAAGC ACACGACTCT TGGTGAGCGA GGAGCTCTCC GAGAAGCAGT GAGATGCCTG 
AAGTGCGCAG ATGCTCCCTG CCAGAAGAGC TGTCCCACAT CTCTTGACAT TAAATCATTT 
ATCACAAGTA TCGCCAACAA GAACTACTAT GGTGCAGCTA AGCTGATTTT TTCTGATAAC 
CCTCTTGGTC TTACTTGTGG AATGGTTTGT CCAACATCTG ACCTCTGTGT TGGAGGATGC 
AACTTACATG CTGCTGAAGA GGGACCAATT AATATTGGTG GGCTGCAGCA GTTTGCTACT 
GAGGTGTTCA AAGCAATGAA CATCCCACAG ATCAGAAACC CATCGCTGCC TCCTCCGGAA 
CATATGCCGG AAGCCTACTC AGCAAAAATT GCACTTTTTG GTGCAGGGCC TGCGAGTATA 
AGCTGTGCCT CCTTTTTGGC TCGGTTGGGC TATTCCAACA TTACTATATT CGAAAAACAA 
GAATATGTTG GTGGTTTAAG CACTTCTGAA ATCCCTCAGT TTCGACTCCC ATATGACGTC 
GTGAATTTTG AGATTGAGCT CATGAAGGAC CTTGGTGTAA AGATAATTTG TGGTAAAAGC 
CTTTCCACCG ATGAAATGAC TCTTAGCTCT TTGAAAGAAA ATGGCTACAG AGCTGCATTT 
ATTGGAATAG GCTTGCCAGA ACCCAAAAAG GACCATATTT TCCAAGGCTT GACACAAGTC 
CAGGGATTTT ACACATCTAA AGACTTTCTG CCACTTGTCG CCAAAAGCAG TAAAACAGGA 
ATGTGCGCCT GTCACTCTCC ATTGCCATCC ATAAGGGGAG CCGTGATTGT ACTCGGAGCT 
GGAGATACTG CGTTTGACTG TGCAACATCC GCTCTGCGCT GTGGAGCCCT TCGCGTGTTC 
ATCGTCTTCA GAAAGGGCTT TGTTAATATT CGAGCTGTTC CAGAGGAGAT GGAGCTCGCT 
AAGGAAGAGA AATGTGAATT TTTGCCTTTC CTCTCGCCAC GGAAGGTTAT AGTCAAAGAT 
GGAAAGATTG TGGCAATGCA ATTTGTTCGA ACTGAGCAAG ATGAAACCGG AAACTGGGTG 
GAAGATGAAG AGCAGACGGT GCGGCTAAAG GCTGATGTGG TTATTAGTGC CTTTGGATCT 
GTCCTGGAGG ATCCCAAAGT GAAAGAAGCA TTGAGTCCCA TCAAGTTTAA CAGATGGGGT 
CTCCCAGAAG TAAATCCAGA AACCATGCAA ACCAGTGAAC CATGGGTGTT TGCAGGTGGT 
GATGTTGTGG GTATGGCTAA CACCACAGTG GAATCTGTCA ACGATGGAAA GCAAGCTTCA 
TGGTACATTC ACAAGCACAT ACAGGCACAA TATGGAACCT CAGTGCCTTC TCAGCCTACA 
ATGCCCCTAT TTTACACTCC GGTTGACTTA GTGGACATCA GCGTGGAAAT GGCAGGGTTG 
AGGTTTCCCA ATCCCTTTGG CCTTGCCAGT GCGACACCAG CCACTAGCAC ACCAATGATT 
CGAAGGGCCT TTGAAGCTGG CTGGGGTTTT GCTCTGACCA AAACTTTCTC TCTTGATAAG 
GACATCGTGA CAAACGTCTC ACCAAGAATC ATCCGGGGGA CAACTTCTGG CCCCTTGTAT 
GGCCCTGGAC AAAGCTCCTT TCTTAACATT GAGCTCATCA GTGAGAAAAC AGCTGCATAT 
TGGTGTCACA GTGTCACCGA ACTAAAGGCT GACTTCCCGG ACAATATCCT GATCGCCAGC 
ATCATGTGCA GTTACAACAA GAGTGACTGG ATGGAACTCT CCAAAATGGC TGAGGCTTCT 
GGAGCAGATG CCCTGGAGTT AAATTTATCG TGTCCACATG GCATGGGGGA GAGAGGAATG 
GGCCTGGCTT GTGGGCAGGA TCCAGAGCTG GTGAGGAACA TCTGTCGCTG GGTTAGGCAA 
GCTGTTCGGG TTCCATTTTT TGCCAAGTTG ACCCCAAATG TCACTGATAT TGTAAGCATC 
GCAAGAGCAG CAAAGGAAGG TGGAGCAGAT GGTGTAACAG CCACCAACAC CGTCTCGGGT 
CTGATGGGAC TGAAAGCTGA TGGCACACCC TGGCCTGCTG TGGGCATTGG AAGGAGGACT 
ACATATGGAG GAGTGTCAGG AACTGCTATC AGGCCCATTG CTTTGAGAGC TGTGACCGCC 
ATTGCCCGTG CTTTGCCTGG GTTTCCTATT TTGGCCACAG GTGGAATTGA CTCAGCTGAA 
AGTGGACTTC AGTTTCTTCA TAGTGGTGCT TCAGTTCTTC AGGTATGCAG CGCTATTCAG 
AATCAGGACT TCACTGTGAT TGAAGATTAC TGCACTGGAC TCAAAGCCCT GCTTTATCTG 
AAGAGCATTG AAGAGTTAGC AGACTGGGAT GGTCAGAGTC CACCCATTAT AAGTCATCAG 
AAAGGGAAAC CAGTTCCACG CGTTGCTGAG CTCATGGGAC AGAAACTGCC CAGCTTTGGA 
CCTTACCTTG AACAGCGCAA GAAAATCATA GCAGCAAGCA AAATCAGACA GAAAGATCAA 
AACACAGCTT GCTCACCTCT CCAGAGAAAG CACTTTAACT CGCAAAAGCC TATTCCTGCC 
ATCAAGGATG TAATTGGAAA ATCACTGCAA TACCTGGGAA CATTTGGTGA GATGAGCATC 
ATGGAACAAG TTGTGGCCCT GATCGATGAG GAAATGTGCA TCAATTGCGG CAAATGTTAC 
ATGACCTGTA ATGACTCTGG CTACCAGGCT ATCCAGTTCG ATCCAGAAAC TCACCTGCCT 
ACCGTTAGCG ACACATGTAC AGGCTGTACT CTCTGCCTCA GCGTCTGCCC TATTATGGAC 
TGTATCAGGA TGGTTTCCAG GGCAACACCT TATCAACCAA AGAGAGGCCT ACCATTAGCC 
GTGAAGCCGG TGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpyd ( XM_011240318.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238620.1 Mus musculus dihydropyrimidine dehydrogenase (Dpyd), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu71976
Clone ID Related Accession (Same CDS sequence)	XM_011240318.2
Accession Version	XM_011240318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	dihydropyrimidine dehydrogenase [NADP(+)] isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGTG TGCTGAGCAG GGACGCGCCG GACATCGAGA GTATCCTGGC TTTAAACCCT 
CGAGTACAAG CTCATGCAAC TCTGCGTTCC ACCGCAGCCA AGAAACTAGA CAAGAAACAC 
TGGAAAAGGA ACACGGACAA GAACTGCTTT ACCTGTGAGA AGCTGGAGAG TAATTTTGAT 
GACATCAAGC ACACGACTCT TGGTGAGCGA GGAGCTCTCC GAGAAGCAGT GAGATGCCTG 
AAGTGCGCAG ATGCTCCCTG CCAGAAGAGC TGTCCCACAT CTCTTGACAT TAAATCATTT 
ATCACAAGTA TCGCCAACAA GAACTACTAT GGTGCAGCTA AGCTGATTTT TTCTGATAAC 
CCTCTTGGTC TTACTTGTGG AATGGTTTGT CCAACATCTG ACCTCTGTGT TGGAGGATGC 
AACTTACATG CTGCTGAAGA GGGACCAATT AATATTGGTG GGCTGCAGCA GTTTGCTACT 
GAGGTGTTCA AAGCAATGAA CATCCCACAG ATCAGAAACC CATCGCTGCC TCCTCCGGAA 
CATATGCCGG AAGCCTACTC AGCAAAAATT GCACTTTTTG GTGCAGGGCC TGCGAGTATA 
AGCTGTGCCT CCTTTTTGGC TCGGTTGGGC TATTCCAACA TTACTATATT CGAAAAACAA 
GAATATGTTG GTGGTTTAAG CACTTCTGAA ATCCCTCAGT TTCGACTCCC ATATGACGTC 
GTGAATTTTG AGATTGAGCT CATGAAGGAC CTTGGTGTAA AGATAATTTG TGGTAAAAGC 
CTTTCCACCG ATGAAATGAC TCTTAGCTCT TTGAAAGAAA ATGGCTACAG AGCTGCATTT 
ATTGGAATAG GCTTGCCAGA ACCCAAAAAG GACCATATTT TCCAAGGCTT GACACAAGTC 
CAGGGATTTT ACACATCTAA AGACTTTCTG CCACTTGTCG CCAAAAGCAG TAAAACAGGA 
ATGTGCGCCT GTCACTCTCC ATTGCCATCC ATAAGGGGAG CCGTGATTGT ACTCGGAGCT 
GGAGATACTG CGTTTGACTG TGCAACATCC GCTCTGCGCT GTGGAGCCCT TCGCGTGTTC 
ATCGTCTTCA GAAAGGGCTT TGTTAATATT CGAGCTGTTC CAGAGGAGAT GGAGCTCGCT 
AAGGAAGAGA AATGTGAATT TTTGCCTTTC CTCTCGCCAC GGAAGGTTAT AGTCAAAGAT 
GGAAAGATTG TGGCAATGCA ATTTGTTCGA ACTGAGCAAG ATGAAACCGG AAACTGGGTG 
GAAGATGAAG AGCAGACGGT GCGGCTAAAG GCTGATGTGG TTATTAGTGC CTTTGGATCT 
GTCCTGGAGG ATCCCAAAGT GAAAGAAGCA TTGAGTCCCA TCAAGTTTAA CAGATGGGGT 
CTCCCAGAAG TAAATCCAGA AACCATGCAA ACCAGTGAAC CATGGGTGTT TGCAGGTGGT 
GATGTTGTGG GTATGGCTAA CACCACAGTG GAATCTGTCA ACGATGGAAA GCAAGCTTCA 
TGGTACATTC ACAAGCACAT ACAGGCACAA TATGGAACCT CAGTGCCTTC TCAGCCTACA 
ATGCCCCTAT TTTACACTCC GGTTGACTTA GTGGACATCA GCGTGGAAAT GGCAGGGTTG 
AGGTTTCCCA ATCCCTTTGG CCTTGCCAGT GCGACACCAG CCACTAGCAC ACCAATGATT 
CGAAGGGCCT TTGAAGCTGG CTGGGGTTTT GCTCTGACCA AAACTTTCTC TCTTGATAAG 
GCCTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238620.1
CDS	168..1916
Translation	MAGVLSRDAPDIESILALNPRVQAHATLRSTAAKKLDKKHWKRNTDKNCFTCEKLESNFDDIKHTTLGERGALREAVRCLKCADAPCQKSCPTSLDIKSFITSIANKNYYGAAKLIFSDNPLGLTCGMVCPTSDLCVGGCNLHAAEEGPINIGGLQQFATEVFKAMNIPQIRNPSLPPPEHMPEAYSAKIALFGAGPASISCASFLARLGYSNITIFEKQEYVGGLSTSEIPQFRLPYDVVNFEIELMKDLGVKIICGKSLSTDEMTLSSLKENGYRAAFIGIGLPEPKKDHIFQGLTQVQGFYTSKDFLPLVAKSSKTGMCACHSPLPSIRGAVIVLGAGDTAFDCATSALRCGALRVFIVFRKGFVNIRAVPEEMELAKEEKCEFLPFLSPRKVIVKDGKIVAMQFVRTEQDETGNWVEDEEQTVRLKADVVISAFGSVLEDPKVKEALSPIKFNRWGLPEVNPETMQTSEPWVFAGGDVVGMANTTVESVNDGKQASWYIHKHIQAQYGTSVPSQPTMPLFYTPVDLVDISVEMAGLRFPNPFGLASATPATSTPMIRRAFEAGWGFALTKTFSLDKAL

Target ORF information:

RefSeq Version	XM_011240318.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dihydropyrimidine dehydrogenase (Dpyd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240318.2

ORF Insert Sequence:

ATGGCGGGTG TGCTGAGCAG GGACGCGCCG GACATCGAGA GTATCCTGGC TTTAAACCCT 
CGAGTACAAG CTCATGCAAC TCTGCGTTCC ACCGCAGCCA AGAAACTAGA CAAGAAACAC 
TGGAAAAGGA ACACGGACAA GAACTGCTTT ACCTGTGAGA AGCTGGAGAG TAATTTTGAT 
GACATCAAGC ACACGACTCT TGGTGAGCGA GGAGCTCTCC GAGAAGCAGT GAGATGCCTG 
AAGTGCGCAG ATGCTCCCTG CCAGAAGAGC TGTCCCACAT CTCTTGACAT TAAATCATTT 
ATCACAAGTA TCGCCAACAA GAACTACTAT GGTGCAGCTA AGCTGATTTT TTCTGATAAC 
CCTCTTGGTC TTACTTGTGG AATGGTTTGT CCAACATCTG ACCTCTGTGT TGGAGGATGC 
AACTTACATG CTGCTGAAGA GGGACCAATT AATATTGGTG GGCTGCAGCA GTTTGCTACT 
GAGGTGTTCA AAGCAATGAA CATCCCACAG ATCAGAAACC CATCGCTGCC TCCTCCGGAA 
CATATGCCGG AAGCCTACTC AGCAAAAATT GCACTTTTTG GTGCAGGGCC TGCGAGTATA 
AGCTGTGCCT CCTTTTTGGC TCGGTTGGGC TATTCCAACA TTACTATATT CGAAAAACAA 
GAATATGTTG GTGGTTTAAG CACTTCTGAA ATCCCTCAGT TTCGACTCCC ATATGACGTC 
GTGAATTTTG AGATTGAGCT CATGAAGGAC CTTGGTGTAA AGATAATTTG TGGTAAAAGC 
CTTTCCACCG ATGAAATGAC TCTTAGCTCT TTGAAAGAAA ATGGCTACAG AGCTGCATTT 
ATTGGAATAG GCTTGCCAGA ACCCAAAAAG GACCATATTT TCCAAGGCTT GACACAAGTC 
CAGGGATTTT ACACATCTAA AGACTTTCTG CCACTTGTCG CCAAAAGCAG TAAAACAGGA 
ATGTGCGCCT GTCACTCTCC ATTGCCATCC ATAAGGGGAG CCGTGATTGT ACTCGGAGCT 
GGAGATACTG CGTTTGACTG TGCAACATCC GCTCTGCGCT GTGGAGCCCT TCGCGTGTTC 
ATCGTCTTCA GAAAGGGCTT TGTTAATATT CGAGCTGTTC CAGAGGAGAT GGAGCTCGCT 
AAGGAAGAGA AATGTGAATT TTTGCCTTTC CTCTCGCCAC GGAAGGTTAT AGTCAAAGAT 
GGAAAGATTG TGGCAATGCA ATTTGTTCGA ACTGAGCAAG ATGAAACCGG AAACTGGGTG 
GAAGATGAAG AGCAGACGGT GCGGCTAAAG GCTGATGTGG TTATTAGTGC CTTTGGATCT 
GTCCTGGAGG ATCCCAAAGT GAAAGAAGCA TTGAGTCCCA TCAAGTTTAA CAGATGGGGT 
CTCCCAGAAG TAAATCCAGA AACCATGCAA ACCAGTGAAC CATGGGTGTT TGCAGGTGGT 
GATGTTGTGG GTATGGCTAA CACCACAGTG GAATCTGTCA ACGATGGAAA GCAAGCTTCA 
TGGTACATTC ACAAGCACAT ACAGGCACAA TATGGAACCT CAGTGCCTTC TCAGCCTACA 
ATGCCCCTAT TTTACACTCC GGTTGACTTA GTGGACATCA GCGTGGAAAT GGCAGGGTTG 
AGGTTTCCCA ATCCCTTTGG CCTTGCCAGT GCGACACCAG CCACTAGCAC ACCAATGATT 
CGAAGGGCCT TTGAAGCTGG CTGGGGTTTT GCTCTGACCA AAACTTTCTC TCTTGATAAG 
GCCTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpyd ( NM_170778.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_740748.1 Mus musculus dihydropyrimidine dehydrogenase (Dpyd), mRNA.

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CloneID	OMu02870
Clone ID Related Accession (Same CDS sequence)	NM_170778.2 , NM_170778.3
Accession Version	NM_170778.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571760000000
Organism	Mus musculus(house mouse)
Product	dihydropyrimidine dehydrogenase [NADP(+)]
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122183.4, AC152401.6, AC121544.9, AC161223.4, AC115935.9, AC116409.9, AC115017.11, AC115012.9 and AC110513.6. On Apr 12, 2018 this sequence version replaced NM_170778.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC039699.1, SRR1660821.132334.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGTG TGCTGAGCAG GGACGCGCCG GACATCGAGA GTATCCTGGC TTTAAACCCT 
CGAGTACAAG CTCATGCAAC TCTGCGTTCC ACCGCAGCCA AGAAACTAGA CAAGAAACAC 
TGGAAAAGGA ACACGGACAA GAACTGCTTT ACCTGTGAGA AGCTGGAGAG TAATTTTGAT 
GACATCAAGC ACACGACTCT TGGTGAGCGA GGAGCTCTCC GAGAAGCAGT GAGATGCCTG 
AAGTGCGCAG ATGCTCCCTG CCAGAAGAGC TGTCCCACAT CTCTTGACAT TAAATCATTT 
ATCACAAGTA TCGCCAACAA GAACTACTAT GGTGCAGCTA AGCTGATTTT TTCTGATAAC 
CCTCTTGGTC TTACTTGTGG AATGGTTTGT CCAACATCTG ACCTCTGTGT TGGAGGATGC 
AACTTACATG CTGCTGAAGA GGGACCAATT AATATTGGTG GGCTGCAGCA GTTTGCTACT 
GAGGTGTTCA AAGCAATGAA CATCCCACAG ATCAGAAACC CATCGCTGCC TCCTCCGGAA 
CATATGCCGG AAGCCTACTC AGCAAAAATT GCACTTTTTG GTGCAGGGCC TGCGAGTATA 
AGCTGTGCCT CCTTTTTGGC TCGGTTGGGC TATTCCAACA TTACTATATT CGAAAAACAA 
GAATATGTTG GTGGTTTAAG CACTTCTGAA ATCCCTCAGT TTCGACTCCC ATATGACGTC 
GTGAATTTTG AGATTGAGCT CATGAAGGAC CTTGGTGTAA AGATAATTTG TGGTAAAAGC 
CTTTCCACCG ATGAAATGAC TCTTAGCTCT TTGAAAGAAA ATGGCTACAG AGCTGCATTT 
ATTGGAATAG GCTTGCCAGA ACCCAAAAAG GACCATATTT TCCAAGGCTT GACACAAGTC 
CAGGGATTTT ACACATCTAA AGACTTTCTG CCACTTGTCG CCAAAAGCAG TAAAACAGGA 
ATGTGCGCCT GTCACTCTCC ATTGCCATCC ATAAGGGGAG CCGTGATTGT ACTCGGAGCT 
GGAGATACTG CGTTTGACTG TGCAACATCC GCTCTGCGCT GTGGAGCCCT TCGCGTGTTC 
ATCGTCTTCA GAAAGGGCTT TGTTAATATT CGAGCTGTTC CAGAGGAGAT GGAGCTCGCT 
AAGGAAGAGA AATGTGAATT TTTGCCTTTC CTCTCGCCAC GGAAGGTTAT AGTCAAAGAT 
GGAAAGATTG TGGCAATGCA ATTTGTTCGA ACTGAGCAAG ATGAAACCGG AAACTGGGTG 
GAAGATGAAG AGCAGACGGT GCGGCTAAAG GCTGATGTGG TTATTAGTGC CTTTGGATCT 
GTCCTGGAGG ATCCCAAAGT GAAAGAAGCA TTGAGTCCCA TCAAGTTTAA CAGATGGGGT 
CTCCCAGAAG TAAATCCAGA AACCATGCAA ACCAGTGAAC CATGGGTGTT TGCAGGTGGT 
GATGTTGTGG GTATGGCTAA CACCACAGTG GAATCTGTCA ACGATGGAAA GCAAGCTTCA 
TGGTACATTC ACAAGCACAT ACAGGCACAA TATGGAACCT CAGTGCCTTC TCAGCCTACA 
ATGCCCCTAT TTTACACTCC GGTTGACTTA GTGGACATCA GCGTGGAAAT GGCAGGGTTG 
AGGTTTCCCA ATCCCTTTGG CCTTGCCAGT GCGACACCAG CCACTAGCAC ACCAATGATT 
CGAAGGGCCT TTGAAGCTGG CTGGGGTTTT GCTCTGACCA AAACTTTCTC TCTTGATAAG 
GACATCGTGA CAAACGTCTC ACCAAGAATC ATCCGGGGGA CAACTTCTGG CCCCTTGTAT 
GGCCCTGGAC AAAGCTCCTT TCTTAACATT GAGCTCATCA GTGAGAAAAC AGCTGCATAT 
TGGTGTCACA GTGTCACCGA ACTAAAGGCT GACTTCCCGG ACAATATCCT GATCGCCAGC 
ATCATGTGCA GTTACAACAA GAGTGACTGG ATGGAACTCT CCAAAATGGC TGAGGCTTCT 
GGAGCAGATG CCCTGGAGTT AAATTTATCG TGTCCACATG GCATGGGGGA GAGAGGAATG 
GGCCTGGCTT GTGGGCAGGA TCCAGAGCTG GTGAGGAACA TCTGTCGCTG GGTTAGGCAA 
GCTGTTCGGG TTCCATTTTT TGCCAAGTTG ACCCCAAATG TCACTGATAT TGTAAGCATC 
GCAAGAGCAG CAAAGGAAGG TGGAGCAGAT GGTGTAACAG CCACCAACAC CGTCTCGGGT 
CTGATGGGAC TGAAAGCTGA TGGCACACCC TGGCCTGCTG TGGGCATTGG AAGGAGGACT 
ACATATGGAG GAGTGTCAGG AACTGCTATC AGGCCCATTG CTTTGAGAGC TGTGACCGCC 
ATTGCCCGTG CTTTGCCTGG GTTTCCTATT TTGGCCACAG GTGGAATTGA CTCAGCTGAA 
AGTGGACTTC AGTTTCTTCA TAGTGGTGCT TCAGTTCTTC AGGTATGCAG CGCTATTCAG 
AATCAGGACT TCACTGTGAT TGAAGATTAC TGCACTGGAC TCAAAGCCCT GCTTTATCTG 
AAGAGCATTG AAGAGTTAGC AGACTGGGAT GGTCAGAGTC CACCCATTAT AAGTCATCAG 
AAAGGGAAAC CAGTTCCACG CGTTGCTGAG CTCATGGGAC AGAAACTGCC CAGCTTTGGA 
CCTTACCTTG AACAGCGCAA GAAAATCATA GCAGCAAGCA AAATCAGACA GAAAGATCAA 
AACACAGCTT GCTCACCTCT CCAGAGAAAG CACTTTAACT CGCAAAAGCC TATTCCTGCC 
ATCAAGGATG TAATTGGAAA ATCACTGCAA TACCTGGGAA CATTTGGTGA GATGAGCATC 
ATGGAACAAG TTGTGGCCCT GATCGATGAG GAAATGTGCA TCAATTGCGG CAAATGTTAC 
ATGACCTGTA ATGACTCTGG CTACCAGGCT ATCCAGTTCG ATCCAGAAAC TCACCTGCCT 
ACCGTTAGCG ACACATGTAC AGGCTGTACT CTCTGCCTCA GCGTCTGCCC TATTATGGAC 
TGTATCAGGA TGGTTTCCAG GGCAACACCT TATCAACCAA AGAGAGGCCT ACCATTAGCC 
GTGAAGCCGG TGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_740748.1
CDS	97..3174
Translation	MAGVLSRDAPDIESILALNPRVQAHATLRSTAAKKLDKKHWKRNTDKNCFTCEKLESNFDDIKHTTLGERGALREAVRCLKCADAPCQKSCPTSLDIKSFITSIANKNYYGAAKLIFSDNPLGLTCGMVCPTSDLCVGGCNLHAAEEGPINIGGLQQFATEVFKAMNIPQIRNPSLPPPEHMPEAYSAKIALFGAGPASISCASFLARLGYSNITIFEKQEYVGGLSTSEIPQFRLPYDVVNFEIELMKDLGVKIICGKSLSTDEMTLSSLKENGYRAAFIGIGLPEPKKDHIFQGLTQVQGFYTSKDFLPLVAKSSKTGMCACHSPLPSIRGAVIVLGAGDTAFDCATSALRCGALRVFIVFRKGFVNIRAVPEEMELAKEEKCEFLPFLSPRKVIVKDGKIVAMQFVRTEQDETGNWVEDEEQTVRLKADVVISAFGSVLEDPKVKEALSPIKFNRWGLPEVNPETMQTSEPWVFAGGDVVGMANTTVESVNDGKQASWYIHKHIQAQYGTSVPSQPTMPLFYTPVDLVDISVEMAGLRFPNPFGLASATPATSTPMIRRAFEAGWGFALTKTFSLDKDIVTNVSPRIIRGTTSGPLYGPGQSSFLNIELISEKTAAYWCHSVTELKADFPDNILIASIMCSYNKSDWMELSKMAEASGADALELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQAVRVPFFAKLTPNVTDIVSIARAAKEGGADGVTATNTVSGLMGLKADGTPWPAVGIGRRTTYGGVSGTAIRPIALRAVTAIARALPGFPILATGGIDSAESGLQFLHSGASVLQVCSAIQNQDFTVIEDYCTGLKALLYLKSIEELADWDGQSPPIISHQKGKPVPRVAELMGQKLPSFGPYLEQRKKIIAASKIRQKDQNTACSPLQRKHFNSQKPIPAIKDVIGKSLQYLGTFGEMSIMEQVVALIDEEMCINCGKCYMTCNDSGYQAIQFDPETHLPTVSDTCTGCTLCLSVCPIMDCIRMVSRATPYQPKRGLPLAVKPVC

Target ORF information:

RefSeq Version	NM_170778.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dihydropyrimidine dehydrogenase (Dpyd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170778.3

ORF Insert Sequence:

ATGGCGGGTG TGCTGAGCAG GGACGCGCCG GACATCGAGA GTATCCTGGC TTTAAACCCT 
CGAGTACAAG CTCATGCAAC TCTGCGTTCC ACCGCAGCCA AGAAACTAGA CAAGAAACAC 
TGGAAAAGGA ACACGGACAA GAACTGCTTT ACCTGTGAGA AGCTGGAGAG TAATTTTGAT 
GACATCAAGC ACACGACTCT TGGTGAGCGA GGAGCTCTCC GAGAAGCAGT GAGATGCCTG 
AAGTGCGCAG ATGCTCCCTG CCAGAAGAGC TGTCCCACAT CTCTTGACAT TAAATCATTT 
ATCACAAGTA TCGCCAACAA GAACTACTAT GGTGCAGCTA AGCTGATTTT TTCTGATAAC 
CCTCTTGGTC TTACTTGTGG AATGGTTTGT CCAACATCTG ACCTCTGTGT TGGAGGATGC 
AACTTACATG CTGCTGAAGA GGGACCAATT AATATTGGTG GGCTGCAGCA GTTTGCTACT 
GAGGTGTTCA AAGCAATGAA CATCCCACAG ATCAGAAACC CATCGCTGCC TCCTCCGGAA 
CATATGCCGG AAGCCTACTC AGCAAAAATT GCACTTTTTG GTGCAGGGCC TGCGAGTATA 
AGCTGTGCCT CCTTTTTGGC TCGGTTGGGC TATTCCAACA TTACTATATT CGAAAAACAA 
GAATATGTTG GTGGTTTAAG CACTTCTGAA ATCCCTCAGT TTCGACTCCC ATATGACGTC 
GTGAATTTTG AGATTGAGCT CATGAAGGAC CTTGGTGTAA AGATAATTTG TGGTAAAAGC 
CTTTCCACCG ATGAAATGAC TCTTAGCTCT TTGAAAGAAA ATGGCTACAG AGCTGCATTT 
ATTGGAATAG GCTTGCCAGA ACCCAAAAAG GACCATATTT TCCAAGGCTT GACACAAGTC 
CAGGGATTTT ACACATCTAA AGACTTTCTG CCACTTGTCG CCAAAAGCAG TAAAACAGGA 
ATGTGCGCCT GTCACTCTCC ATTGCCATCC ATAAGGGGAG CCGTGATTGT ACTCGGAGCT 
GGAGATACTG CGTTTGACTG TGCAACATCC GCTCTGCGCT GTGGAGCCCT TCGCGTGTTC 
ATCGTCTTCA GAAAGGGCTT TGTTAATATT CGAGCTGTTC CAGAGGAGAT GGAGCTCGCT 
AAGGAAGAGA AATGTGAATT TTTGCCTTTC CTCTCGCCAC GGAAGGTTAT AGTCAAAGAT 
GGAAAGATTG TGGCAATGCA ATTTGTTCGA ACTGAGCAAG ATGAAACCGG AAACTGGGTG 
GAAGATGAAG AGCAGACGGT GCGGCTAAAG GCTGATGTGG TTATTAGTGC CTTTGGATCT 
GTCCTGGAGG ATCCCAAAGT GAAAGAAGCA TTGAGTCCCA TCAAGTTTAA CAGATGGGGT 
CTCCCAGAAG TAAATCCAGA AACCATGCAA ACCAGTGAAC CATGGGTGTT TGCAGGTGGT 
GATGTTGTGG GTATGGCTAA CACCACAGTG GAATCTGTCA ACGATGGAAA GCAAGCTTCA 
TGGTACATTC ACAAGCACAT ACAGGCACAA TATGGAACCT CAGTGCCTTC TCAGCCTACA 
ATGCCCCTAT TTTACACTCC GGTTGACTTA GTGGACATCA GCGTGGAAAT GGCAGGGTTG 
AGGTTTCCCA ATCCCTTTGG CCTTGCCAGT GCGACACCAG CCACTAGCAC ACCAATGATT 
CGAAGGGCCT TTGAAGCTGG CTGGGGTTTT GCTCTGACCA AAACTTTCTC TCTTGATAAG 
GACATCGTGA CAAACGTCTC ACCAAGAATC ATCCGGGGGA CAACTTCTGG CCCCTTGTAT 
GGCCCTGGAC AAAGCTCCTT TCTTAACATT GAGCTCATCA GTGAGAAAAC AGCTGCATAT 
TGGTGTCACA GTGTCACCGA ACTAAAGGCT GACTTCCCGG ACAATATCCT GATCGCCAGC 
ATCATGTGCA GTTACAACAA GAGTGACTGG ATGGAACTCT CCAAAATGGC TGAGGCTTCT 
GGAGCAGATG CCCTGGAGTT AAATTTATCG TGTCCACATG GCATGGGGGA GAGAGGAATG 
GGCCTGGCTT GTGGGCAGGA TCCAGAGCTG GTGAGGAACA TCTGTCGCTG GGTTAGGCAA 
GCTGTTCGGG TTCCATTTTT TGCCAAGTTG ACCCCAAATG TCACTGATAT TGTAAGCATC 
GCAAGAGCAG CAAAGGAAGG TGGAGCAGAT GGTGTAACAG CCACCAACAC CGTCTCGGGT 
CTGATGGGAC TGAAAGCTGA TGGCACACCC TGGCCTGCTG TGGGCATTGG AAGGAGGACT 
ACATATGGAG GAGTGTCAGG AACTGCTATC AGGCCCATTG CTTTGAGAGC TGTGACCGCC 
ATTGCCCGTG CTTTGCCTGG GTTTCCTATT TTGGCCACAG GTGGAATTGA CTCAGCTGAA 
AGTGGACTTC AGTTTCTTCA TAGTGGTGCT TCAGTTCTTC AGGTATGCAG CGCTATTCAG 
AATCAGGACT TCACTGTGAT TGAAGATTAC TGCACTGGAC TCAAAGCCCT GCTTTATCTG 
AAGAGCATTG AAGAGTTAGC AGACTGGGAT GGTCAGAGTC CACCCATTAT AAGTCATCAG 
AAAGGGAAAC CAGTTCCACG CGTTGCTGAG CTCATGGGAC AGAAACTGCC CAGCTTTGGA 
CCTTACCTTG AACAGCGCAA GAAAATCATA GCAGCAAGCA AAATCAGACA GAAAGATCAA 
AACACAGCTT GCTCACCTCT CCAGAGAAAG CACTTTAACT CGCAAAAGCC TATTCCTGCC 
ATCAAGGATG TAATTGGAAA ATCACTGCAA TACCTGGGAA CATTTGGTGA GATGAGCATC 
ATGGAACAAG TTGTGGCCCT GATCGATGAG GAAATGTGCA TCAATTGCGG CAAATGTTAC 
ATGACCTGTA ATGACTCTGG CTACCAGGCT ATCCAGTTCG ATCCAGAAAC TCACCTGCCT 
ACCGTTAGCG ACACATGTAC AGGCTGTACT CTCTGCCTCA GCGTCTGCCC TATTATGGAC 
TGTATCAGGA TGGTTTCCAG GGCAACACCT TATCAACCAA AGAGAGGCCT ACCATTAGCC 
GTGAAGCCGG TGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bućan M,Murray SA

Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis.
Developmental biology406(1)74-91(2015 Oct)
Maddala R,Nagendran T,Lang RA,Morozov A,Rao PV

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Developmental biology360(2)415-25(2011 Dec)
Tamplin OJ,Cox BJ,Rossant J

Dihydropyrimidine dehydrogenase circadian rhythm in mouse liver: comparison between enzyme activity and gene expression.
European journal of cancer (Oxford, England : 1990)39(6)822-8(2003 Apr)
Porsin B,Formento JL,Filipski E,Etienne MC,Francoual M,Renée N,Magné N,Lévi F,Milano G

Regulation of dihydropyrimidine dehydrogenase gene expression in regenerating mouse liver.
International journal of oncology22(2)359-64(2003 Feb)
Yamada H,Fukushima M,Koizumi K,Katakura H,Nakanishi H,Teshima S,Kamiyama Y,Ikenaka K

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Dpyd cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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